Alexander Steinkasserer

Summary

Affiliation: University of Erlangen-Nuremberg
Country: Germany

Publications

  1. doi request reprint Infection of dendritic cells with herpes simplex virus type 1 induces rapid degradation of CYTIP, thereby modulating adhesion and migration
    Alexandros A Theodoridis
    Department of Immune Modulation at the Department of Dermatology, University Hospital Erlangen, Erlangen, Germany
    Blood 118:107-15. 2011
  2. doi request reprint Small interfering RNA (siRNA) delivery into murine bone marrow-derived dendritic cells by electroporation
    Jonathan Jantsch
    Department of Nephrology and Hypertension, University Hospital Erlangen, Friedrich Alexander University of Erlangen Nuremberg, Krankenhausstrasse 12, Erlangen, Germany
    J Immunol Methods 337:71-7. 2008
  3. doi request reprint Human dendritic cells efficiently phagocytose adenoviral oncolysate but require additional stimulation to mature
    Stephan Schierer
    Department of Dermatology, University Hospital Erlangen, Erlangen, Germany
    Int J Cancer 130:1682-94. 2012
  4. doi request reprint Denileukin diftitox (ONTAK) induces a tolerogenic phenotype in dendritic cells and stimulates survival of resting Treg
    Andreas S Baur
    Department of Dermatology, University Hospital Erlangen, Erlangen, Germany
    Blood 122:2185-94. 2013
  5. doi request reprint Generation of human dendritic cells that simultaneously secrete IL-12 and have migratory capacity by adenoviral gene transfer of hCD40L in combination with IFN-gamma
    Ilka Knippertz
    Department of Dermatology, University Hospital Erlangen, Erlangen, Germany
    J Immunother 32:524-38. 2009
  6. doi request reprint Modulation of murine bone marrow-derived dendritic cells and B-cells by MCS-18 a natural product isolated from Helleborus purpurascens
    Leonie Littmann
    Department of Dermatology, University Hospital Erlangen, Hartmannstrasse 14, D 91052 Erlangen, Germany
    Immunobiology 213:871-8. 2008
  7. doi request reprint Mild hyperthermia enhances human monocyte-derived dendritic cell functions and offers potential for applications in vaccination strategies
    Ilka Knippertz
    Department of Immune Modulation at the Department of Dermatology, University Hospital Erlangen, Hartmannstrasse 14, Erlangen, Germany
    Int J Hyperthermia 27:591-603. 2011
  8. ncbi request reprint CD83 knockdown in monocyte-derived dendritic cells by small interfering RNA leads to a diminished T cell stimulation
    Alexander T Prechtel
    Department of Dermatology, University Hospital Erlangen, Hartmannstrasse 14, Erlangen, Germany
    J Immunol 178:5454-64. 2007
  9. doi request reprint MCS-18, a novel natural plant product prevents autoimmune diabetes
    Christian Seifarth
    Department of Internal Medicine I, University Hospital Erlangen, Ulmenweg 18, D 91052 Erlangen, Germany
    Immunol Lett 139:58-67. 2011
  10. doi request reprint HSV-1 upregulates the ARE-binding protein tristetraprolin in a STAT1- and p38-dependent manner in mature dendritic cells
    Mirko Kummer
    Department of Dermatology, University Hospital Erlangen, Germany
    Immunobiology 214:852-60. 2009

Collaborators

Detail Information

Publications48

  1. doi request reprint Infection of dendritic cells with herpes simplex virus type 1 induces rapid degradation of CYTIP, thereby modulating adhesion and migration
    Alexandros A Theodoridis
    Department of Immune Modulation at the Department of Dermatology, University Hospital Erlangen, Erlangen, Germany
    Blood 118:107-15. 2011
    ..Thus, we propose a new mechanism of pathogen-interference with central aspects of leukocyte biology...
  2. doi request reprint Small interfering RNA (siRNA) delivery into murine bone marrow-derived dendritic cells by electroporation
    Jonathan Jantsch
    Department of Nephrology and Hypertension, University Hospital Erlangen, Friedrich Alexander University of Erlangen Nuremberg, Krankenhausstrasse 12, Erlangen, Germany
    J Immunol Methods 337:71-7. 2008
    ..This new approach is superior to lipofection regarding detrimental effects of lipid-based transfection agents on BM-DC immunobiology...
