Philip M Cummins

Summary

Affiliation: Dublin City University
Country: Ireland

Publications

  1. ncbi request reprint Ion-Exchange Chromatography: Basic Principles and Application
    Philip M Cummins
    School of Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland
    Methods Mol Biol 1485:209-223. 2017
  2. ncbi request reprint Cyclic strain-mediated matrix metalloproteinase regulation within the vascular endothelium: a force to be reckoned with
    Philip M Cummins
    Vascular Health Research Centre, Faculty of Science and Health, Dublin City Univ, Dublin, Ireland
    Am J Physiol Heart Circ Physiol 292:H28-42. 2007
  3. pmc Occludin: one protein, many forms
    Philip M Cummins
    School of Biotechnology and Centre for Preventive Medicine, Dublin City University, Dublin, Ireland
    Mol Cell Biol 32:242-50. 2012
  4. ncbi request reprint Regulation of bovine brain microvascular endothelial tight junction assembly and barrier function by laminar shear stress
    Olga C Colgan
    Vascular Health Research Centre, Dublin City University, Glasnevin, Dublin 9, Ireland
    Am J Physiol Heart Circ Physiol 292:H3190-7. 2007
  5. ncbi request reprint Cyclic strain-mediated regulation of vascular endothelial cell migration and tube formation
    Nicholas Von Offenberg Sweeney
    Vascular Health Research Centre, Faculty of Science and Health, Dublin City University, Dublin 9, Ireland
    Biochem Biophys Res Commun 329:573-82. 2005
  6. doi request reprint Helicobacter pylori-induced inhibition of vascular endothelial cell functions: a role for VacA-dependent nitric oxide reduction
    Nicholas P Tobin
    School of Biotechnology, Dublin City University, Dublin, Ireland
    Am J Physiol Heart Circ Physiol 295:H1403-13. 2008
  7. ncbi request reprint Notch-mediated CBF-1/RBP-J{kappa}-dependent regulation of human vascular smooth muscle cell phenotype in vitro
    David Morrow
    Vascular Health Research Centre, Faculty of Science and Health, Dublin City University, Dublin 9, Ireland
    Am J Physiol Cell Physiol 289:C1188-96. 2005
  8. doi request reprint Influence of basolateral condition on the regulation of brain microvascular endothelial tight junction properties and barrier function
    Olga C Colgan
    Faculty of Science and Health, School of Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland
    Brain Res 1193:84-92. 2008
  9. doi request reprint Shear stress is a positive regulator of thimet oligopeptidase (EC3.4.24.15) in vascular endothelial cells: consequences for MHC1 levels
    Anthony F Guinan
    School of Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland
    Cardiovasc Res 99:545-54. 2013
  10. ncbi request reprint Cyclic strain inhibits Notch receptor signaling in vascular smooth muscle cells in vitro
    David Morrow
    Vascular Health Research Centre, Faculty of Science and Health, Dublin City University, Dublin, Ireland
    Circ Res 96:567-75. 2005

Collaborators

  • E M Redmond
  • Dermot Walls
  • Ciarán O'Fágáin
  • Steven W Kerrigan
  • Keith D Rochfort
  • Ronan P Murphy
  • Paul A Cahill
  • Yvonne A Birney
  • Colin Davenport
  • Alisha McLoughlin
  • David Morrow
  • Hannah Forde
  • Emma Harper
  • Anthony F Guinan
  • Nicholas Von Offenberg Sweeney
  • Fiona A Martin
  • Olga C Colgan
  • Tony G Walsh
  • Diarmuid Smith
  • Paul A Fitzpatrick
  • Laura E Collins
  • Catherine Sweeney
  • Nora T Collins
  • Nicholas P Tobin
  • Brendan F O'Connor
  • Robert Wallace
  • Ciaran M McGinn
  • Robert G Wallace
  • Gail Ferguson
  • Eoin J Cotter
  • Adrian R Pierotti
  • Ronan Murphy
  • Shaunta Guha
  • Agnieszka Scheller
  • Brian F MacDonnell
  • Laura C Twomey
  • Chun Xu Shan
  • Marco Mangone
  • Cormac J McDonnell
  • Niall Moyna
  • Marc Antoine Custaud
  • Susan Phelan
  • Paul Fitzpatrick
  • Andrew Murphy
  • Maria T Killeen
  • Gary T Henehan
  • John C Atherton
  • Dermot Cox
  • Gerardeane Meade
  • Nora Collins
  • Gerardene Meade
  • Seamus Coyle
  • Marc J Glucksman
  • James V Sitzmann
  • Catherine H Sweeney
  • Colm Hennessy
  • Paul M Coen
  • John P Cullen
  • Charles R Cappadona

