Paul Christakopoulos

Summary

Affiliation: National Technical University of Athens
Country: Greece

Publications

  1. ncbi request reprint Purification and characterization of two low molecular mass alkaline xylanases from Fusarium oxysporum F3
    P Christakopoulos
    Department of Biochemistry, Physiology and Microbiology, Faculty of Sciences, University of Gent, Belgium
    J Biotechnol 51:181-9. 1996
  2. ncbi request reprint The alkaline xylanase III from Fusarium oxysporum F3 belongs to family F/10
    P Christakopoulos
    Department of Chemical Engineering, National Technical University of Athens, Greece
    Carbohydr Res 302:191-5. 1997
  3. doi request reprint Purification, characterization and mass spectrometric sequencing of a thermophilic glucuronoyl esterase from Sporotrichum thermophile
    Christina Vafiadi
    BIOtechMASS Unit, Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, Athens, Greece
    FEMS Microbiol Lett 296:178-84. 2009
  4. ncbi request reprint The feruloyl esterase system of Talaromyces stipitatus: determining the hydrolytic and synthetic specificity of TsFaeC
    Christina Vafiadi
    Biotechnology Laboratory, Chemical Engineering Department, National Technical University of Athens, 5 Iroon Polytechniou Str, Zografou Campus, 15700 Athens, Greece
    J Biotechnol 125:210-21. 2006
  5. doi request reprint Functional expression of a thermophilic glucuronyl esterase from Sporotrichum thermophile: identification of the nucleophilic serine
    Evangelos Topakas
    BIOtechMASS Unit, Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 5 Iroon Polytechniou Str, Zografou Campus, Athens 15780, Greece
    Appl Microbiol Biotechnol 87:1765-72. 2010
  6. doi request reprint Feruloyl esterase-catalysed synthesis of glycerol sinapate using ionic liquids mixtures
    Christina Vafiadi
    BIOtechMASS Unit, Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 5 Iroon Polytechniou Street, Zografou Campus, 15700 Athens, Greece
    J Biotechnol 139:124-9. 2009
  7. doi request reprint Factors affecting cellulose and hemicellulose hydrolysis of alkali treated brewers spent grain by Fusarium oxysporum enzyme extract
    Charilaos Xiros
    BIOtechMASS Unit, Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 9 Iroon Polytechniou Street, Zografou Campus, 15700 Athens, Greece
    Bioresour Technol 102:1688-96. 2011
  8. doi request reprint Evaluation of Fusarium oxysporum cellulolytic system for an efficient hydrolysis of hydrothermally treated wheat straw
    Charilaos Xiros
    Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 9 Iroon Polytechniou Street, Zografou Campus, 15700 Athens, Greece
    Bioresour Technol 100:5362-5. 2009
  9. doi request reprint Cloning, characterization and functional expression of an alkalitolerant type C feruloyl esterase from Fusarium oxysporum
    Maria Moukouli
    BIOtechMASS Unit, Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 5 Iroon Polytechniou Str, Zografou Campus, 15700 Athens, Greece
    Appl Microbiol Biotechnol 79:245-54. 2008
  10. ncbi request reprint Biochemical and catalytic properties of an endoxylanase purified from the culture filtrate of Sporotrichum thermophile
    Petros Katapodis
    Department of Chemical Engineering, National Technical University of Athens, 5 Iroon Polytechniou Ave, Zografou Campus, 15700 Athens, Greece
    Carbohydr Res 338:1881-90. 2003

Collaborators

  • Evangelos Topakas
  • Craig B Faulds
  • W Nerinckx
  • Mats Sandgren
  • Harry J Gilbert
  • Lisbeth Olsson
  • Gurdyal S Besra
  • Michael Karpusas
  • Mária Vršanská
  • James W Murray
  • Richard J Lewis
  • Gianni Panagiotou
  • David Navarro
  • Nikolas Fokialakis
  • Christina Vafiadi
  • Charilaos Xiros
