Genomes and Genes
Peter J Verveer
- High-resolution three-dimensional imaging of large specimens with light sheet-based microscopyPeter J Verveer
Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
Nat Methods 4:311-3. 2007..The ability to rapidly image large samples at high resolution with minimal photodamage provides new opportunities especially for the study of subcellular processes in large living specimens...
- Quantitative microscopy and systems biology: seeing the whole picturePeter J Verveer
Department of Systemic Cell Biology, Max Planck Institute of Molecular Physiology, Otto Hahn Strasse 11, 44227, Dortmund, Germany
Histochem Cell Biol 130:833-43. 2008..Combined with computational approaches they allow us to obtain insights in the reaction-diffusion processes that determine biological function on the scale of cells...
- Global analysis of time correlated single photon counting FRET-FLIM dataHernan E Grecco
Department of Systemic Cell Biology, Max Planck Institute of Molecular Physiology, Otto Hahn Strasse 11, 44227 Dortmund, Germany
Opt Express 17:6493-508. 2009..The proposed method is efficient and robust, and can be routinely applied to analyze FRET-FLIM data acquired in intact cells...
- FRET in cell biology: still shining in the age of super-resolution?Hernan E Grecco
Department of Systemic Cell Biology, Max Planck Institute of Molecular Physiology, Otto Hahn Strasse 11, D 44227 Dortmund, Germany
Chemphyschem 12:484-90. 2011..Herein we review the major applications in biological FRET imaging and we discuss the possibilities and challenges in the super-resolution era...
- An acylation cycle regulates localization and activity of palmitoylated Ras isoformsOliver Rocks
Department of Structural Biology, Max Planck Institute for Molecular Physiology, Otto Hahn Strasse 11, 44227 Dortmund, Germany
Science 307:1746-52. 2005..The de/reacylation cycle also initiates Ras activation at the Golgi by transport of PM-localized Ras guanosine triphosphate. Different de/repalmitoylation kinetics account for isoform-specific activation responses to growth factors...
- Growth factor-induced MAPK network topology shapes Erk response determining PC-12 cell fateSilvia D M Santos
European Molecular Biology Laboratory EMBL, Cell Biology and Biophysics, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
Nat Cell Biol 9:324-30. 2007..These results show that growth factor context determines the topology of the MAPK signalling network and that the resulting dynamics govern cell fate...
- Single Particle Tracking Reveals that EGFR Signaling Activity Is Amplified in Clathrin-Coated PitsJenny Ibach
Department of Systemic Cell Biology, Max Planck Institute of Molecular Physiology, Dortmund, Germany
PLoS ONE 10:e0143162. 2015..These results show that amplification of EGFR phosphorylation by receptor clustering in clathrin-coated pits supports signal activation at the plasma membrane. ..
- Red-edge anisotropy microscopy enables dynamic imaging of homo-FRET between green fluorescent proteins in cellsAnthony Squire
Cell Biology and Cell Biophysics Program, European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
J Struct Biol 147:62-9. 2004....
- Cdt1 associates dynamically with chromatin throughout G1 and recruits Geminin onto chromatinGeorgia Xouri
Laboratory of General Biology, School of Medicine, University of Patras, Patras, Greece
EMBO J 26:1303-14. 2007..We propose that dynamic Cdt1-chromatin associations and the recruitment of Geminin to chromatin provide spatio-temporal control of the licensing process...
- Imaging sites of receptor dephosphorylation by PTP1B on the surface of the endoplasmic reticulumFawaz G Haj
Cancer Biology Program, Division of Hematology Oncology, Department of Medicine, Beth Israel Deaconess Medical Center, Boston, MA 02115, USA
Science 295:1708-11. 2002..Most of the RTKs activated at the cell surface showed interaction with PTP1B after internalization, establishing that RTK activation and inactivation are spatially and temporally partitioned within cells...