Andrea Battistoni

Summary

Affiliation: University of Rome Tor Vergata
Country: Italy

Publications

  1. ncbi request reprint Correction: Zinc is required to ensure the expression of flagella and the ability to form biofilms in Salmonella enterica sv Typhimurium
    Serena Ammendola
    Dipartimento di Biologia, Universita di Roma Tor Vergata, Via della Ricerca Scientifica, 00133 Rome, Italy
    Metallomics 8:1217. 2016
  2. ncbi request reprint Zinc is required to ensure the expression of flagella and the ability to form biofilms in Salmonella enterica sv Typhimurium
    Serena Ammendola
    Dipartimento di Biologia, Universita di Roma Tor Vergata, Via della Ricerca Scientifica, 00133 Rome, Italy
    Metallomics 8:1131-1140. 2016
  3. pmc The capability of Pseudomonas aeruginosa to recruit zinc under conditions of limited metal availability is affected by inactivation of the ZnuABC transporter
    Melania D'Orazio
    Department of Biology, University of Rome Tor Vergata, 00133 Roma, Italy
    Metallomics 7:1023-35. 2015
  4. pmc Low-Shear Modeled Microgravity Enhances Salmonella Enterica Resistance to Hydrogen Peroxide Through a Mechanism Involving KatG and KatN
    Francesca Pacello
    Dipartimento di Biologia, Universita di Roma Tor Vergata, 00133 Rome
    Open Microbiol J 6:53-64. 2012
  5. pmc Extracellular glutathione decreases the ability of Burkholderia cenocepacia to penetrate into epithelial cells and to induce an inflammatory response
    Melania D'Orazio
    Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele Pisana, Roma, Italy
    PLoS ONE 7:e47550. 2012
  6. pmc The ZupT transporter plays an important role in zinc homeostasis and contributes to Salmonella enterica virulence
    Mauro Cerasi
    Dipartimento di Biologia, Universita di Roma Tor Vergata, Via della Ricerca Scientifica, 00133 Rome, Italy
    Metallomics 6:845-53. 2014
  7. pmc Diversity of Salmonella spp. serovars isolated from the intestines of water buffalo calves with gastroenteritis
    Giorgia Borriello
    Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute, 2, 80055, Portici, Italy
    BMC Vet Res 8:201. 2012
  8. pmc Role of ZnuABC and ZinT in Escherichia coli O157:H7 zinc acquisition and interaction with epithelial cells
    Roberta Gabbianelli
    Biotechnology Service of Service for Biotechnology and Animal Welfare, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161 Rome, Italy
    BMC Microbiol 11:36. 2011
  9. pmc Increased expression of periplasmic Cu,Zn superoxide dismutase enhances survival of Escherichia coli invasive strains within nonphagocytic cells
    A Battistoni
    Department of Biology, Universita di Roma Tor Vergata, 00133 Rome, Italy
    Infect Immun 68:30-7. 2000
  10. ncbi request reprint Involvement of reactive oxygen species in bacterial killing within epithelial cells
    A Battistoni
    Dipartmento di Biologia, Università di Rome Tor Vergata, Rome, Italy
    Int J Immunopathol Pharmacol 17:71-6. 2004

Collaborators

Detail Information

Publications45

  1. ncbi request reprint Correction: Zinc is required to ensure the expression of flagella and the ability to form biofilms in Salmonella enterica sv Typhimurium
    Serena Ammendola
    Dipartimento di Biologia, Universita di Roma Tor Vergata, Via della Ricerca Scientifica, 00133 Rome, Italy
    Metallomics 8:1217. 2016
    ..Correction for 'Zinc is required to ensure the expression of flagella and the ability to form biofilms in Salmonella enterica sv Typhimurium' by Serena Ammendola et al., Metallomics, 2016, DOI: 10.1039/c6mt00108d...