  • Maria Dimarogona
  • Anastasia Zerva
  • Constantinos Katsimpouras
  • Diomi Mamma
  • Petros Katapodis
  • Dimitris Kekos
  • Maria Vardakou
  • Maria Moukouli
  • Thomas Paschos
  • Elisavet Kourtoglou
  • Dimitris G Hatzinikolaou
  • Anthi C Karnaouri
  • Maria Despoina Charavgi
  • Leonidas Matsakas
  • Anthi Karnaouri
  • Lefki Maria Papaspyridi
  • Basil J Macris
  • Styliani Kalantzi
  • Peter Biely
  • Nikolaos Manos
  • Pericles Petropoulos
  • Georgios I Zervakis
  • Stamatina Vouyiouka
  • Maria Kanelli
  • Efstratios Nikolaivits
  • Anaïs Bénarouche
  • Jean Guy Berrin
  • Ioanna Taouki
  • Evangelia D Chrysina
  • Alexandros Leandros Skaltsounis
  • Nektarios Aligiannis
  • George E Anasontzis
  • Victoria R Nahmias
  • Apostolos Alissandratos
  • Edwin J Bakx
  • Arjan Narbad
  • Carmen Nueno Palop
  • Luke J Alderwick
  • Henk A Schols
  • Michael Gasson
  • Dimitris Petroutsos
  • Eleni Papadopoulou
  • James Flint
  • Constantine J Philippopoulos
  • Spiridon Gerontas
  • Marc Claeyssens
  • George Panayotou
  • Martina Samiotaki
  • Emanuel Kalogeris

Detail Information

Publications48

  1. ncbi request reprint Purification and characterization of two low molecular mass alkaline xylanases from Fusarium oxysporum F3
    P Christakopoulos
    Department of Biochemistry, Physiology and Microbiology, Faculty of Sciences, University of Gent, Belgium
    J Biotechnol 51:181-9. 1996
    ..Thus the purified enzymes appeared to be true endo-beta-1,4-xylanases. The amino terminal sequences of xylanases I and II show to homology. Xylanase I shows high similarity with alkaline low molecular mass xylanases of family G/11...
  2. ncbi request reprint The alkaline xylanase III from Fusarium oxysporum F3 belongs to family F/10
    P Christakopoulos
    Department of Chemical Engineering, National Technical University of Athens, Greece
    Carbohydr Res 302:191-5. 1997
    ..Thus the purified enzyme appears to be a true endo-beta-1,4-xylanase. Partial amino acid analysis of xylanase III shows high sequence homology with xylanases of family F/10...
  3. doi request reprint Purification, characterization and mass spectrometric sequencing of a thermophilic glucuronoyl esterase from Sporotrichum thermophile
    Christina Vafiadi
    BIOtechMASS Unit, Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, Athens, Greece
    FEMS Microbiol Lett 296:178-84. 2009
    ..51. This work represents the first example of the purification and identification of a thermophilic glucuronoyl esterase from S. thermophile...
  4. ncbi request reprint The feruloyl esterase system of Talaromyces stipitatus: determining the hydrolytic and synthetic specificity of TsFaeC
    Christina Vafiadi
    Biotechnology Laboratory, Chemical Engineering Department, National Technical University of Athens, 5 Iroon Polytechniou Str, Zografou Campus, 15700 Athens, Greece
    J Biotechnol 125:210-21. 2006
    ..The reaction parameters affecting the feruloylation rate and the conversion of the enzymatic synthesis, such as the composition of the reaction media, temperature, substrate and enzyme concentration have been investigated...
  5. doi request reprint Functional expression of a thermophilic glucuronyl esterase from Sporotrichum thermophile: identification of the nucleophilic serine
    Evangelos Topakas
    BIOtechMASS Unit, Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 5 Iroon Polytechniou Str, Zografou Campus, Athens 15780, Greece
    Appl Microbiol Biotechnol 87:1765-72. 2010
    ..The putative serine has been mutated, and the corresponding enzyme has been expressed in P. pastoris to prove that the candidate nucleophilic residue is responsible for catalyzing the enzymatic reaction...
