Mike Heilemann

Summary

Publications

  1. doi Subdiffraction-resolution fluorescence imaging with conventional fluorescent probes
    Mike Heilemann
    Angewandte Laserphysik and Laserspektroskopie, Universitat Bielefeld, Universitatsstrasse 25, 33615 Bielefeld, Germany
    Angew Chem Int Ed Engl 47:6172-6. 2008
  2. doi Subdiffraction fluorescence imaging of biomolecular structure and distributions with quantum dots
    Meike Heidbreder
    Physics Department, Bielefeld University, Universitatsstrasse 25, 33615 Bielefeld, Germany
    Biochim Biophys Acta 1803:1224-9. 2010
  3. doi Subdiffraction-resolution fluorescence microscopy of myosin-actin motility
    Ulrike Endesfelder
    Applied Laser Physics and Laser Spectroscopy, Physics Department, Bielefeld University, Universitatsstrasse 25, 33615 Bielefeld, Germany
    Chemphyschem 11:836-40. 2010
  4. doi Multicolor photoswitching microscopy for subdiffraction-resolution fluorescence imaging
    Sebastian van de Linde
    Bielefeld Institute for Biophysics and Nanoscience, Bielefeld University, Universitatsstrasse 25, 33615, Bielefeld, Germany
    Photochem Photobiol Sci 8:465-9. 2009
  5. ncbi Dissecting and reducing the heterogeneity of excited-state energy transport in DNA-based photonic wires
    Mike Heilemann
    Applied Laser Physics and Laser Spectroscopy, Bielefeld University, Universitatsstrasse 25, 33615 Bielefeld, Germany
    J Am Chem Soc 128:16864-75. 2006
  6. doi Fluorescent proteins for single-molecule fluorescence applications
    Britta Seefeldt
    Applied Laser Physics and Laser Spectroscopy, University of Bielefeld, Physics Department, Universitatsstrasse 25, 33615 Bielefeld, Germany
    J Biophotonics 1:74-82. 2008
  7. doi Spiropyrans as molecular optical switches
    Britta Seefeldt
    Applied Laser Physics and Laser Spectroscopy and Bielefeld Institute for Biophysics and Nanoscience, Bielefeld University, Universitatsstrasse 25, 33615, Bielefeld, Germany
    Photochem Photobiol Sci 9:213-20. 2010
  8. doi Subdiffraction-resolution fluorescence imaging of proteins in the mitochondrial inner membrane with photoswitchable fluorophores
    Sebastian van de Linde
    Applied Laser Physics and Laser Spectroscopy, Bielefeld University, Universitatsstrasse 25, 33615 Bielefeld, Germany
    J Struct Biol 164:250-4. 2008
  9. doi Super-resolution imaging with small organic fluorophores
    Mike Heilemann
    Applied Laser Physics and Laser Spectroscopy, Bielefeld University, Universitatsstrasse 25, 33615 Bielefeld, Germany
    Angew Chem Int Ed Engl 48:6903-8. 2009
  10. ncbi Carbocyanine dyes as efficient reversible single-molecule optical switch
    Mike Heilemann
    Applied Laserphysics and Laserspectroscopy, Physics Faculty, University of Bielefeld, Universitatsstrasse 25, 33615 Bielefeld, Germany
    J Am Chem Soc 127:3801-6. 2005

Collaborators

  • Markus Sauer
  • Philip Tinnefeld
  • Britta Seefeldt
  • Thomas Huser
  • Thorsten Seidel
  • Karl Josef Dietz
  • Sebastian van de Linde
  • Ulrike Endesfelder
  • Simon Hennig
  • Robin Diekmann
  • Mark Schüttpelz
  • Christoph T Nottbohm
  • Meike Heidbreder
  • Steve Wolter
  • Christian M Roth
  • Dirk Peter Herten
  • Thomas Heinlein
  • Deanna L Wolfson
  • Christoph Spahn
  • Barbara Kaltschmidt
  • Ran Sopher
  • Christian Kaltschmidt
  • Armin Gölzhäuser
  • Darius Widera
  • Anindita Mukherjee
  • Gerd Wiebusch
  • Pia I Heinlein
  • Christian Muller
  • Pia Schlüter
  • Jürgen Wolfrum
  • Christian Michael Roth
  • Andreas Biebricher

