Keiya Ozawa

Summary

Affiliation: Jichi Medical School
Country: Japan

Publications

  1. ncbi request reprint [Methods of gene transfer]
    Keiya Ozawa
    Division of Hematology, Department of Medicine, Jichi Medical School
    Nihon Rinsho 63:481-5. 2005
  2. doi request reprint Cell and gene therapy using mesenchymal stem cells (MSCs)
    Keiya Ozawa
    Division of Hematology, Department of Medicine, Jichi Medical University, 3311 1 Yakushiji, Shimotsuke shi, Tochigi 329 0498, Japan
    J Autoimmun 30:121-7. 2008
  3. pmc Japanese epidemiological survey with consensus statement on Japanese guidelines for treatment of iron overload in bone marrow failure syndromes
    Takahiro Suzuki
    Division of Hematology, Department of Medicine, Jichi Medical University, 3311 1 Yakushiji, Shimotsuke shi, Tochigi 329 0498, Japan
    Int J Hematol 88:30-5. 2008
  4. ncbi request reprint [Gene therapy using AAV]
    Keiya Ozawa
    Division of Hematology, Department of Medicine, Center for Molecular Medicine, Jichi Medical University, 3311 1 Yakushiji, Shimotsuke shi, Tochigi 329 0498, Japan
    Uirusu 57:47-55. 2007
  5. ncbi request reprint [Gene therapy using AAV (adeno-associated virus) vectors]
    Keiya Ozawa
    Division of Hematology, Department of Medicine, Center for Molecular Medicine, Jichi Medical School, 3311 1 Yakushiji, Minamikawachi Machi, Kawachi gun, Tochigi 329 0498, Japan
    Uirusu 53:163-70. 2003
  6. ncbi request reprint [Development of AAV vector and its application to gene therapy]
    Keiya Ozawa
    Tanpakushitsu Kakusan Koso 52:1288-93. 2007
  7. ncbi request reprint [Development and application of gene therapy technologies]
    Keiya Ozawa
    Division of Hematology, Department of Medicine, Jichi Medical School, 3311 1 Yakushiji, Minamikawachi machi Kawachi gun, Tochigi 329 0498, Japan
    Uirusu 54:49-57. 2004
  8. ncbi request reprint Efficient expression of a transgene in platelets using simian immunodeficiency virus-based vector harboring glycoprotein Ibalpha promoter: in vivo model for platelet-targeting gene therapy
    Tsukasa Ohmori
    Research Division of Cell and Molecular Medicine, Center for Molecular Medicine, Jichi Medical School, Minamikawachi, Tochigi 329 0498, Japan
    FASEB J 20:1522-4. 2006
  9. ncbi request reprint Utility of intraperitoneal administration as a route of AAV serotype 5 vector-mediated neonatal gene transfer
    Tsuyoshi Ogura
    Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical School, Tochigi, Japan
    J Gene Med 8:990-7. 2006
  10. ncbi request reprint Intramuscular injection of AAV-GDNF results in sustained expression of transgenic GDNF, and its delivery to spinal motoneurons by retrograde transport
    Yan Yan Lu
    Division of Neurology, Department of Medicine, Jichi Medical School, 3311 1 Yakushiji, Minamikawachi Machi, Tochigi 329 0498, Japan
    Neurosci Res 45:33-40. 2003

Detail Information

Publications109 found, 100 shown here

  1. ncbi request reprint [Methods of gene transfer]
    Keiya Ozawa
    Division of Hematology, Department of Medicine, Jichi Medical School
    Nihon Rinsho 63:481-5. 2005
  2. doi request reprint Cell and gene therapy using mesenchymal stem cells (MSCs)
    Keiya Ozawa
    Division of Hematology, Department of Medicine, Jichi Medical University, 3311 1 Yakushiji, Shimotsuke shi, Tochigi 329 0498, Japan
    J Autoimmun 30:121-7. 2008
    ..4) by using the integration machinery of adeno-associated virus (AAV) would be particularly valuable. There will be wide-ranging applications of MSCs to frontier medical treatments in the near future...
  3. pmc Japanese epidemiological survey with consensus statement on Japanese guidelines for treatment of iron overload in bone marrow failure syndromes
    Takahiro Suzuki
    Division of Hematology, Department of Medicine, Jichi Medical University, 3311 1 Yakushiji, Shimotsuke shi, Tochigi 329 0498, Japan
    Int J Hematol 88:30-5. 2008
    ....
  4. ncbi request reprint [Gene therapy using AAV]
    Keiya Ozawa
    Division of Hematology, Department of Medicine, Center for Molecular Medicine, Jichi Medical University, 3311 1 Yakushiji, Shimotsuke shi, Tochigi 329 0498, Japan
    Uirusu 57:47-55. 2007
    ..To this end, site-specific integration into the AAVS1 locus on the chromosome 19 (19q13.4) by using the integration machinery of AAV would be particularly valuable...
  5. ncbi request reprint [Gene therapy using AAV (adeno-associated virus) vectors]
    Keiya Ozawa
    Division of Hematology, Department of Medicine, Center for Molecular Medicine, Jichi Medical School, 3311 1 Yakushiji, Minamikawachi Machi, Kawachi gun, Tochigi 329 0498, Japan
    Uirusu 53:163-70. 2003
  6. ncbi request reprint [Development of AAV vector and its application to gene therapy]
    Keiya Ozawa
    Tanpakushitsu Kakusan Koso 52:1288-93. 2007
  7. ncbi request reprint [Development and application of gene therapy technologies]
    Keiya Ozawa
    Division of Hematology, Department of Medicine, Jichi Medical School, 3311 1 Yakushiji, Minamikawachi machi Kawachi gun, Tochigi 329 0498, Japan
    Uirusu 54:49-57. 2004
    ..Genetic manipulation is also one of the indispensable technologies in the field of regeneration medicine, and further promotion of basic research is important...
  8. ncbi request reprint Efficient expression of a transgene in platelets using simian immunodeficiency virus-based vector harboring glycoprotein Ibalpha promoter: in vivo model for platelet-targeting gene therapy
    Tsukasa Ohmori
    Research Division of Cell and Molecular Medicine, Center for Molecular Medicine, Jichi Medical School, Minamikawachi, Tochigi 329 0498, Japan
    FASEB J 20:1522-4. 2006
    ..Platelet-targeting gene therapy using SIV vectors appears to be promising for gene therapy approaches toward not only inherited platelet diseases but also other hemorrhagic disorders such as hemophilia A...
