Rainer Lehmann

Summary

Country: Germany

Publications

  1. ncbi request reprint Simultaneous, quantitative analysis of UDP-N-acetylglucosamine, UDP-N-acetylgalactosamine, UDP-glucose and UDP-galactose in human peripheral blood cells, muscle biopsies and cultured mesangial cells by capillary zone electrophoresis
    R Lehmann
    Klinisch chemisches Zentrallabor der Universitätskliniken, Abteilung Innere Medizin IV, Tubingen, Germany
    Electrophoresis 21:3010-5. 2000
  2. pmc Medium chain acylcarnitines dominate the metabolite pattern in humans under moderate intensity exercise and support lipid oxidation
    Rainer Lehmann
    Central Laboratory, Division of Clinical Chemistry and Pathobiochemistry, University Hospital Tuebingen, Tuebingen, Germany
    PLoS ONE 5:e11519. 2010
  3. ncbi request reprint Investigation of a capillary electrophoretic approach for direct quantification of apolipoprotein A-I in serum
    Rainer Lehmann
    Klinisch chemisches Zentrallabor der Universitätskliniken, Abteilung Innere Medizin IV Universität Tübingen, Germany
    Electrophoresis 24:1422-8. 2003
  4. ncbi request reprint The phosphorylation of Ser318 of insulin receptor substrate 1 is not per se inhibitory in skeletal muscle cells but is necessary to trigger the attenuation of the insulin-stimulated signal
    Cora Weigert
    Department of Internal Medicine IV, University Hospital Tuebingen, Germany
    J Biol Chem 280:37393-9. 2005
  5. pmc Enforced expression of protein kinase C in skeletal muscle causes physical inactivity, fatty liver and insulin resistance in the brain
    Anita M Hennige
    University of Tuebingen, Department of Internal Medicine, Tubingen, Germany
    J Cell Mol Med 14:903-13. 2010
  6. doi request reprint Phosphorylation of Ser357 of rat insulin receptor substrate-1 mediates adverse effects of protein kinase C-delta on insulin action in skeletal muscle cells
    Rizwana Sanaullah Waraich
    Division of Clinical Chemistry and Pathobiochemistry, University of Tuebingen, Tuebingen, Germany
    J Biol Chem 283:11226-33. 2008
  7. doi request reprint Interplay and effects of temporal changes in the phosphorylation state of serine-302, -307, and -318 of insulin receptor substrate-1 on insulin action in skeletal muscle cells
    Cora Weigert
    Division of Clinical Chemistry and Pathobiochemistry, University Hospital of Tubingen, Tubingen, Germany
    Mol Endocrinol 22:2729-40. 2008
  8. pmc Phosphorylation of serine 1137/1138 of mouse insulin receptor substrate (IRS) 2 regulates cAMP-dependent binding to 14-3-3 proteins and IRS2 protein degradation
    Sabine S Neukamm
    Division of Clinical Chemistry and Pathobiochemistry, Department of Internal Medicine IV, University Hospital Tuebingen, Tuebingen 72076, Germany
    J Biol Chem 288:16403-15. 2013
  9. doi request reprint Development and precise characterization of phospho-site-specific antibody of Ser(357) of IRS-1: elimination of cross reactivity with adjacent Ser(358)
    Rizwana Sanaullah Waraich
    Department of Internal Medicine IV, Endocrinology, Metabolism, Pathobiochemistry and Clinical Chemistry, University Hospital Tuebingen, Germany
    Biochem Biophys Res Commun 376:26-31. 2008
  10. pmc Muscle and liver-specific alterations in lipid and acylcarnitine metabolism after a single bout of exercise in mice
    Miriam Hoene
    Division of Clinical Chemistry and Pathobiochemistry, Department of Diagnostic Laboratory Medicine, University Hospital Tuebingen, Tuebingen, Germany
    Sci Rep 6:22218. 2016

Collaborators

Detail Information

Publications39

  1. ncbi request reprint Simultaneous, quantitative analysis of UDP-N-acetylglucosamine, UDP-N-acetylgalactosamine, UDP-glucose and UDP-galactose in human peripheral blood cells, muscle biopsies and cultured mesangial cells by capillary zone electrophoresis
    R Lehmann
    Klinisch chemisches Zentrallabor der Universitätskliniken, Abteilung Innere Medizin IV, Tubingen, Germany
    Electrophoresis 21:3010-5. 2000
    ..The CZE method allows the study of the flux through the hexosamine pathway in diabetes mellitus and other diseases in a simple, quantitative and accurate way...
