Genomes and Genes
Affiliation: University of Rome Tor Vergata
- Peripheral development of B cells in mouse and manRita Carsetti
Research Center Ospedale Bambino Gesù, Roma, Italy
Immunol Rev 197:179-91. 2004..B-1a and IgM memory B cells may function as a link between the innate and adaptive immune response and thus perform a primordial B-cell function...
- Impairment of the antipolysaccharide response in splenectomized patients is due to the lack of immunoglobulin M memory B cellsRita Carsetti
J Infect Dis 193:1189-90. 2006
- TLR ligation triggers somatic hypermutation in transitional B cells inducing the generation of IgM memory B cellsAlaitz Aranburu
Research Center, Bambino Gesu Children s Hospital, 00165 Rome, Italy
J Immunol 185:7293-301. 2010....
- B cell modulation strategies in autoimmunity: the SLE exampleM Manuela Rosado
Research Center, Ospedale Pediatrico Bambino Gesù IRCCS, Piazza S Onofrio 4, 00165 Roma, Italy
Curr Pharm Des 17:3155-65. 2011..In contrast to conventional immune-suppression, the availability of drugs interfering with B-cell specific pathogenetic pathways gives the possibility to choose therapies tailored to each disease and, possibly, to each patient...
- Human immunoglobulin M memory B cells controlling Streptococcus pneumoniae infections are generated in the spleenStephanie Kruetzmann
Max Plank Institute for Immunology, Department of Developmental Immunology, D 79108 Freiburg, Germany
J Exp Med 197:939-45. 2003..IgM memory B cells, therefore, require the spleen for their generation and/or survival and are responsible for the protection against encapsulated bacteria...
- The loss of IgM memory B cells correlates with clinical disease in common variable immunodeficiencyRita Carsetti
Research Center, Ospedale Pediatrico Bambino Gesu, Rome, Italy
J Allergy Clin Immunol 115:412-7. 2005..Despite the profound hypogammaglobulinemia, some patients do not experience bacterial pneumonia. We have shown that IgM memory B cells and natural antibodies play an important role in the defense against encapsulated bacteria...
- Hepatitis B specific T cell immunity induced by primary vaccination persists independently of the protective serum antibody levelMaria Carollo
Anti infectious Immunity Unit, Department of Infectious, Parasitic and Immune Mediated Diseases, Istituto Superiore di Sanita, Viale Regina Elena 299, Rome, Italy
Vaccine 31:506-13. 2013..These findings suggest that cellular immune response should be considered alongside serological markers as a surrogate of protection...
- Parents as source of pertussis transmission in hospitalized young infantsGiorgio Fedele
Department of Infectious, Parasitic, and Immune Mediated Diseases, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161, Rome, Italy
Infection . 2016..Understanding the dynamics of transmission of pertussis in families is essential to plan effective prevention strategies that could be integrated in pertussis control...
- Inhibition of B-cell proliferation and antibody production by mesenchymal stromal cells is mediated by T cellsMaria Manuela Rosado
1 Immunology Research Area, Ospedale Pediatrico Bambino Gesu, IRCSS, Roma, Italy
Stem Cells Dev 24:93-103. 2015..This information can be relevant for implementing MSC-based therapeutic immune modulation in patients in whom T-cell function is impaired. ..
- B-cell activation with CD40L or CpG measures the function of B-cell subsets and identifies specific defects in immunodeficient patientsEmiliano Marasco
Division of Rheumatology, Ospedale Pediatrico Bambino Gesù IRCCS, Roma, Italy
Eur J Immunol . 2016..Unexpectedly, B cells from SIgAD patients show a reduced proliferative response to CD40L. Our results demonstrate that functional tests are an important tool to assess the functions of the humoral immune system...
- Evaluating B-cells: from bone marrow precursors to antibody-producing cellsM Manuela Rosado
Research Center Ospedale Pediatrico Bambino Gesù IRCCS, Rome, Italy
Methods Mol Biol 1032:45-57. 2013..The number and specificity of ASC can be measured by Enzyme-linked immunosorbent spot (ELISPOT) assay, a variation of the enzyme-linked immunosorbent assay (ELISA) used to quantify serum immunoglobulins. ..
- Preserved antibody levels and loss of memory B cells against pneumococcus and tetanus after splenectomy: tailoring better vaccination strategiesM Manuela Rosado
Immunology Area, Bambino Gesu Children s Hospital IRCCS, Rome, Italy
Eur J Immunol 43:2659-70. 2013....