  2. ncbi request reprint Zinc is required to ensure the expression of flagella and the ability to form biofilms in Salmonella enterica sv Typhimurium
    Serena Ammendola
    Dipartimento di Biologia, Universita di Roma Tor Vergata, Via della Ricerca Scientifica, 00133 Rome, Italy
    Metallomics 8:1131-1140. 2016
    ..On the whole, our study reveals novel roles of zinc in Salmonella motility and intercellular communication...
  3. pmc The capability of Pseudomonas aeruginosa to recruit zinc under conditions of limited metal availability is affected by inactivation of the ZnuABC transporter
    Melania D'Orazio
    Department of Biology, University of Rome Tor Vergata, 00133 Roma, Italy
    Metallomics 7:1023-35. 2015
    ..These results indicate that efficient zinc acquisition is critical for the expression of various virulence features typical of P. aeruginosa and that ZnuABC also plays an important role in zinc homeostasis in this microorganism. ..
  4. pmc Low-Shear Modeled Microgravity Enhances Salmonella Enterica Resistance to Hydrogen Peroxide Through a Mechanism Involving KatG and KatN
    Francesca Pacello
    Dipartimento di Biologia, Universita di Roma Tor Vergata, 00133 Rome
    Open Microbiol J 6:53-64. 2012
    ....
  5. pmc Extracellular glutathione decreases the ability of Burkholderia cenocepacia to penetrate into epithelial cells and to induce an inflammatory response
    Melania D'Orazio
    Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele Pisana, Roma, Italy
    PLoS ONE 7:e47550. 2012
    ..It is not known whether this defect may favor lung colonization by opportunistic pathogens...
  6. pmc The ZupT transporter plays an important role in zinc homeostasis and contributes to Salmonella enterica virulence
    Mauro Cerasi
    Dipartimento di Biologia, Universita di Roma Tor Vergata, Via della Ricerca Scientifica, 00133 Rome, Italy
    Metallomics 6:845-53. 2014
    ..Altogether, the here reported results show that ZupT plays an important role in Salmonella zinc homeostasis, being involved in metal import both in vitro and in infected animals. ..
  7. pmc Diversity of Salmonella spp. serovars isolated from the intestines of water buffalo calves with gastroenteritis
    Giorgia Borriello
    Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute, 2, 80055, Portici, Italy
    BMC Vet Res 8:201. 2012
    ..In the present study, the prevalence of Salmonella spp. in water buffalo calves affected by lethal gastroenteritis was assessed, and a polyphasic characterization of isolated strains of S. Typhimurium was performed...
  8. pmc Role of ZnuABC and ZinT in Escherichia coli O157:H7 zinc acquisition and interaction with epithelial cells
    Roberta Gabbianelli
    Biotechnology Service of Service for Biotechnology and Animal Welfare, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161 Rome, Italy
    BMC Microbiol 11:36. 2011
    ..To better understand the role of zinc in bacterial pathogenicity, we have undertaken a functional characterization of the role of the ZnuABC-mediated zinc uptake pathway in enterohemorrhagic Escherichia coli O157:H7...
  9. pmc Increased expression of periplasmic Cu,Zn superoxide dismutase enhances survival of Escherichia coli invasive strains within nonphagocytic cells
    A Battistoni
    Department of Biology, Universita di Roma Tor Vergata, 00133 Rome, Italy
    Infect Immun 68:30-7. 2000
    ..We propose that Cu,Zn superoxide dismutase could offer an important selective advantage in survival within host cells to bacteria expressing high levels of this enzyme...
  10. ncbi request reprint Involvement of reactive oxygen species in bacterial killing within epithelial cells
    A Battistoni
    Dipartmento di Biologia, Università di Rome Tor Vergata, Rome, Italy
    Int J Immunopathol Pharmacol 17:71-6. 2004
    ....
  11. ncbi request reprint Role of prokaryotic Cu,Zn superoxide dismutase in pathogenesis
    A Battistoni
    Department of Biology, University of Rome Tor Vergata, Via della Ricerca Scientifica, 00133 Rome, Italy
    Biochem Soc Trans 31:1326-9. 2003
    ..In fact, several enzyme variants exhibit unique properties that may lead to the acquisition of novel specialized functions distinct from superoxide dismutation...
