H Wagner

Summary

Affiliation: Klinikum rechts der Isar
Country: Germany

Publications

  1. ncbi request reprint The immunobiology of the TLR9 subfamily
    Hermann Wagner
    Institute of Medical Microbiology, Immunology and Hygiene, Trogerstr 9, 81675 Munich, Germany
    Trends Immunol 25:381-6. 2004
  2. ncbi request reprint Interactions between bacterial CpG-DNA and TLR9 bridge innate and adaptive immunity
    Hermann Wagner
    Institute of Medical Microbiology, Immunology and Hygiene, Technische Universitat Munchen, Trogerstrasse 9, 81675 Munich, Germany
    Curr Opin Microbiol 5:62-9. 2002
  3. pmc All is not Toll: new pathways in DNA recognition
    Hermann Wagner
    Institute of Medical Microbiology, Munchen, Germany
    J Exp Med 203:265-8. 2006
  4. ncbi request reprint Targeting split vaccines to the endosome improves vaccination
    Hermann Wagner
    Institute of Medical Microbiology, Immunology and Hygiene, Trogerstrasse 9, 81675 Munich, Germany
    Curr Opin Biotechnol 15:538-42. 2004
  5. pmc Immune cell activation by bacterial CpG-DNA through myeloid differentiation marker 88 and tumor necrosis factor receptor-associated factor (TRAF)6
    H Hacker
    Institute of Medical Microbiology, Immunology and Hygiene, Technische Universitat Munchen, D 81675 Munich, Germany
    J Exp Med 192:595-600. 2000
  6. ncbi request reprint CpG-containing synthetic oligonucleotides promote B and cytotoxic T cell responses to protein antigen: a new class of vaccine adjuvants
    G B Lipford
    Institute for Medical Microbiology, Immunology and Hygiene, Technical University of Munich, Germany
    Eur J Immunol 27:2340-4. 1997
  7. ncbi request reprint Bacterial CpG-DNA and lipopolysaccharides activate Toll-like receptors at distinct cellular compartments
    Parviz Ahmad-Nejad
    Institute of Medical Microbiology, Immunology and Hygiene, Technische Universitat Munchen, Munich, Germany
    Eur J Immunol 32:1958-68. 2002
  8. ncbi request reprint Endocytosed HSP60s use toll-like receptor 2 (TLR2) and TLR4 to activate the toll/interleukin-1 receptor signaling pathway in innate immune cells
    R M Vabulas
    Institute of Medical Microbiology, Immunology and Hygiene, Technical University of Munich, Trogerstr. 9, 81675 Munich, Germany
    J Biol Chem 276:31332-9. 2001
  9. ncbi request reprint Bacterial CpG-DNA licenses TLR9
    S Bauer
    Institute for Medical Microbiology, Hygiene and Immunology, Trogerstrasse 9, 81675 Munich, Germany
    Curr Top Microbiol Immunol 270:145-54. 2002
  10. ncbi request reprint Bacterial CpG-DNA triggers activation and maturation of human CD11c-, CD123+ dendritic cells
    M Bauer
    Institute for Medical Microbiology, Immunology and Hygiene, Klinikum rechts der Isar, Technical University of Munich, Munich, Germany
    J Immunol 166:5000-7. 2001

Collaborators

Detail Information

Publications95

  1. ncbi request reprint The immunobiology of the TLR9 subfamily
    Hermann Wagner
    Institute of Medical Microbiology, Immunology and Hygiene, Trogerstr 9, 81675 Munich, Germany
    Trends Immunol 25:381-6. 2004
  2. ncbi request reprint Interactions between bacterial CpG-DNA and TLR9 bridge innate and adaptive immunity
    Hermann Wagner
    Institute of Medical Microbiology, Immunology and Hygiene, Technische Universitat Munchen, Trogerstrasse 9, 81675 Munich, Germany
    Curr Opin Microbiol 5:62-9. 2002
    ..Thus, interactions between CpG-DNA and TLR9 effectively bridge innate and acquired immunity...
  3. pmc All is not Toll: new pathways in DNA recognition
    Hermann Wagner
    Institute of Medical Microbiology, Munchen, Germany
    J Exp Med 203:265-8. 2006
    ..In addition, recent studies have revealed the existence of TLR-independent pathways that are activated in response to microbial and host nucleic acids...
  4. ncbi request reprint Targeting split vaccines to the endosome improves vaccination
    Hermann Wagner
    Institute of Medical Microbiology, Immunology and Hygiene, Trogerstrasse 9, 81675 Munich, Germany
    Curr Opin Biotechnol 15:538-42. 2004
    ....
  5. pmc Immune cell activation by bacterial CpG-DNA through myeloid differentiation marker 88 and tumor necrosis factor receptor-associated factor (TRAF)6
    H Hacker
    Institute of Medical Microbiology, Immunology and Hygiene, Technische Universitat Munchen, D 81675 Munich, Germany
    J Exp Med 192:595-600. 2000
    ..Activation of the TLR/IL-1R signaling pathway by foreign bacterial DNA may be one way to initiate innate defense mechanisms against infectious pathogens in vivo...
  6. ncbi request reprint CpG-containing synthetic oligonucleotides promote B and cytotoxic T cell responses to protein antigen: a new class of vaccine adjuvants
    G B Lipford
    Institute for Medical Microbiology, Immunology and Hygiene, Technical University of Munich, Germany
    Eur J Immunol 27:2340-4. 1997
    ..Thus ssDNA oligomers may serve as inexpensive and safe vaccine adjuvants and, in addition, differential effects due to sequence may allow for directed responses...
  7. ncbi request reprint Bacterial CpG-DNA and lipopolysaccharides activate Toll-like receptors at distinct cellular compartments
    Parviz Ahmad-Nejad
    Institute of Medical Microbiology, Immunology and Hygiene, Technische Universitat Munchen, Munich, Germany
    Eur J Immunol 32:1958-68. 2002
    ..These data stress the need to characterize individual TLR at the very beginning of signal initiation in order to understand their diverse biological functions...
