Wilfried Schwab

Summary

Country: Germany

Publications

  1. doi request reprint Biosynthesis of plant-derived flavor compounds
    Wilfried Schwab
    Biomolecular Food Technology, Technical University Munich, 85354 Freising, Lise Meitner Strasse 34, Germany
    Plant J 54:712-32. 2008
  2. pmc Cloning and characterization of a 9-lipoxygenase gene induced by pathogen attack from Nicotiana benthamiana for biotechnological application
    Fong Chin Huang
    Technische Universitat Munchen, Biotechnology of Natural Products, Liesel Beckmann Str, 1, D 85354 Freising, Germany
    BMC Biotechnol 11:30. 2011
  3. pmc Redirection of flavonoid biosynthesis through the down-regulation of an anthocyanidin glucosyltransferase in ripening strawberry fruit
    Markus Griesser
    Biomolecular Food Technology, Technical University Munich, 85354 Freising, Germany
    Plant Physiol 146:1528-39. 2008
  4. ncbi request reprint Functional characterization of enone oxidoreductases from strawberry and tomato fruit
    Dorothée Klein
    Biomolecular Food Technology, TU Munchen, Lise Meitner Strasse 34, 85354 Freising, Germany
    J Agric Food Chem 55:6705-11. 2007
  5. ncbi request reprint Up- and down-regulation of Fragaria x ananassa O-methyltransferase: impacts on furanone and phenylpropanoid metabolism
    Stefan Lunkenbein
    Technical University Muenchen, Biomolecular Food Technology, Lise Meitner Str 34, D 85354 Freising, Germany
    J Exp Bot 57:2445-53. 2006
  6. pmc Cinnamate metabolism in ripening fruit. Characterization of a UDP-glucose:cinnamate glucosyltransferase from strawberry
    Stefan Lunkenbein
    Biomolecular Food Technology, Technical University Munich, Freising, Germany
    Plant Physiol 140:1047-58. 2006
  7. ncbi request reprint FaGT2: a multifunctional enzyme from strawberry (Fragaria x ananassa) fruits involved in the metabolism of natural and xenobiotic compounds
    Christian Landmann
    Biomolecular Food Technology, Technical University Munich, Lise Meitner Str 34, 85354 Freising, Germany
    Planta 226:417-28. 2007
  8. doi request reprint Substrate promiscuity of RdCCD1, a carotenoid cleavage oxygenase from Rosa damascena
    Fong Chin Huang
    Technische Universitat Munchen, Hochfeldweg 1, D 85354 Freising, Germany
    Phytochemistry 70:457-64. 2009
  9. pmc Metabolic interaction between anthocyanin and lignin biosynthesis is associated with peroxidase FaPRX27 in strawberry fruit
    Ludwig Ring
    Biotechnology of Natural Products, Technische Universitat Munchen, 85354 Freising, Germany
    Plant Physiol 163:43-60. 2013
  10. ncbi request reprint Molecular characterization of a stable antisense chalcone synthase phenotype in strawberry (Fragaria x ananassa)
    Stefan Lunkenbein
    Biomolecular Food Technology, Technical University Munchen, Lise Meitner Strasse 34, 85354 Freising, Germany
    J Agric Food Chem 54:2145-53. 2006

Collaborators

Detail Information

Publications40

  1. doi request reprint Biosynthesis of plant-derived flavor compounds
    Wilfried Schwab
    Biomolecular Food Technology, Technical University Munich, 85354 Freising, Lise Meitner Strasse 34, Germany
    Plant J 54:712-32. 2008
    ..Major progress has resulted from the use of molecular and biochemical techniques, and a large number of genes encoding enzymes of volatile biosynthesis have recently been reported...
  2. pmc Cloning and characterization of a 9-lipoxygenase gene induced by pathogen attack from Nicotiana benthamiana for biotechnological application
    Fong Chin Huang
    Technische Universitat Munchen, Biotechnology of Natural Products, Liesel Beckmann Str, 1, D 85354 Freising, Germany
    BMC Biotechnol 11:30. 2011
    ..In our previous study, we found that enzyme activity of endogenous 9-LOX in Nicotiana benthamiana was highly induced by agroinfiltration using a tobacco mosaic virus (TMV) based vector system...
  3. pmc Redirection of flavonoid biosynthesis through the down-regulation of an anthocyanidin glucosyltransferase in ripening strawberry fruit
    Markus Griesser
    Biomolecular Food Technology, Technical University Munich, 85354 Freising, Germany
    Plant Physiol 146:1528-39. 2008
    ..These results demonstrate a method to redirect the anthocyanin biosynthesis into flavan-3-ol production to increase the levels of bioactive natural products or modify pigments in plant tissues...