  2. pmc Medium chain acylcarnitines dominate the metabolite pattern in humans under moderate intensity exercise and support lipid oxidation
    Rainer Lehmann
    Central Laboratory, Division of Clinical Chemistry and Pathobiochemistry, University Hospital Tuebingen, Tuebingen, Germany
    PLoS ONE 5:e11519. 2010
    ..We aimed to identify and characterize metabolites, which are components of the regulatory network mediating the beneficial metabolic adaptation to exercise...
  3. ncbi request reprint Investigation of a capillary electrophoretic approach for direct quantification of apolipoprotein A-I in serum
    Rainer Lehmann
    Klinisch chemisches Zentrallabor der Universitätskliniken, Abteilung Innere Medizin IV Universität Tübingen, Germany
    Electrophoresis 24:1422-8. 2003
    ..Our study shows that CE is amenable to a fast analysis and a reproducible and reliable quantification of Apo A-I level in sera of various clinical samples...
  4. ncbi request reprint The phosphorylation of Ser318 of insulin receptor substrate 1 is not per se inhibitory in skeletal muscle cells but is necessary to trigger the attenuation of the insulin-stimulated signal
    Cora Weigert
    Department of Internal Medicine IV, University Hospital Tuebingen, Germany
    J Biol Chem 280:37393-9. 2005
    ..We conclude that Ser(318) phosphorylation of IRS-1 is an early physiological event in insulin-stimulated signal transduction, which attenuates the continuing action of insulin...
  5. pmc Enforced expression of protein kinase C in skeletal muscle causes physical inactivity, fatty liver and insulin resistance in the brain
    Anita M Hennige
    University of Tuebingen, Department of Internal Medicine, Tubingen, Germany
    J Cell Mol Med 14:903-13. 2010
    ....
  6. doi request reprint Phosphorylation of Ser357 of rat insulin receptor substrate-1 mediates adverse effects of protein kinase C-delta on insulin action in skeletal muscle cells
    Rizwana Sanaullah Waraich
    Division of Clinical Chemistry and Pathobiochemistry, University of Tuebingen, Tuebingen, Germany
    J Biol Chem 283:11226-33. 2008
    ..Together, these data suggest that phosphorylation of Ser(357) mediates at least in part the adverse effects of PKC-delta activation on insulin action...
  7. doi request reprint Interplay and effects of temporal changes in the phosphorylation state of serine-302, -307, and -318 of insulin receptor substrate-1 on insulin action in skeletal muscle cells
    Cora Weigert
    Division of Clinical Chemistry and Pathobiochemistry, University Hospital of Tubingen, Tubingen, Germany
    Mol Endocrinol 22:2729-40. 2008
    ..Thus we conclude that insulin stimulation temporally generates different phosphorylation statuses of the same residues that exert different functions in insulin signaling...
  8. pmc Phosphorylation of serine 1137/1138 of mouse insulin receptor substrate (IRS) 2 regulates cAMP-dependent binding to 14-3-3 proteins and IRS2 protein degradation
    Sabine S Neukamm
    Division of Clinical Chemistry and Pathobiochemistry, Department of Internal Medicine IV, University Hospital Tuebingen, Tuebingen 72076, Germany
    J Biol Chem 288:16403-15. 2013
    ..We present serine 1137/1138 as novel cAMP-dependent phosphorylation sites on IRS2 and show their importance in 14-3-3 binding and IRS2 protein stability...
