Etana Padan

Summary

Affiliation: The Hebrew University
Country: Israel

Publications

  1. pmc Alkaline pH homeostasis in bacteria: new insights
    Etana Padan
    Alexander Silberman Institute of Life Sciences, Hebrew University, Jerusalem 91904, Israel
    Biochim Biophys Acta 1717:67-88. 2005
  2. doi request reprint Sodium-Proton (Na(+)/H(+)) Antiporters: Properties and Roles in Health and Disease
    Etana Padan
    Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, 91904, Jerusalem, Israel
    Met Ions Life Sci 16:391-458. 2016
  3. pmc NhaA antiporter functions using 10 helices, and an additional 2 contribute to assembly/stability
    Etana Padan
    Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, Hebrew University, 91904 Jerusalem, Israel
    Proc Natl Acad Sci U S A 112:E5575-82. 2015
  4. doi request reprint Overexpression, Isolation, Purification, and Crystallization of NhaA
    Etana Padan
    Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem, Israel Electronic address
    Methods Enzymol 557:135-48. 2015
  5. doi request reprint Regulation of NhaA by protons
    Etana Padan
    Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel
    Compr Physiol 1:1711-9. 2011
  6. doi request reprint The enlightening encounter between structure and function in the NhaA Na+-H+ antiporter
    Etana Padan
    Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    Trends Biochem Sci 33:435-43. 2008
  7. ncbi request reprint Na(+)/H(+) antiporters
    E Padan
    Department of Microbial and Molecular Ecology, Institute of Life Sciences, Hebrew University of Jerusalem, Israel
    Biochim Biophys Acta 1505:144-57. 2001
  8. ncbi request reprint NhaA of Escherichia coli, as a model of a pH-regulated Na+/H+antiporter
    E Padan
    Division of Microbial and Molecular Ecology, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    Biochim Biophys Acta 1658:2-13. 2004
  9. doi request reprint NhaA crystal structure: functional-structural insights
    Etana Padan
    Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    J Exp Biol 212:1593-603. 2009
  10. ncbi request reprint Mutation E252C increases drastically the Km value for Na+ and causes an alkaline shift of the pH dependence of NhaA Na+/H+ antiporter of Escherichia coli
    Tzvi Tzubery
    Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    J Biol Chem 279:3265-72. 2004

