Yousuke Nishio

Summary

Affiliation: Ajinomoto Co
Country: Japan

Publications

  1. pmc Analysis of L-glutamic acid fermentation by using a dynamic metabolic simulation model of Escherichia coli
    Yousuke Nishio
    Institute for Innovation, Ajinomoto Co, Inc, Suzuki cho 1 1, Kawasaki ku, Kawasaki City, Kanagawa 210 8681, Japan
    BMC Syst Biol 7:92. 2013
  2. pmc Computer-aided rational design of the phosphotransferase system for enhanced glucose uptake in Escherichia coli
    Yousuke Nishio
    Fermentation and Biotechnology Laboratories, Ajinomoto Co Inc, Kawasaki, Japan
    Mol Syst Biol 4:160. 2008
  3. ncbi request reprint Evolutionary process of amino acid biosynthesis in Corynebacterium at the whole genome level
    Yousuke Nishio
    Institute of Life Sciences, Ajinomoto Co, Inc, Kawasaki, Japan
    Mol Biol Evol 21:1683-91. 2004
  4. pmc Impact of an energy-conserving strategy on succinate production under weak acidic and anaerobic conditions in Enterobacter aerogenes
    Yoshinori Tajima
    Institute for Innovation, Ajinomoto Co, Inc, 1 1 Suzuki cho, Kawasaki ku, Kawasaki, 210 8681, Japan
    Microb Cell Fact 14:80. 2015
  5. pmc Effects of eliminating pyruvate node pathways and of coexpression of heterogeneous carboxylation enzymes on succinate production by Enterobacter aerogenes
    Yoshinori Tajima
    Institute for Innovation, Ajinomoto Co, Inc, Kawasaki ku, Kawasaki, Japan
    Appl Environ Microbiol 81:929-37. 2015
  6. doi request reprint Study of the role of anaerobic metabolism in succinate production by Enterobacter aerogenes
    Yoshinori Tajima
    Institute for Innovation, Ajinomoto Co, Inc, 1 1 Suzuki cho, Kawasaki ku, Kawasaki, 210 8681, Japan
    Appl Microbiol Biotechnol 98:7803-13. 2014
  7. pmc Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens
    Yousuke Nishio
    Fermentation and Biotechnology Laboratories, Ajinomoto Co, Inc, Kawasaki, Kanagawa 210 8681, Japan
    Genome Res 13:1572-9. 2003
  8. ncbi request reprint The genome stability in Corynebacterium species due to lack of the recombinational repair system
    Yoji Nakamura
    Center for Information Biology and DNA Data Bank of Japan, National Institute of Genetics, Mishima, Japan
    Gene 317:149-55. 2003

Collaborators

Detail Information

Publications8

  1. pmc Analysis of L-glutamic acid fermentation by using a dynamic metabolic simulation model of Escherichia coli
    Yousuke Nishio
    Institute for Innovation, Ajinomoto Co, Inc, Suzuki cho 1 1, Kawasaki ku, Kawasaki City, Kanagawa 210 8681, Japan
    BMC Syst Biol 7:92. 2013
    ..However, to confirm the biological appropriateness of our model, sensitivity analysis and experimental validation were required...
  2. pmc Computer-aided rational design of the phosphotransferase system for enhanced glucose uptake in Escherichia coli
    Yousuke Nishio
    Fermentation and Biotechnology Laboratories, Ajinomoto Co Inc, Kawasaki, Japan
    Mol Syst Biol 4:160. 2008
    ..By using biological experiments, we validated the prediction and the presented strategy, thereby enhancing the specific glucose uptake rate...
  3. ncbi request reprint Evolutionary process of amino acid biosynthesis in Corynebacterium at the whole genome level
    Yousuke Nishio
    Institute of Life Sciences, Ajinomoto Co, Inc, Kawasaki, Japan
    Mol Biol Evol 21:1683-91. 2004
    ..Thus, we conclude that the evolutionary events of gene loss and horizontal gene transfer must have been responsible for functional differentiation in amino acid biosynthesis of the three species of Corynebacteria...
  4. pmc Impact of an energy-conserving strategy on succinate production under weak acidic and anaerobic conditions in Enterobacter aerogenes
    Yoshinori Tajima
    Institute for Innovation, Ajinomoto Co, Inc, 1 1 Suzuki cho, Kawasaki ku, Kawasaki, 210 8681, Japan
    Microb Cell Fact 14:80. 2015
    ..23 g/L/h. Although this demonstrates proof-of-concept that bacterium-based succinate fermentation can be improved under weakly acidic conditions, several parameters still required further optimization...
  5. pmc Effects of eliminating pyruvate node pathways and of coexpression of heterogeneous carboxylation enzymes on succinate production by Enterobacter aerogenes
    Yoshinori Tajima
    Institute for Innovation, Ajinomoto Co, Inc, Kawasaki ku, Kawasaki, Japan
    Appl Environ Microbiol 81:929-37. 2015
    ..These findings can be applied to identify additional engineering targets to increase succinate production. ..
  6. doi request reprint Study of the role of anaerobic metabolism in succinate production by Enterobacter aerogenes
    Yoshinori Tajima
    Institute for Innovation, Ajinomoto Co, Inc, 1 1 Suzuki cho, Kawasaki ku, Kawasaki, 210 8681, Japan
    Appl Microbiol Biotechnol 98:7803-13. 2014
    ..5% yield (grams of succinate produced per gram of glucose consumed) at pH <6.2 and anaerobic conditions. Thus, we showed the potential of bacteria-based succinate fermentation under weak acidic conditions...
  7. pmc Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens
    Yousuke Nishio
    Fermentation and Biotechnology Laboratories, Ajinomoto Co, Inc, Kawasaki, Kanagawa 210 8681, Japan
    Genome Res 13:1572-9. 2003
    ..Our results strongly suggest that the accumulation of these three types of amino acid substitutions correlates with the acquisition of thermostability and is responsible for the greater GC content of C. efficiens...
  8. ncbi request reprint The genome stability in Corynebacterium species due to lack of the recombinational repair system
    Yoji Nakamura
    Center for Information Biology and DNA Data Bank of Japan, National Institute of Genetics, Mishima, Japan
    Gene 317:149-55. 2003
    ..The genome stability in Corynebacterium species will give us hints of the speciation mechanism with the non-shuffled genome, particularly the importance of horizontal gene transfer and nucleotide substitution in the genome...