John Ziebuhr

Summary

Country: Germany

Publications

  1. ncbi request reprint The coronavirus replicase
    J Ziebuhr
    Institute of Virology and Immunology, University of Wurzburg, Versbacher Str 7, 97078 Wurzburg, Germany
    Curr Top Microbiol Immunol 287:57-94. 2005
  2. ncbi request reprint Molecular biology of severe acute respiratory syndrome coronavirus
    John Ziebuhr
    Institute of Virology and Immunology, University of Wurzburg, Versbacher Str 7, 97078 Wurzburg, Germany
    Curr Opin Microbiol 7:412-9. 2004
  3. pmc Major genetic marker of nidoviruses encodes a replicative endoribonuclease
    Konstantin A Ivanov
    Institute of Virology and Immunology, University of Wurzburg, Versbacher Strasse 7, 97078 Wurzburg, Germany
    Proc Natl Acad Sci U S A 101:12694-9. 2004
  4. ncbi request reprint A new lead for nonpeptidic active-site-directed inhibitors of the severe acute respiratory syndrome coronavirus main protease discovered by a combination of screening and docking methods
    Ulrich Kaeppler
    Institute of Pharmacy and Food Chemistry, University of Wurzburg, Am Hubland, D 97074 Wurzburg, Germany
    J Med Chem 48:6832-42. 2005
  5. pmc ADP-ribose-1"-monophosphatase: a conserved coronavirus enzyme that is dispensable for viral replication in tissue culture
    Akos Putics
    Institute of Virology and Immunology, University of Wurzburg, Germany
    J Virol 79:12721-31. 2005
  6. pmc Discovery of an RNA virus 3'->5' exoribonuclease that is critically involved in coronavirus RNA synthesis
    Ekaterina Minskaia
    Institute of Virology and Immunology, University of Wurzburg, 97078 Wurzburg, Germany
    Proc Natl Acad Sci U S A 103:5108-13. 2006
  7. ncbi request reprint ADP-ribose-1"-phosphatase activities of the human coronavirus 229E and SARS coronavirus X domains
    Akos Putics
    University of Wurzburg, Germany
    Adv Exp Med Biol 581:93-6. 2006
  8. ncbi request reprint Mechanisms and enzymes involved in SARS coronavirus genome expression
    Volker Thiel
    Institute of Virology and Immunology, University of Wurzburg, Versbacher Str 7, 97078 Wurzburg, Germany
    J Gen Virol 84:2305-15. 2003
  9. ncbi request reprint Identification of protease and ADP-ribose 1''-monophosphatase activities associated with transmissible gastroenteritis virus non-structural protein 3
    Akos Putics
    Institute of Virology and Immunology, University of Wurzburg, Versbacher Strasse 7, 97078 Wurzburg, Germany
    J Gen Virol 87:651-6. 2006
  10. ncbi request reprint Conservation of substrate specificities among coronavirus main proteases
    Annette Hegyi
    Institute of Virology and Immunology, University of Wurzburg, Versbacher Strasse 7, 97078 Wurzburg, Germany
    J Gen Virol 83:595-9. 2002

Collaborators

  • Alexander E Gorbalenya
  • Witold Filipowicz
  • Ekaterina Minskaia
  • Bruno Canard
  • Carsten Schmuck
  • Burkhard Ludewig
  • E J Snijder
  • V Thiel
  • Duanqing Pei
  • Peter J Bredenbeek
  • Yi Guan
  • J A Cowley
  • Leo Lm Poon
  • Guangxia Gao
  • Luis Enjuanes
  • Akos Putics
  • Konstantin A Ivanov
  • Rolf Hilgenfeld
  • Kanchan Anand
  • Marie Pierre Egloff
  • Annette Hegyi
  • Tobias Hertzig
  • Jeroen R Mesters
  • Rachel Ulferts
  • Christian Cambillau
  • Valerie Campanacci
  • Rajesh Ponnusamy
  • Stefano Ricagno
  • Heike Schütze
  • Alexander Breuning
  • Knut Baumann
  • Barbara Schelle
  • Nikolaus Stiefl
  • Ulrich Kaeppler
  • HaiTao Yang
  • Tanja Schirmeister
  • Radim Vicik
  • Anja Seybert
  • Erika Martina
  • Markus Schiller
  • Sonja Bayer
  • Stuart G Siddell
  • Didier Nurizzo
  • Helene Dutartre
  • Helene Malet
  • Bruno Coutard
  • Jutta Slaby
  • Bernd Hoffmann
  • Antoine Frangeul
  • Tero Ahola
  • Thomas C Mettenleiter
  • Ralf Moll
  • Maarit Heinonen
  • Arnaud Gruez
  • Harald Granzow
  • Wenxue Liang
  • Mark Bartlam
  • Dawei Ma
  • Franziska Schulz
  • Zhe Zhou
  • Kwok Yung Yuen
  • Luet Wong
  • Weiqing Xie
  • Werner Schmitz
  • Xiaoyu Xue
  • Kailin Yang
  • Björn Degel
  • Jing Ma
  • Daniel Rupprecht
  • Leonie C van Dinten
  • Clara C Posthuma
  • Saijuan Chen
  • Zihe Rao
  • Jonathan Hall
  • Qi Zhao
  • Zhu Chen
  • Elke Scandella
  • Jessika C Dobbe
  • Yvonne van der Meer
  • Mikhail Rozanov
  • Parvesh Wadhwani
  • Gottfried J Palm
  • Agnes Friebe