  12. ncbi request reprint A histidine-rich metal binding domain at the N terminus of Cu,Zn-superoxide dismutases from pathogenic bacteria: a novel strategy for metal chaperoning
    A Battistoni
    Dipartimento di Biologia, Universita di Roma Tor Vergata, Via della Ricerca Scientifica, 00133 Rome, Italy
    J Biol Chem 276:30315-25. 2001
    ..Particular biological importance for this domain is inferred by the observation that its presence enhances the protection offered by periplasmic Cu,Zn-superoxide dismutase toward phagocytic killing...
  13. doi request reprint Carbon monoxide binding to the heme group at the dimeric interface modulates structure and copper accessibility in the Cu,Zn superoxide dismutase from Haemophilus ducreyi: in silico and in vitro evidences
    Giovanni Chillemi
    CASPUR, Consortium for Supercomputing Applications, Rome, Italy
    J Biomol Struct Dyn 30:269-79. 2012
    ..Altogether, our results support the hypothesis that the HdSOD is a heme-sensor protein, in which binding to small gaseous molecules modulates the enzyme superoxide activity as an adaptive response to the bacterial environment...
  14. pmc Regulatory and structural differences in the Cu,Zn-superoxide dismutases of Salmonella enterica and their significance for virulence
    Serena Ammendola
    Dipartimento di Biologia, Universita di Roma Tor Vergata, 00133 Rome, Italy
    J Biol Chem 283:13688-99. 2008
    ..Our observations demonstrate that differences in expression, activity, and stability help to account for the unique contribution of the bacteriophage-encoded SodCI enzyme to Salmonella virulence...
  15. pmc High-affinity Zn2+ uptake system ZnuABC is required for bacterial zinc homeostasis in intracellular environments and contributes to the virulence of Salmonella enterica
    Serena Ammendola
    Department of Biology, University of Rome Tor Vergata, Via della Ricerca Scientifica, 00133 Roma, Italy
    Infect Immun 75:5867-76. 2007
    ..These results shed new light on the complex functions of zinc in vertebrate and bacterial physiology and pave the way for a better comprehension of pathogenic mechanisms in Salmonella infections...
  16. ncbi request reprint A novel heme protein, the Cu,Zn-superoxide dismutase from Haemophilus ducreyi
    F Pacello
    Dipartimento di Biologia and INFM, , Via della Ricerca Scientifica, 00133 Roma, Italy
    J Biol Chem 276:30326-34. 2001
    ..Our results suggest that periplasmic Cu,Zn-superoxide dismutase plays a role in heme metabolism of H. ducreyi and provide further evidence for the structural flexibility of bacterial enzymes of this class...
  17. doi request reprint Structural-dynamical investigation of the ZnuA histidine-rich loop: involvement in zinc management and transport
    Mattia Falconi
    Department of Biology, University of Rome Tor Vergata, Via della Ricerca Scientifica, 00133 Rome, Italy
    J Comput Aided Mol Des 25:181-94. 2011
    ..Based on the plasticity of the histidine-rich loop and its significant effects on protein mobility a possible role in the capture and/or transfer of the zinc ions has been suggested...
  18. pmc The Zur-regulated ZinT protein is an auxiliary component of the high-affinity ZnuABC zinc transporter that facilitates metal recruitment during severe zinc shortage
    Patrizia Petrarca
    Dipartimento di Biologia Università di Roma Tor Vergata, 00133 Rome, Italy
    J Bacteriol 192:1553-64. 2010
    ..Although the presence of ZinT is not strictly required to ensure the functionality of the ZnuABC transporter, our data suggest that ZinT facilitates metal acquisition during severe zinc shortage...
  19. doi request reprint Thermal stability and redox properties of M. tuberculosis CuSOD
    Melania D'Orazio
    Department of Biology, University of Rome Tor Vergata, Italy
    Arch Biochem Biophys 486:119-24. 2009
    ..tuberculosis Cu,SOD compensate for the absence of zinc in achieving a fully active enzyme...