  8. ncbi request reprint Endocytosed HSP60s use toll-like receptor 2 (TLR2) and TLR4 to activate the toll/interleukin-1 receptor signaling pathway in innate immune cells
    R M Vabulas
    Institute of Medical Microbiology, Immunology and Hygiene, Technical University of Munich, Trogerstr. 9, 81675 Munich, Germany
    J Biol Chem 276:31332-9. 2001
    ..Initiation of signaling requires endocytosis of HSP60 that is effectively inhibited by serum component(s). The results revealed that adjuvanticity of HSP60 operates similar to that of classical pathogen-derived ligands...
  9. ncbi request reprint Bacterial CpG-DNA licenses TLR9
    S Bauer
    Institute for Medical Microbiology, Hygiene and Immunology, Trogerstrasse 9, 81675 Munich, Germany
    Curr Top Microbiol Immunol 270:145-54. 2002
    ..This review focuses on TLR9 which is an essential component for the recognition of bacterial CpG-DNA. Expression of TLR9 and structural consideration as well as direct ligand interaction of TLR9 and CpG-DNA are discussed...
  10. ncbi request reprint Bacterial CpG-DNA triggers activation and maturation of human CD11c-, CD123+ dendritic cells
    M Bauer
    Institute for Medical Microbiology, Immunology and Hygiene, Klinikum rechts der Isar, Technical University of Munich, Munich, Germany
    J Immunol 166:5000-7. 2001
    ..Taken together, these results suggest that within a DC subset a multiplicity of responses can be generated by distinct environmental stimuli and that responses to a given stimulus may be dissimilar between DC subsets...
  11. ncbi request reprint Guanosine-rich oligodeoxynucleotides induce proliferation of macrophage progenitors in cultures of murine bone marrow cells
    R Lang
    Institute of Medical Microbiology, Technical University of Munich, Munich, Germany
    Eur J Immunol 29:3496-506. 1999
    ..As such, these data reveal a so far unrecognized potential of G-rich ODN to signal directly outgrowth of macrophage progenitors from BMC...
  12. doi request reprint The immunogenicity of CpG-antigen conjugates
    Hermann Wagner
    Institut fur Medizinische Mikrobiologie, Immunologie und Hygiene, Technische Universitat Munchen, Germany
    Adv Drug Deliv Rev 61:243-7. 2009
    ..Furthermore, our data suggest that functional inactivation of Foxp3(+) regulatory T cells further enhances the immunogenicity of "split vaccines"...
  13. ncbi request reprint Heat shock protein 60 from Chlamydia pneumoniae elicits an unusual set of inflammatory responses via Toll-like receptor 2 and 4 in vivo
    Clarissa U Prazeres Da Costa
    Institute of Medical Microbiology, Immunology and Hygiene, Technical University of Munich, Munich, Germany
    Eur J Immunol 34:2874-84. 2004
    ..In conclusion, HSP60 from C. pneumoniae triggers inflammatory responses in vivo that differ from responses induced by endotoxin or CpG oligonucleotides and are dependent on TLR2 and 4...
  14. ncbi request reprint Endosomal translocation of vertebrate DNA activates dendritic cells via TLR9-dependent and -independent pathways
    Kei Yasuda
    Institute of Medical Microbiology, Immunology, and Hygiene, Technical University of Munich, Munich, Germany
    J Immunol 174:6129-36. 2005
    ..Endosomal compartmentalization of TLR9 may thus reflect an evolutionary strategy to avoid TLR9 activation by self-DNA...
  15. pmc Herpes simplex virus type-1 induces IFN-alpha production via Toll-like receptor 9-dependent and -independent pathways
    Hubertus Hochrein
    Institute of Medical Microbiology, Immunology, and Hygiene, Technical University of Munich, 81675 Munich, Germany
    Proc Natl Acad Sci U S A 101:11416-21. 2004
    ..Heat treatment of HSV-1 abolished maturation and IFN-alpha production from all TLR9-deficient DC but not WT DC. Thus pDC and non-pDC produce IFN-alpha in response to HSV-1 via both TLR9-independent and -dependent pathways...
  16. ncbi request reprint Transcriptional activation induced in macrophages by Toll-like receptor (TLR) ligands: from expression profiling to a model of TLR signaling
    Frank Schmitz
    Institute of Medical Microbiology, Immunology and Hygiene, Technische Universitat Munchen, Munich, Germany
    Eur J Immunol 34:2863-73. 2004
    ..We conclude with a TLR signaling model that incorporates both negative and synergistic interactions of MyD88- and TRIF-controlled signaling pathways as deduced from the microarray data presented...
  17. ncbi request reprint Species-specific recognition of single-stranded RNA via toll-like receptor 7 and 8
    Florian Heil
    Institute of Medical Microbiology, Immunology and Hygiene, Technische Universitat Munchen, Trogerstr 9, D 81675 Munich, Germany
    Science 303:1526-9. 2004
    ..These data suggest that ssRNA represents a physiological ligand for TLR7 and TLR8...
  18. ncbi request reprint Cutting edge: Toll-like receptor 9 expression is not required for CpG DNA-aided cross-presentation of DNA-conjugated antigens but essential for cross-priming of CD8 T cells
    Antje Heit
    Institute of Medical Microbiology, Immunology and Hygiene, Technische Universitat Munchen, Munich, Germany
    J Immunol 170:2802-5. 2003
    ..These findings imply that TLR9 does not trigger endocytosis of CpG-OVA conjugates, but activates DCs downstream of endocytosis...
