Marcelo E Guerin

Summary

Publications

  1. pmc Substrate-induced conformational changes in the essential peripheral membrane-associated mannosyltransferase PimA from mycobacteria: implications for catalysis
    Marcelo E Guerin
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado 80523 1682, USA
    J Biol Chem 284:21613-25. 2009
  2. pmc New insights into the early steps of phosphatidylinositol mannoside biosynthesis in mycobacteria: PimB' is an essential enzyme of Mycobacterium smegmatis
    Marcelo E Guerin
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado 80523 1682, USA
    J Biol Chem 284:25687-96. 2009
  3. pmc Molecular basis of phosphatidyl-myo-inositol mannoside biosynthesis and regulation in mycobacteria
    Marcelo E Guerin
    Unidad de Biofisica, Centro Mixto Consejo Superior de Investigaciones Cientificas Universidad del País Vasco Euskal Herriko Unibertsitatea, Leioa, Bizkaia 48940, Spain
    J Biol Chem 285:33577-83. 2010
  4. pmc Conformational plasticity of the essential membrane-associated mannosyltransferase PimA from mycobacteria
    David Giganti
    From the Unidad de Biofísica, Consejo Superior de Investigaciones Científicas Universidad del País Vasco Euskal Herriko Unibertsitatea, Barrio Sarriena s n, Leioa, Bizkaia 48940, Spain
    J Biol Chem 288:29797-808. 2013
  5. pmc Preliminary crystallographic analysis of GpgS, a key glucosyltransferase involved in methylglucose lipopolysaccharide biosynthesis in Mycobacterium tuberculosis
    Petra Gest
    Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523 168, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 64:1121-4. 2008
  6. pmc Chapter 2: Biogenesis of the cell wall and other glycoconjugates of Mycobacterium tuberculosis
    Devinder Kaur
    Department of Microbiology, Immunology and Pathology, Mycobacteria Research Laboratories, Colorado State University, Fort Collins, CO 80523 1682, USA
    Adv Appl Microbiol 69:23-78. 2009
  7. pmc Mechanistic insights into the retaining glucosyl-3-phosphoglycerate synthase from mycobacteria
    Saioa Urresti
    Unidad de Biofisica, Centro Mixto Consejo Superior de Investigaciones Cientificas Universidad del País Vasco Euskal Herriko Unibertsitatea, Barrio Sarriena s n, Leioa, Bizkaia, 48940, Spain
    J Biol Chem 287:24649-61. 2012
  8. pmc The Redox State Regulates the Conformation of Rv2466c to Activate the Antitubercular Prodrug TP053
    David Albesa-Jové
    From the Unidad de Biofísica, Centro Mixto Consejo Superior de Investigaciones Cientificas, Universidad del País Vasco Euskal Herriko Unibertsitatea CSIC, UPV EHU, Barrio Sarriena s n, Leioa, Bizkaia 48940, Spain, the Departamento de Bioquímica, Universidad del Pais Vasco, Leioa, Bizkaia 48940, Spain, the IKERBASQUE, Basque Foundation for Science, 48013 Bilbao, Spain
    J Biol Chem 290:31077-89. 2015
  9. pmc Molecular Basis of Membrane Association by the Phosphatidylinositol Mannosyltransferase PimA Enzyme from Mycobacteria
    Ane Rodrigo-Unzueta
    From the Unidad de Biofísica, Centro Mixto Consejo Superior de Investigaciones Científicas Universidad del País Vasco Euskal Herriko Unibertsitatea CSIC, UPV EHU, Barrio Sarriena s n, Leioa, Bizkaia 48940, Spain, the Departamento de Bioquímica, Universidad del Pais Vasco, 48940 Leioa, Vizcaya, Spain
    J Biol Chem 291:13955-63. 2016
  10. doi request reprint Secondary structure reshuffling modulates glycosyltransferase function at the membrane
    David Giganti
    1 Institut Pasteur, Unité de Microbiologie Structurale, CNRS UMR 3528, Paris, France 2 Unidad de Biofisica, Centro Mixto Consejo Superior de Investigaciones Científicas Universidad del País Vasco Euskal Herriko Unibertsitatea CSIC, UPV EHU, Leioa, Bizkaia, Spain 3 Departamento de Bioquímica, Universidad del Pais Vasco, Spain
    Nat Chem Biol 11:16-8. 2015