  6. doi request reprint Feruloyl esterase-catalysed synthesis of glycerol sinapate using ionic liquids mixtures
    Christina Vafiadi
    BIOtechMASS Unit, Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 5 Iroon Polytechniou Street, Zografou Campus, 15700 Athens, Greece
    J Biotechnol 139:124-9. 2009
    ..1+/-0.3% and 89.5+/-1.1% of its antioxidant activity against low-density lipoprotein oxidation, when added at concentrations of 10 and 60muM, respectively, in the assay mixture...
  7. doi request reprint Factors affecting cellulose and hemicellulose hydrolysis of alkali treated brewers spent grain by Fusarium oxysporum enzyme extract
    Charilaos Xiros
    BIOtechMASS Unit, Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 9 Iroon Polytechniou Street, Zografou Campus, 15700 Athens, Greece
    Bioresour Technol 102:1688-96. 2011
    ..All the factors investigated were combined in a mathematical model describing and predicting alkali treated brewers spent grain conversion by F. oxysporum enzyme extract...
  8. doi request reprint Evaluation of Fusarium oxysporum cellulolytic system for an efficient hydrolysis of hydrothermally treated wheat straw
    Charilaos Xiros
    Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 9 Iroon Polytechniou Street, Zografou Campus, 15700 Athens, Greece
    Bioresour Technol 100:5362-5. 2009
    ..A kinetic model incorporating dynamic adsorption, enzymatic hydrolysis, and product inhibition was developed. The model predicted very satisfactorily the experimental data...
  9. doi request reprint Cloning, characterization and functional expression of an alkalitolerant type C feruloyl esterase from Fusarium oxysporum
    Maria Moukouli
    BIOtechMASS Unit, Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 5 Iroon Polytechniou Str, Zografou Campus, 15700 Athens, Greece
    Appl Microbiol Biotechnol 79:245-54. 2008
    ..The esterase showed broad pH stability making it an important candidate for alkaline applications such as pulp treatment in the paper industry...
  10. ncbi request reprint Biochemical and catalytic properties of an endoxylanase purified from the culture filtrate of Sporotrichum thermophile
    Petros Katapodis
    Department of Chemical Engineering, National Technical University of Athens, 5 Iroon Polytechniou Ave, Zografou Campus, 15700 Athens, Greece
    Carbohydr Res 338:1881-90. 2003
    ..The enzyme is irreversibly inhibited by series of omega-epoxyalkyl glycosides of D-xylopyranose. The results suggest that the endoxylanase from S. thermophile has catalytic properties similar to the enzymes belonging to family 11...
  11. ncbi request reprint Enzymatic synthesis of butyl hydroxycinnamates and their inhibitory effects on LDL-oxidation
    Christina Vafiadi
    Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 5 Iroon Polytechniou Street, Zografou Campus, 15700 Athens, Greece
    J Biotechnol 133:497-504. 2008
    ....
  12. ncbi request reprint Mode of action of family 10 and 11 endoxylanases on water-unextractable arabinoxylan
    Maria Vardakou
    Biotechnology Laboratory, Chemical Engineering Department, National Technical University of Athens, 5 Iroon Polytechniou Str, Zografou Campus, 15700 Athens, Greece
    Int J Biol Macromol 33:129-34. 2003
    ..thermophile, which liberated a feruloyl arabinoxylotrisaccharide (FAX3). These results indicated that different factors govern WU-AX breakdown by the two endoxylanases...
  13. doi request reprint Constitutive expression, purification and characterization of a phosphoglucomutase from Fusarium oxysporum
    Elisavet Kourtoglou
    Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 9 Iroon Polytechniou Str, Zografou Campus, 15780 Zografou, Greece
    Enzyme Microb Technol 48:217-24. 2011
    ..10mM; K(m)(G1,6 diP), 1.03 μM; k(cat)/K(m)(G1P), 443 mM(-1)min(-1) and k(cat)/K(m)(G1,6 diP), 42,860 mM(-1)min(-1). The enzyme was considered to follow a Ping Pong substituted enzyme or enzyme isomerization mechanism...
  14. doi request reprint The structure of a novel glucuronoyl esterase from Myceliophthora thermophila gives new insights into its role as a potential biocatalyst
    Maria Despoina Charavgi
    Institute of Biology, Medicinal Chemistry and Biotechnology, National Hellenic Research Foundation, Athens, Greece
    Acta Crystallogr D Biol Crystallogr 69:63-73. 2013
    ..The knowledge derived offers novel insights into the key structural elements that drive the hydrolysis of glucuronic acid esters...
