Giuseppina Li Pira

Summary

Country: Italy

Publications

  1. ncbi Helper function of cytolytic lymphocytes: switching roles in the immune response
    Giuseppina Li Pira
    Advanced Biotechnology Center, Genoa, Italy
    Eur J Immunol 37:66-77. 2007
  2. ncbi Lymphocyte proliferation specific for recall, CMV and HIV antigens in miniaturized and automated format
    Giuseppina Li Pira
    Department of Pediatric Hematology and Oncology, IRCCS Bambino Gesù Children Hospital, Rome, Italy
    J Immunol Methods 384:135-42. 2012
  3. doi Selective binding of CD4 and CD8 T-cells to antigen presenting cells for enrichment of CMV and HIV specific T-lymphocytes
    Giuseppina Li Pira
    Department of Pediatric Hematology and Oncology, Bambino Gesu Children Hospital, Rome, Italy
    J Immunol Methods 376:125-31. 2012
  4. pmc High throughput T epitope mapping and vaccine development
    Giuseppina Li Pira
    Laboratory of Cellular Immunology, Advanced Biotechnology Center, 16132 Genoa, Italy
    J Biomed Biotechnol 2010:325720. 2010
  5. doi Pathogen specific T-lymphocytes for the reconstitution of the immunocompromised host
    Giuseppina Li Pira
    Cellular Immunology Laboratory, Advanced Biotechnology Center, Largo Benzi 10, 16132 Genoa, Italy
    Curr Opin Immunol 21:549-56. 2009
  6. pmc Evaluation of antigen-specific T-cell responses with a miniaturized and automated method
    Giuseppina Li Pira
    Laboratory of Cellular Immunology, Advanced Biotechnology Center, Largo Benzi 10, 16132 Genoa, Italy
    Clin Vaccine Immunol 15:1811-8. 2008
  7. doi Positive selection and expansion of cytomegalovirus-specific CD4 and CD8 T cells in sealed systems: potential applications for adoptive cellular immunoreconstitution
    Giuseppina Li Pira
    Laboratory of Cellular Immunology, Advanced Biotechnology Center, G Gaslini Institute, Genoa, Italy
    J Immunother 31:762-70. 2008
  8. ncbi High throughput functional microdissection of pathogen-specific T-cell immunity using antigen and lymphocyte arrays
    Giuseppina Li Pira
    Cellular Immunology Unit, Advanced Biotechnology Center, Largo Benzi 10, 16132 Genoa, Italy
    J Immunol Methods 326:22-32. 2007
  9. ncbi Measurement of antigen specific immune responses: 2006 update
    Giuseppina Li Pira
    Viral Immunology, Advanced Biotechnology Center, Largo Benzi 10, 16132 Genoa, Italy
    Cytometry B Clin Cytom 72:77-85. 2007
  10. ncbi Human bone marrow stromal cells hamper specific interactions of CD4 and CD8 T lymphocytes with antigen-presenting cells
    Giuseppina Li Pira
    Advanced Biotechnology Center, Genoa, Italy
    Hum Immunol 67:976-85. 2006