Detail Information

Publications39

  1. ncbi request reprint Ion-Exchange Chromatography: Basic Principles and Application
    Philip M Cummins
    School of Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland
    Methods Mol Biol 1485:209-223. 2017
    ..Protocols for assaying total protein and enzyme activity in both pre- and post-IEC fractions are also described...
  2. ncbi request reprint Cyclic strain-mediated matrix metalloproteinase regulation within the vascular endothelium: a force to be reckoned with
    Philip M Cummins
    Vascular Health Research Centre, Faculty of Science and Health, Dublin City Univ, Dublin, Ireland
    Am J Physiol Heart Circ Physiol 292:H28-42. 2007
    ..Possible relevance of these phenomena to vascular endothelial dysfunction and pathological remodeling are also addressed...
  3. pmc Occludin: one protein, many forms
    Philip M Cummins
    School of Biotechnology and Centre for Preventive Medicine, Dublin City University, Dublin, Ireland
    Mol Cell Biol 32:242-50. 2012
    ..Mechanistic concepts such as splice variance and alternate promoter usage, proteolysis, phosphorylation, dimerization, and ubiquitination are comprehensively examined, and possible avenues for future investigation highlighted...
  4. ncbi request reprint Regulation of bovine brain microvascular endothelial tight junction assembly and barrier function by laminar shear stress
    Olga C Colgan
    Vascular Health Research Centre, Dublin City University, Glasnevin, Dublin 9, Ireland
    Am J Physiol Heart Circ Physiol 292:H3190-7. 2007
    ..Shear stress upregulates BBMvEC barrier function at the molecular level via modulation of expression, association, and localization of occludin and ZO-1. The pulsatile shear model appeared to give the most profound biochemical responses...
  5. ncbi request reprint Cyclic strain-mediated regulation of vascular endothelial cell migration and tube formation
    Nicholas Von Offenberg Sweeney
    Vascular Health Research Centre, Faculty of Science and Health, Dublin City University, Dublin 9, Ireland
    Biochem Biophys Res Commun 329:573-82. 2005
    ..7+/-31%, respectively. Finally, integrin blockade with cRGD peptide and siRNA-directed attenuation of uPA expression reduced strain-induced tube formation by 85.7+/-15% and 84.7+/-31%, respectively, whilst having no effect on migration...
  6. doi request reprint Helicobacter pylori-induced inhibition of vascular endothelial cell functions: a role for VacA-dependent nitric oxide reduction
    Nicholas P Tobin
    School of Biotechnology, Dublin City University, Dublin, Ireland
    Am J Physiol Heart Circ Physiol 295:H1403-13. 2008
    ..Specifically, the VacA-dependent reduction in endothelial NO is indicated in these events. The atheroprotective impact of laminar shear stress in this context is also evident...
  7. ncbi request reprint Notch-mediated CBF-1/RBP-J{kappa}-dependent regulation of human vascular smooth muscle cell phenotype in vitro
    David Morrow
    Vascular Health Research Centre, Faculty of Science and Health, Dublin City University, Dublin 9, Ireland
    Am J Physiol Cell Physiol 289:C1188-96. 2005
    ....
  8. doi request reprint Influence of basolateral condition on the regulation of brain microvascular endothelial tight junction properties and barrier function
    Olga C Colgan
    Faculty of Science and Health, School of Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland
    Brain Res 1193:84-92. 2008
    ....
  9. doi request reprint Shear stress is a positive regulator of thimet oligopeptidase (EC3.4.24.15) in vascular endothelial cells: consequences for MHC1 levels
    Anthony F Guinan
    School of Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland
    Cardiovasc Res 99:545-54. 2013
    ..We examine for the first time the relationship between TOP expression and laminar shear stress, a haemodynamic force associated with endothelium-mediated vascular homeostasis...
  10. ncbi request reprint Cyclic strain inhibits Notch receptor signaling in vascular smooth muscle cells in vitro
    David Morrow
    Vascular Health Research Centre, Faculty of Science and Health, Dublin City University, Dublin, Ireland
    Circ Res 96:567-75. 2005
