Paola Ascenzi

Summary

Affiliation: University Roma Tre
Country: Italy

Publications

  1. ncbi request reprint Hemoglobin and heme scavenging
    Paolo Ascenzi
    National Institute for Infectious Diseases IRCCS Lazzaro Spallanzani, Roma, Italy
    IUBMB Life 57:749-59. 2005
  2. doi request reprint The nitrite reductase activity of horse heart carboxymethylated-cytochrome c is modulated by cardiolipin
    Paolo Ascenzi
    Interdepartmental Laboratory for Electron Microscopy, Roma Tre University, Via della Vasca Navale 79, 00146, Rome, Italy
    J Biol Inorg Chem 21:421-32. 2016
  3. doi request reprint Cyanide binding to ferrous and ferric microperoxidase-11
    Paolo Ascenzi
    Interdepartmental Laboratory for Electron Microscopy, Roma Tre University, Via della Vasca Navale 79, 00146, Rome, Italy
    J Biol Inorg Chem 21:511-22. 2016
  4. doi request reprint Neuroglobin: From structure to function in health and disease
    Paolo Ascenzi
    Laboratorio Interdipartimentale di Microscopia Eletronica, Universita Roma Tre, Via della Vasca Navale 79, Roma I 00146, Italy Electronic address
    Mol Aspects Med 52:1-48. 2016
  5. doi request reprint NO2(-)-mediated nitrosylation of ferrous microperoxidase-11
    Paolo Ascenzi
    Interdepartmental Laboratory for Electron Microscopy, Roma Tre University, I 00146 Roma, Italy Institute of Protein Biochemistry, National Research Council, I 80131, Napoli, Italy Electronic address
    J Inorg Biochem 153:121-7. 2015
  6. doi request reprint Structural Biology of Bacterial Haemophores
    Paolo Ascenzi
    Laboratorio Interdipartimentale di Microscopia Elettronica, Universita Roma Tre, Roma, Italy Istituto di Bioscienze e BioRisorse, Consiglio Nazionale delle Ricerche, Napoli, Italy Electronic address
    Adv Microb Physiol 67:127-76. 2015
  7. ncbi request reprint Drugs modulate allosterically heme-Fe-recognition by human serum albumin and heme-fe-mediated reactivity
    Alessandra di Masi
    Interdepartmental Laboratory for Electron Microscopy, Roma Tre University, Via della Vasca Navale 79, I 00146 Roma, Italy
    Curr Pharm Des 21:1837-47. 2015
  8. doi request reprint Ferric microperoxidase-11 catalyzes peroxynitrite isomerization
    Paolo Ascenzi
    Interdepartmental Laboratory for Electron Microscopy, Roma Tre University, I 00146 Roma, Italy Institute of Protein Biochemistry National Research Council, I 80131, Napoli, Italy Electronic address
    J Inorg Biochem 144:56-61. 2015
  9. doi request reprint Cardiolipin modulates allosterically the nitrite reductase activity of horse heart cytochrome c
    Paolo Ascenzi
    Interdepartmental Laboratory for Electron Microscopy, University Roma Tre, Via della Vasca Navale 79, 00146, Rome, Italy
    J Biol Inorg Chem 19:1195-201. 2014
  10. pmc Isoniazid inhibits the heme-based reactivity of Mycobacterium tuberculosis truncated hemoglobin N
    Paolo Ascenzi
    Interdepartmental Laboratory of Electron Microscopy, University Roma Tre, Roma, Italy
    PLoS ONE 8:e69762. 2013

Detail Information

Publications114 found, 100 shown here

  1. ncbi request reprint Hemoglobin and heme scavenging
    Paolo Ascenzi
    National Institute for Infectious Diseases IRCCS Lazzaro Spallanzani, Roma, Italy
    IUBMB Life 57:749-59. 2005
    ..Here, hemoglobin and heme trapping mechanisms are summarized...
  2. doi request reprint The nitrite reductase activity of horse heart carboxymethylated-cytochrome c is modulated by cardiolipin
    Paolo Ascenzi
    Interdepartmental Laboratory for Electron Microscopy, Roma Tre University, Via della Vasca Navale 79, 00146, Rome, Italy
    J Biol Inorg Chem 21:421-32. 2016
    ..These results indicate that Met80 carboxymethylation and CL binding cooperate in the stabilization of the highly reactive heme-Fe atom of CL-CM-cytc. ..
  3. doi request reprint Cyanide binding to ferrous and ferric microperoxidase-11
    Paolo Ascenzi
    Interdepartmental Laboratory for Electron Microscopy, Roma Tre University, Via della Vasca Navale 79, 00146, Rome, Italy
    J Biol Inorg Chem 21:511-22. 2016
    ..Penta-coordinated MP11 displays a very high reactivity towards cyanide, whereas the reactivity of hexa-coordinated horse heart cytochrome c is very low. ..
  4. doi request reprint Neuroglobin: From structure to function in health and disease
    Paolo Ascenzi
    Laboratorio Interdipartimentale di Microscopia Eletronica, Universita Roma Tre, Via della Vasca Navale 79, Roma I 00146, Italy Electronic address
    Mol Aspects Med 52:1-48. 2016
    ..Here, structural and functional aspects of human Ngb are examined critically to highlight its roles in health and disease...
  5. doi request reprint NO2(-)-mediated nitrosylation of ferrous microperoxidase-11
    Paolo Ascenzi
    Interdepartmental Laboratory for Electron Microscopy, Roma Tre University, I 00146 Roma, Italy Institute of Protein Biochemistry, National Research Council, I 80131, Napoli, Italy Electronic address
    J Inorg Biochem 153:121-7. 2015
    ..Present results highlight the role of heme distal residues in modulating horse heart cyt c reactivity. ..
  6. doi request reprint Structural Biology of Bacterial Haemophores
    Paolo Ascenzi
    Laboratorio Interdipartimentale di Microscopia Elettronica, Universita Roma Tre, Roma, Italy Istituto di Bioscienze e BioRisorse, Consiglio Nazionale delle Ricerche, Napoli, Italy Electronic address
    Adv Microb Physiol 67:127-76. 2015
    ..Here, structural biology of bacterial haemophores is reviewed focusing on haem acquisition, haem internalization, and haem-degrading systems. ..