  3. doi request reprint Human dendritic cells efficiently phagocytose adenoviral oncolysate but require additional stimulation to mature
    Stephan Schierer
    Department of Dermatology, University Hospital Erlangen, Erlangen, Germany
    Int J Cancer 130:1682-94. 2012
    ..We conclude that triggering and shaping DC-induced antitumor immunity by oncolytic adenoviruses "armed" with immunostimulatory genes holds promise for improving the therapeutic outcome of viral oncolysis in patients...
  4. doi request reprint Denileukin diftitox (ONTAK) induces a tolerogenic phenotype in dendritic cells and stimulates survival of resting Treg
    Andreas S Baur
    Department of Dermatology, University Hospital Erlangen, Erlangen, Germany
    Blood 122:2185-94. 2013
    ..We conclude that DD exerts functions beyond CD25(+) cell killing that may affect their clinical use and could be tested for novel indications...
  5. doi request reprint Generation of human dendritic cells that simultaneously secrete IL-12 and have migratory capacity by adenoviral gene transfer of hCD40L in combination with IFN-gamma
    Ilka Knippertz
    Department of Dermatology, University Hospital Erlangen, Erlangen, Germany
    J Immunother 32:524-38. 2009
    ..This strategy to generate DCs that exert both migration capacity and prolonged IL-12p70 secretion after intracellular CD40L expression and IFN-gamma treatment has the potential to further improve current DC vaccination protocols...
  6. doi request reprint Modulation of murine bone marrow-derived dendritic cells and B-cells by MCS-18 a natural product isolated from Helleborus purpurascens
    Leonie Littmann
    Department of Dermatology, University Hospital Erlangen, Hartmannstrasse 14, D 91052 Erlangen, Germany
    Immunobiology 213:871-8. 2008
    ..Interestingly MCS-18 inhibited the expression of important DC-specific molecules and lead to an impaired T-cell stimulation capacity. In addition, MCS-18 also reduced B-cell proliferation and immunoglobulin production...
  7. doi request reprint Mild hyperthermia enhances human monocyte-derived dendritic cell functions and offers potential for applications in vaccination strategies
    Ilka Knippertz
    Department of Immune Modulation at the Department of Dermatology, University Hospital Erlangen, Hartmannstrasse 14, Erlangen, Germany
    Int J Hyperthermia 27:591-603. 2011
    ..Thus, our strategy of heat treatment of DCs offers a promising means to improve DC functions during immune activation which, as a physical method, facilitates straight-forward applications in clinical DC vaccination protocols...
  8. ncbi request reprint CD83 knockdown in monocyte-derived dendritic cells by small interfering RNA leads to a diminished T cell stimulation
    Alexander T Prechtel
    Department of Dermatology, University Hospital Erlangen, Hartmannstrasse 14, Erlangen, Germany
    J Immunol 178:5454-64. 2007
    ..These results were obtained for the first time without the use of agents that might cause unwanted side effects, such as low m.w. inhibitors or viruses. Therefore, this method presents a suitable way to influence DC biology...
  9. doi request reprint MCS-18, a novel natural plant product prevents autoimmune diabetes
    Christian Seifarth
    Department of Internal Medicine I, University Hospital Erlangen, Ulmenweg 18, D 91052 Erlangen, Germany
    Immunol Lett 139:58-67. 2011
    ..These studies show that MCS-18 exerts an efficient immunosuppressive activity with remarkable potential for the therapy of diseases characterized by pathological over-activation of the immune system...
  10. doi request reprint HSV-1 upregulates the ARE-binding protein tristetraprolin in a STAT1- and p38-dependent manner in mature dendritic cells
    Mirko Kummer
    Department of Dermatology, University Hospital Erlangen, Germany
    Immunobiology 214:852-60. 2009
    ..In summary, these data underline that HSV-1 induces TTP transcription in order to reduce TNF levels generated by infected mature dendritic cell...
  11. doi request reprint Eukaryotic expression of functionally active recombinant soluble CD83 from HEK 293T cells
    Christine Staab
    Department of Dermatology, University Hospital Erlangen, Erlangen, Germany
    Immunobiology 215:849-54. 2010
    ..coli. Thus, a new protocol for efficient eukaryotic expression and purification of sCD83 was established, which might have several advantages compared to prokaryotic expression systems...
