Alexander Pfeifer

Summary

Affiliation: University of Bonn
Country: Germany

Publications

  1. doi request reprint Pharmacological potential of RNAi--focus on miRNA
    Alexander Pfeifer
    Institute of Pharmacology and Toxicology, University of Bonn, Biomedical Center, Sigmund Freud Str 25, 53105 Bonn, Germany
    Pharmacol Ther 126:217-27. 2010
  2. pmc Lentivector-mediated RNAi efficiently suppresses prion protein and prolongs survival of scrapie-infected mice
    Alexander Pfeifer
    Institute of Pharmacology and Toxicology, University of Bonn, Bonn, Germany
    J Clin Invest 116:3204-10. 2006
  3. doi request reprint NRG4: an endocrine link between brown adipose tissue and liver
    Alexander Pfeifer
    Institute of Pharmacology and Toxicology, University of Bonn, 53127 Bonn, Germany Electronic address
    Cell Metab 21:13-4. 2015
  4. doi request reprint Brown, beige, and white: the new color code of fat and its pharmacological implications
    Alexander Pfeifer
    Institute of Pharmacology and Toxicology, Biomedical Center, University of Bonn, 53105 Bonn, Germany email
    Annu Rev Pharmacol Toxicol 55:207-27. 2015
  5. doi request reprint Regulation of metabolism by cGMP
    Alexander Pfeifer
    Institute of Pharmacology and Toxicology, Biomedical Center, University of Bonn, Germany Electronic address
    Pharmacol Ther 140:81-91. 2013
  6. doi request reprint Optimization of magnetic nanoparticle-assisted lentiviral gene transfer
    Christina Trueck
    Institute of Pharmacology and Toxicology, Biomedical Center, University of Bonn, Sigmund Freud Strasse 25, 53105 Bonn, Germany
    Pharm Res 29:1255-69. 2012
  7. doi request reprint Increased cGMP promotes healthy expansion and browning of white adipose tissue
    Michaela M Mitschke
    Institute of Pharmacology and Toxicology, University of Bonn, Bonn, Germany
    FASEB J 27:1621-30. 2013
  8. doi request reprint Identification of magnetic nanoparticles for combined positioning and lentiviral transduction of endothelial cells
    Daniela Wenzel
    Institute of Physiology I, University of Bonn, Sigmund Freud Str 25, 53127 Bonn, Germany
    Pharm Res 29:1242-54. 2012
  9. doi request reprint Analysis of cGMP signaling in adipocytes
    Katja Jennissen
    Institute of Pharmacology and Toxicology, Universitat Bonn, Bonn, Germany
    Methods Mol Biol 1020:175-92. 2013
  10. pmc Lentiviral vector mediated thymidine kinase expression in pluripotent stem cells enables removal of tumorigenic cells
    Tiong Ti Lim
    Institute of Pharmacology and Toxicology, University of Bonn, Bonn, Germany
    PLoS ONE 8:e70543. 2013

Collaborators

Detail Information

Publications49

  1. doi request reprint Pharmacological potential of RNAi--focus on miRNA
    Alexander Pfeifer
    Institute of Pharmacology and Toxicology, University of Bonn, Biomedical Center, Sigmund Freud Str 25, 53105 Bonn, Germany
    Pharmacol Ther 126:217-27. 2010
    ..Furthermore, miRNA-based diagnostics as well as therapeutic interventions are being developed for clinical applications...
  2. pmc Lentivector-mediated RNAi efficiently suppresses prion protein and prolongs survival of scrapie-infected mice
    Alexander Pfeifer
    Institute of Pharmacology and Toxicology, University of Bonn, Bonn, Germany
    J Clin Invest 116:3204-10. 2006
    ..Importantly, in highly chimeric mice, survival after scrapie infection was significantly extended. Taken together, these data suggest that lentivector-mediated RNA interference could be an approach for the treatment of prion disease...