  19. ncbi request reprint Heat shock proteins as ligands of toll-like receptors
    R M Vabulas
    Institute for Medical Microbiology, Immunology and Hygiene, Technical University of Munich, Trogerstr 4a, 81675 Munich, Germany
    Curr Top Microbiol Immunol 270:169-84. 2002
    ..Understanding the mechanisms of innate immune system interaction with HSPs will make it possible to rationally modulate immune responses, either towards immunity or towards tolerance...
  20. ncbi request reprint Extracting oscillations. Neuronal coincidence detection with noisy periodic spike input
    R Kempter
    Theoretische Physik, Physik Department, Munchen, James Franck Strasse, D 85747, DE
    Neural Comput 10:1987-2017. 1998
    ..Our considerations can be used to predict from neuronal parameters whether and to what extent a neuron can act as a coincidence detector and thus can convert a temporal code into a rate code...
  21. ncbi request reprint Vaccination with plasmid DNA activates dendritic cells via Toll-like receptor 9 (TLR9) but functions in TLR9-deficient mice
    Barbara Spies
    Institute of Medical Microbiology, Immunology, and Hygiene, Technische Universitat Munchen, Munich, Germany
    J Immunol 171:5908-12. 2003
    ..Overall, these results negate a dominant role of CpG-DNA/TLR9 interactions in long-term vaccination protocols...
  22. ncbi request reprint Cutting edge: myeloid differentiation factor 88 deficiency improves resistance against sepsis caused by polymicrobial infection
    Heike Weighardt
    Department of Surgery, Klinikum rechts der Isar, Technische Universitat Munchen, Munich, Germany
    J Immunol 169:2823-7. 2002
    ..These results imply a central role of MyD88 for the systemic immune pathology of polymicrobial sepsis and show that cytokine production in spleen and induction of certain chemokines are MyD88 independent...
  23. ncbi request reprint Role of chlamydial heat shock protein 60 in the stimulation of innate immune cells by Chlamydia pneumoniae
    Clarissa Prazeres da Costa
    Institute of Medical Microbiology, Immunology and Hygiene, Technical University of Munich, Germany
    Eur J Immunol 32:2460-70. 2002
    ..pneumoniae stimulated BMDDC in a TLR2- and TLR4-dependent fashion similar to the whole microorganism. In summary, these data suggest chlamydial HSP60 as an important mediator of inflammatory responses during infection with C. pneumoniae...
  24. ncbi request reprint CpG-DNA-mediated transient lymphadenopathy is associated with a state of Th1 predisposition to antigen-driven responses
    G B Lipford
    Institute for Medical Microbiology, Immunology and Hygiene, Klinikum rechts der Isar, Technical University of Munich, Munich, Germany
    J Immunol 165:1228-35. 2000
    ..The induction by the innate immune system of a locally contained hypersensitivity could represent a capacitating immune reaction yielding rapid conditioned responses to secondary infections...
  25. pmc Human TLR9 confers responsiveness to bacterial DNA via species-specific CpG motif recognition
    S Bauer
    Institute for Medical Microbiology, Immunology and Hygiene, Technical University of Munich, 81675 Munich, Germany
    Proc Natl Acad Sci U S A 98:9237-42. 2001
    ..The optimal CpG motif for hTLR9 was GTCGTT, whereas the optimal murine sequence was GACGTT. Overall, these data suggest that hTLR9 conveys CpG-DNA responsiveness to human cells by directly engaging immunostimulating CpG-DNA...
  26. ncbi request reprint The Gram-negative bacterium Chlamydia trachomatis L2 stimulates tumor necrosis factor secretion by innate immune cells independently of its endotoxin
    Sigrid Prebeck
    Institute of Medical Microbiology, Immunology and Hygiene, Technical University of Munich, Trogerstr 9, 81675 Munich, Germany
    Microbes Infect 5:463-70. 2003
    ..coli O55:B5 endotoxin on all cell types tested. We conclude that chlamydial endotoxin is unlikely to be involved in C. trachomatis L(2)-induced release of TNF by innate immune cells...
  27. doi request reprint The sweetness of the DNA backbone drives Toll-like receptor 9
    Hermann Wagner
    Institut fur Medizinische Mikrobiologie, Immunologie und Hygiene, Technische Universitat Munchen, Trogerstr 30, Munchen, Germany
    Curr Opin Immunol 20:396-400. 2008
    ..Thus evolutionary pressure might have exiled nucleic acid recognizing TLRs such as TLR9 to endosomes in order to avoid activation by host (self) derived nucleic acids...
  28. ncbi request reprint Treatment of Chlamydia pneumoniae infection with roxithromycin and effect on neointima proliferation after coronary stent placement (ISAR-3): a randomised, double-blind, placebo-controlled trial
    F Neumann
    Medizinische Klinik, Technische Universitat Munchen, 81675, Munchen, Germany
    Lancet 357:2085-9. 2001
    ..Our aim was to investigate whether or not treatment of C pneumoniae infection with antibiotics prevents restenosis after coronary stent placement...
  29. ncbi request reprint Functional evaluation of HIV/SIV Nef as superantigen
    M S Lapatschek
    Institute of Medical Microbiology, Immunology, and Hygiene, Technical University of Munich, Trogerstrasse 9, Munich, 81675, Germany
    Virology 282:329-37. 2001
    ..Therefore, we cannot support the hypothesis that Nef is a superantigen. The observations in favor of that hypothesis must be explained by other mechanisms...
  30. ncbi request reprint CpG motif-independent activation of TLR9 upon endosomal translocation of "natural" phosphodiester DNA
    Kei Yasuda
    Institut für Med Mikrobiologie, Immunologie und Hygiene, Technische Universitat Munchen, Munchen, 81675 Munich, Germany
    Eur J Immunol 36:431-6. 2006
    ..These results imply that both the chemical modification of the DNA backbone as well as the amount of endosomally translocated DNA represent determining factors that allow CpG motif-independent activation of TLR9 by ss PD-DNA...
