Mohammad Hossein Nasr-Esfahani

Summary

Affiliation: Isfahan University of Medical Sciences
Country: Iran

Publications

  1. ncbi request reprint Relationship between protamine deficiency with fertilization rate and incidence of sperm premature chromosomal condensation post-ICSI
    M H Nasr-Esfahani
    Royan Institute, Tehran, Iran
    Andrologia 36:95-100. 2004
  2. doi request reprint Improved bovine ICSI outcomes by sperm selected after combined heparin-glutathione treatment
    Mohammad Hadi Sekhavati
    Department of Animal Science, Ferdowsi University of Mashhad, Mashhad, Iran
    Cell Reprogram 14:295-304. 2012
  3. ncbi request reprint Sperm selection for ICSI using the hyaluronic acid binding assay
    Mohammad Hossein Nasr-Esfahani
    Department of Reproduction and Development, Reproductive Biomedicine Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran
    Methods Mol Biol 927:263-8. 2013
  4. doi request reprint Artificial oocyte activation and intracytoplasmic sperm injection
    Mohammad Hossein Nasr-Esfahani
    Isfahan Fertility and Infertility Center, Isfahan, Iran
    Fertil Steril 94:520-6. 2010
  5. pmc Evaluation of sperm selection procedure based on hyaluronic acid binding ability on ICSI outcome
    M H Nasr-Esfahani
    Department of Embryology and Andrology, Royan Institute, P O Box 19395 4644, Tehran, Iran
    J Assist Reprod Genet 25:197-203. 2008
  6. doi request reprint Artificial oocyte activation in severe teratozoospermia undergoing intracytoplasmic sperm injection
    Mohammad H Nasr-Esfahani
    Department of Embryology and Andrology, Royan Institute, Tehran, Iran
    Fertil Steril 90:2231-7. 2008
  7. ncbi request reprint Failed fertilization after ICSI and spermiogenic defects
    Mohammad H Nasr-Esfahani
    Department of Embryology and Andrology, Royan Institute, Tehran, Iran
    Fertil Steril 89:892-8. 2008
  8. ncbi request reprint Effects of failed oocyte activation and sperm protamine deficiency on fertilization post-ICSI
    Mohammad Hossein Nasr-Esfahani
    Department of Embryology and Andrology, Royan Institute, Tehran, Iran
    Reprod Biomed Online 14:422-9. 2007
  9. ncbi request reprint Can sperm protamine deficiency induce sperm premature chromosomal condensation?
    M H Nasr-Esfahani
    Department of Embryology, Royan Institute, Tehran, Iran
    Andrologia 38:92-8. 2006
  10. ncbi request reprint Varicocelectomy: semen parameters and protamine deficiency
    M H Nasr-Esfahani
    Department of Embryology and Andrology, Royan Institute, Tehran, Iran
    Int J Androl 32:115-22. 2009

Collaborators

Detail Information

Publications85

  1. ncbi request reprint Relationship between protamine deficiency with fertilization rate and incidence of sperm premature chromosomal condensation post-ICSI
    M H Nasr-Esfahani
    Royan Institute, Tehran, Iran
    Andrologia 36:95-100. 2004
    ..05). A significant correlation was observed between sperm protamine deficiency with fertilization rate. Hence sperm protamine deficiency affects fertilization rate and possibly prones sperm to PCC post-ICSI...
  2. doi request reprint Improved bovine ICSI outcomes by sperm selected after combined heparin-glutathione treatment
    Mohammad Hadi Sekhavati
    Department of Animal Science, Ferdowsi University of Mashhad, Mashhad, Iran
    Cell Reprogram 14:295-304. 2012
    ..05). The results of this study suggested that the degree of sperm decondensation derived from heparin-GSH pretreatment may affect ICSI efficiency in bovine...
  3. ncbi request reprint Sperm selection for ICSI using the hyaluronic acid binding assay
    Mohammad Hossein Nasr-Esfahani
    Department of Reproduction and Development, Reproductive Biomedicine Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran
    Methods Mol Biol 927:263-8. 2013
    ..The methodology explained below describes the selection of sperm based on the ability of sperm to bind solid-state hyaluronic acid as its receptor, present on mature sperm with intact chromatin...
  4. doi request reprint Artificial oocyte activation and intracytoplasmic sperm injection
    Mohammad Hossein Nasr-Esfahani
    Isfahan Fertility and Infertility Center, Isfahan, Iran
    Fertil Steril 94:520-6. 2010
    ..To review different methods for artificial oocyte activation and its impact on intracytoplasmic sperm injection (ICSI)...
  5. pmc Evaluation of sperm selection procedure based on hyaluronic acid binding ability on ICSI outcome
    M H Nasr-Esfahani
    Department of Embryology and Andrology, Royan Institute, P O Box 19395 4644, Tehran, Iran
    J Assist Reprod Genet 25:197-203. 2008
    ....