  3. doi request reprint NRG4: an endocrine link between brown adipose tissue and liver
    Alexander Pfeifer
    Institute of Pharmacology and Toxicology, University of Bonn, 53127 Bonn, Germany Electronic address
    Cell Metab 21:13-4. 2015
    ..Brown adipose tissue (BAT) directly regulates energy homeostasis via uncoupling of mitochondrial ATP production and thermogenesis. Wang et al. (2014) now report that BAT controls liver lipogenesis via secretion of the growth factor NRG4. ..
  4. doi request reprint Brown, beige, and white: the new color code of fat and its pharmacological implications
    Alexander Pfeifer
    Institute of Pharmacology and Toxicology, Biomedical Center, University of Bonn, 53105 Bonn, Germany email
    Annu Rev Pharmacol Toxicol 55:207-27. 2015
    ..We also shine light on ongoing debates, including those about the true color of brown fat in adults, as well as on the requirements for translation of basic research on BAT into clinical medicine. ..
  5. doi request reprint Regulation of metabolism by cGMP
    Alexander Pfeifer
    Institute of Pharmacology and Toxicology, Biomedical Center, University of Bonn, Germany Electronic address
    Pharmacol Ther 140:81-91. 2013
    ..Targeting the cGMP signaling pathway could be an exciting approach for the regulation of energy expenditure and the treatment of obesity. ..
  6. doi request reprint Optimization of magnetic nanoparticle-assisted lentiviral gene transfer
    Christina Trueck
    Institute of Pharmacology and Toxicology, Biomedical Center, University of Bonn, Sigmund Freud Strasse 25, 53105 Bonn, Germany
    Pharm Res 29:1255-69. 2012
    ..We use magnetic nanoparticles (MNPs) with a ferrimagnetic core (Fe(3)O(4)) with different coatings to optimize MNP-assisted lentiviral gene transfer with focus on different endothelial cell lines...
  7. doi request reprint Increased cGMP promotes healthy expansion and browning of white adipose tissue
    Michaela M Mitschke
    Institute of Pharmacology and Toxicology, University of Bonn, Bonn, Germany
    FASEB J 27:1621-30. 2013
    ..Taken together, PKGI is a key regulator of cell size, adipokine secretion and browning of white fat depots and thus could be a valuable target in developing novel treatments for obesity...
  8. doi request reprint Identification of magnetic nanoparticles for combined positioning and lentiviral transduction of endothelial cells
    Daniela Wenzel
    Institute of Physiology I, University of Bonn, Sigmund Freud Str 25, 53127 Bonn, Germany
    Pharm Res 29:1242-54. 2012
    ..Because physical requirements for MNPs differ between these two applications we have explored whether the use of different MNPs can provide site-specific positioning combined with efficient viral transduction of endothelial cells (ECs)...
  9. doi request reprint Analysis of cGMP signaling in adipocytes
    Katja Jennissen
    Institute of Pharmacology and Toxicology, Universitat Bonn, Bonn, Germany
    Methods Mol Biol 1020:175-92. 2013
    ..Using specific differentiation protocols, the role of cGMP signaling in both brown as well as white adipocytes can be studied...
  10. pmc Lentiviral vector mediated thymidine kinase expression in pluripotent stem cells enables removal of tumorigenic cells
    Tiong Ti Lim
    Institute of Pharmacology and Toxicology, University of Bonn, Bonn, Germany
    PLoS ONE 8:e70543. 2013
    ..The study presented here gives rise to safer use of pluripotent stem cells as promising cell sources in regenerative medicine in the future. ..
  11. pmc miR-155 regulates differentiation of brown and beige adipocytes via a bistable circuit
    Yong Chen
    Institute of Pharmacology and Toxicology, University of Bonn, Sigmund Freud Strasse 25, 53127 Bonn, Germany
    Nat Commun 4:1769. 2013
    ..These data demonstrate that the bistable loop involving microRNA 155 and CCAAT/enhancer-binding protein β regulates brown lineage commitment, thereby, controlling the development of brown and beige fat cells...
  12. doi request reprint A VASP-Rac-soluble guanylyl cyclase pathway controls cGMP production in adipocytes
    Katja Jennissen
    Institute of Pharmacology and Toxicology, University of Bonn, 53105 Bonn, Germany
    Sci Signal 5:ra62. 2012
    ..Our data revealed that a VASP to Rac to sGC negative feedback loop limited cGMP production, thereby regulating adipogenesis and energy homeostasis...