  31. pmc Antagonistic antibody prevents toll-like receptor 2-driven lethal shock-like syndromes
    Guangxun Meng
    Institute of Medical Microbiology, Immunology, and Hygiene, Technical University of Munich, Germany
    J Clin Invest 113:1473-81. 2004
    ..These results indicate that epitope-specific binding of exogenous ligands precedes specific TLR signaling and suggest therapeutic application of a neutralizing anti-TLR2 antibody in acute infection...
  32. ncbi request reprint Protective CD8 T cell immunity triggered by CpG-protein conjugates competes with the efficacy of live vaccines
    Antje Heit
    Institute of Medical Microbiology, Immunology and Hygiene, Technische Universitat Munchen, Germany
    J Immunol 174:4373-80. 2005
    ..These data underscore that the synergy imparted by CpG-OVA complex-mediated combined triggering of innate and specific immunity might be key to initiate CD8 T cell-based immunoprotection by synthetic vaccines based on subunit Ag...
  33. ncbi request reprint CpG-DNA aided cross-priming by cross-presenting B cells
    Antje Heit
    Institute of Medical Microbiology, Immunology and Hygiene, Technical University Munich, Munich, Germany
    J Immunol 172:1501-7. 2004
    ..Overall, these findings disclose attributes of B cells, including cross-presentation of exogenous Ag and cross-priming of naive CD8 T cells that hitherto have been considered as hallmarks restricted to DCs...
  34. ncbi request reprint Lack of antigen-specific Th1 response alters granuloma formation and composition in Schistosoma mansoni-infected MyD88-/- mice
    Laura E Layland
    Institute of Medical Microbiology, Immunology and Hygiene, Technical University of Munich, Munich, Germany
    Eur J Immunol 35:3248-57. 2005
    ..Thus, MyD88 plays an important role in cellular infiltration, granuloma composition and T cell responses during schistosomiasis...
  35. ncbi request reprint Polymorphonuclear neutrophils improve replication of Chlamydia pneumoniae in vivo upon MyD88-dependent attraction
    Nuria Rodriguez
    Institute of Medical Microbiology, Immunology and Hygiene, Technical University of Munich, Munich, Germany
    J Immunol 174:4836-44. 2005
    ..pneumoniae in epithelial cells. Thus, the lower chlamydial burden in MyD88-deficient mice can be explained by the failure to recruit PMN...
  36. ncbi request reprint Antigen co-encapsulated with adjuvants efficiently drive protective T cell immunity
    Antje Heit
    Institute for Medical Microbiology, Immunology and Hygiene, Technical University of Munich, Munich, Germany
    Eur J Immunol 37:2063-74. 2007
    ....
  37. pmc Sequence independent interferon-alpha induction by multimerized phosphodiester DNA depends on spatial regulation of Toll-like receptor-9 activation in plasmacytoid dendritic cells
    Tobias Haas
    Institut fur Medizinische Mikrobiologie, Immunologie und Hygiene, Technische Universitat Munchen, Trogerstrasse, Munich, Germany
    Immunology 126:290-8. 2009
    ....
  38. doi request reprint Circumvention of regulatory CD4(+) T cell activity during cross-priming strongly enhances T cell-mediated immunity
    Antje Heit
    Institute for Medical Microbiology, Immunology and Hygiene, Technical University Munich, Munich, Germany
    Eur J Immunol 38:1585-97. 2008
    ..These findings have important implications for the improvement of vaccines against intracellular pathogens or tumors, especially in patients with highly active Treg...
  39. ncbi request reprint HSP70 as endogenous stimulus of the Toll/interleukin-1 receptor signal pathway
    Ramunas M Vabulas
    Institute of Medical Microbiology, Immunology and Hygiene, Technical University of Munich, Trogerstrasse 9, Munich 81675, Germany
    J Biol Chem 277:15107-12. 2002
    ....
  40. ncbi request reprint Cellular recognition of tri-/di-palmitoylated peptides is independent from a domain encompassing the N-terminal seven leucine-rich repeat (LRR)/LRR-like motifs of TLR2
    Guangxun Meng
    Institute of Medical Microbiology, Immunology, and Hygiene, Technical University of Munich, D 81675 Munich, Germany
    J Biol Chem 278:39822-9. 2003
    ..They further imply that TLR2 contains multiple binding domains for ligands that may contribute to the characterization of its promiscuous molecular pattern recognition...
  41. doi request reprint The DNA sugar backbone 2' deoxyribose determines toll-like receptor 9 activation
    Tobias Haas
    Institut fur Medizinische Mikrobiologie, Immunologie und Hygiene, Technische Universitat Munchen, Trogerstrasse 30, 81675 Munchen, Germany
    Immunity 28:315-23. 2008
    ....
  42. pmc Adjuvanticity of a synthetic cord factor analogue for subunit Mycobacterium tuberculosis vaccination requires FcRgamma-Syk-Card9-dependent innate immune activation
    Kerstin Werninghaus
    Institute of Medical Microbiology, Immunology and Hygiene, Technical University Munich, D 81675 Munich, Germany
    J Exp Med 206:89-97. 2009
    ..These data provide a molecular basis for the immunostimulatory activity of TDB and TDM and identify the Syk-Card9 pathway as a rational target for vaccine development against tuberculosis...
  43. pmc Lack of host gut microbiota alters immune responses and intestinal granuloma formation during schistosomiasis
    M Holzscheiter
    Institut fur Medizinische Mikrobiologie, Immunologie und Hygiene MIH, Technische Universitat Munchen, Munchen, Germany
    Clin Exp Immunol 175:246-57. 2014
    ..In addition, schistosome-specific immune responses were skewed and faecal egg excretion was diminished. This study demonstrates that host microbiota can act as a third partner in instigating helminth-specific immune responses. ..
