S A Wudy

Summary

Affiliation: University of Giessen
Country: Germany

Publications

  1. ncbi request reprint Gas chromatography-mass spectrometry profiling of steroids in times of molecular biology
    S A Wudy
    Steroid Research Unit, Center of Child and Adolescent Medicine, Justus Liebig University, Feulgenstrasse 12, 35392 Giessen, Germany
    Horm Metab Res 36:415-22. 2004
  2. ncbi request reprint Children with idiopathic short stature are poor eaters and have decreased body mass index
    Stefan A Wudy
    Department of General Pediatrics, Center of Child and Adolescent Medicine, Justus Liebig University, Giessen, Germany
    Pediatrics 116:e52-7. 2005
  3. ncbi request reprint Determination of 11-deoxycortisol (Reichstein's compound S) in human plasma by clinical isotope dilution mass spectrometry using benchtop gas chromatography-mass selective detection
    Stefan A Wudy
    Steroid Laboratory, Children s Hospital, Justus Liebig University of Giessen, Giessen, Germany
    Steroids 67:851-7. 2002
  4. ncbi request reprint Determination of 17alpha-hydroxypregnenolone in human plasma by routine isotope dilution mass spectrometry using benchtop gas chromatography-mass selective detection
    S A Wudy
    Steroid Laboratory, Department of Pediatrics, University of Ulm Prittwitzstr 43, D 89070, Ulm Donau, Germany
    Steroids 66:759-62. 2001
  5. ncbi request reprint Sexual dimorphism in cortisol secretion starts after age 10 in healthy children: urinary cortisol metabolite excretion rates during growth
    Stefan A Wudy
    Steroid Research Unit, Center of Child and Adolescent Medicine, Justus Liebig University, Feulgenstr 12, D 35392 Giessen, Germany
    Am J Physiol Endocrinol Metab 293:E970-6. 2007
  6. ncbi request reprint Profiling steroid hormones in amniotic fluid of midpregnancy by routine stable isotope dilution/gas chromatography-mass spectrometry: reference values and concentrations in fetuses at risk for 21-hydroxylase deficiency
    S A Wudy
    Department of Pediatrics, University of Ulm, Germany
    J Clin Endocrinol Metab 84:2724-8. 1999
  7. ncbi request reprint Serum cortisol concentrations in ill preterm infants less than 30 weeks gestational age
    M Heckmann
    Department of Pediatrics, University of Ulm, Germany
    Acta Paediatr 89:1098-103. 2000
  8. ncbi request reprint Assessing cortisol production in preterm infants: do not dispose of the nappies
    Matthias Heckmann
    Department of General Pediatrics and Neonatology, Center of Child and Adolescent Medicine, Justus Liebig University, 35392 Giessen, Germany
    Pediatr Res 57:412-8. 2005
  9. doi request reprint Profiling oestrogens and testosterone in human urine by stable isotope dilution/benchtop gas chromatography-mass spectrometry
    Philipp Hoffmann
    Department of General Pediatrics and Neonatology, Center of Child and Adolescent Medicine, Justus Liebig University, Giessen, Germany
    Steroids 75:1067-74. 2010
  10. ncbi request reprint Persistent high activity of the fetal adrenal cortex in preterm infants: is there a clinical significance?
    Matthias Heckmann
    Department of General Pediatrics and Neonatology, Center of Child and Adolescent Medicine, Justus Liebig University, Giessen, Germany
    J Pediatr Endocrinol Metab 19:1303-12. 2006

Collaborators

Detail Information

Publications37

  1. ncbi request reprint Gas chromatography-mass spectrometry profiling of steroids in times of molecular biology
    S A Wudy
    Steroid Research Unit, Center of Child and Adolescent Medicine, Justus Liebig University, Feulgenstrasse 12, 35392 Giessen, Germany
    Horm Metab Res 36:415-22. 2004
    ..Clinical GC-MS profiling of steroids is also highly recommended as a reasonable initial diagnostic approach--especially in unclear situations--avoiding uncritical and expensive attempts at molecular diagnostic testing...