  6. doi request reprint Artificial oocyte activation in severe teratozoospermia undergoing intracytoplasmic sperm injection
    Mohammad H Nasr-Esfahani
    Department of Embryology and Andrology, Royan Institute, Tehran, Iran
    Fertil Steril 90:2231-7. 2008
    ..To evaluate efficiency of ionomycin on fertilization and cleavage rates, embryo development, and pregnancy rate after intracytoplasmic sperm injection (ICSI) in teratozoospermic patients...
  7. ncbi request reprint Failed fertilization after ICSI and spermiogenic defects
    Mohammad H Nasr-Esfahani
    Department of Embryology and Andrology, Royan Institute, Tehran, Iran
    Fertil Steril 89:892-8. 2008
    ..To evaluate the relationship between late spermiogenic events, including histone-protamine replacement, acrosome integrity, and sperm morphology, with fertilization rate after intracytoplasmic sperm injection (ICSI)...
  8. ncbi request reprint Effects of failed oocyte activation and sperm protamine deficiency on fertilization post-ICSI
    Mohammad Hossein Nasr-Esfahani
    Department of Embryology and Andrology, Royan Institute, Tehran, Iran
    Reprod Biomed Online 14:422-9. 2007
    ..Furthermore, the results of this study did not show any correlation between pronuclei size asynchrony and protamine deficiency...
  9. ncbi request reprint Can sperm protamine deficiency induce sperm premature chromosomal condensation?
    M H Nasr-Esfahani
    Department of Embryology, Royan Institute, Tehran, Iran
    Andrologia 38:92-8. 2006
    ..46, P = 0.004). Therefore, it can be suggested that oocytes injected with protamine deficient sperm have a higher chance of forming PCC sperm and may result in failed fertilization post-ICSI...
  10. ncbi request reprint Varicocelectomy: semen parameters and protamine deficiency
    M H Nasr-Esfahani
    Department of Embryology and Andrology, Royan Institute, Tehran, Iran
    Int J Androl 32:115-22. 2009
    ..Thus, it can be suggested that patients with low initial sperm count may benefit more from assisted reproductive techniques or varicocelectomy followed by assisted reproduction...
  11. doi request reprint Dual effects of peroxisome proliferator-activated receptor γ on embryonic stem cell self-renewal in presence and absence of leukemia inhibitory factor
    Maryam Peymani
    Department of Cellular Biotechnology at Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
    Eur J Cell Biol 92:160-8. 2013
    ..These results suggest that the pivotal role of PPARγ on mESC self-renewal depends on the presence and absence of LIF...
  12. doi request reprint Differentiation of human ES cell-derived neural progenitors to neuronal cells with regional specific identity by co-culturing of notochord and somite
    Hossein Salehi
    Department of Cell and Molecular Biology, Cell Science Research Center, Royan Institute for Animal Biotechnology, Esfahan, Iran
    Stem Cell Res 8:120-33. 2012
    ..These results indicate a very simple and efficient protocol to mimic in vivo events of human neural development in vitro which is important in the context of developmental neuroscience and cellular replacement therapies...
  13. doi request reprint Neuronal induction and regional identity by co-culture of adherent human embryonic stem cells with chicken notochords and somites
    Hossein Salehi
    Department of Cell and Molecular Biology, Royan Institute for Animal Biotechnology, Esfahan, Iran
    Int J Dev Biol 55:321-6. 2011
    ..This study provides a model through which in vivo neuronal induction events may be imitated...
  14. ncbi request reprint Effect of sperm DNA damage and sperm protamine deficiency on fertilization and embryo development post-ICSI
    Mohammad Hossein Nasr-Esfahani
    Department of Clinical and Experimental Embryology and Andrology, Royan Institute, Tehran, Iran
    Reprod Biomed Online 11:198-205. 2005
    ..Unlike protamine deficiency, sperm DNA fragmentation does not preclude fertilization. Nonetheless, embryos derived from spermatozoa with high DNA damage have a lower potential to reach blastocyst stage...
  15. doi request reprint Cotransplantation of human embryonic stem cell-derived neural progenitors and schwann cells in a rat spinal cord contusion injury model elicits a distinct neurogenesis and functional recovery
    Ali Niapour
    Department of Cell and Molecular Biology, Cell Science Research Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran
    Cell Transplant 21:827-43. 2012
    ..These findings have demonstrated that the cotransplantation of SCs with hESC-NPs could offer a synergistic effect, promoting neuronal differentiation and functional recovery...
  16. doi request reprint Transplantation of undifferentiated and induced human exfoliated deciduous teeth-derived stem cells promote functional recovery of rat spinal cord contusion injury model
    Zahra Taghipour
    Department of Cell and Molecular Biology, Cell Science Research Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran
    Stem Cells Dev 21:1794-802. 2012
    ..These findings have demonstrated that transplantation of SHED or its derivatives could be a suitable candidate for the treatment of SCI as well as other neuronal degenerative diseases...