    ..These findings suggest that cyclic strain inhibits SMC growth while enhancing SMC apoptosis, in part, through regulation of Notch receptor and downstream target gene expression...
  11. doi request reprint Down-regulation of neprilysin (EC3.4.24.11) expression in vascular endothelial cells by laminar shear stress involves NADPH oxidase-dependent ROS production
    Paul A Fitzpatrick
    School of Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland
    Int J Biochem Cell Biol 41:2287-94. 2009
    ..In conclusion, we confirm for the first time that NEP expression is down-regulated in vascular endothelial cells by physiological laminar shear, possibly via a mechanotransduction mechanism involving NADPH oxidase-induced ROS production...
  12. ncbi request reprint Biomechanical regulation of hedgehog signaling in vascular smooth muscle cells in vitro and in vivo
    David Morrow
    Faculty of Science and Health, Vascular Health Research Centre, Dublin City University, Glasnevin, Dublin 9, Ireland
    Am J Physiol Cell Physiol 292:C488-96. 2007
    ..These data suggest that Hh expression is diminished by biomechanical stimulation in vitro and in vivo and thus may play a fundamental role in arterial remodeling and atherogenesis in vivo...
  13. doi request reprint Stabilization of brain microvascular endothelial barrier function by shear stress involves VE-cadherin signaling leading to modulation of pTyr-occludin levels
    Tony G Walsh
    School of Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland
    J Cell Physiol 226:3053-63. 2011
    ..A downstream role is also strongly indicated for a protein tyrosine phosphatase in pTyr-occludin modulation. Importantly, these findings suggest an important route of inter-junctional signaling cross-talk during BBB response to flow...
  14. ncbi request reprint Cyclic strain-mediated regulation of vascular endothelial occludin and ZO-1: influence on intercellular tight junction assembly and function
    Nora T Collins
    Vascular Health Research Centre, Faculty of Science and Health, Dublin City University, Glasnevin, Dublin 9, Ireland
    Arterioscler Thromb Vasc Biol 26:62-8. 2006
    ..We, therefore, examined the effects of cyclic strain on the expression, modification, and function of 2 pivotal endothelial tight junction components, occludin and ZO-1...
  15. ncbi request reprint Cyclic strain-mediated regulation of endothelial matrix metalloproteinase-2 expression and activity
    Nicholas Von Offenberg Sweeney
    Vascular Health Research Centre, Faculty of Science and Health, Dublin City University, Glasnevin, Dublin 9, Ireland
    Cardiovasc Res 63:625-34. 2004
    ..To investigate the role of cyclic strain in controlling matrix metalloproteinase-2 (MMP-2) expression and activity in endothelial cells (ECs) in vitro...
  16. doi request reprint RANKL promotes osteoblastic activity in vascular smooth muscle cells by upregulating endothelial BMP-2 release
    Colin Davenport
    School of Biotechnology, Dublin City University, Dublin, Ireland Electronic address
    Int J Biochem Cell Biol 77:171-80. 2016
    ....
  17. ncbi request reprint Pulse pressure-induced transmural fluid flux increases bovine aortic smooth muscle cell apoptosis in a mitogen activated protein kinase dependent manner
    Yvonne A Birney
    Vascular Health Research Centre, Faculty of Science and Health, Dublin City University, Dublin, Ireland
    J Vasc Res 41:364-74. 2004
    ..We investigated the effect of pulse pressure on VSMC apoptosis...
  18. ncbi request reprint Cyclic strain-induced endothelial MMP-2: role in vascular smooth muscle cell migration
    Nicholas Von Offenberg Sweeney
    Vascular Health Research Centre, Faculty of Science and Health, Dublin City University, Glasnevin, Dublin 9, Ireland
    Biochem Biophys Res Commun 320:325-33. 2004
    ..These findings reveal a potentially novel role for strain-induced endothelial MMP-2 in regulating vascular SMC migration...
  19. doi request reprint Vascular calcification in type-2 diabetes and cardiovascular disease: Integrative roles for OPG, RANKL and TRAIL
    Emma Harper
    School of Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland Electronic address
    Vascul Pharmacol 82:30-40. 2016