  2. ncbi request reprint Children with idiopathic short stature are poor eaters and have decreased body mass index
    Stefan A Wudy
    Department of General Pediatrics, Center of Child and Adolescent Medicine, Justus Liebig University, Giessen, Germany
    Pediatrics 116:e52-7. 2005
    ..In children with idiopathic short stature (ISS), studies investigating body mass index (BMI) or parameters of satiety regulation are scarce, and studies analyzing eating behavior are lacking...
  3. ncbi request reprint Determination of 11-deoxycortisol (Reichstein's compound S) in human plasma by clinical isotope dilution mass spectrometry using benchtop gas chromatography-mass selective detection
    Stefan A Wudy
    Steroid Laboratory, Children s Hospital, Justus Liebig University of Giessen, Giessen, Germany
    Steroids 67:851-7. 2002
    ....
  4. ncbi request reprint Determination of 17alpha-hydroxypregnenolone in human plasma by routine isotope dilution mass spectrometry using benchtop gas chromatography-mass selective detection
    S A Wudy
    Steroid Laboratory, Department of Pediatrics, University of Ulm Prittwitzstr 43, D 89070, Ulm Donau, Germany
    Steroids 66:759-62. 2001
    ..3% and interassay precision CV was 5.6%. Requiring small amounts of plasma, the rapid, convenient work up and the application of bench top GC/MS instrumentation proved our method suitable for routine clinical use in adults and children...
  5. ncbi request reprint Sexual dimorphism in cortisol secretion starts after age 10 in healthy children: urinary cortisol metabolite excretion rates during growth
    Stefan A Wudy
    Steroid Research Unit, Center of Child and Adolescent Medicine, Justus Liebig University, Feulgenstr 12, D 35392 Giessen, Germany
    Am J Physiol Endocrinol Metab 293:E970-6. 2007
    ..In conclusion, the varying metabolic need for cortisol during normal growth may have implications for future improvements in glucocorticoid replacement therapy...
  6. ncbi request reprint Profiling steroid hormones in amniotic fluid of midpregnancy by routine stable isotope dilution/gas chromatography-mass spectrometry: reference values and concentrations in fetuses at risk for 21-hydroxylase deficiency
    S A Wudy
    Department of Pediatrics, University of Ulm, Germany
    J Clin Endocrinol Metab 84:2724-8. 1999
    ..Our study provides the first mass spectrometric reference data on amniotic fluid steroid concentrations and underscores the high accuracy of prenatal hormonal diagnosis of 21-hydroxylase deficiency...
  7. ncbi request reprint Serum cortisol concentrations in ill preterm infants less than 30 weeks gestational age
    M Heckmann
    Department of Pediatrics, University of Ulm, Germany
    Acta Paediatr 89:1098-103. 2000
    ..003 for all comparisons) with the following sequence: A < C < B, but not between B1 and B2, as clinical variables were not different between the subgroups...
  8. ncbi request reprint Assessing cortisol production in preterm infants: do not dispose of the nappies
    Matthias Heckmann
    Department of General Pediatrics and Neonatology, Center of Child and Adolescent Medicine, Justus Liebig University, 35392 Giessen, Germany
    Pediatr Res 57:412-8. 2005
    ....
  9. doi request reprint Profiling oestrogens and testosterone in human urine by stable isotope dilution/benchtop gas chromatography-mass spectrometry
    Philipp Hoffmann
    Department of General Pediatrics and Neonatology, Center of Child and Adolescent Medicine, Justus Liebig University, Giessen, Germany
    Steroids 75:1067-74. 2010
    ..Chromatography showed clear peak shapes for the components analysed. In summary, we describe a practical, sensitive and specific ID/GC-MS assay capable of profiling the above-mentioned steroids in human urine from childhood onwards...
  10. ncbi request reprint Persistent high activity of the fetal adrenal cortex in preterm infants: is there a clinical significance?
    Matthias Heckmann
    Department of General Pediatrics and Neonatology, Center of Child and Adolescent Medicine, Justus Liebig University, Giessen, Germany
    J Pediatr Endocrinol Metab 19:1303-12. 2006
    ..Dehydroepiandrosterone sulphate can block the synthesis of surfactant in vitro. The incidence of pulmonary disease is higher in male than in female preterm infants...
