Adam Grundhoff

Summary

Affiliation: University of Hamburg
Country: Germany

Publications

  1. doi request reprint Merkel cell polyomavirus, a highly prevalent virus with tumorigenic potential
    Adam Grundhoff
    Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Department Virus Genomics, Martinistrasse 52, 20252 Hamburg, Germany Electronic address
    Curr Opin Virol 14:129-37. 2015
  2. doi request reprint Computational prediction of viral miRNAs
    Adam Grundhoff
    Heinrich Pette Institute for Experimental Virology and Immunology, Hamburg, Germany
    Methods Mol Biol 721:143-52. 2011
  3. pmc Virus-encoded microRNAs
    Adam Grundhoff
    Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Martinistr, Hamburg, Germany
    Virology 411:325-43. 2011
  4. pmc A combined computational and microarray-based approach identifies novel microRNAs encoded by human gamma-herpesviruses
    Adam Grundhoff
    Heinrich Pette Institut fur experimentelle Virologie und Immunologie an der Universitat Hamburg, Germany
    RNA 12:733-50. 2006
  5. doi request reprint MicroRNA profiling during human keratinocyte differentiation using a quantitative real-time PCR method
    Janosch Hildebrand
    R and D, Skin Research Center, Beiersdorf AG, Hamburg, Germany
    Methods Mol Biol 961:193-200. 2013
  6. doi request reprint Replication of Merkel cell polyomavirus induces reorganization of promyelocytic leukemia nuclear bodies
    Friederike Neumann
    1 Institute for Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg Eppendorf, Hamburg, Germany
    J Gen Virol 97:2926-2938. 2016
  7. pmc A Comprehensive Analysis of Replicating Merkel Cell Polyomavirus Genomes Delineates the Viral Transcription Program and Suggests a Role for mcv-miR-M1 in Episomal Persistence
    Juliane Marie Theiss
    Research Group Virus Genomics, Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany Institute for Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg Eppendorf, Hamburg, Germany
    PLoS Pathog 11:e1004974. 2015
  8. pmc High-affinity Rb binding, p53 inhibition, subcellular localization, and transformation by wild-type or tumor-derived shortened Merkel cell polyomavirus large T antigens
    Sophie Borchert
    Institute for Medical Microbiology and Virology, University Medical Center Eppendorf, Hamburg, Germany
    J Virol 88:3144-60. 2014
  9. pmc A global analysis of evolutionary conservation among known and predicted gammaherpesvirus microRNAs
    Nicole Walz
    Heinrich Pette Institute for Experimental Virology and Immunology, Martinistrasse 52, D 20251 Hamburg, Germany
    J Virol 84:716-28. 2010
  10. pmc Indication of Horizontal DNA Gene Transfer by Extracellular Vesicles
    Stefanie Fischer
    Research Department Cell and Gene Therapy, Clinic for Stem Cell Transplantation, University Medical Center Hamburg Eppendorf, Hamburg, Germany
    PLoS ONE 11:e0163665. 2016