  7. ncbi request reprint Drugs modulate allosterically heme-Fe-recognition by human serum albumin and heme-fe-mediated reactivity
    Alessandra di Masi
    Interdepartmental Laboratory for Electron Microscopy, Roma Tre University, Via della Vasca Navale 79, I 00146 Roma, Italy
    Curr Pharm Des 21:1837-47. 2015
    ..Remarkably, heme-Fe-mediated HSA properties are time-dependent, representing a case for "chronosteric effects". Here, we review the drug-based modulation of (i) heme-Fe-recognition by HSA and (ii) heme-Fe-mediated reactivity. ..
  8. doi request reprint Ferric microperoxidase-11 catalyzes peroxynitrite isomerization
    Paolo Ascenzi
    Interdepartmental Laboratory for Electron Microscopy, Roma Tre University, I 00146 Roma, Italy Institute of Protein Biochemistry National Research Council, I 80131, Napoli, Italy Electronic address
    J Inorg Biochem 144:56-61. 2015
    ..Present results highlight the role of the heme-Fe(III) co-ordination state in the modulation of cytochrome c reactivity. ..
  9. doi request reprint Cardiolipin modulates allosterically the nitrite reductase activity of horse heart cytochrome c
    Paolo Ascenzi
    Interdepartmental Laboratory for Electron Microscopy, University Roma Tre, Via della Vasca Navale 79, 00146, Rome, Italy
    J Biol Inorg Chem 19:1195-201. 2014
    ....
  10. pmc Isoniazid inhibits the heme-based reactivity of Mycobacterium tuberculosis truncated hemoglobin N
    Paolo Ascenzi
    Interdepartmental Laboratory of Electron Microscopy, University Roma Tre, Roma, Italy
    PLoS ONE 8:e69762. 2013
    ..These data suggest a direct role of isoniazid to impair fundamental functions of mycobacteria, e.g. scavenging of reactive nitrogen and oxygen species, and metabolism...
  11. doi request reprint Warfarin modulates the nitrite reductase activity of ferrous human serum heme-albumin
    Paolo Ascenzi
    Interdepartmental Laboratory of Electron Microscopy, University Roma Tre, Via della Vasca Navale 79, 00146, Rome, Italy
    J Biol Inorg Chem 18:939-46. 2013
    ..g., warfarin) in modulating HSA(-heme-Fe) functions, and (3) strongly support the view that HSA acts not only as a heme carrier but also displays transient heme-based reactivity. ..
  12. doi request reprint Non-covalent and covalent modifications modulate the reactivity of monomeric mammalian globins
    Paolo Ascenzi
    Interdepartmental Laboratory of Electron Microscopy, University Roma Tre, Roma, Italy
    Biochim Biophys Acta 1834:1750-6. 2013
    ..This article is part of a Special Issue entitled: Oxygen Binding and Sensing Proteins. ..
  13. doi request reprint Reductive nitrosylation of Methanosarcina acetivorans protoglobin: a comparative study
    Paolo Ascenzi
    Laboratorio Interdipartimentale di Microscopia Elettronica, Universita Roma Tre, Via della Vasca Navale 79, I 00146 Roma, Italy
    Biochem Biophys Res Commun 430:1301-5. 2013
    ..Present results highlight the potential role of MaPgb in scavenging of reactive nitrogen and oxygen species...
  14. doi request reprint Reactivity of the human hemoglobin "dark side"
    Paolo Ascenzi
    Laboratorio Interdipartimentale di Microscopia Elettronica, Universita Roma Tre, Roma, Italy
    IUBMB Life 65:121-6. 2013
    ....
  15. doi request reprint Cyanide binding to human plasma heme-hemopexin: a comparative study
    Paolo Ascenzi
    Laboratorio Interdipartimentale di Microscopia Elettronica, Universita Roma Tre, Roma, Italy
    Biochem Biophys Res Commun 428:239-44. 2012
    ..9 ± 0.8 M(-1/2) s(-1). HHPX-heme-Fe reactivity is modulated by proton acceptor/donor amino acid residue(s) (e.g., His236) assisting the deprotonation and protonation of the incoming and outgoing ligand, respectively...
  16. doi request reprint Pseudo-enzymatic hydrolysis of 4-nitrophenyl acetate by human serum albumin: pH-dependence of rates of individual steps
    Paolo Ascenzi
    Interdepartmental Laboratory of Electron Microscopy, University Roma Tre, Via della Vasca Navale 79, I 00146 Roma, Italy
    Biochem Biophys Res Commun 424:451-5. 2012
    ..0 ± 0.1 in the substrate-free HSA to 8.1 ± 0.1 in the HSA:NphOAc complex. Accordingly, diazepam inhibits competitively the HSA-catalyzed hydrolysis of NphOAc by binding to Tyr411...
  17. doi request reprint Pseudo-enzymatic hydrolysis of 4-nitrophenyl myristate by human serum albumin
    Paolo Ascenzi
    Interdepartmental Laboratory of Electron Microscopy, University Roma Tre, Via della Vasca Navale 79, I 00146 Roma, Italy
    Biochem Biophys Res Commun 422:219-23. 2012
    ..9 ± 0.2 in NphOMy-free HSA to 6.8 ± 0.3 in the HSA:NphOMy adduct. The HSA-catalyzed hydrolysis of NphOMy is inhibited competitively by diazepam, indicating that Tyr411 is the active-site nucleophile...
  18. ncbi request reprint Agmatine oxidation by copper amine oxidase
    Paolo Ascenzi
    Department of Biology, University Roma Tre, Rome, Italy
    Eur J Biochem 269:884-92. 2002
    ..0 x 10(-8) m) from specific binding sites in heart rat membranes, values of IC50 being (1.3 +/- 0.4) x 10(-9) m and (2.2 +/- 0.4) x 10(-8) m, respectively (at pH 7.4 and 37.0 degrees C)...
  19. ncbi request reprint Can onions be engineered for not tearing?