  4. ncbi request reprint Functional characterization of enone oxidoreductases from strawberry and tomato fruit
    Dorothée Klein
    Biomolecular Food Technology, TU Munchen, Lise Meitner Strasse 34, 85354 Freising, Germany
    J Agric Food Chem 55:6705-11. 2007
    ..Solanum lycopersicon EO (SlEO) was cloned from cDNA, and the protein was expressed in Escherichia coli and purified. Biochemical studies confirmed the involvement of SlEO in the biosynthesis of HDMF in tomato fruit...
  5. ncbi request reprint Up- and down-regulation of Fragaria x ananassa O-methyltransferase: impacts on furanone and phenylpropanoid metabolism
    Stefan Lunkenbein
    Technical University Muenchen, Biomolecular Food Technology, Lise Meitner Str 34, D 85354 Freising, Germany
    J Exp Bot 57:2445-53. 2006
    ..Thus, FaOMT is involved in at least two different biochemical pathways in ripe strawberry fruit...
  6. pmc Cinnamate metabolism in ripening fruit. Characterization of a UDP-glucose:cinnamate glucosyltransferase from strawberry
    Stefan Lunkenbein
    Biomolecular Food Technology, Technical University Munich, Freising, Germany
    Plant Physiol 140:1047-58. 2006
    ....
  7. ncbi request reprint FaGT2: a multifunctional enzyme from strawberry (Fragaria x ananassa) fruits involved in the metabolism of natural and xenobiotic compounds
    Christian Landmann
    Biomolecular Food Technology, Technical University Munich, Lise Meitner Str 34, 85354 Freising, Germany
    Planta 226:417-28. 2007
    ..The results suggest that FaGT2 is involved in the detoxification of xenobiotics in accordance to its induction by oxidative stress...
  8. doi request reprint Substrate promiscuity of RdCCD1, a carotenoid cleavage oxygenase from Rosa damascena
    Fong Chin Huang
    Technische Universitat Munchen, Hochfeldweg 1, D 85354 Freising, Germany
    Phytochemistry 70:457-64. 2009
    ..The RdCCD1 transcript was present predominantly in rose flower, where high levels of volatile C(13)-norisoprenoids are produced. Thus, the accumulation of C(13)-norisoprenoids in rose flower is correlated to the expression of RdCCD1...
  9. pmc Metabolic interaction between anthocyanin and lignin biosynthesis is associated with peroxidase FaPRX27 in strawberry fruit
    Ludwig Ring
    Biotechnology of Natural Products, Technische Universitat Munchen, 85354 Freising, Germany
    Plant Physiol 163:43-60. 2013
    ..The list of the 15 candidates provides new genes that are likely to impact polyphenol accumulation in strawberry fruit and could be used to develop molecular markers to select phenolics-rich germplasm. ..
  10. ncbi request reprint Molecular characterization of a stable antisense chalcone synthase phenotype in strawberry (Fragaria x ananassa)
    Stefan Lunkenbein
    Biomolecular Food Technology, Technical University Munchen, Lise Meitner Strasse 34, 85354 Freising, Germany
    J Agric Food Chem 54:2145-53. 2006
    ....
  11. doi request reprint Metabolic engineering in strawberry fruit uncovers a dormant biosynthetic pathway
    Thomas Hoffmann
    Biotechnology of Natural Products, Technische Universitat Munchen, Liesel Beckmann Strasse 1, Freising, Germany
    Metab Eng 13:527-31. 2011
    ..Thus, partial restoration of wild strawberry flavor in cultivated varieties is feasible by diverting the flavonoid pathway to phenylpropene synthesis through metabolic engineering...
  12. pmc Methylation of sulfhydryl groups: a new function for a family of small molecule plant O-methyltransferases
    Heather Coiner
    TU Munchen, FG Biomolekulare Lebensmitteltechnologie, Lise Meitner Str 34, D 85354 Freising, Germany
    Plant J 46:193-205. 2006
    ..Future functional investigations of plant methyltransferases should consider the possibility that the enzymes may direct methylation at sulfhydryl groups...
  13. ncbi request reprint Cloning and functional characterization of three terpene synthases from lavender (Lavandula angustifolia)
    Christian Landmann
    Biomolecular Food Technology, Technical University Munich, Lise Meitner Str 34, 85354 Freising, Germany
    Arch Biochem Biophys 465:417-29. 2007
    ..The identification and description of the first terpene synthases from L. angustifolia forms the basis for the biotechnological modification of essential oil composition in lavender...