  17. doi request reprint Differentiation of human embryonic stem cell-derived retinal progenitors into retinal cells by Sonic hedgehog and/or retinal pigmented epithelium and transplantation into the subretinal space of sodium iodate-injected rabbits
    Noushin Amirpour
    Department of Cell and Molecular Biology, Royan Institute for Animal Biotechnology, Isfahan, Iran
    Stem Cells Dev 21:42-53. 2012
    ..Our results indicate that putative hESC-derived retinal cells express related genes and proteins. Further, our results show that retinal-like cells can be useful replacements for photoreceptors in retinal diseases...
  18. doi request reprint Neural induction in mouse embryonic stem cells by co-culturing with chicken somites
    Mohsen Sagha
    Department of Stem Cells, Cell Science Research Center, Royan Institute, Esfahan Campus, Iranian Academic Center for Education, Culture, and Research ACECR, Esfahan, Iran
    Stem Cells Dev 18:1351-60. 2009
    ..However, no effective influence of somitic co-culture on rostrocaudal or dorsoventral patterning is seen. Therefore, like amphibians, somites have a modest neural-inducing activity in amniotes...
  19. doi request reprint Influence of sperm chromatin anomalies on assisted reproductive technology outcome
    Marzeyeh Tavalaee
    Department of Embryology and Andrology, Royan Institute, Tehran, Iran
    Fertil Steril 91:1119-26. 2009
    ..To evaluate the influence of DNA fragmentation, DNA methylation, and protamine deficiency as indicators of chromatin status on assisted reproductive technology outcome, and also to assess the relation between these parameters...
  20. doi request reprint Sperm DNA damage and its relation with leukocyte DNA damage
    Zahra Babazadeh
    Department of Anatomy, Isfahan Medical University, Isfahan, Iran
    Reprod Toxicol 29:120-4. 2010
    ..The results showed that leukocyte DNA integrity could be used to identify individuals at high risk in order to reduce the extent of DNA damage in patients before ICSI in order to improve the subsequent outcome of this procedure...
  21. doi request reprint Nurr1 and PPARγ protect PC12 cells against MPP(+) toxicity: involvement of selective genes, anti-inflammatory, ROS generation, and antimitochondrial impairment
    Mohammad Jodeiri Farshbaf
    Department of Biology, School of Sciences, University of Isfahan, Isfahan, 81746 73441, Iran
    Mol Cell Biochem 420:29-42. 2016
    ..Moreover, production of intracellular ROS and MMP modulated by MPP(+) was decreased by PPARγ and Nurr1 agonists' treatment alone and in the combined form. ..
  22. doi request reprint Contrasting effects of G1.2/G2.2 and SOF1/SOF2 embryo culture media on pre- and post-implantation development of non-transgenic and transgenic cloned goat embryos
    Sayed Morteza Hosseini
    Department of Reproduction and Development, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
    Reprod Biomed Online 31:372-83. 2015
    ..It was concluded that the long-term programming effects of embryo culture medium and/or embryo production method may irreversibly affect post-implantation development of cloned embryos through defined molecular pathways. ..
  23. doi request reprint Lentiviral vector-mediated transduction of goat undifferentiated spermatogonia
    Hassan Abbasi
    Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Iran
    Anim Reprod Sci 163:10-7. 2015
    ....
  24. pmc Fabrication, characterization and cellular compatibility of poly(hydroxy alkanoate) composite nanofibrous scaffolds for nerve tissue engineering
    Elahe Masaeli
    Department of Textile Engineering, Isfahan University of Technology, Isfahan, Iran
    PLoS ONE 8:e57157. 2013
    ....
  25. doi request reprint Fndc5 overexpression facilitated neural differentiation of mouse embryonic stem cells
    Mahboobeh Forouzanfar
    Department of Cellular Biotechnology at Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran Department of Biology, School of Sciences, University of Isfahan, Isfahan, Iran
    Cell Biol Int 39:629-37. 2015
    ..Our findings are consistent with recent studies reporting a similar role for Fndc5 in proliferation of neural cells and increase in the expression of neurotrophins like BDNF. ..
  26. pmc Expression Profile of Developmentally Important Genes in preand peri-Implantation Goat Embryos Produced In Vitro
    Pouria Hosseinnia
    Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
    Int J Fertil Steril 10:310-319. 2016
    ....
  27. doi request reprint Vitrification at Day3 stage appears not to affect the methylation status of H19/IGF2 differentially methylated region of in vitro produced human blastocysts
    Marzieh Derakhshan-Horeh
    Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
    Cryobiology 73:168-74. 2016
    ....
  28. doi request reprint Enhanced expression of FNDC5 in human embryonic stem cell-derived neural cells along with relevant embryonic neural tissues
    Fatemeh Ahmadi Ghahrizjani
    Biology Department, Science and Research Branch, Islamic Azad University, Tehran, Iran
    Gene 557:123-9. 2015
    ..Additional research is necessary to determine the major function of FDNC5 in this process. ..