Collaborators

Detail Information

Publications35

  1. pmc Alkaline pH homeostasis in bacteria: new insights
    Etana Padan
    Alexander Silberman Institute of Life Sciences, Hebrew University, Jerusalem 91904, Israel
    Biochim Biophys Acta 1717:67-88. 2005
    ..This review highlights the approaches, major findings and unresolved problems in alkaline pH homeostasis, focusing on the small number of well-characterized alkali-tolerant and extremely alkaliphilic bacteria...
  2. doi request reprint Sodium-Proton (Na(+)/H(+)) Antiporters: Properties and Roles in Health and Disease
    Etana Padan
    Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, 91904, Jerusalem, Israel
    Met Ions Life Sci 16:391-458. 2016
    ....
  3. pmc NhaA antiporter functions using 10 helices, and an additional 2 contribute to assembly/stability
    Etana Padan
    Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, Hebrew University, 91904 Jerusalem, Israel
    Proc Natl Acad Sci U S A 112:E5575-82. 2015
    ..The findings demonstrate that the truncated mutants retain significant activity and regulatory properties but are defective in the assembly/stability of the Ec-NhaA dimer. ..
  4. doi request reprint Overexpression, Isolation, Purification, and Crystallization of NhaA
    Etana Padan
    Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem, Israel Electronic address
    Methods Enzymol 557:135-48. 2015
    ..5 and reaches maximal activity at pH8.5. Therefore, it is crucial to crystallize the active conformations of NhaA. Herein, we present a procedure for determining the structure of NhaA. ..
  5. doi request reprint Regulation of NhaA by protons
    Etana Padan
    Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel
    Compr Physiol 1:1711-9. 2011
    ..Thus, integrating structural, computational, and experimental approaches are essential to reveal how the pH signal is perceived and transduced to activate the pH regulated protein...
  6. doi request reprint The enlightening encounter between structure and function in the NhaA Na+-H+ antiporter
    Etana Padan
    Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    Trends Biochem Sci 33:435-43. 2008
    ..This novel structural fold creates a delicately balanced electrostatic environment in the middle of the membrane, which might be essential for ion binding and translocation...
  7. ncbi request reprint Na(+)/H(+) antiporters
    E Padan
    Department of Microbial and Molecular Ecology, Institute of Life Sciences, Hebrew University of Jerusalem, Israel
    Biochim Biophys Acta 1505:144-57. 2001
    ..It is envisaged that interdisciplinary approaches combining structure, molecular and cell biology as well as genomics should be applied in the future to the study of this important group of transporters...
  8. ncbi request reprint NhaA of Escherichia coli, as a model of a pH-regulated Na+/H+antiporter
    E Padan
    Division of Microbial and Molecular Ecology, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    Biochim Biophys Acta 1658:2-13. 2004
    ..Test of this possibility awaits the atomic resolution of the 3D structure of the antiporters...
  9. doi request reprint NhaA crystal structure: functional-structural insights
    Etana Padan
    Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    J Exp Biol 212:1593-603. 2009
    ..Based on the crystal structure of NhaA, a model structure of the human Na(+)/H(+) exchanger (NHE1) was constructed, paving the way to a rational drug design...
  10. ncbi request reprint Mutation E252C increases drastically the Km value for Na+ and causes an alkaline shift of the pH dependence of NhaA Na+/H+ antiporter of Escherichia coli
    Tzvi Tzubery
    Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    J Biol Chem 279:3265-72. 2004
    ....
  11. pmc Transmembrane segment II of NhaA Na+/H+ antiporter lines the cation passage, and Asp65 is critical for pH activation of the antiporter
    Katia Herz
    Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, Hebrew University, 91904 Jerusalem, Israel
    J Biol Chem 285:2211-20. 2010
    ..5 (10-fold) but not at pH 8.5, implying the importance of Asp(65) negative charge for pH activation of the antiporter...
  12. pmc Revealing the ligand binding site of NhaA Na+/H+ antiporter and its pH dependence
    Michal Maes
    Institute of Chemistry, Alexander Silberman Institute of Life Sciences, Faculty of Sciences, Hebrew University of Jerusalem, Safra Campus, Givat Ram, Jerusalem 91904, Israel
    J Biol Chem 287:38150-7. 2012
    ....
  13. ncbi request reprint Functional and structural interactions of the transmembrane domain X of NhaA, Na+/H+ antiporter of Escherichia coli, at physiological pH
