N Volpi

Summary

Affiliation: University of Modena and Reggio Emilia
Country: Italy

Publications

  1. ncbi request reprint Influence of charge density, sulfate group position and molecular mass on adsorption of chondroitin sulfate onto coral
    Nicola Volpi
    Department of Animal Biology, Biological Chemistry, University of Modena and Reggio Emilia, Italy
    Biomaterials 23:3015-22. 2002
  2. ncbi request reprint Chondroitin sulfate in normal human plasma is modified depending on the age. Its evaluation in patients with pseudoxanthoma elasticum
    Nicola Volpi
    Department of Biologia Animale University of Modena and Reggio Emilia Via Campi 213 D, 41100 Modena, Italy
    Clin Chim Acta 370:196-200. 2006
  3. ncbi request reprint Analysis of flavonoids from propolis by on-line HPLC-electrospray mass spectrometry
    Nicola Volpi
    Department of Biologia Animale, University of Modena and Reggio Emilia, Via Campi 213 D, 41100 Modena, Italy
    J Pharm Biomed Anal 42:354-61. 2006
  4. ncbi request reprint Hyaluronan in Medical Practice
    Katarina Valachova
    Department of Life Sciences, University of Modena and Reggio Emilia, Via Campi 213 D, 41100 Modena, Italy
    Curr Med Chem 23:3607-3617. 2016
  5. ncbi request reprint Chondroitin Sulfate and Glucosamine as Disease Modifying Anti- Osteoarthritis Dru gs (DMOADs)
    Veronica Mantovani
    Department of Life Sciences University of Modena and Reggio Emilia, via Campi 213 D 41100 Modena, Italy
    Curr Med Chem 23:1139-51. 2016
  6. doi request reprint Glycosaminoglycan blotting and detection after electrophoresis separation
    Nicola Volpi
    Department of Life Sciences, University of Modena and Reggio Emilia, Via Campi 213 D, 41100, Modena, Italy
    Methods Mol Biol 1312:119-27. 2015
  7. ncbi request reprint Condrosulf®: structural characterization, pharmacological activities and mechanism of action
    Nicola Volpi
    Department of Life Sciences University of Modena and Reggio Emilia Via Campi 213 D 41100 Modena, Italy
    Curr Med Chem 21:3949-61. 2014
  8. doi request reprint Plasmatic kinetics of dermatan sulfate during enzyme replacement therapy with iduronate-2-sulfatase in a mucopolysaccharidosis II patient
    Nicola Volpi
    Department of Life Sciences, University of Modena and Reggio Emilia, Via Campi 213 D, 41100, Modena, Italy
    Glycoconj J 30:727-32. 2013
  9. doi request reprint Plasmatic dermatan sulfate and chondroitin sulfate determination in mucopolysaccharidoses
    Nicola Volpi
    Department of Life Sciences, University of Modena and Reggio Emilia, Modena, Italy
    J Pharm Biomed Anal 85:40-5. 2013
  10. pmc Electrophoresis for the analysis of heparin purity and quality
    Nicola Volpi
    Department of Biology, University of Modena and Reggio Emilia, Italy
    Electrophoresis 33:1531-7. 2012

Collaborators

Detail Information

Publications77

  1. ncbi request reprint Influence of charge density, sulfate group position and molecular mass on adsorption of chondroitin sulfate onto coral
    Nicola Volpi
    Department of Animal Biology, Biological Chemistry, University of Modena and Reggio Emilia, Italy
    Biomaterials 23:3015-22. 2002
    ....
  2. ncbi request reprint Chondroitin sulfate in normal human plasma is modified depending on the age. Its evaluation in patients with pseudoxanthoma elasticum
    Nicola Volpi
    Department of Biologia Animale University of Modena and Reggio Emilia Via Campi 213 D, 41100 Modena, Italy
    Clin Chim Acta 370:196-200. 2006
    ..6%. Furthermore, the group of PXE-affected subjects ranging from 50 to 74 years (mean 58.6) showed a significant increase of 15.5% in comparison with the group matched for age (mean 56.9) of healthy volunteers...
  3. ncbi request reprint Analysis of flavonoids from propolis by on-line HPLC-electrospray mass spectrometry
    Nicola Volpi
    Department of Biologia Animale, University of Modena and Reggio Emilia, Via Campi 213 D, 41100 Modena, Italy
    J Pharm Biomed Anal 42:354-61. 2006
    ..On-line HPLC-ESI/MS analysis constitutes an alternative to obtain typical fingerprints of propolis and a reliable identification of a large number of propolis polyphenolic components...