  9. ncbi request reprint Utility of intraperitoneal administration as a route of AAV serotype 5 vector-mediated neonatal gene transfer
    Tsuyoshi Ogura
    Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical School, Tochigi, Japan
    J Gene Med 8:990-7. 2006
    ..Here, we tested the usefulness of neonatal gene transfer using adeno-associated virus (AAV) vectors by comparing the administration routes and vector doses...
  10. ncbi request reprint Intramuscular injection of AAV-GDNF results in sustained expression of transgenic GDNF, and its delivery to spinal motoneurons by retrograde transport
    Yan Yan Lu
    Division of Neurology, Department of Medicine, Jichi Medical School, 3311 1 Yakushiji, Minamikawachi Machi, Tochigi 329 0498, Japan
    Neurosci Res 45:33-40. 2003
    ..This study provides a basis for a strategy of intramuscular AAV-GDNF delivery to protect motoneurons as a possible means of ALS treatment...
  11. ncbi request reprint Interleukin-10-mediated inhibition of angiogenesis and tumor growth in mice bearing VEGF-producing ovarian cancer
    Takahiro Kohno
    Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical School, Tochigi, 329 0498 Japan
    Cancer Res 63:5091-4. 2003
    ..Although the mechanisms of the antiangiogenic effect of IL-10 are still unclear, the potential usefulness of IL-10-mediated gene therapy of ovarian cancer was suggested...
  12. ncbi request reprint Suppression of ovarian cancer by muscle-mediated expression of soluble VEGFR-1/Flt-1 using adeno-associated virus serotype 1-derived vector
    Yuji Takei
    Division of Genetics Therapeutics, Center for Molecular Medicine, Jichi Medical School, Tochigi, Japan
    Int J Cancer 120:278-84. 2007
    ..These results indicate that sFlt-1 suppresses growth and peritoneal dissemination of ovarian cancer by the inhibition of angiogenesis, and thus suggest the usefulness of gene therapy for ovarian cancer...
  13. doi request reprint Suppression of lymph node and lung metastases of endometrial cancer by muscle-mediated expression of soluble vascular endothelial growth factor receptor-3
    Kayoko Takahashi
    Division of Genetics Therapeutics, Center for Molecular Medicine, Shimotsuke, Japan
    Cancer Sci 104:1107-11. 2013
    ..These results suggest the possibility of gene therapy against lymph node and lung metastases of endometrial cancer by using muscle-mediated expression of sVEGFR-3. ..
  14. ncbi request reprint Specific and efficient transduction of Cochlear inner hair cells with recombinant adeno-associated virus type 3 vector
    Yuhe Liu
    Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical School, Minami Kawachi, Kawachi, Tochigi, Japan
    Mol Ther 12:725-33. 2005
    ..These findings are of value for further molecular studies of cochlear inner hair cells and for gene replacement strategies to correct recessive genetic hearing loss due to monogenic mutations in these cells...
  15. ncbi request reprint Adenoassociated virus-mediated prostacyclin synthase expression prevents pulmonary arterial hypertension in rats
    Takayuki Ito
    Division of Genetic Therapeutics, Jichi Medical University, Tochigi, Japan
    Hypertension 50:531-6. 2007
    ..An intramuscular injection of AAV-PGIS prevents monocrotaline-pulmonary arterial hypertension in rats and provides a new therapeutic alternative for preventing pulmonary arterial hypertension in humans...
  16. doi request reprint Histone deacetylases are critical targets of bortezomib-induced cytotoxicity in multiple myeloma
    Jiro Kikuchi
    Division of Stem Cell Regulation, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Tochigi, Japan
    Blood 116:406-17. 2010
    ..These results suggest that bortezomib targets HDACs via distinct mechanisms from conventional HDAC inhibitors. Our findings provide a novel molecular basis and rationale for the use of bortezomib in MM treatment...
  17. ncbi request reprint Relative importance of apoptosis and cell cycle blockage in the synergistic effect of combined R115777 and imatinib treatment in BCR/ABL-positive cell lines
    Takuji Miyoshi
    Division of Hematology, Jichi Medical School, 3311 1 Yakushiji, Minamikawachi Machi, Kawachi gun, Tochigi 329 0498, Japan
    Biochem Pharmacol 69:1585-94. 2005
    ..Additional treatment for overriding the G1 checkpoint may be required to eradicate leukemia cells, in which the combination induces cell cycle arrest...
  18. ncbi request reprint Active FKHRL1 overcomes imatinib resistance in chronic myelogenous leukemia-derived cell lines via the production of tumor necrosis factor-related apoptosis-inducing ligand
    Satoru Kikuchi
    Division of Hematology, Department of Medicine, Jichi Medical University, Tochigi Ken 329 0498, Japan
    Cancer Sci 98:1949-58. 2007
    ..In addition, imatinib-resistant cells underwent apoptosis after transfection with full-length TRAIL cDNA. Collectively, our results suggest that active FKHRL1 can overcome imatinib resistance in CML cells, in part via TRAIL production...
  19. ncbi request reprint A histone deacetylase inhibitor enhances recombinant adeno-associated virus-mediated gene expression in tumor cells
    Takashi Okada
    Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical School, 3311 1 Yakushiji, Minami Kawachi, Tochigi 329 0498, Japan
    Mol Ther 13:738-46. 2006
    ..The use of this HDAC inhibitor may enhance the utility of rAAV-mediated transduction strategies for cancer gene therapy...
  20. ncbi request reprint Sustained transgene expression by human cord blood derived CD34+ cells transduced with simian immunodeficiency virus agmTYO1-based vectors carrying the human coagulation factor VIII gene in NOD/SCID mice
    Jiro Kikuchi
    Division of Cell and Molecular Medicine, The Center for Molecular Medicine, Jichi Medical School, Tochigi Ken 329 0498, Japan
    J Gene Med 6:1049-60. 2004
    ....