    Lena Kozachkov
    Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem 91904, Israel
    Biochemistry 46:2419-30. 2007
    ..These results reveal that, at alkaline pH, the distance between the conserved center of helix X and E78 of helix II is drastically decreased, implying a pH-induced conformational change of one or both helices...
  14. doi request reprint Helix VIII of NhaA Na(+)/H(+) antiporter participates in the periplasmic cation passage and pH regulation of the antiporter
    Mohammad Diab
    Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, Hebrew University, 91904 Jerusalem, Israel
    J Mol Biol 413:604-14. 2011
    ..Taken together, our results at physiological pH reveal that amino acid residues in transmembrane VIII contribute to the cation passage of NhaA and its pH regulation...
  15. ncbi request reprint Unraveling functional and structural interactions between transmembrane domains IV and XI of NhaA Na+/H+ antiporter of Escherichia coli
    Livnat Galili
    Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem 91904, Israel
    J Biol Chem 279:23104-13. 2004
    ..Hence, cross-linking at this position restores an active conformation of NhaA...
  16. pmc Structure-based functional study reveals multiple roles of transmembrane segment IX and loop VIII-IX in NhaA Na+/H+ antiporter of Escherichia coli at physiological pH
    Tzvi Tzubery
    Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    J Biol Chem 283:15975-87. 2008
    ....
  17. doi request reprint Beta-sheet-dependent dimerization is essential for the stability of NhaA Na+/H+ antiporter
    Katia Herz
    Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    J Biol Chem 284:6337-47. 2009
    ..We suggest that NhaA dimers are crucial for the stability of the antiporter under extreme stress conditions...
  18. pmc Site-directed tryptophan fluorescence reveals two essential conformational changes in the Na+/H+ antiporter NhaA
    Lena Kozachkov
    Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem 91904, Israel
    Proc Natl Acad Sci U S A 108:15769-74. 2011
    ....
  19. pmc Alkalitolerance: a biological function for a multidrug transporter in pH homeostasis
    Oded Lewinson
    Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel
    Proc Natl Acad Sci U S A 101:14073-8. 2004
    ..coli from an obligatory neutrophile into an alkalitolerant bacterium, and they suggest a previously uncharacterized physiological role for MdfA in pH homeostasis...
  20. doi request reprint The unwound portion dividing helix IV of NhaA undergoes a conformational change at physiological pH and lines the cation passage
    Abraham Rimon
    Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, Hebrew University, 91904 Jerusalem, Israel
    Biochemistry 51:9560-9. 2012
    ..5 to 8.5 induces conformational changes in helix IVp and in the extended chain at physiological pH...
  21. ncbi request reprint Monomers of the NhaA Na+/H+ antiporter of Escherichia coli are fully functional yet dimers are beneficial under extreme stress conditions at alkaline pH in the presence of Na+ or Li+
    Abraham Rimon
    Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    J Biol Chem 282:26810-21. 2007
    ..Remarkably, under extreme stress conditions (0.1 m LiCl or 0.7 m NaCl at pH 8.5), the dimeric native NhaA was much more efficient than the monomeric mutant in conferring extreme stress resistance...
  22. doi request reprint Epitope mapping of conformational monoclonal antibodies specific to NhaA Na+/H+ antiporter: structural and functional implications
    Abraham Rimon
    Department of Biochemistry, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    J Mol Biol 379:471-81. 2008
    ..The revealed location of the mAbs suggests that mAb binding distorts the unique NhaA TMS IV/XI assembly and thus inhibits the activity of NhaA. The noninhibitory mAb 6F9 binds to the functionally dispensable C-terminus of NhaA...
  23. ncbi request reprint Trans membrane domain IV is involved in ion transport activity and pH regulation of the NhaA-Na(+)/H(+) antiporter of Escherichia coli
    Livnat Galili
    Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    Biochemistry 41:609-17. 2002
    ..Cys accessibility test demonstrated that although D133C is located in the middle of TMS IV, it is inhibited by N-ethylmaleimide and is exposed to the cytoplasm...
  24. pmc Roles of NhaA, NhaB, and NhaD Na+/H+ antiporters in survival of Vibrio cholerae in a saline environment
    Katia Herz
    Department of Microbial and Molecular Ecology, Institute of Life Sciences, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    J Bacteriol 185:1236-44. 2003