Collaborators

Detail Information

Publications35

  1. doi request reprint Merkel cell polyomavirus, a highly prevalent virus with tumorigenic potential
    Adam Grundhoff
    Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Department Virus Genomics, Martinistrasse 52, 20252 Hamburg, Germany Electronic address
    Curr Opin Virol 14:129-37. 2015
    ..This review will carve out the specifics of MCPyV biology and discuss unresolved issues to help the reader gain a better understanding of what may differentiate MCPyV from other polyomaviruses. ..
  2. doi request reprint Computational prediction of viral miRNAs
    Adam Grundhoff
    Heinrich Pette Institute for Experimental Virology and Immunology, Hamburg, Germany
    Methods Mol Biol 721:143-52. 2011
    ..Here, we provide a detailed protocol for the prediction of putative miRNA genes using VMir, an ab initio prediction program which we have recently designed specifically to identify pre-miRNAs in viral genomes...
  3. pmc Virus-encoded microRNAs
    Adam Grundhoff
    Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Martinistr, Hamburg, Germany
    Virology 411:325-43. 2011
    ..Key steps forward for the field are discussed, including the need for additional functional studies that utilize surgical viral miRNA mutants combined with relevant models of infection...
  4. pmc A combined computational and microarray-based approach identifies novel microRNAs encoded by human gamma-herpesviruses
    Adam Grundhoff
    Heinrich Pette Institut fur experimentelle Virologie und Immunologie an der Universitat Hamburg, Germany
    RNA 12:733-50. 2006
    ..The advantages and limitations of the approach are discussed...
  5. doi request reprint MicroRNA profiling during human keratinocyte differentiation using a quantitative real-time PCR method
    Janosch Hildebrand
    R and D, Skin Research Center, Beiersdorf AG, Hamburg, Germany
    Methods Mol Biol 961:193-200. 2013
    ..Here, we describe a general real-time PCR method for miRNA profiling using isolated epidermal stem cells, transiently amplifying cells and terminally differentiated keratinocytes of human skin...
  6. doi request reprint Replication of Merkel cell polyomavirus induces reorganization of promyelocytic leukemia nuclear bodies
    Friederike Neumann
    1 Institute for Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg Eppendorf, Hamburg, Germany
    J Gen Virol 97:2926-2938. 2016
    ..While PML and Daxx had no effect on MCPyV DNA replication, MCPyV replication was increased in cells depleted for Sp100, strongly suggesting that Sp100 is a negative regulator of MCPyV DNA replication...
  7. pmc A Comprehensive Analysis of Replicating Merkel Cell Polyomavirus Genomes Delineates the Viral Transcription Program and Suggests a Role for mcv-miR-M1 in Episomal Persistence
    Juliane Marie Theiss
    Research Group Virus Genomics, Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany Institute for Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg Eppendorf, Hamburg, Germany
    PLoS Pathog 11:e1004974. 2015
    ..Our data suggest that MCPyV may have evolved strategies to enter a non- or low level vegetative stage of infection which could aid the virus in establishing and maintaining a lifelong persistence. ..
  8. pmc High-affinity Rb binding, p53 inhibition, subcellular localization, and transformation by wild-type or tumor-derived shortened Merkel cell polyomavirus large T antigens
    Sophie Borchert
    Institute for Medical Microbiology and Virology, University Medical Center Eppendorf, Hamburg, Germany
    J Virol 88:3144-60. 2014
    ....
  9. pmc A global analysis of evolutionary conservation among known and predicted gammaherpesvirus microRNAs
    Nicole Walz
    Heinrich Pette Institute for Experimental Virology and Immunology, Martinistrasse 52, D 20251 Hamburg, Germany
    J Virol 84:716-28. 2010
    ..Although several other gammaherpesviruses are predicted to encode large numbers of clustered miRNAs at conserved genomic loci, no further examples of evolutionarily conserved miRNA sequences were found...
  10. pmc Indication of Horizontal DNA Gene Transfer by Extracellular Vesicles
    Stefanie Fischer
    Research Department Cell and Gene Therapy, Clinic for Stem Cell Transplantation, University Medical Center Hamburg Eppendorf, Hamburg, Germany
    PLoS ONE 11:e0163665. 2016
    ..This mechanism might be of relevance in cancer but also during cell evolution and development...
  11. doi request reprint A transplant "immunome" screening platform defines a targetable epitope fingerprint of multiple myeloma
    Aneta Schieferdecker
    Department of Oncology and Hematology, Bone Marrow Transplantation with Section Pneumology, Hubertus Wald Tumorzentrum University Cancer Center Hamburg, University Medical Center Hamburg Eppendorf, Hamburg, Germany
    Blood 127:3202-14. 2016
    ..Our data reveal a disease-specific epitope signature of MM that is predictive for response to treatment. Mining of transplant immunomes for strong myeloma surface binders may open up avenues for myeloma immunotherapy. ..
