Stefan Weger

Summary

Affiliation: Humboldt University
Country: Germany

Publications

  1. ncbi request reprint Topors, a p53 and topoisomerase I binding protein, interacts with the adeno-associated virus (AAV-2) Rep78/68 proteins and enhances AAV-2 gene expression
    Stefan Weger
    Institut für Infektionsmedizin, Abteilung Virologie, Freie Universitat Berlin, Hindenburgdamm 27, D 12203 Berlin, Germany
    J Gen Virol 83:511-6. 2002
  2. ncbi request reprint The DNA topoisomerase I binding protein topors as a novel cellular target for SUMO-1 modification: characterization of domains necessary for subcellular localization and sumolation
    Stefan Weger
    Department of Virology, Institute of Infectious Diseases, Free University of Berlin, Hindenburgdamm 27, 12203 Berlin, Germany
    Exp Cell Res 290:13-27. 2003
  3. pmc Role of the herpes simplex virus helicase-primase complex during adeno-associated virus DNA replication
    Heiko Slanina
    Institut fur Virologie, Charite Universitatsmedizin Berlin, Campus Benjamin Franklin, Hindenburgdamm 27, 12203 Berlin, Germany
    J Virol 80:5241-50. 2006
  4. ncbi request reprint The E3 ligase Topors induces the accumulation of polysumoylated forms of DNA topoisomerase I in vitro and in vivo
    Eva Hammer
    Institute of Virology, Charite Campus Benjamin Franklin, Free University of Berlin, Hindenburgdamm 27, 12203 Berlin, Germany
    FEBS Lett 581:5418-24. 2007
  5. pmc A Regulatory Element Near the 3' End of the Adeno-Associated Virus rep Gene Inhibits Adenovirus Replication in cis by Means of p40 Promoter-Associated Short Transcripts
    Stefan Weger
    Institute of Virology, Charite University Medicine Berlin, Berlin, Germany
    J Virol 90:3981-93. 2016
  6. pmc Differential contribution of adeno-associated virus type 2 Rep protein expression and nucleic acid elements to inhibition of adenoviral replication in cis and in trans
    Stefan Weger
    Institute of Virology, Campus Benjamin Franklin, Charite University Medicine Berlin, Berlin, Germany
    J Virol 88:14126-37. 2014
  7. ncbi request reprint SUMO-1 modification regulates the protein stability of the large regulatory protein Rep78 of adeno associated virus type 2 (AAV-2)
    Stefan Weger
    Institut für Infektionsmedizin, Abteilung Virologie, Charite Campus Benjamin Franklin, Freie Universitat Berlin, 12203 Berlin, Germany
    Virology 330:284-94. 2004
  8. ncbi request reprint Topors acts as a SUMO-1 E3 ligase for p53 in vitro and in vivo
    Stefan Weger
    Institute of Infectious Diseases, Department of Virology, Charite Campus Benjamin Franklin, Free University of Berlin, Hindenburgdamm 27, 12203 Berlin, Germany
    FEBS Lett 579:5007-12. 2005
  9. ncbi request reprint Identification of a cytoplasmic interaction partner of the large regulatory proteins Rep78/Rep68 of adeno-associated virus type 2 (AAV-2)
    Stefan Weger
    Institut fur Virologie, Charite Campus Benjamin Franklin, Freie Universitat Berlin, Hindenburgdamm 27, 12203 Berlin, Germany
    Virology 362:192-206. 2007
  10. pmc DNA-binding activity of adeno-associated virus Rep is required for inverted terminal repeat-dependent complex formation with herpes simplex virus ICP8
    Martin Alex
    Institute of Virology, Campus Benjamin Franklin, Charite Medical School, Berlin, Germany
    J Virol 86:2859-63. 2012