  13. doi request reprint Lentivirus transgenesis
    Alexander Pfeifer
    Institute of Pharmacology and Toxicology, Biomedical Center BMZ, University of Bonn, Germany
    Methods Enzymol 477:3-15. 2010
    ..Here we present a protocol for generating transgenic animals by lentiviral transduction of early embryos as well as for analyzing viral integrants in transgenic animals...
  14. doi request reprint Changes in serum miRNAs following generalized convulsive seizures in human mesial temporal lobe epilepsy
    Rainer Surges
    Department of Epileptology, University Hospital of Bonn, Sigmund Freud Str 25, 53127, Bonn, Germany
    Biochem Biophys Res Commun 481:13-18. 2016
    ..The significant upregulation of miR-663b following BCS arising during sleep indicates potential suitability of this miRNA as a potential biomarker for seizure diagnostics...
  15. doi request reprint Lentiviral transgenesis
    Alexander Pfeifer
    Institut fur Pharmakologie und Toxikologie, Universitat Bonn, Bonn, Germany
    Methods Mol Biol 530:391-405. 2009
    ..The aim of this chapter is to cover important practical aspects of lentiviral transgenesis, including vector preparation, gene delivery into the early embryos and lentiviral RNA interference...
  16. doi request reprint Interplay between Obesity-Induced Inflammation and cGMP Signaling in White Adipose Tissue
    Abhishek Sanyal
    Institute of Pharmacology and Toxicology, University of Bonn, 53127 Bonn, Germany Bonn International Graduate School of Drug Sciences, University of Bonn, 53127 Bonn, Germany
    Cell Rep 18:225-236. 2017
    ..Additionally, TNF-α-activated JNK signaling suppresses PPARγ and aP2. Taken together, the intensity of obesity-induced inflammation dictates the amplitude of cGMP signaling dysregulation in white adipocytes through distinct pathways...
  17. pmc Highly efficient concentration of lenti- and retroviral vector preparations by membrane adsorbers and ultrafiltration
    Katrin Zimmermann
    Institute of Pharmacology and Toxicology, Biomedical Center, University of Bonn, Sigmund Freud Strasse 25, 53105 Bonn, Germany
    BMC Biotechnol 11:55. 2011
    ..The most commonly used envelope protein for pseudotyping is the glycoprotein of the Vesicular Stomatitis Virus (VSV.G), which is also essential for successful concentration using UC...
  18. pmc Stimulation of soluble guanylyl cyclase protects against obesity by recruiting brown adipose tissue
    Linda S Hoffmann
    Institute of Pharmacology and Toxicology, University Hospital Bonn, University of Bonn, Bonn D 53105, Germany
    Nat Commun 6:7235. 2015
    ..Taken together, our data suggest that sGC is a potential pharmacological target for the treatment of obesity and its comorbidities. ..
  19. doi request reprint Regulation of brown and beige fat by microRNAs
    Yong Chen
    Institute of Pharmacology and Toxicology, University Hospital Bonn, University of Bonn, 53127 Bonn, Germany
    Pharmacol Ther 170:1-7. 2017
    ..In this review, we focus on the regulation of brown and beige fat by miRNAs including the role in their differentiation and function, providing evidence for their therapeutic potential in metabolic diseases...
  20. pmc Fat tissues, the brite and the dark sides
    Yong Chen
    Institute of Pharmacology and Toxicology, University Hospital Bonn, University of Bonn, 53127, Bonn, Germany
    Pflugers Arch 468:1803-1807. 2016
    ..Thus, brown/brite adipocytes represent the "brite" side of fat and are potential targets for novel therapeutic approaches for treatment of obesity and obesity-associated diseases...
  21. pmc The Gq signalling pathway inhibits brown and beige adipose tissue
    Katarina Klepac
    Institute of Pharmacology and Toxicology, University Hospital Bonn, University of Bonn, 53127 Bonn, Germany
    Nat Commun 7:10895. 2016
    ..Thus, our data indicate that Gq signalling regulates brown/beige adipocytes and inhibition of Gq signalling may be a novel therapeutic approach to combat obesity. ..