  44. ncbi request reprint Plant-derived acetophenones with antiasthmatic and anti-inflammatory properties: inhibitory effects on chemotaxis, right angle light scatter and actin polymerization of polymorphonuclear granulocytes
    A A Müller
    Institut fur Pharmazie, Lehrstuhl fur Pharmazeutische Biologie, Ludwig Maximilians Universitat, Munchen, Germany
    Planta Med 65:590-4. 1999
    ..Acetosyringenin (2) was shown to reduce mastoparan-induced PMN migration. The inhibition of receptor independent motility of PMN suggests that the active metabolites of acetophenones may interfere with the post receptor signalling...
  45. ncbi request reprint Toll-like receptor 9 contributes to recognition of Mycobacterium bovis Bacillus Calmette-Guérin by Flt3-ligand generated dendritic cells
    Ferdinand von Meyenn
    Institut fur Medizinische Mikrobiologie, Immunologie and Hygiene, Technische Universitat Munchen, Trogerstrasse 30, 81675 Munchen, Germany
    Immunobiology 211:557-65. 2006
    ..Similarly, up-regulation of CD86 is abolished only in TLR2/4/9-deficient DCs supporting a role of TLR9 in the recognition of M. bovis BCG by murine dendritic cells...
  46. ncbi request reprint CpG-DNA aided cross-presentation of soluble antigens by dendritic cells
    Tobias Maurer
    Institute of Medical Microbiology, Immunology and Hygiene, Technical University of Munich, Munich, Germany
    Eur J Immunol 32:2356-64. 2002
    ..Our studies show that stimulatory CpG-ODN linked to Ag fulfill a dual role: enhancement of Ag uptake yielding efficient Ag cross-presentation by DC and in addition, their activation into professional DC...
  47. ncbi request reprint Human but not murine toll-like receptor 2 discriminates between tri-palmitoylated and tri-lauroylated peptides
    Alina Grabiec
    Institute of Medical Microbiology, Immunology, and Hygiene, Technical University of Munich, D 81675 Munich, Germany
    J Biol Chem 279:48004-12. 2004
    ..Despite similar domain structure of both TLR2 molecules, species-specific properties thus exist. Our results imply distinct susceptibilities of humans and mice to challenge with specific TLR2 ligands...
  48. ncbi request reprint Toll-like receptor 9 signaling can sensitize fibroblasts for apoptosis
    Silke F Fischer
    Institute for Medical Microbiology, Immunology and Hygiene, Technische Universitat Munchen, Trogerstr 9, D 81675 Munich, Germany
    Immunol Lett 97:115-22. 2005
    ..These results identify a mitochondrial pathway to apoptosis that can be triggered by TLR9 and that may serve to sensitize cells from the innate immune system to apoptosis in the course of an immune response...
  49. ncbi request reprint Contribution of toll-like receptors 2 and 4 in an oral Yersinia enterocolitica mouse infection model
    Andreas Sing
    Max von Pettenkofer Institut fur Hygiene und Medizinische Mikrobiologie, Ludwig Maximilians Universitat Munchen, Munchen, Germany
    Int J Med Microbiol 293:341-8. 2003
    ..enterocolitica infection than wild-type C3H mice. To our knowledge, this is the first report on a divergent influence of a TLR-deficiency on infection outcome in mice of different genetic backgrounds...
  50. pmc The NF-kappaB regulator Bcl-3 and the BH3-only proteins Bim and Puma control the death of activated T cells
    Anette Bauer
    Institute for Medical Microbiology, Technical University Munich, Trogerstrasse 9, D 81675 Munich, Germany
    Proc Natl Acad Sci U S A 103:10979-84. 2006
    ..IL-1, IL-7, and IL-15 primed T cells for survival even in the absence of Bim or Puma. Our data define interrelations and a Bim-independent pathway to activated T cell death...
  51. ncbi request reprint Decreased pathology and prolonged survival of human DC-SIGN transgenic mice during mycobacterial infection
    Martin Schaefer
    Institute for Medical Microbiology, Immunology, and Hygiene, Technische Universitat Munchen, Munich, Germany
    J Immunol 180:6836-45. 2008
    ..Based on our in vivo data, we propose that instead of favoring the immune evasion of mycobacteria, human DC-SIGN may have evolved as a pathogen receptor promoting protection by limiting tuberculosis-induced pathology...
  52. ncbi request reprint Vaccine protocols for enhanced immunogenicity of exogenous antigens
    Antje Heit
    Institute for Medical Microbiology, Immunology and Hygiene, Trogerstrasse 30, D 81675 Munchen, Germany
    Int J Med Microbiol 298:27-32. 2008
    ..These novel vaccine protocols allow the generation of robust T-cell and B-cell responses that match immunogenicity of live vectors. Their characteristics with regard to efficacy, flexibility, and clinical applicability are discussed...
  53. ncbi request reprint Cellular recognition of trimyristoylated peptide or enterobacterial lipopolysaccharide via both TLR2 and TLR4
    Stephan Spiller
    Institute of Medical Microbiology, Immunology, and Hygiene, Technical University of Munich, 81675 Munich, Germany
    J Biol Chem 282:13190-8. 2007
    ..On the other hand, simultaneous blockage of both of the two TLRs might effectively inhibit infection-induced pathology...
  54. ncbi request reprint Structural model of MD-2 and functional role of its basic amino acid clusters involved in cellular lipopolysaccharide recognition
    Anton Gruber
    Institute of Medical Microbiology, Immunology, and Hygiene, Technical University of Munich, 81675 Munich, Germany
    J Biol Chem 279:28475-82. 2004
    ..Our structural model of MD-2 is corroborated by biochemical analysis and contributes to the unraveling of molecular interactions in LPS recognition...
