Emilia Bramanti

Summary

Affiliation: CNR Institute of Clinical Physiology
Country: Italy

Publications

  1. ncbi Separation and determination of denatured caseins by hydrophobic interaction chromatography Part II. Method validation and applications
    E Bramanti
    National Council of Research, CNR, Institute of Instrumental Analytical Chemistry, Pisa, Italy
    Analyst 126:995-1000. 2001
  2. ncbi New chromatographic method for separation and determination of denatured alphaS1-, alphaS2-, beta- and kappa-caseins by hydrophobic interaction chromatography
    Emilia Bramanti
    National Council of Research, CNR, Istituto per i Processi Chimico Fisici, Pisa, Italy
    J Chromatogr A 958:157-66. 2002
  3. ncbi Separation and determination of denatured alpha(s1)-, alpha(s2)-, beta- and kappa-caseins by hydrophobic interaction chromatography in cows', ewes' and goats' milk, milk mixtures and cheeses
    Emilia Bramanti
    Italian National Research Council, CNR, Istituto per i Processi Chimico Fisici, Laboratory of Instrumental Analytical Chemistry, Via G Moruzzi 1, 56124 Pisa, Italy
    J Chromatogr A 994:59-74. 2003
  4. doi A kinetic study of gamma-glutamyltransferase (GGT)-mediated S-nitrosoglutathione catabolism
    Valeria Angeli
    National Research Council CNR, Inst for Chemico Physical Processes, Laboratory of Instrumental Analytical Chemistry, Via G Moruzzi 1 56124 Pisa, Italy
    Arch Biochem Biophys 481:191-6. 2009
  5. doi Determination of S-nitrosoglutathione in plasma: comparison of two methods
    Emilia Bramanti
    Italian National Research Council Istituto per i Processi Chimico Fisici, Laboratory of Instrumental Analytical Chemistry, Via G Moruzzi 1, Pisa, Italy
    Talanta 81:1295-9. 2010
  6. doi Exogenous vs. endogenous gamma-glutamyltransferase activity: Implications for the specific determination of S-nitrosoglutathione in biological samples
    Emilia Bramanti
    Italian National Research Council Istituto per i Processi Chimico Fisici, Laboratory of Instrumental and Analytical Chemistry, 56124 Pisa, Italy
    Arch Biochem Biophys 487:146-52. 2009
  7. doi Microwave photochemical reactor for the online oxidative decomposition of p-hydroxymercurybenzoate (pHMB)-tagged proteins and their determination by cold vapor generation-atomic fluorescence detection
    Beatrice Campanella
    National Research Council of Italy, C N R, Istituto di Chimica dei Composti Organo Metallici ICCOM UOS Pisa, Area di Ricerca, Via G Moruzzi 1, 56124 Pisa, Italy
    Anal Chem 85:12152-7. 2013
  8. ncbi Flow injection analysis with diode array absorbance detection and dynamic surface tension detection for studying denaturation and surface activity of globular proteins
    Emilia Bramanti
    CNR Institute for Chemical and Physical Processes IPCF, Laboratory of Instrumental Analytical Chemistry, 56124 Pisa, Italy
    Anal Biochem 351:100-13. 2006
  9. ncbi Characterization of denatured metallothioneins by reversed phase coupled with on-line chemical vapour generation and atomic fluorescence spectrometric detection
    Emilia Bramanti
    Laboratory of Instrumental Analytical Chemistry, Italian National Research Council Istituto per i Processi Chimico Fisici, Via G Moruzzi 1, 56124 Pisa, Italy
    J Chromatogr A 1054:285-91. 2004
  10. doi Characterization of BSA unfolding and aggregation using a single-capillary viscometer and dynamic surface tension detector
    Emilia Bramanti
    National Research Council of Italy, C N R, Istituto di Chimica dei Composti Organo Metallici ICCOM UOS Pisa, Area di Ricerca, Via G Moruzzi 1, 56124 Pisa, Italy
    Talanta 85:2553-61. 2011

Collaborators

Detail Information

Publications34

  1. ncbi Separation and determination of denatured caseins by hydrophobic interaction chromatography Part II. Method validation and applications
    E Bramanti
    National Council of Research, CNR, Institute of Instrumental Analytical Chemistry, Pisa, Italy
    Analyst 126:995-1000. 2001
    ....