    Paolo Ascenzi
    IUBMB Life 55:49-50. 2003
  20. ncbi request reprint The bovine basic pancreatic trypsin inhibitor (Kunitz inhibitor): a milestone protein
    Paolo Ascenzi
    Dipartimento di Biologia, Universita Roma Tre, Italy
    Curr Protein Pept Sci 4:231-51. 2003
    ..Here, the structural, inhibition, and bio-medical aspects of BPTI are reported...
  21. ncbi request reprint The Rhodococcus sp. cocaine esterase: a bacterial candidate for novel pharmacokinetic-based therapies for cocaine abuse
    Paolo Ascenzi
    Department of Biology, University Roma Tre, Viale G Marconi 446, I 00146 Roma, Italy
    IUBMB Life 55:397-402. 2003
    ..The discovery of the cocaine esterase from the strain MBI of the bacterium Rhodococcus sp. (cocE) could be a major breakthrough in this field; cocE hydrolyzes cocaine faster than any known cocaine esterase and catalytic antibody...
  22. ncbi request reprint Question. Do NOS2 promoter polymorphisms protect against malaria?
    Paola Ascenzi
    IUBMB Life 56:229-30. 2004
  23. ncbi request reprint Inactivation of parasite cysteine proteinases by the NO-donor 4-(phenylsulfonyl)-3-((2-(dimethylamino)ethyl)thio)-furoxan oxalate
    Paolo Ascenzi
    Dipartimento di Biologia and Laboratorio Interdipartimentale di Microscopia Elettronica, Universita Roma Tre, Viale Guglielmo Marconi 446, I 00146 Roma, Italy
    Biochim Biophys Acta 1703:69-77. 2004
    ..infantum cysteine proteinase displays a maximum at about 340 nm. These results indicate that the parasite cysteine proteinase inactivation by SNO-102 occurs via the NO-mediated S-nitrosylation of the Cys25 catalytic residue...
  24. ncbi request reprint Proton-linked subunit heterogeneity in ferrous nitrosylated human adult hemoglobin: an EPR study
    Paolo Ascenzi
    Department of Biology, Interdepartmental Laboratory for Electron Microscopy, University Roma Tre, Viale Guglielmo Marconi 446, I 00146 Rome, Italy
    J Inorg Biochem 99:1255-9. 2005
    ..This probably reflects a 'quaternary effect', i.e., structural changes in both subunits upon tetrameric assembly, which is associated to a relevant variation of functional properties (i.e., proton affinity)...
  25. ncbi request reprint Heme impairs allosterically drug binding to human serum albumin Sudlow's site I
    Paolo Ascenzi
    National Institute for Infectious Diseases I R C C S Lazzaro Spallanzani, Via Portuense 292, I 00149 Roma, Italy
    Biochem Biophys Res Commun 334:481-6. 2005
    ..According to linked functions, drugs impair allosterically heme binding to HSA. These results appear to be relevant in the drug therapy and management...
  26. ncbi request reprint Thrombin inhibition by the highly selective 'reversible suicide substrate' N-ethoxycarbonyl-D-phenylalanyl-L-prolyl-alpha-azalysine p-nitrophenyl ester
    Paolo Ascenzi
    Department of Biology and Interdepartmental Laboratory for Electron Microscopy, University Roma Tre, Viale Marconi 446, I 00146 Roma, Italy
    Protein Pept Lett 12:433-8. 2005
    ..Eoc-D-Phe-Pro-azaLys-ONp is an acylating agent, but its hydrolysis product 1(N-ethoxycarbonyl-D-phenylalanyl-L-prolyl)-2(4-aminobutyl) hydrazine behaves as a highly selective human alpha-thrombin competitive inhibitor...
  27. ncbi request reprint How I became a biochemist
    Paolo Ascenzi
    Department of Biology, University Roma Tre, and National Institute for Infectious Diseases IRCCS Lazzaro Spallanzani, Roma, Italy
    IUBMB Life 57:599-601. 2005
  28. ncbi request reprint Do neuroglobin and myoglobin protect Toxoplasma gondii from nitrosative stress?
    Paolo Ascenzi
    Istituto Nazionale per le Malattie Infettive IRCCS Lazzaro Spallanzani, Roma, Italy
    IUBMB Life 57:689-91. 2005
    ..cruzi in cardiomyocytes, respectively, from the parasiticidal effect of NO. Therefore, undesirable pathogen protection by pseudo-enzymatic NO scavenging may represent a new unexpected function of members of the Hb superfamily...
  29. ncbi request reprint Allosteric modulation of drug binding to human serum albumin
    Paolo Ascenzi
    Istituto Nazionale per le Malattie Infettive I R C C S Lazzaro Spallanzani, Via Portuense 292, I 00149 Roma, Italy
    Mini Rev Med Chem 6:483-9. 2006
    ..Binding of exogenous and endogenous ligands to HSA appears to be relevant in drug therapy and management. Here, the allosteric modulation of drug binding to HSA is briefly reviewed...
  30. ncbi request reprint CO2 impairs peroxynitrite-mediated inhibition of human caspase-3
    Paolo Ascenzi
    Department of Biology, University Roma Tre, Viale Guglielmo Marconi 446, I 00146 Roma, Italy
    Biochem Biophys Res Commun 349:367-71. 2006
    ..These results represent the first evidence for modulation of peroxynitrite-mediated inhibition of cysteine proteinase action by CO2, supporting the role of CO2 in fine tuning of cell processes (e.g., apoptosis)...
  31. ncbi request reprint Abacavir modulates peroxynitrite-mediated oxidation of ferrous nitrosylated human serum heme-albumin
    Paolo Ascenzi
    Department of Biology, University Roma Tre, Viale Guglielmo Marconi 446, I 00146 Roma, Italy
    Biochem Biophys Res Commun 353:469-74. 2007
    ..8+/-0.3) x 10(-1) s(-1)) is CO2- and abacavir-independent, representing the rate-limiting step. Present data represent the first evidence for the allosteric modulation of SA-heme reactivity by heterotropic interaction(s)...
  32. ncbi request reprint Mycobacterial truncated hemoglobins: from genes to functions
    Paolo Ascenzi
    National Institute for Infectious Diseases I R C C S Lazzaro Spallanzani, Via Portuense 292, I 00149 Roma, Italy
    Gene 398:42-51. 2007
    ..Here, recent genomic, structural and biochemical information on mycobacterial trHbs is reviewed, with the aim of providing further insights into the role of these globins in mycobacterial physiology...