  15. ncbi request reprint Regioselective esterase-catalyzed feruloylation of L-arabinobiose
    Christina Vafiadi
    Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 5 Iroon Polytechniou Str, Zografou Campus, GR 15700 Athens, Greece
    Carbohydr Res 341:1992-7. 2006
    ..The reaction parameters affecting the feruloylation rate and conversion of the enzymatic synthesis, such as the composition of the reaction medium, substrate and enzyme concentration, have been investigated...
  16. doi request reprint Factors affecting ferulic acid release from Brewer's spent grain by Fusarium oxysporum enzymatic system
    Charilaos Xiros
    BIOtechMASS Unit, Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 5 Iroon Polytechniou Str, Zografou Campus, Athens 15780, Greece
    Bioresour Technol 100:5917-21. 2009
    ..The protease treatment prior to hydrolysis by monoenzymes enhanced FA release about 100%, while, for the first time in literature, FA in solution found to have a significant inhibitory effect on FAE activity and on total FA release...
  17. ncbi request reprint Cell bound and extracellular glucose oxidases from Aspergillus niger BTL: evidence for a secondary glycosylation mechanism
    Dimitris G Hatzinikolaou
    Laboratory of Microbiology, Department of Botany, School of Biology, National and Kapodistrian University of Athens, Panepistimioupoli Zografou, Athens, Greece
    Appl Biochem Biotechnol 142:29-43. 2007
    ..These results suggest A. niger BTL probably possesses a secondary glycosylation mechanism that increases the stability of the excreted GOX...
  18. doi request reprint Carbohydrate esterases of family 2 are 6-O-deacetylases
    Evangelos Topakas
    Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, Athens, Greece
    FEBS Lett 584:543-8. 2010
    ..e., to the primary hydroxyl group of mono- and disaccharides. Xylose and xylooligosaccharides did not serve as acetyl group acceptors, therefore the CE-2 enzymes appear to be 6-O-deacetylases...
  19. doi request reprint Expression, characterization and structural modelling of a feruloyl esterase from the thermophilic fungus Myceliophthora thermophila
    Evangelos Topakas
    School of Chemical Engineering, National Technical University of Athens, Athens, Greece
    Appl Microbiol Biotechnol 94:399-411. 2012
    ..Prediction of the secondary structure of MtFae1a was performed in the PSIPRED server whilst modelling the 3D structure was accomplished by the use of the HH 3D structure prediction server...
  20. doi request reprint Lignin boosts the cellulase performance of a GH-61 enzyme from Sporotrichum thermophile
    Maria Dimarogona
    BIOtechMASS Unit, Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 5 Iroon Polytechniou Str, Zografou Campus, 15700 Athens, Greece
    Bioresour Technol 110:480-7. 2012
    ..The synergistic activity exhibited by StCel61a and its dependence on reducing substances provide guidelines for process design towards the production of economically viable bioethanol...
  21. ncbi request reprint A family 10 Thermoascus aurantiacus xylanase utilizes arabinose decorations of xylan as significant substrate specificity determinants
    Maria Vardakou
    Biotechnology Laboratory, Chemical Engineering Department, National Technical University of Athens, 5 Iroon Polytechniou Str, Zografou Campus, 15780, Athens, Greece
    J Mol Biol 352:1060-7. 2005
    ..These data indicate that the sugar decorations of xylans in the T.aurantiacus family 10 xylanase, rather than simply being accommodated, can be significant substrate specificity determinants...
  22. doi request reprint Hydrolysis and fermentation of brewer's spent grain by Neurospora crassa
    Charilaos Xiros
    Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 9 Iroon Polytechniou Strasse, Zografou Campus, 15700 Athens, Greece
    Bioresour Technol 99:5427-35. 2008
    ..Alkali pre-treated BG was used for ethanol production. A yield of about 74 g of ethanol kg(-1) dry BG (5,6 g L(-1)) was obtained under optimum conditions (aeration 0.1 vvm, pre-treatment with 1g NaOH 10 g(-1)dry BG)...