  2. ncbi New chromatographic method for separation and determination of denatured alphaS1-, alphaS2-, beta- and kappa-caseins by hydrophobic interaction chromatography
    Emilia Bramanti
    National Council of Research, CNR, Istituto per i Processi Chimico Fisici, Pisa, Italy
    J Chromatogr A 958:157-66. 2002
    ....
  3. ncbi Separation and determination of denatured alpha(s1)-, alpha(s2)-, beta- and kappa-caseins by hydrophobic interaction chromatography in cows', ewes' and goats' milk, milk mixtures and cheeses
    Emilia Bramanti
    Italian National Research Council, CNR, Istituto per i Processi Chimico Fisici, Laboratory of Instrumental Analytical Chemistry, Via G Moruzzi 1, 56124 Pisa, Italy
    J Chromatogr A 994:59-74. 2003
    ....
  4. doi A kinetic study of gamma-glutamyltransferase (GGT)-mediated S-nitrosoglutathione catabolism
    Valeria Angeli
    National Research Council CNR, Inst for Chemico Physical Processes, Laboratory of Instrumental Analytical Chemistry, Via G Moruzzi 1 56124 Pisa, Italy
    Arch Biochem Biophys 481:191-6. 2009
    ..A K(m) of GGT for GSNO of 0.398+/-31 mM was thus found, comparable with K(m) values reported for other gamma-glutamyl substrates of GGT...
  5. doi Determination of S-nitrosoglutathione in plasma: comparison of two methods
    Emilia Bramanti
    Italian National Research Council Istituto per i Processi Chimico Fisici, Laboratory of Instrumental Analytical Chemistry, Via G Moruzzi 1, Pisa, Italy
    Talanta 81:1295-9. 2010
    ..Sampling issues connected with common blood sampling procedures like venipuncture and sampling in syringe or Vacutainers still introduce in GSNO analysis unknown factors, which require further investigations...
  6. doi Exogenous vs. endogenous gamma-glutamyltransferase activity: Implications for the specific determination of S-nitrosoglutathione in biological samples
    Emilia Bramanti
    Italian National Research Council Istituto per i Processi Chimico Fisici, Laboratory of Instrumental and Analytical Chemistry, 56124 Pisa, Italy
    Arch Biochem Biophys 487:146-52. 2009
    ....
  7. doi Microwave photochemical reactor for the online oxidative decomposition of p-hydroxymercurybenzoate (pHMB)-tagged proteins and their determination by cold vapor generation-atomic fluorescence detection
    Beatrice Campanella
    National Research Council of Italy, C N R, Istituto di Chimica dei Composti Organo Metallici ICCOM UOS Pisa, Area di Ricerca, Via G Moruzzi 1, 56124 Pisa, Italy
    Anal Chem 85:12152-7. 2013
    ..This method has been applied to the determination of thiolic peptides after tryptic digestion of serum albumins from different species (human, bovine, rat, horse, and sheep). ..
  8. ncbi Flow injection analysis with diode array absorbance detection and dynamic surface tension detection for studying denaturation and surface activity of globular proteins
    Emilia Bramanti
    CNR Institute for Chemical and Physical Processes IPCF, Laboratory of Instrumental Analytical Chemistry, 56124 Pisa, Italy
    Anal Biochem 351:100-13. 2006
    ....
  9. ncbi Characterization of denatured metallothioneins by reversed phase coupled with on-line chemical vapour generation and atomic fluorescence spectrometric detection
    Emilia Bramanti
    Laboratory of Instrumental Analytical Chemistry, Italian National Research Council Istituto per i Processi Chimico Fisici, Via G Moruzzi 1, 56124 Pisa, Italy
    J Chromatogr A 1054:285-91. 2004
    ..3 x 10(-9) mol l(-1) and for the denatured MTs LODc was about 8.6 x 10(-10) mol l(-1), taking into account an approximate complexating ratio PHMB:MTs of 7:1...
  10. doi Characterization of BSA unfolding and aggregation using a single-capillary viscometer and dynamic surface tension detector
    Emilia Bramanti
    National Research Council of Italy, C N R, Istituto di Chimica dei Composti Organo Metallici ICCOM UOS Pisa, Area di Ricerca, Via G Moruzzi 1, 56124 Pisa, Italy
    Talanta 85:2553-61. 2011
    ..The results have been supported and discussed with respect to BSA conformational analysis performed using Fourier Transform infrared spectroscopy...