  29. doi request reprint The influence of dexamethasone administration on the protection against doxorubicin-induced cardiotoxicity in purified embryonic stem cell-derived cardiomyocytes
    Leila Dehghani
    Department of Cell and Molecular Biology, Cell Science Research Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran
    Tissue Cell 45:101-6. 2013
    ....
  30. doi request reprint Identification of a novel missense mutation of PEX7 gene in an Iranian patient with rhizomelic chondrodysplasia punctata type 1
    Parisa Mohamadynejad
    Biology Department, Science and Research Branch, Islamic Azad University, Tehran, Iran
    Gene 518:461-6. 2013
    ....
  31. pmc Characterization and Functional Assessment of Mouse PPARγ1 Promoter
    Liana Lachinani
    Department of Cell and Molecular Biology, Cell Science Research Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran
    Avicenna J Med Biotechnol 4:160-9. 2012
    ..During neural differentiation, PPARγ1 isoform expression reaches to maximal level at neural precursor cell formation...
  32. doi request reprint The influence of peroxisome proliferator-activated receptor γ(1) during differentiation of mouse embryonic stem cells to neural cells
    Ali Ghoochani
    Department of Cell and Molecular Biology, Cell Science Research Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran
    Differentiation 83:60-7. 2012
    ..Here, we have demonstrated the stage dependent role of PPARγ modulation on neural differentiation of mESCs by retinoic acid treatment for the first time...
  33. doi request reprint Novel method to obtain highly enriched cultures of adult rat Schwann cells
    Ali Niapour
    Department of Cell and Molecular Biology, Royan Institute for Animal Biotechnology, ACECR, P O Box 815896 8433, Isfahan, Iran
    Biotechnol Lett 32:781-6. 2010
    ..This method has the advantage of being technically easier, faster, and more efficient than other previously described methods. An SC culture that was about 99% homogenous was achieved...
  34. pmc Rational Development of A Polycistronic Plasmid with A CpG-Free Bacterial Backbone as A Potential Tool for Direct Reprogramming
    Kianoush Dormiani
    Department of Pharmaceutical Biotechnology and Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran Department of Molecular Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
    Cell J 18:565-581. 2017
    ..Accordingly, we designed and developed a small, non-integrating plasmid named pLENSO/Zeo as a 2A-mediated polycistronic expression vector...
  35. pmc Stage-Specific Profiling of Transforming Growth Factor-β, Fibroblast Growth Factor and Wingless-int Signaling Pathways during Early Embryo Development in The Goat
    Pouria Hosseinnia
    Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
    Cell J 17:648-58. 2016
    ....
  36. doi request reprint Cardiac differentiation of mouse embryonic stem cells is influenced by a PPAR γ/PGC-1α-FNDC5 pathway during the stage of cardiac precursor cell formation
    Faezeh Ghazvini Zadegan
    Department of Medical Genetic, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences Treatment and Health Services of Yazd, Yazd, Iran Department of Cellular Biotechnology at Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
    Eur J Cell Biol 94:257-66. 2015
    ..We concluded that PPARγ agonist and antagonist induced up and down-regulation of PGC-1α and subsequently modulated the process of CPCs formation through an alteration in FNDC5 and mitochondrial markers expression. ..
  37. pmc Epigenetic modification with trichostatin A does not correct specific errors of somatic cell nuclear transfer at the transcriptomic level; highlighting the non-random nature of oocyte-mediated reprogramming errors
    Sayyed Morteza Hosseini
    Department of Reproduction and Development, Reproductive Biomedicine Centre, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
    BMC Genomics 17:16. 2016
    ....
  38. doi request reprint Induced expression of Fndc5 significantly increased cardiomyocyte differentiation rate of mouse embryonic stem cells
    Farzaneh Rabiee
    Department of Biology, School of Sciences, University of Isfahan, Isfahan, Iran Department of Cellular Biotechnology at Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
    Gene 551:127-37. 2014
    ..These data suggest that Fndc5 may be involved in cardiomyocyte differentiation. Therefore, a new hope will be arisen for potential application of this myokine for regeneration of damaged cardiac tissues especially in cardiac failure. ..
  39. doi request reprint In vivo integration of poly(ε-caprolactone)/gelatin nanofibrous nerve guide seeded with teeth derived stem cells for peripheral nerve regeneration
    Mohammad Hossein Beigi
    Department of Cellular Biotechnology at Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran Materials Engineering Department, Najafabad Branch, Islamic Azad University, Najafabad, Iran
    J Biomed Mater Res A 102:4554-67. 2014
    ....
  40. doi request reprint Cytosolic and mitochondrial ROS: which one is associated with poor chromatin remodeling?
    Abbas Kiani-Esfahani
    Department of Reproductive Biotechnology at Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
    Syst Biol Reprod Med 59:352-9. 2013
    ..Rather, it is likely that apoptosis is induced by ROS production. Considering that these conclusions are derived from correlative analyses, additional studies including an interventive approach are required. ..