  21. ncbi request reprint Histone deacetylase inhibitor depsipeptide (FK228) induces apoptosis in leukemic cells by facilitating mitochondrial translocation of Bax, which is enhanced by the proteasome inhibitor bortezomib
    Krittaya Sutheesophon
    Division of Stem Cell Regulation, Center for Molecular Medicine, Jichi Medical School, Tochigi, Japan
    Acta Haematol 115:78-90. 2006
    ..The status of Bcl-2 and Bax may influence the sensitivity of tumors to this combination and thus can be a target of further therapeutic intervention...
  22. ncbi request reprint Interleukin-10 expression mediated by an adeno-associated virus vector prevents monocrotaline-induced pulmonary arterial hypertension in rats
    Takayuki Ito
    Division of Genetic Therapeutics, Jichi Medical University, 3311 1 Yakushiji, Shimotsuke, Tochigi 329 0498, Japan
    Circ Res 101:734-41. 2007
    ..Thus, IL-10 prevented the development of monocrotaline-induced PAH, and these results provide new insights into the molecular mechanisms of human PAH...
  23. ncbi request reprint Suppression of cell migration in ovarian cancer cells mediated by PTEN overexpression
    Yasushi Saga
    Department of Obstetrics and Gynecology, Jichi Medical School, Yakushiji, Minamikawachi Tochigi 329 0498, Japan
    Int J Oncol 23:1109-13. 2003
    ..The findings described above, show that enhanced expression of PTEN inhibits ovarian cancer cell migration, suggesting that gene therapy approaches using PTEN for control of peritoneal dissemination of ovarian cancer are possible...
  24. doi request reprint Retroviral vector-producing mesenchymal stem cells for targeted suicide cancer gene therapy
    Ryosuke Uchibori
    Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical University, Tochigi, Japan
    J Gene Med 11:373-81. 2009
    ..For efficient therapeutic application, we developed retroviral vector-producing MSCs that enhance tumor transduction via progeny vector production...
  25. ncbi request reprint Analysis of gene expression profiles in an imatinib-resistant cell line, KCL22/SR
    Ken Ohmine
    Divisions of Hematology, Cell Transplantation and Transfusion, and Functional Genomics, Jichi Medical School, Tochigi, Japan
    Stem Cells 21:315-21. 2003
    ..These results suggest that various mechanisms, including disturbance of Ras-mitogen-activated protein kinase signaling, are involved in imatinib resistance...
  26. ncbi request reprint Viral-mediated temporally controlled dopamine production in a rat model of Parkinson disease
    Xiao gang Li
    Division of Neurology, Department of Medicine, Center for Molecular Medicine, Jichi Medical School, Tochigi 329 0498, Japan
    Mol Ther 13:160-6. 2006
    ..Our data demonstrate that viral vector-mediated inducible Cre recombinase can serve as an in vivo molecular switch, allowing spatial and temporal control of transgene expression, thereby potentially increasing the safety of gene therapy...
  27. ncbi request reprint Ascorbic acid restores sensitivity to imatinib via suppression of Nrf2-dependent gene expression in the imatinib-resistant cell line
    Takahisa Tarumoto
    Division of Hematology, Jichi Medical School, Tochigi, Japan
    Exp Hematol 32:375-81. 2004
    ..Since oxidative stress promotes the translocation of Nrf2 from the cytoplasm to the nucleus, we also examined whether ascorbic acid, a reducing reagent, can overcome the resistance to imatinib by inhibiting Nrf2 activity...
  28. ncbi request reprint Repair of infarcted myocardium mediated by transplanted bone marrow-derived CD34+ stem cells in a nonhuman primate model
    Toru Yoshioka
    Center for Molecular Medicine, Department of Internal Medicine, Jichi Medical School, Minamikawachi, Tochigi 329 0498, Japan
    Stem Cells 23:355-64. 2005
    ....
  29. pmc Minimizing the inhibitory effect of neutralizing antibody for efficient gene expression in the liver with adeno-associated virus 8 vectors
    Jun Mimuro
    Division of Cell and Molecular Medicine, Center for Molecular Medicine, Jichi Medical University, Tochigi ken, Japan
    Mol Ther 21:318-23. 2013
    ..Our results suggest that portal vein-directed vector delivery strategies with flushing to remove blood are efficacious for minimizing the inhibitory effect of anti-AAV antibodies...
  30. ncbi request reprint In situ generation of pseudotyped retroviral progeny by adenovirus-mediated transduction of tumor cells enhances the killing effect of HSV-tk suicide gene therapy in vitro and in vivo
    Takashi Okada
    Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical School, Tochigi, Japan
    J Gene Med 6:288-99. 2004
    ....
  31. ncbi request reprint Tissue-targeted in vivo gene transfer coupled with histone deacetylase inhibitor depsipeptide (FK228) enhances adenoviral infection in rat renal cancer allograft model systems
    Minoru Kobayashi
    Department of Urology, Jichi Medical University, Tochigi, Japan
    Urology 70:1230-6. 2007
    ..To overcome this disadvantage, we performed in vivo local gene transfer, in combination with the histone deacetylase inhibitor, depsipeptide (FK228), in a rat renal cancer model...
  32. ncbi request reprint A soluble CAR-SCF fusion protein improves adenoviral vector-mediated gene transfer to c-Kit-positive hematopoietic cells
    Akira Itoh
    Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical School, Japan
    J Gene Med 5:929-40. 2003
    ....
  33. pmc Altered effector CD4+ T cell function in IL-21R-/- CD4+ T cell-mediated graft-versus-host disease
    Iekuni Oh
    Division of Hematology, Department of Medicine, Jichi Medical University, Tochigi, Japan
    J Immunol 185:1920-6. 2010
    ..Thus, the attenuated GVHD might be caused by an impairment of effector T cell differentiation itself, rather than by an increase in regulatory T cells and suppression of effector T cells...
  34. ncbi request reprint Topoisomerase inhibitors enhance the cytocidal effect of AAV-HSVtk/ganciclovir on head and neck cancer cells
    Takeharu Kanazawa
    Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical School, 3311 1 Yakushiji Minamikawachi, Tochigi 329 0498, Japan
    Int J Oncol 25:729-35. 2004
    ..These findings suggest that the combination of AAV-mediated suicide gene therapy and treatment with topoisomerase inhibitors may have synergistic therapeutic effects in the treatment of cancers...