  22. doi request reprint Non-small cell lung cancer cell survival crucially depends on functional insulin receptors
    Carolin Maria Frisch
    Institute of Pharmacology and ToxicologyUniversity of Bonn, Sigmund Freud Strase 25, 53127 Bonn, GermanyFederal Institute for Drugs and Medical Devices BfArM Kurt Georg Kiesinger Allee 3, 53175 Bonn, Germany
    Endocr Relat Cancer 22:609-21. 2015
    ..g. IL20 and tumour necrosis factor) became obvious and it turned out that these cytokines trigger apoptosis in the NSCLC cells tested. This indicates a novel role of IR in tumor cell survival via suppression of pro-apoptotic cytokines. ..
  23. doi request reprint Adenosine activates brown adipose tissue and recruits beige adipocytes via A2A receptors
    Thorsten Gnad
    Institute of Pharmacology and Toxicology, University Hospital, University of Bonn, 53127 Bonn, Germany
    Nature 516:395-9. 2014
    ..Those findings reveal new possibilities for developing novel obesity therapies...
  24. pmc Combined targeting of lentiviral vectors and positioning of transduced cells by magnetic nanoparticles
    Andreas Hofmann
    Institute for Pharmacology and Toxicology, University of Bonn, D 53115 Bonn, Germany
    Proc Natl Acad Sci U S A 106:44-9. 2009
    ..Intravascular gene targeting can be combined with positioning of the transduced cells via nanomagnetic particles, thereby combining gene- and cell-based therapies...
  25. pmc Direct visualization of cell division using high-resolution imaging of M-phase of the cell cycle
    Michael Hesse
    Institute of Physiology I, Life and Brain Center, University of Bonn, 53105 Bonn, Germany
    Nat Commun 3:1076. 2012
    ..Thus, the enhanced green fluorescent protein-anillin system enables monitoring and measurement of cell division in vivo and markedly simplifies in vitro analysis in fixed cells...
  26. doi request reprint KIAA1797/FOCAD encodes a novel focal adhesion protein with tumour suppressor function in gliomas
    Antje Brockschmidt
    Institute of Human Genetics, Biomedical Center BMZ, University of Bonn, 53105 Bonn, Germany
    Brain 135:1027-41. 2012
    ..Since KIAA1797 genetic variation has been implicated in Alzheimer's disease, our data are also relevant for neurodegeneration...
  27. doi request reprint FOXO4-dependent upregulation of superoxide dismutase-2 in response to oxidative stress is impaired in spinocerebellar ataxia type 3
    Julieta Araujo
    Department of Neurology, University of Bonn, 53105 Bonn, Germany
    Hum Mol Genet 20:2928-41. 2011
    ....
  28. ncbi request reprint Filarial Infection or Antigen Administration Improves Glucose Tolerance in Diet-Induced Obese Mice
    Afiat Berbudi
    Institute for Medical Microbiology, Immunology and Parasitology, University Hospital of Bonn, Bonn, Germany
    J Innate Immun 8:601-616. 2016
    ....
  29. pmc Exosomal microRNA miR-92a concentration in serum reflects human brown fat activity
    Yong Chen
    Institute of Pharmacology and Toxicology, University Hospital Bonn, University of Bonn, Sigmund Freud Strasse 25, 53127 Bonn, Germany
    Nat Commun 7:11420. 2016
    ..Thus, exosomal miR-92a represents a potential serum biomarker for BAT activity in mice and humans. ..
  30. doi request reprint cGMP and Brown Adipose Tissue
    Linda S Hoffmann
    Institute of Pharmacology and Toxicology, University Hospital, University of Bonn, 53127, Bonn, Germany
    Handb Exp Pharmacol 233:283-99. 2016
    ....
  31. pmc Different microRNA profiles in chronic epilepsy versus acute seizure mouse models
    Anita Kretschmann
    Institute of Pharmacology and Toxicology, University of Bonn, Sigmund Freud Str 25, 53127, Bonn, Germany
    J Mol Neurosci 55:466-79. 2015
    ..Taken together, the identified microRNAs and the pathways they modulate might represent candidates for novel molecular approaches for the treatment of patients with epilepsy...