Collaborators

Detail Information

Publications18

  1. pmc Substrate-induced conformational changes in the essential peripheral membrane-associated mannosyltransferase PimA from mycobacteria: implications for catalysis
    Marcelo E Guerin
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado 80523 1682, USA
    J Biol Chem 284:21613-25. 2009
    ..The proposed mechanism has implications for the comprehension of the peripheral membrane-binding GTs at the molecular level...
  2. pmc New insights into the early steps of phosphatidylinositol mannoside biosynthesis in mycobacteria: PimB' is an essential enzyme of Mycobacterium smegmatis
    Marcelo E Guerin
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado 80523 1682, USA
    J Biol Chem 284:25687-96. 2009
    ..The essentiality of these three enzymes emphasizes the interest of novel anti-tuberculosis drugs targeting the initial steps of PIM biosynthesis...
  3. pmc Molecular basis of phosphatidyl-myo-inositol mannoside biosynthesis and regulation in mycobacteria
    Marcelo E Guerin
    Unidad de Biofisica, Centro Mixto Consejo Superior de Investigaciones Cientificas Universidad del País Vasco Euskal Herriko Unibertsitatea, Leioa, Bizkaia 48940, Spain
    J Biol Chem 285:33577-83. 2010
    ....
  4. pmc Conformational plasticity of the essential membrane-associated mannosyltransferase PimA from mycobacteria
    David Giganti
    From the Unidad de Biofísica, Consejo Superior de Investigaciones Científicas Universidad del País Vasco Euskal Herriko Unibertsitatea, Barrio Sarriena s n, Leioa, Bizkaia 48940, Spain
    J Biol Chem 288:29797-808. 2013
    ..Based on these results and on our previous experimental data, we propose a model wherein the conformational transitions are important for the mannosyltransferase to interact with the donor and acceptor substrates/membrane. ..
  5. pmc Preliminary crystallographic analysis of GpgS, a key glucosyltransferase involved in methylglucose lipopolysaccharide biosynthesis in Mycobacterium tuberculosis
    Petra Gest
    Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523 168, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 64:1121-4. 2008
    ..85, b = 98.85, c = 127.64 A, and diffracted to 2.6 A resolution. GpgS-UDP complex crystals belonged to space group I4, with unit-cell parameters a = 98.32, b = 98.32, c = 127.96 A, and diffracted to 3.0 A resolution...
  6. pmc Chapter 2: Biogenesis of the cell wall and other glycoconjugates of Mycobacterium tuberculosis
    Devinder Kaur
    Department of Microbiology, Immunology and Pathology, Mycobacteria Research Laboratories, Colorado State University, Fort Collins, CO 80523 1682, USA
    Adv Appl Microbiol 69:23-78. 2009
    ..In this review, the laboratory personnel who have been the focal point of some to these developments review recent progress towards a comprehensive understanding of the basic physiology and functions of the cell wall of M. tuberculosis...
  7. pmc Mechanistic insights into the retaining glucosyl-3-phosphoglycerate synthase from mycobacteria
    Saioa Urresti
    Unidad de Biofisica, Centro Mixto Consejo Superior de Investigaciones Cientificas Universidad del País Vasco Euskal Herriko Unibertsitatea, Barrio Sarriena s n, Leioa, Bizkaia, 48940, Spain
    J Biol Chem 287:24649-61. 2012
    ..This model of action may be applicable to a broad range of GT-A glycosyltransferases...
  8. pmc The Redox State Regulates the Conformation of Rv2466c to Activate the Antitubercular Prodrug TP053
    David Albesa-Jové
    From the Unidad de Biofísica, Centro Mixto Consejo Superior de Investigaciones Cientificas, Universidad del País Vasco Euskal Herriko Unibertsitatea CSIC, UPV EHU, Barrio Sarriena s n, Leioa, Bizkaia 48940, Spain, the Departamento de Bioquímica, Universidad del Pais Vasco, Leioa, Bizkaia 48940, Spain, the IKERBASQUE, Basque Foundation for Science, 48013 Bilbao, Spain
    J Biol Chem 290:31077-89. 2015
    ..Our studies suggest that similar structural changes might have a functional role in other members of the thioredoxin-fold superfamily. ..
  9. pmc Molecular Basis of Membrane Association by the Phosphatidylinositol Mannosyltransferase PimA Enzyme from Mycobacteria
    Ane Rodrigo-Unzueta
    From the Unidad de Biofísica, Centro Mixto Consejo Superior de Investigaciones Científicas Universidad del País Vasco Euskal Herriko Unibertsitatea CSIC, UPV EHU, Barrio Sarriena s n, Leioa, Bizkaia 48940, Spain, the Departamento de Bioquímica, Universidad del Pais Vasco, 48940 Leioa, Vizcaya, Spain
    J Biol Chem 291:13955-63. 2016
    ..Altogether, we propose a model in which conformational transitions observed in PimA might reflect a molten globule state that confers to PimA, a higher affinity toward the dynamic and highly fluctuating lipid bilayer...
  10. doi request reprint Secondary structure reshuffling modulates glycosyltransferase function at the membrane
    David Giganti