- Does Breastfeeding Protect Young Infants from Pertussis? Case-Control Study and Immunologic EvaluationElisabetta Pandolfi
1Multifactorial Disease and Complex Phenotype Research Area, Bambino Gesu Children s Hospital, Rome, Italy 2Department of Pediatrics, Bambino Gesù Children Hospital IRCSS, Rome, Italy 3Department of Pediatrics, Sapienza University of Rome, Rome, Italy 4Immunology Unit, Immunology and Pharmacal Therapy Area, Bambino Gesù Children Hospital IRCSS, Rome, Italy 5Department of Infectious, Parasitic and Immuno mediated Disease, Istituto Superiore di Sanita, Rome, Italy 6Virology Unit, Laboratory Department, Bambino Gesu Children s Hospital IRCCS, Rome, Italy 7Department of Radiology, Bambino Gesù Children Hospital IRCSS, Rome, Italy
Pediatr Infect Dis J . 2016..Pertussis infection can be severe in unvaccinated infants. A case control study was conducted to investigate the potential role of breastfeeding in protecting young, unvaccinated infants from pertussis...
- Increased serum IgM, immunodeficiency, and autoimmunity: A clinical seriesAndrea Picchianti Diamanti
Sapienza University of Rome, II School of Medicine, S Andrea University Hospital, Chair and Division of Allergy, Clinical Immunology and Rheumatology, Rome, Italy
Int J Immunopathol Pharmacol 28:547-56. 2015..In particular, autoimmunity may be the first manifestation of the disease in patients with low serum immunoglobulins (Ig) levels. Here we describe a group of patients that share features of immunodeficiency and autoimmunity...
- Induction of regulatory T cells after prophylactic treatment with photopheresis in renal transplant recipientsAndrea Lamioni
Research Center, Bambino Gesu Children s Research Hospital, Rome, Italy
Transplantation 83:1393-6. 2007..These preliminary results suggest that the addition of ECP to standard immunosuppressive therapy induces a tolerogenic shift in the immune system of kidney transplanted patients and may pave the way to preventing chronic rejection...
- Generation of switched memory B cells in response to vaccination in Down syndrome children and their siblingsDiletta Valentini
Pediatric and Infectious Disease Unit, Bambino Gesu Children s Hospital, IRCCS, Rome, Italy
Vaccine 33:6689-96. 2015..Special seasonal influenza and pneumococcal vaccination strategies have been designed for individuals with risk conditions of all ages. Down syndrome individuals are not included in the high-risk categories...
- Purification and immunophenotypic characterization of murine MZ and T2-MZP cellsM Manuela Rosado
Immunology Unit, Research Center Ospedale Pediatrico Bambino Gesù, IRCSS, Piazza S Onofrio 4, 00165, Rome, Italy
Methods Mol Biol 1190:3-16. 2014..We will combine the use of FACS analysis and confocal microscopy to visualize marginal zone B cells in cell suspension and tissue sections, respectively. ..
- Switched memory B cells maintain specific memory independently of serum antibodies: the hepatitis B exampleM Manuela Rosado
Research Center Ospedale Pediatrico Bambino Gesù, IRCSS, Laboratory of Flow Cytometry and B Cell Development, Rome, Italy
Eur J Immunol 41:1800-8. 2011..01) and serum Abs (geometric mean concentration, GMC from 2.9 to 284 mIU/mL), demonstrating that circulating memory B cells effectively respond to Ag challenge even when specific Abs fall under the protective threshold...
- CXCL13, CCL21, and CXCL12 expression in salivary glands of patients with Sjogren's syndrome and MALT lymphoma: association with reactive and malignant areas of lymphoid organizationFrancesca Barone
Divisione di Reumatologia Dipartimento di Clinica e Terapia Applicata, Universita La Sapienza Roma, Rome, Italy
J Immunol 180:5130-40. 2008....
- A novel form of non-X-linked hyperigm associated with growth and pubertal disturbances and with lymphoma developmentViviana Moschese
Department of Pediatrics, University of Rome Tor Vergata, Italy
J Pediatr 148:404-6. 2006..We report on two female patients affected by a novel syndrome associating HIGM, growth and pubertal disturbances, and severe lymphoid hyperplasia with eventual development into lymphomas, suggesting a DNA repair defect...
- Effects of exposure to gradient magnetic fields emitted by nuclear magnetic resonance devices on clonogenic potential and proliferation of human hematopoietic stem cellsMaria Grazia Iachininoto
Department of Transfusion Medicine, Institute of Hematology, Universita Cattolica, Rome, Italy
Bioelectromagnetics 37:201-11. 2016..Accordingly, GMFs did not affect the clonogenic potential of umbilical cord blood CD34+ cells exposed in vitro together with the whole mononuclear cell fraction...
- Repeated vaccinations do not improve specific immune defenses against Hepatitis B in non-responder health care workersSalvatore Zaffina
Health Technology Assessment and Safety Research Unit and Occupational Medicine Service, Department of Laboratories, Ospedale Pediatrico Bambino Gesu, IRCSS, Piazza S Onofrio 4, 00165 Roma, Italy
Vaccine 32:6902-10. 2014....
