Philipp Korber

Summary

Affiliation: University of Munich
Country: Germany

Publications

  1. pmc The RSC chromatin remodelling enzyme has a unique role in directing the accurate positioning of nucleosomes
    Christian J Wippo
    Adolf Butenandt Institut, University of Munich, Munich, Germany
    EMBO J 30:1277-88. 2011
  2. doi request reprint In vitro reconstitution of in vivo-like nucleosome positioning on yeast DNA
    Christian J Wippo
    Molecular Biology Unit, Adolf Butenandt Institut, University of Munich, Munich, Germany
    Methods Mol Biol 833:271-87. 2012
  3. pmc The yeast PHO5 promoter: from single locus to systems biology of a paradigm for gene regulation through chromatin
    Philipp Korber
    Adolf Butenandt Institute, Molecular Biology, University of Munich, Munich 80336, Germany
    Nucleic Acids Res 42:10888-902. 2014
  4. doi request reprint Active nucleosome positioning beyond intrinsic biophysics is revealed by in vitro reconstitution
    Philipp Korber
    Adolf Butenandt Institut, University of Munich, Schillerstrasse 44, 80336 Munich, Germany
    Biochem Soc Trans 40:377-82. 2012
  5. pmc Evidence for histone eviction in trans upon induction of the yeast PHO5 promoter
    Philipp Korber
    Adolf Butenandt Institut, Universitat Munchen, Schillerstrasse 44, 80336 Munich, Germany
    Mol Cell Biol 24:10965-74. 2004
  6. ncbi request reprint The histone chaperone Asf1 increases the rate of histone eviction at the yeast PHO5 and PHO8 promoters
    Philipp Korber
    Adolf Butenandt Institut, Universitat Munchen, Schillerstrasse 44, 80336 Munich, Germany
    J Biol Chem 281:5539-45. 2006
  7. pmc In vitro reconstitution of PHO5 promoter chromatin remodeling points to a role for activator-nucleosome competition in vivo
    Franziska Ertel
    Adolf Butenandt Institut, University of Munich, Schillerstr 44, 80336 Munich, Germany
    Mol Cell Biol 30:4060-76. 2010
  8. pmc The RSC chromatin remodeling complex has a crucial role in the complete remodeler set for yeast PHO5 promoter opening
    Sanja Musladin
    Laboratory of Biochemistry, Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb 10000, Croatia and Molecular Biology, Adolf Butenandt Institut, University of Munich, Munich 80336, Germany
    Nucleic Acids Res 42:4270-82. 2014
  9. doi request reprint Genome-wide in vitro reconstitution of yeast chromatin with in vivo-like nucleosome positioning
    Nils Krietenstein
    Adolf Butenandt Institut, Molecular Biology Unit, University of Munich, Munich, Germany
    Methods Enzymol 513:205-32. 2012
  10. pmc Differential cofactor requirements for histone eviction from two nucleosomes at the yeast PHO84 promoter are determined by intrinsic nucleosome stability
    Christian J Wippo
    Adolf Butenandt Institut, Universitat Munchen, Munich, Germany
    Mol Cell Biol 29:2960-81. 2009