  9. doi request reprint Development and precise characterization of phospho-site-specific antibody of Ser(357) of IRS-1: elimination of cross reactivity with adjacent Ser(358)
    Rizwana Sanaullah Waraich
    Department of Internal Medicine IV, Endocrinology, Metabolism, Pathobiochemistry and Clinical Chemistry, University Hospital Tuebingen, Germany
    Biochem Biophys Res Commun 376:26-31. 2008
    ..This antibody can be effectively used to further clarify the inhibitory role of Ser(357) in insulin signal transduction...
  10. pmc Muscle and liver-specific alterations in lipid and acylcarnitine metabolism after a single bout of exercise in mice
    Miriam Hoene
    Division of Clinical Chemistry and Pathobiochemistry, Department of Diagnostic Laboratory Medicine, University Hospital Tuebingen, Tuebingen, Germany
    Sci Rep 6:22218. 2016
    ..In addition, our results reveal that lysophospholipids and plasmalogens, potential lipid signalling molecules, are acutely regulated by physical exercise. ..
  11. pmc Purity matters: A workflow for the valid high-resolution lipid profiling of mitochondria from cell culture samples
    Lisa Kappler
    Division of Clinical Chemistry and Pathobiochemistry, Department of Diagnostic Laboratory Medicine, University Hospital Tuebingen, Tuebingen, Germany
    Sci Rep 6:21107. 2016
    ..To conclude, we present a protocol how to obtain reliable mitochondria-specific lipid profiles from cell culture samples and show that quality controls are indispensable when performing mitochondria lipidomics. ..
  12. doi request reprint The lipid profile of brown adipose tissue is sex-specific in mice
    Miriam Hoene
    Division of Clinical Chemistry and Pathobiochemistry, Department of Internal Medicine IV, University Hospital Tubingen, Tubingen, Germany Electronic address
    Biochim Biophys Acta 1842:1563-70. 2014
    ..These differences in phospholipid fatty acid composition could greatly affect mitochondrial membranes and other cellular organelles and thereby regulate the function of BAT in a sex-specific manner. ..
  13. pmc Circulating lysophosphatidylcholines are markers of a metabolically benign nonalcoholic fatty liver
    Rainer Lehmann
    Division of Endocrinology, Department of Internal Medicine, University Hospital Tubingen, Tubingen, Germany
    Diabetes Care 36:2331-8. 2013
    ..We investigated circulating markers and mechanisms of a metabolically benign and malignant NAFL by applying a metabolomic approach...
  14. pmc Clinical and non-targeted metabolomic profiling of homozygous carriers of Transcription Factor 7-like 2 variant rs7903146
    Robert Wagner
    1 Division of Endocrinology, Diabetology, Angiology and Nephrology, Department of Internal Medicine 4, University Hospital Tuebingen, Germany 2 Institute for Diabetes Research and Metabolic Diseases of the Helmholtz Zentrum München at the University of Tuebingen, Tuebingen, Germany 3 German Center for Diabetes Research DZD, Tübingen 4
    Sci Rep 4:5296. 2014
    ..Our study argues against a relevant impact of TCF7L2 rs7903146 on the systemic level in humans, but confirms the role in the pathogenesis of type 2 diabetes in humans as a mechanism impairing insulin secretion. ..
  15. ncbi request reprint How insulin receptor substrate proteins regulate the metabolic capacity of the liver--implications for health and disease
    Louise Fritsche
    Department of Internal Medicine 4, Division of Clinical Chemistry and Pathobiochemistry, Central Laboratory, University Hospital Tuebingen, Tuebingen, Germany
    Curr Med Chem 15:1316-29. 2008
    ....
  16. ncbi request reprint Insulin glulisine: insulin receptor signaling characteristics in vivo
    Anita M Hennige
    University of Tuebingen, Department of Internal Medicine IV, D 72076, Tuebingen, Germany
    Diabetes 54:361-6. 2005
    ..Moreover, insulin signaling in hypothalamic tissue determined by PI 3-kinase activity was comparable. Therefore, insulin glulisine may be a useful tool for diabetes treatment...