  55. ncbi request reprint Chlamydophila pneumoniae downregulates MHC-class II expression by two cell type-specific mechanisms
    Georg Peschel
    Institut fur Medizinische Mikrobiologie, Immunologie und Hygiene, Technische Universitat Munchen, Trogerstr 30, 81675 Munchen, Germany
    Mol Microbiol 76:648-61. 2010
    ..In summary, C. pneumoniae downregulates MHC-class II expression by two cell type-specific mechanisms which are either CPAF-independent and MyD88-dependent like in BMM or CPAF-dependent like in MEFs...
  56. doi request reprint Hemozoin: malaria's "built-in" adjuvant and TLR9 agonist
    Hermann Wagner
    Institut fur Medizinische Mikrobiologie, Immunologie und Hygiene, Technische Universitat Munchen, Trogerstr 30, 81675 Munchen, Germany
    Cell Host Microbe 7:5-6. 2010
    ....
  57. ncbi request reprint Interferon-regulatory-factor 1 controls Toll-like receptor 9-mediated IFN-beta production in myeloid dendritic cells
    Frank Schmitz
    Institute for Medical Microbiology, Immunology and Hygiene, Munich, Germany
    Eur J Immunol 37:315-27. 2007
    ..These results demonstrate that TLR-9 activation of mDC and macrophages contributes to antiviral immunity via IRF-1...
  58. pmc Selective depletion of Foxp3+ regulatory T cells induces a scurfy-like disease
    Katharina Lahl
    Institut fur Medizinische Mikrobiologie, Immunologie und Hygiene, Technische Universitat Munchen, 81675 Munich, Germany
    J Exp Med 204:57-63. 2007
    ..Furthermore, DEREG mice will allow a more precise definition of the function of Foxp3+ T reg cells in immune reactions in vivo...
  59. ncbi request reprint Alternating 2'-O-ribose methylation is a universal approach for generating non-stimulatory siRNA by acting as TLR7 antagonist
    Svetlana Hamm
    Institut fur Medizinische Mikrobiologie, Immunologie und Hygiene, Technische Universitat Munchen, Munchen, Germany
    Immunobiology 215:559-69. 2010
    ....
  60. ncbi request reprint Endogenous TLR ligands and autoimmunity
    Hermann Wagner
    Institute of Medical Microbiology, Immunology and Hygiene, 81675 Munich, Germany
    Adv Immunol 91:159-73. 2006
    ..I conclude that endogeneous ligand recognition by TLRs drives sterile inflammation sustained by innate immune cells in certain autoimmune disorders...
  61. ncbi request reprint Toll-like receptor 9-independent aggravation of glomerulonephritis in a novel model of SLE
    Philipp Yu
    Institute of Medical Microbiology, Immunology and Hygiene, Technical University of Munich, Trogerstrasse 4a, 81675 Munich, Germany
    Int Immunol 18:1211-9. 2006
    ..These results suggest that engagement of a TLR9-independent DNA activation pathway may promote autoimmunity, while TLR9 signaling can ameliorate SLE-like immune pathology in vivo...
  62. ncbi request reprint Differential involvement of TLR2 and TLR4 in host survival during pulmonary infection with Chlamydia pneumoniae
    Nuria Rodriguez
    Institute of Medical Microbiology, Immunology and Hygiene, Technical University of Munich, Munich, Germany
    Eur J Immunol 36:1145-55. 2006
    ..All TLR2(-/-) x TLR4(d/d) mice succumbed to the infection, while about 50% of TLR2(-/-) mice died. Taken together, the function of TLR2 and TLR4 is required to survive pulmonary infection with C. pneumoniae...
  63. ncbi request reprint Murine TLR2 expression analysis and systemic antagonism by usage of specific monoclonal antibodies
    Guangxun Meng
    Institute of Medical Microbiology, Immunology, and Hygiene, Technical University of Munich, Trogerstr 9, 81675 Munich, Germany
    Immunol Lett 98:200-7. 2005
    ..4 protected mice from lethal shock-like syndrome in an experimental low-dose model of septic shock. This result validates blockage of cell surface TLR2 for inhibition of immune cell over-activation upon microbial challenge...
  64. pmc Of men, mice and pigs: looking at their plasmacytoid dendritic cells [corrected]
    Hubertus Hochrein
    Institute of Medical Microbiology, Immunology and Hygiene, Technical University of Munich, Munich, Germany
    Immunology 112:26-7. 2004
  65. ncbi request reprint IL-4 regulates IL-12 p40 expression post-transcriptionally as well as via a promoter-based mechanism
    Irene Seegmüller
    Institute for Medical Microbiology, Immunology and Hygiene, Technical University of Munich, Germany
    Eur J Immunol 33:428-33. 2003
    ..These data show that IL-4 regulates IL-12 p40 expression by influencing promoter activity and by interfering with mRNA stability...
  66. pmc Compartmentalized production of CCL17 in vivo: strong inducibility in peripheral dendritic cells contrasts selective absence from the spleen
    Judith Alferink
    Institute for Medical Microbiology, Immunology and Hygiene, Technical University of Munich, D 81675 Munich, Germany
    J Exp Med 197:585-99. 2003
    ..These findings indicate that CCL17 production is a hallmark of local DC stimulation in peripheral organs but is absent from the spleen as a filter of blood-borne antigens...
  67. ncbi request reprint Generation of neutralizing mouse anti-mouse IL-18 antibodies for inhibition of inflammatory responses in vivo
    Matthias Lochner
    Institute for Medical Microbiology, Immunology and Hygiene, Technical University of Munich, Munich, Germany
    J Immunol Methods 259:149-57. 2002
    ....
  68. doi request reprint Subversion of Toll-like receptor signaling by a unique family of bacterial Toll/interleukin-1 receptor domain-containing proteins
    Christine Cirl
    Institut fur Medizinische Mikrobiologie, Immunologie und Hygiene, Technische Universitat Munchen, Trogerstrasse 30, D 81675 Munchen, Germany
    Nat Med 14:399-406. 2008
    ..Tcps represent a new class of virulence factors that act by inhibiting TLR- and MyD88-specific signaling, thus suppressing innate immunity and increasing virulence...
