W Buckel

Summary

Affiliation: Philipps University
Country: Germany

Publications

  1. ncbi request reprint Unusual enzymes involved in five pathways of glutamate fermentation
    W Buckel
    Laboratorium für Mikrobiologie, Fachbereich Biologie, Philipps Universitat, Marburg, Germany
    Appl Microbiol Biotechnol 57:263-73. 2001
  2. ncbi request reprint Sodium ion-translocating decarboxylases
    W Buckel
    Laboratorium für Mikrobiologie, Fachbereich Biologie, Philipps Universitat, D 35032, Marburg, Germany
    Biochim Biophys Acta 1505:15-27. 2001
  3. ncbi request reprint Sodium ion-dependent hydrogen production in Acidaminococcus fermentans
    U Härtel
    Laboratorium für Mikrobiologie, Fachbereich Biologie, Philipps Universitat, D 35032 Marburg, Germany
    Arch Microbiol 166:350-6. 1996
  4. ncbi request reprint Fermentation of trans-aconitate via citrate, oxaloacetate, and pyruvate by Acidaminococcus fermentans
    U Härtel
    Laboratorium für Mikrobiologie, Fachbereich Biologie, Philipps Universitat, D 35032 Marburg, Germany
    Arch Microbiol 166:342-9. 1996
  5. ncbi request reprint Unusual dehydrations in anaerobic bacteria: considering ketyls (radical anions) as reactive intermediates in enzymatic reactions
    W Buckel
    Laboratorium für Mikrobiologie, Philipps Universitat, Marburg, Germany
    FEBS Lett 389:20-4. 1996
  6. ncbi request reprint Cloning, sequencing and expression in Escherichia coli of the gene encoding component S of the coenzyme B12-dependent glutamate mutase from Clostridium cochlearium
    O Zelder
    Laboratorium für Mikrobiologie, Fachbereich Biologie, Philips Universität, Marburg, FRG
    FEMS Microbiol Lett 118:15-21. 1994
  7. ncbi request reprint L-serine and L-threonine dehydratase from Clostridium propionicum. Two enzymes with different prosthetic groups
    A E Hofmeister
    Laboratorium für Mikrobiologie des Fachbereichs Biologie der Philipps Universität, Marburg, Germany
    Eur J Biochem 215:341-9. 1993
  8. ncbi request reprint 2-hydroxyglutaryl-CoA dehydratase from Clostridium symbiosum
    M Hans
    Laboratorium für Mikrobiologie, Fachbereich Biologie, Philipps Universitat, Marburg, Germany
    Eur J Biochem 265:404-14. 1999
  9. ncbi request reprint 2-Methylisocitrate lyases from the bacterium Escherichia coli and the filamentous fungus Aspergillus nidulans: characterization and comparison of both enzymes
    M Brock
    Laboratorium für Mikrobiologie, Fachbreich Biologie, Philipps Universitat, Marburg, Germany
    Eur J Biochem 268:3577-86. 2001
  10. ncbi request reprint Identification of the gene encoding the activator of (R)-2-hydroxyglutaryl-CoA dehydratase from Acidaminococcus fermentans by gene expression in Escherichia coli
    K Bendrat
    Laboratorium für Mikrobiologie, Fachbereich Biologie, Philipps Universitat, Marburg, Germany
    FEBS Lett 329:329-31. 1993