    ..In this regard, a clear focus on VC as it applies to T2DM and cardiovascular disease (and particularly atherosclerosis) will be maintained. ..
  20. ncbi request reprint Notch 1 and 3 receptor signaling modulates vascular smooth muscle cell growth, apoptosis, and migration via a CBF-1/RBP-Jk dependent pathway
    Catherine Sweeney
    Vascular Health Research Centre, Faculty of Science and Health, Dublin City University, Dublin 9, Ireland
    FASEB J 18:1421-3. 2004
    ..Notch signaling, therefore, represents a novel therapeutic target for disease states in which changes in vascular cell fate occur in vivo...
  21. doi request reprint The beneficial pleiotropic effects of tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) within the vasculature: A review of the evidence
    Hannah Forde
    Department of Academic Endocrinology and Diabetes, Royal College of Surgeons Medical School and Beaumont Hospital, Dublin 9, Ireland School of Biotechnology, Dublin City University, Dublin 9, Ireland Electronic address
    Atherosclerosis 247:87-96. 2016
    ..To this end, extensive in vitro, in vivo, and clinical studies will be reviewed and critical findings highlighted...
  22. doi request reprint Thrombomodulin and the vascular endothelium: insights into functional, regulatory, and therapeutic aspects
    Fiona A Martin
    School of Biotechnology, Dublin City University, Dublin, Ireland
    Am J Physiol Heart Circ Physiol 304:H1585-97. 2013
    ....
  23. ncbi request reprint Regulation of endopeptidases EC3.4.24.15 and EC3.4.24.16 in vascular endothelial cells by cyclic strain: role of Gi protein signaling
    Eoin J Cotter
    Vascular Health Research Centre, Faculty of Science and Health, Dublin City University, Glasnevin, Dublin, Ireland
    Arterioscler Thromb Vasc Biol 24:457-63. 2004
    ..The objective of this study, therefore, was to investigate the putative role of cyclic strain in regulating the expression and enzymatic activity of EP24.15 and EP24.16 in bovine aortic endothelial cells (BAECs)...
  24. doi request reprint Hydrophobic interaction chromatography
    Philip M Cummins
    School of Biotechnology, Dublin City University, Dublin, Ireland
    Methods Mol Biol 681:431-7. 2011
    ..For these reasons, this technique has a widespread use for the purification of proteins and large polypeptides...
  25. ncbi request reprint Hemodynamic regulation of metallopeptidases within the vasculature
    Philip M Cummins
    Vascular Health Research Centre, Dublin City University, Glasnevin, Dublin 9, Ireland
    Protein Pept Lett 11:433-42. 2004
    ..This review examines the relationship between hemodynamic forces such as shear stress and cyclic strain, and an important group of peptide-degrading enzymes within the endothelium, the thermolysin-like zinc metallopeptidases...
  26. doi request reprint Staphylococcus aureus-mediated blood-brain barrier injury: an in vitro human brain microvascular endothelial cell model
    Alisha McLoughlin
    School of Biotechnology, Dublin City University, Dublin, Ireland
    Cell Microbiol . 2016
    ..In conclusion, these findings provide insight into how SA infection may activate proinflammatory mechanisms within the brain microvascular endothelium to elicit BBB failure...
  27. doi request reprint Tumour necrosis factor-α-mediated disruption of cerebrovascular endothelial barrier integrity in vitro involves the production of proinflammatory interleukin-6
    Keith D Rochfort
    School of Biotechnology, Dublin City University, Glasnevin, Dublin, Ireland
    J Neurochem 136:564-72. 2016
    ..These findings provide insight into the complex nature of proinflammatory cytokine injury at the BBB microvascular endothelium interface...
  28. pmc Shear-dependent attenuation of cellular ROS levels can suppress proinflammatory cytokine injury to human brain microvascular endothelial barrier properties
    Keith D Rochfort
    School of Biotechnology, Dublin City University, Glasnevin, Dublin, Ireland
    J Cereb Blood Flow Metab 35:1648-56. 2015
    ....
  29. pmc Regulation of thrombomodulin expression and release in human aortic endothelial cells by cyclic strain
    Fiona A Martin
    School of Biotechnology, Dublin City University, Glasnevin, Dublin, Ireland
    PLoS ONE 9:e108254. 2014