Collaborators

Detail Information

Publications22

  1. pmc The RSC chromatin remodelling enzyme has a unique role in directing the accurate positioning of nucleosomes
    Christian J Wippo
    Adolf Butenandt Institut, University of Munich, Munich, Germany
    EMBO J 30:1277-88. 2011
    ..Strikingly, RSC could not be replaced by either the closely related SWI/SNF or the Isw2 remodelling enzyme. Thus, we pinpoint that nucleosome positioning specifically depends on the unique properties of the RSC complex...
  2. doi request reprint In vitro reconstitution of in vivo-like nucleosome positioning on yeast DNA
    Christian J Wippo
    Molecular Biology Unit, Adolf Butenandt Institut, University of Munich, Munich, Germany
    Methods Mol Biol 833:271-87. 2012
    ..It may also generate more physiological templates for in vitro studies of, e.g., nucleosome remodeling or transcription through chromatin...
  3. pmc The yeast PHO5 promoter: from single locus to systems biology of a paradigm for gene regulation through chromatin
    Philipp Korber
    Adolf Butenandt Institute, Molecular Biology, University of Munich, Munich 80336, Germany
    Nucleic Acids Res 42:10888-902. 2014
    ....
  4. doi request reprint Active nucleosome positioning beyond intrinsic biophysics is revealed by in vitro reconstitution
    Philipp Korber
    Adolf Butenandt Institut, University of Munich, Schillerstrasse 44, 80336 Munich, Germany
    Biochem Soc Trans 40:377-82. 2012
    ..Collectively, nucleosome positioning in yeast is actively determined by factors beyond intrinsic biophysics, and in steady-state rather than at equilibrium...
  5. pmc Evidence for histone eviction in trans upon induction of the yeast PHO5 promoter
    Philipp Korber
    Adolf Butenandt Institut, Universitat Munchen, Schillerstrasse 44, 80336 Munich, Germany
    Mol Cell Biol 24:10965-74. 2004
    ..This makes a sliding mechanism for the chromatin transition at the PHO5 promoter highly unlikely and points to histone eviction in trans...
  6. ncbi request reprint The histone chaperone Asf1 increases the rate of histone eviction at the yeast PHO5 and PHO8 promoters
    Philipp Korber
    Adolf Butenandt Institut, Universitat Munchen, Schillerstrasse 44, 80336 Munich, Germany
    J Biol Chem 281:5539-45. 2006
    ..e. they both contribute toward the same outcome without being mutually strictly dependent...
  7. pmc In vitro reconstitution of PHO5 promoter chromatin remodeling points to a role for activator-nucleosome competition in vivo
    Franziska Ertel
    Adolf Butenandt Institut, University of Munich, Schillerstr 44, 80336 Munich, Germany
    Mol Cell Biol 30:4060-76. 2010
    ..These data establish an auxiliary role for DNA binding competition between Pho4 and histones in PHO5 promoter chromatin remodeling in vivo...
  8. pmc The RSC chromatin remodeling complex has a crucial role in the complete remodeler set for yeast PHO5 promoter opening
    Sanja Musladin
    Laboratory of Biochemistry, Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb 10000, Croatia and Molecular Biology, Adolf Butenandt Institut, University of Munich, Munich 80336, Germany
    Nucleic Acids Res 42:4270-82. 2014
    ..Therefore, promoter-specific chromatin rather than transactivators determine remodeler requirements. ..
  9. doi request reprint Genome-wide in vitro reconstitution of yeast chromatin with in vivo-like nucleosome positioning
    Nils Krietenstein
    Adolf Butenandt Institut, Molecular Biology Unit, University of Munich, Munich, Germany
    Methods Enzymol 513:205-32. 2012
    ..This system provides a starting point and positive control for the biochemical dissection of nucleosome positioning mechanisms...
  10. pmc Differential cofactor requirements for histone eviction from two nucleosomes at the yeast PHO84 promoter are determined by intrinsic nucleosome stability
    Christian J Wippo
    Adolf Butenandt Institut, Universitat Munchen, Munich, Germany
    Mol Cell Biol 29:2960-81. 2009
    ..Therefore, chromatin cofactor requirements were determined by intrinsic nucleosome stabilities rather than correlated to promoter strength...
  11. doi request reprint Schizosaccharomyces pombe genome-wide nucleosome mapping reveals positioning mechanisms distinct from those of Saccharomyces cerevisiae
    Alexandra B Lantermann
    Adolf Butenandt Institut, University of Munich, Munich, Germany
    Nat Struct Mol Biol 17:251-7. 2010
    ..cerevisiae. Nucleosome positioning mechanisms are evidently not universal but evolutionarily plastic...