  12. doi request reprint Herpes simplex virus type I infection of mature dendritic cells leads to reduced LMP7-mRNA-expression levels
    Jutta Eisemann
    Department of Dermatology, University Hospital Erlangen, Germany
    Immunobiology 214:861-7. 2009
    ....
  13. pmc Herpes simplex virus type 1 induces CD83 degradation in mature dendritic cells with immediate-early kinetics via the cellular proteasome
    Mirko Kummer
    Department of Dermatology, University Hospital Erlangen, Hartmannstrasse 14, D 91052 Erlangen, Germany
    J Virol 81:6326-38. 2007
    ....
  14. doi request reprint Herpes simplex virus type I (HSV-1) replicates in mature dendritic cells but can only be transferred in a cell-cell contact-dependent manner
    Andreas Goldwich
    Department of Immunemodulation, University Hospital Erlangen, Erlangen, Germany
    J Leukoc Biol 89:973-9. 2011
    ..Finally, we provide evidence that the viral gE is involved in the transfer of infectious virus from mMDDCs to other permissive cells...
  15. doi request reprint Minor role of bystander tolerance to fetal calf serum in a peptide-specific dendritic cell vaccine model against autoimmunity: comparison with serum-free cultures
    Susanne Röner
    Department of Dermatology, University Hospital Erlangen, Erlangen, Germany
    J Immunother 31:656-64. 2008
    ..Together, our data show that presentation of bystander antigens by peptide-specific DC vaccinations may have little influence on T-cell responses in vivo if the bystander antigen cannot be recalled by specific T cells...
  16. doi request reprint Herpes simplex virus type 1 ICP0 induces CD83 degradation in mature dendritic cells independent of its E3 ubiquitin ligase function
    Christiane S Heilingloh
    Department of Immune Modulation, University Hospital Erlangen, Hartmannstrasse 14, D 91052 Erlangen, Germany
    J Gen Virol 95:1366-75. 2014
    ..Taken together, our data provide strong evidence that ICP0 alone induces CD83 degradation independent of its E3 ubiquitin ligase function and of the ubiquitin machinery...
  17. pmc Multiple interferon regulatory factor and NF-κB sites cooperate in mediating cell-type- and maturation-specific activation of the human CD83 promoter in dendritic cells
    Marcello F Stein
    Department of Dermatology, University Hospital Erlangen, Erlangen, Germany
    Mol Cell Biol 33:1331-44. 2013
    ....
  18. pmc Podocytes are nonhematopoietic professional antigen-presenting cells
    Andreas Goldwich
    Department of Immunemodulation at the Dermatology, University Hospital Erlangen, Erlangen, Germany
    J Am Soc Nephrol 24:906-16. 2013
    ..In conclusion, podocytes participate in the antigen-specific activation of adaptive immune responses, providing a potential target for immunotherapies of inflammatory kidney diseases and transplant rejection...
  19. doi request reprint Soluble human CD83 ameliorates lupus in NZB/W F1 mice
    Charlotte Starke
    Department of Internal Medicine 3 and Institute of Clinical Immunology, Nikolaus Fiebiger Center, University of Erlangen Nuremberg, Erlangen, Germany Department of Internal Medicine 3, University of Technology, Dresden, Germany
    Immunobiology 218:1411-5. 2013
    ....
  20. doi request reprint The IL-2 diphtheria toxin fusion protein denileukin diftitox modulates the onset of diabetes in female nonobese diabetic animals in a time-dependent manner and breaks tolerance in male nonobese diabetic animals
    Elisabeth Zinser
    Department of Immune Modulation at the Department of Dermatology, University Hospital Erlangen, D 91052 Erlangen, Germany
    J Immunol 189:1173-81. 2012
    ..These finding should be considered when denileukin diftitox is used for the treatment of patients suffering from tumors and/or autoimmune disorders...
