Martin Caffrey

Summary

Affiliation: Trinity College
Country: Ireland

Publications

  1. pmc 'Hit and run' serial femtosecond crystallography of a membrane kinase in the lipid cubic phase
    Martin Caffrey
    Membrane Structural and Functional Biology Group, School of Medicine and School of Biochemistry and Immunology, Trinity College Dublin, Dublin 2, Republic of Ireland
    Philos Trans R Soc Lond B Biol Sci 369:20130621. 2014
  2. pmc Membrane protein structure determination using crystallography and lipidic mesophases: recent advances and successes
    Martin Caffrey
    Membrane Structural and Functional Biology Group, School of Medicine and School of Biochemistry and Immunology, Trinity College Dublin, Dublin, Ireland
    Biochemistry 51:6266-88. 2012
  3. pmc Crystallizing membrane proteins for structure-function studies using lipidic mesophases
    Martin Caffrey
    Membrane Structural and Functional Biology Group, School of Biochemistry and Immunology, and School of Medicine, Trinity College Dublin, Ireland
    Biochem Soc Trans 39:725-32. 2011
  4. pmc Crystal structure of the integral membrane diacylglycerol kinase
    Dianfan Li
    School of Biochemistry and Immunology and School of Medicine, Trinity College Dublin, Dublin 2, Ireland
    Nature 497:521-4. 2013
  5. pmc Crystallizing transmembrane peptides in lipidic mesophases
    Nicole Höfer
    Membrane Structural and Functional Biology Group, School of Biochemistry and Immunology, and School of Medicine, Trinity College, Dublin, Ireland
    Biophys J 99:L23-5. 2010
  6. pmc Ternary structure reveals mechanism of a membrane diacylglycerol kinase
    Dianfan Li
    School of Medicine and School of Biochemistry and Immunology, Trinity College Dublin, Dublin 2, Ireland
    Nat Commun 6:10140. 2015
  7. doi request reprint Structural basis of lipoprotein signal peptidase II action and inhibition by the antibiotic globomycin
    Lutz Vogeley
    School of Medicine and School of Biochemistry and Immunology, Trinity College Dublin, Dublin, Ireland
    Science 351:876-80. 2016
  8. pmc Lipid cubic phase as a membrane mimetic for integral membrane protein enzymes
    Dianfan Li
    Schools of Medicine and Biochemistry and Immunology, Trinity College, Dublin, Ireland
    Proc Natl Acad Sci U S A 108:8639-44. 2011
  9. pmc Structural basis for polyspecificity in the POT family of proton-coupled oligopeptide transporters
    Joseph A Lyons
    Schools of Medicine and Biochemistry and Immunology, Trinity College Dublin, Dublin, Ireland
    EMBO Rep 15:886-93. 2014
  10. pmc Experimental phasing for structure determination using membrane-protein crystals grown by the lipid cubic phase method
    Dianfan Li
    Membrane Structural and Functional Group, School of Medicine and School of Biochemistry and Immunology, Trinity College Dublin, Dublin, Ireland
    Acta Crystallogr D Biol Crystallogr 71:104-22. 2015

Collaborators

  • Valerie E Pye
  • Mark S P Sansom
  • Kay Diederichs
  • Petra Fromme
  • Sébastien Boutet
  • Wei Liu
  • Declan A Doyle
  • Simon Newstead
  • Colin P Kenyon
  • Dianfan Li
  • Lutz Vogeley
  • Joseph A Lyons
  • Coilin Boland
  • David Aragao
  • Chia Ying Huang
  • Nicole Howe
  • Phillip J Stansfeld
  • Toufic El Arnaout
  • Emmanuel Nji
  • Jingquan Tan
  • Syed T A Shah
  • Nicole Höfer
  • Meitian Wang
  • Ezequiel Panepucci
  • Jonathan Bailey
  • Vincent Olieric
  • Brian Kobilka
  • Rangana Warshamanage
  • Pikyee Ma
  • Xiangyu Liu
  • Tobias Weinert
  • Niall J English
  • Nadia A Zatsepin
  • Anton Barty
  • Marc Messerschmidt
  • Christopher Kupitz
  • Henry N Chapman
  • Aaron Keogh
  • Vadim Cherezov
  • Shibom Basu
  • Garth J Williams
  • Sateesh Bandaru
  • Kimberley Rendek
  • Ingo Grotjohann
  • M Marvin Seibert
  • Cornelius Gati
  • Uwe Weierstall
  • Oleksandr Yefanov
  • Raimund Fromme
  • Joanne L Parker
  • Alette R Brinth
  • Syed Tasadaque Ali Shah
  • Nicolae Solcan
  • Volker Dötsch
  • Stefan Haberstock
  • Frank Bernhard
  • John C Whitney
  • P Lynne Howell
  • Sarah L Rouse
  • Alette Brinth
  • Margaret Aherne
  • Christine Doherty