- Characterization of B-cell maturation in the peripheral immune systemRita Carsetti
Research Center, Ospedale Bambino Gesu, Rome, Italy
Methods Mol Biol 271:25-35. 2004..We describe the method for the staining of the cells and the analysis of the collected data and show examples of the results obtained in normal and mutant mice...
- B cells in SLE: different biological drugs for different pathogenic mechanismsAndrea Picchianti Diamanti
Dipartimento di Clinica e Terapia Medica, Universita La Sapienza di Roma, Policlinico Umberto I, Roma, Italy
Autoimmun Rev 7:143-8. 2007..In contrast to general immuno-suppression, the availability of drugs interfering with specific pathogenetic pathways gives the possibility to choose therapies tailored to each disease in each patient...
- Reversion of resistance to immunosuppressive agents in three patients with psoriatic arthritis by cyclosporine A: modulation of P-glycoprotein functionAndrea Picchianti Diamanti
Department of Medical Sciences, Sapienza University of Rome, 2nd School of Medicine, S Andrea University Hospital, Rome, Italy
Clin Immunol 138:9-13. 2011..This is the first time that CSA has been demonstrated as being able to revert MTX/SSA resistance in PsA...
- High nitric oxide production, secondary to inducible nitric oxide synthase expression, is essential for regulation of the tumour-initiating properties of colon cancer stem cellsMaria Ausiliatrice Puglisi
Department of Internal Medicine and Gastroenterology, Gemelli Hospital, Rome, Italy
J Pathol 236:479-90. 2015..This discovery could shed light on the mechanisms by which NO induces the growth and invasiveness of CC, providing new insights into the link between inflammation and colon tumourigenesis...
- Reduced numbers of switched memory B cells with high terminal differentiation potential in Down syndromeRita Carsetti
Immunology Unit, Immunology and Pharmacotherapy Area, Bambino Gesu Children s Hospital, IRCCS, Rome, Italy Diagnostic Immunology Unit, Department of Laboratories, Bambino Gesu Children s Hospital, IRCCS, Rome, Italy
Eur J Immunol 45:903-14. 2015..Tailored vaccination schedules increasing the number of switched memory B cells may improve protection and reduce the risk of death from infection in DS. ..
- Microvescicles derived from mesenchymal stromal cells are not as effective as their cellular counterpart in the ability to modulate immune responses in vitroAntonella Conforti
1 Department of Pediatric Hematology Oncology, IRCCS Bambino Gesù Children s Hospital, Rome, Italy
Stem Cells Dev 23:2591-9. 2014..The relative clinical benefit of either MSCs or MVs needs to be compared in proper prospective studies...
- B-1a B cells that link the innate and adaptive immune responses are lacking in the absence of the spleenHedda Wardemann
Department of Developmental Immunology, Max Planck Institute for Immunobiology, Freiburg 79108, Germany
J Exp Med 195:771-80. 2002..These findings reveal a hitherto unknown role of the spleen in generating and maintaining the B-1a B cell pool...
- Identification of Endothelin-1 and NR4A2 as CD133-regulated genes in colon cancer cellsMaria Ausiliatrice Puglisi
Institute of Internal Medicine, Universita Cattolica del Sacro Cuore, Rome, Italy
J Pathol 225:305-14. 2011..In conclusion, we have identified a molecular signature associated with potential CSCs and showed for the first time the existence of a functional relationship between CD133, END-1 and NR4A2 expression in colon cancer cells...
- Mechanistic associations of a mild phenotype of immunodysregulation, polyendocrinopathy, enteropathy, x-linked syndromeFabrizio De Benedetti
Direzione Scientifica, IRCCS Ospedale Pediatrico Bambino Gesù, Roma, Italy
Clin Gastroenterol Hepatol 4:653-9. 2006..We describe 2 unrelated patients with IPEX syndrome with a mild clinical phenotype and with novel FOXP3 mutations and the phenotypic and functional characterization of their Treg cells...
- Longitudinal Evaluation of Immune Reconstitution and B-cell Function After Hematopoietic Cell Transplantation for Primary ImmunodeficiencyAlessia Scarselli
University Department of Pediatrics, DPUO, Unit of Immune and Infectious Diseases, Bambino Gesu Children s Hospital, Rome, Italy
J Clin Immunol 35:373-83. 2015....
- Hematopoietic activity in putative mouse primordial germ cell populationsMaria Lucia Scaldaferri
Department of Biomedicine and Prevention, Section of Histology and Embryology, University of Rome Tor Vergata, Rome, Italy
Mech Dev 136:53-63. 2015..These results are discussed within of the current model of germline segregation. ..
