Genomes and Genes
- The DNA topoisomerase I binding protein topors as a novel cellular target for SUMO-1 modification: characterization of domains necessary for subcellular localization and sumolationStefan Weger
Department of Virology, Institute of Infectious Diseases, Free University of Berlin, Hindenburgdamm 27, 12203 Berlin, Germany
Exp Cell Res 290:13-27. 2003..One SUMO-1 acceptor site at lysine residue 560 could be identified within this region. However, sumolation-deficient Topors mutants showed that sumolation obviously is not required for localization to nuclear speckles...
- Topors acts as a SUMO-1 E3 ligase for p53 in vitro and in vivoStefan Weger
Institute of Infectious Diseases, Department of Virology, Charite Campus Benjamin Franklin, Free University of Berlin, Hindenburgdamm 27, 12203 Berlin, Germany
FEBS Lett 579:5007-12. 2005..In HeLa cells, Topors induced p53 sumoylation was accompanied by an increase in endogenous p53 protein levels. Furthermore, Topors enhances the sumoylation of a variety of other, yet unidentified, cellular proteins...
- Topors, a p53 and topoisomerase I binding protein, interacts with the adeno-associated virus (AAV-2) Rep78/68 proteins and enhances AAV-2 gene expressionStefan Weger
Institut für Infektionsmedizin, Abteilung Virologie, Freie Universitat Berlin, Hindenburgdamm 27, D 12203 Berlin, Germany
J Gen Virol 83:511-6. 2002..Overexpression of Topors stimulated AAV gene expression in the absence of helper virus, suggesting a function of Topors as a transcriptional regulator...
- The E3 ligase Topors induces the accumulation of polysumoylated forms of DNA topoisomerase I in vitro and in vivoEva Hammer
Institute of Virology, Charite Campus Benjamin Franklin, Free University of Berlin, Hindenburgdamm 27, 12203 Berlin, Germany
FEBS Lett 581:5418-24. 2007..Experiments with TOP1 point mutants strongly suggest that the high-molecular weight conjugates represent SUMO-1 chains formed on a limited number of SUMO-1 acceptor sites...
- Identification of a cytoplasmic interaction partner of the large regulatory proteins Rep78/Rep68 of adeno-associated virus type 2 (AAV-2)Stefan Weger
Institut fur Virologie, Charite Campus Benjamin Franklin, Freie Universitat Berlin, Hindenburgdamm 27, 12203 Berlin, Germany
Virology 362:192-206. 2007..Our study expands the panel of already identified nuclear Rep interaction partners to a cytoplasmic protein, which raises the awareness that important steps in the AAV life cycle may be regulated in this compartment...
- SUMO-1 modification regulates the protein stability of the large regulatory protein Rep78 of adeno associated virus type 2 (AAV-2)Stefan Weger
Institut für Infektionsmedizin, Abteilung Virologie, Charite Campus Benjamin Franklin, Freie Universitat Berlin, 12203 Berlin, Germany
Virology 330:284-94. 2004..This finding implicates a role for sumolation in the regulation of Rep78 protein stability that is assumed to be critical for the establishment and maintenance of AAV latency...
- Packaging of human chromosome 19-specific adeno-associated virus (AAV) integration sites in AAV virions during AAV wild-type and recombinant AAV vector productionDaniela Hüser
Department of Virology, Institute of Infectious Diseases, Free University of Berlin, 12203 Berlin, Germany
J Virol 77:4881-7. 2003..Apparently, AAV latency established by site-specific integration and the helper virus-dependent, productive AAV cycle are more closely related than previously thought...
- Role of the herpes simplex virus helicase-primase complex during adeno-associated virus DNA replicationHeiko Slanina
Institut fur Virologie, Charite Universitatsmedizin Berlin, Campus Benjamin Franklin, Hindenburgdamm 27, 12203 Berlin, Germany
J Virol 80:5241-50. 2006....
- ssDNA-dependent colocalization of adeno-associated virus Rep and herpes simplex virus ICP8 in nuclear replication domainsRegine Heilbronn
Institut für Infektionsmedizin, Abt Virologie, Charite Campus Benjamin Franklin, Freie Universitat Berlin, Germany
Nucleic Acids Res 31:6206-13. 2003..This novel mechanism for recruiting components of a functional replication complex directs AAV to subnuclear HSV replication compartments where the HSV replication complex can replicate the AAV genome...
- Kinetics and frequency of adeno-associated virus site-specific integration into human chromosome 19 monitored by quantitative real-time PCRDaniela Hüser
Department of Virology, Institute of Infectious Diseases, Free University of Berlin, Berlin, Germany
J Virol 76:7554-9. 2002..Use of this quantitative real-time PCR will greatly facilitate the study of the early steps of wild-type and recombinant AAV vector integration...
- Viral vectors for gene transfer: current status of gene therapeuticsRegine Heilbronn
Institute of Virology, Charite Universitatsmedizin Berlin, Hindenburgdamm 27, 12203 Berlin, Germany
Handb Exp Pharmacol . 2010..The focus is on production methods suitable for biopharmaceutical upscaling and for downstream processing. Quality control measures and biological safety considerations for the use of vectors in clinical trials are discussed...
- OneBac: platform for scalable and high-titer production of adeno-associated virus serotype 1-12 vectors for gene therapyMario Mietzsch
1 Institute of Virology, Campus Benjamin Franklin, Charite Universitatsmedizin Berlin, 12203 Berlin, Germany
Hum Gene Ther 25:212-22. 2014..Thus, OneBac combines full AAV serotype options with the capacity for stable scale-up production, the current bottleneck for the transition of AAV from gene therapy trials to routine clinical treatment...
- SUMOylation modulates transcriptional repression by TRPS1Frank J Kaiser
Institut fur Humangenetik, Universitatsklinikum Essen, D 45122 Essen, Germany
Biol Chem 388:381-90. 2007..These results identify SUMOylation at the TRPS1-RD as a major mechanism that regulates the function of TRPS1...
- A bidirectional Tet-dependent promotor construct regulating the expression of E1A for tight control of oncolytic adenovirus replicationHenry Fechner
Department of Cardiology and Pulmology, Charite Universitatsmedizin Berlin, Campus Benjamin Franklin, Hindenburgdamm 30, 12200 Berlin, Germany
J Biotechnol 127:560-74. 2007..Future improvement of the Dox-dependent E1A regulation cassette should lead to the generation of an oAdV well suited to meet the demands for a highly regulated and efficient oncolytic virus for in vivo applications...
- An improved Tet-On regulatable FasL-adenovirus vector system for lung cancer therapyIsaac Sipo
Department of Cardiology and Pneumology, Charite Universitatsmedizin Berlin, Campus Benjamin Franklin, Hindenburgdamm 30, 12200 Berlin Germany
J Mol Med (Berl) 84:215-25. 2006..These data show that the novel TRE-Tight1 promoter has a high potential for closely controlled and efficient expression of cytotoxic genes in AdV-based anti-cancer approaches...