  12. doi request reprint In Vitro Replication Assay for Merkel Cell Polyomavirus (MCPyV)
    Friederike Neumann
    Institute for Medical Microbiology, Virology and Hygiene University Medical Center Hamburg Eppendorf, Hamburg, Germany
    Curr Protoc Microbiol 38:14F.2.1-19. 2015
    ..However, serial transmission of infectious virus is still restricted in these cells...
  13. pmc Evaluation of Unbiased Next-Generation Sequencing of RNA (RNA-seq) as a Diagnostic Method in Influenza Virus-Positive Respiratory Samples
    Nicole Fischer
    Institute of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg Eppendorf UKE, Hamburg, Germany German Center for Infection research DZIF, partner site Hamburg Borstel Lübeck, Germany
    J Clin Microbiol 53:2238-50. 2015
    ..Thus, analysis of respiratory samples from known or suspected influenza patients by UMERS provides valuable information that is relevant for clinical investigation. ..
  14. pmc A microRNA encoded by Kaposi sarcoma-associated herpesvirus promotes B-cell expansion in vivo
    Christine Dahlke
    Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany
    PLoS ONE 7:e49435. 2012
    ....
  15. pmc A comparative metagenome survey of the fecal microbiota of a breast- and a plant-fed Asian elephant reveals an unexpectedly high diversity of glycoside hydrolase family enzymes
    Nele Ilmberger
    Universitat Hamburg, Biozentrum Klein Flottbek, Abteilung für Mikrobiologie and Biotechnologie, Hamburg, Germany
    PLoS ONE 9:e106707. 2014
    ..Altogether, this study delivers insight into the biomass conversion by one of the largest plant-fed and land-living animals. ..
  16. pmc Influence of ND10 components on epigenetic determinants of early KSHV latency establishment
    Thomas Gunther
    Research Group Virus Genomics, Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany
    PLoS Pathog 10:e1004274. 2014
    ....
  17. pmc The epigenetic landscape of latent Kaposi sarcoma-associated herpesvirus genomes
    Thomas Gunther
    Heinrich Pette Institute for Experimental Virology and Immunology, Hamburg, Germany
    PLoS Pathog 6:e1000935. 2010
    ..Our findings suggest that the modification patterns identified here induce a poised state of repression during viral latency, which can be rapidly reversed once the lytic cycle is induced...
  18. doi request reprint First report of Escherichia coli co-producing NDM-1 and OXA-232
    Anna Both
    Institute of Medical Microbiology, Virology and Hygiene, University Medical Centre Hamburg Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany
    Diagn Microbiol Infect Dis 86:437-438. 2016
    ..Recently Gram-negative bacteria co-producing multiple carbapenemases have emerged in different parts of the world. We report the first isolation of an Escherichia coli strain co-producing the carbapenemases NDM-1 and OXA-232...
  19. doi request reprint Investigation of Viral and Host Chromatin by ChIP-PCR or ChIP-Seq Analysis
    Thomas Gunther
    Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany
    Curr Protoc Microbiol 40:1E.10.1-21. 2016
    ..Here, we provide protocols that are suitable for performing ChIP-PCR and ChIP-Seq studies on chromatin of large and small viral genomes...
  20. pmc Replication, gene expression and particle production by a consensus Merkel Cell Polyomavirus (MCPyV) genome
    Friederike Neumann
    Institute for Microbiology and Virology, University Medical Center Eppendorf, Hamburg, Germany
    PLoS ONE 6:e29112. 2011
    ..However, serial transmission of infectious virus was not observed. This in vitro culturing system allows the study of viral replication and will facilitate the molecular dissection of important aspects of the MCPyV lifecycle...
  21. doi request reprint Next-generation sequencing of peripheral B-lineage cells pinpoints the circulating clonotypic cell pool in multiple myeloma
    Benjamin Thiele
    University Medical Center Hamburg Eppendorf, Hubertus Wald Tumorzentrum, University Cancer Center Hamburg, Department of Oncology, Hematology, Bone Marrow Transplantation with Section Pneumology, Hamburg, Germany
    Blood 123:3618-21. 2014
    ..Our findings consistently show an absence of pre-switch clonotypic B cells, while M-PCs circulate in the peripheral blood and may contribute to spreading of the disease. ..
  22. pmc Highly significant antiviral activity of HIV-1 LTR-specific tre-recombinase in humanized mice
    Ilona Hauber
    Heinrich Pette Institute Leibniz Institute for Experimental Virology, Hamburg, Germany
    PLoS Pathog 9:e1003587. 2013
    ..Taken together, our data support the use of Tre-recombinase in novel therapy strategies aiming to provide a cure for HIV...
  23. doi request reprint Runx1 is essential at two stages of early murine B-cell development
    Birte Niebuhr
    Molecular Pathology, Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Martinistrasse 52, Hamburg, Germany
    Blood 122:413-23. 2013
    ....
  24. doi request reprint A comprehensive analysis of microRNA expression during human keratinocyte differentiation in vitro and in vivo
    Janosch Hildebrand
    Beiersdorf AG, R and D, Skin Research Center, Hamburg, Germany