  35. ncbi request reprint Gene transfer to the rat kidney in vivo and ex vivo using an adenovirus vector: factors influencing transgene expression
    Jun Fujishiro
    Division of Organ Replacement Research, Center for Molecular Medicine, Jichi Medical School, 3311 1 Yakushiji, Minamikawachi Machi, Tochigi 329 0498, Japan
    Nephrol Dial Transplant 20:1385-91. 2005
    ..We studied the effects of contact time and temperature on adenovirus-mediated transgene expression in rat kidneys, using catheter-based in vivo gene transfer and a rat renal transplant model ex vivo...
  36. doi request reprint Liver-restricted expression of the canine factor VIII gene facilitates prevention of inhibitor formation in factor VIII-deficient mice
    Akira Ishiwata
    Division of Cell and Molecular Medicine, Center for Molecular Medicine, Jichi Medical University, School of Medicine, Yakushiji, Shimotsuke, Japan
    J Gene Med 11:1020-9. 2009
    ..Gene therapy for hemophilia A with adeno-associated virus (AAV) vectors involves difficulties in the efficient expression of factor VIII (FVIII) and in antibody formation against transgene-derived FVIII...
  37. ncbi request reprint GATA suppresses erythropoietin gene expression through GATA site in mouse erythropoietin gene promoter
    Shigehiko Imagawa
    Division of Hematology, Institute of Clinical Medicine, University of Tsukuba, Ibaraki, Japan
    Int J Hematol 75:376-81. 2002
    ..These results indicate that mGATA binds to the TGATA site of the mEpo promoter and negatively regulates mEpo gene expression. Negative regulation of mEpo gene by GATA transcriptional factors is discussed...
  38. doi request reprint Preferential expression of phosphatidylglucoside along neutrophil differentiation pathway
    Satoko Oka
    Division of Cell Transplantation and Transfusion, Jichi Medical University, Tochigi, Shimotsuke, Japan
    Leuk Lymphoma 50:1190-7. 2009
    ..A positive correlation between PtdGlc expression and CD15 expression in leukemic cells from patients with acute myeloblastic leukemia was shown. These results indicate that increasing PtdGlc expression is seen with neutrophil maturation...
  39. doi request reprint Hemin reduces cellular sensitivity to imatinib and anthracyclins via Nrf2
    Tadashi Nagai
    Division of Hematology, Department of Medicine, Jichi Medical University, Tochigi, Japan
    J Cell Biochem 104:680-91. 2008
    ....
  40. ncbi request reprint Efficient gene transfer of a simian immuno-deficiency viral vector into cardiomyocytes derived from primate embryonic stem cells
    Mihoko Nagata
    Department of Medicine, Jichi Medical School, Tochigi 329 0498, Japan
    J Gene Med 5:921-8. 2003
    ..However, protocols for efficient gene transfer into primate ES-cell-derived cells have not yet been established...
  41. ncbi request reprint Hematopoietic microchimerism in sheep after in utero transplantation of cultured cynomolgus embryonic stem cells
    Kyoko Sasaki
    Division of Regenerative Medicine, Center for Molecular Medicine, Jichi Medical School, Tochigi, Japan
    Transplantation 79:32-7. 2005
    ..We first cultured cynomolgus monkey ES cells in vitro and transplanted the cells in vivo into fetal sheep liver, generating sheep with cynomolgus hematopoiesis...
  42. ncbi request reprint Transforming activity of purinergic receptor P2Y, G protein coupled, 8 revealed by retroviral expression screening
    Shin ichiro Fujiwara
    Divisions of Functional Genomics, Jichi Medical University, Tochigi, Japan
    Leuk Lymphoma 48:978-86. 2007
    ..Our data thus reveal an abundant expression of P2RY8 in leukemic cells and its unexpected role in the pathogenesis of acute leukemia...
  43. doi request reprint Cotransplantation with MSCs improves engraftment of HSCs after autologous intra-bone marrow transplantation in nonhuman primates
    Shigeo Masuda
    Division of Regenerative Medicine, Center for Molecular Medicine, Jichi Medical University, Tochigi 329 0498, Japan
    Exp Hematol 37:1250-1257.e1. 2009
    ..Here, using nonhuman primates, we investigated the effects of cotransplantation with MSCs on the engraftment of HSCs after autologous intra-bone marrow transplantation...
  44. doi request reprint Protection against aminoglycoside-induced ototoxicity by regulated AAV vector-mediated GDNF gene transfer into the cochlea
    Yuhe Liu
    Division of Genetic Therapeutics, Jichi Medical University, Tochigi, Japan
    Mol Ther 16:474-80. 2008
    ..Our results suggest that AAV1-mediated expression of GDNF using a regulated expression system in the cochlea is a promising strategy to protect the cochlea from aminoglycoside-induced damage...
  45. ncbi request reprint Adipose tissue as a novel target for in vivo gene transfer by adeno-associated viral vectors
    Hiroaki Mizukami
    Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Tochigi 329 0498, Japan
    Hum Gene Ther 17:921-8. 2006
    ..No damage was observed in the transduced tissue. Our results indicate that the proposed method is safe and efficient for gene transfer into adipose tissues, thus providing an alternative for supplemental gene therapy...
  46. ncbi request reprint Cloning and characterization of a human BCR/ABL-positive cell line, K562/RR, resistant to the farnesyltransferase inhibition by tipifarnib
    Takuji Miyoshi
    Division of Hematology, Department of Medicine, Jichi Medical University, Tochigi, Japan
    Exp Hematol 35:1358-65. 2007
    ..However, acquisition of resistance may be a problem for patients being treated with tipifarnib...
  47. ncbi request reprint Neuroprotective effects of glial cell line-derived neurotrophic factor mediated by an adeno-associated virus vector in a transgenic animal model of amyotrophic lateral sclerosis
    Li Jun Wang
    Division of Neurology, Department of Medicine, Center for Molecular Medicine, Jichi Medical School, Minamikawachi Machi, Tochigi 329 0498, Japan
    J Neurosci 22:6920-8. 2002
    ..Our finding thus indicates that AAV-mediated GDNF delivery to the muscle is a promising means of gene therapy for ALS...
