Ulrich Arnold

Summary

Affiliation: Martin Luther University
Country: Germany

Publications

  1. ncbi Aspects of the cytotoxic action of ribonucleases
    Ulrich Arnold
    Martin Luther University Halle Wittenberg, Institute of Biochemistry and Biotechnology, Kurt Mothes Str 3, 06120 Halle, Germany
    Curr Pharm Biotechnol 9:161-8. 2008
  2. ncbi Protein prosthesis: a semisynthetic enzyme with a beta-peptide reverse turn
    Ulrich Arnold
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Am Chem Soc 124:8522-3. 2002
  3. ncbi Natural and engineered ribonucleases as potential cancer therapeutics
    Ulrich Arnold
    Department of Biochemistry Biotechnology, Martin Luther University, Kurt Mothes Strasse 3, 06120, Halle, Germany
    Biotechnol Lett 28:1615-22. 2006
  4. ncbi Therapeutic potential of RNases
    Ulrich Arnold
    Curr Pharm Biotechnol 9:134. 2008
  5. doi Significant stabilization of ribonuclease A by additive effects
    Ulrich Arnold
    Institute of Biochemistry and Biotechnology, Martin Luther University Halle Wittenberg, Halle, Germany
    FEBS J 279:2508-19. 2012
  6. doi Incorporation of non-natural modules into proteins: structural features beyond the genetic code
    Ulrich Arnold
    Institute of Biochemistry and Biotechnology, Martin Luther University, Kurt Mothes Str 3, 06120, Halle, Germany
    Biotechnol Lett 31:1129-39. 2009
  7. ncbi Contribution of structural peculiarities of onconase to its high stability and folding kinetics
    Ulrich Arnold
    Department of Biochemistry Biotechnology, Martin Luther University Halle Wittenberg, Kurt Mothes Strasse 3, 06120 Halle, Germany
    Biochemistry 45:3580-7. 2006
  8. doi Crystal structure of RNase A tandem enzymes and their interaction with the cytosolic ribonuclease inhibitor
    Ulrich Arnold
    Department of Biochemistry and Biotechnology, Martin Luther University Halle Wittenberg, Halle, Germany
    FEBS J 278:331-40. 2011
  9. ncbi Probing the unfolding region of ribonuclease A by site-directed mutagenesis
    Jens Köditz
    Department of Biochemistry and Biotechnology, Martin Luther University Halle Wittenberg, Halle, Germany
    Eur J Biochem 271:4147-56. 2004
  10. doi Impact of the C-terminal disulfide bond on the folding and stability of onconase
    Cindy Schulenburg
    Institute of Biochemistry and Biotechnology, Martin Luther University Halle Wittenberg, Kurt Mothes Strasse 3, 06120 Halle, Germany
    Chembiochem 11:978-86. 2010