  11. ncbi Study of the disulfide reduction of denatured proteins by liquid chromatography coupled with on-line cold-vapor-generation atomic-fluorescence spectrometry (LC-CVGAFS)
    Emilia Bramanti
    Laboratory of Instrumental Analytical Chemistry, Italian National Research Council Istituto per i Processi Chimico Fisici, Via G Moruzzi 1, 56124 Pisa, Italy
    Anal Bioanal Chem 380:310-8. 2004
    ..The detection limit for denatured, reduced proteins examined under the optimized conditions was found to be in the range 1-5 x 10(-12) mol L(-1) (10-30 pg), depending on the protein considered...
  12. doi Derivatization of GSSG by pHMB in alkaline media. Determination of oxidized glutathione in blood
    Valeria Angeli
    Italian National Research Council Istituto per i Processi Chimico Fisici, Laboratory of Instrumental Analytical Chemistry, Pisa, Italy
    Talanta 82:815-20. 2010
    ..The proposed method was applied to the determination of GSSG in whole blood and validated by the classical determination of GSSG by derivatization after reduction with dithiothreitol (DTT)...
  13. ncbi Determination and characterization of phytochelatins by liquid chromatography coupled with on line chemical vapour generation and atomic fluorescence spectrometric detection
    Emilia Bramanti
    Italian National Research Council, CNR Istituto per i Processi Chimico Fisici, Laboratory of Instrumental Analytical Chemistry, Via G Moruzzi 1, 56124 Pisa, Italy
    J Chromatogr A 1133:195-203. 2006
    ..2 pmol (0.97 ng) of GSH. The method has been applied to analysis of extracts of cell cultures from Phaeodactylum tricornutum grown in Cd-containing nutrient solutions, analysed by SEC-DAD-CVGAFS and RPLC-DAD-CVGAFS...
  14. doi Determination of thiolic compounds as mercury complexes by cold vapor atomic absorption spectrometry and its application to wines
    Emilia Bramanti
    Italian National Research Council CNR, Institute for Chemical and Physical Processes, IPCF Laboratory of Instrumental Analytical Chemistry, Via G Moruzzi 1, 56124 Pisa, Italy
    Talanta 74:936-43. 2008
    ..The method has been applied to quantification of thiol groups in wine samples. Comparison with results obtained by HPLC coupled to atomic fluorescence detection confirmed the promising potentialities of the method...
  15. ncbi Multidimensional analysis of denatured milk proteins by hydrophobic interaction chromatography coupled to a dynamic surface tension detector
    Emilia Bramanti
    Laboratory of Instrumental Analytical Chemistry, Istituto per i Processi Chimico Fisici, National Research Council of Italy CNR, Via G Moruzzi 1, 56124 Pisa, Italy
    J Chromatogr A 1023:79-91. 2004
    ..The different samples were readily distinguished based upon the selectivity provided by the HIC-DSTD method. The selectivity advantage of using DSTD relative to absorbance detection is also demonstrated...
  16. doi Direct, simple derivatization of disulfide bonds in proteins with organic mercury in alkaline medium without any chemical pre-reducing agents
    Beatrice Campanella
    National Research Council of Italy, C N R, Istituto di Chimica dei Composti Organo Metallici ICCOM UOS Pisa, Area di Ricerca, Via G Moruzzi 1, 56124 Pisa, Italy
    Anal Chim Acta 843:1-6. 2014
    ....
  17. doi Determination of thiocyanate in saliva by headspace gas chromatography-mass spectrometry, following a single-step aqueous derivatization with triethyloxonium tetrafluoroborate
    Sara Ammazzini
    C N R, Institute of Chemistry of OrganoMetallic Compounds, UOS of Pisa, Via Moruzzi, 1, 56124 Pisa, Italy University of Pisa, Department of Chemistry and Industrial Chemistry, Via Risorgimento, 35, 56125 Pisa, Italy
    J Chromatogr A 1400:124-30. 2015
    ..This method was applied for the determination of thiocyanate in human saliva samples...