Detail Information

Publications20

  1. pmc 'Hit and run' serial femtosecond crystallography of a membrane kinase in the lipid cubic phase
    Martin Caffrey
    Membrane Structural and Functional Biology Group, School of Medicine and School of Biochemistry and Immunology, Trinity College Dublin, Dublin 2, Republic of Ireland
    Philos Trans R Soc Lond B Biol Sci 369:20130621. 2014
    ..Here, we describe the physico-chemical bases for these challenges, solutions to them and prospects for future uses of lipidic mesophases in the SFX arena. ..
  2. pmc Membrane protein structure determination using crystallography and lipidic mesophases: recent advances and successes
    Martin Caffrey
    Membrane Structural and Functional Biology Group, School of Medicine and School of Biochemistry and Immunology, Trinity College Dublin, Dublin, Ireland
    Biochemistry 51:6266-88. 2012
    ..In this work, these assorted advances are outlined along with a summary of the membrane proteins that have yielded to the method. The prospects for and the challenges that must be overcome to further develop the method are described...
  3. pmc Crystallizing membrane proteins for structure-function studies using lipidic mesophases
    Martin Caffrey
    Membrane Structural and Functional Biology Group, School of Biochemistry and Immunology, and School of Medicine, Trinity College Dublin, Ireland
    Biochem Soc Trans 39:725-32. 2011
    ..In the present article, these assorted advances are outlined, along with a summary of the membrane proteins that have yielded to the method. The challenges that must be overcome to develop the method further are described...
  4. pmc Crystal structure of the integral membrane diacylglycerol kinase
    Dianfan Li
    School of Biochemistry and Immunology and School of Medicine, Trinity College Dublin, Dublin 2, Ireland
    Nature 497:521-4. 2013
    ..They are, however, at variance with a published solution NMR model in that domain swapping, a key feature of the solution form, is not observed in the crystal structures...
  5. pmc Crystallizing transmembrane peptides in lipidic mesophases
    Nicole Höfer
    Membrane Structural and Functional Biology Group, School of Biochemistry and Immunology, and School of Medicine, Trinity College, Dublin, Ireland
    Biophys J 99:L23-5. 2010
    ..This result suggests that the in meso method should be considered as a viable means for high-resolution structure determination of integral membrane peptides, many of which are predicted to be coded for in the human genome...
  6. pmc Ternary structure reveals mechanism of a membrane diacylglycerol kinase
    Dianfan Li
    School of Medicine and School of Biochemistry and Immunology, Trinity College Dublin, Dublin 2, Ireland
    Nat Commun 6:10140. 2015
    ..A catalytic mechanism for this unique enzyme is proposed. The active site architecture shows clear evidence of having arisen by convergent evolution. ..
  7. doi request reprint Structural basis of lipoprotein signal peptidase II action and inhibition by the antibiotic globomycin
    Lutz Vogeley
    School of Medicine and School of Biochemistry and Immunology, Trinity College Dublin, Dublin, Ireland
    Science 351:876-80. 2016
    ..In an example of molecular mimicry, globomycin appears to inhibit by acting as a noncleavable peptide that sterically blocks the active site. This structure should inform rational antibiotic drug discovery. ..
  8. pmc Lipid cubic phase as a membrane mimetic for integral membrane protein enzymes
    Dianfan Li
    Schools of Medicine and Biochemistry and Immunology, Trinity College, Dublin, Ireland
    Proc Natl Acad Sci U S A 108:8639-44. 2011