  4. ncbi request reprint Hyaluronan in Medical Practice
    Katarina Valachova
    Department of Life Sciences, University of Modena and Reggio Emilia, Via Campi 213 D, 41100 Modena, Italy
    Curr Med Chem 23:3607-3617. 2016
    ..Because of greatly increased interest currently in hyaluronan, the multiple functions of the polymer are presented here, including medicine and industry, as well as recent progress in the formulation of hyaluronan-based materials...
  5. ncbi request reprint Chondroitin Sulfate and Glucosamine as Disease Modifying Anti- Osteoarthritis Dru gs (DMOADs)
    Veronica Mantovani
    Department of Life Sciences University of Modena and Reggio Emilia, via Campi 213 D 41100 Modena, Italy
    Curr Med Chem 23:1139-51. 2016
    ..This comprehensive review aims to clarify the role of these compounds in the therapeutic molecules/ drugs useful to patients affected by osteoarthritis. ..
  6. doi request reprint Glycosaminoglycan blotting and detection after electrophoresis separation
    Nicola Volpi
    Department of Life Sciences, University of Modena and Reggio Emilia, Via Campi 213 D, 41100, Modena, Italy
    Methods Mol Biol 1312:119-27. 2015
    ..1-0.5 μg after irreversible or reversible staining. The membranes may be stained with reversible staining and the same lanes used for immunological detection or other applications...
  7. ncbi request reprint Condrosulf®: structural characterization, pharmacological activities and mechanism of action
    Nicola Volpi
    Department of Life Sciences University of Modena and Reggio Emilia Via Campi 213 D 41100 Modena, Italy
    Curr Med Chem 21:3949-61. 2014
    ....
  8. doi request reprint Plasmatic kinetics of dermatan sulfate during enzyme replacement therapy with iduronate-2-sulfatase in a mucopolysaccharidosis II patient
    Nicola Volpi
    Department of Life Sciences, University of Modena and Reggio Emilia, Via Campi 213 D, 41100, Modena, Italy
    Glycoconj J 30:727-32. 2013
    ....
  9. doi request reprint Plasmatic dermatan sulfate and chondroitin sulfate determination in mucopolysaccharidoses
    Nicola Volpi
    Department of Life Sciences, University of Modena and Reggio Emilia, Modena, Italy
    J Pharm Biomed Anal 85:40-5. 2013
    ..The same trend was determined for the only patient affected by MPS VI. Plasmatic DS and CS analyzed by the present procedure may be a useful diagnostic and screening marker for various forms of MPS...
  10. pmc Electrophoresis for the analysis of heparin purity and quality
    Nicola Volpi
    Department of Biology, University of Modena and Reggio Emilia, Italy
    Electrophoresis 33:1531-7. 2012
    ....
  11. doi request reprint Agarose-gel electrophoresis for the quality assurance and purity of heparin formulations
    Nicola Volpi
    Department of Biology, University of Modena and Reggio Emilia, Via Campi 213 D, 41100 Modena, Italy
    J Pharm Biomed Anal 67:144-7. 2012
    ....
  12. ncbi request reprint Advances in chondroitin sulfate analysis: application in physiological and pathological States of connective tissue and during pharmacological treatment of osteoarthritis
    Nicola Volpi
    Department of Biologia Animale, University of Modena and Reggio Emilia, Italy
    Curr Pharm Des 12:639-58. 2006
    ..Moreover, a critical evaluation of the biological relevance of the results obtained by the developed methodology is discussed...
  13. ncbi request reprint Electrophoretic approaches to the analysis of complex polysaccharides
    Nicola Volpi
    Department of Biologia Animale, Biological Chemistry Section, University of Modena and Reggio Emilia, Via Campi 213 D, 41100 Modena, Italy
    J Chromatogr B Analyt Technol Biomed Life Sci 834:1-13. 2006
    ..Moreover, a critical evaluation of the biological relevance of the results obtained by these electrophoresis approaches is presented...
  14. ncbi request reprint Glycosaminoglycan composition of the large freshwater mollusc bivalve Anodonta anodonta
    Nicola Volpi
    Dipartimento di Biologia Animale, University of Modena and Reggio Emilia, Modena, Italy
    Biomacromolecules 6:3174-80. 2005
    ..However, the anticoagulant activity of mollusc heparin was quite similar to that of pharmaceutical grade heparin. The data obtained again emphasize the heterogeneity of GAGs from molluscs...