Collaborators

Detail Information

Publications25

  1. ncbi Helper function of cytolytic lymphocytes: switching roles in the immune response
    Giuseppina Li Pira
    Advanced Biotechnology Center, Genoa, Italy
    Eur J Immunol 37:66-77. 2007
    ..If this possibility is proven also in vivo, we should revise some of our views on the pathogenesis of diseases in which CD8 cells are key players, such as in viral infections, graft rejection and GVHD...
  2. ncbi Lymphocyte proliferation specific for recall, CMV and HIV antigens in miniaturized and automated format
    Giuseppina Li Pira
    Department of Pediatric Hematology and Oncology, IRCCS Bambino Gesù Children Hospital, Rome, Italy
    J Immunol Methods 384:135-42. 2012
    ..This procedure is consistent with our policy to miniaturize assays for specific T-cell immunity, as we have already established for cytokine secretion assays...
  3. doi Selective binding of CD4 and CD8 T-cells to antigen presenting cells for enrichment of CMV and HIV specific T-lymphocytes
    Giuseppina Li Pira
    Department of Pediatric Hematology and Oncology, Bambino Gesu Children Hospital, Rome, Italy
    J Immunol Methods 376:125-31. 2012
    ....
  4. pmc High throughput T epitope mapping and vaccine development
    Giuseppina Li Pira
    Laboratory of Cellular Immunology, Advanced Biotechnology Center, 16132 Genoa, Italy
    J Biomed Biotechnol 2010:325720. 2010
    ..The currently used methods are described with special reference to the possibility of applying the HTP concept to make epitope mapping an easier procedure in terms of time, workload, reagents, cells and overall cost...
  5. doi Pathogen specific T-lymphocytes for the reconstitution of the immunocompromised host
    Giuseppina Li Pira
    Cellular Immunology Laboratory, Advanced Biotechnology Center, Largo Benzi 10, 16132 Genoa, Italy
    Curr Opin Immunol 21:549-56. 2009
    ..Finally, the development of biobanks of specific T-cells is described as an attractive perspective to reconstruct pathogen specific cellular immunity...
  6. pmc Evaluation of antigen-specific T-cell responses with a miniaturized and automated method
    Giuseppina Li Pira
    Laboratory of Cellular Immunology, Advanced Biotechnology Center, Largo Benzi 10, 16132 Genoa, Italy
    Clin Vaccine Immunol 15:1811-8. 2008
    ....
  7. doi Positive selection and expansion of cytomegalovirus-specific CD4 and CD8 T cells in sealed systems: potential applications for adoptive cellular immunoreconstitution
    Giuseppina Li Pira
    Laboratory of Cellular Immunology, Advanced Biotechnology Center, G Gaslini Institute, Genoa, Italy
    J Immunother 31:762-70. 2008
    ..Similar sealed protocols can also be applied for T-cell therapies directed against tumor antigens...
  8. ncbi High throughput functional microdissection of pathogen-specific T-cell immunity using antigen and lymphocyte arrays
    Giuseppina Li Pira
    Cellular Immunology Unit, Advanced Biotechnology Center, Largo Benzi 10, 16132 Genoa, Italy
    J Immunol Methods 326:22-32. 2007
    ..The assay provides quantitative information on cytokines induced by antigen stimulation and can be applied in a simplified format as a field test to monitor T-cell immunity in vaccine trials or in veterinary medicine...
  9. ncbi Measurement of antigen specific immune responses: 2006 update
    Giuseppina Li Pira
    Viral Immunology, Advanced Biotechnology Center, Largo Benzi 10, 16132 Genoa, Italy
    Cytometry B Clin Cytom 72:77-85. 2007
    ....
  10. ncbi Human bone marrow stromal cells hamper specific interactions of CD4 and CD8 T lymphocytes with antigen-presenting cells
    Giuseppina Li Pira
    Advanced Biotechnology Center, Genoa, Italy
    Hum Immunol 67:976-85. 2006
    ..In addition, if the reported suppressive effect of BMSCs on GVHD in vivo is confirmed, a different in vitro system should be envisaged to better understand and exploit the underlying mechanism...
  11. doi Validation of a miniaturized assay based on IFNg secretion for assessment of specific T cell immunity
    Giusi Li Pira
    Advanced Biotechnology Center, Largo Benzi 10, 16132, Genoa, Italy
    J Immunol Methods 355:68-75. 2010
    ....
  12. ncbi Recognition of CMV pp65 protein antigen by human CD4 T-cell lines induced with an immunodominant peptide pool
    Giuseppina Li Pira
    Laboratory of Clinical and Experimental Immunology, G Gaslini Institute, Genoa, Italy
    Hum Immunol 65:537-43. 2004
    ..Furthermore, these lines lost alloreactivity, suggesting that they can be generated from the allodonor for adoptive immunoreconstitution of stem cell graft recipients...
  13. ncbi A sealed and unbreached system for purification, stimulation, and expansion of cytomegalovirus-specific human CD4 and CD8 T lymphocytes
    Giuseppina Li Pira
    Advanced Biotechnology Center, Genoa, Italy
    Transfusion 46:2053-62. 2006
    ..In fact, both expertises are required to provide effective and safe cellular products. Because of in vitro manipulation, T-lymphocyte cultures are at risk of contamination even under good manufacturing procedure (GMP) conditions...