  21. doi request reprint Impairment of podocyte function by diphtheria toxin--a new reversible proteinuria model in mice
    Andreas Goldwich
    Department of Immunemodulation at the Dermatology, University Hospital Erlangen, Erlangen, Germany
    Lab Invest 92:1674-85. 2012
    ..We suggest DTx-induced transient kidney dysfunction as a new reversible model of experimental podocyte injury, which could be used as an additional approach to complement studies in human...
  22. doi request reprint Inhibition of the proteasome influences murine and human dendritic cell development in vitro and in vivo
    Elisabeth Zinser
    Department of Dermatology, University Hospital Erlangen, Germany
    Immunobiology 214:843-51. 2009
    ..Thus, inhibition of the proteasome interferes with DC maturation and subsequently with DC-mediated T cell stimulation events...
  23. ncbi request reprint Hypoxia and hypoxia-inducible factor-1 alpha modulate lipopolysaccharide-induced dendritic cell activation and function
    Jonathan Jantsch
    Department of Nephrology and Hypertension, University Hospital Erlangen, Friedrich Alexander University of Erlangen Nuremberg, Erlangen, Germany
    J Immunol 180:4697-705. 2008
    ..Alltogether, our data indicate that HIF-1alpha and hypoxia play a crucial role for DC activation in inflammatory states, which is highly dependent on glycolysis even in the presence of oxygen...
  24. ncbi request reprint Infection of human dendritic cells with herpes simplex virus type 1 dramatically diminishes the mRNA levels of the prostaglandin E(2) receptors EP2 and EP4
    Alexandros A Theodoridis
    Department of Dermatology, University Hospital Erlangen, Erlangen, Hartmannstrasse 14, 91052, Erlangen, Germany
    Immunobiology 212:827-38. 2007
    ..Additional real-time PCR and migration assays with a Deltavhs mutant virus lacking the virion host shutoff (vhs) gene implicate a vhs independent mechanism. Therefore, our results suggest a novel immune evasion strategy for HSV-1...
  25. ncbi request reprint MCS-18, a novel natural product isolated from Helleborus purpurascens, inhibits dendritic cell activation and prevents autoimmunity as shown in vivo using the EAE model
    Brigitte Horstmann
    Department of Dermatology, University Hospital Erlangen, Hartmannstrasse 14, D 91052, Erlangen, Germany
    Immunobiology 212:839-53. 2007
    ....
  26. pmc L Particles Transmit Viral Proteins from Herpes Simplex Virus 1-Infected Mature Dendritic Cells to Uninfected Bystander Cells, Inducing CD83 Downmodulation
    Christiane S Heilingloh
    Department of Immune Modulation, University Hospital Erlangen, Erlangen, Germany
    J Virol 89:11046-55. 2015
    ....
  27. doi request reprint CD83 and GRASP55 interact in human dendritic cells
    Marcello F Stein
    Department of Immune Modulation, Universitatsklinikum Erlangen, Erlangen, Germany
    Biochem Biophys Res Commun 459:42-8. 2015
    ..Here we show for the first time that GRASP55 interacts with CD83 shortly after induction of DC maturation and that this interaction plays a role in CD83 glycosylation as well as in surface expression of CD83 on DCs. ..
  28. ncbi request reprint Infection of mature dendritic cells with herpes simplex virus type 1 interferes with the interferon signaling pathway
    Jutta Eisemann
    Department of Dermatology, University Hospital Erlangen, Hartmannstrasse 14, D 91052, Erlangen, Germany
    Immunobiology 212:877-86. 2007
    ..In conclusion, we show that HSV-1 blocks IFN-gamma signaling in mDCs, which requires viral gene expression and involves the viral protein vhs...
  29. ncbi request reprint CNI-1493 mediated suppression of dendritic cell activation in vitro and in vivo
    Elisabeth Zinser
    Department of Dermatology, University Hospital Erlangen, Hartmannstrasse 14, D 91052 Erlangen, Germany
    Immunobiology 209:89-97. 2004
    ..Since DC are the only antigen-presenting cells (APC) known today to be able to prime naive T cells, the findings reported herein are highly relevant for the therapeutic application of CNI-1493...
  30. ncbi request reprint Modulation of viability and maturation of human monocyte-derived dendritic cells by oncolytic adenoviruses
    Stephan Schierer
    Department of Dermatology, University Hospital Erlangen, Erlangen, Germany
    Int J Cancer 122:219-29. 2008
    ..Thus additional signals are required for optimal immune activation. These could be delivered, for example, by inserting immunoregulatory transgenes into the oncolytic adenovirus genome...