Detail Information

Publications16

  1. doi Subdiffraction-resolution fluorescence imaging with conventional fluorescent probes
    Mike Heilemann
    Angewandte Laserphysik and Laserspektroskopie, Universitat Bielefeld, Universitatsstrasse 25, 33615 Bielefeld, Germany
    Angew Chem Int Ed Engl 47:6172-6. 2008
  2. doi Subdiffraction fluorescence imaging of biomolecular structure and distributions with quantum dots
    Meike Heidbreder
    Physics Department, Bielefeld University, Universitatsstrasse 25, 33615 Bielefeld, Germany
    Biochim Biophys Acta 1803:1224-9. 2010
    ....
  3. doi Subdiffraction-resolution fluorescence microscopy of myosin-actin motility
    Ulrike Endesfelder
    Applied Laser Physics and Laser Spectroscopy, Physics Department, Bielefeld University, Universitatsstrasse 25, 33615 Bielefeld, Germany
    Chemphyschem 11:836-40. 2010
    ..We measured velocities of individual actin filaments of up to approximately 0.18 microm s(-1), observed strong bending and disruption of filaments as well as locally immobile fragments...
  4. doi Multicolor photoswitching microscopy for subdiffraction-resolution fluorescence imaging
    Sebastian van de Linde
    Bielefeld Institute for Biophysics and Nanoscience, Bielefeld University, Universitatsstrasse 25, 33615, Bielefeld, Germany
    Photochem Photobiol Sci 8:465-9. 2009
    ....
  5. ncbi Dissecting and reducing the heterogeneity of excited-state energy transport in DNA-based photonic wires
    Mike Heilemann
    Applied Laser Physics and Laser Spectroscopy, Bielefeld University, Universitatsstrasse 25, 33615 Bielefeld, Germany
    J Am Chem Soc 128:16864-75. 2006
    ..Finally, we used multiparameter SMFS to analyze the energy flow in photonic wires in more detail and to assign residual heterogeneity under optimized conditions in solution to different leakages and competing energy-transfer processes...
  6. doi Fluorescent proteins for single-molecule fluorescence applications
    Britta Seefeldt
    Applied Laser Physics and Laser Spectroscopy, University of Bielefeld, Physics Department, Universitatsstrasse 25, 33615 Bielefeld, Germany
    J Biophotonics 1:74-82. 2008
    ....
  7. doi Spiropyrans as molecular optical switches
    Britta Seefeldt
    Applied Laser Physics and Laser Spectroscopy and Bielefeld Institute for Biophysics and Nanoscience, Bielefeld University, Universitatsstrasse 25, 33615, Bielefeld, Germany
    Photochem Photobiol Sci 9:213-20. 2010
    ..e. photoswitching of the photochromic acceptor. Furthermore, we present a water soluble switchable spiropyran derivative and demonstrate the first intermolecular single-molecule photoswitching experiments in polymers...
  8. doi Subdiffraction-resolution fluorescence imaging of proteins in the mitochondrial inner membrane with photoswitchable fluorophores
    Sebastian van de Linde
    Applied Laser Physics and Laser Spectroscopy, Bielefeld University, Universitatsstrasse 25, 33615 Bielefeld, Germany
    J Struct Biol 164:250-4. 2008
    ..Besides the high localization precision of individual proteins we demonstrate how quantitative data, i.e. the protein distribution in the membrane, can be derived and compared...
  9. doi Super-resolution imaging with small organic fluorophores
    Mike Heilemann
    Applied Laser Physics and Laser Spectroscopy, Bielefeld University, Universitatsstrasse 25, 33615 Bielefeld, Germany
    Angew Chem Int Ed Engl 48:6903-8. 2009
  10. ncbi Carbocyanine dyes as efficient reversible single-molecule optical switch
    Mike Heilemann
    Applied Laserphysics and Laserspectroscopy, Physics Faculty, University of Bielefeld, Universitatsstrasse 25, 33615 Bielefeld, Germany
    J Am Chem Soc 127:3801-6. 2005
    ....
  11. ncbi Quantum dot triexciton imaging with three-dimensional subdiffraction resolution
    Simon Hennig
    Applied Laser Physics and Laser Spectroscopy and Bielefeld Institute for Biophysics and Nanoscience, Bielefeld University, Universitatsstrasse 25, 33615 Bielefeld, Germany
    Nano Lett 9:2466-70. 2009
    ..As a pure physical approach, the resolution enhancement is independent from the nanoenvironment and demonstrated to work in living cells...
  12. pmc Nanoscopy of bacterial cells immobilized by holographic optical tweezers
    Robin Diekmann
    Biomolecular Photonics, Department of Physics, University of Bielefeld, Universitatsstrasse 25, 33615 Bielefeld, Germany
    Nat Commun 7:13711. 2016
    ..This opens up new opportunities to super-resolve the nanoscale organization of chromosomal DNA in individual bacterial cells...
  13. doi Fluorescently labeled 1 nm thin nanomembranes
    Christoph T Nottbohm
    Fakultat fur Physik, Universitat Bielefeld, Universitatsstr 25, 33615 Bielefeld, Germany
    J Biotechnol 149:267-71. 2010
    ..Fluorescently labeled freestanding nanosheets, i.e. nanomembranes were obtained by a similar transfer of the nanosheets to TEM grids...
  14. doi Fluorescence microscopy beyond the diffraction limit
    Mike Heilemann
    Applied Laser Physics and Laser Spectroscopy, Physics Department, Bielefeld University, Universitatsstrasse 25, 33615 Bielefeld, Germany
    J Biotechnol 149:243-51. 2010
    ..The underlying physical concepts are discussed, and practical considerations for the application of these methods are made...
  15. ncbi Imaging diffusion in living cells using time-correlated single-photon counting
    Christian M Roth
    Physikalisch Chemisches Insitut, Universitat Heidelberg, Im Neuenheimer Feld 253, 69120 Heidelberg, Germany
    Anal Chem 79:7340-5. 2007
    ..We further demonstrate the applicability of the method to living cells and recorded a diffusion map of a living 3T3 mouse fibroblast incubated with dT20-ATTO488...
  16. ncbi High-resolution colocalization of single molecules within the resolution gap of far-field microscopy
    Thomas Heinlein
    Institute of Physical Chemistry, University of Heidelberg, Im Neuenheimer Feld 253, 69120 Heidelberg, Germany
    Chemphyschem 6:949-55. 2005
    ....