Collaborators

Detail Information

Publications30

  1. ncbi request reprint Topors, a p53 and topoisomerase I binding protein, interacts with the adeno-associated virus (AAV-2) Rep78/68 proteins and enhances AAV-2 gene expression
    Stefan Weger
    Institut für Infektionsmedizin, Abteilung Virologie, Freie Universitat Berlin, Hindenburgdamm 27, D 12203 Berlin, Germany
    J Gen Virol 83:511-6. 2002
    ..Overexpression of Topors stimulated AAV gene expression in the absence of helper virus, suggesting a function of Topors as a transcriptional regulator...
  2. ncbi request reprint The DNA topoisomerase I binding protein topors as a novel cellular target for SUMO-1 modification: characterization of domains necessary for subcellular localization and sumolation
    Stefan Weger
    Department of Virology, Institute of Infectious Diseases, Free University of Berlin, Hindenburgdamm 27, 12203 Berlin, Germany
    Exp Cell Res 290:13-27. 2003
    ..One SUMO-1 acceptor site at lysine residue 560 could be identified within this region. However, sumolation-deficient Topors mutants showed that sumolation obviously is not required for localization to nuclear speckles...
  3. pmc Role of the herpes simplex virus helicase-primase complex during adeno-associated virus DNA replication
    Heiko Slanina
    Institut fur Virologie, Charite Universitatsmedizin Berlin, Campus Benjamin Franklin, Hindenburgdamm 27, 12203 Berlin, Germany
    J Virol 80:5241-50. 2006
    ....
  4. ncbi request reprint The E3 ligase Topors induces the accumulation of polysumoylated forms of DNA topoisomerase I in vitro and in vivo
    Eva Hammer
    Institute of Virology, Charite Campus Benjamin Franklin, Free University of Berlin, Hindenburgdamm 27, 12203 Berlin, Germany
    FEBS Lett 581:5418-24. 2007
    ..Experiments with TOP1 point mutants strongly suggest that the high-molecular weight conjugates represent SUMO-1 chains formed on a limited number of SUMO-1 acceptor sites...
  5. pmc A Regulatory Element Near the 3' End of the Adeno-Associated Virus rep Gene Inhibits Adenovirus Replication in cis by Means of p40 Promoter-Associated Short Transcripts
    Stefan Weger
    Institute of Virology, Charite University Medicine Berlin, Berlin, Germany
    J Virol 90:3981-93. 2016
    ..These findings have important implications for the future generation of Ad5/AAV hybrid vectors...
  6. pmc Differential contribution of adeno-associated virus type 2 Rep protein expression and nucleic acid elements to inhibition of adenoviral replication in cis and in trans
    Stefan Weger
    Institute of Virology, Campus Benjamin Franklin, Charite University Medicine Berlin, Berlin, Germany
    J Virol 88:14126-37. 2014
    ..Thus, very different AAV-2 elements and mechanisms are involved in inhibition of adenoviral replication during rAd/AAV-2 vector propagation and after Ad-AAV coinfection...
  7. ncbi request reprint SUMO-1 modification regulates the protein stability of the large regulatory protein Rep78 of adeno associated virus type 2 (AAV-2)
    Stefan Weger
    Institut für Infektionsmedizin, Abteilung Virologie, Charite Campus Benjamin Franklin, Freie Universitat Berlin, 12203 Berlin, Germany
    Virology 330:284-94. 2004
    ..This finding implicates a role for sumolation in the regulation of Rep78 protein stability that is assumed to be critical for the establishment and maintenance of AAV latency...
  8. ncbi request reprint Topors acts as a SUMO-1 E3 ligase for p53 in vitro and in vivo
    Stefan Weger
    Institute of Infectious Diseases, Department of Virology, Charite Campus Benjamin Franklin, Free University of Berlin, Hindenburgdamm 27, 12203 Berlin, Germany
    FEBS Lett 579:5007-12. 2005
    ..In HeLa cells, Topors induced p53 sumoylation was accompanied by an increase in endogenous p53 protein levels. Furthermore, Topors enhances the sumoylation of a variety of other, yet unidentified, cellular proteins...
  9. ncbi request reprint Identification of a cytoplasmic interaction partner of the large regulatory proteins Rep78/Rep68 of adeno-associated virus type 2 (AAV-2)
    Stefan Weger
    Institut fur Virologie, Charite Campus Benjamin Franklin, Freie Universitat Berlin, Hindenburgdamm 27, 12203 Berlin, Germany
    Virology 362:192-206. 2007
    ..Our study expands the panel of already identified nuclear Rep interaction partners to a cytoplasmic protein, which raises the awareness that important steps in the AAV life cycle may be regulated in this compartment...
  10. pmc DNA-binding activity of adeno-associated virus Rep is required for inverted terminal repeat-dependent complex formation with herpes simplex virus ICP8
    Martin Alex
    Institute of Virology, Campus Benjamin Franklin, Charite Medical School, Berlin, Germany
    J Virol 86:2859-63. 2012
    ..Our data suggest that HSV-dependent AAV replication is initiated by Rep contacting the AAV ITR and by cooperative binding of ICP8 on AAV ssDNA...