  41. doi request reprint Identification, cloning, and functional analysis of the TATA-less mouse FNDC5 promoter during neural differentiation
    Tahere Seifi
    Department of Biology, School of Sciences, University of Isfahan, Isfahan, Iran
    Cell Mol Neurobiol 34:715-25. 2014
    ..However, Noggin induction induced up regulation of FNDC5 expression at final stage of neural differentiation. In conclusion, stage dependent expression of FNDC5 is affected by neural induction method used for neural differentiation...
  42. ncbi request reprint C86Y: as a destructive homozygous mutation deteriorating Pex7p function causing rhizomelic chondrodysplasia punctata type I
    Ahmad Salamian
    Department of Cell and Molecular Biology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
    Ann Clin Lab Sci 43:76-80. 2013
    ..Back-transfection of vector encoding mutant Pex7p did not restore the normal peroxisomal function in RCDP patient's fibroblast cells dissimilar to the native type of PEX7...
  43. doi request reprint Induced in vitro differentiation of neural-like cells from human exfoliated deciduous teeth-derived stem cells
    Nosrat Nourbakhsh
    Pediatric Department, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran
    Int J Dev Biol 55:189-95. 2011
    ..SHED cells might be considered as new candidates for the autologous transplantation of a wide variety of neurological diseases and neurotraumatic injuries...
  44. ncbi request reprint The thickness of electrospun poly (epsilon-caprolactone) nanofibrous scaffolds influences cell proliferation
    Laleh Ghasemi-Mobarakeh
    Department of Stem Cells, Cell Science Research Center, Royan Institute, Esfahan Campus, ACECR, Esfahan Iran
    Int J Artif Organs 32:150-8. 2009
    ..Therefore, regardless of cell origin, thicker scaffolds provide a better substrate for cell proliferation, possibly due to the higher dimensional stability and tightness of thicker scaffolds...
  45. pmc Evaluation of the leptin receptor in human spermatozoa
    Leila Hatami-Baroogh
    Department of Reproduction and Development, Royan Institute for Animal Biotechnology, ACER, Isfahan, Iran
    Reprod Biol Endocrinol 8:17. 2010
    ..In this study, we have attempted to assess leptin receptor expression in the spermatozoa of fertile males and those diagnosed with male factor infertility; both at the mRNA or protein levels...
  46. pmc Evaluation of Fas positive sperm and complement mediated lysis in subfertile individuals
    Maryam Soleimani
    Department of Anatomy, Isfahan Medical University, Isfahan, Iran
    J Assist Reprod Genet 27:477-82. 2010
    ..Evaluation of Fas receptor on surface of sperm, as an apoptotic marker, using flow cytometry and confirming the results using an antibody-antigen complex through the classic complement pathway...
  47. doi request reprint Embryonic stem cell-derived cardiomyocytes as a model system to study cardioprotective effects of dexamethasone in doxorubicin cardiotoxicity
    Mahboubeh Farokhpour
    Department of Cell and Molecular Biology, Royan Institute for Animal Biotechnology, Esfahan, Iran
    Toxicol In Vitro 23:1422-8. 2009
    ..This model provides us with a clinical suggestion which proposes that the beneficial effect of DEX is obtained when DEX was added before DOX administration...
  48. pmc A Preliminary Study: N-acetyl-L-cysteine Improves Semen Quality following Varicocelectomy
    Foroogh Barekat
    Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran Department of Biology, Flavarjan Branch, Islamic Azad University, Flavarjan, Isfahan, Iran
    Int J Fertil Steril 10:120-6. 2016
    ..Therefore, the aim of this study is to evaluate the outcome of NAC on semen quality, protamine content, DNA damage, oxidative stress and fertility following varicocelectomy...
  49. doi request reprint Status of sperm-born oocyte activating factors (PAWP, PLCζ) and sperm chromatin in uncapacitated, capacitated and acrosome-reacted conditions
    Marziyeh Tavalaee
    a Department of Biology, School of Basic Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
    Hum Fertil (Camb) . 2017
    ..DGC processing, therefore, improved the quality of sperm in terms of chromatin and DNA integrity, and removed sperm which may have had a lower potential to induce oocyte activation and undergo capacitation and the acrosome reaction...
  50. pmc Diagnosis of genetic defects through parallel assessment of PLCζ and CAPZA3 in infertile men with history of failed oocyte activation
    Soudabeh Javadian-Elyaderani
    Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
    Iran J Basic Med Sci 19:281-9. 2016
    ..They contain a common bidirectional promoter. The objective of this study was to identify individuals with parallel low expression of PLCζ and CAPZA3 mRNA, in hope of detecting genetic defects in this bidirectional promoter...
  51. pmc Methylation Status of H19/IGF2 Differentially Methylated Region in in vitro Human Blastocysts Donated by Healthy Couples
    Marzieh Derakhshan-Horeh
    Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
    Iran Biomed J 21:16-23. 2017
    ..This study investigated the methylation status of H19/IGF2 DMR at high-quality expanding/expanded human blastocysts donated by healthy individuals to evaluate the risks linked to ART...