  14. ncbi Generation of cytomegalovirus (CMV)-specific CD4 T cell lines devoid of alloreactivity, by use of a mixture of CMV-phosphoprotein 65 peptides for reconstitution of the T helper repertoire
    Giuseppina Li Pira
    Laboratory of Clinical and Experimental Immunology, Viral Immunology Unit, G Gaslini Institute, Largo Gaslini 5, 16148 Genoa, Italy
    J Infect Dis 191:215-26. 2005
    ..Yet CD4 cells have been neglected, even though they contribute to the persistence of adoptively transferred CTLs...
  15. doi A registry of HLA-typed donors for production of virus-specific CD4 and CD8 T lymphocytes for adoptive reconstitution of immune-compromised patients
    Giuseppina Li Pira
    Bambino Gesu Children s Hospital, Rome, Italy
    Transfusion 54:3145-54. 2014
    ..Unfortunately, the graft donor is not always immune to the relevant pathogen or the graft donor may not be available (registry-derived or cord blood donors)...
  16. ncbi Identification of new Th peptides from the cytomegalovirus protein pp65 to design a peptide library for generation of CD4 T cell lines for cellular immunoreconstitution
    Giuseppina Li Pira
    Laboratory of Clinical and Experimental Immunology, G Gaslini Institute, Genoa 16147, Italy
    Int Immunol 16:635-42. 2004
    ....
  17. ncbi Human naive CD4 T-cell clones specific for HIV envelope persist for years in vivo in the absence of antigenic challenge
    Giuseppina Li Pira
    Laboratory of Clinical and Experimental Immunology, G Gaslini Institute, Genoa, Italy
    J Acquir Immune Defic Syndr 40:132-9. 2005
    ..An extended lifespan, homeostatic proliferation, or the ability of the thymus to issue the same CD4 T-cell clone reiteratively might account for the phenomenon...
  18. pmc Miniaturized and high-throughput assays for analysis of T-cell immunity specific for opportunistic pathogens and HIV
    Giuseppina Li Pira
    Bambino Gesu Children s Hospital, Rome, Italy
    Clin Vaccine Immunol 21:488-95. 2014
    ....
  19. doi Adoptive immunotherapy with antigen-specific T cells during extracorporeal membrane oxygenation (ECMO) for adenovirus-related respiratory failure in a child given haploidentical stem cell transplantation
    Matteo Di Nardo
    Department of Pediatric Intensive Care, IRCCS Bambino Gesù Children s Hospital, Rome, Italy
    Pediatr Blood Cancer 61:376-9. 2014
    ..T-cell responses to HAdV antigens were documented after four weeks and were associated with viral clearance, immune reconstitution and clinical amelioration...
  20. pmc Biological, functional and genetic characterization of bone marrow-derived mesenchymal stromal cells from pediatric patients affected by acute lymphoblastic leukemia
    Antonella Conforti
    Department of Pediatric Hematology Oncology, IRCCS Bambino Gesù Children s Hospital, Rome, Italy
    PLoS ONE 8:e76989. 2013
    ..Our findings indicate that ALL-MSCs display reduced ability to proliferate and to support long-term hematopoiesis in vitro. ALL-MSCs isolated at diagnosis do not differ from those obtained during treatment. ..
  21. pmc Mobilization of healthy donors with plerixafor affects the cellular composition of T-cell receptor (TCR)-αβ/CD19-depleted haploidentical stem cell grafts
    Sergio Rutella
    Department of Pediatric Hematology Oncology and Transfusion Medicine, IRCCS Bambino Gesù Children s Hospital, Rome, Italy
    J Transl Med 12:240. 2014
    ....
  22. doi Immunoselection techniques in hematopoietic stem cell transplantation
    Giuseppina Li Pira
    Department of Pediatric Hematology and Oncology, Unit of Immuno Hematology and Transfusion Medicine, IRCCS Bambino Gesù Children s Hospital, Rome, Italy Electronic address
    Transfus Apher Sci 54:356-63. 2016
    ..More complex manipulations, requiring a Good Manufacturing Practice (GMP) facility, are briefly mentioned. ..
  23. doi HLA-haploidentical stem cell transplantation after removal of αβ+ T and B cells in children with nonmalignant disorders
    Alice Bertaina
    Department of Pediatric Hematology and Oncology, Istituto di Ricovero e Cura a Carattere Scientifico Bambino Gesù Children s Hospital, Rome, Italy
    Blood 124:822-6. 2014
    ..Our data suggest that this novel graft manipulation strategy is safe and effective for haplo-HSCT. This trial was registered at www.clinicaltrials.gov as #NCT01810120. ..
  24. doi The PEDVAC trial: preliminary data from the first therapeutic DNA vaccination in HIV-infected children
    Paolo Palma
    Chair of Pediatrics, University of Rome Tor Vergata, Rome, Italy
    Vaccine 29:6810-6. 2011
    ..Evaluation of these results is in progress and will provide key information on the status and changes of antigen specific immunity during HIV DNA immunization...
  25. pmc TIM-3/Gal-9 interaction induces IFNγ-dependent IDO1 expression in acute myeloid leukemia blast cells
    Valentina Folgiero
    Department of Pediatric Hematology and Oncology, IRCCS Bambino Gesù Children s Hospital, Viale di San Paolo 15, 00146, Rome, Italy
    J Hematol Oncol 8:36. 2015
    ..Thus, the inhibition of TIM-3/Gal-9 immune check point, which affects NK cell-dependent IFNγ production and the consequent IDO1 activation, could usefully integrate current chemotherapeutic approaches. ..