  23. ncbi request reprint Removal of 1,3-dichloro2-propanol and 3-chloro1,2-propanediol by the whole cell system of pseudomonas putida DSM 437
    Diomi Mamma
    Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, Zografou Campus, 157 80, Zografou, Greece
    J Environ Sci Health A Tox Hazard Subst Environ Eng 41:303-13. 2006
    ..For 1,3-DCP, it ranged from 40.55 h at 200 mg . L(-1) to 53.28 h at 500 mg . L(-1) while the respected values for 3-CPD were 33.39 and 68.91 h...
  24. doi request reprint Cloning and optimized expression of a GH-11 xylanase from Fusarium oxysporum in Pichia pastoris
    Maria Moukouli
    BIOtechMASS Unit, Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 5 Iroon Polytechniou Str, Zografou Campus, 15700 Athens, Greece
    N Biotechnol 28:369-74. 2011
    ..Under optimum culture conditions, production of the recombinant xylanase increased by 50%, reaching a final yield of 170 U/ml, underpinning aeration as the most important factor in improving enzyme production...
  25. ncbi request reprint Structural characterisation by ESI-MS of feruloylated arabino-oligosaccharides synthesised by chemoenzymatic esterification
    Christina Vafiadi
    Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 5 Iroon Polytechniou Str, Zografou Campus, 15700, Athens Greece
    Molecules 12:1367-75. 2007
    ..The chemoenzymatic synthesis of feruloylated arabino-oligosaccharides has been achieved, using a feruloyl esterase type C from Sporotrichum thermophile (StFaeC). The structure of the feruloylated products was confirmed by ESI-MS(n)...
  26. ncbi request reprint Comparison of mesophilic and thermophilic feruloyl esterases: characterization of their substrate specificity for methyl phenylalkanoates
    Evangelos Topakas
    Biotechnology Laboratory, Chemical Engineering Department, National Technical University of Athens, 5 Iroon Polytechniou Str, Zografou Campus, 15700 Athens, Greece
    J Biotechnol 115:355-66. 2005
    ..The thermophilic type-B esterase had a lower catalytic efficiency than its mesophilic counterpart, but released more ferulic acid from plant cell walls...
  27. doi request reprint Recalcitrant polysaccharide degradation by novel oxidative biocatalysts
    Maria Dimarogona
    Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 5 Iroon Polytechniou Str, Zografou Campus, 15700, Athens, Greece
    Appl Microbiol Biotechnol 97:8455-65. 2013
    ..In this review, we present the recent findings concerning the mechanism of action of these oxidative enzymes and identify issues and questions to be addressed in the future. ..
  28. doi request reprint Fermentation of liquefacted hydrothermally pretreated sweet sorghum bagasse to ethanol at high-solids content
    Leonidas Matsakas
    BIOtechMASS Unit, Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 5 Iroon Polytechniou Str, Zografou Campus, 15700 Athens, Greece
    Bioresour Technol 127:202-8. 2013
    ..43 g/L and a volumetric productivity of 1.88 g/Lh. The addition of extra enzymes at the start up of SSF enhanced both ethanol concentration and volumetric productivity by 16% and 17% after 12 and 24h saccharification, respectively...
  29. ncbi request reprint Chemoenzymatic synthesis of feruloyl D-arabinose as a potential anti-mycobacterial agent
    Christina Vafiadi
    Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 5 Iroon Polytechniou Str, Zografou Campus, Athens, 15700, Greece
    Biotechnol Lett 29:1771-4. 2007
    ..About 45% conversion of D: -arabinose to the feruloylated derivative was achieved. The compound had an MIC value against Mycobacterium bovis BCG of 25 microg/ml...
  30. ncbi request reprint Induction, purification, and characterization of two extracellular alpha-L-arabinofuranosidases from Fusarium oxysporum
    Gianni Panagiotou
    Chemical Engineering Department, National Technical University of Athens, Greece
    Can J Microbiol 49:639-44. 2003
    ..The enzymes were not active on substrates containing ferulic acid ester linked to C-5 and C-2 linkages of pNPA showing that phenolic substituents of pNPA sterically hindered the action of ABFs...