  31. doi request reprint MCS-18, a natural product isolated from Helleborus purpurascens, inhibits maturation of dendritic cells in ApoE-deficient mice and prevents early atherosclerosis progression
    Barbara Dietel
    Department of Cardiology and Angiology, University Hospital Erlangen, Erlangen, Germany Electronic address
    Atherosclerosis 235:263-72. 2014
    ..The aim of this study was to investigate the immune modulatory effects of the natural substance MCS-18, an inhibitor of DC maturation, regarding the progression of atherosclerosis in ApoE-deficient mice...
  32. doi request reprint Electroporation of siRNA into mouse bone marrow-derived macrophages and dendritic cells
    Isabel Siegert
    Mikrobiologisches Institut Klinische Mikrobiologie, Immunologie und Hygiene, Universitatsklinikum Erlangen, Friedrich Alexander Universitat Erlangen Nurnberg, Erlangen, Germany
    Methods Mol Biol 1121:111-9. 2014
    ..Factors that influence the transfection and knockdown rate will be highlighted. ..
  33. ncbi request reprint Dendritic cell differentiation state and their interaction with NKT cells determine Th1/Th2 differentiation in the murine model of Leishmania major infection
    Carsten Wiethe
    Department of Dermatology, University Hospital Erlangen, Erlangen, Germany
    J Immunol 180:4371-81. 2008
    ..These results have generic implications for the understanding of DC-driven Th cell responses and the development of improved DC vaccines against leishmaniasis...
  34. ncbi request reprint CD83: an update on functions and prospects of the maturation marker of dendritic cells
    Alexander T Prechtel
    Department of Dermatology, University Hospital Erlangen, Hartmannstrasse 14, 91052, Erlangen, Germany
    Arch Dermatol Res 299:59-69. 2007
    ..Moreover, some viruses prevent recognition by the host's immune system by specifically targeting CD83 surface expression...
  35. ncbi request reprint Small interfering RNA (siRNA) delivery into monocyte-derived dendritic cells by electroporation
    Alexander T Prechtel
    Department of Dermatology, University Hospital Erlangen, Hartmannstrasse 14, D 91052 Erlangen, Germany
    J Immunol Methods 311:139-52. 2006
    ..This new approach represents an advantage especially in the light of clinical trials...
  36. pmc Prevention and treatment of experimental autoimmune encephalomyelitis by soluble CD83
    Elisabeth Zinser
    Department of Dermatology, Hartmannstasse 14, University Hospital Erlangen, 91052 Erlangen, Germany
    J Exp Med 200:345-51. 2004
    ..In summary, this work strongly supports an immunosuppressive role of soluble CD83, thereby indicating its therapeutic potential in the regulation of immune disorders in vivo...
  37. ncbi request reprint Role of CD83 in the immunomodulation of dendritic cells
    Matthias Lechmann
    Department of Dermatology, University of Erlangen Nurnberg, Erlangen, Germany
    Int Arch Allergy Immunol 129:113-8. 2002
    ..Additional studies elucidated immunostimulatory as well as regulatory effects of the CD83 molecule. Furthermore, CD83-/- knockout mice revealed a block in CD4+ T cell generation, a new possible immunomodulatory function of CD83...
  38. ncbi request reprint Interleukin-10 influences the expression of MRP8 and MRP14 in human dendritic cells
    Anita Kumar
    Department of Dermatology, University Hospital Erlangen, Erlangen, Germany
    Int Arch Allergy Immunol 132:40-7. 2003
    ..We investigated the differential gene expression of human dendritic cells (DC) under the influence of the cytokine interleukin-10 (IL-10)...
  39. ncbi request reprint HMG-CoA reductase inhibitors suppress maturation of human dendritic cells: new implications for atherosclerosis
    Atilla Yilmaz
    Medical Clinic II, University Erlangen Nuremberg, Ulmenweg 18, 91054, Erlangen, Germany
    Atherosclerosis 172:85-93. 2004
    ..Therefore, the use of statins as immunomodulators might also provide a new therapeutic approach to other immunological disorders...