  18. ncbi Speciation and quantification of thiols by reversed-phase chromatography coupled with on-line chemical vapor generation and atomic fluorescence spectrometric detection: method validation and preliminary application for glutathione measurements in human wh
    Emilia Bramanti
    Italian National Research Council, Istituto per i Processi Chimico Fisici, Laboratory of Instrumental Analytical Chemistry, Pisa, Italy
    Clin Chem 51:1007-13. 2005
    ..We developed a sensitive, specific method for the low-molecular-mass thiols cysteine, cysteinylglycine, glutathione, and homocysteine and validated the method for measurement of glutathione in blood...
  19. ncbi Characterization of denatured proteins by hydrophobic interaction chromatography: a preliminary study
    Emilia Bramanti
    National Council of Research CNR, Istituto per i Processi Chimico Fisici, Laboratory of Instrumental Analytical Chemistry, Via G Moruzzi 1, 56124 Pisa, Italy
    Biopolymers 69:293-300. 2003
    ..Suitable elution conditions provide evidence of the simultaneous presence of two denatured forms in the case of MYO, and sequential different denatured states of Cyt C...
  20. doi Ovalbumin labeling with p-hydroxymercurybenzoate: The effect of different denaturing agents and the kinetics of reaction
    Beatrice Campanella
    Istituto di Chimica dei Composti Organo Metallici ICCOM National Research Council of Italy CNR, UoS di Pisa, 56124 Pisa, Italy Dipartimento di Chimica e Chimica Industriale, Universita di Pisa, 56124 Pisa, Italy
    Anal Biochem 483:27-33. 2015
    ..We also investigated the labeling kinetics for denatured OVA and pHMB, finding that the 4 thiolic groups of OVA have a very different reactivity toward mercury labeling, in agreement with previous studies...
  21. doi Human exposure to thallium through tap water: A study from Valdicastello Carducci and Pietrasanta (northern Tuscany, Italy)
    Beatrice Campanella
    C N R Istituto di Chimica dei Composti Organometallici, UoS di Pisa, Via Moruzzi 1, 56124 Pisa, Italy Università di Pisa, Dipartimento di Chimica e Chimica Industriale, Via Moruzzi 13, 56124 Pisa, Italy
    Sci Total Environ 548:33-42. 2016
    ..The kinetics of decay of Tl concentration in urine samples was also investigated. At the best of our knowledge, this is the first study on human contamination by Tl through water involving such a high number of samples. ..
  22. doi Impact of protein concentration on the determination of thiolic groups of ovalbumin: a size exclusion chromatography-chemical vapor generation-atomic fluorescence spectrometry study via mercury labeling
    Beatrice Campanella
    National Research Council of Italy, Istituto di Chimica dei Composti Organo Metallici UOS Pisa, Area di Ricerca, Via G Moruzzi 1, 56124 Pisa, Italy
    Anal Chem 86:2251-6. 2014
    ....
  23. doi Interaction of collagen with chlorosulphonated paraffin tanning agents: Fourier transform infrared spectroscopic analysis and molecular dynamics simulations
    Susanna Monti
    CNR Institute of Chemistry of Organometallic Compounds UOS di Pisa, Area della Ricerca, Via G Moruzzi 1, I 56124 Pisa, Italy
    Phys Chem Chem Phys 15:14736-47. 2013
    ....
  24. doi Determination of thiomersal by flow injection coupled with microwave-assisted photochemical online oxidative decomposition of organic mercury and cold vapor atomic fluorescence spectroscopy
    Beatrice Campanella
    National Research Council of Italy, C N R, Istituto di Chimica dei Composti Organo Metallici ICCOM UOS Pisa, Area di Ricerca, Via G Moruzzi 1, 56124 Pisa, Italy
    Anal Chim Acta 804:66-9. 2013
    ..We found a mercury concentration in commercial ophthalmic solutions ranging between 7.5 and 59.0 μg mL(-1)...
  25. doi Physico-chemical characterization of protein-pigment interactions in tempera paint reconstructions: casein/cinnabar and albumin/cinnabar
    Celia Duce
    Dipartimento di Chimica e Chimica Industriale, Pisa, Italy
    Anal Bioanal Chem 402:2183-93. 2012
    ..The study clearly showed that HgS forms stable complexes with proteins and acts as a sensitizer in cross-linking, hydrolysis and oxidation...
