M Spinaci

Summary

Affiliation: University of Bologna
Country: Italy

Publications

  1. ncbi request reprint Sperm sorting procedure induces a redistribution of Hsp70 but not Hsp60 and Hsp90 in boar spermatozoa
    Marcella Spinaci
    Dipartimento di Morfofisiologia Veterinaria e Produzioni Animali, Facolta di Medicina Veterinaria, Universita di Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia, Italia
    J Androl 27:899-907. 2006
  2. ncbi request reprint Paternal chromatin remodelling in mouse oocytes following fertilization
    M Spinaci
    Department of Veterinary Morphophysiology and Animal Production, University of Bologna, Italy
    Vet Res Commun 27:241-3. 2003
  3. ncbi request reprint In vitro production of cat blastocysts of predetermined sex using flow cytometrically sorted semen
    Marcella Spinaci
    Department of Veterinary Morphophysiology and Animal Production DIMORFIPA, University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia BO, Italy
    Theriogenology 67:872-7. 2007
  4. ncbi request reprint Maternal chromatin remodeling during maturation and after fertilization in mouse oocytes
    Marcella Spinaci
    Dipartimento di Morfofisiologia Veterinaria e Produzioni Animali, Facolta di Medicina Veterinaria, Universita di Bologna, Ozzano Emilia, Italia
    Mol Reprod Dev 69:215-21. 2004
  5. ncbi request reprint Effect of staining and sorting on boar sperm membrane integrity, mitochondrial activity and in vitro blastocyst development
    M Spinaci
    Dipartimento di Morfofisiologia Veterinaria e Produzioni Animali, Facolta di Medicina Veterinaria, Universita di Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia, Italy
    Theriogenology 64:191-201. 2005
  6. ncbi request reprint Immunolocalization of heat shock protein 70 (Hsp 70) in boar spermatozoa and its role during fertilization
    Marcella Spinaci
    Dipartimento di Morfofisiologia Veterinaria e Produzioni Animali, Facolta di Medicina Veterinaria, Universita di Bologna, Ozzano Emilia, Italia
    Mol Reprod Dev 72:534-41. 2005
  7. ncbi request reprint Assessment of some morphofunctional characteristics of flow-cytometrically sorted and stained boar spermatozoa
    M Spinaci
    Department of Veterinary Morphophysiology and Animal Production, University of Bologna, Ozzano Emilia, Italy
    Vet Res Commun 29:207-9. 2005
  8. doi request reprint Effect of liquid storage on sorted boar spermatozoa
    M Spinaci
    DIMORFIPA, University of Bologna, Bologna, Italy
    Theriogenology 74:741-8. 2010
  9. doi request reprint Comparative immunolocalization of GLUTs 1, 2, 3 and 5 in boar, stallion and dog spermatozoa
    D Bucci
    Dipartimento di Morfofisiologia Veterinaria e Produzioni Animali DIMORFIPA, Universita di Bologna, Ozzano Emilia, Bologna, Italia
    Reprod Domest Anim 45:315-22. 2010
  10. doi request reprint Vitrification of pig oocytes induces changes in histone H4 acetylation and histone H3 lysine 9 methylation (H3K9)
    M Spinaci
    Dipartimento di Scienze Mediche Veterinarie DSMVet, Universita di Bologna, Via Tolara di Sopra, 50, 40064, Ozzano Emilia BO, Italy
    Vet Res Commun 36:165-71. 2012