Detail Information

Publications30

  1. ncbi request reprint The coronavirus replicase
    J Ziebuhr
    Institute of Virology and Immunology, University of Wurzburg, Versbacher Str 7, 97078 Wurzburg, Germany
    Curr Top Microbiol Immunol 287:57-94. 2005
    ....
  2. ncbi request reprint Molecular biology of severe acute respiratory syndrome coronavirus
    John Ziebuhr
    Institute of Virology and Immunology, University of Wurzburg, Versbacher Str 7, 97078 Wurzburg, Germany
    Curr Opin Microbiol 7:412-9. 2004
    ..These recent advances in coronavirus research have important implications for the development of anti-SARS drugs and vaccines...
  3. pmc Major genetic marker of nidoviruses encodes a replicative endoribonuclease
    Konstantin A Ivanov
    Institute of Virology and Immunology, University of Wurzburg, Versbacher Strasse 7, 97078 Wurzburg, Germany
    Proc Natl Acad Sci U S A 101:12694-9. 2004
    ..Substitution of D6408 by Ala was shown to abolish viral RNA synthesis, demonstrating that NendoU has critical functions in viral replication and transcription...
  4. ncbi request reprint A new lead for nonpeptidic active-site-directed inhibitors of the severe acute respiratory syndrome coronavirus main protease discovered by a combination of screening and docking methods
    Ulrich Kaeppler
    Institute of Pharmacy and Food Chemistry, University of Wurzburg, Am Hubland, D 97074 Wurzburg, Germany
    J Med Chem 48:6832-42. 2005
    ..3 muM. Since the novel lead compound does not target the S1', S1, and S2 subsites of the enzyme's substrate-binding pockets, there is room for improvement that underlines the lead character of compound 6b...
  5. pmc ADP-ribose-1"-monophosphatase: a conserved coronavirus enzyme that is dispensable for viral replication in tissue culture
    Akos Putics
    Institute of Virology and Immunology, University of Wurzburg, Germany
    J Virol 79:12721-31. 2005
    ..The biological significance of the novel enzymatic activity in vivo remains to be investigated...
  6. pmc Discovery of an RNA virus 3'->5' exoribonuclease that is critically involved in coronavirus RNA synthesis
    Ekaterina Minskaia
    Institute of Virology and Immunology, University of Wurzburg, 97078 Wurzburg, Germany
    Proc Natl Acad Sci U S A 103:5108-13. 2006
    ..Taken together, the study identifies an RNA virus ExoN activity that is involved in the synthesis of multiple RNAs from the exceptionally large genomic RNA templates of CoVs...
  7. ncbi request reprint ADP-ribose-1"-phosphatase activities of the human coronavirus 229E and SARS coronavirus X domains
    Akos Putics
    University of Wurzburg, Germany
    Adv Exp Med Biol 581:93-6. 2006
  8. ncbi request reprint Mechanisms and enzymes involved in SARS coronavirus genome expression
    Volker Thiel
    Institute of Virology and Immunology, University of Wurzburg, Versbacher Str 7, 97078 Wurzburg, Germany
    J Gen Virol 84:2305-15. 2003
    ..The availability of recombinant forms of key replicative enzymes of SARS coronavirus should pave the way for high-throughput screening approaches to identify candidate inhibitors in compound libraries...
  9. ncbi request reprint Identification of protease and ADP-ribose 1''-monophosphatase activities associated with transmissible gastroenteritis virus non-structural protein 3
    Akos Putics
    Institute of Virology and Immunology, University of Wurzburg, Versbacher Strasse 7, 97078 Wurzburg, Germany
    J Gen Virol 87:651-6. 2006
    ..Expression and characterization of a range of bacterially expressed forms of this enzyme suggest that the active X domain comprises pp1a/pp1ab residues Asp(1320)-Ser(1486)...
  10. ncbi request reprint Conservation of substrate specificities among coronavirus main proteases
    Annette Hegyi
    Institute of Virology and Immunology, University of Wurzburg, Versbacher Strasse 7, 97078 Wurzburg, Germany
    J Gen Virol 83:595-9. 2002
    ....