  26. ncbi Microwave-Assisted Photochemical Reactor for the Online Oxidative Decomposition and Determination of p-Hydroxymercurybenzoate and Its Thiolic Complexes by Cold Vapor Generation Atomic Fluorescence Detection
    Valeria Angeli
    Consiglio Nazionale delle Ricerche, Istituto di Chimica dei Composti Organometallici, Area di Ricerca, Via G Moruzzi 1 56124 Pisa, Italy, and Italian National Research Council INO UOS PISA, Consiglio Nazionale delle Ricerche, Istituto Nazionale di Ottica, Area di Ricerca, Via G Moruzzi 1, 56124 Pisa, Italy
    Anal Chem 83:338-43. 2011
    ..This avoided peak widening due to diffusion processes generally occurring at high temperatures and in the additional cooling coil. This method has been applied to the determination of thiols in human plasma, blood, and wine...
  27. pmc Effects of sucrose on the internal dynamics of azurin
    Patrizia Cioni
    Istituto di Biofisica, Consiglio Nazionale delle Ricerche, Pisa, Italy
    Biophys J 88:4213-22. 2005
    ..Preliminary tests with trehalose and xylitol suggest that the effects of sucrose are general of the polyol class of osmolytes...
  28. ncbi A high performance gel filtration chromatography method for gamma-glutamyltransferase fraction analysis
    Maria Franzini
    Scuola Superiore S Anna, Pisa, Italy
    Anal Biochem 374:1-6. 2008
    ..Higher total GGT activity in males is related mainly to f-GGT (P<0.01). GGT fraction analysis may increase the sensitivity and specificity of the GGT activity test...
  29. doi Development and validation of a novel derivatization method for the determination of lactate in urine and saliva by liquid chromatography with UV and fluorescence detection
    Davide Pellegrini
    National Research Council of Italy, C N R, Istituto di Chimica dei Composti Organo Metallici ICCOM UOS Pisa, Area di Ricerca, Via G Moruzzi 1, 56124 Pisa, Italy
    Talanta 130:280-7. 2014
    ..Intra-day and inter-day repeatability were lower than 5% and 6%, respectively. The method proposed was successfully applied to the analysis of urine and saliva samples...
  30. doi Detection of proteins by hyphenated techniques with endogenous metal tags and metal chemical labelling
    Beatrice Campanella
    National Research Council of Italy, C N R, Istituto di Chimica dei Composti Organo Metallici ICCOM UOS Pisa, Area di Ricerca, Via G Moruzzi 1, 56124 Pisa, Italy
    Analyst 139:4124-53. 2014
    ....
  31. doi β-Amyloid amorphous aggregates induced by the small natural molecule ferulic acid
    Emilia Bramanti
    Istituto dei Composti Organo Metallici, Istituto di Biofisica, and Istituto Nazionale di Ottica, CNR, U O Pisa, Via G Moruzzi, 1, 56124, Pisa, Italy
    J Phys Chem B 117:13816-21. 2013
    ..These findings highlight the molecular basis underlying FA antiamyloidogenic activity in AD. ..
  32. doi Simultaneous determination of lactate and pyruvate in human sweat using reversed-phase high-performance liquid chromatography: a noninvasive approach
    Simona Biagi
    National Research Council of Italy, C N R, Istituto per i Processi Chimico Fisici IPCF UOS Pisa, Area di Ricerca, Via G Moruzzi 1, 56124, Pisa, Italy
    Biomed Chromatogr 26:1408-15. 2012
    ..1 and 96 ± 0.1 for lactate and pyruvate, respectively. The method was successfully applied to analysis of sweat in 8 athletes at rest (pilocarpine sweating) and during physical exercise...
  33. doi Fractions of plasma gamma-glutamyltransferase in healthy individuals: reference values
    Maria Franzini
    Clin Chim Acta 395:188-9. 2008
  34. ncbi Determination, by dynamic surface-tension analysis, of the molar mass of proteins denatured in guanidine thiocyanate
    Wes W C Quigley
    Center for Process Analytical Chemistry, Department of Chemistry, University of Washington, Box 351700, Seattle, WA 98195, USA
    Anal Bioanal Chem 378:134-43. 2004
    ....