Amita Gupta

Summary

Publications

  1. doi request reprint Extended-dose nevirapine to 6 weeks of age for infants to prevent HIV transmission via breastfeeding in Ethiopia, India, and Uganda: an analysis of three randomised controlled trials
    Abubaker Bedri
    Lancet 372:300-13. 2008
  2. pmc Directed evolution of an anti-human red blood cell antibody
    Amita Gupta
    Department of Biochemistry, University of Delhi South Campus, New Delhi, India
    MAbs 1:268-80. 2009
  3. pmc Bifunctional recombinant fusion proteins for rapid detection of antibodies to both HIV-1 and HIV-2 in whole blood
    Amita Gupta
    Department of Biochemistry, University of Delhi South Campus, Benito Juarez Road, New Delhi 110 021, India
    BMC Biotechnol 6:39. 2006
  4. ncbi request reprint Expression and purification of recombinant antigens of Mycobacterium tuberculosis for application in serodiagnosis
    Abhishek Kulshrestha
    Department of Biochemistry, University of Delhi South Campus, New Delhi 110 021, India
    Protein Expr Purif 44:75-85. 2005
  5. pmc Whole-blood agglutination assay for on-site detection of human immunodeficiency virus infection
    Amita Gupta
    Department of Biochemistry, University of Delhi South Campus, New Delhi 110 021, India
    J Clin Microbiol 41:2814-21. 2003
  6. pmc Rapid restriction enzyme-free cloning of PCR products: a high-throughput method applicable for library construction
    Vijay K Chaudhary
    Department of Biochemistry, University of Delhi South Campus, Benito Juarez Road, New Delhi 110021, India
    PLoS ONE 9:e111538. 2014
  7. doi request reprint A new microarray platform for whole-genome expression profiling of Mycobacterium tuberculosis
    Balaji Venkataraman
    Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi 110021, India
    J Microbiol Methods 97:34-43. 2014
  8. pmc A novel helper phage enabling construction of genome-scale ORF-enriched phage display libraries
    Amita Gupta
    Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi, India
    PLoS ONE 8:e75212. 2013
  9. doi request reprint Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis
    Amita Gupta
    Department of Microbiology, University of Delhi South Campus, New Delhi, India
    FEMS Microbiol Lett 290:45-53. 2009
  10. ncbi request reprint Expression, purification, and characterization of an anti-RBCFab-p24 fusion protein for hemagglutination-based rapid detection of antibodies to HIV in whole blood
    Amita Gupta
    Department of Biochemistry, University of Delhi South Campus, Benito Juarez Road, 110 021, New Delhi, India
    Protein Expr Purif 26:162-70. 2002