  69. ncbi request reprint Human and mouse plasmacytoid dendritic cells
    Hubertus Hochrein
    Institute of Medical Microbiology, Immunology and Hygiene, Technical University of Munich, Munich, Germany
    Hum Immunol 63:1103-10. 2002
    ....
  70. ncbi request reprint Lymphoid follicle destruction and immunosuppression after repeated CpG oligodeoxynucleotide administration
    Mathias Heikenwalder
    Institute of Neuropathology, University Hospital of Zurich, Schmelzbergstrasse 12, CH 8091 Zurich, Switzerland
    Nat Med 10:187-92. 2004
    ....
  71. ncbi request reprint The Toll-like receptor 7 (TLR7)-specific stimulus loxoribine uncovers a strong relationship within the TLR7, 8 and 9 subfamily
    Florian Heil
    Institute for Medical Microbiology, Hygiene and Immunology, Munich, Germany
    Eur J Immunol 33:2987-97. 2003
    ..We thus conclude that TLR7, 8 and 9 form a functional subgroup within the TLR family that recognizes pathogen-associated molecular patterns in endosomal/lysosomal compartments...
  72. ncbi request reprint Toll-like receptor 2 participates in mediation of immune response in experimental pneumococcal meningitis
    Uwe Koedel
    Department of Neurology, Klinikum Grosshadern, Ludwig Maximilians University, Munich, Germany
    J Immunol 170:438-44. 2003
    ..Thus, while TLR2 plays a significant role in vivo, yet undefined pattern recognition receptors contribute to the recognition of and initiation of the host immune defense toward Streptococcus pneumoniae infection...
  73. ncbi request reprint The major surface protein of Wolbachia endosymbionts in filarial nematodes elicits immune responses through TLR2 and TLR4
    Norbert W Brattig
    Tropical Medicine Section, Bernhard Nocht Institute for Tropical Medicine, Bernhard Nocht Strasse 74, D 20359 Hamburg, Germany
    J Immunol 173:437-45. 2004
    ..Thus, filarial helminths, through products of their endobacteria such as WSP, acquire characteristics of a typical microbial pathogen inducing immune responses via TLR2 and TLR4...
  74. ncbi request reprint A dominant role of Toll-like receptor 4 in the signaling of apoptosis in bacteria-faced macrophages
    Rudolf Haase
    Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Munich, Germany
    J Immunol 171:4294-303. 2003
    ..Together, these data show that TLR4 could act as a potent inducer of apoptosis in macrophages that encounter a bacterial pathogen...
  75. ncbi request reprint Immunostimulating capacities of stabilized RNA molecules
    Birgit Scheel
    CureVac GmbH, Tubingen, Germany
    Eur J Immunol 34:537-47. 2004
    ..We postulate that phosphorothioate RNA, which mimics trans-stabilized RNA, is a new PAMP...
  76. ncbi request reprint Immunoglobulin-like transcripts ILT2, ILT3 and ILT7 are expressed by human dendritic cells and down-regulated following activation
    Xin Sheng Ju
    Max Delbruck Center for Molecular Medicine, MDC, Robert Rossle Str 10, 13092, Berlin, Germany
    Gene 331:159-64. 2004
    ..Thus, activation of human DC with such stimuli involves down-regulation of inhibitory ILT2 and ILT3 receptors, and this could represent a novel mechanism contributing to DC activation...
  77. ncbi request reprint Specificity in Toll-like receptor signalling through distinct effector functions of TRAF3 and TRAF6
    Hans Hacker
    Laboratory of Gene Regulation and Signal Transduction, Department of Pharmacology, School of Medicine, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093, USA
    Nature 439:204-7. 2006
    ....
  78. ncbi request reprint The endoplasmic reticulum-resident heat shock protein Gp96 activates dendritic cells via the Toll-like receptor 2/4 pathway
    Ramunas M Vabulas
    Institute for Cell Biology, Department of Immunology, University of Tubingen, Auf der Morgenstelle 15, D 72076 Tubingen, Germany
    J Biol Chem 277:20847-53. 2002
    ..This feature explains the remarkable ability of Gp96 to induce specific immune responses against tumors and pathogens...
  79. ncbi request reprint Toll-like receptor-dependent activation of several human blood cell types by protamine-condensed mRNA
    Birgit Scheel
    CureVac GmbH, Tubingen, Germany
    Eur J Immunol 35:1557-66. 2005
    ..The studies presented here explain the capacity of protamine-RNA to act as a vaccine, and pave the way towards the development of safe and efficient mRNA-based immunotherapies...
  80. ncbi request reprint Activation of toll-like receptor-9 induces progression of renal disease in MRL-Fas(lpr) mice
    Hans Joachim Anders
    Nephrological Center, Medical Policlinic, Ludwig Maximilians University Munich, Germany
    FASEB J 18:534-6. 2004
    ..We conclude that TLR9 activation triggers disease activity of systemic autoimmunity, for example, lupus nephritis, and that adaptive and innate immune mechanisms contribute to the CpG-DNA-induced progression of lupus nephritis...
  81. ncbi request reprint Human TLR7 or TLR8 independently confer responsiveness to the antiviral compound R-848
    Marion Jurk
    Nat Immunol 3:499. 2002
  82. ncbi request reprint Induction of nuclear factor- kappa B and c-Jun/activator protein-1 via toll-like receptor 2 in macrophages by antimycotic-treated Candida albicans
    Alexander Roeder
    Department of Dermatology, Ludwig Maximilian University, Munich, Germany
    J Infect Dis 190:1318-26. 2004
    ..albicans demonstrated involvement of the transcription factors nuclear factor- kappa B and c-Jun/activator protein-1 and of the mitogen-activated protein kinases p38, extracellular-related kinase, and c-Jun NH(2)-terminal kinase...