  33. doi request reprint H2O2 and (.)NO scavenging by Mycobacterium leprae truncated hemoglobin O
    Paolo Ascenzi
    Department of Biology and Interdepartmental Laboratory for Electron Microscopy, University Roma Tre, Viale Guglielmo Marconi 446, I 00146 Roma, Italy
    Biochem Biophys Res Commun 373:197-201. 2008
    ..Therefore, Mycobacterium leprae trHbO could be involved in both H2O2 and (.)NO scavenging, protecting from nitrosative and oxidative stress, and sustaining mycobacterial respiration...
  34. doi request reprint Peroxynitrite detoxification by ferryl Mycobacterium leprae truncated hemoglobin O
    Paolo Ascenzi
    Department of Biology and Interdepartmental Laboratory for Electron Microscopy, University Roma Tre, Viale Guglielmo Marconi 446, I 00146 Roma, Italy
    Biochem Biophys Res Commun 380:392-6. 2009
    ..As a whole, Ml-trHbO can undertake within the same cycle H(2)O(2) and peroxynitrite detoxification...
  35. pmc Ibuprofen impairs allosterically peroxynitrite isomerization by ferric human serum heme-albumin
    Paolo Ascenzi
    Department of Biology and Interdepartmental Laboratory for Electron Microscopy, University Roma Tre, I 00146 Roma, Italy
    J Biol Chem 284:31006-17. 2009
    ..The present results could be relevant for the drug-dependent protective role of HSA-heme-Fe(III) in vivo...
  36. doi request reprint Peroxynitrite scavenging by ferryl sperm whale myoglobin and human hemoglobin
    Paolo Ascenzi
    Department of Biology and Interdepartmental Laboratory for Electron Microscopy, University Roma Tre, I 00146 Roma, Italy
    Biochem Biophys Res Commun 390:27-31. 2009
    ..1 x 10(4)M(-1)s(-1) and 1.2 x 10(5)M(-1)s(-1), respectively. As a whole, MbFe(IV)O and HbFe(IV)O, obtained by treatment with H(2)O(2), undertake within the same cycle H(2)O(2) and peroxynitrite detoxification...
  37. doi request reprint Serum heme-albumin: an allosteric protein
    Paolo Ascenzi
    Department of Biology and Interdepartmental Laboratory for Electron Microscopy, University Roma Tre, Viale Guglielmo Marconi 446, Rome, Italy
    IUBMB Life 61:1118-22. 2009
    ..Heme binding to SA and SA-heme reactivity are allosterically and competitively modulated by endogenous and exogenous third components, this being relevant in pharmacotherapy management...
  38. doi request reprint Reductive nitrosylation of ferric cyanide horse heart myoglobin is limited by cyanide dissociation
    Paolo Ascenzi
    Department of Biology and Interdepartmental Laboratory for Electron Microscopy, University Roma Tre, Roma, Italy
    Biochem Biophys Res Commun 393:196-200. 2010
    ..The k(off) value obtained experimentally matches very well to that calculated from values of the second-order rate constant (k(on)) and of the dissociation equilibrium constant (K) for cyanide binding to Mb(III) (k(off)=k(on)xK)...
  39. doi request reprint Allostery in a monomeric protein: the case of human serum albumin
    Paolo Ascenzi
    Department of Biology, University Roma Tre, Rome, Italy
    Biophys Chem 148:16-22. 2010
    ..Here, both functional and structural aspects of the allosteric modulation of heme and drug (e.g., warfarin and ibuprofen) binding to HSA and of the drug-dependent reactivity of HSA-heme are reviewed...
  40. doi request reprint Peroxynitrite-An ugly biofactor?
    Paolo Ascenzi
    Department of Biology, University Roma Tre, Italy
    Biofactors 36:264-73. 2010
    ..g., hemoglobin and myoglobin). Here, we report biochemical aspects of peroxynitrite actions being at the root of its biomedical effects...
  41. doi request reprint Isoniazid and rifampicin inhibit allosterically heme binding to albumin and peroxynitrite isomerization by heme-albumin
    Paolo Ascenzi
    Department of Biology and Interdepartmental Laboratory for Electron Microscopy, University Roma Tre, Viale Guglielmo Marconi 446, Rome, Italy
    J Biol Inorg Chem 16:97-108. 2011
    ..These results highlight the role of drugs in modulating heme-Fe(III) binding to HSA and HSA-heme-Fe(III) reactivity...
  42. doi request reprint Cytochromes: Reactivity of the "dark side" of the heme
    Paolo Ascenzi
    Department of Biology, University Roma Tre, Viale Guglielmo Marconi 446, I 00146 Roma, Italy
    Biophys Chem 152:21-7. 2010
    ..Here, structural and functional aspects of NO binding to ferrous Axcyt c' and (CL-)hhcyt c are reviewed...
  43. doi request reprint Drug binding to Sudlow's site I impairs allosterically human serum heme-albumin-catalyzed peroxynitrite detoxification
    Paolo Ascenzi
    Department of Biology and Interdepartmental Laboratory for Electron Microscopy, University Roma Tre, Roma, Italy
    IUBMB Life 62:776-80. 2010
    ..e., the fatty acid site 1, FA1), depending on ligand occupancy of either sites...
  44. doi request reprint Cardiolipin modulates allosterically peroxynitrite detoxification by horse heart cytochrome c
    Paolo Ascenzi
    Department of Biology and Interdepartmental Laboratory for Electron Microscopy, University Roma Tre, I 00146 Roma, Italy
    Biochem Biophys Res Commun 404:190-4. 2011
    ..1±0.8)×10(-5) M. These results suggest that CL-cytc could play either pro-apoptotic or anti-apoptotic effects facilitating lipid peroxidation and scavenging of reactive nitrogen species, such as peroxynitrite, respectively...
  45. doi request reprint O2-mediated oxidation of ferrous nitrosylated human serum heme-albumin is limited by nitrogen monoxide dissociation
    Paolo Ascenzi
    Interdepartmental Laboratory of Electron Microscopy, University Roma Tre, Via della Vasca Navale 79, I 00146 Roma, Italy
    Biochem Biophys Res Commun 406:112-6. 2011
    ..These results reflect the fast O(2)-mediated oxidation of ferrous HSA-heme-Fe and highlight the role of drugs in modulating allosterically the heme-Fe-atom reactivity...
