J Tony Pembroke

Summary

Affiliation: University of Limerick
Country: Ireland

Publications

  1. pmc Control of expression of the ICE R391 encoded UV-inducible cell-sensitising function
    Patricia Armshaw
    Molecular and Structural Biochemistry Laboratory, Department of Chemical and Environmental Sciences, University of Limerick, Limerick, Ireland
    BMC Microbiol 13:195. 2013
  2. ncbi request reprint Isolation and analysis of a circular form of the IncJ conjugative transposon-like elements, R391 and R997: implications for IncJ incompatibility
    J T Pembroke
    Molecular Biochemistry Group, University of Limerick, Limerick, Ireland
    FEMS Microbiol Lett 187:133-8. 2000
  3. doi request reprint Introducing principles of validation into biochemistry and biotechnology courses: Practical concepts and a framework for active learning
    J T Pembroke
    Industrial Biochemistry Programme, Molecular and Structural Biochemistry Laboratory, University of Limerick, Limerick, Ireland
    Biochem Mol Biol Educ 36:70-5. 2008
  4. ncbi request reprint A novel ICE in the genome of Shewanella putrefaciens W3-18-1: comparison with the SXT/R391 ICE-like elements
    J Tony Pembroke
    Molecular and Structural Biochemistry Laboratory, Department of Chemical and Environmental Sciences, University of Limerick, Limerick, Ireland
    FEMS Microbiol Lett 264:80-8. 2006
  5. ncbi request reprint The role of conjugative transposons in the Enterobacteriaceae
    J T Pembroke
    Molecular Biochemistry Group, University of Limerick, Limerick, Ireland
    Cell Mol Life Sci 59:2055-64. 2002
  6. ncbi request reprint Pulsed field gel electrophoresis to rapidly detect the presence of IncJ conjugative transposon-like elements
    J T Pembroke
    Molecular Biochemistry Laboratory, Department of Chemical and Environmental Sciences, University of Limerick, Limerick, Ireland
    Lett Appl Microbiol 41:258-61. 2005
  7. ncbi request reprint The orf4 gene of the enterobacterial ICE, R391, encodes a novel UV-inducible recombination directionality factor, Jef, involved in excision and transfer of the ICE
    Barry M McGrath
    Molecular and Structural Biochemistry Laboratory, Department of Chemical and Environmental Sciences, University of Limerick, Limerick, Ireland
    FEMS Microbiol Lett 272:99-105. 2007
  8. ncbi request reprint Molecular tools to detect the IncJ elements: a family of integrating, antibiotic resistant mobile genetic elements
    Barry M McGrath
    Molecular Biochemistry Laboratory, Department of Chemical and Environmental Sciences, University of Limerick, Limerick, Ireland
    J Microbiol Methods 66:32-42. 2006
  9. ncbi request reprint Pre-exposure to UV irradiation increases the transfer frequency of the IncJ conjugative transposon-like elements R391, R392, R705, R706, R997 and pMERPH and is recA+ dependent
    Barry M McGrath
    Molecular Biochemistry Laboratory, Department of Chemical and Environmental Sciences, Industrial Biochemistry Programme, University of Limerick, Limerick, Ireland
    FEMS Microbiol Lett 243:461-5. 2005
  10. ncbi request reprint Detailed analysis of the insertion site of the mobile elements R997, pMERPH, R392, R705 and R391 in E. coli K12
    Barry M McGrath
    Biochemistry Laboratory, Department of Chemical and Environmental Sciences, University of Limerick, Limerick, Ireland
    FEMS Microbiol Lett 237:19-26. 2004