  32. doi request reprint Protein kinase G controls brown fat cell differentiation and mitochondrial biogenesis
    Bodo Haas
    Institute for Pharmacology and Toxicology, Biomedical Center, University of Bonn, Bonn, Germany
    Sci Signal 2:ra78. 2009
    ..Thus, PKGI links NO and cGMP signaling with the RhoA-ROCK and the insulin pathways, thereby controlling induction of adipogenic and thermogenic programs during brown fat cell differentiation...
  33. doi request reprint Efficient and graded gene expression in glia and neurons of primary cerebellar cultures transduced by lentiviral vectors
    Sujeet Kumar
    Institute of Anatomy, Anatomy and Cell Biology, University of Bonn, Nussallee 10, 53125, Bonn, Germany
    Histochem Cell Biol 143:109-21. 2015
    ..A gene of interest in combination with a reporter gene can thus be expressed in a graded manner to reveal gene function in a rather short time and in a complex cellular environment. ..
  34. ncbi request reprint Engraftment of connexin 43-expressing cells prevents post-infarct arrhythmia
    Wilhelm Roell
    Institute of Physiology I, Life and Brain Center, University of Bonn, Bonn 53105, Germany
    Nature 450:819-24. 2007
    ..Thus, engraftment of Cx43-expressing myocytes has the potential to reduce life-threatening post-infarct arrhythmias through the augmentation of intercellular coupling, suggesting autologous strategies for cardiac cell-based therapy...
  35. pmc Transgenesis by lentiviral vectors: lack of gene silencing in mammalian embryonic stem cells and preimplantation embryos
    Alexander Pfeifer
    The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 99:2140-5. 2002
    ..Ability to transfer foreign genes into human ES cells has potential relevance for the development of gene and cell-based therapies...
  36. ncbi request reprint Lentiviral transgenesis--a versatile tool for basic research and gene therapy
    Alexander Pfeifer
    Department of Pharmacy Center for Drug Research, Molecular Pharmacology, Butenandtstr 5 C, Ludwig Maximilians University of Munich, Germany
    Curr Gene Ther 6:535-42. 2006
    ..Finally, lentiviral transgenic animals can be used to directly evaluate gene therapy strategies that are based on lentiviral vectors prior to their use in humans...
  37. ncbi request reprint Generation of transgenic cattle by lentiviral gene transfer into oocytes
    Andreas Hofmann
    Department of Pharmacy, Institute for Pharmacology, Center for Drug Research, Ludwig Maximilians University, Butenandstrasse 5 C, 81377 Munich, Germany
    Biol Reprod 71:405-9. 2004
    ..Taken together, after adjusting lentiviral transgenesis to cattle, unprecedented high transgenesis and expression rates were achieved...
  38. ncbi request reprint Mena and vasodilator-stimulated phosphoprotein are required for multiple actin-dependent processes that shape the vertebrate nervous system
    A Sheila Menzies
    Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 4307, USA
    J Neurosci 24:8029-38. 2004
    ..Mena-/-VASP-/- double mutants die perinatally and display defects in neurulation, craniofacial structures, and the formation of several fiber tracts in the CNS and peripheral nervous system...
  39. pmc Impaired channel targeting and retinal degeneration in mice lacking the cyclic nucleotide-gated channel subunit CNGB1
    Sabine Hüttl
    Department Pharmazie, Pharmakologie für Naturwissenschaften, Ludwig Maximilians Universitat Munchen, D 81377 Munchen, Germany
    J Neurosci 25:130-8. 2005
    ..Our results show that CNGB1 is a crucial determinant of native CNG channel targeting. As a result of the lack of rod CNG channels, CNGB1-/- mice develop a retinal degeneration that resembles human retinitis pigmentosa...
  40. pmc Efficient transgenesis in farm animals by lentiviral vectors
    Andreas Hofmann
    Department of Pharmacy, Institute for Pharmacology, Center for Drug Research, Butenandtstrasse 5 C, Ludwig Maximilians University, 81377 Munich, Germany
    EMBO Rep 4:1054-60. 2003
    ..Finally, infection of bovine oocytes after and before in vitro fertilization with LV-PGK resulted in transgene expression in 45% and 92% of the infected embryos, respectively...