Collaborators

  • S K Sharma
  • Anil K Tyagi
  • Kuyilan Karai Subramanian
  • Masanori Onda
  • Vijay K Chaudhary
  • Shruti Mehta
  • Steven J Reynolds
  • K A Gebo
  • Mary Glenn Fowler
  • Susan Eshleman
  • Seema Sahay
  • JAY B JACKSON
  • Ira Pastan
  • Madhuri R Thakar
  • I Shah
  • Sanjay M Mehendale
  • David Celentano
  • L H Moulton
  • Robert C Bollinger
  • Balaji Venkataraman
  • Jayagowri Sastry
  • Nikhil Gupte
  • Harjot Kaur Singh
  • Abubaker Bedri
  • Uma Nayak
  • Chelsea B Polis
  • Suneil R Ramchandani
  • Ramona Sobhani
  • Catherine Passaretti
  • Gita Sinha
  • Bijal Shah
  • Abhishek Kulshrestha
  • Madavan Vasudevan
  • Nidhi Gupta
  • Clemensia Nakabiito
  • Raman Raghunathrao Gangakhedkar
  • Abdulhamid Isehak
  • Michael Mubiru
  • Arti Kinikar
  • Philippa Musoke
  • Srikanth Tripathy
  • Rahel Adamu
  • Lidia Propper
  • Elham Hassen
  • Nishi Suryavanshi
  • Saad B Omer
  • Yohannes Mengistu
  • Adrian Taylor
  • Sheryl Zwerski
  • Jacqueline Coberly
  • George Kelly Siberry
  • Arvind V Bhore
  • Carolyne Onyango
  • Solomon Kumbi
  • Vinay Kulkarni
  • Mohammad A Chaudhary
  • Patricia Bright
  • Berhanu Gudetta
  • Francis Mmiro
  • Andrea J Ruff
  • Gretchen Antelman
  • Aida Abashawl
  • Laura Guay
  • Sileshi Lulseged
  • Anita Shankar
  • Ramesh Bhosale
  • Robert Cyril Bollinger
  • Malathi Ram
  • Vivian Rexroad
  • Venkat Varadhrajan
  • Shashikala A Sangle
  • Anita Basavaraj
  • Sandeep Nair
  • Medha M Khandekar
  • Arjun L Kakrani
  • Tou J Choi
  • Ved P Pandey
  • Kapila E Bharucha
  • Kristine E Johnson
  • Anandini S Kshirsagar
  • Abhay Chowdhary
  • Satish B Vaidya
  • Joline Choi
  • Jeetender P Ramnani
  • Snehal N Shah
  • Ramesh A Bhosale
  • Haridas B Prasad
  • Shweta N Morde
  • Sikhamani Rajasekaran
  • R D Kharkar