  15. ncbi request reprint Separation of keratan-sulfate-derived disaccharides by high-performance liquid chromatography and postcolumn derivatization with 2-cyanoacetamide and fluorimetric detection
    Nicola Volpi
    Department of Biologia Animale, University of Modena and Reggio Emilia, 41100 Modena, Italy
    Anal Biochem 342:200-5. 2005
    ....
  16. ncbi request reprint Microdetermination of chondroitin sulfate in normal human plasma by fluorophore-assisted carbohydrate electrophoresis (FACE)
    Nicola Volpi
    Department of Biologia Animale, University of Modena and Reggio Emilia, Via Campi 213 D, 41100 Modena, Italy
    Clin Chim Acta 356:125-33. 2005
    ..This method was applied to the characterization of CS in 33 healthy human subjects ageing from 30 to 63 years old...
  17. doi request reprint Anti-inflammatory activity of chondroitin sulphate: new functions from an old natural macromolecule
    Nicola Volpi
    Department of Biology, University of Modena and Reggio Emilia, Via Campi 213 D, 41100 Modena, Italy
    Inflammopharmacology 19:299-306. 2011
    ....
  18. ncbi request reprint Therapeutic applications of glycosaminoglycans
    Nicola Volpi
    Department of Biologia Animale, Biological Chemistry Section, University of Modena and Reggio Emilia, Italy
    Curr Med Chem 13:1799-810. 2006
    ....
  19. doi request reprint Quality of different chondroitin sulfate preparations in relation to their therapeutic activity
    Nicola Volpi
    Department of Biologia Animale, Biological Chemistry Section, University of Modena and Reggio Emilia, Modena, Italy
    J Pharm Pharmacol 61:1271-80. 2009
    ..We aim to review the quality control of chondroitin sulfate in pharmaceutical-grade preparations and nutraceuticals...
  20. pmc High-performance liquid chromatography-mass spectrometry for mapping and sequencing glycosaminoglycan-derived oligosaccharides
    Nicola Volpi
    Department of Biology, University of Modena and Reggio Emilia, Modena, Italy
    Nat Protoc 5:993-1004. 2010
    ..The preparation of oligosaccharides can be carried out in 10 h, with subsequent HPLC analysis in 1-2 h and HPLC-MS analysis taking another 2 h...
  21. ncbi request reprint Purification of the Escherichia coli K5 capsular polysaccharide and use of high-performance capillary electrophoresis to qualitative and quantitative monitor the process
    Nicola Volpi
    Department of Biologia Animale, University of Modena and Reggio Emilia, Modena, Italy
    Electrophoresis 25:3307-12. 2004
    ..17,000, also confirmed by high-performance size-exclusion chromatography analysis, yielding approx. 17 200...
  22. ncbi request reprint On-line HPLC/ESI-MS separation and characterization of hyaluronan oligosaccharides from 2-mers to 40-mers
    Nicola Volpi
    Department of Biologia Animale, University of Modena and Reggio Emilia, Modena, Italy
    Anal Chem 79:6390-7. 2007
    ....
  23. ncbi request reprint Analytical aspects of pharmaceutical grade chondroitin sulfates
    Nicola Volpi
    Department of Biologia Animale, Biological Chemistry Section, University of Modena and Reggio Emilia, Italy
    J Pharm Sci 96:3168-80. 2007
    ..Finally, the necessity for reference standards having high specificity, purity and well-known physico-chemical properties useful for accurate and reproducible quantitative analyses will be discussed...
  24. ncbi request reprint Chondroitin C lyase [4.2.2.] is unable to cleave fructosylated sequences inside the partially fructosylated Escherichia coli K4 polymer
    Nicola Volpi
    Department of Biologia Animale, University of Modena and Reggio Emilia, Modena, Italy
    Glycoconj J 25:451-7. 2008
    ..This novel specific activity of chondroitinase C was also useful for the production of discrete microgram amounts of fully fructosylated oligomers, from 4- to 10-mers, from E. coli K4 for possible further studies and applications...
  25. ncbi request reprint Mass spectrometry characterization of Escherichia coli K4 oligosaccharides from 2-mers to more than 20-mers
    Nicola Volpi
    Department of Biologia Animale, University of Modena and Reggio Emilia, Modena, Italy
    Rapid Commun Mass Spectrom 21:3459-66. 2007
    ....