    1 Institut Pasteur, Unité de Microbiologie Structurale, CNRS UMR 3528, Paris, France 2 Unidad de Biofisica, Centro Mixto Consejo Superior de Investigaciones Científicas Universidad del País Vasco Euskal Herriko Unibertsitatea CSIC, UPV EHU, Leioa, Bizkaia, Spain 3 Departamento de Bioquímica, Universidad del Pais Vasco, Spain
    Nat Chem Biol 11:16-8. 2015
    ..These structural changes modulate catalysis and are promoted by interactions of the protein with anionic phospholipids in the membrane. ..
  11. pmc Initiation of methylglucose lipopolysaccharide biosynthesis in mycobacteria
    Devinder Kaur
    Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO, USA
    PLoS ONE 4:e5447. 2009
    ..Towards defining the roles of these polysaccharides in mycobacterial physiology, we generated knock-out mutations of genes in their putative biosynthetic pathways...
  12. doi request reprint Structural Basis of Glycogen Biosynthesis Regulation in Bacteria
    Javier O Cifuente
    Structural Biology Unit, CIC bioGUNE, Bizkaia Technology Park, 48160 Derio, Spain Unidad de Biofísica, Centro Mixto Consejo Superior de Investigaciones Cientificas, Universidad del País Vasco Euskal Herriko Unibertsitatea CSIC, UPV EHU, Barrio Sarriena s n, Leioa, Bizkaia, 48940, Spain Departamento de Bioquímica, Universidad del Pais Vasco, 48940 Leioa, Bizkaia, Spain
    Structure 24:1613-22. 2016
    ..We propose a model in which the energy reporters regulate EcAGPase catalytic activity by intra-protomer interactions and inter-protomer crosstalk, with a sensory motif and two regulatory loops playing a prominent role. ..
  13. pmc Structure-function relationships of membrane-associated GT-B glycosyltransferases
    David Albesa-Jové
    Unidad de Biofisica, Centro Mixto Consejo Superior de Investigaciones Científicas Universidad del País Vasco Euskal Herriko Unibertsitatea CSIC, UPV EHU, Barrio Sarriena s n, Leioa, Bizkaia 48940, Spain
    Glycobiology 24:108-24. 2014
    ....
  14. doi request reprint Structural basis of phosphatidyl-myo-inositol mannosides biosynthesis in mycobacteria
    Enea Sancho-Vaello
    Unidad de Biofisica, Centro Mixto Consejo Superior de Investigaciones Cientificas, Universidad del Pais Vasco, Leioa, Bizkaia 48940, Spain Departamento de Bioquímica, Universidad del Pais Vasco, Leioa, Bizkaia 48940, Spain Universidad del País Vasco Euskal Herriko Unibertsitatea CSIC, UPV EHU, Barrio Sarriena s n, Leioa, Bizkaia 48940, Spain
    Biochim Biophys Acta . 2016
    ..This article is part of a Special Issue entitled: Bacterial Lipids edited by Russell E. Bishop...
  15. pmc Glycolytic and non-glycolytic functions of Mycobacterium tuberculosis fructose-1,6-bisphosphate aldolase, an essential enzyme produced by replicating and non-replicating bacilli
    María de la Paz Santangelo
    Mycobacteria Research Laboratories, Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado 80523 1682, USA
    J Biol Chem 286:40219-31. 2011
    ..Altogether, our results highlight the potential of FBA-tb as a novel therapeutic target against both replicating and non-replicating bacilli...
  16. pmc Structural basis for selective recognition of acyl chains by the membrane-associated acyltransferase PatA
    David Albesa-Jové
    Unidad de Biofisica, Consejo Superior de Investigaciones Científicas Universidad del País Vasco Euskal Herriko Unibertsitatea CSIC, UPV EHU, Barrio Sarriena s n, Leioa, 48940 Bizkaia, Spain
    Nat Commun 7:10906. 2016
    ..Our studies establish the mechanistic basis of substrate/membrane recognition and catalysis for an important family of acyltransferases, providing exciting possibilities for inhibitor design. ..
  17. doi request reprint Rv2466c mediates the activation of TP053 to kill replicating and non-replicating Mycobacterium tuberculosis
    David Albesa-Jové
    Unidad de Biofisica, Centro Mixto Consejo Superior de Investigaciones Científicas Universidad del País Vasco Euskal Herriko Unibertsitatea CSIC, UPV EHU, Leioa, Bizkaia 48940, Spain
    ACS Chem Biol 9:1567-75. 2014
    ..Altogether our experimental data provide insights into the molecular mechanism underlying the anti-mycobacterial activity of TP-based compounds, paving the way for future drug development programmes. ..
  18. doi request reprint The conformational plasticity of glycosyltransferases
    David Albesa-Jové
    Structural Biology Unit, CIC bioGUNE, Bizkaia Technology Park, 48160 Derio, Spain Unidad de Biofísica, Centro Mixto Consejo Superior de Investigaciones Cientificas, Universidad del País Vasco Euskal Herriko Unibertsitatea CSIC, UPV EHU, Barrio Sarriena s n, Leioa, Bizkaia 48940, Spain Departamento de Bioquímica, Universidad del Pais Vasco, Leioa, Bizkaia 48940, Spain IKERBASQUE, Basque Foundation for Science, 48011 Bilbao, Spain
    Curr Opin Struct Biol 40:23-32. 2016
    ..The elucidations of the molecular mechanisms by which these events govern the function and substrate specificity of GTs represent a major challenge...