  20. pmc Regulatory and structural properties differentiating the chromosomal and the bacteriophage-associated Escherichia coli O157:H7 Cu, Zn superoxide dismutases
    Melania D'Orazio
    Department of Biology, University of Rome, Tor Vergata, Rome, Italy
    BMC Microbiol 8:166. 2008
    ..coli strains, and sodC-F1 and sodC-F2, two nearly identical genes located within lambdoid prophage sequences. The significance of this apparent sodC redundancy in E. coli O157:H7 has not yet been investigated...
  21. doi request reprint Periplasmic Cu,Zn superoxide dismutase and cytoplasmic Dps concur in protecting Salmonella enterica serovar Typhimurium from extracellular reactive oxygen species
    Francesca Pacello
    Dipartimento di Biologia, Universita di Roma Tor Vergata, Via della Ricerca Scientifica, 00133 Roma, Italy
    Biochim Biophys Acta 1780:226-32. 2008
    ..Moreover, the nearly additive effect of the sodC and dps mutations suggests that localization of antioxidant enzymes in different cellular compartments is required for bacterial resistance to extracytoplasmic oxidative attack...
  22. ncbi request reprint Differential contribution of sodC1 and sodC2 to intracellular survival and pathogenicity of Salmonella enterica serovar Choleraesuis
    Serena Ammendola
    Dipartimento di Biologia, Universita di Roma Tor Vergata, Via della Ricerca Scientifica, 00133 Rome, Italy
    Microbes Infect 7:698-707. 2005
    ..enterica serovar Typhimurium and suggest that the relative contributions of sodC1 and sodC2 to host-pathogen interactive biology may vary depending on the Salmonella serovar or strain...
  23. doi request reprint Zinc ion coordination as a modulating factor of the ZnuA histidine-rich loop flexibility: a molecular modeling and fluorescence spectroscopy study
    Silvia Castelli
    Department of Biology, University of Rome Tor Vergata, Via della Ricerca Scientifica, 00133 Rome, Italy
    Biochem Biophys Res Commun 430:769-73. 2013
    ..The data obtained by fluorescence spectroscopy have then be used to validate a 3D model including the unsolved histidine-rich loop...
  24. doi request reprint Fe-heme structure in Cu, Zn superoxide dismutase from Haemophilus ducreyi by X-ray absorption spectroscopy
    Paola D'Angelo
    Department of Chemistry University La Sapienza, Rome, Italy
    Arch Biochem Biophys 498:43-9. 2010
    ....
  25. ncbi request reprint Single mutations at the subunit interface modulate copper reactivity in Photobacterium leiognathi Cu,Zn superoxide dismutase
    M E Stroppolo
    Department of Biology and INFM, University of Rome Tor Vergata, Rome, Via della Ricerca Scientifica, 00133, Italy
    J Mol Biol 308:555-63. 2001
    ..Such long-range intramolecular communication effects have not been extensively described before within the Cu,Zn superoxide dismutase homology family...
  26. pmc The Cu,Zn superoxide dismutase from Escherichia coli retains monomeric structure at high protein concentration. Evidence for altered subunit interaction in all the bacteriocupreins
    A Battistoni
    Department of Biology, University of Rome Tor Vergata, Italy
    Biochem J 320:713-6. 1996
    ..The substitution of hydrophobic residues with charged ones at positions located at the dimer interface of all known Cu,Zn superoxide dismutases could be specifically responsible for the monomeric structure of the E. coli enzyme...
  27. ncbi request reprint Periplasmic competition for zinc uptake between the metallochaperone ZnuA and Cu,Zn superoxide dismutase
    Giovanni Berducci
    Dipartimento di Biologia, Universita di Roma Tor Vergata, Via della Ricerca Scientifica, 00133 Rome, Italy
    FEBS Lett 569:289-92. 2004
    ..The effective ZnuA ability to sequester the available zinc ions from the periplasm suggests that zinc-containing cytoplasmic proteins are more important for bacterial viability than the periplasmic ones...