  26. pmc Capillary electrophoresis of complex natural polysaccharides
    Nicola Volpi
    Department of Biologia Animale, Biological Chemistry Section, University of Modena and Reggio Emilia, Modena, Italy
    Electrophoresis 29:3095-106. 2008
    ..In this review, novel off-line and on-line CE-MS and MS/MS methods for screening of GAG-derived oligosaccharides and disaccharides will be discussed...
  27. doi request reprint High-performance liquid chromatography and on-line mass spectrometry detection for the analysis of chondroitin sulfates/hyaluronan disaccharides derivatized with 2-aminoacridone
    Nicola Volpi
    Department of Biologia Animale, University of Modena and Reggio Emilia, 41100 Modena, Italy
    Anal Biochem 397:12-23. 2010
    ..Furthermore, purified endogenous normal human plasma CS disaccharides were also evaluated by means of RP-HPLC-(Fp)-ESI-MS...
  28. doi request reprint Micellar electrokinetic capillary chromatography determination of alginic acid in pharmaceutical formulations after treatment with alginate lyase and UV detection
    Nicola Volpi
    Department of Biologia Animale, University of Modena and Reggio Emilia, Modena, Italy
    Electrophoresis 29:3504-10. 2008
    ..The quantitative results obtained were 101.2+/-3.4% of AA content in tablets and 98.4+/-2.8% in liquid formulation, in total conformity with the label claims...
  29. ncbi request reprint Role, metabolism, chemical modifications and applications of hyaluronan
    Nicola Volpi
    Department of Biologia Animale, University of Modena and Reggio Emilia, Via Campi 213 D, I 41100 Modena, Italy
    Curr Med Chem 16:1718-45. 2009
    ..Recent advances in biotechnological approaches for the preparation of HA-based materials, and as a component of tissue scaffolding for artificial organs will also be presented...
  30. pmc Mass spectrometry for the characterization of unsulfated chondroitin oligosaccharides from 2-mers to 16-mers. Comparison with hyaluronic acid oligomers
    Nicola Volpi
    Department of Biologia Animale, University of Modena and Reggio Emilia, Modena, Italy
    Rapid Commun Mass Spectrom 22:3526-30. 2008
    ..Thus, under the experimental conditions used in the current study, we were unable to distinguish between unsulfated chondroitin and HA...
  31. doi request reprint Capillary blotting of glycosaminoglycans on nitrocellulose membranes after agarose-gel electrophoresis separation
    Nicola Volpi
    Department of Biologia Animale, University of Modena and Reggio Emilia, Via Campi 213 D, 41100, Modena, Italy
    Methods Mol Biol 536:95-102. 2009
    ..1-0.5 microg after irreversible or reversible staining. The membranes may be stained with reversible staining and the same lanes are used for immunological detection or other applications...
  32. doi request reprint Structural characterization and antithrombin activity of dermatan sulfate purified from marine clam Scapharca inaequivalvis
    Nicola Volpi
    Department of Biologia Animale, University of Modena and Reggio Emilia, 41100 Modena, Italy
    Glycobiology 19:356-67. 2009
    ..Finally, S. inaequivalvis DS was calculated to possess a high heparin cofactor II activity of 169.2 +/- 10.7% fairly similar to that of several DS samples purified from porcine and bovine tissues...
  33. pmc Quantitative capillary electrophoresis determination of oversulfated chondroitin sulfate as a contaminant in heparin preparations
    Nicola Volpi
    Department of Biologia Animale, Section of Biochemistry, University of Modena and Reggio Emilia, 41100 Modena, Italy
    Anal Biochem 388:140-5. 2009
    ..Finally, a contaminated raw Hep sample was found to contain 38.9% OSCS, whereas a formulated contaminated Hep was calculated to have 39.7% OSCS...
  34. doi request reprint Capillary electrophoresis determination of glucosamine in nutraceutical formulations after labeling with anthranilic acid and UV detection
    Nicola Volpi
    Department of Biologia Animale, University of Modena and Reggio Emilia, Via Campi 213 D, 41100 Modena, Italy
    J Pharm Biomed Anal 49:868-71. 2009
    ..e. chondroitin sulfate, vitamin C and/or methylsulfonylmethane (MSM) as well as salts and other agents. The quantitative results obtained were in total conformity with the label claims...