  48. ncbi request reprint High-level in vivo gene marking after gene-modified autologous hematopoietic stem cell transplantation without marrow conditioning in nonhuman primates
    Kyoji Ueda
    Center for Molecular Medicine, Jichi Medical School, Tochigi 329 0498, Japan
    Mol Ther 10:469-77. 2004
    ..The increase was EPO-dependent, multilineage, polyclonal, and repeatable. Our results suggest that the combination of iBMT and SAG allows efficient in vivo gene transduction without marrow conditioning...
  49. ncbi request reprint Overexpression of a hybrid gene consisting of the amino-terminal fragment of urokinase and carboxyl-terminal domain of bikunin suppresses invasion and migration of human ovarian cancer cells in vitro
    Yuji Takei
    Department of Obstetrics and Gynecology, Jichi Medical School, Tochigi, Japan
    Int J Cancer 113:54-8. 2005
    ..The above findings suggest the applicability of therapeutic strategies targeting the inhibition of peritoneal invasion and dissemination of ovarian cancer by the use of the chimeric gene ATF-HI8...
  50. ncbi request reprint Erythropoietin overcomes imatinib-induced apoptosis and induces erythroid differentiation in TF-1/bcr-abl cells
    Mie Uchida
    Division of Hematology, Department of Medicine, Jichi Medical School, Minamikawachi Machi, Kawachi gun, Tochigi Ken 329 0498, Japan
    Stem Cells 22:609-16. 2004
    ..Taken together, imatinib-treated CML cells may differentiate into mature cells in the presence of differentiation-inducing cytokines such as EPO...
  51. doi request reprint Overexpression of factor VII ameliorates bleeding diathesis of factor VIII-deficient mice with inhibitors
    Atsushi Yasumoto
    Division of Cell and Molecular Medicine, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Tochigi Ken 329 0498, Japan
    Thromb Res 131:444-9. 2013
    ..Factor VIII (FVIII) treatment for hemophilia A has difficulties in correcting bleeding diathesis in the presence of inhibitors...
  52. pmc Novel anti-tumor mechanism of galanin receptor type 2 in head and neck squamous cell carcinoma cells
    Takayuki Uehara
    Department of Otorhinolaryngology, Head and Neck Surgery, Graduate School of Medicine, University of the Ryukyus, Nishihara, Japan Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Japan Department of Otolaryngology, Head and Neck Surgery, Jichi Medical University School of Medicine, Shimotsuke, Japan
    Cancer Sci 105:72-80. 2014
    ..These results illustrate that transient GALR2 expression in the presence of galanin induces apoptosis via diverse pathways and serves as a platform for suicide gene therapy against HNSCC...
  53. doi request reprint Clinical features of de novo CD25(+) diffuse large B-cell lymphoma
    Shin ichiro Fujiwara
    Division of Hematology, Department of Medicine, Jichi Medical University, Tochigi 329 0498, Japan
    Hematology 18:14-9. 2013
    ..CD25 may be a new prognostic marker and could be a therapeutic target in DLBCL...
  54. ncbi request reprint Hematopoietic recovery after administration of deferasirox for transfusional iron overload in a case of myelodysplastic syndrome
    Hiroshi Okabe
    Division of Hematology, Department of Medicine, Jichi Medical University
    Rinsho Ketsueki 50:1626-9. 2009
    ..This case suggests that excess iron hampers hematopoiesis and that adequate iron chelation may improve hematological data in some iron-overloaded patients...
  55. doi request reprint Cetuximab inhibits the growth of mucinous ovarian carcinoma tumor cells lacking KRAS gene mutations
    Naoto Sato
    Department of Obstetrics and Gynecology, Center for Molecular Medicine, School of Medicine, Jichi Medical University, Tochigi, Japan
    Oncol Rep 27:1336-40. 2012
    ..These results suggest the possibility of targeted molecular therapy with cetuximab for mucinous ovarian carcinoma cells lacking a KRAS gene mutation...
  56. doi request reprint Mesenchymal stromal cells inhibit Th17 but not regulatory T-cell differentiation
    Raine Tatara
    Division of Hematology, Department of Medicine, Jichi Medical University, Tochigi, Japan
    Cytotherapy 13:686-94. 2011
    ..We aimed to determine the effects of MSC on T helper 17 cells (Th17) and regulatory T-cell (T-reg) differentiation...
  57. ncbi request reprint Activation of FKHRL1 plays an important role in protecting erythroid cells from erythropoietin deprivation-induced apoptosis in a human erythropoietin-dependent leukemia cell line, UT-7/EPO
    Mie Uchida
    Division of Hematology, Department of Medicine, Jichi Medical University, Tochigi, Japan
    Int J Hematol 86:315-24. 2007
    ..This phenomenon appears to correspond to dauer formation in C elegans. Thus, the mechanism of lifespan extension may be broadly conserved from C elegans to humans...
  58. ncbi request reprint Adeno-associated viral vectors for Parkinson's disease
    Shin Ichi Muramatsu
    Division of Neurology, Jichi Medical School, Tochigi 329 0498, Japan
    Int Rev Neurobiol 55:205-22. 2003
  59. ncbi request reprint Distinct patterns of gene transfer to gerbil hippocampus with recombinant adeno-associated virus type 2 and 5
    Tatsuya Nomoto
    Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical School 3311 1 Yakushiji, Minami Kawachi, Kawachi, Tochigi 329 0498, Japan
    Neurosci Lett 340:153-7. 2003
    ..These findings would be valuable in optimizing rAAV-mediated gene transfer to the gerbil hippocampus...
  60. doi request reprint Combination of tipifarnib and rapamycin synergistically inhibits the growth of leukemia cells and overcomes resistance to tipifarnib via alteration of cellular signaling pathways
    Tadashi Nagai
    Division of Hematology, Department of Medicine, Jichi Medical University, 3311 1 Yakushiji, Shimotsuke, Tochigi, Japan
    Leuk Res 34:1057-63. 2010
    ..The results support the utility of this combination as a potential therapy for leukemia. The combination might also be effective in overcoming resistance to tipifarnib...
  61. doi request reprint Adeno-associated virus vector-mediated systemic interleukin-10 expression ameliorates hypertensive organ damage in Dahl salt-sensitive rats
    Mutsuko Nonaka-Sarukawa
    Division of Genetic Therapeutics, Jichi Medical University, Tochigi, Japan
    J Gene Med 10:368-74. 2008
    ..In the present study, we examined the preventive effects of adeno-associated virus (AAV) vector-mediated sustained IL-10 expression against hypertensive heart disease and renal dysfunction in Dahl salt-sensitive rats...