  28. pmc Competition for zinc binding in the host-pathogen interaction
    Mauro Cerasi
    Dipartimento di Biologia, Università di Roma Tor Vergata Rome, Italy
    Front Cell Infect Microbiol 3:108. 2013
    ....
  29. doi request reprint CD4+CD25+ T regulatory cells limit effector T cells and favor the progression of brucellosis in BALB/c mice
    Paolo Pasquali
    Department of Food Safety and Veterinary Public Health, Istituto Superiore di Sanita, Rome, Italy
    Microbes Infect 12:3-10. 2010
    ..We demonstrate that CD4+CD25+T regulatory cells are able to limit the effectiveness of CD4+T cells and are able to favor the maintenance and the progression of B. abortus infection...
  30. ncbi request reprint Mutations of Gly to Ala in human glutathione transferase P1-1 affect helix 2 (G-site) and induce positive cooperativity in the binding of glutathione
    M Lo Bello
    Department of Biology, University of Rome Tor Vergata, Italy
    J Mol Biol 284:1717-25. 1998
    ..Molecular modeling of the Gly41Ala/Gly50Ala mutant and of the inactive oxidized form of the native enzyme provides a structural explanation of our results...
  31. pmc Lipid modification of the Cu,Zn superoxide dismutase from Mycobacterium tuberculosis
    M D'Orazio
    Department of Biology, University of Rome Tor Vergata, Via della Ricerca Scientifica, 00133 Rome, Italy
    Biochem J 359:17-22. 2001
    ..Evidence is also provided for an up-regulation of M. tuberculosis sodC in response to phagocytosis by human macrophages, suggesting that Cu,ZnSOD is involved in the mechanisms that facilitate mycobacterial intracellular growth...
  32. ncbi request reprint Unfolding and inactivation of monomeric superoxide dismutase from E. coli by SDS
    M Bozzi
    Department of Chemical Science and Technology, Tor Vergata University, Via della Ricerca Scientifica, 00133, Rome, Italy
    Int J Biol Macromol 29:99-105. 2001
    ..The unfolding is reversible and both, the tridimensional structure of the protein and the active site, can be restored upon dialysis. In addition, unfolding occurs without loss of metals in the solution...
  33. ncbi request reprint Role of the electrostatic loop of Cu,Zn superoxide dismutase in the copper uptake process
    M R Ciriolo
    Department of Biomedical Sciences, University of Chieti G D Annunzio, Via dei Vestini 66100 Chieti, Italy
    Eur J Biochem 268:737-42. 2001
    ....
  34. pmc Reactive oxygen species and epidermal growth factor are antagonistic cues controlling SHP-2 dimerization
    Aurelio Pio Nardozza
    Department of Biology, University of Rome Tor Vergata, Rome, Italy
    Mol Cell Biol 32:1998-2009. 2012
    ..Although this differential behavior cannot be rationalized mechanistically yet, these findings suggest a possible regulatory role of dimerization in SHP-2 function...
  35. doi request reprint 10-undecanhydroxamic acid, a hydroxamate derivative of the undecanoic acid, has strong antimicrobial activity through a mechanism that limits iron availability
    Serena Ammendola
    Dipartimento di Biologia, Universita di Roma Tor Vergata, Rome, Italy
    FEMS Microbiol Lett 294:61-7. 2009
    ....
  36. pmc An ERp57-mediated disulphide exchange promotes the interaction between Burkholderia cenocepacia and epithelial respiratory cells
    Francesca Pacello
    Department of Biology, University of Rome Tor Vergata, Roma, Italy
    Sci Rep 6:21140. 2016
    ....
  37. doi request reprint Deregulation of transition metals homeostasis is a key feature of cadmium toxicity in Salmonella
    Serena Ammendola
    Department of Biology, University of Rome Tor Vergata, Via della Ricerca Scientifica, 00133, Rome, Italy
    Biometals 27:703-14. 2014
    ..Our results indicate that cadmium interferes with the ability of cells to manage transition metals and highlight the close interconnections between the homeostatic mechanisms regulating the intracellular levels of different metals. ..
