H Karch

Summary

Country: Germany

Publications

  1. ncbi request reprint Cloning and sequencing of a Shiga-like toxin II-related gene from Escherichia coli O157:H7 strain 7279
    T Meyer
    Institut für Hygiene und Mikrobiologie, Universitat, Wurzburg
    Zentralbl Bakteriol 276:176-88. 1992
  2. pmc A genomic island, termed high-pathogenicity island, is present in certain non-O157 Shiga toxin-producing Escherichia coli clonal lineages
    H Karch
    Institut für Hygiene und Mikrobiologie, D 97080 Wurzburg, 97070 Wurzburg, Germany
    Infect Immun 67:5994-6001. 1999
  3. pmc Isolation of enterohemorrhagic Escherichia coli O157 strains from patients with hemolytic-uremic syndrome by using immunomagnetic separation, DNA-based methods, and direct culture
    H Karch
    Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Germany
    J Clin Microbiol 34:516-9. 1996
  4. ncbi request reprint Analysis of the EHEC hly operon and its location in the physical map of the large plasmid of enterohaemorrhagic Escherichia coli O157:h7
    H Schmidt
    Institut für Hygiene und Mikrobiologie, Universitat Wurzburg, Germany
    Microbiology 142:907-14. 1996
  5. ncbi request reprint The large plasmids of Shiga-toxin-producing Escherichia coli (STEC) are highly variable genetic elements
    W Brunder
    Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Germany
    Microbiology 145:1005-14. 1999
  6. ncbi request reprint Non-O157:H7 pathogenic Shiga toxin-producing Escherichia coli: phenotypic and genetic profiling of virulence traits and evidence for clonality
    H Schmidt
    Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Germany
    J Infect Dis 179:115-23. 1999
  7. pmc Novel type of fimbriae encoded by the large plasmid of sorbitol-fermenting enterohemorrhagic Escherichia coli O157:H(-)
    W Brunder
    Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, D 97080 Wurzburg, Germany
    Infect Immun 69:4447-57. 2001
  8. pmc Identification and characterization of a novel genomic island integrated at selC in locus of enterocyte effacement-negative, Shiga toxin-producing Escherichia coli
    H Schmidt
    Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Wurzburg, Germany
    Infect Immun 69:6863-73. 2001
  9. ncbi request reprint KatP, a novel catalase-peroxidase encoded by the large plasmid of enterohaemorrhagic Escherichia coli O157:H7
    W Brunder
    Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Germany
    Microbiology 142:3305-15. 1996
  10. pmc Transduction of enteric Escherichia coli isolates with a derivative of Shiga toxin 2-encoding bacteriophage phi3538 isolated from Escherichia coli O157:H7
    H Schmidt
    Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, 97080 Wurzburg, Germany
    Appl Environ Microbiol 65:3855-61. 1999