    ..Our results indicate that the Na+ resistance of the human pathogen V. cholerae requires a complex molecular system involving multiple antiporters and the NQR pump...
  25. doi request reprint Conformational changes in NhaA Na+/H+ antiporter
    Lena Kozachkov
    Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Israel
    Mol Membr Biol 30:90-100. 2013
    ..The results reveal two different conformational changes characterizing NhaA: One is pH-induced for NhaA activation; the other is ligand-induced for antiport activity...
  26. pmc The Ec-NhaA antiporter switches from antagonistic to synergistic antiport upon a single point mutation
    Manish Dwivedi
    Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, Givat Ram, Jerusalem 91904, Israel
    Sci Rep 6:23339. 2016
    ..Most striking, however, was the mutation, A167P, which converted the Ec-NhaA antagonistic binding into synergistic binding which is only known to occur in Cl(-)/H(+) antiporter. ..
  27. pmc NhaA Na+/H+ antiporter mutants that hardly react to the membrane potential
    Dudu Alkoby
    Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, Hebrew University, Jerusalem, Israel
    PLoS ONE 9:e93200. 2014
    ..Detailed kinetic analysis reveals that in both mutants the rate limiting step of the cation exchange cycle is changed from an electrogenic to an electroneutral reaction. ..
  28. ncbi request reprint Crucial steps in the structure determination of the Na+/H+ antiporter NhaA in its native conformation
    Emanuela Screpanti
    Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max von Laue Str 3, D 60438 Frankfurt, Germany
    J Mol Biol 362:192-202. 2006
    ..Thus, the antiporter is in a native conformation in the 3D crystals...
  29. pmc Multiconformation continuum electrostatics analysis of the NhaA Na+/H+ antiporter of Escherichia coli with functional implications
    Elena Olkhova
    Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max von Laue Strasse 3, D 60438 Frankfurt am Main, Germany
    Proc Natl Acad Sci U S A 103:2629-34. 2006
    ..The results of the calculations provide valuable information on the activation of the antiporter and the role of individual amino acid residues, and provide a solid framework for further experiments...
  30. ncbi request reprint Structure of a Na+/H+ antiporter and insights into mechanism of action and regulation by pH
    Carola Hunte
    Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max von Laue Str 3, D 60438 Frankfurt, Germany
    Nature 435:1197-202. 2005
    ..This ion-exchange machinery is regulated by a conformational change elicited by a pH signal perceived at the entry to the cytoplasmic funnel...
  31. pmc The influence of protonation states on the dynamics of the NhaA antiporter from Escherichia coli
    Elena Olkhova
    Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, D 60438 Frankfurt am Main, Germany
    Biophys J 92:3784-91. 2007
    ..In the variant Gly(338)Ser that lost pH control, a hydrogen-bonded chain between Ser(338) and Lys(300) was found to block the pH-induced conformational reorganization of helix X...
  32. pmc Assessing oligomerization of membrane proteins by four-pulse DEER: pH-dependent dimerization of NhaA Na+/H+ antiporter of E. coli
    Daniel Hilger
    Ludwig Maximilians Universitat Munchen, Department Biologie I, Germany
    Biophys J 89:1328-38. 2005
    ....
  33. ncbi request reprint 2-Aminoperimidine, a specific inhibitor of bacterial NhaA Na(+)/H(+) antiporters
    Pavel Dibrov
    Department of Microbiology, University of Manitoba, Winnipeg, Man, Canada R3T 2N2
    FEBS Lett 579:373-8. 2005
    ..The inhibitor is specific to NhaA type antiporters, so AP is a new tool to study the mechanism and roles of NhaA antiporters of enterobacteria as well as the molecular basis of inhibition by an amiloride-like compound...
  34. ncbi request reprint Model structure of the Na+/H+ exchanger 1 (NHE1): functional and clinical implications
    Meytal Landau
    Department of Biochemistry, George S Wise Faculty of Life Sciences, Tel Aviv University, 69978, Tel Aviv, Israel
    J Biol Chem 282:37854-63. 2007
    ..We also suggest a hypothetical alternating-access mechanism that involves conformational changes...
  35. ncbi request reprint Do physiological roles foster persistence of drug/multidrug-efflux transporters? A case study
    Terry A Krulwich
    Department of Pharmacology and Biological Chemistry, Mount Sinai School of Medicine, New York, New York 10029, USA
    Nat Rev Microbiol 3:566-72. 2005
    ..The recent observations that Tet(L), a tetracycline-efflux transporter, and MdfA, a multidrug-efflux transporter, both confer alkali tolerance offer a striking case study in support of this hypothesis...