  40. ncbi request reprint CD83 is a dimer: Comparative analysis of monomeric and dimeric isoforms
    Matthias Lechmann
    University Hospital Erlangen, Department of Dermatology, Hartmannstr 14, D 91052 Erlangen, Germany
    Biochem Biophys Res Commun 329:132-9. 2005
    ..Furthermore, the functional activity of the mutant protein was investigated using MLR assays and we could show that the mutant soluble CD83 protein inhibited DC-mediated allogeneic T-cell stimulation in vitro...
  41. doi request reprint Third-Kind Encounters in Biomedicine: Immunology Meets Mathematics and Informatics to Become Quantitative and Predictive
    Martin Eberhardt
    Laboratory of Systems Tumor Immunology, Department of Dermatology, University Hospital Erlangen and Friedrich Alexander University Erlangen Nürnberg, Erlangen, Germany
    Methods Mol Biol 1386:135-79. 2016
    ..In this chapter, we support the idea that the analysis of the immune system demands the use of systems-level approaches to ensure the success in the search for more effective and personalized immune-based therapies. ..
  42. doi request reprint Murine CD83-positive T cells mediate suppressor functions in vitro and in vivo
    Simon Kreiser
    Department of Immune Modulation at the Department of Dermatology, University Hospital Erlangen, Erlangen D 91052, Germany
    Immunobiology 220:270-9. 2015
    ..Thus, from these studies we conclude that CD83(+) T cells share important features with regulatory T cells, identifying CD83 as a novel lineage marker to discriminate between different T cell populations. ..
  43. ncbi request reprint The soluble form of CD83 dramatically changes the cytoskeleton of dendritic cells
    Nicole Kotzor
    Department of Dermatology, University Hospital Erlangen, Hartmannstr 14, D 91052 Erlangen, Germany
    Immunobiology 209:129-40. 2004
    ..Furthermore, soluble CD83-treated cells were completely inhibited in their ability to form clusters with T cells, an absolute prerequisite in order to stimulate T cells...
  44. ncbi request reprint CD83 on dendritic cells: more than just a marker for maturation
    Matthias Lechmann
    Dept of Dermatology, University of Erlangen Nurnberg, Hartmannstrasse 14, D 91052, Erlangen, Germany
    Trends Immunol 23:273-5. 2002
    ..Recently, we have shown that the soluble extracellular CD83 domain inhibits DC-mediated T-cell proliferation, representing the first report describing a functional role for CD83...
  45. ncbi request reprint The cyclin-dependent kinase inhibitors p27Kip1 and p21Cip1 are not essential in T cell anergy
    Berlinda Verdoodt
    Department of Dermatology, University of Erlangen, Erlangen, Germany
    Eur J Immunol 33:3154-63. 2003
    ..These data indicate that other cell cycle regulators contribute to the cell cycle arrest of anergic T cells, as neither Cdk inhibitor is required for the induction of anergy...
  46. ncbi request reprint Differences in phenotype and function between spontaneously occurring melan-A-, tyrosinase- and influenza matrix peptide-specific CTL in HLA-A*0201 melanoma patients
    Christian Maczek
    Department of Dermatology, University Hospital of Erlangen Nuremberg, Erlangen, Germany
    Int J Cancer 115:450-5. 2005
    ....
  47. ncbi request reprint DNA viruses and genetic modification of dendritic cells
    Lars Jenne
    Department of Dermatology, University of Erlangen Nurnberg, Erlangen, Germany
    Arch Immunol Ther Exp (Warsz) 50:273-7. 2002
    ..Here we report that vaccinia virus (VV) as well as herpes simplex virus type 1 (HSV-1) are able to potently infect monocyte-derived DC, however, this infection leads to the inhibition of the DC-mediated T cell stimulation in vitro...
  48. ncbi request reprint Determination of the inhibitory activity and biological half-live of soluble CD83: comparison of wild type and mutant isoforms
    Elisabeth Zinser
    Department of Dermatology, University Hospital Erlangen, D 91052 Erlangen, Germany
    Immunobiology 211:449-53. 2006
    ..In addition, using the EAE-model, we were able to show that a monomeric-mutant isoform of soluble CD83 has a similar inhibitory activity in vivo when compared with a dimeric-wildtype isoform...