    J Invest Dermatol 131:20-9. 2011
    ..Our results thus provide the basis for further analysis of miRNA functions during epidermal differentiation...
  25. pmc Functional Dissection of an Alternatively Spliced Herpesvirus Gene by Splice Site Mutagenesis
    Tim Schommartz
    Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany
    J Virol 90:4626-36. 2016
    ..This study demonstrates the usefulness of splice site mutagenesis for the functional analysis of alternatively spliced herpesvirus genes...
  26. pmc Immunosuppressive Yersinia Effector YopM Binds DEAD Box Helicase DDX3 to Control Ribosomal S6 Kinase in the Nucleus of Host Cells
    Laura Berneking
    Institute of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg Eppendorf UKE, Hamburg, Germany
    PLoS Pathog 12:e1005660. 2016
    ..We propose that the DDX3/CRM1 mediated nucleocytoplasmic shuttling of YopM determines the extent of phosphorylation of RSK in the nucleus to control transcription of immunosuppressive cytokines. ..
  27. pmc Epidermal growth factor receptor mutation mediates cross-resistance to panitumumab and cetuximab in gastrointestinal cancer
    Friederike Braig
    Department of Oncology and Hematology, BMT With Section Pneumology, Hubertus Wald Tumorzentrum UCCH, University Medical Center Hamburg Eppendorf, Hamburg, Germany
    Oncotarget 6:12035-47. 2015
    ....
  28. doi request reprint Merkel cell polyomavirus detection in Merkel cell cancer tumors in Northern Germany using PCR and protein expression
    Miriam Leitz
    Institute for Microbiology and Virology, University Medical Center Eppendorf, Hamburg, Germany
    J Med Virol 86:1813-9. 2014
    ..These data are in accord with published studies describing heterogeneity in MCPyV viral loads and suggest that detection of MCPyV in Merkel cell carcinoma by PCR should be undertaken using multiple primer pairs...
  29. pmc Metagenome survey of a multispecies and alga-associated biofilm revealed key elements of bacterial-algal interactions in photobioreactors
    Ines Krohn-Molt
    University of Hamburg, Biocenter Klein Flottbek, Microbiology and Biotechnology, Hamburg, Germany
    Appl Environ Microbiol 79:6196-206. 2013
    ..This is the first metagenome study of microalga- and PBR-associated biofilm bacteria, and it gives new clues for improved biofuel production in PBRs. ..
  30. pmc Excision of HIV-1 proviral DNA by recombinant cell permeable tre-recombinase
    Lakshmikanth Mariyanna
    Heinrich Pette Institute Leibniz Institute for Experimental Virology, Hamburg, Germany
    PLoS ONE 7:e31576. 2012
    ..The presented data confirm Tre-recombinase activity on integrated HIV-1 and provide the basis for the non-genetic transient application of engineered recombinases, which may be a valuable component of future HIV eradication strategies...
  31. pmc Inefficient establishment of KSHV latency suggests an additional role for continued lytic replication in Kaposi sarcoma pathogenesis
    Adam Grundhoff
    Howard Hughes Medical Institute and Department of Microbiology, University of California, San Francisco, San Francisco, California 94143 0414, USA
    J Clin Invest 113:124-36. 2004
    ..They also suggest an additional role for lytic replication in sustaining KS tumorigenesis: namely, the recruitment of new cells to latency to replace those that have segregated the viral episome...
  32. pmc RNAs in the virion of Kaposi's sarcoma-associated herpesvirus
    Jill Bechtel
    Howard Hughes Medical Institute and G W Hooper Foundation, University of California, San Francisco, CA 94143, USA
    J Virol 79:10138-46. 2005
    ..However, the intracellular levels of one virion mRNA, encoding the viral protease, were much lower than those of transcripts not packaged in the virus particle, strongly suggesting that it may be incorporated by a specific mechanism...
  33. pmc Exploring the DNA binding interactions of the Kaposi's sarcoma-associated herpesvirus lytic switch protein by selective amplification of bound sequences in vitro
    Joseph Ziegelbauer
    G W Hooper Foundation, University of California, San Francisco, Box 0552, 513 Parnassus Ave, San Francisco, California 94143 0552, USA
    J Virol 80:2958-67. 2006
    ..This raises the possibility that some late KSHV genes may also be subject to direct RTA regulation, though indirect models are not excluded...
  34. ncbi request reprint Identification of viral microRNAs
    Christopher S Sullivan
    Department of Molecular Genetics and Microbiology, University of Texas at Austin, Austin, Texas, USA
    Methods Enzymol 427:3-23. 2007
    ..Here, we provide a detailed protocol on how to use computational prediction methods in conjunction with high-throughput microarray analysis to detect miRNAs in viral genomes...
  35. pmc The latency-associated nuclear antigen of Kaposi's sarcoma-associated herpesvirus permits replication of terminal repeat-containing plasmids
    Adam Grundhoff
    Departments of Microbiology and Medicine, Howard Hughes Medical Institute, University of California Medical Center, San Francisco, CA 94143 0414, USA
    J Virol 77:2779-83. 2003
    ....