Detail Information

Publications31

  1. ncbi request reprint Sperm sorting procedure induces a redistribution of Hsp70 but not Hsp60 and Hsp90 in boar spermatozoa
    Marcella Spinaci
    Dipartimento di Morfofisiologia Veterinaria e Produzioni Animali, Facolta di Medicina Veterinaria, Universita di Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia, Italia
    J Androl 27:899-907. 2006
    ....
  2. ncbi request reprint Paternal chromatin remodelling in mouse oocytes following fertilization
    M Spinaci
    Department of Veterinary Morphophysiology and Animal Production, University of Bologna, Italy
    Vet Res Commun 27:241-3. 2003
  3. ncbi request reprint In vitro production of cat blastocysts of predetermined sex using flow cytometrically sorted semen
    Marcella Spinaci
    Department of Veterinary Morphophysiology and Animal Production DIMORFIPA, University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia BO, Italy
    Theriogenology 67:872-7. 2007
    ..Our results show, for the first time, that X- and Y-chromosome bearing spermatozoa sorted by high-speed flow cytometry can be successfully used in an IVM-IVF system to obtain cat embryos of a predetermined sex...
  4. ncbi request reprint Maternal chromatin remodeling during maturation and after fertilization in mouse oocytes
    Marcella Spinaci
    Dipartimento di Morfofisiologia Veterinaria e Produzioni Animali, Facolta di Medicina Veterinaria, Universita di Bologna, Ozzano Emilia, Italia
    Mol Reprod Dev 69:215-21. 2004
    ..This manipulation of chromatin remodeling may have applications regarding the biological significance of aberrant DNA methylation...
  5. ncbi request reprint Effect of staining and sorting on boar sperm membrane integrity, mitochondrial activity and in vitro blastocyst development
    M Spinaci
    Dipartimento di Morfofisiologia Veterinaria e Produzioni Animali, Facolta di Medicina Veterinaria, Universita di Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia, Italy
    Theriogenology 64:191-201. 2005
    ..In conclusion, despite the damages induced by sorting procedures, semen sorted as fresh or after storage at 17 degrees C can be successfully used for in vitro production of pig embryos...
  6. ncbi request reprint Immunolocalization of heat shock protein 70 (Hsp 70) in boar spermatozoa and its role during fertilization
    Marcella Spinaci
    Dipartimento di Morfofisiologia Veterinaria e Produzioni Animali, Facolta di Medicina Veterinaria, Universita di Bologna, Ozzano Emilia, Italia
    Mol Reprod Dev 72:534-41. 2005
    ..The overall data demonstrate that Hsp70 is present on boar sperm with a dynamic redistribution as the sperm undergoes capacitation and acrosome reaction and suggest an important role of this protein during porcine gamete interaction...
  7. ncbi request reprint Assessment of some morphofunctional characteristics of flow-cytometrically sorted and stained boar spermatozoa
    M Spinaci
    Department of Veterinary Morphophysiology and Animal Production, University of Bologna, Ozzano Emilia, Italy
    Vet Res Commun 29:207-9. 2005
  8. doi request reprint Effect of liquid storage on sorted boar spermatozoa
    M Spinaci
    DIMORFIPA, University of Bologna, Bologna, Italy
    Theriogenology 74:741-8. 2010
    ..05) influenced by storage after sorting. In order to minimize damages that compromise fertility and function of sex-sorted boar spermatozoa, the mechanisms by which sorting and liquid storage cause these injures require further study...
  9. doi request reprint Comparative immunolocalization of GLUTs 1, 2, 3 and 5 in boar, stallion and dog spermatozoa
    D Bucci
    Dipartimento di Morfofisiologia Veterinaria e Produzioni Animali DIMORFIPA, Universita di Bologna, Ozzano Emilia, Bologna, Italia
    Reprod Domest Anim 45:315-22. 2010
    ..Only in dog spermatozoa changes in GLUTs distribution were demonstrated; these changes could be related to the different metabolic needs and modifications occurring in the sperm cell...
  10. doi request reprint Vitrification of pig oocytes induces changes in histone H4 acetylation and histone H3 lysine 9 methylation (H3K9)
    M Spinaci
    Dipartimento di Scienze Mediche Veterinarie DSMVet, Universita di Bologna, Via Tolara di Sopra, 50, 40064, Ozzano Emilia BO, Italy
    Vet Res Commun 36:165-71. 2012
    ....
  11. doi request reprint Comparative immunolocalization of heat shock proteins (Hsp)-60, -70, -90 in boar, stallion, dog and cat spermatozoa