  46. doi request reprint Cardiolipin drives cytochrome c proapoptotic and antiapoptotic actions
    Paolo Ascenzi
    Interdepartmental Laboratory for Electron Microscopy, Via della Vasca Navale 79, Roma, Italy
    IUBMB Life 63:160-5. 2011
    ..e., inhibiting lipid peroxidation and hhcytc traslocation). Here, the CL-driven allosteric modulation of hhcytc properties is reviewed, highlighting proapoptotic and antiapoptotic actions..
  47. doi request reprint Allosteric modulation of monomeric proteins*
    Paolo Ascenzi
    Dipartimento di Biologia and Laboratorio Interdipartimentale di Microscopia Elettronica, Universita Roma Tre, Viale Guglielmo Marconi 446, I 00146 Roma, Italy Istituto Nazionale per le Malattie Infettive I R C C S Lazzaro Spallanzani, Via Portuense 292, I 00149 Roma, Italy
    Biochem Mol Biol Educ 33:169-76. 2005
    ..Here, the allosteric properties of sperm whale myoglobin, human serum albumin, and human α-thrombin, generally considered as molecular models of monomeric proteins, are summarized...
  48. doi request reprint Ibuprofen and warfarin modulate allosterically ferrous human serum heme-albumin nitrosylation
    Paolo Ascenzi
    Department of Biology and Interdepartmental Laboratory for Electron Microscopy, University Roma Tre, Via della Vasca Navale 79, I 00146 Roma, Italy
    Biochem Biophys Res Commun 411:185-9. 2011
    ..Present data highlight the allosteric modulation of HSA-heme-Fe(II) reactivity by heterotropic effectors...
  49. doi request reprint Peroxynitrite detoxification by horse heart carboxymethylated cytochrome c is allosterically modulated by cardiolipin
    Paolo Ascenzi
    Department of Biology and Interdepartmental Laboratory for Electron Microscopy, University Roma Tre, I 00146 Roma, Italy
    Biochem Biophys Res Commun 415:463-7. 2011
    ..1 × 10(-5)M). Although CM-cytc-Fe(III) and CL-cytc-Fe(III) display a different heme distal geometry and heme-Fe(III) reactivity, the heme pocket and the CL cleft are allosterically linked...
  50. ncbi request reprint Heme-hemopexin: a 'chronosteric' heme-protein
    Paolo Ascenzi
    Interdepartmental Laboratory for Electron Microscopy, University Roma Tre, Roma, Italy
    IUBMB Life 59:700-8. 2007
    ..HPX, acting not only as a heme carrier but also displaying transient heme-based ligand binding and (pseudo-)enzymatic properties, could be considered a 'chronosteric' heme-protein...
  51. ncbi request reprint Clonidine displacement from type 1 imidazoline receptor by p-aminobenzamidine, the prototype of trypsin-like serine protease inhibitors
    Valentina Pallottini
    Department of Biology, University Roma Tre, Viale G Marconi 446, 1 00146 Roma, Italy
    IUBMB Life 54:301-4. 2002
    ..Therefore, trypsin-like serine protease inhibitors structurally related to p-aminobenzamidine should be administrated under careful control...
  52. doi request reprint Reductive nitrosylation of ferric human serum heme-albumin
    Paolo Ascenzi
    Department of Biology, University Roma Tre, Roma, Italy
    FEBS J 277:2474-85. 2010
    ..4 x 10(3) M(-1) s(-1). The present results highlight the role of HSA-heme-Fe in scavenging reactive nitrogen species...
  53. doi request reprint Ferrous Campylobacter jejuni truncated hemoglobin P displays an extremely high reactivity for cyanide - a comparative study
    Alessandro Bolli
    Dipartimento di Biologia, Universita Roma Tre, Italy
    FEBS J 275:633-45. 2008
    ..The very high affinity of cyanide for Cj-trHbP(II), reminiscent of that of horseradish peroxidase(II), suggests that this globin may participate in cyanide detoxification...
  54. ncbi request reprint Expression of L-ornithine Ndelta-oxygenase (PvdA) in fluorescent Pseudomonas species: an immunochemical and in silico study
    Lorenza Putignani
    Unità di Microbiologia Molecolare, Istituto Nazionale per le Malattie Infettive I R C C S Lazzaro Spallanzani, Via Portuense 292, 00149 Roma, Italy
    Biochem Biophys Res Commun 313:245-57. 2004
    ..The MAb library was also used to monitor PvdA expression during the transition of P. aeruginosa from iron-sufficient to iron-deficient growth...
  55. ncbi request reprint Inhibition of polyamine and spermine oxidases by polyamine analogues
    Marzia Bianchi
    Dipartimento di Biologia, Universita Roma Tre, Italy
    FEBS J 273:1115-23. 2006
    ..Thus, the data reported here provide the basis for the development of novel and selective inhibitors able to discriminate between mammalian SMO and PAO activities...
  56. doi request reprint Scavenging of reactive nitrogen species by mycobacterial truncated hemoglobins
    Paolo Ascenzi
    National Institute for Infectious Diseases IRCCS Lazzaro Spallanzani, Rome, Italy
    Methods Enzymol 436:317-37. 2008
    ..Here, mechanisms for scavenging of reactive nitrogen species by mycobacterial trHbs are reviewed, and detailed protocols for assessing pseudoenzymatic kinetics are provided...
  57. ncbi request reprint Molecular bases for the anti-parasitic effect of NO (Review)
    Marco Colasanti
    Dipartimento di Biologia, Universita Roma Tre, I 00146 Rome, Italy
    Int J Mol Med 9:131-4. 2002
    ..g., cysteine proteases) appears to be a common and widespread mechanism. This overview concerns parasitic cysteine proteases as NO targets, providing molecular bases for the parasiticidal effect of NO...