Detail Information

Publications26

  1. doi request reprint Extended-dose nevirapine to 6 weeks of age for infants to prevent HIV transmission via breastfeeding in Ethiopia, India, and Uganda: an analysis of three randomised controlled trials
    Abubaker Bedri
    Lancet 372:300-13. 2008
    ..The aim of three trials in Ethiopia, India, and Uganda was to assess whether daily nevirapine given to breastfed infants through 6 weeks of age can decrease HIV transmission via breastfeeding...
  2. pmc Directed evolution of an anti-human red blood cell antibody
    Amita Gupta
    Department of Biochemistry, University of Delhi South Campus, New Delhi, India
    MAbs 1:268-80. 2009
    ..Structural modelling revealed that B6 Fab mutants had increased hydrogen bonding resulting in increased stability. Our approach provides a novel and useful strategy to obtain recombinant antibodies with improved characteristics...
  3. pmc Bifunctional recombinant fusion proteins for rapid detection of antibodies to both HIV-1 and HIV-2 in whole blood
    Amita Gupta
    Department of Biochemistry, University of Delhi South Campus, Benito Juarez Road, New Delhi 110 021, India
    BMC Biotechnol 6:39. 2006
    ..Since it is not required of a screening test to differentially detect HIV and HIV-2, it would useful to create a single molecule capable of simultaneous detection of both HIV-1 and HIV-2 in a drop of blood...
  4. ncbi request reprint Expression and purification of recombinant antigens of Mycobacterium tuberculosis for application in serodiagnosis
    Abhishek Kulshrestha
    Department of Biochemistry, University of Delhi South Campus, New Delhi 110 021, India
    Protein Expr Purif 44:75-85. 2005
    ..tuberculosis specific antibodies...
  5. pmc Whole-blood agglutination assay for on-site detection of human immunodeficiency virus infection
    Amita Gupta
    Department of Biochemistry, University of Delhi South Campus, New Delhi 110 021, India
    J Clin Microbiol 41:2814-21. 2003
    ..Because the test is extremely rapid, requires no sample preparation, and is simple enough to be performed by a semiskilled technician in any remote area, NEVA HIV is a test for the hard-to-reach populations of the world...
  6. pmc Rapid restriction enzyme-free cloning of PCR products: a high-throughput method applicable for library construction
    Vijay K Chaudhary
    Department of Biochemistry, University of Delhi South Campus, Benito Juarez Road, New Delhi 110021, India
    PLoS ONE 9:e111538. 2014
    ..Additionally, based on this strategy, a variety of vectors were constructed for the expression of proteins in E. coli, enabling large number of different clones to be rapidly generated. ..
  7. doi request reprint A new microarray platform for whole-genome expression profiling of Mycobacterium tuberculosis
    Balaji Venkataraman
    Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi 110021, India
    J Microbiol Methods 97:34-43. 2014
    ..Further, the quality of the generated data has been compared with published data and is superior to that obtained using spotted oligonucleotide microarrays. ..
  8. pmc A novel helper phage enabling construction of genome-scale ORF-enriched phage display libraries
    Amita Gupta
    Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi, India
    PLoS ONE 8:e75212. 2013
    ..The ORF-enriched libraries were successfully used for identification of linear and conformational epitopes for monoclonal antibodies specific to mycobacterial proteins. ..
  9. doi request reprint Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis
    Amita Gupta
    Department of Microbiology, University of Delhi South Campus, New Delhi, India
    FEMS Microbiol Lett 290:45-53. 2009
    ..These toxins need to be further tested for their activity in the native host and other organism backgrounds and growth environments for utilization of their antibacterial potential...
  10. ncbi request reprint Expression, purification, and characterization of an anti-RBCFab-p24 fusion protein for hemagglutination-based rapid detection of antibodies to HIV in whole blood
    Amita Gupta
    Department of Biochemistry, University of Delhi South Campus, Benito Juarez Road, 110 021, New Delhi, India
    Protein Expr Purif 26:162-70. 2002
    ....
  11. doi request reprint A robust and efficient method for the isolation of DNA-free, pure and intact RNA from Mycobacterium tuberculosis
    Balaji Venkataraman
    Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi 110021, India
    J Microbiol Methods 93:198-202. 2013
    ..The method reduces the use of hazardous chemicals and incorporates protocols for efficient removal of gDNA and rRNA...
  12. ncbi request reprint High-density functional display of proteins on bacteriophage lambda
    Amita Gupta
    Department of Biochemistry, University of Delhi South Campus, New Delhi 110021, India
    J Mol Biol 334:241-54. 2003
    ..The high-density display of foreign proteins on bacteriophage lambda should be extremely useful in studying low-affinity protein-protein interactions more efficiently compared to the M13 phage-based system...
  13. ncbi request reprint Expression and purification of recombinant 38-kDa and Mtb81 antigens of Mycobacterium tuberculosis for application in serodiagnosis
    Vijay K Chaudhary
    Department of Biochemistry, University of Delhi South Campus, New Delhi 110 021, India
    Protein Expr Purif 40:169-76. 2005
    ..The purified proteins were useful in detecting antibodies in sera of tuberculosis patients (smear positive, smear negative, and extra-pulmonary categories)...