  11. ncbi request reprint Cortisol production rates in preterm infants in relation to growth and illness: a noninvasive prospective study using gas chromatography-mass spectrometry
    Matthias Heckmann
    Department of General Pediatrics and Neonatology, Justus Liebig University, 35385 Giessen, Germany
    J Clin Endocrinol Metab 90:5737-42. 2005
    ..Whereas intrauterine growth and maturation depend on low cortisol levels, an adrenal stress response postnatally is thought to be mandatory in preterm infants...
  12. doi request reprint Ovarian tumor in a 12-year old female with severe hypothyroidism: A case of Van Wyk and Grumbach syndrome
    Andrea Hunold
    Department of Pediatric Hematology and Oncology, Center of Child and Adolescent, Medicine, Justus Liebig University Giessen, Giessen, Germany
    Pediatr Blood Cancer 52:677-9. 2009
    ..Although the Van Wyk and Grumbach syndrome is rare, it is crucial to rule it out in order to avoid unnecessary ovarian surgery when thyroid replacement is completely sufficient...
  13. ncbi request reprint The subtle signs of Wolfram (DIDMOAD) syndrome: not all juvenile diabetes is type 1 diabetes
    Claudia Boettcher
    Department of General Pediatrics and Neonatology, Center of Child and Adolescent Medicine, Justus Liebig University, Giessen, Germany
    J Pediatr Endocrinol Metab 24:71-4. 2011
    ....
  14. doi request reprint Profiling of bile acids in bovine follicular fluid by fused-core-LC-MS/MS
    A Sánchez-Guijo
    Steroid Research and Mass Spectrometry Unit, Division of Pediatric Endocrinology and Diabetology, Center of Child and Adolescent Medicine, Justus Liebig University, 35392 Giessen, Germany Electronic address
    J Steroid Biochem Mol Biol 162:117-25. 2016
    ..The highest concentration in CA, DCA or CDCA was always detected in FF stemming from follicles of 6-8mm. To our knowledge, this is the first report in which BAs subspecies have been detected and quantified in bovine follicular fluid. ..
  15. doi request reprint Transport of the placental estriol precursor 16α-hydroxy-dehydroepiandrosterone sulfate (16α-OH-DHEAS) by stably transfected OAT4-, SOAT-, and NTCP-HEK293 cells
    H Schweigmann
    Institute of Pharmacology and Toxicology, Justus Liebig University of Giessen, 35392 Giessen, Germany
    J Steroid Biochem Mol Biol 143:259-65. 2014
    ..At least SOAT and OAT4 seem to play a functional role for the placental estriol synthesis as both are expressed in the syncytiotrophoblast of human placenta...
  16. doi request reprint Androgen synthesis in patients with congenital adrenal hyperplasia due to 21-hydroxylase deficiency
    C Kamrath
    Division of Pediatric Endocrinology and Diabetology, Center of Child and Adolescent Medicine, Justus Liebig University, Giessen, Germany
    Horm Metab Res 45:86-91. 2013
    ....
  17. doi request reprint Clinical and metabolic findings in a 6-year-old boy with a Leydig cell tumour
    S Lignitz
    Department of Pediatrics, Johannes Gutenberg University Medical School, Mainz, Germany
    Acta Paediatr 100:e280-2. 2011
    ..To analyse the urinary steroid metabolome in a boy who had true precocious puberty after a Leydig cell tumour...
  18. ncbi request reprint Determination of 17-hydroxyprogesterone in plasma by stable isotope dilution/benchtop liquid chromatography-tandem mass spectrometry
    S A Wudy
    Steroid Laboratory, Department of Pediatrics, University of Ulm, Germany
    Horm Res 53:68-71. 2000
    ..We expect that benchtop tandem mass spectrometry will open new avenues in clinical steroid analysis...
  19. doi request reprint Free and sulfated steroids secretion in postpubertal boars (Sus scrofa domestica)
    G Schuler
    Veterinary Clinic for ObstetricsGynecology and Andrology of Large and Small Animals, Faculty of Veterinary MedicineUnit of Biomathematics and Data ProcessingSteroid Research and Mass Spectrometry UnitCenter of Child and Adolescent Medicine, Justus Liebig University, Frankfurter Strasse 106, D 35392 Giessen, Germany
    Reproduction 148:303-14. 2014
    ..Moreover, a substantial use of sulfated steroids in porcine testicular steroidogenesis would assign a crucial regulatory role to steroid sulfatase, which is highly expressed in Leydig cells...