  52. pmc Assessment of DPY19L2 Deletion in Familial and Non-Familial Individuals with Globozoospermia and DPY19L2 Genotyping
    Parastoo Modarres
    Department of Cellular Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
    Int J Fertil Steril 10:196-207. 2016
    ..The aim of this study was to analyze the DPY19L2 gene deletion using polymerase chain reaction technique for the exons 1, 48, 11 and 22 as well as break point (BP) "a" in globozoospermic men...
  53. pmc Overexpression of Annexin A1 Suppresses Pro-Inflammatory Factors in PC12 Cells Induced by 1-Methyl-4-Phenylpyridinium
    Abbas Kiani-Esfahani
    Department of Cellular Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
    Cell J 18:197-204. 2016
    ..In this study, we therefore examined the potency of ANXA1 in regulating reactive oxygen species (ROS) production and suppressing pro-inflammatory responses in PC12 cells induced by 1-methyl-4-phenylpyridinium (MPP+)...
  54. pmc The Principal Forces of Oocyte Polarity Are Evolutionary Conserved but May Not Affect the Contribution of the First Two Blastomeres to the Blastocyst Development in Mammals
    Sayyed Morteza Hosseini
    Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
    PLoS ONE 11:e0148382. 2016
    ..Even though, the principal forces of this definitive oocyte polarity may not persist during embryonic cleavages. ..
  55. pmc Comparing Three Methods of Co-culture of Retinal Pigment Epithelium with Progenitor Cells Derived Human Embryonic Stem Cells
    Noushin Amirpour
    Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
    Int J Prev Med 4:1243-50. 2013
    ..It seems the effects of RPE cells on differentiation of retinal progenitor cells (RPCs) through the cell-to-cell contact or with the use of insert and compare of these methods has not been reported yet...
  56. ncbi request reprint Zinc finger protein 521 overexpression increased transcript levels of Fndc5 in mouse embryonic stem cells
    Motahere Sadat Hashemi
    Department of Cellular Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
    J Biosci 41:69-76. 2016
    ..Therefore it is concluding that an enhanced expression of Fndc5 in neural progenitor cells is stimulated by Zfp521 overexpression in these cells...
  57. doi request reprint Construction of a new minicircle DNA carrying an enhanced green florescent protein reporter gene for efficient expression into mammalian cell lines
    Nafiseh Sanei Ata-Abadi
    Department of Biology, School of Sciences, University of Isfahan, Isfahan, Iran
    Mol Biol Rep 42:1175-85. 2015
    ..Our constructed parental plasmid expresses EGFP and could be used for the generation of episomal minicircle DNA with intent to carry out transient transfection of interested DNA fragments into the eukaryotic cells for various purposes. ..
  58. doi request reprint A ground state of PPARγ activity and expression is required for appropriate neural differentiation of hESCs
    Marjan Taheri
    Biology Department, Science and Research Branch, Islamic Azad University, Tehran, Iran
    Pharmacol Rep 67:1103-14. 2015
    ..This study was conducted to testify the prominence of PPARγ during neural differentiation of human embryonic stem cells (hESCs)...
  59. doi request reprint Stem cell therapy in amyotrophic lateral sclerosis
    Rokhsareh Meamar
    Department of Medical Science, Najafabad Branch, Islamic Azad University, Isfahan, Iran
    J Clin Neurosci 20:1659-63. 2013
    ..We present a brief review of different methods of stem cell therapy in ALS patients and discuss the results with different cell types and routes of administration...
  60. doi request reprint THY1 as a reliable marker for enrichment of undifferentiated spermatogonia in the goat
    Hassan Abbasi
    Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Iran Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
    Theriogenology 80:923-32. 2013
    ..In conclusion, the results provide indications that THY1 surface marker can be reliably used for enrichment of undifferentiated spermatogonial in the goats. ..
  61. pmc Sperm MTT viability assay: a new method for evaluation of human sperm viability
    Mohammad Hossein Nasr-Esfahani
    Department of Clinical and Experimental Embryology and Andrology, Royan Institute, Tehran, Iran
    J Assist Reprod Genet 19:477-82. 2002
    ..The objective of this study was to evaluate the capability of MTT assay to discriminate between viable and nonviable sperms and compare it sefficiency with E&N (eosin and nigrosin) and HOST (hypo-osmotic swelling test)...
  62. doi request reprint Application of conductive polymers, scaffolds and electrical stimulation for nerve tissue engineering
    Laleh Ghasemi-Mobarakeh
    Islamic Azad University, Najafabad Branch, Isfahan, Iran
    J Tissue Eng Regen Med 5:e17-35. 2011
    ..Different procedures of electrical stimulation which have been used in tissue engineering, with examples on their specific applications in tissue engineering, are also discussed...
  63. ncbi request reprint Effect of human sperm chromatin anomalies on fertilization outcome post-ICSI
    S Razavi
    Department of Anatomy, Isfahan University of Medical Sciences, Isfahan, Iran
    Andrologia 35:238-43. 2003
    ..In conclusion, that sperm protamine deficiency has profound effect on fertilization failure in ICSI...