    S Volpe
    Dipartimento di Morfofisiologia Veterinaria e Produzioni Animali DIMORFIPA, Universita di Bologna, Bologna, Italy
    Reprod Domest Anim 43:385-92. 2008
    ....
  12. doi request reprint Pig oocyte vitrification by Cryotop method and the activation of the apoptotic cascade
    C Vallorani
    Dipartimento di Scienze Mediche Veterinarie, Universita di Bologna, Ozzano Emilia, BO, Italy
    Anim Reprod Sci 135:68-74. 2012
    ..All these data confirm the involvement of apoptotic mechanisms on the injuries induced by vitrification procedure in pig oocytes; explanation of this phenomenon could be useful to improve oocytes' cryopreservation protocols...
  13. doi request reprint Quality and fertilizing ability in vivo of sex-sorted stallion spermatozoa
    G Mari
    Veterinary Clinical Department, University of Bologna, Bologna, Italy
    Reprod Domest Anim 45:331-5. 2010
    ..In conclusion, despite the reduction in sperm motility, sex sorting did not impair stallion sperm viability and mitochondrial activity immediately post-thaw; moreover, the sexed spermatozoa retained the ability to fertilize in vivo...
  14. doi request reprint Boar sperm changes after sorting and encapsulation in barium alginate membranes
    M Spinaci
    Department of Veterinary Medical Sciences DIMEVET, University of Bologna, Ozzano dell Emilia, Bologna, Italy
    Theriogenology 80:526-32. 2013
    ..These results suggest the possibility of a future utilization of the encapsulation technology in order to store sorted spermatozoa and permit their controlled release in the female genital tract...
  15. doi request reprint Pig oocyte vitrification by cryotop method: effects on viability, spindle and chromosome configuration and in vitro fertilization
    G Galeati
    Dipartimento di Scienze Mediche Veterinarie, Universita di Bologna, Ozzano Emilia, Italy
    Anim Reprod Sci 127:43-9. 2011
    ..Further improvements of the current protocol will be necessary in order to optimize the Cryotop method for vitrifying pig matured oocytes...
  16. doi request reprint Effect of sex sorting on CTC staining, actin cytoskeleton and tyrosine phosphorylation in bull and boar spermatozoa
    D Bucci
    Dipartimento di Scienze Mediche Veterinarie, Universita di Bologna, Via Tolara di Sopra, 50, 40064 Ozzano dell Emilia, Bologna, Italy
    Theriogenology 77:1206-16. 2012
    ....
  17. doi request reprint Sperm-mediated gene transfer-treated spermatozoa maintain good quality parameters and in vitro fertilization ability in swine
    M L Bacci
    Department of Veterinary Morphophysiology and Animal Production DIMORFIPA, University of Bologna, Bologna, Italy
    Theriogenology 72:1163-70. 2009
    ....
  18. doi request reprint Effects of epigallocatechin-3-gallate (EGCG) on in vitro maturation and fertilization of porcine oocytes
    M Spinaci
    DIMORFIPA, University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia, Bologna, Italy
    Theriogenology 69:877-85. 2008
    ....
  19. doi request reprint Detection and localization of GLUTs 1, 2, 3 and 5 in donkey spermatozoa
    D Bucci
    Dipartimento di Morfofisiologia Veterinaria e Produzioni Animali, Universita di Bologna, Ozzano Emilia, Bologna, Italy
    Reprod Domest Anim 45:e217-20. 2010
    ..We also highlighted some differences between GLUTs distribution and molecular weight in donkey spermatozoa and its nearest relative, the horse...
  20. doi request reprint Effects of antioxidants on boar spermatozoa during sorting and storage
    C Vallorani
    DIMORFIPA, University of Bologna, 40064 Ozzano Emilia, Bologna, Italy
    Anim Reprod Sci 122:58-65. 2010
    ....
  21. ncbi request reprint Viability and DNA fragmentation in differently sorted boar spermatozoa
    M De Ambrogi
    Dipartimento di Morfofisiologia Veterinaria e Produzioni Animali, Facolta di Medicina Veterinaria, Universita di Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia BO, Italia
    Theriogenology 66:1994-2000. 2006
    ..In conclusion, we may believe that sex-sorting procedure as a whole does not affect either viability or DFI and that shear mechanical forces are a relevant source of DNA damage for sorted semen...
  22. doi request reprint Beta-mercaptoethanol supplementation of in vitro maturation medium does not influence nuclear and cytoplasmic maturation of equine oocytes
    B Merlo