  35. ncbi request reprint Application of high-performance capillary electrophoresis to the purification process of Escherichia coli K4 polysaccharide
    Nicola Volpi
    Department of Biologia Animale, University of Modena and Reggio Emilia, Via Campi 213 D, 41100 Modena, Italy
    J Chromatogr B Analyt Technol Biomed Life Sci 811:253-6. 2004
    ..Furthermore, HPCE is able to verify that the extraction and purification process adopted is not carried out under drastic conditions capable of inducing fructose removal from the polysaccharide backbone...
  36. ncbi request reprint Inhibition of human leukocyte elastase activity by chondroitin sulfates
    N Volpi
    Department of Biologia Animale, University of Modena, Italy
    Chem Biol Interact 105:157-67. 1997
    ..In particular, the inhibition of the enzymatic activity decreased with the percentage of non-sulfated disaccharide and increased with the amount of disaccharide-2,6-disulfated...
  37. ncbi request reprint Structural and functional modifications of bovine trypsin by heparins. Influence of heparin molecular mass and structure
    N Volpi
    Department of Biologia Animale, University of Modena, Italy
    Biochim Biophys Acta 1336:455-64. 1997
    ..The esterolytic activity of trypsin was inhibited to the same extent by heparin derivatives of various structure and charge density while activity undermet minor changes in the presence of heparin fractions of Mr lower than 4000...
  38. ncbi request reprint Characterization of a small chondroitin sulfate proteoglycan isolated from the mucus surrounding the embryos of Viviparus ater (Mollusca Gastropoda)
    N Volpi
    Department of Biologia Animale, Department of Biological Chemistry Section, via Berengario 14, University of Modena, 41100 Modena, Italy
    Biochim Biophys Acta 1380:239-48. 1998
    ....
  39. ncbi request reprint Influence of chondroitin sulfate charge density, sulfate group position, and molecular mass on Cu2+-mediated oxidation of human low-density lipoproteins: effect of normal human plasma-derived chondroitin sulfate
    N Volpi
    Department of Biologia Animale, Biological Chemistry Section, General Pathology Section, University of Modena, Modena, 41100, Italy
    J Biochem 125:297-304. 1999
    ..The protective capacity as to Cu2+-mediated LDL oxidation of CS is discussed in relation to its structure, also considering the physiological role of plasma CS in relation to factors that can alter its properties...
  40. ncbi request reprint Characterization of a low-sulfated chondroitin sulfate from the body of Viviparus ater (mollusca gastropoda). Modification of its structure by lead pollution
    N Volpi
    Department of Biologia Animale, University of Modena, Italy
    Glycoconj J 15:1071-8. 1998
    ..Simultaneously, the amount of unsaturated non-sulfated disaccharide increased with an overall decrease of the charge density...
  41. ncbi request reprint Stability studies of chondroitin sulfate
    N Volpi
    Department of Animal Biology, University of Modena, Italy
    Carbohydr Res 315:345-9. 1999
    ..Little or no beta-elimination occurs. A loss of O-sulfate groups was detected, producing desulfated derivatives...
  42. ncbi request reprint Adsorption of glycosaminoglycans onto coral--a new possible implant biomaterials for regeneration therapy
    N Volpi
    Department of Biologia Animale, University of Modena, Italy
    Biomaterials 20:1359-63. 1999
    ..Due to these peculiar properties, the combination of granules of natural coral with glycosaminoglycans makes this material potentially useful in osseointegration in bone metabolism or periodontal therapy...
  43. ncbi request reprint Oral bioavailability of chondroitin sulfate (Condrosulf) and its constituents in healthy male volunteers
    N Volpi
    Department of Biologia Animale, Biological Chemistry Section, University of Modena and Reggio Emilia, Italy
    Osteoarthritis Cartilage 10:768-77. 2002
    ..The possible physiological regulation of plasma levels of endogenous chondroitin sulfate during the day was also assessed...
  44. ncbi request reprint Detection of submicrogram quantities of glycosaminoglycans on agarose gels by sequential staining with toluidine blue and Stains-All
    Nicola Volpi
    Department of Animal Biology, University of Modena and Reggio Emilia, Modena, Italy
    Electrophoresis 23:4060-6. 2002
    ..This procedure may be particularly valuable in situations where the availability of glycosaminoglycan is very limited...
  45. ncbi request reprint Separation of capsular polysaccharide K4 and defructosylated K4 derived disaccharides by high-performance capillary electrophoresis and high-performance liquid chromatography
    Nicola Volpi
    Dipartimento di Biologia Animale, University of Modena and Reggio Emilia, Modena, Italy
    Electrophoresis 24:1063-8. 2003
    ..The described methods were used to evaluate the defructosylation process of K4 under drastic acid conditions. Good correspondence was found for the amount of unsaturated disaccharides for the two techniques...
