B I Kanner

Summary

Affiliation: The Hebrew University
Country: Israel

Publications

  1. ncbi request reprint Proximity of two oppositely oriented reentrant loops in the glutamate transporter GLT-1 identified by paired cysteine mutagenesis
    Lihi Brocke
    Department of Biochemistry, Hadassah Medical School, Hebrew University, Jerusalem 91120, Israel
    J Biol Chem 277:3985-92. 2002
  2. pmc Aspartate-444 is essential for productive substrate interactions in a neuronal glutamate transporter
    Shlomit Teichman
    Department of Biochemistry, Hebrew University Hadassah Medical School, Jerusalem 91120, Israel
    J Gen Physiol 129:527-39. 2007
  3. ncbi request reprint Transmembrane domain I of the gamma-aminobutyric acid transporter GAT-1 plays a crucial role in the transition between cation leak and transport modes
    Baruch I Kanner
    Department of Biochemistry, Hadassah Medical School, Hebrew University, Jerusalem 91120, Israel
    J Biol Chem 278:3705-12. 2003
  4. ncbi request reprint Structure and function of sodium-coupled GABA and glutamate transporters
    Baruch I Kanner
    Dept of Biochemistry, Hebrew University, Hadassah Medical School, P O Box 12272, Jerusalem 91120, Israel
    J Membr Biol 213:89-100. 2006
  5. ncbi request reprint Gate movements in glutamate transporters
    Baruch I Kanner
    Department of Biochemistry, Hebrew University, Hadassah Medical School, P O Box 12272, Jerusalem 91120, Israel
    ACS Chem Biol 2:163-6. 2007
  6. doi request reprint Sodium-coupled neurotransmitter transporters
    Baruch I Kanner
    Department of Biochemistry, Hebrew University, Hadassah Medical School, Post Office Box 12272, Jerusalem 91120, Israel
    Chem Rev 108:1654-68. 2008
  7. ncbi request reprint Molecular characterization of substrate-binding sites in the glutamate transporter family
    B I Kanner
    Department of Biochemistry, Hadassah Medical School, The Hebrew University, P O Box 12272, Jerusalem 9110, Israel
    Biochem Soc Trans 29:707-10. 2001
  8. ncbi request reprint The dual-function glutamate transporters: structure and molecular characterisation of the substrate-binding sites
    B I Kanner
    Department of Biochemistry, Hadassah Medical School, The Hebrew University, Jerusalem, Israel
    Biochim Biophys Acta 1555:92-5. 2002
  9. ncbi request reprint Tyrosine 140 of the gamma-aminobutyric acid transporter GAT-1 plays a critical role in neurotransmitter recognition
    Y Bismuth
    Department of Biochemistry, Hadassah Medical School, The Hebrew University, Jerusalem 91120, Israel
    J Biol Chem 272:16096-102. 1997
  10. ncbi request reprint Coupled, but not uncoupled, fluxes in a neuronal glutamate transporter can be activated by lithium ions
    L Borre
    Department of Biochemistry, Hadassah Medical School, The Hebrew University, Jerusalem 91120, Israel
    J Biol Chem 276:40396-401. 2001