Collaborators

Detail Information

Publications22

  1. pmc Control of expression of the ICE R391 encoded UV-inducible cell-sensitising function
    Patricia Armshaw
    Molecular and Structural Biochemistry Laboratory, Department of Chemical and Environmental Sciences, University of Limerick, Limerick, Ireland
    BMC Microbiol 13:195. 2013
    ....
  2. ncbi request reprint Isolation and analysis of a circular form of the IncJ conjugative transposon-like elements, R391 and R997: implications for IncJ incompatibility
    J T Pembroke
    Molecular Biochemistry Group, University of Limerick, Limerick, Ireland
    FEMS Microbiol Lett 187:133-8. 2000
    ..This observation has implications for the nature of the incompatibility associated with IncJ elements and also provides a reliable method for isolating IncJ elements for molecular characterisation...
  3. doi request reprint Introducing principles of validation into biochemistry and biotechnology courses: Practical concepts and a framework for active learning
    J T Pembroke
    Industrial Biochemistry Programme, Molecular and Structural Biochemistry Laboratory, University of Limerick, Limerick, Ireland
    Biochem Mol Biol Educ 36:70-5. 2008
    ....
  4. ncbi request reprint A novel ICE in the genome of Shewanella putrefaciens W3-18-1: comparison with the SXT/R391 ICE-like elements
    J Tony Pembroke
    Molecular and Structural Biochemistry Laboratory, Department of Chemical and Environmental Sciences, University of Limerick, Limerick, Ireland
    FEMS Microbiol Lett 264:80-8. 2006
    ....
  5. ncbi request reprint The role of conjugative transposons in the Enterobacteriaceae
    J T Pembroke
    Molecular Biochemistry Group, University of Limerick, Limerick, Ireland
    Cell Mol Life Sci 59:2055-64. 2002
    ..Recent evidence suggests that in the Enterobacteriaceae the elements may be related to enteric pathogenicity islands. The evolution, distribution and role of these elements in the Enterobacteriaceae is discussed...
  6. ncbi request reprint Pulsed field gel electrophoresis to rapidly detect the presence of IncJ conjugative transposon-like elements
    J T Pembroke
    Molecular Biochemistry Laboratory, Department of Chemical and Environmental Sciences, University of Limerick, Limerick, Ireland
    Lett Appl Microbiol 41:258-61. 2005
    ..To develop a screening method to detect the presence of the IncJ group of integrating conjugative transposon-like elements upon transfer to Escherichia coli...
  7. ncbi request reprint The orf4 gene of the enterobacterial ICE, R391, encodes a novel UV-inducible recombination directionality factor, Jef, involved in excision and transfer of the ICE
    Barry M McGrath
    Molecular and Structural Biochemistry Laboratory, Department of Chemical and Environmental Sciences, University of Limerick, Limerick, Ireland
    FEMS Microbiol Lett 272:99-105. 2007
    ..orf4 is also conserved in other SXT/R391 family members such as R997, R392, R705 and pMERPH as shown by sequencing amplicons from these ICEs generated using orf4 specific primers...
  8. ncbi request reprint Molecular tools to detect the IncJ elements: a family of integrating, antibiotic resistant mobile genetic elements
    Barry M McGrath
    Molecular Biochemistry Laboratory, Department of Chemical and Environmental Sciences, University of Limerick, Limerick, Ireland
    J Microbiol Methods 66:32-42. 2006
    ..coli K12 strains to UV irradiation. A simple and rapid procedure for demonstrating this effect is described. These tools should enable the rapid detection of such elements in clinical and environmental settings...
  9. ncbi request reprint Pre-exposure to UV irradiation increases the transfer frequency of the IncJ conjugative transposon-like elements R391, R392, R705, R706, R997 and pMERPH and is recA+ dependent
    Barry M McGrath
    Molecular Biochemistry Laboratory, Department of Chemical and Environmental Sciences, Industrial Biochemistry Programme, University of Limerick, Limerick, Ireland
    FEMS Microbiol Lett 243:461-5. 2005
    ..Using real-time PCR, it was determined that the amount of the circular intermediate produced increased substantially upon UV irradiation...
  10. ncbi request reprint Detailed analysis of the insertion site of the mobile elements R997, pMERPH, R392, R705 and R391 in E. coli K12
    Barry M McGrath
    Biochemistry Laboratory, Department of Chemical and Environmental Sciences, University of Limerick, Limerick, Ireland
    FEMS Microbiol Lett 237:19-26. 2004
    ..The attB site is highly conserved and found in many different bacteria, suggesting a possible broad host range...
  11. doi request reprint Phylogenetic analysis of the bacterial community in a full scale autothermal thermophilic aerobic digester (ATAD) treating mixed domestic wastewater sludge for land spread
    Anna V Piterina
    Molecular Biochemistry Laboratory, Department of Chemical and Environmental Sciences, University of Limerick, Limerick, Ireland
    Water Res 46:2488-504. 2012