  14. ncbi request reprint RNAi-induced silencing of gene expression in strawberry fruit (Fragaria x ananassa) by agroinfiltration: a rapid assay for gene function analysis
    Thomas Hoffmann
    Technical University Munchen, Biomolecular Food Technology, Lise Meitner Str 34, 85354 Freising, Germany
    Plant J 48:818-26. 2006
    ..We anticipate that this technique in combination with metabolite profiling analysis will be useful for studying the function of unknown genes during the development and ripening of strawberry fruit...
  15. pmc Cloning and functional characterization of carotenoid cleavage dioxygenase 4 genes
    Fong Chin Huang
    Biomolecular Food Technology, Technische Universitat Munchen, Hochfeldweg 1, D 85354 Freising, Germany
    J Exp Bot 60:3011-22. 2009
    ....
  16. doi request reprint Absorption of 3(2H)-furanones by human intestinal epithelial Caco-2 cells
    Nicole Christina Stadler
    Biomolecular Food Technology, Technical University Munchen, Hochfeldweg 1, 85354 Freising, Germany
    J Agric Food Chem 57:3949-54. 2009
    ..Because the uptake was only slightly reduced by apical hyperosmolarity, passive diffusion by paracellular transport is proposed...
  17. ncbi request reprint The carotenase AtCCD1 from Arabidopsis thaliana is a dioxygenase
    Holger Schmidt
    Foundation for Biomolecular Food Technology, Technische Universitat Munchen, Lise Meitner Strasse 34, D 84354 Freising, Germany
    J Biol Chem 281:9845-51. 2006
    ..The results unambiguously demonstrate a dioxygenase mechanism for the carotenase AtCCD1 from A. thaliana...
  18. pmc Comparative analysis of benzoxazinoid biosynthesis in monocots and dicots: independent recruitment of stabilization and activation functions
    Regina Dick
    Lehrstuhl fur Genetik, Wissenschaftszentrum Weihenstephan, Technische Universitat Munchen, 85354 Freising, Germany
    Plant Cell 24:915-28. 2012
    ..The data reveal a great evolutionary flexibility in recruitment of these essential functions of secondary plant metabolism...
  19. doi request reprint Substrate promiscuity of a rosmarinic acid synthase from lavender (Lavandula angustifolia L.)
    Christian Landmann
    Biotechnology of Natural Products, Technische Universitaet Muenchen, Liesel Beckmann Str 1, 85354, Freising, Germany
    Planta 234:305-20. 2011
    ..LaAT2 is one of three members of clade VI with unknown function...
  20. doi request reprint Natural 4-hydroxy-2,5-dimethyl-3(2H)-furanone (Furaneol®)
    Wilfried Schwab
    Biotechnology of Natural Products, Technische Universitat Munchen, Liesel Beckmann Str 1, 85354 Freising, Germany
    Molecules 18:6936-51. 2013
    ..During the last years substantial progress has been made in the elucidation of the biological pathway leading to HDMF and DMMF. This review summarizes the latest advances in this field...
  21. doi request reprint A double mutation in the anthocyanin 5-O-glucosyltransferase gene disrupts enzymatic activity in Vitis vinifera L
    László Jánváry
    Biomolecular Food Technology, Technische Universitat Munchen, Hochfeldweg 1, 85354 Freising, Germany
    J Agric Food Chem 57:3512-8. 2009
    ..vinifera and explain why revertants for this mutant allele have not been observed in breeding programs. The results have a significant effect on the classification and breeding of Vitis varieties and the evaluation of derived products...
  22. doi request reprint Overexpression of hydroperoxide lyase gene in Nicotiana benthamiana using a viral vector system
    Fong Chin Huang
    Technische Universitat Munchen, Biomolecular Food Technology, Freising, Germany
    Plant Biotechnol J 8:783-95. 2010
    ..benthamiana leaves over-expressing ClHPL in combination with GmVLXC-expressing yeast extracts. One gram of ClHPL-expressing N. benthamiana leaves (fresh weight) could produce 17 +/- 0.4 mg hexanal from 50 mg linoleic acid after 30 min...
