- Structural characterisation of neuronal voltage-sensitive K+ channels heterologously expressed in Pichia pastorisDavid N Parcej
Department of Structural Biology, Max Planck Institute for Biophysics, 60439 Frankfurt am Main, Germany
J Mol Biol 333:103-16. 2003..Projection maps of negatively stained crystals show the constituent molecules to be 4-fold symmetric, as expected for the octameric K(+) channel complex...
- Over-expression of a mammalian small conductance calcium-activated K+ channel in Pichia pastoris: effects of trafficking signals and subunit fusionsLuana Licata
Department of Structural Biology, Max Planck Institute for Biophysics, Frankfurt am Main, Germany
Protein Expr Purif 47:171-8. 2006..Fusion of SK2 with the associated protein calmodulin also altered the channel localisation but in a different manner with channels now found mainly in transit between endoplasmic reticulum and Golgi compartments...