  62. ncbi request reprint Engraftment and tumor formation after allogeneic in utero transplantation of primate embryonic stem cells
    Takayuki Asano
    Division of Genetic Therapeutics, Jichi Medical School, Tochigi, Japan
    Transplantation 76:1061-7. 2003
    ....
  63. ncbi request reprint Autologous gamete cryopreservation before hemopoietic stem cell transplantation
    Takahiro Nagashima
    Jichi Medical School, Minamikawachi, Japan
    Med Sci Monit 11:CR91-4. 2005
    ..Infertility after hemopoietic stem cell transplantation (HST) is a serious problem for young patients. Autologous gamete collection before HST may be a promising strategy to overcome infertility...
  64. pmc Downregulation of indoleamine-2,3-dioxygenase in cervical cancer cells suppresses tumor growth by promoting natural killer cell accumulation
    Naoto Sato
    Department of Obstetrics and Gynecology, School of Medicine, Jichi Medical University, Tochigi, Japan
    Oncol Rep 28:1574-8. 2012
    ..These findings indicate that downregulation of IDO controls cervical cancer progression by activating NK cells, suggesting IDO as a potential therapy for cervical cancer...
  65. ncbi request reprint Transforming activity of the lymphotoxin-beta receptor revealed by expression screening
    Shin ichiro Fujiwara
    Division of Functional Genomics, Jichi Medical School, Tochigi 329 0498, Japan
    Biochem Biophys Res Commun 338:1256-62. 2005
    ..These observations suggest that LTBR, which belongs to the tumor necrosis factor receptor superfamily of proteins, may contribute to human carcinogenesis...
  66. ncbi request reprint Overexpression of thymidylate synthase mediates desensitization for 5-fluorouracil of tumor cells
    Yasushi Saga
    Department of Obstetrics and Gynecology, Jichi Medical School, Yakushiji, Minamikawachi, Tochigi 329 0498, Japan
    Int J Cancer 106:324-6. 2003
    ..6 microM), showing significantly decreased sensitivity to 5-FU (p < 0.05). Thus, TS-overexpressing tumors have decreased sensitivity to 5-FU, which may be one of the factors that determine the prognosis of these tumors...
  67. ncbi request reprint Nitric oxide plays a critical role in suppression of T-cell proliferation by mesenchymal stem cells
    Kazuya Sato
    Division of Hematology, Jichi Medical University, 3311 1 Yakushiji, Tochigi 329 0498, Japan
    Blood 109:228-34. 2007
    ..Finally, MSCs from inducible NOS-/- mice had a reduced ability to suppress T-cell proliferation. Taken together, these results suggest that NO produced by MSCs is one of the major mediators of T-cell suppression by MSCs...
  68. ncbi request reprint Removal of empty capsids from type 1 adeno-associated virus vector stocks by anion-exchange chromatography potentiates transgene expression
    Masashi Urabe
    Division of Genetic Therapeutics, Jichi Medical School, 3311 1 Yakushiji, Tochigi 329 0498, Japan
    Mol Ther 13:823-8. 2006
    ..These results suggest that chromatographic separation of rAAV1 from empty capsids increased the efficacy of rAAV1...
  69. ncbi request reprint Development of new inbred transgenic strains of rats with LacZ or GFP
    Hirokazu Inoue
    Division of Organ Replacement Research, Center for Molecular Medicine, Jichi Medical School, Tochigi 329 0498, Japan
    Biochem Biophys Res Commun 329:288-95. 2005
    ..Our Tg-rat system may provide great benefits for the elucidation of the cellular process of regenerative medicine, including cell and tissue transplantation...
  70. ncbi request reprint Phenotype correction of hemophilia A mice with adeno-associated virus vectors carrying the B domain-deleted canine factor VIII gene
    Akira Ishiwata
    Division of Cell and Molecular Medicine, The Center for Molecular Medicine, Jichi Medical School, Tochigi ken, Japan
    Thromb Res 118:627-35. 2006
    ..These data suggested that both AAV1 and AAV8 vectors carrying the FVIII gene utilizing a minimum promoter have a potential for hemophilia A gene therapy...
  71. ncbi request reprint Pleocytosis after hemopoietic stem cell transplantation
    Takahiro Nagashima
    Division of Cell Therapy, Jichi Medical School Hospital, Minamikawachi Machi, Kawachi gun, Tochigi ken, Japan
    Leuk Lymphoma 47:1613-7. 2006
    ..In patients receiving allogeneic HSC transplants, CSF pleocytosis may be relatively common and may reflect neurologic damage associated with calcineurin inhibitors...
  72. doi request reprint Rituximab plus 70% cyclophosphamide, doxorubicin, vincristine and prednisone for Japanese patients with diffuse large B-cell lymphoma aged 70 years and older
    Akiko Meguro
    Division of Hematology, Department of Medicine, Jichi Medical University, Tochigi, Japan
    Leuk Lymphoma 53:43-9. 2012
    ....
  73. doi request reprint Vincristine potentiates the anti-proliferative effect of an aurora kinase inhibitor, VE-465, in myeloid leukemia cells
    Kozue Yoshida
    Jichi Medical University, 3311 1 Yakushiji, Shimotsuke, Tochigi 329 0498, Japan
    Biochem Pharmacol 82:1884-90. 2011
    ..In conclusion, our results indicate the utility of the combination of VE-465 and vincristine as a potential therapy for myeloid leukemia...
  74. ncbi request reprint Overexpression of PTEN increases sensitivity to SN-38, an active metabolite of the topoisomerase I inhibitor irinotecan, in ovarian cancer cells
    Yasushi Saga
    Department of Obstetrics and Gynecology, Jichi Medical School, Yakushiji, Minamikawachi, Tochigi 329 0498, Japan
    Clin Cancer Res 8:1248-52. 2002
    ..It is for the purpose of this study to investigate its function and the mechanisms by which PTEN enhances the sensitivity of ovarian cancer to antitumor agents...