  58. ncbi request reprint S-palmitoylation modulates human estrogen receptor-alpha functions
    Filippo Acconcia
    Department of Biology, University Roma Tre, Viale G Marconi, 446, I 00146, Rome, Italy
    Biochem Biophys Res Commun 316:878-83. 2004
    ..e., ligand binding domain). These results point to the pivotal role of the Cys447 residue in ERalpha palmitoylation and in the modulation of E2-induced non-genomic functions...
  59. pmc Palmitoylation-dependent estrogen receptor alpha membrane localization: regulation by 17beta-estradiol
    Filippo Acconcia
    Department of Biology, University Roma Tre, I 00146 Rome, Italy
    Mol Biol Cell 16:231-7. 2005
    ..These data point to the physiological role of ERalpha palmitoylation in the receptor localization to the cell membrane and in the regulation of the E2-induced cell proliferation...
  60. ncbi request reprint Binding of anti-HIV drugs to human serum albumin
    Alessio Bocedi
    National Institute for Infectious Diseases IRCCS Lazzaro Spallanzani, Via Portuense 292, I 00149 Rome, Italy
    IUBMB Life 56:609-14. 2004
    ....
  61. ncbi request reprint Allosteric modulation of anti-HIV drug and ferric heme binding to human serum albumin
    Alessio Bocedi
    National Institute for Infectious Diseases I R C C S Lazzaro Spallanzani, Roma, Italy
    FEBS J 272:6287-96. 2005
    ..In turn, anti-HIV drugs allosterically impair heme binding to HSA. Therefore, Sudlow's site I and the heme cleft must be functionally linked...
  62. ncbi request reprint Truncated hemoglobin GlbO from Mycobacterium leprae alleviates nitric oxide toxicity
    Giulia Fabozzi
    National Institute for Infectious Diseases IRCCS Lazzaro Spallanzani, Via Portuense 292, I 00149 Rome, Italy
    Microb Pathog 40:211-20. 2006
    ..These activities would help to sustain the obligate aerobic metabolism required for intracellular survival of leprosy bacilli...
  63. doi request reprint Abacavir and warfarin modulate allosterically kinetics of NO dissociation from ferrous nitrosylated human serum heme-albumin
    Paolo Ascenzi
    Department of Biology and Interdepartmental Laboratory for Electron Microscopy, University Roma Tre, Viale Guglielmo Marconi 446, I 00146 Roma, Italy
    Biochem Biophys Res Commun 369:686-91. 2008
    ..This event parallels the stabilization of the six-coordinate derivative of the HSA-heme-Fe(II)-NO atom. Present data highlight the allosteric modulation of HSA-heme-Fe(II) reactivity by heterotropic effectors...
  64. doi request reprint Ibuprofen modulates allosterically NO dissociation from ferrous nitrosylated human serum heme-albumin by binding to three sites
    Paolo Ascenzi
    Department of Biology and Interdepartmental Laboratory for Electron Microscopy, University Roma Tre, Via della Vasca Navale 79, Rome, Italy
    Biochem Biophys Res Commun 387:83-6. 2009
    ....
  65. ncbi request reprint Nitric oxide limits parasite development in vectors and in invertebrate intermediate hosts
    Paolo Ascenzi
    Dipartimento di Biologia, Universita Roma Tre, Italy
    IUBMB Life 53:121-3. 2002
    ..Therefore, NO limits Trypanosoma, Plasmodium, and Schistosoma development at all stages of the parasite life cycle...
  66. ncbi request reprint The truncated hemoglobin from Mycobacterium leprae
    Paolo Visca
    Dipartimento di Biologia e Laboratorio Interdipartimentale di Microscopia Elettronica, Universita Roma Tre, Viale G Marconi 446, I 00146 Rome, Italy
    Biochem Biophys Res Commun 294:1064-70. 2002
    ..7 x 10(6) M(-1) s(-1)). The lower reactivity of M. leprae trHbO as compared to M. tuberculosis trHbN might be related to the higher susceptibility of the leprosy bacillus to toxic nitrogen and oxygen species produced by phagocytic cells...
  67. ncbi request reprint Anthrax toxin: a tripartite lethal combination
    Paolo Ascenzi
    Department of Biology and Interdepartmental Laboratory for Electron Microscopy, University Roma Tre, Viale G Marconi 446, 00146, Rome, Italy
    FEBS Lett 531:384-8. 2002
    ..Inhibitors targeting different steps of toxin activity have recently been developed. Anthrax toxin has also been exploited as a therapeutic agent against cancer...
  68. ncbi request reprint Do hemoglobin and hemocyanin impair schistosoma killing by no?
    Paolo Ascenzi
    Dipartimento di Biologia, Universita Roma Tre, Roma, Italy
    IUBMB Life 53:287-8. 2002
    ..Interestingly, hemoglobin, myoglobin, and neuroglobin may protect Plasmodium and Trypanosoma from the antiparasitic effects of NO...
  69. ncbi request reprint Is there evidence that H2S (like CO and NO) may be a neuromodulator? If it is, what is the mechanism of its action?
    Paolo Ascenzi
    IUBMB Life 54:371-2. 2002
  70. ncbi request reprint Are specific hydrolases bioscavengers for defense against organophosphorus nerve agents?
    A Alessio Bocedi
    IUBMB Life 55:491-2. 2003
  71. ncbi request reprint Estrogen receptor signalling: bases for drug actions
    Maria Marino
    Department of Biology, University Roma Tre, Viale G Marconi, 446, I 00146 Roma, Italy
    Curr Drug Targets Immune Endocr Metabol Disord 5:305-14. 2005
    ..This review will focus and summarize the latest findings regarding the multiple E2 molecular mechanisms and underlines the development of our understanding of anti-cancer drugs acting as ER signalling modulators...
  72. ncbi request reprint Nitric oxide scavenging by Mycobacterium leprae GlbO involves the formation of the ferric heme-bound peroxynitrite intermediate
    Paolo Ascenzi
    National Institute for Infectious Diseases, I R C C S Lazzaro Spallanzani, Via Portuense 292, I 00149 Rome, Italy
    Biochem Biophys Res Commun 339:450-6. 2006
    ....
  73. ncbi request reprint Do steroid hormones function via multiple signaling pathways?
    Maria Marino
    Department of Biology, University Roma Tre, Italy
    IUBMB Life 57:825-7. 2005
  74. ncbi request reprint Does CO(2) modulate peroxynitrite specificity?