  17. doi request reprint Hepatic lipid composition and stearoyl-coenzyme A desaturase 1 mRNA expression can be estimated from plasma VLDL fatty acid ratios
    Andreas Peter
    Department of Internal Medicine, Division of Endocrinology, Diabetology, Vascular Medicine, Nephrology and Clinical Chemistry, University of Tubingen, Tubingen Germany
    Clin Chem 55:2113-20. 2009
    ..Additionally, we further evaluated commonly used fatty acid ratios [elongase, de novo lipogenesis, and Delta5 and Delta6 desaturase] in plasma VLDL and hepatic lipid fractions...
  18. ncbi request reprint Direct cross-talk of interleukin-6 and insulin signal transduction via insulin receptor substrate-1 in skeletal muscle cells
    Cora Weigert
    Department of Internal Medicine, Division of Endocrinology, Metabolism, Pathobiochemistry and Clinical Chemistry, University of Tubingen, Otfried Muller Strasse 10, D 72076 Germany
    J Biol Chem 281:7060-7. 2006
    ..Thus, the data provided evidence for a possible molecular mechanism of the physiological metabolic effects of IL-6 in skeletal muscle, thereby exerting short term beneficial effects on insulin action...
  19. doi request reprint Insulin-induced serine phosphorylation of IRS-2 via ERK1/2 and mTOR: studies on the function of Ser675 and Ser907
    Louise Fritsche
    Dept of Internal Medicine, Div of Pathobiochemistry and Clinical Chemistry, Univ of Tuebingen, Otfried Mueller Straße 10, 72076 Tuebingen, Germany
    Am J Physiol Endocrinol Metab 300:E824-36. 2011
    ....
  20. ncbi request reprint Novel hemoglobin variant [beta66(E10) Lys-->Asn], with decreased oxygen affinity, causes falsely low hemoglobin A1c values by HPLC
    Ulrich Friess
    Department of Medicine IV Endocrinology, Metabolism, and Clinical Chemistry, University of Tuebingen, Otfried Mueller Strasse 10, D 72076 Tuebingen, Germany
    Clin Chem 49:1412-5. 2003
  21. ncbi request reprint Liquid chromatography-based determination of urinary free and total N(epsilon)-(carboxymethyl)lysine excretion in normal and diabetic subjects
    Ulrich Friess
    Department of Medicine IV, University of Tuebingen, D 72076 Tuebingen, Germany
    J Chromatogr B Analyt Technol Biomed Life Sci 794:273-80. 2003
    ..The specific and sensitive determination of CML by RP-HPLC of its OPA-derivative is well suited for automation and better than that of less defined glycoxidation products (AGEs)...
  22. pmc The uncoordinated-5 homolog B (UNC5B) receptor increases myocardial ischemia-reperfusion injury
    David Kohler
    Department of Anesthesiology and Intensive Care Medicine, University Hospital, Tubingen, Germany
    PLoS ONE 8:e69477. 2013
    ..In summary our studies demonstrate an important role for UNC5B during myocardial IR injury, and that UNC5B might be a potential therapeutic target to control reperfusion injury in the future. ..
  23. pmc Identification of the amino acids 300-600 of IRS-2 as 14-3-3 binding region with the importance of IGF-1/insulin-regulated phosphorylation of Ser-573
    Sabine S Neukamm
    Division of Clinical Chemistry and Pathobiochemistry, Department of Internal Medicine IV, University Hospital Tuebingen, Tuebingen, Germany
    PLoS ONE 7:e43296. 2012
    ..To conclude, our data suggest a physiologically relevant role for IGF-1/insulin-dependent 14-3-3 binding to IRS-2 involving serine 573...