  31. ncbi request reprint In vitro three-stage continuous fermentation of wheat arabinoxylan fractions and induction of hydrolase activity by the gut microflora
    Maria Vardakou
    Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 5 Iroon Polytechniou Str, Zografou Campus, 15780 Athens, Greece Institute of Food Research, Norwich Research Park, Colney, NR4 7UA Norwich, UK
    Int J Biol Macromol 41:584-9. 2007
    ....
  32. doi request reprint A thermostable GH26 endo-β-mannanase from Myceliophthora thermophila capable of enhancing lignocellulose degradation
    Constantinos Katsimpouras
    Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 9 Iroon Polytechniou Str, Zografou Campus, Athens, 15780, Greece
    Appl Microbiol Biotechnol 100:8385-97. 2016
    ..5 L and Novozyme® 188 resulted in enhanced enzymatic hydrolysis of pretreated beechwood sawdust, improving the release of total reducing sugars and glucose by 13 and 12 %, respectively. ..
  33. doi request reprint Submerged fermentation of the edible mushroom Pleurotus ostreatus in a batch stirred tank bioreactor as a promising alternative for the effective production of bioactive metabolites
    Lefki Maria Papaspyridi
    BIOtechMASS Unit, Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 9 Iroon Polytechniou Street, Zografou Campus, GR 15700 Athens, Greece
    Molecules 17:2714-24. 2012
    ..This work indicates the great potential of the established bioprocess for the production of P. ostreatus mycelia with enhanced metabolic profile...
  34. doi request reprint Acetic acid-catalyzed hydrothermal pretreatment of corn stover for the production of bioethanol at high-solids content
    Constantinos Katsimpouras
    Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 9 Iroon Polytechniou Str, Zografou Campus, 15780, Athens, Greece
    Bioprocess Biosyst Eng 39:1415-23. 2016
    ..The threshold of ethanol concentration of 4 % (w/w), which is required to reduce the cost of ethanol distillation, was surpassed by the addition of extra enzymes at the start up of SSF achieving this way ethanol titer of 41.5 g/L. ..
  35. doi request reprint Bioconversion of Biomass-Derived Phenols Catalyzed by Myceliophthora thermophila Laccase
    Anastasia Zerva
    Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 5 Iroon Polytechniou Str, Zografou Campus, Athens 15780, Greece
    Molecules 21:. 2016
    ..The herein studied green synthesis of enzymatically synthesized phenol polymers or biological active compounds could be exploited as an alternative synthetic route targeting a variety of applications. ..
  36. doi request reprint Degradation of olive mill wastewater by the induced extracellular ligninolytic enzymes of two wood-rot fungi
    Anastasia Zerva
    Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 5 Iroon Polytechniou Str, Zografou Campus, Athens 15780, Greece
    J Environ Manage . 2016
    ..Hence, P. citrinopileatus and I. lacteus appear as potent degraders of OMWW with the ability to use the effluent as a substrate for the production of biotechnologically important enzymes and valuable fungal glucans...
  37. doi request reprint Structural and functional studies of a Fusarium oxysporum cutinase with polyethylene terephthalate modification potential
    Maria Dimarogona
    Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 5 Iroon Polytechniou Str, Zografou Campus, Athens 15780, Greece Department of Chemistry and Biotechnology, Swedish University of Agricultural Science, SE 75007 Uppsala, Sweden
    Biochim Biophys Acta 1850:2308-17. 2015
    ..These biocatalysts have recently attracted increased biotechnological interest due to their potential to modify and degrade polyethylene terephthalate (PET), as well as other synthetic polymers...
  38. pmc Ethanol effect on metabolic activity of the ethalogenic fungus Fusarium oxysporum
    Thomas Paschos
    Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 9 Iroon Polytechniou Str, Zografou Campus, Athens, 5780, Greece
    BMC Biotechnol 15:15. 2015
    ..Although this fungus has been studied a lot as a cell factory, regarding applications for the production of bioethanol and other high added value products, no systematic study has been performed concerning its ethanol tolerance levels...