  11. ncbi request reprint Rapid identification of coronavirus replicase inhibitors using a selectable replicon RNA
    Tobias Hertzig
    Institute of Virology and Immunology, University of Wurzburg, Wurzburg, Germany
    J Gen Virol 85:1717-25. 2004
    ..Once this technology is adapted to SARS-CoV replicon RNAs, it will allow high throughput screening for SARS-CoV replicase inhibitors without the need to grow infectious SARS-CoV...
  12. pmc A complex zinc finger controls the enzymatic activities of nidovirus helicases
    Anja Seybert
    Institute of Virology and Immunology, University of Wurzburg, Versbacher Str 7, 97078 Wurzburg, Germany
    J Virol 79:696-704. 2005
    ..Collectively, our data suggest that the ZBD is critically involved in nidovirus replication and transcription by modulating the enzymatic activities of the helicase domain and other, yet unknown, mechanisms...
  13. pmc Multiple enzymatic activities associated with severe acute respiratory syndrome coronavirus helicase
    Konstantin A Ivanov
    Institute of Virology and Immunology, University of Wurzburg, Wurzburg, Germany
    J Virol 78:5619-32. 2004
    ..Finally, we established that, in SARS-CoV-infected Vero E6 cells, nsp13 localizes to membranes that appear to be derived from the endoplasmic reticulum and are the likely site of SARS-CoV RNA synthesis...
  14. ncbi request reprint The coronavirus replicase: insights into a sophisticated enzyme machinery
    John Ziebuhr
    University of Wurzburg, Germany
    Adv Exp Med Biol 581:3-11. 2006
  15. ncbi request reprint Screening of electrophilic compounds yields an aziridinyl peptide as new active-site directed SARS-CoV main protease inhibitor
    Erika Martina
    Institute of Pharmacy and Food Chemistry, University of Wurzburg, Germany
    Bioorg Med Chem Lett 15:5365-9. 2005
    ..Among the trans-configured aziridine-2,3-dicarboxylates the Gly-Gly-containing peptide 2c was found to be the most potent inhibitor...
  16. pmc The 3C-like proteinase of an invertebrate nidovirus links coronavirus and potyvirus homologs
    John Ziebuhr
    Institute of Virology and Immunology, University of Wurzburg, Germany
    J Virol 77:1415-26. 2003
    ..The properties of the GAV 3CL(pro) define a novel RNA virus proteinase variant that bridges the gap between the distantly related chymotrypsin-like cysteine proteinases of coronaviruses and potyviruses...
  17. pmc Human coronavirus 229E nonstructural protein 13: characterization of duplex-unwinding, nucleoside triphosphatase, and RNA 5'-triphosphatase activities
    Konstantin A Ivanov
    Institute of Virology and Immunology, University of Wurzburg, Wurzburg, Germany
    J Virol 78:7833-8. 2004
    ..Using the NTPase active site, HCoV-229E nsp13 also mediates RNA 5'-triphosphatase activity, which may be involved in the capping of viral RNAs...
  18. ncbi request reprint Mutational analysis of the active centre of coronavirus 3C-like proteases
    Annette Hegyi
    Institute of Virology and Immunology, University of Wurzburg, Versbacher Strasse 7, 97078 Wurzburg, Germany
    J Gen Virol 83:581-93. 2002
    ..These data lead us to conclude that both the catalytic systems and substrate-binding pockets of coronavirus main proteases differ from those of other RNA virus 3C and 3C-like proteases...
  19. pmc Design of wide-spectrum inhibitors targeting coronavirus main proteases
    HaiTao Yang
    Tsinghua IBP Joint Research Group for Structural Biology, Tsinghua University, Beijing, China
    PLoS Biol 3:e324. 2005
    ..Further modification could rapidly lead to the discovery of a single agent with clinical potential against existing and possible future emerging CoV-related diseases...
  20. pmc Structure of coronavirus main proteinase reveals combination of a chymotrypsin fold with an extra alpha-helical domain
    Kanchan Anand
    Department of Structural Biology and Crystallography, Institute of Molecular Biotechnology, D 07745 Jena, Germany
    EMBO J 21:3213-24. 2002