  46. ncbi request reprint Milligram-scale preparation and purification of oligosaccharides of defined length possessing the structure of chondroitin from defructosylated capsular polysaccharide K4
    Nicola Volpi
    Department of Biologia Animale, University of Modena and Reggio Emilia, Via Campi 213 D, 41100 Modena, Italy
    Glycobiology 13:635-40. 2003
    ....
  47. ncbi request reprint Oral absorption and bioavailability of ichthyic origin chondroitin sulfate in healthy male volunteers
    N Volpi
    Department of Biologia Animale, Biological Chemistry Section, University of Modena and Reggio Emilia, Modena, Italy
    Osteoarthritis Cartilage 11:433-41. 2003
    ..The aim of this study was to assess the absorption of CS of ichthyic origin after oral administration to 20 healthy male volunteers...
  48. ncbi request reprint Purification and characterization of hyaluronic acid from the mollusc bivalve Mytilus galloprovincialis
    Nicola Volpi
    Department of Biologia Animale, University of Modena and Reggio Emilia, Via Campi 213 D, 41100 Modena, Italy
    Biochimie 85:619-25. 2003
    ..HA of M. galloprovincialis could have a physiological role in the regulation of cell functions...
  49. ncbi request reprint Separation of Escherichia coli 055:B5 lipopolysaccharide and detoxified lipopolysaccharide by high-performance capillary electrophoresis
    Nicola Volpi
    Department of Biologia Animale, University of Modena and Reggio Emilia, Modena, Italy
    Electrophoresis 24:3097-103. 2003
    ....
  50. ncbi request reprint Separation of capsular polysaccharide K4 and defructosylated K4 by high-performance capillary electrophoresis
    Nicola Volpi
    Department of Biologia Animale, University of Modena and Reggio Emilia, Modena, Italy
    Electrophoresis 25:692-6. 2004
    ..5 microg/microL) to 210 ng (3.5 microg/microL). The described method was used to evaluate the defructosylation process of K4 under drastic acid conditions...
  51. ncbi request reprint The pathobiology of osteoarthritis and the rationale for using the chondroitin sulfate for its treatment
    Nicola Volpi
    Department of Biologia Animale, Biological Chemistry Section, University of Modena and Reggio Emilia, Italy
    Curr Drug Targets Immune Endocr Metabol Disord 4:119-27. 2004
    ..These properties are also related to the oral adsorption of this molecule as high-molecular mass compounds having clusters of sulfate groups and high charge density capable of exert their chondroprotective activity in vivo...
  52. ncbi request reprint Separation of capsular polysaccharide-K4- and defructosylated-K4-derived disaccharides by high-performance liquid chromatography and postcolumn derivatization with 2-cyanoacetamide and fluorimetric detection
    Nicola Volpi
    Department of Biologia Animale, University of Modena and Reggio Emilia, 41100 Modena, Italy
    Anal Biochem 330:359-61. 2004
  53. ncbi request reprint Separation of flavonoids and phenolic acids from propolis by capillary zone electrophoresis
    Nicola Volpi
    Department of Biologia Animale, University of Modena and Reggio Emilia, Modena, Italy
    Electrophoresis 25:1872-8. 2004
    ....
  54. ncbi request reprint Simultaneous detection of submicrogram quantities of hyaluronic acid and dermatan sulfate on agarose-gel by sequential staining with toluidine blue and Stains-All
    Nicola Volpi
    Department of Biologia Animale, University of Modena and Reggio Emilia, Via Campi 213 D, 41100 Modena, Italy
    J Chromatogr B Analyt Technol Biomed Life Sci 820:131-5. 2005
    ....
  55. ncbi request reprint Activity of chondroitin ABC lyase on dermatan sulfate partially degraded by cupric-ion-mediated free-radical treatment
    N Volpi
    Department of Biologia Animale, University of Modena, Italy
    J Chromatogr B Biomed Appl 669:197-205. 1995
    ..This was confirmed by polyacrylamide gel electrophoresis and the time curves of enzymatic treatments evaluated by spectrophotometry...
  56. ncbi request reprint Activity of chondroitin ABC lyase and hyaluronidase on free-radical degraded chondroitin sulfate
    N Volpi
    Department of Biologia Animale, University of Modena, Italy
    Carbohydr Res 279:193-200. 1995
    ..This was confirmed by polyacrylamide-gel electrophoresis and by the time-courses of enzymatic treatment evaluated by spectrophotometric technique (for treatment with chondroitin ABC lyase)...
