Youji Shimazaki

Summary

Affiliation: Ehime University
Country: Japan

Publications

  1. ncbi Protein spot recognition on the non-denaturing and denaturing two-dimensional electrophoresis patterns using in situ immunosubtraction via Protein A agarose and antibodies
    Y Shimazaki
    Department of Material Science, Faculty of Science, Ehime University, Matsuyama, Japan
    J Biochem Biophys Methods 39:179-84. 1999
  2. doi Simultaneous production of immunoaffinity membranes
    Youji Shimazaki
    Graduate School of Science and Engineering Science Section and Venture Business Laboratory, Ehime University, Matsuyama, Japan
    J Chromatogr B Analyt Technol Biomed Life Sci 878:2852-6. 2010
  3. doi Enzyme separation and isozyme heterogeneity analysis using non-denaturing two-dimensional electrophoresis
    Youji Shimazaki
    Graduate School of Science and Engineering Science Section and Venture Business Laboratory, Ehime University, Matsuyama, Japan
    Talanta 82:1063-7. 2010
  4. ncbi Determination of malic Acid using a malate dehydrogenase reactor after purification and immobilization in non-denaturing conditions and staining with ponceau S
    Youji Shimazaki
    Graduate School of Science and Engineering Science Section and Venture Business Laboratory, Ehime University, Matsuyama, Japan
    Protein Pept Lett 17:1048-52. 2010
  5. doi Production of enzyme reactors after separation by non-denaturing two-dimensional electrophoresis and immobilization on membrane
    Youji Shimazaki
    Graduate School of Science and Engineering Science Section and Venture Business Laboratory, Ehime University, Matsuyama City, 790 8577, Japan
    Biotechnol Lett 31:1545-9. 2009
  6. doi Production of immunoaffinity membranes for direct analysis of antigen after antibody separation and blotting under non-denaturing conditions
    Youji Shimazaki
    Graduate School of Science and Engineering Science Section and Venture Business Laboratory, Ehime University, Bunkyo cho 2 5, Matsuyama City 790 8577, Japan
    Anal Chim Acta 643:61-6. 2009
  7. doi Simultaneous immobilization of dehydrogenases on polyvinylidene difluoride resin after separation by non-denaturing two-dimensional electrophoresis
    Youji Shimazaki
    Graduate School of Science and Engineering Science Section and Venture Business Laboratory, Ehime University, Bunkyo cho 2 5, Matsuyama City 790 8577, Japan
    Anal Chim Acta 618:116-9. 2008
  8. doi Silver staining of an esterase compatible with activity and mass spectrometry analysis after separation using non-denaturing two-dimensional electrophoresis
    Youji Shimazaki
    Graduate School of Science and Engineering Science Section and Venture Business Laboratory, Ehime University, Matsuyama City 790 8577, Japan
    Clin Chim Acta 390:145-7. 2008
  9. ncbi Direct analysis of retinal dehydrogenase activity on an electroblotting membrane following separation by non-denaturing two-dimensional electrophoresis
    Youji Shimazaki
    Graduate School of Science and Engineering Science Section and Venture Business Laboratory, Ehime University, Matsuyama, Japan
    J Chromatogr B Analyt Technol Biomed Life Sci 860:180-4. 2007
  10. ncbi Analysis of lipid hydrolytic activity by esterase on blotting membrane followed by separation using non-denaturing two-dimensional gel electrophoresis
    Youji Shimazaki
    Graduate School of Science and Engineering Science Section, Venture Business Laboratory, Ehime University, Matsuyama City 790 8577, Japan
    Biotechnol Bioeng 98:732-6. 2007