  38. ncbi request reprint Flexibility in monomeric Cu,Zn superoxide dismutase detected by limited proteolysis and molecular dynamics simulation
    M Falconi
    INFM and Department of Biology, University of Rome Tor Vergata, Rome, Italy
    Proteins 47:513-20. 2002
    ..These experiments demonstrate that molecular dynamics simulation and limited proteolysis are complementary and unambiguous tools to identify flexible sites in proteins...
  39. ncbi request reprint Isolation of an active and heat-stable monomeric form of Cu,Zn superoxide dismutase from the periplasmic space of Escherichia coli
    A Battistoni
    Department of Biology, University of Rome Tor Vergata, Italy
    FEBS Lett 374:199-202. 1995
    ..Furthermore the purified enzyme proved to be highly heat-stable and, uniquely among CuZnSODs, protease-sensitive. The latter property may explain the previously described lability of this protein in cell extracts...
  40. pmc Xenopus laevis Cu,Zn superoxide dismutase B cDNA sequence
    M T Carri
    Dipartimento di Biologia, II Universita di Roma Tor Vergata, Italy
    Nucleic Acids Res 18:1641. 1990
  41. ncbi request reprint Bovine lactoferrin inhibits the efficiency of invasion of respiratory A549 cells of different iron-regulated morphological forms of Pseudomonas aeruginosa and Burkholderia cenocepacia
    F Berlutti
    Department of Public Health Sciences, University Sapienza, Rome, Italy
    Int J Immunopathol Pharmacol 21:51-9. 2008
    ..cenocepacia was strongly inhibited by the presence of bLf, independently of its degree of iron-binding activity. This is the first report demonstrating an anti-invasive property of bLf for strains of P. aeruginosa and B. cenocepacia...
  42. ncbi request reprint Vibrio cholerae periplasmic superoxide dismutase: isolation of the gene and overexpression of the protein
    R Gabbianelli
    Biological Service, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161 Rome, Italy
    J Biotechnol 109:123-30. 2004
    ..This property, which appears to be common to other bacterial enzymes of this class, might improve the ability of Cu,Zn superoxide dismutase to protect bacteria against the reactive oxygen species produced by phagocytes...
  43. doi request reprint The dimeric assembly of Photobacterium leiognathi and Salmonella typhimurium SodC1 Cu,Zn superoxide dismutases is affected differently by active site demetallation and pH: an analytical ultracentrifuge study
    B Catacchio
    CNR Institute of Molecular Biology and Pathology, Department of Biochemical Sciences, Sapienza University of Rome, P le Aldo Moro 5, 00185 Rome, Italy
    Arch Biochem Biophys 471:77-84. 2008
    ..0 favors monomerization. These effects prove the existence of long-range communication between the active site and the subunit interface and provide a structural explanation for the known functional differences between the two enzymes...
  44. ncbi request reprint Unique structural features of the monomeric Cu,Zn superoxide dismutase from Escherichia coli, revealed by X-ray crystallography
    A Pesce
    Dipartimento di Fisica and INFM, Universita di Genova, Largo Rosanna Benzi, 10, 16132 Genova, Italy
    J Mol Biol 274:408-20. 1997
    ..The molecular surface region involved in subunit dimerization in eukaryotic superoxide dismutases is structurally altered in E_SOD and displays a net polar nature...
  45. ncbi request reprint Functional and crystallographic characterization of Salmonella typhimurium Cu,Zn superoxide dismutase coded by the sodCI virulence gene
    A Pesce
    Department of Physics INFM and Advanced Biotechnology Center IST, University of Genoa, Largo Rosanna Benzi, Genova, 10 I 16132, Italy
    J Mol Biol 302:465-78. 2000
    ..The crystallographic and functional characterization of this Salmonella Cu,Zn superoxide dismutase indicates that structural variability and catalytic efficiency are higher in prokaryotic than in the eukaryotic homologous enzymes...