Collaborators

Detail Information

Publications25

  1. ncbi Aspects of the cytotoxic action of ribonucleases
    Ulrich Arnold
    Martin Luther University Halle Wittenberg, Institute of Biochemistry and Biotechnology, Kurt Mothes Str 3, 06120 Halle, Germany
    Curr Pharm Biotechnol 9:161-8. 2008
    ..In the present review I will summarize the status quo of the knowledge on the mechanisms and their interdependence as well as to develop strategies to overcome possible limitations...
  2. ncbi Protein prosthesis: a semisynthetic enzyme with a beta-peptide reverse turn
    Ulrich Arnold
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Am Chem Soc 124:8522-3. 2002
    ..The semisynthetic enzyme not only retains full catalytic activity but also gains conformational stability. Thus, structural elements can be replaced with foldameric equivalents to endow proteins with more desirable properties...
  3. ncbi Natural and engineered ribonucleases as potential cancer therapeutics
    Ulrich Arnold
    Department of Biochemistry Biotechnology, Martin Luther University, Kurt Mothes Strasse 3, 06120, Halle, Germany
    Biotechnol Lett 28:1615-22. 2006
    ..This paper discusses the various approaches to exploit RNases as cytotoxic agents...
  4. ncbi Therapeutic potential of RNases
    Ulrich Arnold
    Curr Pharm Biotechnol 9:134. 2008
  5. doi Significant stabilization of ribonuclease A by additive effects
    Ulrich Arnold
    Institute of Biochemistry and Biotechnology, Martin Luther University Halle Wittenberg, Halle, Germany
    FEBS J 279:2508-19. 2012
    ..The analysis of the crystal structure of A4C/D83E/V118C-ribonuclease A reveals the formation of a salt bridge between the γ-carboxyl group of Glu83 and the ε-amino group of Lys104...
  6. doi Incorporation of non-natural modules into proteins: structural features beyond the genetic code
    Ulrich Arnold
    Institute of Biochemistry and Biotechnology, Martin Luther University, Kurt Mothes Str 3, 06120, Halle, Germany
    Biotechnol Lett 31:1129-39. 2009
    ....
  7. ncbi Contribution of structural peculiarities of onconase to its high stability and folding kinetics
    Ulrich Arnold
    Department of Biochemistry Biotechnology, Martin Luther University Halle Wittenberg, Kurt Mothes Strasse 3, 06120 Halle, Germany
    Biochemistry 45:3580-7. 2006
    ..Our data on the folding and unfolding kinetics indicate that the strong stabilization of ONC in comparison to RNase A is caused by a dramatic deceleration of the unfolding reaction...
  8. doi Crystal structure of RNase A tandem enzymes and their interaction with the cytosolic ribonuclease inhibitor
    Ulrich Arnold
    Department of Biochemistry and Biotechnology, Martin Luther University Halle Wittenberg, Halle, Germany
    FEBS J 278:331-40. 2011
    ..As other dimeric RNases, which are comparably cytotoxic, either evade RI binding or potentially even bind two RI molecules, inactivation by RI cannot be the crucial limitation to the cytotoxicity of dimeric RNases...
  9. ncbi Probing the unfolding region of ribonuclease A by site-directed mutagenesis
    Jens Köditz
    Department of Biochemistry and Biotechnology, Martin Luther University Halle Wittenberg, Halle, Germany
    Eur J Biochem 271:4147-56. 2004
    ....
  10. doi Impact of the C-terminal disulfide bond on the folding and stability of onconase
    Cindy Schulenburg
    Institute of Biochemistry and Biotechnology, Martin Luther University Halle Wittenberg, Kurt Mothes Strasse 3, 06120 Halle, Germany
    Chembiochem 11:978-86. 2010
    ..The formation of the on-pathway intermediate, which is detectable during the folding of the wild-type protein and promotes the fast and efficient refolding reaction, requires the presence of this covalent bond...
  11. doi The folding pathway of onconase is directed by a conserved intermediate
    Cindy Schulenburg
    Institute of Biochemistry and Biotechnology, Martin Luther University Halle Wittenberg, Kurt Mothes Strasse 3, 06120 Halle, Germany
    Biochemistry 48:8449-57. 2009
    ..Obviously, in spite of considerable differences in their amino acid sequence the initial folding events of both proteins are comparable, guided by a limited number of conserved residues...
  12. doi The effect of additional disulfide bonds on the stability and folding of ribonuclease A
    Pascal Pecher
    Martin Luther University Halle Wittenberg, Institute of Biochemistry and Biotechnology, Germany
    Biophys Chem 141:21-8. 2009
    ..Native-state proteolysis indicated a perturbation of the native state of the destabilized variants that obviously counterbalances the stability gain by the extra disulfide bond...