Collaborators

Detail Information

Publications31

  1. ncbi Protein spot recognition on the non-denaturing and denaturing two-dimensional electrophoresis patterns using in situ immunosubtraction via Protein A agarose and antibodies
    Y Shimazaki
    Department of Material Science, Faculty of Science, Ehime University, Matsuyama, Japan
    J Biochem Biophys Methods 39:179-84. 1999
    ..The technique can be applied to the extraction and analysis of proteins in small amounts of samples...
  2. doi Simultaneous production of immunoaffinity membranes
    Youji Shimazaki
    Graduate School of Science and Engineering Science Section and Venture Business Laboratory, Ehime University, Matsuyama, Japan
    J Chromatogr B Analyt Technol Biomed Life Sci 878:2852-6. 2010
    ..The results indicated that membrane-immobilized antibodies are simultaneously produced, and that the immunoaffinity membranes can capture specific substances in flowing fluids...
  3. doi Enzyme separation and isozyme heterogeneity analysis using non-denaturing two-dimensional electrophoresis
    Youji Shimazaki
    Graduate School of Science and Engineering Science Section and Venture Business Laboratory, Ehime University, Matsuyama, Japan
    Talanta 82:1063-7. 2010
    ..The results of this study indicate that non-denaturing 2-DE can be applied to both enzyme separation and isozyme heterogeneity analysis...
  4. ncbi Determination of malic Acid using a malate dehydrogenase reactor after purification and immobilization in non-denaturing conditions and staining with ponceau S
    Youji Shimazaki
    Graduate School of Science and Engineering Science Section and Venture Business Laboratory, Ehime University, Matsuyama, Japan
    Protein Pept Lett 17:1048-52. 2010
    ..These results indicate that enzyme reactors can be systematically produced after purification, immobilization and staining with Ponceau S...
  5. doi Production of enzyme reactors after separation by non-denaturing two-dimensional electrophoresis and immobilization on membrane
    Youji Shimazaki
    Graduate School of Science and Engineering Science Section and Venture Business Laboratory, Ehime University, Matsuyama City, 790 8577, Japan
    Biotechnol Lett 31:1545-9. 2009
    ..Hydrolytic activity of the esterase on the membranes was 20% of the original activity of the tissue homogenate. The present method can be applied to the production of several types of enzyme reactors on membranes...
  6. doi Production of immunoaffinity membranes for direct analysis of antigen after antibody separation and blotting under non-denaturing conditions
    Youji Shimazaki
    Graduate School of Science and Engineering Science Section and Venture Business Laboratory, Ehime University, Bunkyo cho 2 5, Matsuyama City 790 8577, Japan
    Anal Chim Acta 643:61-6. 2009
    ..We found that immunoaffinity membranes can be useful for simple and rapid removal and extraction of intact proteins after antibody separation and blotting under non-denaturing conditions...
  7. doi Simultaneous immobilization of dehydrogenases on polyvinylidene difluoride resin after separation by non-denaturing two-dimensional electrophoresis
    Youji Shimazaki
    Graduate School of Science and Engineering Science Section and Venture Business Laboratory, Ehime University, Bunkyo cho 2 5, Matsuyama City 790 8577, Japan
    Anal Chim Acta 618:116-9. 2008
    ..In conclusion, resin-immobilized dehydrogenases can be simultaneously obtained after separation by non-denaturing 2-DE, detection by negative staining and transferring to resins...
  8. doi Silver staining of an esterase compatible with activity and mass spectrometry analysis after separation using non-denaturing two-dimensional electrophoresis
    Youji Shimazaki
    Graduate School of Science and Engineering Science Section and Venture Business Laboratory, Ehime University, Matsuyama City 790 8577, Japan
    Clin Chim Acta 390:145-7. 2008
    ....
  9. ncbi Direct analysis of retinal dehydrogenase activity on an electroblotting membrane following separation by non-denaturing two-dimensional electrophoresis
    Youji Shimazaki
    Graduate School of Science and Engineering Science Section and Venture Business Laboratory, Ehime University, Matsuyama, Japan
    J Chromatogr B Analyt Technol Biomed Life Sci 860:180-4. 2007
    ..The results indicated that the reported methods could be applied for the direct examination of changes in retinoid catalyzed by enzymes on such membranes...
  10. ncbi Analysis of lipid hydrolytic activity by esterase on blotting membrane followed by separation using non-denaturing two-dimensional gel electrophoresis
    Youji Shimazaki
    Graduate School of Science and Engineering Science Section, Venture Business Laboratory, Ehime University, Matsuyama City 790 8577, Japan
    Biotechnol Bioeng 98:732-6. 2007
    ..These results also indicate that a micro-immobilized enzyme reactor on the membrane can be produced when biological enzymes are separated by microscale 2DE, transferred to the membrane and stained without impairing their activities...