    Paolo Ascenzi
    National Institute for Infectious Diseases IRCCS Lazzaro Spallanzani, Roma, Italy
    IUBMB Life 58:611-3. 2006
  75. ncbi request reprint Peroxynitrite scavenging by ferrous truncated hemoglobin GlbO from Mycobacterium leprae
    Paolo Ascenzi
    National Institute for Infectious Diseases I R C C S Lazzaro Spallanzani, Via Portuense 292, I 00149 Roma, Italy
    Biochem Biophys Res Commun 351:528-33. 2006
    ..5+/-0.2)x10(4)M(-1)s(-1)) is CO(2)-independent. These data argue for a role of GlbO in the defense of M. leprae against nitrosative stress...
  76. ncbi request reprint Reductive nitrosylation and peroxynitrite-mediated oxidation of heme-hemopexin
    Paolo Ascenzi
    Department of Biology and Interdepartmental Laboratory for Electron Microscopy, University Roma Tre, Rome, Italy
    FEBS J 274:551-62. 2007
    ..HPX-heme(II)-NO appears to act as an efficient scavenger of peroxynitrite and of strong oxidants and nitrating species following the reaction of peroxynitrite with CO2 (e.g. ONOOC(O)O-, CO3-, and NO2)...
  77. ncbi request reprint Steroid hormone rapid signaling: the pivotal role of S-palmitoylation
    Maria Marino
    Dipartimento di Biologia, Universittà Roma Tre, Roma, Italy
    IUBMB Life 58:716-9. 2006
    ..Reversible S-palmitoylation of SHRs could play a critical role in receptor localization at the plasma membrane and in rapid nongenomic signaling activation...
  78. ncbi request reprint *NO dissociation represents the rate limiting step for O2-mediated oxidation of ferrous nitrosylated Mycobacterium leprae truncated hemoglobin O
    Paolo Ascenzi
    Department of Biology and Interdepartmental Laboratory for Electron Microscopy, University Roma Tre, Viale Guglielmo Marconi 446, I 00146 Roma, Italy
    Biochem Biophys Res Commun 357:809-14. 2007
    ..leprae trHbO(III). *NO dissociation from heme(II)-NO represents the rate limiting step for O(2)-mediated oxidation of M. leprae trHbO(II)-NO...
  79. ncbi request reprint Common themes and variations in the rhodanese superfamily
    Rita Cipollone
    Dipartimento di Biologia, Universita Roma Tre, Roma, Italy
    IUBMB Life 59:51-9. 2007
    ....
  80. ncbi request reprint Ferricyanide-mediated oxidation of ferrous nitrosylated sperm whale myoglobin involves the formation of the ferric nitrosylated intermediate
    Paolo Ascenzi
    Department of Biology and Interdepartmental Laboratory for Electron Microscopy, University Roma Tre, Viale Guglielmo Marconi 446, I 00146 Roma, Italy
    Biochem Biophys Res Commun 359:871-6. 2007
    ..3s(-1), respectively, at pH 8.3 and 20.0 degrees C. Since ()NO dissociation from Mb(II)-NO is very slow, and (unlike O(2) and CO) ()NO is a ligand for both Mb(II) and Mb(III), Mb(II)-NO can be oxidized without requiring ()NO dissociation...
  81. doi request reprint Membrane association of estrogen receptor alpha and beta influences 17beta-estradiol-mediated cancer cell proliferation
    Maria Marino
    Department of Biology, University Roma Tre, Viale G Marconi 446, I 00146 Roma, Italy
    Steroids 73:853-8. 2008
    ....
  82. doi request reprint The nutritional flavanone naringenin triggers antiestrogenic effects by regulating estrogen receptor alpha-palmitoylation
    Paola Galluzzo
    Department of Biology, University Roma Tre, Viale G Marconi, 446, I 00146 Roma, Italy
    Endocrinology 149:2567-75. 2008
    ..Moreover, the different modulation of ERalpha palmitoylation exerted by different ligands represents a pivotal mechanism that drives cancer cell to proliferation or apoptosis...
  83. ncbi request reprint Catalytic properties of cysteine proteinases from Trypanosoma cruzi and Leishmania infantum: a pre-steady-state and steady-state study
    Paolo Ascenzi
    Dipartimento di Biologia, Universita Roma Tre, Viale Guglielmo Marconi 446, I 00146 Rome, Italy
    Biochem Biophys Res Commun 309:659-65. 2003
    ..Comparison with the steady-state and pre-steady-state kinetics of homologous plant enzymes suggests that the parasite cysteine proteinase catalytic behavior appears to be of general significance...
  84. ncbi request reprint The anti-parasitic effects of nitric oxide
    Paolo Ascenzi
    Dipartimento di Biologia, Universita Roma Tre, Viale G Marconi 446, 1 00146 Roma, Italy
    IUBMB Life 55:573-8. 2003
    ....
  85. ncbi request reprint Analytical and pharmacological aspects of therapeutic drug monitoring of mTOR inhibitors
    Maria Pieri
    Department of Public Medicine and Social Safety University Federico II of Naples Via S Pansini 5, I 80131, Naples, Italy
    Curr Drug Metab 12:253-67. 2011
    ..The aim of the present paper is to report the most recent knowledge concerning this issue, supplying a critical and comprehensive review for whom are involved both in the clinical and analytical areas...
  86. ncbi request reprint Xenosensors CAR and PXR at work: impact on statin metabolism
    Maria Marino
    Department of Biology, University Roma Tre, Viale Guglielmo Marconi 446, I 00146 Roma, Italy
    Curr Drug Metab 12:300-11. 2011
    ..Moreover, the alteration of statin pharmacodynamics and/or pharmacokinetics induced by concomitant drug treatment or dietary factors will be also examined...
  87. ncbi request reprint The 109 residue nerve tissue minihemoglobin from Cerebratulus lacteus highlights striking structural plasticity of the alpha-helical globin fold
    Alessandra Pesce
    Department of Physics, INFM, Advanced Biotechnology Centre, University of Genova, Largo Rosanna Benzi 10, I 16146 Genova, Italy
    Structure 10:725-35. 2002
    ..Ligand access to heme may take place through a wide protein matrix tunnel connecting the distal site to a surface cleft located between the E and H helices...