- A metaproteomic pipeline to identify newborn mouse gut phylotypesFederica del Chierico
Parasitology Unit, Bambino Gesu Children s Hospital, IRCCS, Rome, Italy Metagenomics Unit, Bambino Gesu Children s Hospital, IRCCS, Rome, Italy
J Proteomics 97:17-26. 2014....
- The immunological effects of extracorporeal photopheresis unraveled: induction of tolerogenic dendritic cells in vitro and regulatory T cells in vivoAndrea Lamioni
Research Center, Ospedale Bambino Gesu, Piazza S Onofrio 4, 00165 Roma, Italy
Transplantation 79:846-50. 2005..The immunomodulatory effects of ECP may be explained by its ability to increase the frequency of regulatory T cells with inhibitory action on transplant immune rejection...
- Folic acid and methionine in the prevention of teratogen-induced congenital defects in miceDaria Cipollone
Department of Biopathology and Diagnostic Imaging, University Tor Vergata, Rome, Italy
Cardiovasc Pathol 18:100-9. 2009..Hereby, we analyzed the effects of nutritive therapy involving folic acid and methionine on teratogen-induced congenital defects in mice...
- Pharmacological inhibition of TLR9 activation blocks autoantibody production in human B cells from SLE patientsFederica Capolunghi
Cytometry and B cell Development Unit, Research Center, Department of Nephrology and Urology, Children Hospital Bambino Gesù, and Department of Public Health and Cell Biology, University of Rome Tor Vergata, Rome, Italy
Rheumatology (Oxford) 49:2281-9. 2010..Here, we investigated whether memory B cells produce autoantibodies in SLE patients with active disease or in clinical remission, and whether ST2825 could inhibit PC generation in SLE patients...
- Impairment of splenic IgM-memory but not switched-memory B cells in a patient with celiac disease and splenic atrophyAntonio Di Sabatino
J Allergy Clin Immunol 120:1461-3. 2007
- Depletion of immunoglobulin M memory B cells is associated with splenic hypofunction in inflammatory bowel diseaseAntonio Di Sabatino
Gastroenterology Unit, IRCCS Policlinico S Matteo, University of Pavia, Pavia, Italy
Am J Gastroenterol 100:1788-95. 2005....
- Splenic hypofunction and the spectrum of autoimmune and malignant complications in celiac diseaseAntonio Di Sabatino
First Department of Medicine, Istituto di Ricovero e Cura a Carattere Scientifico Policlinico S Matteo, University of Pavia, Piazzale Golgi 5, 27100 Pavia, Italy
Clin Gastroenterol Hepatol 4:179-86. 2006....
- CpG drives human transitional B cells to terminal differentiation and production of natural antibodiesFederica Capolunghi
Laboratory of B cell Development Research Center, Department of Medical and Surgical Neonatology, Children Hospital Bambino Gesù, and Department of Clinical Medicine, University of Rome La Sapienza, Italy
J Immunol 180:800-8. 2008..Therefore, the ability of transitional B cells to sense bacterial DNA through TLR9 represents a tool to rapidly build up the repertoire of natural Abs necessary for our first-line defense at birth...
- Splenic function and IgM-memory B cells in Crohn's disease patients treated with infliximabAntonio Di Sabatino
First Department of Medicine, Fondazione IRCCS Policlinico S Matteo, University of Pavia, Pavia, Italy
Inflamm Bowel Dis 14:591-6. 2008..On this basis, we aimed to investigate the influence of anti-TNF therapy on splenic function in CD patients...
- Activation of T cells via tumor antigen specific chimeric receptors: the role of the intracellular signaling domainFlorian O Losch
Department of Molecular Immunology, Biology III, University of Freiburg and Max Planck Institute for Immunobiology, Germany
Int J Cancer 103:399-407. 2003..Given their specificity and efficiency, T cells grafted with these type of receptors may represent potential candidates for cancer passive immunotherapy...
- Memory B-cell subsets as a predictive marker of outcome in hypogammaglobulinemia during infancyViviana Moschese
J Allergy Clin Immunol 120:474-6. 2007
- A novel immunodeficiency characterized by the exclusive presence of transitional B cells unresponsive to CpGAlessandro Plebani
Clinica Pediatrica and Istituto di Medicina Molecolare A Nocivelli, Universita di Brescia, Italy
Immunology 121:183-8. 2007..This defines a novel form of primary immunodeficiency mimicking common variable immunodeficiency in the presence of normal immunoglobulin serum levels...
- Development and function of the mammalian spleenAndrea Brendolan
Department of Cell and Developmental Biology, Cornell University, Weill Medical School, New York, NY, USA
Bioessays 29:166-77. 2007..The lack of these populations in asplenic mice and humans may go some way to explaining this susceptibility...