Marina Cretich

Summary

Affiliation: Istituto di Chimica del Riconoscimento Molecolare
Country: Italy

Publications

  1. doi request reprint Flow-through, viral co-infection assay for resource-limited settings
    Marina Cretich
    Consiglio Nazionale delle Ricerche CNR, Istituto di Chimica del Riconoscimento Molecolare ICRM, Milano, Italy Electronic address
    Talanta 132:315-20. 2015
  2. doi request reprint Protein microarray technology: how far off is routine diagnostics?
    Marina Cretich
    Consiglio Nazionale delle Ricerche, Istituto di Chimica del Riconoscimento Molecolare ICRM, CNR, Via Mario Bianco, 9, 20131, Milano, Italy
    Analyst 139:528-42. 2014
  3. doi request reprint Detection of allergen specific immunoglobulins by microarrays coupled to microfluidics
    Marina Cretich
    Istituto di Chimica del Riconoscimento Molecolare ICRM, CNR, Milano, Italy
    Proteomics 9:2098-107. 2009
  4. doi request reprint Coating of nitrocellulose for colorimetric DNA microarrays
    Marina Cretich
    Istituto di Chimica del Riconoscimento Molecolare, CNR, 20131 Milano, Italy
    Anal Biochem 397:84-8. 2010
  5. doi request reprint Allergen microarrays on high-sensitivity silicon slides
    Marina Cretich
    Istituto di Chimica del Riconoscimento Molecolare ICRM, C N R, Via Mario Bianco, 9, 20131 Milan, Italy
    Anal Bioanal Chem 398:1723-33. 2010
  6. ncbi request reprint A new polymeric coating for protein microarrays
    Marina Cretich
    Istituto di Chimica del Riconoscimento Molecolare ICRM, C N R, Via Mario Bianco 9, 20131 Milano, Italy
    Anal Biochem 332:67-74. 2004
  7. ncbi request reprint Use of high-molecular-mass polyacrylamides as matrices for microchip electrophoresis of DNA fragments
    Marina Cretich
    Istituto di Chimica del Riconoscimento Molecolare, C N R, Milano, Italy
    Electrophoresis 24:3793-9. 2003
  8. doi request reprint Overcoming mass transport limitations to achieve femtomolar detection limits on silicon protein microarrays
    Marina Cretich
    Consiglio Nazionale delle Ricerche, Istituto di Chimica del Riconoscimento Molecolare, 20131 Milano, Italy
    Anal Biochem 418:164-6. 2011
  9. ncbi request reprint Protein and peptide arrays: recent trends and new directions
    Marina Cretich
    Istituto di Chimica del Riconoscimento Molecolare ICRM C N R, Via Mario Bianco, 9, 20131 Milano, Italy
    Biomol Eng 23:77-88. 2006
  10. doi request reprint Peptide microarrays on coated silicon slides for highly sensitive antibody detection
    Marina Cretich
    Istituto di Chimica del Riconoscimento Molecolare C N R, Milano, Italy
    Methods Mol Biol 669:147-60. 2010