  11. ncbi Analysis of hydrolytic activity of phospholipase Calpha from porcine retina on retinyl ester and phosphatidylcholine using non-denaturing two-dimensional electrophoresis and mass spectrometry
    Youji Shimazaki
    Faculty of Science and Venture Business Laboratory, Ehime University, Matsuyama, Japan
    J Chromatogr B Analyt Technol Biomed Life Sci 843:42-6. 2006
    ..Results indicated that hydrolytic activity of the enzyme with non-native and native substrates could be examined using a combination of non-denaturing 2DE and MALDI-TOF-MS...
  12. ncbi Hydrolytic activity of lipase on anion-exchange solid phase column after separation and electrotransfer by non-denaturing electrophoresis
    Youji Shimazaki
    Graduate School of Science and Engineering Science Section and Venture Business Laboratory, Ehime University, Matsuyama City, 790 8577, Japan
    J Biochem Biophys Methods 70:487-91. 2007
    ..The results indicated that a column with hydrolytic activity could be produced once lipases separated by non-denaturing 2-DE were transferred to the solid phase column...
  13. ncbi Removal of specific protein spots on the patterns of non-denaturing two-dimensional electrophoresis using protein A agarose and antibodies
    Y Shimazaki
    Department of Material Science, Faculty of Science, Ehime University, Mastuyama city, Japan
    J Biochem Biophys Methods 37:1-4. 1998
    ..A protein residing at pI 5.8 and with a molecular mass of about 200 kDa was clearly detected when the IgG and IgA spots were removed from the 2-D PAGE pattern...
  14. ncbi Isolation of the third component of complement and its derivative with anaphylatoxin-like activity from the plasma of the newt Cynops pyrrhogaster
    Y Shimazaki
    Department of Material Science, Faculty of Science, Ehime University, Matsuyama, Japan
    Dev Comp Immunol 25:467-74. 2001
    ..Digestion of newt C3 with trypsin yielded fragments that induced significant histamine release from newt peritoneal cells. These results indicate that newt C3 retains structural and functional properties shared with mammalian C3...
  15. ncbi Simple quantification of Cu,Zn-superoxide dismutase activity after separation by non-denaturing isoelectric focusing
    Youji Shimazaki
    Department of Material Science, Faculty of Science and Venture Business Laboratory, Ehime University, Matsuyama City, 790 8577 Japan
    Biochim Biophys Acta 1571:245-8. 2002
    ..After the SOD and pigment in the retina cytosol are separated, SOD activity can be quantified by water-soluble tetrazolium salt. We also found that SOD separated by this IEF retained its native function...
  16. ncbi Analysis of the activity and identification of enzymes after separation of cytosol proteins in mouse liver by microscale nondenaturing two-dimensional electrophoresis
    Youji Shimazaki
    Department of Physics and Chemistry, Faculty of Science and Venture Business Laboratory, Ehime University, Matsuyama, Japan
    Proteomics 3:2002-7. 2003
    ..Present results also indicate analysis of enzyme activity using nondenaturing 2-DE can be applied to screen substances which affect enzyme activity...
  17. ncbi Simultaneous analysis of esterase and transferase activities in cytosol proteins from the bovine retina by using microscale non-denaturing two-dimensional electrophoresis
    Youji Shimazaki
    Department of Chemistry, Faculty of Science and Venture Business Laboratory, Ehime University, Matsuyama 790 8577, Japan
    Biochim Biophys Acta 1696:51-7. 2004
    ..This simultaneous analysis of enzyme activities can be applied to screen-specific or non-specific medicines which affect enzyme activities...
  18. ncbi Activity and sequence structure analysis of cytosolic dehydrogenase by mass spectrometry after separation by nondenaturing two-dimensional electrophoresis
    Youji Shimazaki
    Department of Physics and Chemistry, Faculty of Science and Venture Business Laboratory, Ehime University, Matsuyama City 790 8577, Japan
    Anal Biochem 328:87-9. 2004
  19. ncbi Nondenaturing two-dimensional electrophoresis enzyme profile involving activity and sequence structure of cytosol proteins from mouse liver
    Youji Shimazaki
    Department of Physics and Chemistry, Faculti of Science and Venture Business Laboratory, Ehime University, Matsuyama, Japan
    Proteomics 4:1406-11. 2004
    ..This profile can be used for checking whether activities of enzymes were specifically or nonspecifically inhibited by inhibitors...
  20. ncbi Detection of activity and mass spectrometric identification of mouse liver carboxylesterase and aldehyde dehydrogenase separated by non-denaturing two-dimensional electrophoresis after extraction with detergents
    Youji Shimazaki
    Department of Chemistry, Faculty of Science and Venture Business Laboratory, Ehime University, Matsuyama, Japan
    Biochim Biophys Acta 1749:95-101. 2005
    ..These results indicate that the activities and identity of detergent-soluble enzymes can be examined by this non-denaturing 2-DE and mass spectrometry...