Collaborators

Detail Information

Publications34

  1. ncbi request reprint Cloning and sequencing of a Shiga-like toxin II-related gene from Escherichia coli O157:H7 strain 7279
    T Meyer
    Institut für Hygiene und Mikrobiologie, Universitat, Wurzburg
    Zentralbl Bakteriol 276:176-88. 1992
    ..Immunological differences of SLT-IIvhc and SLT-II as well as their different toxicity to HeLa cells presumably resulted from the small deviations within the primary structure of the B subunit...
  2. pmc A genomic island, termed high-pathogenicity island, is present in certain non-O157 Shiga toxin-producing Escherichia coli clonal lineages
    H Karch
    Institut für Hygiene und Mikrobiologie, D 97080 Wurzburg, 97070 Wurzburg, Germany
    Infect Immun 67:5994-6001. 1999
    ..The hypothesis that the HPI in STEC contributes to the fitness of the strains in certain ecological niches rather than to their pathogenic potential is discussed...
  3. pmc Isolation of enterohemorrhagic Escherichia coli O157 strains from patients with hemolytic-uremic syndrome by using immunomagnetic separation, DNA-based methods, and direct culture
    H Karch
    Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Germany
    J Clin Microbiol 34:516-9. 1996
    ..However, because a significant number of HUS cases were attributable to non-O157 EHEC serogroups, the use of additional methods besides IMS in the bacteriological diagnosis of HUS is necessary...
  4. ncbi request reprint Analysis of the EHEC hly operon and its location in the physical map of the large plasmid of enterohaemorrhagic Escherichia coli O157:h7
    H Schmidt
    Institut für Hygiene und Mikrobiologie, Universitat Wurzburg, Germany
    Microbiology 142:907-14. 1996
    ..It was located close to the EHEC hly operon...
  5. ncbi request reprint The large plasmids of Shiga-toxin-producing Escherichia coli (STEC) are highly variable genetic elements
    W Brunder
    Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Germany
    Microbiology 145:1005-14. 1999
    ..The EHEC-hlyA gene, however, is present in both. In conclusion, this study shows that the large plasmids of STEC are not uniform genetic elements but heterogeneous in both their gene composition and arrangement...
  6. ncbi request reprint Non-O157:H7 pathogenic Shiga toxin-producing Escherichia coli: phenotypic and genetic profiling of virulence traits and evidence for clonality
    H Schmidt
    Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Germany
    J Infect Dis 179:115-23. 1999
    ..Pathogenic STEC O26, O103, and O111 belong to their own lineages and have unique profiles of virulence traits that are different from the virulence profile of E. coli O157:H7...
  7. pmc Novel type of fimbriae encoded by the large plasmid of sorbitol-fermenting enterohemorrhagic Escherichia coli O157:H(-)
    W Brunder
    Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, D 97080 Wurzburg, Germany
    Infect Immun 69:4447-57. 2001
    ..The sfp gene cluster is flanked by two blocks of insertion sequences and an origin of plasmid replication, indicating that horizontal gene transfer may have contributed to the presence of Sfp fimbriae in SF EHEC O157:H(-)...
  8. pmc Identification and characterization of a novel genomic island integrated at selC in locus of enterocyte effacement-negative, Shiga toxin-producing Escherichia coli
    H Schmidt
    Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Wurzburg, Germany
    Infect Immun 69:6863-73. 2001
    ..We conclude that the newly described island occurs exclusively in a subgroup of STEC strains that are eae negative and contain the variant stx(2d )gene...
  9. ncbi request reprint KatP, a novel catalase-peroxidase encoded by the large plasmid of enterohaemorrhagic Escherichia coli O157:H7
    W Brunder
    Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Germany
    Microbiology 142:3305-15. 1996
    ..coli) revealed a close association between the occurrence of EHEC-haemolysin and the katP gene in Shiga-like-toxin-producing E. coli O157 strains...
  10. pmc Transduction of enteric Escherichia coli isolates with a derivative of Shiga toxin 2-encoding bacteriophage phi3538 isolated from Escherichia coli O157:H7
    H Schmidt
    Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, 97080 Wurzburg, Germany
    Appl Environ Microbiol 65:3855-61. 1999
    ..coli and that the lysogens produced infectious phage progeny. Stx-encoding bacteriophages are able to spread stx genes among enteric E. coli strains...
  11. pmc Enterohemolytic phenotypes and genotypes of shiga toxin-producing Escherichia coli O111 strains from patients with diarrhea and hemolytic-uremic syndrome
    H Schmidt
    Institut für Hygiene und Mikrobiologie, Universitat Wurzburg, Germany
    J Clin Microbiol 34:2364-7. 1996
    ....
  12. ncbi request reprint EspP, a novel extracellular serine protease of enterohaemorrhagic Escherichia coli O157:H7 cleaves human coagulation factor V
    W Brunder
    Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Germany
    Mol Microbiol 24:767-78. 1997