    Department of Veterinary Medical Sciences, University of Bologna, Bologna, Ozzano Emilia, Italy
    Reprod Domest Anim 51:992-996. 2016
    ..6% vs 38.5% and 41.3%, respectively). In conclusion, the addition of BME at 0.1 and 0.7 mM to the maturation medium, in our culture conditions, has no effect on nuclear and cytoplasmic maturation of equine oocytes...
  23. ncbi request reprint Embelin supplementation of in vitro maturation medium does not influence nuclear and cytoplasmic maturation of pig oocytes
    G Galeati
    Dipartimento di Scienze Mediche Veterinarie DIMEVET, Universita di Bologna, Bologna, Italia
    J Physiol Pharmacol 67:513-519. 2016
    ....
  24. ncbi request reprint Stimulatory effects of fasting on vascular endothelial growth factor (VEGF) production by growing pig ovarian follicles
    G Galeati
    Dipartimento di Morfofisiologia Veterinaria e Produzioni Animali, Universita di Bologna, Via Tolara di Sopra, 50, 40064 Ozzano Emilia BO, Italy
    Reproduction 126:647-52. 2003
    ....
  25. ncbi request reprint Gastric immunolocalization and plasma profiles of acyl-ghrelin in fasted and fasted-refed prepuberal gilts
    N Govoni
    Department of Veterinary Morphophysiology and Animal Production, University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia BO, Italy
    J Endocrinol 186:505-13. 2005
    ..The absence of detectable changes in ghrelin cells, as seen in immunohistochemistry, could be due to a large intracellular storage of potentially releasable acylghrelin...
  26. doi request reprint Effect of cushioned or single layer semen centrifugation before sex sorting on frozen stallion semen quality
    G Mari
    Department of Veterinary Medical Sciences, University of Bologna, Bologna, Italy Artificial Insemination Center AUB INFA, University of Bologna, Bologna, Italy
    Theriogenology 83:953-8. 2015
    ..Our results suggest that CC and SLC are not able to select those spermatozoa that possess a better ability to withstand sperm processing associated with sperm sorting and freezing. ..
  27. doi request reprint Enhancing insemination performance in pigs through controlled release of encapsulated spermatozoa
    M Faustini
    Department of Veterinary Science and Public Health, University of Milan, Milan, Italy
    Reprod Domest Anim 47:353-8. 2012
    ....
  28. ncbi request reprint DNase I activity in pig MII oocytes: implications in transgenesis
    Augusta Zannoni
    Department of Veterinary Morphophysiology and Animal Production, University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano dell Emilia, Bologna, Italy
    Reproduction 131:461-8. 2006
    ..These results, together with the high efficiency of sperm based transgenesis methods, suggest that the association with spermatozoa protects exogenous DNA from nuclease activities...
  29. ncbi request reprint Food deprivation stimulates the luteolytic capacity in the gilt
    Giovanna Galeati
    Dipartimento di Morfofisiologia Veterinaria e Produzioni Animali DIMORFIPA, Universita di Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia BO, Italy
    Domest Anim Endocrinol 33:281-93. 2007
    ..Results from this study indicate that fasting in prepubertal gilts induced to ovulate stimulates luteal P4 and PGFM production as well as FPr mRNA expression, thus increasing luteolytic susceptibility...
  30. ncbi request reprint Fasting influences steroidogenesis, vascular endothelial growth factor (VEGF) levels and mRNAs expression for VEGF, VEGF receptor type 2 (VEGFR-2), endothelin-1 (ET-1), endothelin receptor type A (ET-A) and endothelin converting enzyme-1 (ECE-1) in newly
    Giovanna Galeati
    Dipartimento di Morfofisiologia Veterinaria e Produzioni Animali, University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia, BO, Italy
    Domest Anim Endocrinol 28:272-84. 2005
    ..The overall conclusion is that all the parameters studied are affected by feed restriction, but the mechanisms activated at luteal level are possibly not fully adequate to compensate for nutrient shortage...
  31. ncbi request reprint Leptin receptor in boar spermatozoa
    Marco De Ambrogi
    Dipartimento di Morfofisiologia Veterinaria e Produzioni Animali DIMORFIPA, Universita di Bologna, Bologna, Italy
    Int J Androl 30:458-61. 2007
    ..These localizations were maintained even in semen washed twice to eliminate seminal plasma. We conclude that Ob-R is present in boar spermatozoa where seminal plasma leptin can exert its effects...