  75. doi request reprint Correlation between flow cytometric identification of CD33-positive cells and morphological evaluation of myeloblasts in bone marrow of patients with acute myeloblastic leukemia
    Satoko Oka
    Division of Hematology, Department of Medicine, Jichi Medical University, Shimotsuke, Tochigi, Japan
    Hematology 14:133-8. 2009
    ..672, p<0.001). The identification of CD33+ cells by FCM/CD45 is useful for the evaluation of bone marrow myeloblasts in AML...
  76. pmc Scalable generation of high-titer recombinant adeno-associated virus type 5 in insect cells
    Masashi Urabe
    Division of Genetic Therapeutics, Jichi Medical School, 3311 1 Yakushiji, Minami Kawachi, Tochigi 329 0498, Japan
    J Virol 80:1874-85. 2006
    ..These results indicated that rAAV5 can be generated in insect cells, although the difference in incorporated virion DNA may induce different expression patterns of the transgene...
  77. ncbi request reprint Establishment and characterization of a new erythroblastic leukemia cell line, EEB: phosphatidylglucoside-mediated erythroid differentiation and apoptosis
    Chizuru Kawano-Yamamoto
    Division of Hematology, Department of Medicine, Jichi Medical School, Minamikawachi, Tochigi 329 0498, Japan
    Leuk Res 30:829-39. 2006
    ..The GL-7-mediated erythroid differentiation was associated with apoptosis. These results suggest that direct signaling to PhGlc mediates erythroid differentiation and apoptosis in EEB cells...
  78. ncbi request reprint [Hematopoietic malignancies and gene therapy]
    Keiya Ozawa
    Division of Hematology, Dept of Medicine, Jichi Medical School
    Gan To Kagaku Ryoho 30:478-82. 2003
    ..This genetic event is considered to be a trigger of T-lymphocytic leukemia development. Further basic studies are needed in terms of safety for stem cell gene therapy...
  79. ncbi request reprint Identification of the human erythropoietin receptor region required for Stat1 and Stat3 activation
    Keita Kirito
    Division of Hematology, Department of Medicine, Jichi Medical School, Tochigi, Japan
    Blood 99:102-10. 2002
    ..In addition, Jak2 and Fes tyrosine kinases were involved in EPO-induced activation of Stat1 and Stat3. These results indicate that Stat1 and Stat3 are activated by EPO via distinct mechanisms from Stat5...
  80. ncbi request reprint Highly efficient gene transfer into primate embryonic stem cells with a simian lentivirus vector
    Takayuki Asano
    Division of Genetic Therapeutics, Jichi Medical School, Tochigi, 329 0498, Japan
    Mol Ther 6:162-8. 2002
    ..Furthermore, this highly efficient gene transfer method allows faithful gene delivery to primate ES cells with potential for both research and therapeutic application...
  81. ncbi request reprint Separate control of Rep and Cap expression using mutant and wild-type loxP sequences and improved packaging system for adeno-associated virus vector production
    Hiroaki Mizukami
    Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical School, 3311 1, Yakushiji, Minamikawachi Machi, Kawachi gun, Tochigi, 329 0498, Japan
    Mol Biotechnol 27:7-14. 2004
    ..1 x 1010 genome copies per 10-cm dish was obtained. These results indicate the importance of controlling Cap expression, in addition to Rep, to achieve an optimum production rate for AAV vectors...
  82. ncbi request reprint [AAV vector-mediated gene transfer and its application to the nervous system]
    Keiya Ozawa
    Division of Hematology, Department of Medicine, Center for Molecular Medicine, Jichi Medical School
    Rinsho Shinkeigaku 43:835-8. 2003
    ..Preclinical studies are being conducted in MPTP-parkinsonian monkeys. AAV vector-mediated gene therapy would be feasible as a novel treatment of PD in the near future...
  83. pmc Roles of Bim in apoptosis of normal and Bcr-Abl-expressing hematopoietic progenitors
    Ryoko Kuribara
    Department of Hematology, Jichi Medical School, Tochigi, Japan
    Mol Cell Biol 24:6172-83. 2004
    ..These findings suggest that Bim plays an important role in the apoptosis of early hematopoietic progenitors and that Bcr-Abl supports cell survival in part through downregulation of this cell death activator...
  84. ncbi request reprint Thrombopoietin regulates Bcl-xL gene expression through Stat5 and phosphatidylinositol 3-kinase activation pathways
    Keita Kirito
    Division of Hematology, Department of Medicine, Jichi Medical School, Tochigi 329 04, Japan
    J Biol Chem 277:8329-37. 2002
    ..In addition, our data suggest possible cross-talk between these two signaling pathways...
  85. ncbi request reprint Soluble FLT-1 expression suppresses carcinomatous ascites in nude mice bearing ovarian cancer
    Yoko Hasumi
    Division of Genetic Therapeutics, Jichi Medical School, Tochigi 329 0498, Japan
    Cancer Res 62:2019-23. 2002
    ..These results suggest that inhibition of VEGF activity by sFlt-1 expression may provide a means to control carcinomatous ascites and angiogenesis of malignant ascites tumors...
  86. ncbi request reprint AAV-mediated VEGF gene transfer into skeletal muscle stimulates angiogenesis and improves blood flow in a rat hindlimb ischemia model
    Masahisa Shimpo
    Division of Cardiovascular Medicine, Jichi Medical School, Minamikawachi Machi, Tochigi 329 0498, Japan
    Cardiovasc Res 53:993-1001. 2002
    ..To establish a more efficient gene transfer method for ischemic diseases, we investigated the therapeutic potential of adeno-associated virus (AAV)-mediated VEGF gene transfer...
  87. ncbi request reprint In vivo expansion of transduced murine hematopoietic cells with a selective amplifier gene
    Akihiro Kume
    Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical School, Tochigi 329 0498, Japan
    J Gene Med 5:175-81. 2003
    ..Prototype SAGs conferred estrogen-responsive growth on murine hematopoietic progenitors...
  88. ncbi request reprint E2F-6 suppresses growth-associated apoptosis of human hematopoietic progenitor cells by counteracting proapoptotic activity of E2F-1
    Jiro Kikuchi
    Division of Stem Cell Regulation, Center for Molecular Medicine, Jichi Medical School, 3311 1 Yakushiji, Shimotsuke City, Tochigi 329 0498, Japan
    Stem Cells 25:2439-47. 2007
    ..These results suggest that E2F-6 provides a failsafe mechanism against loss of hematopoietic progenitor cells during proliferation. Disclosure of potential conflicts of interest is found at the end of this article...