    ..Functional analysis showed that EspP is a protease capable of cleaving pepsin A and human coagulation factor V. Degradation of factor V could contribute to the mucosal haemorrhage observed in patients with haemorrhagic colitis...
  13. ncbi request reprint Genome plasticity in Enterobacteriaceae
    W Brunder
    Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Germany
    Int J Med Microbiol 290:153-65. 2000
    ..This review deals not only with the mechanisms of genome rearrangements and the horizontal transfer of genes in Enterobacteriaceae but also points out that mobile genetic elements themselves are subjected to variation...
  14. pmc Structure and function of plasmid pColD157 of enterohemorrhagic Escherichia coli O157 and its distribution among strains from patients with diarrhea and hemolytic-uremic syndrome
    C Hofinger
    Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Germany
    J Clin Microbiol 36:24-9. 1998
    ..coli O157 strains isolated between 1987 and 1991 harbored plasmid pColD157, only 1 of 50 strains isolated during 1996 carried this plasmid. In addition, all strains harboring plasmid pColD157 were shown to have colicinogenic activity...
  15. pmc Expression of hemin receptor molecule ChuA is influenced by RfaH in uropathogenic Escherichia coli strain 536
    G Nagy
    Institut für Molekulare Infektionsbiologie, Universitat Wurzburg, 97070 Wurzburg, Germany
    Infect Immun 69:1924-8. 2001
    ..Furthermore, the presence of two different subclasses of the chuA determinant and their distribution in E. coli pathogroups are described...
  16. pmc Molecular analysis of the plasmid-encoded hemolysin of Escherichia coli O157:H7 strain EDL 933
    H Schmidt
    Institut für Hygiene und Mikrobiologie, Universität Wüzburg, Germany
    Infect Immun 63:1055-61. 1995
    ..The EHEC hemolysin appears to have clinical importance because it occurs in all O157 strains tested and is reactive to sera of patients with hemolytic uremic syndrome...
  17. ncbi request reprint A gene cluster closely related to type II secretion pathway operons of gram-negative bacteria is located on the large plasmid of enterohemorrhagic Escherichia coli O157 strains
    H Schmidt
    Institut für Hygiene und Mikrobiologie, Universitat Wurzburg, Germany
    FEMS Microbiol Lett 148:265-72. 1997
    ..coli, enteroaggregative E. coli, enterotoxigenic E. coli and enteroinvasive E. coli. The results obtained with the etp probe were confirmed by a PCR approach to specifically detect an internal fragment of the etpD gene...
  18. ncbi request reprint Antibacterials that are used as growth promoters in animal husbandry can affect the release of Shiga-toxin-2-converting bacteriophages and Shiga toxin 2 from Escherichia coli strains
    B Kohler
    Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Germany
    Microbiology 146:1085-90. 2000
    ..In vivo induction of Stx phages from lysogenic STEC may increase the amount of free phages in the intestine and therefore may contribute to the spread of STEC and development of new STEC pathotypes...
  19. ncbi request reprint Heterogeneity of the prtC gene of Porphyromonas gingivalis
    M Wittstock
    Institut fur Hygiene und Mikrobiologie der Universitat Wurzburg, Bau 17, Josef Schneider Str 2, D 97080 Wurzburg, Germany
    Oral Microbiol Immunol 15:33-9. 2000
    ..The observed heterogeneity of prtC genes may be a result of microevolution processes...
  20. pmc Cloning and expression of the dnaK gene of Campylobacter jejuni and antigenicity of heat shock protein 70
    F L Thies
    Department of Neurology, Julius Maximilians Universitat, D 97080 Wurzburg, Germany
    Infect Immun 67:1194-200. 1999
    ..Antibody responses to rCjDnaK-His were significantly elevated, compared to those of healthy individuals, in about one-third of the serum specimens obtained from C. jejuni enteritis patients...
  21. ncbi request reprint Detection and characterization of Shiga toxin-producing Escherichia coli in feral pigeons
    S Morabito
    Laboratorio di Medicina Veterinaria, , Viale Regina Elena 299, I-00161 Rome, Italy
    Vet Microbiol 82:275-83. 2001
    ..Further work is needed to clarify whether these STEC may represent a cause of avian disease or even a potential health hazard for humans...
  22. pmc A long-term study on the prevalence of shiga toxin-producing Escherichia coli (STEC) on four German cattle farms
    L Geue
    Bundesforschungsanstalt fur Viruskrankheiten der Tiere, Institut für epidemiologische Diagnostik, Wusterhausen, Germany
    Epidemiol Infect 129:173-85. 2002
    ..Most STEC O26:H11 with the complete pattern of potential virulence markers were found in clinically healthy cattle. These animals may represent a risk factor for farmers and consumers...
  23. pmc Genetic diversity of intimin genes of attaching and effacing Escherichia coli strains
    W L Zhang
    Institut für Hygiene der Westfälischen Wilhelms Universität, 48149 Munster, Germany
    J Clin Microbiol 40:4486-92. 2002
    ..In conclusion, we recommend an eae nomenclature system based on the Greek alphabet and provide an updated PCR scheme for amplification and typing of E. coli eae...