  23. doi request reprint Benzoxazinoid biosynthesis in dicot plants
    Katrin Schullehner
    Lehrstuhl fur Genetik, Technische Universitat Munchen, Am Hochanger 8, 85350 Freising, Germany
    Phytochemistry 69:2668-77. 2008
    ..C. orientalis has an active indole-3-glycerolphosphate lyase for indole formation that evolved independently from its orthologous function in maize. The properties and evolution of plant indole-3-glycerolphosphate lyases are discussed...
  24. pmc Glucosylation of 4-Hydroxy-2,5-Dimethyl-3(2H)-Furanone, the Key Strawberry Flavor Compound in Strawberry Fruit
    Chuankui Song
    Biotechnology of Natural Products, Technische Universitat Munchen, Liesel Beckmann Str 1, 85354 Freising, Germany
    Plant Physiol 171:139-51. 2016
    ..These results provide the foundation for improvement of strawberry flavor and the biotechnological production of HDMF-glucoside. ..
  25. pmc Multi-substrate flavonol O-glucosyltransferases from strawberry (Fragaria x ananassa) achene and receptacle
    Markus Griesser
    Biomolecular Food Technology, Technical University Munich, Lise Meitner Str 34, 85354 Freising, Germany
    J Exp Bot 59:2611-25. 2008
    ..This report presents the first biochemical characterization of enzymes mainly expressed in strawberry achenes and provides the foundation of flavonoid metabolism in the seeds...
  26. ncbi request reprint Molecular interaction between Methylobacterium extorquens and seedlings: growth promotion, methanol consumption, and localization of the methanol emission site
    Daniel Abanda-Nkpwatt
    Biomolecular Food Technology, Technical University of Munich, Lise Meitner Strasse 34, D 85354 Freising, Germany
    J Exp Bot 57:4025-32. 2006
    ..Methylobacterium extorquens strains can nourish themselves using the methanol released by the stomata and release an agent promoting the growth of the seedlings of some crop plants...
  27. ncbi request reprint Biocatalytic production of dihydrocoumarin from coumarin by Saccharomyces cerevisiae
    Katrin Häser
    Degussa AG, Lise Meitner Strasse 34, 85354 Freising, Germany
    J Agric Food Chem 54:6236-40. 2006
    ..cerevisiae within 147 h. This dihydrocoumarin thus fulfills all of the criteria of a natural raw material and can be used as a natural flavoring in accordance with U.S. and European Union regulations...
  28. doi request reprint Heterotic patterns of sugar and amino acid components in developing maize kernels
    Lilla Römisch-Margl
    Lehrstuhl fur Genetik, Wissenschaftszentrum Weihenstephan, Technische Universitat Munchen, Emil Ramann Str 8, 85354 Freising, Germany
    Theor Appl Genet 120:369-81. 2010
    ..Our findings illustrate the power of metabolomics to characterize heterotic maize lines and suggest that the metabolite composition is a potential marker in the context of heterosis research...
  29. ncbi request reprint Maize Lc transcription factor enhances biosynthesis of anthocyanins, distinct proanthocyanidins and phenylpropanoids in apple (Malus domestica Borkh.)
    Houhua Li
    Institute of Biological Production Systems, Fruit Science Section, Leibniz University of Hannover, Herrenhaeuser Str 2, 30419 Hannover, Germany
    Planta 226:1243-54. 2007
    ..Thus, Lc overexpression in Malus domestica resulted in enhanced biosynthesis of specific flavonoid classes, which play important roles in both phytopathology and human health...
  30. ncbi request reprint Chemical formation of 4-hydroxy-2,5-dimethyl-3[2H]-furanone from D-fructose 1,6-diphosphate
    Tobias Hauck
    Lehrstuhl fur Lebensmittelchemie, Universitat Wurzburg, Am Hubland, D 97074, Wurzburg, Germany
    Carbohydr Res 337:1185-91. 2002
    ....
  31. ncbi request reprint Isolation, cloning and expression of a multifunctional O-methyltransferase capable of forming 2,5-dimethyl-4-methoxy-3(2H)-furanone, one of the key aroma compounds in strawberry fruits
    Martina Wein
    , , Am Hubland, , Germany
    Plant J 31:755-65. 2002
    ..In addition, it is concluded that the Strawberry-OMT plays an important role in the biosynthesis of strawberry volatiles such as vanillin and DMMF...
  32. ncbi request reprint Metabolome diversity: too few genes, too many metabolites?
    Wilfried Schwab
    Lehrstuhl fur Lebensmittelchemie, Universitat Wurzburg, Am Hubland, D 97074, Wurzburg, Germany
    Phytochemistry 62:837-49. 2003
    ..It is concluded that metabolome diversity is caused by low enzyme specificity but availability of suitable substrates due to compartmentation has also taken into account...