Collaborators

Detail Information

Publications16

  1. ncbi request reprint Unusual enzymes involved in five pathways of glutamate fermentation
    W Buckel
    Laboratorium für Mikrobiologie, Fachbereich Biologie, Philipps Universitat, Marburg, Germany
    Appl Microbiol Biotechnol 57:263-73. 2001
    ..The pathway involves the oxidative dehydration of 5-hydroxyvaleryl-CoA to 2,4-pentadienoyl-CoA followed by reduction to 3-pentenoyl-CoA and isomerisation to 2-pentenoyl-CoA...
  2. ncbi request reprint Sodium ion-translocating decarboxylases
    W Buckel
    Laboratorium für Mikrobiologie, Fachbereich Biologie, Philipps Universitat, D 35032, Marburg, Germany
    Biochim Biophys Acta 1505:15-27. 2001
    ..In many sequenced genomes from Bacteria and Archaea homologues of the carboxybiotin decarboxylase from A. fermentans with up to 80% sequence identity have been detected...
  3. ncbi request reprint Sodium ion-dependent hydrogen production in Acidaminococcus fermentans
    U Härtel
    Laboratorium für Mikrobiologie, Fachbereich Biologie, Philipps Universitat, D 35032 Marburg, Germany
    Arch Microbiol 166:350-6. 1996
    ..Hydrogen production commences exactly at those Na+ concentrations at which the electrogenic H+/Na+-antiporter glutaconyl-CoA decarboxylase is converted into a Na+/Na+ exchanger...
  4. ncbi request reprint Fermentation of trans-aconitate via citrate, oxaloacetate, and pyruvate by Acidaminococcus fermentans
    U Härtel
    Laboratorium für Mikrobiologie, Fachbereich Biologie, Philipps Universitat, D 35032 Marburg, Germany
    Arch Microbiol 166:342-9. 1996
    ..Citrate Si-lyase, a Mg2+-dependent oxaloacetate decarboxylase, and pyruvate synthase were present in quantities that corresponded to the growth rate of the organism...
  5. ncbi request reprint Unusual dehydrations in anaerobic bacteria: considering ketyls (radical anions) as reactive intermediates in enzymatic reactions
    W Buckel
    Laboratorium für Mikrobiologie, Philipps Universitat, Marburg, Germany
    FEBS Lett 389:20-4. 1996
    ....
  6. ncbi request reprint Cloning, sequencing and expression in Escherichia coli of the gene encoding component S of the coenzyme B12-dependent glutamate mutase from Clostridium cochlearium
    O Zelder
    Laboratorium für Mikrobiologie, Fachbereich Biologie, Philips Universität, Marburg, FRG
    FEMS Microbiol Lett 118:15-21. 1994
    ..A rapid purification procedure consisting only of ammonium sulfate precipitation and a gel filtration step was developed to obtain large amounts of pure component S in a short time...
  7. ncbi request reprint L-serine and L-threonine dehydratase from Clostridium propionicum. Two enzymes with different prosthetic groups
    A E Hofmeister
    Laboratorium für Mikrobiologie des Fachbereichs Biologie der Philipps Universität, Marburg, Germany
    Eur J Biochem 215:341-9. 1993
    ..Moreover, the N-terminal sequences of the small subunits from these two organisms were found to be 47% identical. In addition, 38% identity with the N-terminus of one of the two L-serine dehydratases of Escherichia coli was detected...
  8. ncbi request reprint 2-hydroxyglutaryl-CoA dehydratase from Clostridium symbiosum
    M Hans
    Laboratorium für Mikrobiologie, Fachbereich Biologie, Philipps Universitat, Marburg, Germany
    Eur J Biochem 265:404-14. 1999
    ....
  9. ncbi request reprint 2-Methylisocitrate lyases from the bacterium Escherichia coli and the filamentous fungus Aspergillus nidulans: characterization and comparison of both enzymes
    M Brock
    Laboratorium für Mikrobiologie, Fachbreich Biologie, Philipps Universitat, Marburg, Germany
    Eur J Biochem 268:3577-86. 2001
    ..coli. PrpB exhibits 34% sequence identity with carboxyphosphoenolpyruvate phosphonomutase from Streptomyces hygroscopicus, in which the essential cysteine residue is conserved...
  10. ncbi request reprint Identification of the gene encoding the activator of (R)-2-hydroxyglutaryl-CoA dehydratase from Acidaminococcus fermentans by gene expression in Escherichia coli
    K Bendrat
    Laboratorium für Mikrobiologie, Fachbereich Biologie, Philipps Universitat, Marburg, Germany
    FEBS Lett 329:329-31. 1993
    ..Dehydratase activity was stimulated at least tenfold by cell-free extracts of E. coli cells transformed with a plasmid carrying hgdC. On the chromosome the hgdC gene is located just before hgdA and hgdB...
  11. ncbi request reprint Characterization of the coenzyme-B12-dependent glutamate mutase from Clostridium cochlearium produced in Escherichia coli
    O Zelder
    Laboratorium für Mikrobiologie, Fachbereich Biologie, Philipps Universitat Marburg, Germany
    Eur J Biochem 226:577-85. 1994
    ..abstract truncated at 400 words)..
  12. ncbi request reprint Methylcitrate synthase from Aspergillus nidulans: implications for propionate as an antifungal agent
    M Brock
    Laboratorium für Mikrobiologie, Fachbereich Biologie, Philipps Universitat, D 35032 Marburg, Germany
    Mol Microbiol 35:961-73. 2000
    ..Propionate (100 mM) not only impaired hyphal growth of A. nidulans but also synthesis of the green polyketide-derived pigment of the conidia, whereas in the mutant pigmentation was abolished with 20 mM propionate...
  13. ncbi request reprint Propionate oxidation in Escherichia coli: evidence for operation of a methylcitrate cycle in bacteria
    S Textor
    Laboratorium für Mikrobiologie, Fachbereich Biologie, Phillips Universitat, D 35032 Marburg, Germany
    Arch Microbiol 168:428-36. 1997
    ..The 13C patterns of aspartate and glutamate are consistent with the further oxidation of pyruvate to acetyl-CoA. Oxaloacetate is predominantly generated via the glyoxylate cycle rather than by carboxylation of phosphoenolpyruvate...
  14. ncbi request reprint Oxygen exchange between acetate and the catalytic glutamate residue in glutaconate CoA-transferase from Acidaminococcus fermentans. Implications for the mechanism of CoA-ester hydrolysis
    T Selmer
    Laboratorium für Mikrobiologie, Fachbereich Biologie, Philipps Universitat, D 35032 Marburg, Germany
    J Biol Chem 274:20772-8. 1999
    ..The small acetate molecule in the substrate binding pocket may activate a water molecule for hydrolysis of the nearby enzyme-CoA thiol ester...
  15. ncbi request reprint Glutamate mutase from Clostridium cochlearium. Purification, cobamide content and stereospecific inhibitors
    U Leutbecher
    Laboratorium für Mikrobiologie, Fachbereich Biologie, Philipps Universitat, Marburg, Federal Republic of Germany
    Eur J Biochem 205:759-65. 1992
    ..However, the presence of an N-terminal pyruvoyl residue was excluded and no evidence for the participation of another electrophilic center in the reaction was obtained...
  16. ncbi request reprint Bacterial L-serine dehydratases: a new family of enzymes containing iron-sulfur clusters
    R Grabowski
    Laboratorium für Mikrobiologie, Fachbereich Biologie, Philipps Universitat, Marburg, Germany
    Trends Biochem Sci 18:297-300. 1993
    ..A mechanism for the dehydration of L-serine which is similar, but not identical, to that of the dehydration of citrate catalysed by aconitase is proposed...