    ..Much is still unknown however about how endothelial TM is regulated by physiologic hemodynamic forces (and particularly cyclic strain) intrinsic to endothelial-mediated vascular homeostasis...
  30. ncbi request reprint Gel-Filtration Chromatography
    Ciarán O'Fágáin
    School of Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland
    Methods Mol Biol 1485:15-25. 2017
    ..This article describes the basis of the method, the selection of suitable operating conditions, and contrasts typical matrix types. Applications of the technique are described, with references to the scientific literature...
  31. doi request reprint Ion-exchange chromatography: basic principles and application to the partial purification of soluble mammalian prolyl oligopeptidase
    Philip M Cummins
    School of Biotechnology, Dublin City University, Dublin, Ireland
    Methods Mol Biol 681:215-28. 2011
    ..g. thyroliberin, gonadotropin-releasing hormone)...
  32. doi request reprint Thrombomodulin regulation in human brain microvascular endothelial cells in vitro: role of cytokines and shear stress
    Keith D Rochfort
    School of Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland Centre for Preventive Medicine, Dublin City University, Glasnevin, Dublin 9, Ireland
    Microvasc Res 97:1-5. 2015
    ..In summary, our data indicate that TM is differentially regulated within cultured HBMvECs by humoral and biomechanical signals. ..
  33. pmc Downregulation of blood-brain barrier phenotype by proinflammatory cytokines involves NADPH oxidase-dependent ROS generation: consequences for interendothelial adherens and tight junctions
    Keith D Rochfort
    School of Biotechnology, Dublin City University, Dublin, Ireland
    PLoS ONE 9:e101815. 2014
    ....
  34. ncbi request reprint Hydrophobic Interaction Chromatography
    Brendan F O'Connor
    School of Biotechnology, Dublin City University, Dublin 9, Republic of Ireland
    Methods Mol Biol 1485:355-363. 2017
    ..For these reasons, this technique has a widespread use for the purification of proteins and large polypeptides...
  35. pmc Potential Diagnostic and Prognostic Biomarkers of Epigenetic Drift within the Cardiovascular Compartment
    Robert G Wallace
    School of Health and Human Performance, Dublin City University, Ireland
    Biomed Res Int 2016:2465763. 2016
    ..Platelets release increased levels of MVs in response to various stimuli and under a plethora of disease states, which demonstrate a functional effect on other cell types. ..
  36. doi request reprint The blood-brain barrier endothelium: a target for pro-inflammatory cytokines
    Keith D Rochfort
    School of Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland
    Biochem Soc Trans 43:702-6. 2015
    ..The mechanistic role of nicotinamide adenine dinucleotide phosphate oxidase (NADPH oxidase)-induced oxidative stress in these events will also be addressed. ..
  37. doi request reprint Cytokine-mediated dysregulation of zonula occludens-1 properties in human brain microvascular endothelium
    Keith D Rochfort
    School of Biotechnology, Centre for Preventive Medicine, Dublin City University, Glasnevin, Dublin 9, Ireland
    Microvasc Res 100:48-53. 2015
    ..A causal role for NADPH oxidase activation and oxidant signalling is also confirmed. Our findings add mechanistic depth to current in vivo models of BBB injury manifesting ZO-1 dysregulation...
  38. ncbi request reprint Microparticles: A Pivotal Nexus in Vascular Homeostasis and Disease
    Ciaran M McGinn
    School of Health and Human Performance, Dublin City University, Glasnevin, Dublin 9, Ireland
    Curr Clin Pharmacol 11:28-42. 2016
    ..This review synopsizes our current understanding of the role of MPs in cardiovascular disease, their biogenesis and effector function, and their future use as both diagnostic and prognostic indices of cardiovascular disease. ..
  39. doi request reprint Gel-filtration chromatography
    Ciarán O'Fágáin
    School of Biotechnology and National Centre for Sensor Research, Dublin City University, Dublin, Ireland
    Methods Mol Biol 681:25-33. 2011
    ..Here, the basis of the method is described and typical matrix types are contrasted. The selection of suitable operating conditions and applications of the method are also discussed...