  64. ncbi request reprint Effect of protamine-2 deficiency on ICSI outcome
    M H Nasr-Esfahani
    Royan Institute, Tehran, Iran
    Reprod Biomed Online 9:652-8. 2004
    ..Therefore, it can be concluded that altered P1/P2 ratio effects fertilization rate and embryo quality which subsequently may affect implantation and pregnancy outcome...
  65. doi request reprint Selection of sperm based on combined density gradient and Zeta method may improve ICSI outcome
    M Kheirollahi-Kouhestani
    Department of Biology, Isfahan University, Isfahan, Iran
    Hum Reprod 24:2409-16. 2009
    ..The aim of this study was to evaluate the efficiency of density gradient centrifugation (DGC) and Zeta methods to recover sperm with intact chromatin, and to evaluate whether the combined DGC/Zeta procedure improved ICSI outcome...
  66. pmc PPARγ ameliorated LPS induced inflammation of HEK cell line expressing both human Toll-like receptor 4 (TLR4) and MD2
    Reyhaneh Darehgazani
    Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Velenjak, 1985717443, Tehran, Iran
    Cytotechnology 68:1337-48. 2016
    ..PPARγ was shown to negatively regulate TLR4 activity and therefore exerts its anti-inflammatory action against LPS induced inflammation. ..
  67. pmc Stem cells from human exfoliated deciduous tooth exhibit stromal-derived inducing activity and lead to generation of neural crest cells from human embryonic stem cells
    Khadijeh Karbalaie
    Department of Cellular Biotechnology at Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
    Cell J 17:37-48. 2015
    ..Due to the ethical and technical problems surrounding the isolation of these early human embryo cells, researchers have focused on in vitro studies to produce NCCs and increase their knowledge of neural crest development...
  68. doi request reprint Super magnetic nanoparticles NiFe2O4, coated with aluminum-nickel oxide sol-gel lattices to safe, sensitive and selective purification of his-tagged proteins
    Seyede Zohreh Mirahmadi-Zare
    Department of Molecular Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran Electronic address
    Protein Expr Purif 121:52-60. 2016
    ..The results confirmed that the synthesized nickel magnetic nanoparticles did not show metal ion toxicity and not affected on protein folding. ..
  69. doi request reprint Acute course of deferoxamine promoted neuronal differentiation of neural progenitor cells through suppression of Wnt/β-catenin pathway: a novel efficient protocol for neuronal differentiation
    Amin Ziaei
    Department of Cellular Biotechnology at Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
    Neurosci Lett 590:138-44. 2015
    ..We concluded that suppression of Wnt/β-catenin pathway was induced through chelating of intracellular Iron due to DFO treatment. These findings help to understand therapeutic benefit of DFO as a neuroprotective agent...
  70. doi request reprint Evaluation of heat-shock protein A2 (HSPA2) in male rats before and after varicocele induction
    Afsoon Atrian Afiyani
    Department of Reproductive Biotechnology at Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran Department of Biochemistry, Science and Research Branch, Islamic Azad University, Kurdistan, Iran
    Mol Reprod Dev 81:766-76. 2014
    ..Levels of ubiquitination were also remarkably lower in the left testis of the varicocele group. Therefore, varicocele impacts expression of HSPA2 and ubiquitination...
  71. pmc Unexpected detrimental effect of Insulin like growth factor-1 on bovine oocyte developmental competence under heat stress
    Mahdi Zhandi
    Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, POBox 4111, Karaj, Iran
    J Assist Reprod Genet 26:605-11. 2009
    ..To investigate the effects of Insulin like growth factor-1 (IGF-1) on bovine oocyte developmental competence under heat stress...
  72. doi request reprint Generation of motor neurons by coculture of retinoic acid-pretreated embryonic stem cells with chicken notochords
    Maryam Anjomshoa
    Department of Stem Cells, Cell Science Research Center, Royan Institute, ACECR, Esfahan Campus, Esfahan, Iran
    Stem Cells Dev 18:259-67. 2009
    ....
  73. doi request reprint Toxicity of ecstasy (MDMA) towards embryonic stem cell-derived cardiac and neural cells
    Rokhsareh Meamar
    Department of Cell and Molecular Biology, Royan Institute for Animal Biotechnology, P O Box 815896 8433, Esfahan, Iran
    Toxicol In Vitro 24:1133-8. 2010
    ..In conclusion, MDMA causes a marked reduction in beating cardiomyocytes and neurons in ESC cultures, and this drug has a more potent toxicity on neural rather than cardiac cell differentiation...
  74. doi request reprint Expression profile of FGF receptors in preimplantation ovine embryos and the effect of FGF2 and PD173074
    Mehdi Moradi
    a Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
    Growth Factors 33:393-400. 2015
    ..In summary, FGF2-mediated activation of FGFRs may promote a switch in transcriptional profile of ovine ICM from EPI- to hypoblast-associated gene expression. ..