  13. doi Consequences of proline-to-alanine substitutions for the stability and refolding of onconase
    Mandy Hacke
    Martin Luther University Halle Wittenberg, Institute of Biochemistry and Biotechnology, Germany
    FEBS J 280:4454-62. 2013
    ....
  14. ncbi Tandemization endows bovine pancreatic ribonuclease with cytotoxic activity
    Franziska Leich
    Department of Biochemistry and Biotechnology, Martin Luther University Halle Wittenberg, Kurt Mothes Str 3, 06120 Halle, Germany
    J Mol Biol 358:1305-13. 2006
    ..3-12.9 microM. These findings might establish the development of a new class of chemotherapeutic agents based on pancreatic ribonucleases...
  15. pmc Protein prosthesis: β-peptides as reverse-turn surrogates
    Ulrich Arnold
    Institute of Biochemistry and Biotechnology, Martin Luther University Halle Wittenberg, 06120 Halle, Germany
    Protein Sci 22:274-9. 2013
    ..These data indicate that hydrogen-bonding pattern merits careful consideration in the selection of beneficial reverse-turn surrogates...
  16. ncbi Proline versus charge concept for protein stabilization against proteolytic attack
    Yvonne Markert
    Department of Biochemistry and Biotechnology, Martin Luther Universitat Halle Wittenberg, Kurt Mothes Strasse 3, 06120 Halle, Germany
    Protein Eng 16:1041-6. 2003
    ....
  17. doi Striking stabilization of Rana catesbeiana ribonuclease 3 by guanidine hydrochloride
    Magali Solé
    Martin Luther University Halle Wittenberg, Institute of Biochemistry and Biotechnology, Kurt Mothes Str 3, 06120 Halle, Germany
    FEBS Lett 587:737-42. 2013
    ..This stabilizing effect was endorsed by in silico docking studies...
  18. ncbi Endocytotic internalization as a crucial factor for the cytotoxicity of ribonucleases
    Franziska Leich
    Institute of Biochemistry and Biotechnology, Martin Luther University Halle Wittenberg, Halle, Germany
    J Biol Chem 282:27640-6. 2007
    ....
  19. ncbi Identification of three phases in Onconase refolding
    Cindy Schulenburg
    Institute of Biochemistry and Biotechnology, Martin Luther University Halle Wittenberg, Halle, Germany
    FEBS J 274:5826-33. 2007
    ..e. in the last step of refolding. Evidently, in contrast to the folding of RNase A, there is no catalytically active native-like intermediate in the folding of Onconase...
  20. ncbi Protein prosthesis: a nonnatural residue accelerates folding and increases stability
    Ulrich Arnold
    Department of Biochemistry Biotechnology, Martin Luther University, 06099 Halle, Germany
    J Am Chem Soc 125:7500-1. 2003
    ..8 +/- 0.3 degrees C while having no effect on enzymatic activity. The rational use of this and other prosthetic segments could enable chemotherapeutic proteins to survive longer in vivo or retain activity after oral administration...
  21. ncbi The interdependence between catalytic activity, conformational stability, and cytotoxicity of onconase
    Cindy Schulenburg
    Martin Luther University Halle Wittenberg, Halle, Germany
    Cancer Biol Ther 6:1233-9. 2007
    ..Some cells were undergoing apoptosis. The cytotoxic and cytostatic effects of ONC and its variants were predominantly determined by their catalytic activity rather than by conformational stability...
  22. ncbi Semisynthesis of ribonuclease A using intein-mediated protein ligation
    Ulrich Arnold
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706, USA
    ScientificWorldJournal 2:1823-7. 2002
    ..These results might serve as basis for the semisynthesis of RNase A variants containing non-natural modules in the aforementioned peptide...
  23. ncbi Dissecting the effect of trifluoroethanol on ribonuclease A. Subtle structural changes detected by nonspecific proteases
    Jens Köditz
    Department of Biochemistry Biotechnology, Martin Luther University Halle Wittenberg, Halle, Germany
    Eur J Biochem 269:3831-7. 2002
    ..These changes, however, are too marginal to alter the overall catalytic and spectroscopic properties of ribonuclease A...
  24. pmc Replacing a single atom accelerates the folding of a protein and increases its thermostability
    Ulrich Arnold
    Institute of Biochemistry and Biotechnology, Martin Luther Universitat Halle Wittenberg, 06120 Halle, Germany
    Org Biomol Chem 14:6780-5. 2016
    ..Thus, an appropriately situated fluorine can serve as a prosthetic atom in the context of a protein. ..
  25. pmc Protein prosthesis: 1,5-disubstituted[1,2,3]triazoles as cis-peptide bond surrogates
    Annie Tam
    Department of Chemistry and Biochemistry, University of Wisconsin, Madison, WI 53706, USA
    J Am Chem Soc 129:12670-1. 2007