  88. ncbi request reprint The heme-iron geometry of ferrous nitrosylated heme-serum lipoproteins, hemopexin, and albumin: a comparative EPR study
    Mauro Fasano
    Department of Structural and Functional Biology, University of Insubria, via Jean H Dunant 3, I 21100 Varese, Italy
    J Inorg Biochem 91:487-90. 2002
    ..Altogether, these observations suggest that HPX displays a cleft much more suitable for heme binding than other heme-carriers...
  89. pmc Neuroglobin and cytoglobin. Fresh blood for the vertebrate globin family
    Alessandra Pesce
    Department of Physics INFM and Center for Excellence in Biomedical Research, University of Genova, Via Dodecaneso 33, I 16146 Genova, Italy
    EMBO Rep 3:1146-51. 2002
    ..Strikingly, the data reveal that O2 metabolism in cells is more complicated than was thought before, requiring unexpected O2-binding proteins with potentially novel functional features...
  90. ncbi request reprint Relationships of ligand binding, redox properties, and protonation in Coprinus cinereus peroxidase
    Chiara Ciaccio
    Department of Experimental Medicine and Biochemical Sciences, Universita di Roma Tor Vergata, Via Montpellier 1, I 00133 Roma, Italy
    J Biol Chem 278:18730-7. 2003
    ....
  91. ncbi request reprint AtCYS1, a cystatin from Arabidopsis thaliana, suppresses hypersensitive cell death
    Beatrice Belenghi
    Dipartimento Scientifico e Tecnologico, Universita degli Studi di Verona, Verona, Italy
    Eur J Biochem 270:2593-604. 2003
    ....
  92. ncbi request reprint Inhibition of Azotobacter vinelandii rhodanese by NO-donors
    Andrea Spallarossa
    Dipartimento di Scienze Farmaceutiche, Universita di Genova, Via Benedetto XV 3, I 16132 Genoa, Italy
    Biochem Biophys Res Commun 306:1002-7. 2003
    ..These results indicate that RhdA inhibition occurs via NO-mediated S-nitrosylation of the unique Cys230 catalytic residue...
  93. ncbi request reprint The metalloproteolytic activity of the anthrax lethal factor is substrate-inhibited
    Fiorella Tonello
    Istituto di Neuroscienze, Consiglio Nazionale delle Ricerche, Via G Colombo 3, 35121 Padova, Italy
    J Biol Chem 278:40075-8. 2003
    ..LF undergoes substrate inhibition, in keeping with the non-productive binding geometry of the MAPPK-2 N terminus to LF...
  94. ncbi request reprint Human brain neuroglobin structure reveals a distinct mode of controlling oxygen affinity
    Alessandra Pesce
    Department of Physics INFM and Centre for Excellence in Biomedical Research, University of Genova, Via Dodecaneso 33, I 16146 Genova, Italy
    Structure 11:1087-95. 2003
    ....
  95. ncbi request reprint Kinetics of parasite cysteine proteinase inactivation by NO-donors
    Alessio Bocedi
    Laboratorio di Parassitologia, Istituto Superiore di Sanita, Viale Regina Elena 299, I 00161 Rome, Italy
    Biochem Biophys Res Commun 315:710-8. 2004
    ..infantum cysteine proteinase from inactivation by SNAP. These results indicate that parasite cysteine proteinase inactivation by NO-donors occurs via NO-mediated S-nitrosylation of the Cys25 catalytic residue...
  96. ncbi request reprint Mapping protein matrix cavities in human cytoglobin through Xe atom binding
    Daniele de Sanctis
    Department of Physics INFM and Centre for Excellence in Biomedical Research, University of Genova, Via Dodecaneso 33, Genoa I 16146, Italy
    Biochem Biophys Res Commun 316:1217-21. 2004
    ..Despite the conserved globin fold, the cavity found in CYGB* is structured differently from those recognized to play a functional role in myoglobin, neuroglobin, truncated hemoglobins, and Cerebratulus lacteus mini-hemoglobin...
  97. ncbi request reprint Crystal structure of cytoglobin: the fourth globin type discovered in man displays heme hexa-coordination
    Daniele de Sanctis
    Department of Physics INFM, Centre for Excellence in Biomedical Research, University of Genova, Via Dodecaneso 33, 1 16146 Genova, Italy
    J Mol Biol 336:917-27. 2004
    ..The cavity, which may provide a heme ligand diffusion pathway, is connected to the external space through a narrow tunnel nestled between the globin G and H helices...
  98. ncbi request reprint Cyanide binding to truncated hemoglobins: a crystallographic and kinetic study
    Mario Milani
    Istituto Giannina Gaslini, Largo G Gaslini, 5 16147 Genova, Italy
    Biochemistry 43:5213-21. 2004
    ....
  99. ncbi request reprint Thr-E11 regulates O2 affinity in Cerebratulus lacteus mini-hemoglobin
    Alessandra Pesce
    Department of Physics INFM and Center for Excellence in Biomedical Research, University of Genova, Via Dodecaneso 33, 16146 Genova, Italy
    J Biol Chem 279:33662-72. 2004
    ....
  100. ncbi request reprint Nitrosylation of rabbit ferrous heme-hemopexin
    Mauro Fasano
    Department of Structural and Functional Biology, University of Insubria, Via Alberto da Giussano 12, 21052 Busto Arsizio VA, Italy
    J Biol Inorg Chem 9:800-6. 2004
    ..5x10(-1) M sodium chloride. This result is in agreement with the effect of sodium chloride on rabbit HPX-heme stability. The present data have been analyzed in parallel with those of a related heme model compound and heme-protein systems...
  101. ncbi request reprint Structural bases for heme binding and diatomic ligand recognition in truncated hemoglobins
    Mario Milani
    Institute Giannina Gaslini, Largo Giannina Gaslini 5, 16147 Genova, Italy
    J Inorg Biochem 99:97-109. 2005
    ..e., O2, CO, NO, and cyanide), albeit with varying thermodynamic and kinetic parameters. Here, structural bases for heme binding and diatomic ligand recognition by trHbs are reviewed...