Collaborators

Detail Information

Publications34

  1. ncbi request reprint Proximity of two oppositely oriented reentrant loops in the glutamate transporter GLT-1 identified by paired cysteine mutagenesis
    Lihi Brocke
    Department of Biochemistry, Hadassah Medical School, Hebrew University, Jerusalem 91120, Israel
    J Biol Chem 277:3985-92. 2002
    ..Positions 364 and 440 are located on reentrant loop I and II, respectively. Our results suggest that these two loops, previously shown to be essential for glutamate transport, come in close proximity...
  2. pmc Aspartate-444 is essential for productive substrate interactions in a neuronal glutamate transporter
    Shlomit Teichman
    Department of Biochemistry, Hebrew University Hadassah Medical School, Jerusalem 91120, Israel
    J Gen Physiol 129:527-39. 2007
    ....
  3. ncbi request reprint Transmembrane domain I of the gamma-aminobutyric acid transporter GAT-1 plays a crucial role in the transition between cation leak and transport modes
    Baruch I Kanner
    Department of Biochemistry, Hadassah Medical School, Hebrew University, Jerusalem 91120, Israel
    J Biol Chem 278:3705-12. 2003
    ..Our observations suggest the possibility that the permeation pathway in both modes shares common structural elements...
  4. ncbi request reprint Structure and function of sodium-coupled GABA and glutamate transporters
    Baruch I Kanner
    Dept of Biochemistry, Hebrew University, Hadassah Medical School, P O Box 12272, Jerusalem 91120, Israel
    J Membr Biol 213:89-100. 2006
    ..In this review the functional results on prototypes of the two families, the GABA transporter GAT-1 and the glutamate transporters GLT-1 and EAAC1, are described and discussed within the perspective provided by the novel structures...
  5. ncbi request reprint Gate movements in glutamate transporters
    Baruch I Kanner
    Department of Biochemistry, Hebrew University, Hadassah Medical School, P O Box 12272, Jerusalem 91120, Israel
    ACS Chem Biol 2:163-6. 2007
    ..These structures provide important insights into the molecular mechanism of ion-coupled transporters...
  6. doi request reprint Sodium-coupled neurotransmitter transporters
    Baruch I Kanner
    Department of Biochemistry, Hebrew University, Hadassah Medical School, Post Office Box 12272, Jerusalem 91120, Israel
    Chem Rev 108:1654-68. 2008
  7. ncbi request reprint Molecular characterization of substrate-binding sites in the glutamate transporter family
    B I Kanner
    Department of Biochemistry, Hadassah Medical School, The Hebrew University, P O Box 12272, Jerusalem 9110, Israel
    Biochem Soc Trans 29:707-10. 2001
    ..In these structural elements, we have identified several conserved amino acid residues that play crucial roles in the interaction with the transporter substrates sodium, potassium and glutamate...
  8. ncbi request reprint The dual-function glutamate transporters: structure and molecular characterisation of the substrate-binding sites
    B I Kanner
    Department of Biochemistry, Hadassah Medical School, The Hebrew University, Jerusalem, Israel
    Biochim Biophys Acta 1555:92-5. 2002
    ..The conformation of the transporter gating the anion conductance is different from that during substrate translocation. However, there exists a dynamic equilibrium between these conformations...
  9. ncbi request reprint Tyrosine 140 of the gamma-aminobutyric acid transporter GAT-1 plays a critical role in neurotransmitter recognition
    Y Bismuth
    Department of Biochemistry, Hadassah Medical School, The Hebrew University, Jerusalem 91120, Israel
    J Biol Chem 272:16096-102. 1997
    ..The total conservation of the residue throughout the family suggests that tyrosine 140 may be involved in the liganding of the amino group, the moiety common to all of the neurotransmitters...
  10. ncbi request reprint Coupled, but not uncoupled, fluxes in a neuronal glutamate transporter can be activated by lithium ions
    L Borre
    Department of Biochemistry, Hadassah Medical School, The Hebrew University, Jerusalem 91120, Israel
    J Biol Chem 276:40396-401. 2001
    ..Our observations indicate that although both the coupled and the uncoupled fluxes are sodium-dependent, the conformation gating the anion conductance is different from that during substrate translocation...
  11. ncbi request reprint Histidine 326 is critical for the function of GLT-1, a (Na+ + K+)-coupled glutamate transporter from rat brain
    Y Zhang
    Department of Biochemistry, Hadassah Medical School, Hebrew University, Jerusalem, Israel
    J Biol Chem 269:19573-7. 1994
    ..As histidine residues have been implicated in the mechanism of H+ transport in several systems, we propose that histidine 326 may be involved in the proton translocation accompanying sodium- and potassium-coupled glutamate transport...
  12. pmc The equivalent of a thallium binding residue from an archeal homolog controls cation interactions in brain glutamate transporters
    Shlomit Teichman
    Department of Biochemistry, Hebrew University Hadassah Medical School, Jerusalem 91120, Israel