  21. doi Epitope analysis using membrane-immobilized avidin and protein A
    Youji Shimazaki
    Graduate School of Science and Engineering, Science Section, and Venture Business Laboratory, Ehime University, Matsuyama, Japan
    Protein Expr Purif 83:177-81. 2012
    ..These results thus indicate that the combined methods of membrane-immobilized avidin and protein A can be applied to examine the epitopes of antigens...
  22. doi Analysis of trypsin inhibition activity in human plasma proteins after separation by non-denaturing two-dimensional electrophoresis
    Youji Shimazaki
    Graduate School of Science and Engineering Science Section and Venture Business Laboratory, Ehime University, Matsuyama, Japan Electronic address
    Clin Chim Acta 425:48-53. 2013
    ..The trypsin inhibition activities can then be examined following the separation and immobilization of the proteins under non-denaturing conditions...
  23. doi Successive analysis of antigen trapping and enzymatic digestion on membrane-immobilized avidin
    Youji Shimazaki
    Graduate School of Science and Engineering Science Section and Venture Business Laboratory, Ehime University, Matsuyama City, Japan
    Anal Biochem 422:55-7. 2012
    ..These results indicate that target substances can be specifically trapped and digested on membrane-immobilized avidin...
  24. doi Analysis of activity of esterase captured onto an immunoaffinity membrane
    Youji Shimazaki
    Graduate School of Science and Engineering Science Section, Ehime University, Matsuyama, Japan
    Clin Chim Acta 413:269-72. 2012
    ..The enzymatic activities of these captured proteins can then be monitored by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)...
  25. doi Analysis of enzyme activity regulation by non-denaturing electrophoresis and application of this regulation for enzyme reactor production
    Youji Shimazaki
    Department of Chemistry and Biology, Graduate School of Science and Engineering and Venture Business Laboratory, Ehime University, Matsuyama, Japan
    J Enzyme Inhib Med Chem 28:894-9. 2013
    ..These results indicate that non-denaturing electrophoresis can be applied to select enzymes that self-regulate their activities and subsequently aid in the development of enzyme reactors that can control the enzyme activities. ..
  26. doi Antigen digestion on the target plate of MALDI-TOF MS after isolation using an immunoaffinity membrane
    Youji Shimazaki
    Graduate School of Science and Engineering Science Section and Venture Business Laboratory, Ehime University, Matsuyama, Japan
    J Pharm Biomed Anal 83:293-8. 2013
    ..This approach would be particularly applicable to the analysis of the primary structure and the less stable and highly accessible regions of antigens from limited sample volumes...
  27. doi Enzymolysis of high density lipoprotein with a combination of membrane-immobilized esterase and trypsin
    Takahiro Sakikawa
    Graduate School of Science and Engineering Science Section and Venture Business Laboratory, Ehime University, Matsuyama 790 8577, Japan
    J Pharm Biomed Anal 71:179-82. 2012
    ..The data indicate that the lipid components of lipoproteins are released by membrane-immobilized esterase. This method can be used to investigate the structure and function of other apolipoproteins...
  28. doi Reversible inhibition of esterase activity after separation and immobilization
    Takahiro Sakikawa
    Graduate School of Science and Engineering, Ehime University, Matsuyama, 790 8577, Japan
    Appl Biochem Biotechnol 165:69-74. 2011
    ..Furthermore, this method can be developed to the production of an enzyme reactor able to regulate amounts of lipids...
  29. doi Direct trapping and analysis of hemoglobin in flowing fluid using membrane-immobilized anti-haptoglobin antibody
    Youji Shimazaki
    Graduate School of Science and Engineering Science Section and Venture Business Laboratory, Ehime University, Matsuyama, Japan
    J Pharm Biomed Anal 56:1085-8. 2011
    ..The results indicate that hemolysis can be examined by direct trapping and analysis of hemoglobin under physiological conditions...
  30. doi Retaining activity of enzymes after capture and extraction within a single-drop of biological fluid using immunoaffinity membranes
    Youji Shimazaki
    Department of Chemistry and Biology, Graduate School of Science and Engineering, Ehime University, Matsuyama, Japan Electronic address
    J Chromatogr B Analyt Technol Biomed Life Sci 1021:108-13. 2016
    ....
  31. doi Micro-scale extraction and analysis of intact carboxylesterase after trapping on an immunoaffinity membrane surface
    Ayaka Kimura
    Graduate School of Science and Engineering Science Section, Ehime University, Matsuyama, 790 8577, Japan
    Appl Biochem Biotechnol 172:4053-61. 2014
    ..These results indicate that micro-scale extraction and analysis of a carboxylesterase are possible when the enzyme is trapped using an immunoaffinity membrane and eluted with aspartic acid...