  20. doi request reprint Reduced prevalence of obesity in children with primary fructose malabsorption: a multicentre, retrospective cohort study
    S C Disse
    Department of Pediatrics, Justus Liebig University Giessen, Giessen, Germany
    Pediatr Obes 8:255-8. 2013
    ..Studies in animals and in man have demonstrated that excessive consumption of fructose can cause all components of the metabolic syndrome...
  21. ncbi request reprint 17Beta-estradiol and progesterone supplementation in extremely low-birth-weight infants
    A Trotter
    Section of Neonatology and Pediatric Critical Care Medicine, Children s Hospital, University of Ulm, Germany
    Pediatr Res 45:489-93. 1999
    ..The infants in this study are enrolled in a randomized, controlled pilot study to evaluate the potential benefits of E2 and P supplementation...
  22. ncbi request reprint A male twin infant with skull deformity and elevated neonatal 17-hydroxyprogesterone: a prismatic case of P450 oxidoreductase deficiency
    Stefan A Wudy
    Steroid Research Unit, Center of Child and Adolescent Medicine, Justus Liebig University, Giessen, Germany
    Endocr Res 30:957-64. 2004
    ..Clinical features may include an ABS phenotype, ambiguous genitalia (virilization in girls, feminization in boys), and glucocorticoid deficiency. If required, hydrocortisone replacement should be provided...
  23. ncbi request reprint Urinary markers of adrenarche: reference values in healthy subjects, aged 3-18 years
    Thomas Remer
    Department of Nutrition and Health, Research Institute of Child Nutrition, Heinstück 11, 44225 Dortmund, Germany
    J Clin Endocrinol Metab 90:2015-21. 2005
    ..Our data may also be used as reference data for the diagnosis of steroid-related disorders...
  24. ncbi request reprint Reduced 11beta-hydroxysteroid dehydrogenase type 1 activity in obese boys
    Susanna Wiegand
    Paediatric Endocrinology, Campus Virchow, Charite University Medicine Berlin, 13353, Berlin, Germany
    Eur J Endocrinol 157:319-24. 2007
    ..Altered 11beta-HSD1 may cause tissue-specific Cushing's syndrome with central obesity and impaired glucose homeostasis...
  25. doi request reprint Metabolic evidence for impaired 17alpha-hydroxylase activity in a kindred bearing the E305G mutation for isolate 17,20-lyase activity
    Dov Tiosano
    Pediatric Endocrinology, Metabolic Biochemistry Laboratory and Genetics Institute, Rambam Medical Center, Meyer Children s Hospital, Technion Israel Institute of Technology, 9602 Haifa, Israel
    Eur J Endocrinol 158:385-92. 2008
    ..Expression studies suggested intact 17alpha-hydroxylase activity which was at odds with subnormal tetracosactrin stimulated cortisol in the patients...
  26. ncbi request reprint Adrenal steroid hormones and metaphyseal bone in children
    Thomas Remer
    Department of Nutrition and Health, Research Institute of Child Nutrition, Dortmund, Germany
    Horm Res 62:221-6. 2004
    ..Therefore, we studied potential hormonal influences in children before the appearance of pubic hair (onset of pubarche)...
  27. ncbi request reprint Adrenarche and bone modeling and remodeling at the proximal radius: weak androgens make stronger cortical bone in healthy children
    Thomas Remer
    Department of Nutrition and Health, Research Institute of Child Nutrition, Dortmund, Germany
    J Bone Miner Res 18:1539-46. 2003
    ..Positive adrenarchal effects on radial diaphyseal bone were observed. Obviously, adrenarche is one determinant of bone mineral status in children...
  28. ncbi request reprint Beyond adrenal and ovarian androgen generation: Increased peripheral 5 alpha-reductase activity in women with polycystic ovary syndrome
    Martin Fassnacht
    Department of Medicine, Endocrine and Diabetes Unit, University of Wurzburg, 97080 Wurzburg, Germany
    J Clin Endocrinol Metab 88:2760-6. 2003
    ..05). Taken together, these data indicate enhanced peripheral 5 alpha-reductase activity in PCOS. Thus, not only ovary and adrenal, but also liver and peripheral target tissues, significantly contribute to steroid alterations in PCOS...