  12. ncbi request reprint Histones are incorporated in trans during reassembly of the yeast PHO5 promoter
    Ulrike J Schermer
    Adolf Butenandt Institut, Universitat Munchen, Germany
    Mol Cell 19:279-85. 2005
    ..Promoter closure occurs very rapidly, and the histone chaperones Asf1 and Hir1 as well as the SWI/SNF nucleosome remodeling complex appear to be important for rapid reassembly of nucleosomes at the PHO5 promoter...
  13. pmc CHD1 remodelers regulate nucleosome spacing in vitro and align nucleosomal arrays over gene coding regions in S. pombe
    Julia Pointner
    Adolf Butenandt Institut, University of Munich, Munich, Germany
    EMBO J 31:4388-403. 2012
    ..Impaired arrays in the absence of either or both remodelers may lead to increased cryptic antisense transcription, but overall gene expression levels are only mildly affected...
  14. pmc Nucleosome stability at the yeast PHO5 and PHO8 promoters correlates with differential cofactor requirements for chromatin opening
    Christina Bech Hertel
    Adolf Butenandt Institut, Universitat Munchen, Germany
    Mol Cell Biol 25:10755-67. 2005
    ..Thus we provide evidence for the correlation of inherently more stable chromatin with stricter cofactor requirements...
  15. ncbi request reprint SWRred not shaken; mixing the histones
    Philipp Korber
    Adolf Butenandt Institut, Molekularbiologie, Universitat Munchen, Schillerstr 44, 80336 Munchen, Germany
    Cell 117:5-7. 2004
    ..In connection with the isolation of histone variant specific chaperone complexes, we are beginning to see the mechanisms that mix the histone octamer with intriguing implications for epigenetic inheritance...
  16. doi request reprint Genome-wide mapping of nucleosome positions in Schizosaccharomyces pombe
    Alexandra Lantermann
    Adolf Butenandt Institut, University of Munich, Schillerstr 44, 80336 Munich, Germany
    Methods 48:218-25. 2009
    ..As an independent method to validate genome-wide data for individual loci, we also include a protocol for the determination of locus specific nucleosome positioning by indirect end labeling...
  17. ncbi request reprint Exploring Nucleosome Unwrapping Using DNA Origami
    Jonas J Funke
    Biomedical Center, Molecular Biology, LMU Munich, 82152 Martinsried near Munich, Germany
    Nano Lett 16:7891-7898. 2016
    ..Our spectrometer provides a modular platform with a direct read out to study conformational equilibria for targets from small biomolecules up to large macromolecular assemblies...
  18. ncbi request reprint In vitro assembly of the characteristic chromatin organization at the yeast PHO5 promoter by a replication-independent extract system
    Philipp Korber
    Adolf Butenandt Institut, University of Munich, Schillerstrasse 44, 80336 Munich, Germany
    J Biol Chem 279:35113-20. 2004
    ..This shows that the chromatin structure at the PHO5 promoter represents a self-organizing system in cell-free yeast extracts and provides a promising substrate for in vitro studies with a direct in vivo correlate...
  19. doi request reprint Nucleosome positioning in yeasts: methods, maps, and mechanisms
    Corinna Lieleg
    Adolf Butenandt Institut, University of Munich, Schillerstr 44, 80336, Munich, Germany
    Chromosoma 124:131-51. 2015
    ..While this mechanistic framework emerged clearly during recent years, the involved factors and their mechanisms are still poorly understood and require future efforts combining in vivo and in vitro approaches. ..
  20. pmc Nucleosome spacing generated by ISWI and CHD1 remodelers is constant regardless of nucleosome density
    Corinna Lieleg
    Adolf Butenandt Institute, Molecular Biology, University of Munich, Munich, Germany
    Mol Cell Biol 35:1588-605. 2015
    ..Additional factors are necessary to set physiological spacing in absolute terms...
  21. doi request reprint Nucleosome dynamics and epigenetic stability
    Philipp Korber
    Adolf Butenandt Institute, Ludwig Maximilians University, 80336 Munich, Germany
    Essays Biochem 48:63-74. 2010
    ..All of them may contribute to the assembly of heritable epigenetic structures...
  22. ncbi request reprint Redundancy of chromatin remodeling pathways for the induction of the yeast PHO5 promoter in vivo
    Slobodan Barbaric
    Laboratory of Biochemistry, University of Zagreb, Croatia
    J Biol Chem 282:27610-21. 2007
    ..This demonstrates the complete independence of promoter chromatin opening from downstream PHO5 transcription processes. Finally, the histone variant Htz1 has no prominent role for the kinetics of PHO5 promoter chromatin remodeling...