  83. ncbi request reprint Toll-like receptor 9 binds single-stranded CpG-DNA in a sequence- and pH-dependent manner
    Mark Rutz
    Institute for Medical Microbiology, Hygiene and Immunology, Munich, Germany
    Eur J Immunol 34:2541-50. 2004
    ....
  84. ncbi request reprint MyD88 is required for mounting a robust host immune response to Streptococcus pneumoniae in the CNS
    Uwe Koedel
    Department of Neurology, Klinikum Grosshadern, Ludwig Maximilians University, Marchioninistrasse 15, D 81377 Munich, Germany
    Brain 127:1437-45. 2004
    ..The role played by MyD88 in mounting an immune response to Streptococcus pneumoniae, however, seems to be dependent on the anatomical compartment involved...
  85. pmc Differential contribution of Toll-like receptors 4 and 2 to the cytokine response to Salmonella enterica serovar Typhimurium and Staphylococcus aureus in mice
    Annalisa Lembo
    Max Planck Institut fur Immunbiologie, Stubeweg 51, 79108 Freiburg, Germany
    Infect Immun 71:6058-62. 2003
    ..Gram-negative bacteria induced cytokines primarily via TLR4; the contribution of TLR2 was only minor. Neither TLR4 nor, surprisingly, TLR2 was required in the MyD88-dependent response to Staphylococcus aureus...
  86. ncbi request reprint Vaccination of mice against invasive aspergillosis with recombinant Aspergillus proteins and CpG oligodeoxynucleotides as adjuvants
    Silvia Bozza
    Microbiology Section, Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Via del Giochetto, 06122 Perugia, Italy
    Microbes Infect 4:1281-90. 2002
    ..Therefore, ODNs act as a potent adjuvant for the vaccine-induced protection against the fungus by promoting dominant Th1 response to Aspergillus antigens and allergens...
  87. doi request reprint Containment of aerogenic Mycobacterium tuberculosis infection in mice does not require MyD88 adaptor function for TLR2, -4 and -9
    Christoph Holscher
    Junior Research Group Molecular Infection Biology, Research Center Borstel, Borstel, Germany
    Eur J Immunol 38:680-94. 2008
    ..Rather, MyD88, but not TLR2, TLR4 and TLR9, is critical for triggering macrophage effector mechanisms central to anti-mycobacterial defense...
  88. ncbi request reprint TLR4/MD-2 monoclonal antibody therapy affords protection in experimental models of septic shock
    Bruno Daubeuf
    NovImmune SA, Geneva, Switzerland
    J Immunol 179:6107-14. 2007
    ..These results demonstrate that neutralizing TLR4/MD-2 is highly efficacious in protecting against bacterial infection-induced toxemia and offers TLR4/MD-2 mAb treatment as a potential therapy for numerous clinical indications...
  89. ncbi request reprint IL-6 and maturation govern TLR2 and TLR4 induced TLR agonist tolerance and cross-tolerance in dendritic cells
    Julia Geisel
    Institute of Medical Microbiology and Hygiene, University of Tubingen, Tubingen, Germany
    J Immunol 179:5811-8. 2007
    ....
  90. pmc Toll-like receptor 2-deficient mice succumb to Mycobacterium tuberculosis infection
    Michael B Drennan
    Department of Immunology, Groote Schuur Hospital, University of Cape Town, Cape Town, South Africa
    Am J Pathol 164:49-57. 2004
    ..Taken together, the data suggests that TLR2 may function as a regulator of inflammation, and in its absence an exaggerated immune inflammatory response develops...
  91. ncbi request reprint Characterization and investigation of single nucleotide polymorphisms and a novel TLR2 mutation in the human TLR2 gene
    Sabine Merx
    Institute for Clinical Chemistry, Medical Faculty Mannheim, University of Heidelberg, Theodor Kutzer Ufer 1 3, D 68167 Mannheim, Germany
    Hum Mol Genet 16:1225-32. 2007
    ..Except for 10 heterozygote donors of the Arg753Gln variant determined to be prevalent in 9.4% of the tested individuals, none of the other SNPs was found in this population...
  92. ncbi request reprint Yersinia enterocolitica YopP inhibits MAP kinase-mediated antigen uptake in dendritic cells
    Stella E Autenrieth
    Institut fur Medizinische Mikrobiologie und Hygiene, Universitat Tubingen, Tubingen, Germany
    Cell Microbiol 9:425-37. 2007
    ..Together, our data demonstrate that (i) MAPK play an important role in antigen uptake by CME in DCs, and (ii) that YopP inhibits this pathway of antigen uptake in DCs, which might contribute to evasion of adaptive immunity...
  93. pmc Direct Toll-like receptor 2 mediated co-stimulation of T cells in the mouse system as a basis for chronic inflammatory joint disease
    Vera Sobek
    Department of Cellular Immunology, Max Planck Institut fur Immunbiologie, Freiburg, Germany
    Arthritis Res Ther 6:R433-46. 2004
    ....
  94. ncbi request reprint U1 small nuclear ribonucleoprotein immune complexes induce type I interferon in plasmacytoid dendritic cells through TLR7
    Emina Savarese
    Department of Internal Medicine, Technical University Munich, Germany
    Blood 107:3229-34. 2006
    ..The U1snRNA component of U1snRNP immune complexes, found in patients with SLE, acts as an endogenous "self" ligand for Tlr7 and triggers IFN-alpha and IL-6 production in PDCs...
  95. ncbi request reprint Role of immunostimulatory DNA and TLR9 in gene therapy
    Kei Yasuda
    Renal Section, Department of Medicine, Boston University School of Medicine, Boston, Massachusetts, USA
    Crit Rev Ther Drug Carrier Syst 23:89-110. 2006
    ..Moreover, recent research shows that TLR9-independent immunoactivation could take place. Here, we have attempted to present an overview of immunoactivation by DNA to optimize DNA-based therapies...