  39. doi request reprint Enzymatic synthesis of model substrates recognized by glucuronoyl esterases from Podospora anserina and Myceliophthora thermophila
    Constantinos Katsimpouras
    Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 5 Iroon Polytechniou Str, Zografou Campus, Athens, 15780, Greece
    Appl Microbiol Biotechnol 98:5507-16. 2014
    ..The experimental results were corroborated by computational docking of the synthesized substrate analogues. We show that the nature of the alcohol portion of the hydrolyzed ester influences the catalytic efficiency of the two GEs...
  40. doi request reprint Effect of pectate lyase bioscouring on physical, chemical and low-stress mechanical properties of cotton fabrics
    Styliani Kalantzi
    Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 9 Iroon Polytechniou Street, Zografou Campus, 157 80 Zografou, Attica, Greece
    Bioresour Technol 99:8185-92. 2008
    ..Bending and shear rigidity, compressional resilience, as well as, extensibility of enzymatically treated cotton fabrics could be efficiently predicted by means of a single independent variable, the crystallinity index...
  41. ncbi request reprint Enzymic production of a feruloylated oligosaccharide with antioxidant activity from wheat flour arabinoxylan
    Petros Katapodis
    Biotechnology Laboratory, Chemical Engineering Department, National Technical University of Athens, Zografou Campus, Greece
    Eur J Nutr 42:55-60. 2003
    ..Many studies have dealt with the isolation of feruloylated oligosaccharides from Gramineae by treatment with polysaccharide hydrolysing enzymes...
  42. ncbi request reprint Combined photo-assisted and biological treatment of industrial oily wastewater
    Diomi Mamma
    Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, Zografou, Greece
    J Environ Sci Health A Tox Hazard Subst Environ Eng 39:729-40. 2004
    ..The overall degradation of phenol was 78% while the 59% and 84% of the initial o-cresol and p-cresol respectively, were removed from the wastewater. The combined process resulted in 72% of COD removal...
  43. pmc Enhanced ethanol production from brewer's spent grain by a Fusarium oxysporum consolidated system
    Charilaos Xiros
    BIOtechMASS Unit, Biotechnology Laboratory, Chemical Engineering Department, National Technical University of Athens, Iroon Polytechniou st, Zografou Campus, 15700, Athens, Greece
    Biotechnol Biofuels 2:4. 2009
    ..oxysporum. The alkali pretreatment of BG and different aeration levels during the ethanol production stage were studied for the optimization of the ethanol production by F. oxysporum...
  44. doi request reprint Characterization and application of a novel class II thermophilic peroxidase from Myceliophthora thermophila in biosynthesis of polycatechol
    Anastasia Zerva
    Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 5 Iroon Polytechniou Str, Zografou Campus, Athens 15780, Greece
    Enzyme Microb Technol 75:49-56. 2015
    ..The ability of the peroxidase to polymerize catechol at high superoxide concentrations, together with its high thermostability and substrate specificity, indicate a potential commercial significance of the enzyme...
  45. doi request reprint Screening and purification of recombinant lignocellulolytic enzymes
    Evangelos Topakas
    Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, Athens, Greece
    Methods Mol Biol 1129:517-26. 2014
    ....
  46. doi request reprint Cloning, expression, and characterization of a thermostable GH7 endoglucanase from Myceliophthora thermophila capable of high-consistency enzymatic liquefaction
    Anthi C Karnaouri
    Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 5 Iroon Polytechniou Str, Zografou Campus, 15700, Athens, Greece
    Appl Microbiol Biotechnol 98:231-42. 2014
    ..High thermostability of the endoglucanase reflects potential commercial significance of the enzyme...
  47. pmc Cloning, expression and characterization of an ethanol tolerant GH3 β-glucosidase from Myceliophthora thermophila
    Anthi Karnaouri
    Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, Athens, Greece
    Peerj 1:e46. 2013
    ....
  48. ncbi request reprint Fungal multienzyme production on industrial by-products of the citrus-processing industry
    Diomi Mamma
    Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 9 Iroon Polytechniou Str, Zografou Campus, 157 80 Zografou, Attica, Greece
    Bioresour Technol 99:2373-83. 2008
    ....