  14. ncbi request reprint Selenium and HIV-1: hope or hype?
    Catherine Passaretti
    Arch Intern Med 167:2530-1. 2007
  15. ncbi request reprint GB virus type C: a virus in search of a disease or a role in HIV therapy?
    Robert C Bollinger
    Clin Infect Dis 38:410-1. 2004
  16. ncbi request reprint Limited evolution in the HIV type 1 pol region among acute seroconverters in Pune, India
    Susan H Eshleman
    The Johns Hopkins Medical Institutions, Baltimore, MD 21205, USA
    AIDS Res Hum Retroviruses 21:93-7. 2005
    ..Amino acid substitutions were rare, and varied from subject to subject. In this cohort, drug resistance was not observed and evolution in the pol region was very limited during the first 2 years of infection...
  17. ncbi request reprint The Indian pediatric HIV epidemic: a systematic review
    Harjot Kaur Singh
    Johns Hopkins University School of Medicine, Division of Infectious Diseases, Baltimore, MD 21287, USA
    Curr HIV Res 6:419-32. 2008
    ..Targeted interventions to curb India's pediatric HIV epidemic require urgent clarification of these findings. If such differences truly exist, management guidelines should be tailored accordingly...
  18. ncbi request reprint Same-sex behavior and high rates of HIV among men attending sexually transmitted infection clinics in Pune, India (1993-2002)
    Amita Gupta
    Division of Infectious Disease, Johns Hopkins School of Medicine, 600 North Wolfe Street, Baltimore, MD 21287, USA
    J Acquir Immune Defic Syndr 43:483-90. 2006
    ..To determine HIV/sexually transmitted infection (STI) prevalence, trends, and risk behaviors of men who have sex with men (MSM) and compare these with those of non-MSM attending STI clinics in Pune, India over a 10-year period...
  19. ncbi request reprint Mother-to-child transmission of HIV among women who chose not to exclusively breastfeed their infants in Pune, India
    Amita Gupta
    Division of Infectious Disease, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA
    Indian J Med Res 126:131-4. 2007
    ..We examined HIV MTCT rates among 41 Indian women in a prospective cohort who chose predominantly not to exclusively breastfeed...
  20. ncbi request reprint Mortality & clinical characteristics of hospitalized adult patients with HIV in Pune, India
    Ramona Sobhani
    Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Indian J Med Res 126:116-21. 2007
    ..The objective of our study was to determine mortality and clinical characteristics of hospitalized HIV-infected individuals in a high HIV prevalence region of India...
  21. ncbi request reprint Low sensitivity of total lymphocyte count as a surrogate marker to identify antepartum and postpartum Indian women who require antiretroviral therapy
    Amita Gupta
    Division of Infectious Diseases Center for Clinical Global Health Education, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA
    J Acquir Immune Defic Syndr 46:338-42. 2007
    ....
  22. ncbi request reprint High HIV prevalence among a high-risk subgroup of women attending sexually transmitted infection clinics in Pune, India
    Shruti H Mehta
    Department of Epidemiology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21202, USA
    J Acquir Immune Defic Syndr 41:75-80. 2006
    ..To investigate changes over a decade in prevalence and correlates of HIV among high-risk women attending sexually transmitted infection (STI) clinics in Pune, India, who deny a history of commercial sex work (CSW)...
  23. ncbi request reprint Adherence to antiretroviral therapy and virologic suppression among HIV-infected persons receiving care in private clinics in Mumbai, India
    Bijal Shah
    Department of Emergency Medicine, Carolinas Medical Center, Charlotte, NC, USA
    Clin Infect Dis 44:1235-44. 2007
    ..Adherence to antiretroviral therapy (ART) and correlates of adherence and virologic suppression among human immunodeficiency virus (HIV)-infected persons receiving ART in private, outpatient clinics in India is unknown...
  24. ncbi request reprint Impact of maternal HIV coinfection on the vertical transmission of hepatitis C virus: a meta-analysis
    Chelsea B Polis
    Department of Population, Family and Reproductive Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA
    Clin Infect Dis 44:1123-31. 2007
    ..Observational studies suggest that maternal human immunodeficiency virus (HIV)-hepatitis C virus (HCV) coinfection is associated with increased odds of vertical HCV transmission. We performed a meta-analysis to summarize current evidence...
  25. ncbi request reprint Knowledge, attitudes, and practices of antiretroviral therapy among HIV-infected adults attending private and public clinics in India
    Suneil R Ramchandani
    Department of Internal Medicine, National Naval Medical Center, Bethesda, Maryland, USA
    AIDS Patient Care STDS 21:129-42. 2007
    ..In order to make widespread dissemination of ART effective in India, further educational and programmatic efforts are likely needed to optimize access, treatment awareness, and compliance among patients with HIV...
  26. ncbi request reprint Clinically significant anemia in HIV-infected pregnant women in India is not a major barrier to zidovudine use for prevention of maternal-to-child transmission
    Gita Sinha
    Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA
    J Acquir Immune Defic Syndr 45:210-7. 2007
    ..To determine the prevalence of anemia (serum hemoglobin <10 g/dL) and assess zidovudine use and toxicity in HIV-positive pregnant women in India...