  24. ncbi request reprint Urease genes in non-O157 Shiga toxin-producing Escherichia coli: mostly silent but valuable markers for pathogenicity
    A W Friedrich
    Institut fur Hygiene, Universitatsklinikum Munster, Robert Koch Strasse 41, 48149 Munster, Germany
    Clin Microbiol Infect 12:483-6. 2006
    ..The silencing of urease expression could not be linked to a stop codon in ureD. The frequent occurrence of ure genes in eae-positive STEC isolates makes them valuable markers for virulence...
  25. pmc Clonal relatedness of Shiga-like toxin-producing Escherichia coli O101 strains of human and porcine origin
    S Franke
    Institut für Hygiene und Mikrobiology, Universitat Wurzburg, Germany
    J Clin Microbiol 33:3174-8. 1995
    ..coli O101 infection of humans. For diagnostic purposes, we recommend the use of PCR primers and DNA probes complementary to slt-IIe to correctly identify such strains and to further evaluate their role in human diseases...
  26. ncbi request reprint Cloning, sequencing and molecular analysis of the Campylobacter jejuni groESL bicistronic operon
    F L Thies
    Department of Neurology, Julius Maximilians University, Wurzburg, Germany
    Microbiology 145:89-98. 1999
    ....
  27. ncbi request reprint The ClpB protein from Campylobacter jejuni: molecular characterization of the encoding gene and antigenicity of the recombinant protein
    F L Thies
    Department of Neurology, University of Wurzburg, D 97080, Wurzburg, Germany
    Gene 230:61-7. 1999
    ..In ELISA studies, IgA levels reactive to recombinant ClpB were significantly higher in sera of patients with prior C. jejuni infections than in sera obtained from healthy control persons...
  28. ncbi request reprint Purification and characterization of a phage-encoded cytotoxin from an Escherichia coli O111 strain associated with hemolytic-uremic syndrome
    H Karch
    , Hamburg
    Zentralbl Bakteriol Mikrobiol Hyg A 270:41-51. 1988
    ..Taken together, our data established CT111 as a potent cytotoxin with significant enterotoxic and neurotoxic properties similar or identical to Shiga toxin and to Shiga-like toxin I from E. coli O26:H11 and O157:H7 strains...
  29. ncbi request reprint Serum antibody reactivity against recombinant PrtC of Porphyromonas gingivalis following periodontal therapy
    T Beikler
    Department of Periodontology, Westfalian Wilhelms University, Munster, Germany
    J Periodontal Res 38:276-81. 2003
    ..In 34 patients with chronic periodontitis, the presence of IgA, IgG, and IgG subclass serum antibodies against recombinant PrtC (rPrtC) of Porphyromonas gingivalis was assessed by immunoblot analysis 24 months after therapy...
  30. pmc The high-pathogenicity island is absent in human pathogens of Salmonella enterica subspecies I but present in isolates of subspecies III and VI
    T A Oelschlaeger
    Institut für Molekulare Infektionsbiologie, University of Wurzburg, Roentgenring 11, D 97070 Wurzburg, Germany
    J Bacteriol 185:1107-11. 2003
    ..Most high-pathogenicity island-positive isolates produced yersiniabactin under iron-limited conditions and were positive for the high-molecular-weight proteins HMWP1 and HMWP2...
  31. ncbi request reprint Plasmid-mediated formaldehyde resistance in Serratia marcescens and Escherichia coli: alterations in the cell surface
    P M Kaulfers
    , , Hamburg, Federal Republic of Germany
    Zentralbl Bakteriol Mikrobiol Hyg A 266:239-48. 1987
    ..These findings suggest that the formaldehyde resistance plasmid of Serratia marcescens confer changes in cell surface proteins and surface hydrophobicity...
  32. ncbi request reprint Human antibody response during sepsis against targets expressed by methicillin resistant Staphylococcus aureus
    U Lorenz
    Department of Surgery, The University of Würzburg, Germany
    FEMS Immunol Med Microbiol 29:145-53. 2000
    ..aureus and the other protein was identified as the phosphocarrier protein Hpr of S. aureus. The identified immunodominant proteins may serve as potential targets for the development of antibody based therapy against MRSA...
  33. ncbi request reprint Escherichia coli O157 infections and unpasteurised milk
    F Allerberger
    National Reference Laboratory for Enterohaemorrhagic Escherichia coli at the Federal Public Health Laboratory, Innsbruck, Austria
    Euro Surveill 6:147-51. 2001
    ..The isolation of indistinguishable sorbitol non-fermenting Escherichia coli O157:H from contact animals supports the association between HUS and consumption of raw goats milk, and re-emphasises the importance of pasteurising milk...
  34. ncbi request reprint Molecular and phenotypic profiling of sorbitol-fermenting Escherichia coli O157:H- human isolates from Finland
    M Eklund
    Enteric Bacteria Laboratory, Department of Bacterial and Inflammatory Diseases, National Public Health Institute KTL, Helsinki, Finland
    Clin Microbiol Infect 12:634-41. 2006
    ..coli O157:H- isolates from Finland are closely related and show a close relationship with SF E. coli O157 strains isolated in Germany. This finding suggests a clonality of SF E. coli O157:H- isolates from different geographical regions...