  57. ncbi request reprint Qualitative and quantitative studies of heparin and chondroitin sulfates in normal human plasma
    N Volpi
    Department of Biologia Animale, University of Modena, Italy
    Biochim Biophys Acta 1243:49-58. 1995
    ..This is discussed in light of other authors that described a polysaccharide molecule named heparan sulfate in human plasma...
  58. doi request reprint Determination of molecular mass values of chondroitin sulfates by fluorophore-assisted carbohydrate electrophoresis (FACE)
    Dania Buzzega
    Department of Biologia Animale, University of Modena and Reggio Emilia, Via Campi 213 D, 41100 Modena, Italy
    J Pharm Biomed Anal 51:969-72. 2010
    ....
  59. doi request reprint Fluorophore-assisted carbohydrate electrophoresis for the determination of molecular mass of heparins and low-molecular-weight (LMW) heparins
    Dania Buzzega
    Department of Biologia Animale, University of Modena and Reggio Emilia, Modena, Italy
    Electrophoresis 29:4192-202. 2008
    ....
  60. ncbi request reprint beta-Amyloid fibrillation and/or hyperhomocysteinemia modify striatal patterns of hyaluronic acid and dermatan sulfate: Possible role in the pathogenesis of Alzheimer's disease
    S Genedani
    Department of Biomedical Sciences, University of Modena and Reggio Emilia, Italy
    Curr Alzheimer Res 7:150-7. 2010
    ..However, the consequent modification of the ECM network might impair the extracellular diffusion pathways of different signal molecules and participate in the progression of AD...
  61. doi request reprint Serum IgG responses to food antigens in the italian population evaluated by highly sensitive and specific ELISA test
    Nicola Volpi
    Department of Biologia Animale, Biological Chemistry Section, University of Modena and Reggio Emilia, Modena, Italy
    J Immunoassay Immunochem 30:51-69. 2009
    ....
  62. ncbi request reprint Glycosaminoglycan blotting on nitrocellulose membranes treated with cetylpyridinium chloride after agarose-gel electrophoretic separation
    Francesca Maccari
    Department of Biologia Animale, University of Modena and Reggio Emilia, Modena, Italy
    Electrophoresis 23:3270-7. 2002
    ..Single bands were released from the membrane with an efficiency of 70-100% for further biochemical characterization...
  63. ncbi request reprint Anomalous structure of urinary glycosaminoglycans in patients with pseudoxanthoma elasticum
    Francesca Maccari
    Department of Biologia Animale, University of Modena and Reggio Emilia, Modena, Italy
    Clin Chem 49:380-8. 2003
    ..We investigated whether altered proteoglycan metabolism occurs in vivo and may be reflected in the urine of PXE individuals by analyzing the excreted polysaccharides...
  64. doi request reprint Structural characterization of chondroitin sulfate from sturgeon bone
    Francesca Maccari
    Department of Biology, University of Modena and Reggio Emilia, Via Campi 213 D, Modena, Italy
    Carbohydr Res 345:1575-80. 2010
    ..Finally, this newly identified source of CS would enable the production of this macromolecule having a particular repeating disaccharide composition, structure, and biological properties...
  65. ncbi request reprint The genome of the lepidopteran Mamestra brassicae has a vertebrate-like content of methyl-cytosine
    Mauro Mandrioli
    Dipartimento di Biologia Animale, Universita di Modena e Reggio Emilia, Via Campi 213 D, 41100 Modena, Italy
    Genetica 119:187-91. 2003
    ..Comparison of the restriction pattern of MspI and HpaII after hybridisation with the hobo, mariner, 28S and 5S rDNA probes did not evidence any difference indicating the absence of CpG methylation in all the studied repeated DNAs...
  66. ncbi request reprint Isolation and characterization of a heparin with high anticoagulant activity from the clam Tapes phylippinarum: evidence for the presence of a high content of antithrombin III binding site
    Marina Cesaretti
    Department of Biologia Animale, University of Modena and Reggio Emilia, Via Campi 213 D, 41100 Modena, Italy
    Glycobiology 14:1275-84. 2004
    ....