  89. ncbi request reprint DNA microarray analysis of stage progression mechanism in myelodysplastic syndrome
    Masuzu Ueda
    Division of Hematology, Jichi Medical School, Kawachigun, Tochigi, Japan
    Br J Haematol 123:288-96. 2003
    ..Induction of PIASy expression in a mouse myeloid cell line induced apoptosis. A loss of PIASy expression may therefore contribute directly to the growth of MDS blasts and stage progression...
  90. ncbi request reprint Cetuximab inhibits growth, peritoneal dissemination, and lymph node and lung metastasis of endometrial cancer, and prolongs host survival
    Kayoko Takahashi
    Department of Obstetrics and Gynecology, School of Medicine, Jichi Medical University, Tochigi, Japan
    Int J Oncol 35:725-9. 2009
    ..In addition, the antibody prolonged the survival of a mouse model of systemic metastasis. These results suggest the possibility of molecular-targeted therapy using cetuximab for endometrial cancer...
  91. doi request reprint Establishment and characterization of transplantable, luminescence labeled rat renal cell carcinoma cell lines
    Minoru Kobayashi
    Department of Urology, Center for Molecular Medicine, Jichi Medical University, Tochigi, Japan
    J Urol 183:2029-35. 2010
    ..Recent advances in bioluminescence imaging permit sensitive in vivo detection and quantification of cells emitting light. Thus, we established bioluminescent rat renal cell carcinoma cell lines for immunocompetent rats...
  92. doi request reprint Incidental carcinomas detected by PET/CT scans in patients with malignant lymphoma
    Kazuya Sato
    Division of Hematology, Jichi Medical University, 3311 1 Yakushiji, Shimotsuke, Tochigi 329 0498, Japan
    Int J Hematol 92:647-50. 2010
    ..All of the 4 patients with colon carcinoma underwent curative surgery. The present study suggests that incidental findings by PET in malignant lymphoma can lead to early detection and successful treatment of second malignancies...
  93. doi request reprint Cut-off value of red-blood-cell-bound IgG for the diagnosis of Coombs-negative autoimmune hemolytic anemia
    Toyomi Kamesaki
    Center for Community Medicine, Jichi Medical University, Tochigi, Japan
    Am J Hematol 84:98-101. 2009
    ..5 and showed 100% sensitivity and 94% specificity, independent of gender and age. Results indicate that RBC-IgG could become a standard approach for the diagnosis of Coombs-negative AIHA, when measured before treatment...
  94. ncbi request reprint Behavioral recovery in a primate model of Parkinson's disease by triple transduction of striatal cells with adeno-associated viral vectors expressing dopamine-synthesizing enzymes
    Shin Ichi Muramatsu
    Department of Neurology, Jichi Medical School, Tochigi, 329 0498 Japan
    Hum Gene Ther 13:345-54. 2002
    ..Our results show that AAV vectors efficiently introduce DA-synthesizing enzyme genes into the striatum of primates with restoration of motor functions. This triple transduction method may offer a potential therapeutic strategy for PD...
  95. ncbi request reprint Selective expansion of transduced cells for hematopoietic stem cell gene therapy
    Akihiro Kume
    Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical School, Minamikawachi, Tochigi, Japan
    Int J Hematol 76:299-304. 2002
    ..This review aims at an overview of recent advances involving these strategies, with some details of "selective amplifier genes," a novel system that we have developed for specific expansion of genetically modified hematopoietic cells...
  96. ncbi request reprint Two-color flow cytometry with a CD19 gate for the evaluation of bone marrow involvement of B-cell lymphoma
    Chizuru Kawano-Yamamoto
    Division of Cell Transplantation and Transfusion, Jichi Medical School, Minamikawachi, Tochigi 329 0498, Japan
    Leuk Lymphoma 43:2133-7. 2002
    ..These results suggest that CD19-FCM is useful for the evaluation of bone marrow involvement of B-cell lymphoma...
  97. ncbi request reprint Successful gene transfer using adeno-associated virus vectors into the kidney: comparison among adeno-associated virus serotype 1-5 vectors in vitro and in vivo
    Shin Ichi Takeda
    Division of Organ Replacement Research, Center for Molecular Medicine, Jichi Medical School, Tochigi, Japan
    Nephron Exp Nephrol 96:e119-26. 2004
    ..We explored the transduction efficiency of renal cells in vitro and in vivo using adeno-associated virus (AAV) serotype 1-5 vectors encoding the beta-galactosidase gene...
  98. pmc Frag1, a homolog of alternative replication factor C subunits, links replication stress surveillance with apoptosis
    Hideshi Ishii
    Center for Molecular Medicine, Jichi Medical School, Tochigi 329 0498, Japan
    Proc Natl Acad Sci U S A 102:9655-60. 2005
    ....
  99. ncbi request reprint Overexpression of interleukin 21 induces expansion of hematopoietic progenitor cells
    Katsutoshi Ozaki
    Division of Hematopoietic Factors, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan
    Int J Hematol 84:224-30. 2006
    ..These results demonstrate that IL-21 can induce the expansion of hematopoietic progenitor cells in vivo, even in the absence of mature T-cells and B-cells...
  100. ncbi request reprint Retrospective nationwide survey of Japanese patients with transfusion-dependent MDS and aplastic anemia highlights the negative impact of iron overload on morbidity/mortality
    Masaaki Takatoku
    Division of Hematology, Department of Medicine, Jichi Medical University, Tochigi, Japan
    Eur J Haematol 78:487-94. 2007
    ..This retrospective survey investigated relationships between iron overload, chelation practices, and morbidity/mortality in patients with these diseases...
  101. ncbi request reprint Current drug targets and future therapy of pulmonary arterial hypertension
    Takayuki Ito
    Division of Genetic Therapeutics, Center for Molecular Medicine, Department of Medicine, Jichi Medical University, 3311 1 Yakushiji, Shimotsuke, Tochigi 329 0498, Japan
    Curr Med Chem 14:719-33. 2007
    ..Collaboration for integrated research will address these issues and generate greater clinical benefits for PAH patients...