    ..The abundance of thermophilic, alkalophilic and cellulose-degrading phylotypes suggests that these organisms are responsible for maintaining the elevated temperature at the later stages of the ATAD process...
  12. doi request reprint Generation and analysis of an ICE R391 deletion library identifies genes involved in the element encoded UV-inducible cell-sensitising function
    Patricia Armshaw
    Department of Chemical and Environmental Sciences, Molecular and Structural Biochemistry Laboratory, University of Limerick, Limerick, Ireland
    FEMS Microbiol Lett 342:45-53. 2013
    ..We hypothesise that ICE R391 and other members of the SXT/R391 family display decreased survival rates upon exposure to UV irradiation through the induction of orf43...
  13. pmc Novel Tn4371-ICE like element in Ralstonia pickettii and genome mining for comparative elements
    Michael P Ryan
    Microbiology Laboratory, Department of Chemical and Environmental Sciences, University of Limerick, Limerick, Ireland
    BMC Microbiol 9:242. 2009
    ....
  14. pmc Genotypic and phenotypic diversity of Ralstonia pickettii and Ralstonia insidiosa isolates from clinical and environmental sources including High-purity Water. Diversity in Ralstonia pickettii
    Michael P Ryan
    Microbiology Laboratory, Department of Chemical and Environmental Sciences, University of Limerick, Limerick, Ireland
    BMC Microbiol 11:194. 2011
    ..This study compares the phenotypic and genotypic diversity of a selection of strains of Ralstonia collected from a variety of sources...
  15. doi request reprint Morphological characterisation of ATAD thermophilic sludge; sludge ecology and settleability
    Anna V Piterina
    Molecular Biochemistry Laboratory, Department of Chemical and Environmental Sciences, University of Limerick, Limerick, Ireland
    Water Res 45:3427-38. 2011
    ..Laser scanning confocal microscopy demonstrated a superior ability to identify composition and spatial localisation of structural constituents in such a dispersed, high water content sludge...
  16. pmc SXT/R391 Integrative and Conjugative Elements (ICEs) Encode a Novel 'Trap-Door' Strategy for Mobile Element Escape
    Michael P Ryan
    Molecular and Structural Biochemistry Group, Department of Chemical and Environmental Sciences, Materials and Surface Science Institute, University of Limerick Limerick, Ireland
    Front Microbiol 7:829. 2016
    ..This results in cell lysis and release of a circular form of the ICE. Induction of this novel system may allow transfer of an ICE, enhancing its survival potential under conditions not conducive to conjugative transfer. ..
  17. ncbi request reprint Isolation and characterisation of the ylmE homologue of Thermus thermophilus
    S Spada
    Industrial Biochemistry Group, Department of Chemical and Environmental Sciences, University of Limerick, Limerick, Ireland
    DNA Seq 11:507-14. 2001
    ..We speculate that the gene product may be involved in a cellular stress response in T. thermophilus...
  18. doi request reprint Examination of the cell sensitizing gene orf43 of ICE R391 suggests a role in ICE transfer enhancement to recipient cells
    Patricia Armshaw
    Molecular and Structural Biochemistry Laboratory, Materials and Surface Science Institute, Department of Chemical and Environmental Sciences, University of Limerick, Limerick, Ireland
    FEMS Microbiol Lett 362:1-7. 2015
    ..A hypothesis for retaining such a detrimental effect may be in its role of enhancing ICE survival upon cell damage. ..
  19. pmc C-NMR assessment of the pattern of organic matter transformation during domestic wastewater treatment by autothermal aerobic digestion (ATAD)
    Anna V Piterina
    Department of Chemical and Environmental Sciences, Material and Surface Science Institute, University of Limerick, Limerick, Ireland
    Int J Environ Res Public Health 6:2288-306. 2009
    ....
  20. ncbi request reprint Isolation of a novel Thermus thermophilus metal efflux protein that improves Escherichia coli growth under stress conditions
    Stefania Spada
    Department of Chemical and Environmental Sciences, University of Limerick, Plassey Technological Park, Limerick, Ireland
    Extremophiles 6:301-8. 2002
    ..The molecular basis and potential application of the findings are discussed...
  21. ncbi request reprint Cloning and characterisation of the Hint homologue of the thermophile Thermus thermophilus
    Y Gibert
    Industrial Biochemistry Group, Department of Chemical and Environmental Sciences, University of Limerick, Limerick, Ireland
    DNA Seq 12:179-85. 2001
    ..Here we report on the characterisation of the novel hint sequence...
  22. pmc R391: a conjugative integrating mosaic comprised of phage, plasmid, and transposon elements
    Dietmar Böltner
    Department of Biological Sciences, University of Essex, Colchester, United Kingdom
    J Bacteriol 184:5158-69. 2002
    ..Challenging the previous assumption that IncJ elements are plasmids, no plasmid replicon was identified on R391, suggesting that they cannot replicate autonomously...