    Proc Natl Acad Sci U S A 106:14297-302. 2009
    ..Thus the conserved aspartate is required for transporter-cation interactions in each of the two separate translocation steps and likely participates in an overlapping sodium and potassium binding site...
  13. ncbi request reprint The interaction of the gamma-aminobutyric acid transporter GAT-1 with the neurotransmitter is selectively impaired by sulfhydryl modification of a conformationally sensitive cysteine residue engineered into extracellular loop IV
    Elia Zomot
    Department of Biochemistry, Hadassah Medical School, The Hebrew University, Jerusalem 91120, Israel
    J Biol Chem 278:42950-8. 2003
    ..Moreover, GABA is unable to suppress the transient currents. Our results indicate that part of extracellular loop IV is conformationally sensitive, and its modification selectively abolishes the interaction of the transporter with GABA...
  14. ncbi request reprint Glutamate transporters from brain. A novel neurotransmitter transporter family
    B I Kanner
    Department of Biochemistry, Hadassah Medical School, Hebrew University, Jerusalem, Israel
    FEBS Lett 325:95-9. 1993
    ..Its transporter couples the flow of glutamate to that of sodium and potassium. Recently three different but related glutamate transporters have been cloned, which have no significant homology to the members of the superfamily...
  15. ncbi request reprint The aqueous accessibility in the external half of transmembrane domain I of the GABA transporter GAT-1 Is modulated by its ligands
    Yonggang Zhou
    Department of Biochemistry, Hadassah Medical School, The Hebrew University, Jerusalem 91120, Israel
    J Biol Chem 279:13800-8. 2004
    ..D. (2003) J. Biol. Chem. 278, 37052-37063), suggest that the extracellular part of TMD I is conformationally sensitive, lines the permeation pathway, and forms a more extended structure than expected from a membrane-embedded alpha-helix...
  16. ncbi request reprint The membrane topology of GAT-1, a (Na+ + Cl-)-coupled gamma-aminobutyric acid transporter from rat brain
    E R Bennett
    Department of Biochemistry, Hadassah Medical School, Hebrew University, Jerusalem, Israel
    J Biol Chem 272:1203-10. 1997
    ..It also contains 12 transmembrane segments, but the highly conserved domain 1 does not form a conventional transmembrane alpha-helix...
  17. ncbi request reprint Transporter-associated currents in the gamma-aminobutyric acid transporter GAT-1 are conditionally impaired by mutations of a conserved glycine residue
    Yonggang Zhou
    Department of Biochemistry, Hadassah Medical School, The Hebrew University, Jerusalem 91120, Israel
    J Biol Chem 280:20316-24. 2005
    ..Our results can be explained by a model invoking two outward-facing states of the empty transporter and a defective transition between these states in the glycine 80 mutants...
  18. ncbi request reprint Mechanism of chloride interaction with neurotransmitter:sodium symporters
    Elia Zomot
    Department of Biochemistry, Hebrew University Hadassah Medical School, POB 12272, Jerusalem 91120, Israel
    Nature 449:726-30. 2007
    ....
  19. ncbi request reprint Identification of a lithium interaction site in the gamma-aminobutyric acid (GABA) transporter GAT-1
    Yonggang Zhou
    Department of Biochemistry, Hebrew University Hadassah Medical School, P O Box 12272, Jerusalem 91120, Israel
    J Biol Chem 281:22092-9. 2006
    ..We propose that GABA participates in the other sodium binding site, just like leucine does in the Na1 site, and that at limiting GABA, this site determines the apparent sodium affinity of GABA transport...
  20. ncbi request reprint Cysteine-scanning mutagenesis reveals a conformationally sensitive reentrant pore-loop in the glutamate transporter GLT-1
    Myriam Grunewald
    Department of Biochemistry, Hadassah Medical School, The Hebrew University, Jerusalem 91120, Israel
    J Biol Chem 277:26074-80. 2002
    ..Our results indicate that reentrant loop II forms part of an aqueous pore, the access of which is blocked by the glutamate analogue dihydrokainate, and that sodium influences the conformation of this pore-loop...
  21. ncbi request reprint Cloning and expression of a rat brain L-glutamate transporter
    G Pines
    Department of Biochemistry, Hadassah Medical School, Hebrew University, Jerusalem, Israel
    Nature 360:464-7. 1992
    ..This protein therefore seems to be a member of a new family of transport molecules...
  22. ncbi request reprint Sulfhydryl modification of cysteine mutants of a neuronal glutamate transporter reveals an inverse relationship between sodium dependent conformational changes and the glutamate-gated anion conductance
    Liat Shachnai
    Department of Biochemistry, Hebrew University Hadassah Medical School, P O B 12272, Jerusalem 91120, Israel
    Neuropharmacology 49:862-71. 2005
    ..Sulfhydryl modification of I421C, but not of G283C, abolishes the sodium dependent transient currents. The results indicate the existence of multiple transitions between the coupled transport cycle and anion conducting states...