  24. pmc Metabolic signatures of cultured human adipocytes from metabolically healthy versus unhealthy obese individuals
    Anja Böhm
    Helmholtz Zentrum Munchen, German Research Center for Environmental Health, Institute of Experimental Genetics, Genome Analysis Center, Neuherberg, Germany Department of Internal Medicine IV, Division of Endocrinology, Diabetology, Angiology, Nephrology, and Clinical Chemistry, University Hospital, Eberhard Karls University, Tubingen, Germany German Center for Diabetes Research, Neuherberg, Germany Institute for Diabetes Research and Metabolic Diseases of the Helmholtz Center München at the Eberhard Karls University of Tübingen, Tubingen, Germany
    PLoS ONE 9:e93148. 2014
    ..Aim of this study was, to identify adipocyte-specific metabolic signatures and functional biomarkers for MHO versus MUHO...
  25. ncbi request reprint Identification of an in vitro insulin receptor substrate-1 phosphorylation site by negative-ion muLC/ES-API-CID-MS hybrid scan technique
    Alexander Beck
    Department of Internal Medicine IV, Division of Clinical Chemistry, University Hospital Tuebingen, Germany
    J Am Soc Mass Spectrom 14:401-5. 2003
    ..Together, our results indicate that the applied strategy is useful for unequivocal and fast analysis of phosphorylation sites in low abundant signaling proteins...
  26. doi request reprint Phosphorylation of vasodilator-stimulated phosphoprotein prevents platelet-neutrophil complex formation and dampens myocardial ischemia-reperfusion injury
    David Kohler
    Department of Anesthesiology and Intensive Care Medicine, University Hospital, Tubingen, Germany
    Circulation 123:2579-90. 2011
    ..Given the importance of vasodilator-stimulated phosphoprotein (VASP) for platelet function, we pursued the role of VASP on the formation of PNCs and its impact on the extent of myocardial ischemia-reperfusion (IR) injury...
  27. doi request reprint The secretome of the working human skeletal muscle--a promising opportunity to combat the metabolic disaster?
    Cora Weigert
    Division of Endocrinology, Diabetology, Angiology, Nephrology, Pathobiochemistry and Clinical Chemistry, Department of Internal Medicine, University of Tuebingen, Tuebingen, Germany Institute for Diabetes Research and Metabolic Diseases of the Helmholtz Zentrum Muenchen, University of Tuebingen, Tuebingen, Germany German Center for Diabetes Research DZD, Neuherberg, Germany
    Proteomics Clin Appl 8:5-18. 2014
    ..The identification of these metabolites will lead to a more comprehensive view described by a new myo(metabo)kinome consisting of peptides, proteins, and metabolites. ..
  28. ncbi request reprint Cathepsin G, and not the asparagine-specific endoprotease, controls the processing of myelin basic protein in lysosomes from human B lymphocytes
    Timo Burster
    Department of Medicine II, University of Tubingen, Tubingen, Germany
    J Immunol 172:5495-503. 2004
    ..Internalization of exogenous CatG represents a novel mechanism of professional APC to acquire functionally dominant proteolytic activity that complements the panel of endogenous lysosomal enzymes...
  29. doi request reprint Hypoxia-inducible factor-1 is central to cardioprotection: a new paradigm for ischemic preconditioning
    Tobias Eckle
    Department of Anesthesiology and Intensive Care Medicine, University Hospital, Tubingen, Germany
    Circulation 118:166-75. 2008
    ..Convincing evidence confirms a central role of hypoxia-inducible factor (HIF)-1 in mammalian oxygen homeostasis. Thus, we pursued HIF-1 as a central component of cardioprotection by ischemic preconditioning...
  30. ncbi request reprint Insulin-induced stimulation of JNK and the PI 3-kinase/mTOR pathway leads to phosphorylation of serine 318 of IRS-1 in C2C12 myotubes
    Karsten Müssig
    Division of Endocrinology, Metabolism and Pathobiochemistry, Department of Internal Medicine, University Hospital of Tubingen, 72076 Tubingen, Germany
    Biochem Biophys Res Commun 335:819-25. 2005
    ....