Collaborators

Detail Information

Publications37

  1. doi request reprint Flow-through, viral co-infection assay for resource-limited settings
    Marina Cretich
    Consiglio Nazionale delle Ricerche CNR, Istituto di Chimica del Riconoscimento Molecolare ICRM, Milano, Italy Electronic address
    Talanta 132:315-20. 2015
    ....
  2. doi request reprint Protein microarray technology: how far off is routine diagnostics?
    Marina Cretich
    Consiglio Nazionale delle Ricerche, Istituto di Chimica del Riconoscimento Molecolare ICRM, CNR, Via Mario Bianco, 9, 20131, Milano, Italy
    Analyst 139:528-42. 2014
    ..A selection of microarray assays applicable to IVD and a summary of the diagnostic products currently available on the market are provided. ..
  3. doi request reprint Detection of allergen specific immunoglobulins by microarrays coupled to microfluidics
    Marina Cretich
    Istituto di Chimica del Riconoscimento Molecolare ICRM, CNR, Milano, Italy
    Proteomics 9:2098-107. 2009
    ..As a result of this, the binding of high affinity IgE antibodies is enhanced whereas that of low affinity IgG antibodies, which are present at higher concentration, is impaired paving the way to more accurate and sensitive results...
  4. doi request reprint Coating of nitrocellulose for colorimetric DNA microarrays
    Marina Cretich
    Istituto di Chimica del Riconoscimento Molecolare, CNR, 20131 Milano, Italy
    Anal Biochem 397:84-8. 2010
    ..The fast and robust procedure of nitrocellulose functionalization opens the opportunity to implement this material in disposable analytical microdevices that do not require sophisticated readout systems...
  5. doi request reprint Allergen microarrays on high-sensitivity silicon slides
    Marina Cretich
    Istituto di Chimica del Riconoscimento Molecolare ICRM, C N R, Via Mario Bianco, 9, 20131 Milan, Italy
    Anal Bioanal Chem 398:1723-33. 2010
    ..Moreover, silicon significantly enhanced the performance of the allergen microarray as compared to glass in accurately identifying allergic patients spanning a wide range of specific IgE titers to the considered allergens...
  6. ncbi request reprint A new polymeric coating for protein microarrays
    Marina Cretich
    Istituto di Chimica del Riconoscimento Molecolare ICRM, C N R, Via Mario Bianco 9, 20131 Milano, Italy
    Anal Biochem 332:67-74. 2004
    ..Moreover, in the RF assay, after hybridization with the sera, the slides have a low background, leading to a detection limit of 900amol/spot...
  7. ncbi request reprint Use of high-molecular-mass polyacrylamides as matrices for microchip electrophoresis of DNA fragments
    Marina Cretich
    Istituto di Chimica del Riconoscimento Molecolare, C N R, Milano, Italy
    Electrophoresis 24:3793-9. 2003
    ..Moreover, the low viscosity of the dilute solutions enable it to be easier and faster in filling the channel between runs, thus enhancing the throughput of the microchip devices...
  8. doi request reprint Overcoming mass transport limitations to achieve femtomolar detection limits on silicon protein microarrays
    Marina Cretich
    Consiglio Nazionale delle Ricerche, Istituto di Chimica del Riconoscimento Molecolare, 20131 Milano, Italy
    Anal Biochem 418:164-6. 2011
    ..Moreover, limits of detection of 45 and 54 pg/ml were obtained for hepatitis B superficial antigen and HIV p24 antigen, respectively...
  9. ncbi request reprint Protein and peptide arrays: recent trends and new directions
    Marina Cretich
    Istituto di Chimica del Riconoscimento Molecolare ICRM C N R, Via Mario Bianco, 9, 20131 Milano, Italy
    Biomol Eng 23:77-88. 2006
    ..This review article will focus on protein and peptide arrays highlighting their technical challenges and presenting new directions by means of a set of selected recent applications...
  10. doi request reprint Peptide microarrays on coated silicon slides for highly sensitive antibody detection
    Marina Cretich
    Istituto di Chimica del Riconoscimento Molecolare C N R, Milano, Italy
    Methods Mol Biol 669:147-60. 2010
    ..5-1 ng/mL in serum. The sensitivity provided by the test allows its use for the diagnosis of antibodies in clinical samples...
  11. doi request reprint High sensitivity protein assays on microarray silicon slides
    Marina Cretich
    Istituto di Chimica del Riconoscimento Molecolare, CNR, 20131 Milano, Italy
    Anal Chem 81:5197-203. 2009
    ..The coated silicon slides, tested in protein and peptide microarrays for detection of specific antibodies, lead to a 5-10-fold enhancement of the fluorescence signals in comparison to glass slides...
  12. doi request reprint Surface modifications by polymers for biomolecule conjugation
    Laura Sola
    Consiglio Nazionale delle Ricerche, Istituto di Chimica del Riconoscimento Molecolare, Milan, Italy
    Methods Mol Biol 1025:95-107. 2013
    ....
  13. doi request reprint Development of a high-sensitivity immunoassay for amyloid-beta 1-42 using a silicon microarray platform
    Paola Gagni
    Consiglio Nazionale delle Ricerche, Istituto di Chimica del Riconoscimento Molecolare ICRM, Via Mario Bianco, 9 20131 Milano, Italy