    ..Because of its open, bicontinuous nature, the cubic phase offers the added benefit that the protein is accessible for assay from both sides of the membrane...
  9. pmc Structural basis for polyspecificity in the POT family of proton-coupled oligopeptide transporters
    Joseph A Lyons
    Schools of Medicine and Biochemistry and Immunology, Trinity College Dublin, Dublin, Ireland
    EMBO Rep 15:886-93. 2014
    ....
  10. pmc Experimental phasing for structure determination using membrane-protein crystals grown by the lipid cubic phase method
    Dianfan Li
    Membrane Structural and Functional Group, School of Medicine and School of Biochemistry and Immunology, Trinity College Dublin, Dublin, Ireland
    Acta Crystallogr D Biol Crystallogr 71:104-22. 2015
    ..The various techniques and strategies described will provide a valuable reference for future experimental phasing of membrane proteins where crystals are grown by the lipid cubic phase method. ..
  11. pmc A conformational landscape for alginate secretion across the outer membrane of Pseudomonas aeruginosa
    Jingquan Tan
    Schools of Medicine and Biochemistry and Immunology, Trinity College, Dublin, Ireland
    Acta Crystallogr D Biol Crystallogr 70:2054-68. 2014
    ..Alginate can thread through a continuous pore in the complex, suggesting that AlgK pre-orients newly synthesized exopolysaccharide for delivery to AlgE. ..
  12. pmc In meso in situ serial X-ray crystallography of soluble and membrane proteins at cryogenic temperatures
    Chia Ying Huang
    Membrane Structural and Functional Biology Group, School of Medicine and School of Biochemistry and Immunology, Trinity College, Dublin 2, D02 R590, Ireland
    Acta Crystallogr D Struct Biol 72:93-112. 2016
    ..Insulin and lysozyme were used as test soluble proteins. The quality of the data that can be generated by this method was attested to by performing sulfur and bromine SAD phasing with two of the test proteins. ..
  13. pmc Cell-free expression and in meso crystallisation of an integral membrane kinase for structure determination
    Coilin Boland
    Membrane Structural and Functional Group, School of Medicine and School of Biochemistry and Immunology, Trinity College Dublin, Dublin, Ireland
    Cell Mol Life Sci 71:4895-910. 2014
    ....
  14. pmc Cloning, expression, purification, crystallization and preliminary X-ray diffraction of a lysine-specific permease from Pseudomonas aeruginosa
    Emmanuel Nji
    Membrane Structural and Functional Biology Group, School of Medicine and School of Biochemistry and Immunology, Trinity College Dublin, Dublin, Ireland
    Acta Crystallogr F Struct Biol Commun 70:1362-7. 2014
    ..5 Å resolution. The diffraction data were indexed in space group P21, with unit-cell parameters a = 169.53, b = 169.53, c = 290.13 Å, γ = 120°...
  15. pmc Renaturing membrane proteins in the lipid cubic phase, a nanoporous membrane mimetic
    Dianfan Li
    School of Biochemistry and Immunology and School of Medicine, Trinity College Dublin, Dublin, Ireland
    Sci Rep 4:5806. 2014
    ..55 Å. This highly efficient, inexpensive, simple and rapid approach should find application wherever properly folded, membrane reconstituted and functional proteins are required where the starting material is a denatured aggregate. ..
  16. pmc Crystallizing membrane proteins using lipidic mesophases
    Martin Caffrey
    Membrane Structural and Functional Biology Group, University of Limerick, Limerick, Ireland
    Nat Protoc 4:706-31. 2009
    ..Methods for harvesting microcrystals are also described. The time required to prepare the protein-loaded mesophase and to set up a crystallization plate manually is about 1 h...