  31. ncbi request reprint Point-counterpoint: Interleukin-6 does/does not have a beneficial role in insulin sensitivity and glucose homeostasis
    Cora Weigert
    J Appl Physiol 102:820-1; author reply 825. 2007
  32. ncbi request reprint Protein kinase C-zeta-induced phosphorylation of Ser318 in insulin receptor substrate-1 (IRS-1) attenuates the interaction with the insulin receptor and the tyrosine phosphorylation of IRS-1
    Klaus Moeschel
    Department of Internal Medicine IV, Endocrinology, Metabolism, Pathobiochemistry and Clinical Chemistry, University Hospital Tubingen, D 72076 Tubingen, Germany
    J Biol Chem 279:25157-63. 2004
    ..These results, generated in BHK(IR) cells, suggest that phosphorylation of Ser(318) by PKC-zeta might contribute to the inhibitory effect of prolonged hyperinsulinemia on IRS-1 function...
  33. ncbi request reprint Partitioning-defective protein 6 regulates insulin-dependent glycogen synthesis via atypical protein kinase C
    Peter Weyrich
    Medical Clinic IV, Otfried Muller Strasse 10, 72076 Tubingen, Germany
    Mol Endocrinol 18:1287-300. 2004
    ..Consequently, insulin-dependent glycogen synthesis was down-regulated (1.44 vs. 2.24 fold, P < 0.01). We therefore suggest that Par6 acts as a negative regulator of the insulin signal...
  34. ncbi request reprint Leptin down-regulates insulin action through phosphorylation of serine-318 in insulin receptor substrate 1
    Anita M Hennige
    University of Tuebingen, Department of Internal Medicine IV, Tuebingen D 72076, Germany
    FASEB J 20:1206-8. 2006
    ..In summary, these data suggest that phosphorylation of Ser-318 in Irs1 mediates the inhibitory signal of leptin on the insulin-signaling cascade in obese subjects...
  35. ncbi request reprint Evidence for a novel TGF-beta1-independent mechanism of fibronectin production in mesangial cells overexpressing glucose transporters
    Cora Weigert
    Department of Internal Medicine, Division of Endocrinology, Metabolism and Pathobio Chemistry, University of Tubingen, Germany
    Diabetes 52:527-35. 2003
    ..Our data indicate that increased glucose uptake and metabolism induce PKC-dependent AP-1 activation that is sufficient for enhanced fibronectin production, but not for increased TGF-beta1 expression...
  36. doi request reprint Novel, fully automatic hydrophilic interaction/reversed-phase column-switching high-performance liquid chromatographic system for the complementary analysis of polar and apolar compounds in complex samples
    Yuan Wang
    National Chromatographic R and A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, China
    J Chromatogr A 1204:28-34. 2008
    ....
  37. doi request reprint Metabonomics study on the effects of the ginsenoside Rg3 in a beta-cyclodextrin-based formulation on tumor-bearing rats by a fully automatic hydrophilic interaction/reversed-phase column-switching HPLC-ESI-MS approach
    Yuan Wang
    National Chromatography Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 16023 Dalian, China
    Anal Chem 80:4680-8. 2008
    ..It may not only increase the number of discovered biomarkers but consequently improve the comprehensive information on metabolic changes in a fully automatic manner...
  38. ncbi request reprint Evaluation of stress responses to interval training at low and moderate altitudes
    Andreas Michael Niess
    Center of Internal Medicine, Department of Rehabilitative and Preventive Sports Medicine, University of Freiburg, Freiburg, Germany
    Med Sci Sports Exerc 35:263-9. 2003
    ....
  39. doi request reprint Practical approach for the identification and isomer elucidation of biomarkers detected in a metabonomic study for the discovery of individuals at risk for diabetes by integrating the chromatographic and mass spectrometric information
    Jing Chen
    National Chromatographic R and A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China
    Anal Chem 80:1280-9. 2008
    ..The proposed strategy is a powerful new analytical tool, which will allow the definite identification of physiologically important molecules in metabonomics studies from basic biochemistry to clinical biomarker discovery...