- Generation of an immortalized human CD4+ T cell clone inhibiting tumor growth in miceG Pecher
AG Molecular Gene and Immunotherapy, Humboldt University Berlin, Charite Campus Mitte, Hessische Strasse 3 4, Berlin, 10115, Germany
Biochem Biophys Res Commun 283:738-42. 2001..SITAM cells provide an unlimited source of clonal T cells for analysis of tumor recognition and may be of help in TCR-targeted immunotherapy...
- Mucin gene (MUC1) transfer into human dendritic cells by cationic liposomes and recombinant adenovirusG Pecher
Charite, Humboldt University Berlin, AG Molecular Gene and Immunotherapy, Germany
Anticancer Res 21:2591-6. 2001..Mucin, encoded by the gene MUC1, is a human tumor antigen expressed in breast, pancreatic and ovarian cancers. Therefore, MUC1-transfected DC would be an attractive tool in constructing cancer vaccines...
- Efficient cryopreservation of dendritic cells transfected with cDNA of a tumour antigen for clinical applicationG Pecher
Humboldt University Berlin, Charite Campus Mitte, AG Molecular Gene and Immunotherapy, Hessische Str 3 4, 10115 Berlin, Germany
Biotechnol Appl Biochem 34:161-6. 2001..The stimulating capacity of a mixed lymphocyte culture was also preserved. These findings offer an efficient method for the cryopreservation of DCs for use in clinical trials...
- Long-term tumor growth suppression in mice immunized with naked DNA of the human tumor antigen mucin (MUC1)H Johnen
Humboldt-University Berlin, , AG Molecular Gene- and Immunotherapy, Berlin, Germany
Cancer Immunol Immunother 50:356-60. 2001..These encouraging results, showing long-term protection against tumor growth, indicate the potential usefulness of naked DNA vaccination for clinical immunotherapy...
- Wilms' tumor gene (WT1) expression in lung cancer, colon cancer and glioblastoma cell lines compared to freshly isolated tumor specimensH D Menssen
Medizinische Klinik III, Hämatologie, Onkologie und Transfusionsmedizin, Benjamin Franklin Klinik der Freien Universität, Berlin, Germany
J Cancer Res Clin Oncol 126:226-32. 2000..From this evidence, testing for WT1 expression is not clinically relevant for colon cancer, lung cancer, or glioblastoma patients. WT1 expression in cancer cell lines can probably be attributed to optimized in vitro growth conditions...