  75. doi request reprint Does PGC1α/FNDC5/BDNF Elicit the Beneficial Effects of Exercise on Neurodegenerative Disorders?
    Mohammad Jodeiri Farshbaf
    Department of Cellular Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Royan Street, Salman Street, Khorasgan, Isfahan, 8165131378, Iran
    Neuromolecular Med 18:1-15. 2016
    ..This review presents recent findings on the PGC1α/FNDC5/BDNF pathway and its role in neuroprotection, and discusses the controversial promise of irisin as a mediator of the positive benefits of exercise. ..
  76. pmc miR-141 and miR-200a, Revelation of New Possible Players in Modulation of Th17/Treg Differentiation and Pathogenesis of Multiple Sclerosis
    Reza Naghavian
    Division of Cellular and Molecular Biology, Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran Department of Cellular Biotechnology at Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
    PLoS ONE 10:e0124555. 2015
    ..There are growing interests in nominating miRNAs involved in Th17 cell differentiation, suggesting them as new therapeutic agents that may reduce progression of different autoimmune diseases specially MS...
  77. doi request reprint Addition of Tempol in semen cryopreservation medium improves the post-thaw sperm function
    Zahra Bateni
    Department of Reproductive Biotechnology at Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
    Syst Biol Reprod Med 60:245-50. 2014
    ..These effects of Tempol can be attributed to cell permeability characteristic and ability to reduce superoxide production both at intra- and extra-cellular levels. Tempol may hold the potential for clinical applications...
  78. pmc Could MDMA Promote Stemness Characteristics in Mouse Embryonic Stem Cells via mGlu5 Metabotropic Glutamate Receptors?
    Rokhsareh Meamar
    1 Department of Cell and Molecular Biology, Cell Science Research Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran 2 Isfahan Neurosciences research center, Isfahan University of Medical Sciences, Isfahan, Iran 3 Islamic Azad University, Najafabad Branch, Isfahan, Iran
    Cell J 14:185-92. 2012
    ..This study aims to investigate whether MDMA could influence self-renewal via the mGlu5 receptor in mESCs...
  79. pmc Somatic cell-induced hyperacetylation, but not hypomethylation, positively and reversibly affects the efficiency of in vitro cloned blastocyst production in cattle
    Farnoosh Jafarpour
    Department of Reproduction and Development, Reproductive Biomedicine Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran
    Cell Reprogram 13:483-93. 2011
    ..Moreover, H3K9 hyperacetylation (induced with TSA and NaB), but not DNA hypomethylation (induced with AzC), favors cloning efficiency...
  80. ncbi request reprint Can Vero cell co-culture improve in-vitro maturation of bovine oocytes?
    Fariba Moulavi
    Royan Institute, Tehran, Iran
    Reprod Biomed Online 13:404-11. 2006
    ..5, 245.5 and 51.3, 265, 316.3 respectively). Results confirm that co-culture of bovine COC during in-vitro maturation, enhances their ability for cleavage and for producing blastocysts with higher quality...
  81. doi request reprint Cytoplasmic, rather than nuclear-DNA, insufficiencies as the major cause of poor competence of vitrified oocytes
    Sayyed Morteza Hosseini
    Department of Reproduction and Development, Reproductive Biomedicine Center, Royan Institute for Biotechnology, ACECR, P O Box 81593 58686, Isfahan, Iran Electronic address
    Reprod Biomed Online 30:549-52. 2015
    ..05). These results point toward 'cytoplasmic insufficiencies' as the main cause of poor 'competence' of matured oocytes after vitrification. ..
  82. doi request reprint Hedgehog signalling is dispensable in the proliferation of stem cells from human exfoliated deciduous teeth
    Fatemeh Ejeian
    Department of Cellular Biotechnology at Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
    Cell Biol Int 38:480-7. 2014
    ..Our findings have suggested the possibility that SHEDs demonstrate a different potential from human bone marrow mesenchymal stem cells (h-BMSCs) and dorsal neural progenitor in response to growth factors such as SHH. ..
  83. doi request reprint Peroxisome proliferator activated receptor gamma (PPARγ) as a therapeutic target for improvement of cognitive performance in Fragile-X
    Mohammad Jodeiri Farshbaf
    Department of Biology, School of Sciences, University of Isfahan, Isfahan, Iran Department of Cellular Biotechnology at Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
    Med Hypotheses 82:291-4. 2014
    ..The present hypothesis consists of enhancing synaptic plasticity that may then rescue the learning and memory in FXS. This will open many new therapeutic avenues for a variety of human diseases. ..
  84. ncbi request reprint Relation between different human sperm nuclear maturity tests and in vitro fertilization
    M H Nasr-Esfahani
    Isfahan Fertility and Infertility Center, Isfahan, Iran
    J Assist Reprod Genet 18:219-25. 2001
    ..To consider the relationship between different sperm nuclear maturity tests and in vitro fertilization (IVF) rate, in order to select the most sensitive, specific, and independent factor(s) for prediction of in vitro fertilization...