    ..The study illustrates that RNA viruses have evolved unprecedented variations of the classical chymotrypsin fold...
  21. ncbi request reprint Coronavirus main proteinase (3CLpro) structure: basis for design of anti-SARS drugs
    Kanchan Anand
    Institute of Biochemistry, University of Lubeck, D 23538 Lubeck, Germany
    Science 300:1763-7. 2003
    ..Molecular modeling suggests that available rhinovirus 3Cpro inhibitors may be modified to make them useful for treating SARS...
  22. pmc Characterization of White bream virus reveals a novel genetic cluster of nidoviruses
    Heike Schütze
    The Queen s University of Belfast, School of Biomedical Sciences, 97 Lisburn Road, Belfast BT9 7BL, United Kingdom
    J Virol 80:11598-609. 2006
    ..The study adds to the amazing diversity of nidoviruses and appeals for a more extensive characterization of nonmammalian nidoviruses to better understand the evolution of these largest known RNA viruses...
  23. pmc Crystal structure and mechanistic determinants of SARS coronavirus nonstructural protein 15 define an endoribonuclease family
    Stefano Ricagno
    Case 925, Ecole d Ingénieurs de Luminy, Architecture et Fonction des Macromolecules Biologiques, Unite Mixte de Recherche 6098, Centre National de la Recherche Scientifique and Universités d Aix Marseille I et II, 13288 Marseille Cedex 9, France
    Proc Natl Acad Sci U S A 103:11892-7. 2006
    ....
  24. ncbi request reprint Unique and conserved features of genome and proteome of SARS-coronavirus, an early split-off from the coronavirus group 2 lineage
    Eric J Snijder
    Department of Medical Microbiology, Leiden University Medical Center, Room L4 34, Albinusdreef 2, PO Box 9600, 2300 RC, Leiden, The Netherlands
    J Mol Biol 331:991-1004. 2003
    ..Furthermore, together with previously described viral enzymes, they will be important targets for the design of antiviral strategies aimed at controlling the further spread of SARS-CoV...
  25. ncbi request reprint [Basically SARS is not a surprise. Coronaviruses lie in wait almost everywhere]
    John Ziebuhr
    Institut fur Virologie und Immunbiologie, Universitat Wurzburg
    Krankenpfl J 41:114-5. 2003
  26. ncbi request reprint SARS--unprecedented global response to a newly emerging disease
    John Ziebuhr
    Int J Med Microbiol 293:229-31. 2003
  27. pmc Human coronavirus 229E papain-like proteases have overlapping specificities but distinct functions in viral replication
    John Ziebuhr
    Centre for Cancer Research and Cell Biology, School of Biomedical Sciences, The Queen s University of Belfast, UK, and Kantonal Hospital St Gallen, Research Department, 9007 St Gallen, Switzerland
    J Virol 81:3922-32. 2007
    ..Functional and evolutionary implications of the differential amino-terminal polyprotein-processing pathways among the main CoV lineages are discussed...
  28. pmc Structural and functional basis for ADP-ribose and poly(ADP-ribose) binding by viral macro domains
    Marie Pierre Egloff
    Centre National de la Recherche Scientifique and Universités d Aix Marseille I et II, UMR 6098, Architecture et Fonction des Macromolecules Biologiques, Ecole Supérieure d Ingénieurs de Luminy Case 925, France
    J Virol 80:8493-502. 2006
    ..Collectively, these results suggest to further evaluate the role of viral macro domains in host response to viral infection...
  29. ncbi request reprint Nidovirales: evolving the largest RNA virus genome
    Alexander E Gorbalenya
    Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center, LUMC E4 P, P O Box 9600, 2300 RC Leiden, The Netherlands
    Virus Res 117:17-37. 2006
    ..The acquisition of these enzymes may have improved the low fidelity of RNA replication to allow genome expansion and give rise to the ancestors of small and, subsequently, large nidoviruses...
  30. ncbi request reprint Non structural proteins 8 and 9 of human coronavirus 229E
    Rajesh Ponnusamy
    University of Lubeck, Germany
    Adv Exp Med Biol 581:49-54. 2006