  41. ncbi request reprint Lentiviral transgenesis
    Alexander Pfeifer
    Department of Pharmacy, Institute for Pharmacology, Center for Drug Research, Ludwig Maximilians University, Munich, Germany
    Transgenic Res 13:513-22. 2004
    ..In addition, lentiviral vectors can be used to transfer genes into embryonic stem cells. Due to its high efficacy and versatility, lentiviral transgenesis should have a big impact on transgenic research...
  42. ncbi request reprint Regulated lentiviral NGF gene transfer controls rescue of medial septal cholinergic neurons
    Armin Blesch
    Department of Neurosciences 0626, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Mol Ther 11:916-25. 2005
    ..These data demonstrate for the first time that NGF delivery by lentiviral gene transfer using tetracycline-regulated promoters can completely regulate neuronal rescue and protein production in the brain...
  43. ncbi request reprint Neuronal fate determinants of adult olfactory bulb neurogenesis
    Michael A Hack
    GSF National Research Center for Environment and Health, Institute for Stem Cell Research, Ingolstadter Landstr 1, D 85764 Neuherberg, Germany
    Nat Neurosci 8:865-72. 2005
    ..Together, these data explain both extrinsic and intrinsic mechanisms controlling neuronal identity in adult neurogenesis...
  44. ncbi request reprint Epigenetic regulation of lentiviral transgene vectors in a large animal model
    Andreas Hofmann
    Department of Pharmacy, Molecular Pharmacology, Center for Drug Research, Butenandtstrasse 5 C, 81377 Munich, Germany
    Mol Ther 13:59-66. 2006
    ..1. Taken together, expression of lentiviral integrants in higher mammals is regulated by epigenetic modifications. In contrast to previous expectations, DNA methylation plays an important role in lentiviral expression...
  45. ncbi request reprint Cyld inhibits tumor cell proliferation by blocking Bcl-3-dependent NF-kappaB signaling
    Ramin Massoumi
    Department of Molecular Medicine, Max Planck Institute of Biochemistry, D 82152 Martinsried, Germany
    Cell 125:665-77. 2006
    ....
  46. ncbi request reprint Evaluation of laser-assisted lentiviral transgenesis in bovine
    Sonja Ewerling
    Institute of Molecular Animal Breeding Gene Center, Ludwig Maximilians University, Munich, Germany
    Transgenic Res 15:447-54. 2006
    ..However, further optimization of the procedure is required, mainly to reduce the incidence of polyspermy after MD of oocytes and to eliminate negative effects of MD on early embryonic development...
  47. pmc Pancreas-specific RelA/p65 truncation increases susceptibility of acini to inflammation-associated cell death following cerulein pancreatitis
    Hana Algül
    Second Department of Internal Medicine, Klinikum rechts der Isar, Technical University of Munich, Munich, Germany
    J Clin Invest 117:1490-501. 2007
    ..Moreover, our data underscore the pancreas-specific role of NF-kappaB/Rel and suggest multidimensional roles of NF-kappaB/Rel in different cells and contexts during inflammation...
  48. ncbi request reprint Cyclic GMP-dependent protein kinase II plays a critical role in C-type natriuretic peptide-mediated endochondral ossification
    Takashi Miyazawa
    Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, Sakyo ku, Kyoto 606 8507, Japan
    Endocrinology 143:3604-10. 2002
    ..This study provides in vivo and in vitro genetic evidence that cGKII plays a critical role in CNP-mediated endochondral ossification...
  49. doi request reprint Adult neurogenesis requires Smad4-mediated bone morphogenic protein signaling in stem cells
    Dilek Colak
    Helmhotz Center Munich, Institute for Stem Cell Research, 85764 Neuherberg Munich, Germany
    J Neurosci 28:434-46. 2008
    ..Thus, BMP-mediated signaling via Smad4 is required to initiate neurogenesis from adult neural stem cells and suppress the alternative fate of oligodendrogliogenesis...