  67. doi request reprint Comparison of cetylpyridinium chloride and cetyltrimethylammonium bromide extractive procedures for quantification and characterization of human urinary glycosaminoglycans
    Dania Buzzega
    Department of Biology, University of Modena and Reggio Emilia, Modena, Italy
    Clin Chem Lab Med 48:1133-9. 2010
    ..Cetylpyridinium chloride (CPC) and cetyltrimethylammonium bromide (CETAB) are generally used to extract urinary GAGs prior to analysis. In this study, we evaluated the extraction of human urinary GAGs using CPC in comparison with CETAB...
  68. ncbi request reprint Direct and specific recognition of glycosaminoglycans by antibodies after their separation by agarose gel electrophoresis and blotting on cetylpyridinium chloride-treated nitrocellulose membranes
    Francesca Maccari
    Department of Animal Biology, University of Modena and Reggio Emilia, Modena, Italy
    Electrophoresis 24:1347-52. 2003
    ..Furthermore, immunodetection of heparan sulfate was performed using the anti-heparan sulfate antibody...
  69. ncbi request reprint Detection of submicrogram quantities of Escherichia coli lipopolysaccharides by agarose-gel electrophoresis
    Francesca Maccari
    Department of Biologia Animale, University of Modena and Reggio Emilia, 41100 Modena, Italy
    Anal Biochem 322:185-9. 2003
    ..The carbohydrate neutral moiety obtained from the hydrolysate does not migrate during electrophoresis. This was utilized to monitor quantitatively the removal process of the Lipid A component for the formation of the detoxified LPS...
  70. doi request reprint Structural characterization of the skin glycosaminoglycans in patients with pseudoxanthoma elasticum
    Francesca Maccari
    Department of Animal Biology, University of Modena and Reggio Emilia, Modena, Italy
    Int J Dermatol 47:1024-7. 2008
    ..Complex polysaccharides, glycosaminoglycans (GAGs), their amount, and fine structure were determined in the skin (epidermis + dermis) of pseudoxanthoma elasticum (PXE)-affected patients in comparison with healthy subjects...
  71. doi request reprint Glycosaminoglycan levels and structure in a mucopolysaccharidosis IIIA mice and the effect of a highly secreted sulfamidase engineered to cross the blood-brain barrier
    F Maccari
    Department of Life Sciences, University of Modena and Reggio Emilia, Via Campi 213 D, 41125, Modena, Italy
    Metab Brain Dis 32:203-210. 2017
    ....
  72. ncbi request reprint Purification and characterization of heparin from the Italian clam Callista chione
    Elisa Luppi
    Department of Biologia Animale, University of Modena and Reggio Emilia, Via Campi 213 D, 41100 Modena, Italy
    Biomacromolecules 6:1672-8. 2005
    ..As a consequence, Callista chione heparin is a low-sulfated polysaccharide showing a specific decrease of the sulfatation in position 2 of the uronic acid units...
  73. doi request reprint Shockwave lithotripsy and protective role of inosine: early and late evaluation in an experimental model
    Stefano De Stefani
    Department of Urology, University of Modena and Reggio Emilia, Modena, Italy
    J Endourol 22:1059-63. 2008
    ..The aim of our experimental study is to assess the efficacy of inosine in avoidance of acute renal damage after SWL...
  74. ncbi request reprint Composition of urinary glycosaminoglycans in a patient with pseudoxanthoma elasticum and familial Mediterranean fever
    Nicola Volpi
    Clin Chim Acta 359:207-9. 2005
  75. ncbi request reprint Fine characterization of mitral valve glycosaminoglycans and their modification with degenerative disease
    Luca Dainese
    Department of Cardiac and Vascular Surgery, Centro Cardiologico Monzino, IRCCS University of Milan, Milan, Italy
    Clin Chem Lab Med 45:361-6. 2007
    ....
  76. ncbi request reprint Evaluation of chondroitin sulfate bioactivity in hippocampal neurones and the astrocyte cell line U373: influence of position of sulfate groups and charge density
    Alfred Rapp
    MedUniWien, Center of Physiology and Pathophysiology, Währingerstrasse 10, 1090 Vienna, Austria
    Basic Clin Pharmacol Toxicol 96:37-43. 2005
    ..Besides dosage, sulfation density and position are relevant for effects of chondroitin sulfate in neuronal regenerative activity...
  77. ncbi request reprint Mobilization of osmotically inactive Na+ by growth and by dietary salt restriction in rats
    Markus Schafflhuber
    Department of Nephrology and Hypertension, Friedrich Alexander University, Erlangen, Nurnberg, Germany
    Am J Physiol Renal Physiol 292:F1490-500. 2007
    ....