  33. pmc Formation of 4-hydroxy-2,5-dimethyl-3[2H]-furanone by Zygosaccharomyces rouxii: identification of an intermediate
    Tobias Hauck
    Lehrstuhl fur Lebensmittelchemie, Universitat Wurzburg, 97074 Wurzburg, Germany
    Appl Environ Microbiol 69:3911-8. 2003
    ..Incubation of periplasmic and cytosolic protein extracts obtained from yeast cells with D-fructose-1,6-bisphosphate led to the formation of HDMF, implying the presence of the required enzymes in both extracts...
  34. ncbi request reprint Tautomerism of 4-hydroxy-2,5-dimethyl-3(2H)-furanone: evidence for its enantioselective biosynthesis
    Thomas Raab
    Chair of Food Chemistry, University of Wurzburg, Am Hubland, Wurzburg, Germany
    Chirality 15:573-8. 2003
    ..In both experiments the formation of enantiomerically enriched HDMF could be demonstrated for the first time, whereas incubation experiments under neutral conditions resulted in the detection of racemic HDMF...
  35. ncbi request reprint Alternative pathway for the formation of 4,5-dihydroxy-2,3-pentanedione, the proposed precursor of 4-hydroxy-5-methyl-3(2H)-furanone as well as autoinducer-2, and its detection as natural constituent of tomato fruit
    Tobias Hauck
    Lehrstuhl fur Lebensmittelchemie, Universitat Wurzburg, Am Hubland, 97074 Wurzburg, Germany
    Biochim Biophys Acta 1623:109-19. 2003
    ..Application of o-phenylenediamine into ripe tomatoes led to the detection of 3-(1,2-dihydroxyethyl)-2-methylquinoxaline by means of HPLC-MS/MS analysis implying the genuine occurrence of 4,5-dihydroxy-2,3-pentanedione in this fruit...
  36. pmc Increased and altered fragrance of tobacco plants after metabolic engineering using three monoterpene synthases from lemon
    Joost Lücker
    Business Unit Bioscience, Plant Research International, P O Box 16, 6700 AA, Wageningen, The Netherlands
    Plant Physiol 134:510-9. 2004
    ..The results show that there is a sufficiently high level of substrate accessible for the introduced enzymes...
  37. ncbi request reprint Flavonoid methylation: a novel 4'-O-methyltransferase from Catharanthus roseus, and evidence that partially methylated flavanones are substrates of four different flavonoid dioxygenases
    Gudrun Schröder
    Institut fur Biologie II, Universitat Freiburg, D 79104 Freiburg, Germany
    Phytochemistry 65:1085-94. 2004
    ..These results characterized a new type of flavonoid O-methyltransferase, and also provided new insights into the catalytic capacities of key dioxygenases in flavonoid biosynthesis...
  38. ncbi request reprint Metabolic engineering of monoterpene biosynthesis: two-step production of (+)-trans-isopiperitenol by tobacco
    Joost Lücker
    Business Unit Bioscience, Plant Research International, PO Box 16, 6700 AA Wageningen, The Netherlands
    Plant J 39:135-45. 2004
    ..Some further modifications of the (+)-trans-isopiperitenol were also detected, resulting in the additional emission of 1,3,8-p-menthatriene, 1,5,8-p-menthatriene, p-cymene and isopiperitenone...
  39. ncbi request reprint Anthocyanidin synthase from Gerbera hybrida catalyzes the conversion of (+)-catechin to cyanidin and a novel procyanidin
    Frank Wellmann
    Institut fur Pharmazeutische Biologie, Philipps Universitat Marburg, Deutschhausstrasse 17 A, D 35037 Marburg, Germany
    FEBS Lett 580:1642-8. 2006
    ..Moreover, the enzymatic dimerization of catechin monomers is reported for the first time suggesting a role for ANS beyond the oxidation of leucocyanidins...
  40. ncbi request reprint Absolute configuration of 3-hydroxy acids formed by Stenotrophomonas maltophilia: application of multidimensional gas chromatography and circular dichroism spectroscopy
    Kerstin Weil
    Universitat Wurzburg, Lehrstuhl fur Lebensmittelchemie, Am Hubland, D 97074 Wurzburg, Germany
    Chirality 14:51-8. 2002
    ..Their CD spectra displayed a positive first Cotton effect around 254 nm and a negative second Cotton effect around 237 nm, which confirmed the (R)-configuration of the bacterial products...