    Biosens Bioelectron 47:490-5. 2013
    ....
  14. doi request reprint Surface immobilized hydrogels as versatile reagent reservoirs for microarrays
    Laura Sola
    Consiglio Nazionale delle Ricerche, Istituto di Chimica del Riconoscimento Molecolare ICRM CNR, Milano, Italy
    J Immunol Methods 391:95-102. 2013
    ..Moreover, they prevent cross-reactivity issues in the detection of Staphylococcus aureus enterotoxins A and B and promote the accurate quantification of interleukin 10 by a microarray-integrated calibration curve...
  15. doi request reprint Interferometric silicon biochips for label and label-free DNA and protein microarrays
    Marina Cretich
    Consiglio Nazionale delle Ricerche, Istituto di Chimica del Riconoscimento Molecolare, Milano, Italy
    Proteomics 12:2963-77. 2012
    ..Finally, the importance of calibration of protein microarrays through the dual use of labeled and label-free detection schemes on the same chip will be illustrated...
  16. doi request reprint Epitope mapping of human chromogranin A by peptide microarrays
    Marina Cretich
    Istituto di Chimica del Riconoscimento Molecolare ICRM C N R Milano, Milano, Italy
    Methods Mol Biol 570:221-32. 2009
    ..The results reported are in accordance with those obtained in conventional ELISA assays using biotinylated and non-biotinylated peptides...
  17. ncbi request reprint Decreased protein peak asymmetry and width due to static capillary coating with hydrophilic derivatives of polydimethylacrylamide
    Marina Cretich
    Institute of Molecular Recognition Chemistry, CNR, Milano, Italy
    Electrophoresis 23:2274-8. 2002
    ..Coating agents which were more highly hydrophilic than those two (Table 1, No. 49) or less hydrophilic than 2% EPDMA (Table 1, Nos. 57, 53, 46) provided no stable static coating...
  18. ncbi request reprint Electroosmotic flow suppression in capillary electrophoresis: chemisorption of trimethoxy silane-modified polydimethylacrylamide
    Marina Cretich
    Istituto di Chimica del Riconoscimento Molecolare, CNR, I 20131 Milano, Italy
    Electrophoresis 26:1913-9. 2005
    ..In this research, we establish that physisorption of polymer chains to the surface is essential for close alignment of surface and polymer silane groups which facilitates the formation of covalent bonds...
  19. ncbi request reprint Screening Complex Biological Samples with Peptide Microarrays: The Favorable Impact of Probe Orientation via Chemoselective Immobilization Strategies on Clickable Polymeric Coatings
    Alessandro Gori
    Consiglio Nazionale delle Ricerche, Istituto di Chimica del Riconoscimento Molecolare ICRM Via Mario Bianco, 9, 20131 Milano, Italy
    Bioconjug Chem 27:2669-2677. 2016
    ..On the basis of our results, we critically discuss the immobilization parameters that need to be carefully considered for peptide ligand immobilization purposes...
  20. pmc Evolving serodiagnostics by rationally designed peptide arrays: the Burkholderia paradigm in Cystic Fibrosis
    Claudio Peri
    Istituto di Chimica del Riconoscimento Molecolare, ICRM, CNR Via Mario Bianco 9, 20131, Milano Italy
    Sci Rep 6:32873. 2016
    ..The method is general and immediately applicable to other bacteria. ..
  21. pmc Silicon biochips for dual label-free and fluorescence detection: Application to protein microarray development
    Marina Cretich
    Consiglio Nazionale delle Ricerche, Istituto di Chimica del Riconoscimento Molecolare ICRM, Milano, Italy
    Biosens Bioelectron 26:3938-43. 2011
    ....
  22. ncbi request reprint Nonconventional synthesis and characterization of ultrahigh-molar-mass polyacrylamides
    Marina Cretich
    Istituto di Chimica del Riconoscimento Molecolare, C N R, Milano, Italy
    Electrophoresis 24:2322-7. 2003
    ..We demonstrate that the sieving performance of polyacrylamide is directly correlated to its intrinsic viscosity...
  23. ncbi request reprint Microchips and single-photon avalanche diodes for DNA separation with high sensitivity
    Ivan Rech
    Dipartimento di Elettronica e Informazione, Politecnico di Milano, Milano, Italy
    Electrophoresis 27:3797-804. 2006
    ..The experimental results obtained in the separation of Cy5-labeled oligonucleotide demonstrate sensitivity better than 3 pM, which corresponds to less than 100 fluorescent molecules in the 50 pL illuminated volume...
  24. ncbi request reprint Peptide microarrays for the characterization of antigenic regions of human chromogranin A
    Marcella Chiari
    Istituto di Chimica del Riconoscimento Molecolare, Milan, Italy
    Proteomics 5:3600-3. 2005
    ..The results reported in this article are in accordance with those obtained in conventional ELISA assays using biotinylated and non-biotinylated peptides...
  25. doi request reprint Digital detection of biomarkers assisted by nanoparticles: application to diagnostics
    Marina Cretich
    Consiglio Nazionale delle Ricerche, Istituto di Chimica del Riconoscimento Molecolare ICRM, Milano, Italy
    Trends Biotechnol 33:343-51. 2015
    ..The discussion of the methods emphasizes the practical considerations and their clinical applicability. ..