  23. ncbi request reprint Proximity of transmembrane domains 1 and 3 of the gamma-aminobutyric acid transporter GAT-1 inferred from paired cysteine mutagenesis
    Elia Zomot
    Department of Biochemistry, Hebrew University Hadassah Medical School, Jerusalem 91120, Israel
    J Biol Chem 280:25512-6. 2005
    ..The inhibition by cadmium was only observed when the cysteine pairs were introduced in the same polypeptide. Our results suggest that transmembrane domains 1 and 3 come in close proximity within the transporter monomer...
  24. ncbi request reprint Transmembrane domains I and II of the gamma-aminobutyric acid transporter GAT-4 contain molecular determinants of substrate specificity
    Nir Melamed
    Department of Biochemistry, Hadassah Medical School, The Hebrew University, Jerusalem, Israel
    Mol Pharmacol 65:1452-61. 2004
    ..The size of the side chain at positions 61 and 94 seems to determine the ability of substrate and substrate analogs to interact with the transporter...
  25. ncbi request reprint Dynamic equilibrium between coupled and uncoupled modes of a neuronal glutamate transporter
    Lars Borre
    Department of Biochemistry, Hadassah Medical School, The Hebrew University, P O Box 12272, Jerusalem 91120, Israel
    J Biol Chem 277:13501-7. 2002
    ..Thus, the modification of the cysteine introduced at position 421 impacts the coupled but not the uncoupled fluxes. Although both fluxes are activated by substrate, they behave as independent processes that are in dynamic equilibrium...
  26. pmc Transmembrane domain 8 of the {gamma}-aminobutyric acid transporter GAT-1 lines a cytoplasmic accessibility pathway into its binding pocket
    Assaf Ben-Yona
    Department of Biochemistry, Hebrew University Hadassah Medical School, P O Box 12272, Jerusalem 91120, Israel
    J Biol Chem 284:9727-32. 2009
    ....
  27. pmc A conserved methionine residue controls the substrate selectivity of a neuronal glutamate transporter
    Noa Rosental
    Department of Biochemistry and Molecular Biology, Institute for Medical Research Israel Canada, Hebrew University Hadassah Medical School, Jerusalem 91120, Israel
    J Biol Chem 285:21241-8. 2010
    ..Our results are consistent with the idea that the side chain of Met-367 fulfills a steric role in the positioning of the substrate in the binding pocket in a step subsequent to its initial binding...
  28. pmc Substrates and non-transportable analogues induce structural rearrangements at the extracellular entrance of the glial glutamate transporter GLT-1/EAAT2
    Shaogang Qu
    Department of Biochemistry, Hebrew University Hadassah Medical School, Jerusalem 91120, Israel
    J Biol Chem 283:26391-400. 2008
    ..Our results are consistent with the proposal that HP2 serves as the extracellular gate of the transporter and indicate that glutamate and the two analogues induce distinct conformations of HP2...
  29. doi request reprint The substrates of the gamma-aminobutyric acid transporter GAT-1 induce structural rearrangements around the interface of transmembrane domains 1 and 6
    Alex Rosenberg
    Department of Biochemistry, Hebrew University Hadassah Medical School, Jerusalem, Israel
    J Biol Chem 283:14376-83. 2008
    ..Thus, the degree of inhibition by CuPh is not a simple function of the accessibility of the individual cysteines but also involves structural rearrangements around the TM 1b/TM 6a interface...
  30. ncbi request reprint Arginine 445 controls the coupling between glutamate and cations in the neuronal transporter EAAC-1
    Lars Borre
    Department of Biochemistry, Hadassah Medical School, Hebrew University, Jerusalem 91120, Israel
    J Biol Chem 279:2513-9. 2004
    ..Therefore, it seems that the elimination of a positive charge in the vicinity of the substrate-binding site converts the transporter into a glutamate-gated cation-conducting pathway...
  31. ncbi request reprint Conformationally sensitive reactivity to permeant sulfhydryl reagents of cysteine residues engineered into helical hairpin 1 of the glutamate transporter GLT-1
    Irina Shlaifer
    Department of Biochemistry, Hebrew University Hadassah Medical School, Jerusalem, Israel
    Mol Pharmacol 71:1341-8. 2007
    ..According to this model, the inward movement of hairpin 1 results in the opening of a pathway between the binding pocket and the cytoplasm, lined by parts of transmembrane domains 7 and 8...
  32. ncbi request reprint Multiple consequences of mutating two conserved beta-bridge forming residues in the translocation cycle of a neuronal glutamate transporter
    Noa Rosental
    Department of Biochemistry, Hebrew University Hadassah Medical School, P O Box 12272, Jerusalem 91120, Israel
    J Biol Chem 281:27905-15. 2006
    ..On the other hand, the side chains of these residues themselves are required for the subsequent potassium relocation step...
  33. doi request reprint Structural biology: It's not all in the family
    Baruch I Kanner
    Nature 454:593-4. 2008
  34. ncbi request reprint Molecular physiology: intimate contact enables transport
    Baruch I Kanner
    Nature 437:203-5. 2005