  29. ncbi request reprint Glimepiride treatment and IGF-I in adolescents with type 1 diabetes: a prospective, randomized, double-blind, placebo-controlled study
    Stefan A Wudy
    Diabetes Care 26:1312-3. 2003
  30. ncbi request reprint Secondary adrenal failure due to long-term treatment with flunitrazepam
    Karsten Müssig
    Clin Endocrinol (Oxf) 65:549-50. 2006
  31. ncbi request reprint 17alpha-hydroxylase/17,20-lyase deficiency caused by a novel homozygous mutation (Y27Stop) in the cytochrome CYP17 gene
    Karsten Müssig
    Department of Endocrinology, Metabolism, and Pathobiochemistry, University Hospital of Internal Medicine, University of Tubingen, D 72076 Tubingen, Germany
    J Clin Endocrinol Metab 90:4362-5. 2005
    ..We report on a case of complete 17alpha-hydroxylase/17,20-lyase deficiency due to a novel homozygous mutation of CYP17...
  32. ncbi request reprint Exaggerated adrenarche and altered cortisol metabolism in Type 1 diabetic children
    Thomas Remer
    Department of Nutrition and Health, Research Institute of Child Nutrition, Dortmund, Germany
    Steroids 71:591-8. 2006
    ..Whether the elevated urinary 6beta-hydroxycortisol and cortisone excretion, observed in the patients, are also present in other forms of hypercortisolism and may thus serve as non-invasive clinical stress markers deserves further study...
  33. pmc Homozygous mutation G539R in the gene for P450 oxidoreductase in a family previously diagnosed as having 17,20-lyase deficiency
    Eli Hershkovitz
    Pediatric Endocrinology and Metabolic Unit, Soroka University Medical Center, P O Box 151, Beer Sheva 84101, Israel
    J Clin Endocrinol Metab 93:3584-8. 2008
    ..Most patients with 17,20-lyase deficiency have mutations in the domain of P450c17 that interact with the electron-donating redox partner, P450 oxidoreductase (POR)...
  34. ncbi request reprint Glucocorticoid measurements in health and disease--metabolic implications and the potential of 24-h urine analyses
    Thomas Remer
    Forschungsinstitut für Kinderernährung, Research Institute of Child Nutrition, Department of Nutrition and Health, Heinstück 11, 44225 Dortmund, Gemany
    Mini Rev Med Chem 8:153-70. 2008
    ..A special emphasis is placed on the potential of 24-h urine analyses to assess not only glucocorticoid secretion, but also functional glucocorticoid activity...
  35. doi request reprint Aldosterone synthase deficiency caused by a homozygous L451F mutation in the CYP11B2 gene
    Huy Hoang Nguyen
    Department of Biochemistry, Saarland University, D 66041 Saarbrucken, Germany
    Mol Genet Metab 93:458-67. 2008
    ..Thus, by combining highly sensitive hormone detection in a spot urine sample with expression of the mutated cDNA in cell culture the phenotype of the patient can be correlated with a particular molecular defect...
  36. ncbi request reprint Evidence for involvement of the vitamin D receptor gene in idiopathic short stature via a genome-wide linkage study and subsequent association studies
    Astrid Dempfle
    Institute of Medical Biometry and Epidemiology, Philipps University Marburg, Germany
    Hum Mol Genet 15:2772-83. 2006
    ..In conclusion, we identified significant linkage, which might be due to a functional SNP in the vitamin D receptor (VDR) gene and could be responsible for up to 34% of ISS cases in the population...
  37. ncbi request reprint Gonadectomy in mice of the inbred strain CE/J induces proliferation of sub-capsular adrenal cells expressing gonadal marker genes
    Inga K Johnsen
    Division of Endocrinology and Metabolism, Department of Internal Medicine II, Klinikum der Albert Ludwigs Universität, D 79106 Freiburg, Germany
    J Endocrinol 190:47-57. 2006
    ..The distinct expression pattern of this cell population is consistent with a defect in the differentiation of these cells into a cell population with functional properties that otherwise define a gonadal endocrine phenotype...