  26. doi request reprint Novel fluorescent microarray platforms: a case study in neurodegenerative disorders
    Marina Cretich
    Consiglio Nazionale delle Ricerche, Istituto di Chimica del Riconoscimento Molecolare ICRM, Milano, Italy
    Expert Rev Mol Diagn 13:863-73. 2013
    ....
  27. doi request reprint Advanced polymers for molecular recognition and sensing at the interface
    Marcella Chiari
    Istituto di Chimica del Riconoscimento Molecolare C N R Via Mario Bianco, 9, 20131 Milano, Italy
    J Chromatogr B Analyt Technol Biomed Life Sci 866:89-103. 2008
    ..This review will provide very recent examples of polymer-mediated molecular recognition between guest molecules in solution and host molecules located at the solid phase...
  28. ncbi request reprint Clickable Polymeric Coating for Glycan Microarrays
    Caterina Zilio
    Istituto di Chimica del Riconoscimento Molecolare, CNR, Via Mario Bianco 9, 20131, Milan, Italy
    Methods Mol Biol 1518:55-65. 2017
    ..The proposed approach enables the attachment of complex sugars on a silicon oxide surface by a method that does not require skilled personnel and chemistry laboratories...
  29. doi request reprint Clickable Polymeric Coating for Oriented Peptide Immobilization
    Laura Sola
    Istituto di Chimica del Riconoscimento Molecolare ICRM, Consiglio Nazionale delle Ricerche CNR, Via Mario Bianco 9, Milan, 20131, Italy
    Methods Mol Biol 1352:167-82. 2016
    ..A similar coating that does not contain the alkyne functionality has been used as comparison, to demonstrate the importance of a proper orientation for facilitating the probe recognition and interaction with the target antibody. ..
  30. doi request reprint Rational epitope design for protein targeting
    Claudio Peri
    Isituto di Chimica del Riconoscimento Molecolare, CNR, Via Mario Bianco 9, 20131 Milano, Italy
    ACS Chem Biol 8:397-404. 2013
    ..We discuss implications for diagnosis and vaccine development purposes...
  31. ncbi request reprint Separation of proteins in a multicompartment electrolyzer with chambers defined by a bed of gel beads
    Marina Cretich
    Istituto di Chimica del Riconoscimento Molecolare, C N R, Milano, Italy
    Electrophoresis 24:577-81. 2003
    ..In order to overcome this problem, we propose to substitute the continuous membranes with a bed of gel beads of identical comonomer composition, obtained by an inverse emulsion polymerization process...
  32. ncbi request reprint Separation of DNA fragments in hydroxylated poly(dimethylacrylamide) copolymers
    Marcella Chiari
    Institute of Biocatalysis and Molecular Recognition, CNR, Milano, Italy
    Electrophoresis 23:536-41. 2002
    ..The new copolymers, due to their low viscosity high sieving capacity and ability to suppress EOF, represent a better alternative to PDMA and are suitable replaceable matrices for capillary and microchip electrophoresis...
  33. ncbi request reprint High-throughput mutational screening for beta-thalassemia by single-nucleotide extension
    Silvia Galbiati
    Genomic Unit for the Diagnosis of Human Pathologies, San Raffaele Scientific Institute, Milano, Italy
    Electrophoresis 28:4289-94. 2007
    ..The multicolour detection and the use of multiple injections further enhances the throughput of mutational screening by the DNA sequencer and facilitates automated genotyping for routine molecular diagnostics...
  34. doi request reprint Poly(N,N-Dimethylacrylamide)-Based Coatings to Modulate Electroosmotic Flow and Capillary Surface Properties for Protein Analysis
    Laura Sola
    Istituto di Chimica del Riconoscimento Molecolare, CNR, Via Mario Bianco 9, Milan, 20131, Italy
    Methods Mol Biol 1466:107-19. 2016
    ..Furthermore, the presence of these monomers makes it possible to modulate the electroosmotic flow from negligible to reduced values, depending on the desired application. ..
  35. doi request reprint Controlling electroosmotic flows by polymer coatings: A joint experimental-theoretical investigation
    Michele Monteferrante
    Consiglio Nazionale delle Ricerche, Istituto di Chimica del Riconoscimento Molecolare ICRM CNR, Via Mario Bianco, 20131 Milan, Italy
    J Chem Phys 143:184907. 2015
    ..A proposed application of the approach is to determine the near-wall charge of the coating to be used for further quantitative analysis of the electroosmotic flow and mobility. ..
  36. doi request reprint Combined mass quantitation and phenotyping of intact extracellular vesicles by a microarray platform
    Paola Gagni
    Consiglio Nazionale delle Ricerche, Istituto di Chimica del Riconoscimento Molecolare ICRM, Italy
    Anal Chim Acta 902:160-7. 2016
    ..The proposed method simultaneously detects, quantify and phenotype intact EVs in a microarray format. ..
  37. ncbi request reprint Acrylamide-agarose copolymers: improved resolution of high molecular mass proteins in two-dimensional gel electrophoresis
    Paola Roncada
    Istituto Sperimentale Italiano, L Spallanzani, Milan, Italy
    Proteomics 5:2331-9. 2005
    ..The results demonstrate that the new gel is fully compatible with mass spectrometry protein analysis...