  11. doi request reprint Inhibition of adenovirus infections by siRNA-mediated silencing of early and late adenoviral gene functions
    Anne Eckstein
    Department of Cardiology and Pneumology, Charite Universitatsmedizin Berlin, Campus Benjamin Franklin, Berlin, Germany
    Antiviral Res 88:86-94. 2010
    ..Thus, an enhanced anti-adenoviral efficiency of RNAi-based inhibition strategies can be achieved by co-silencing of early and late adenoviral genes, with down regulation of the E1A as a crucial factor...
  12. pmc Roles of E4orf6 and VA I RNA in adenovirus-mediated stimulation of human parvovirus B19 DNA replication and structural gene expression
    Kerstin Winter
    Institute of Virology, Campus Benjamin Franklin, Charite University Medicine Berlin, Berlin, Germany
    J Virol 86:5099-109. 2012
    ..These data show that the cellular pathways regulating B19V replication may be very similar to those governing the productive cycle of the helper-dependent parvoviruses, the adeno-associated viruses...
  13. pmc ssDNA-dependent colocalization of adeno-associated virus Rep and herpes simplex virus ICP8 in nuclear replication domains
    Regine Heilbronn
    Institut für Infektionsmedizin, Abt Virologie, Charite Campus Benjamin Franklin, Freie Universitat Berlin, Germany
    Nucleic Acids Res 31:6206-13. 2003
    ..This novel mechanism for recruiting components of a functional replication complex directs AAV to subnuclear HSV replication compartments where the HSV replication complex can replicate the AAV genome...
  14. pmc Integration preferences of wildtype AAV-2 for consensus rep-binding sites at numerous loci in the human genome
    Daniela Hüser
    Institute of Virology, Campus Benjamin Franklin, Charite Universitatsmedizin Berlin, Berlin, Germany
    PLoS Pathog 6:e1000985. 2010
    ..In summary, targeted AAV integration is not as specific for AAVS1 as previously assumed. Rather, Rep targets AAV to integrate into open chromatin regions in the reach of various, consensus RBS homologues in the human genome...
  15. pmc Packaging of human chromosome 19-specific adeno-associated virus (AAV) integration sites in AAV virions during AAV wild-type and recombinant AAV vector production
    Daniela Hüser
    Department of Virology, Institute of Infectious Diseases, Free University of Berlin, 12203 Berlin, Germany
    J Virol 77:4881-7. 2003
    ..Apparently, AAV latency established by site-specific integration and the helper virus-dependent, productive AAV cycle are more closely related than previously thought...
  16. pmc Comprehensive Small RNA-Seq of Adeno-Associated Virus (AAV)-Infected Human Cells Detects Patterns of Novel, Non-Coding AAV RNAs in the Absence of Cellular miRNA Regulation
    Catrin Stutika
    Charite Medical School, Campus Benjamin Franklin, Institute of Virology, Berlin, Germany
    PLoS ONE 11:e0161454. 2016
    ..In contrast to other virus groups AAV infection had virtually no effect on the expression of cellular miRNA, which underscores the long-established concept that wild-type AAV infection is apathogenic. ..
  17. pmc High Prevalence of Infectious Adeno-associated Virus (AAV) in Human Peripheral Blood Mononuclear Cells Indicative of T Lymphocytes as Sites of AAV Persistence
    Daniela Hüser
    Institute of Virology, Campus Benjamin Franklin, Charite Medical School, Berlin, Germany
    J Virol 91:. 2017
    ..Immunosuppression obviously enhances AAV replication in parallel with activation of human cytomegalovirus (HCMV) and human herpesvirus 6 (HHV-6), reminiscent of herpesvirus-induced AAV activation...
  18. pmc Antibody-mediated enhancement of parvovirus B19 uptake into endothelial cells mediated by a receptor for complement factor C1q
    Kristina von Kietzell
    Institute of Virology, Campus Benjamin Franklin, Charite University Medicine Berlin, Berlin, Germany
    J Virol 88:8102-15. 2014
    ....
  19. doi request reprint Transactivation of human parvovirus B19 gene expression in endothelial cells by adenoviral helper functions
    Tanja Pozzuto
    Department of Cardiology and Pneumology, Campus Benjamin Franklin, Charite University Medicine Berlin, Hindenburgdamm 30, 12200 Berlin, Germany
    Virology 411:50-64. 2011
    ..Thus the almost complete block in B19V gene expression seen in endothelial cells can be abrogated by infection with other viruses...
  20. pmc A Comprehensive RNA Sequencing Analysis of the Adeno-Associated Virus (AAV) Type 2 Transcriptome Reveals Novel AAV Transcripts, Splice Variants, and Derived Proteins
    Catrin Stutika
    Institute of Virology, Campus Benjamin Franklin, Charite Medical School, Berlin, Germany
    J Virol 90:1278-89. 2015
    ..Upon infection with AAV alone, low-level transcription of both AAV strands is detectable and is strongly stimulated upon coinfection with Ad...
  21. pmc Long-term cardiac-targeted RNA interference for the treatment of heart failure restores cardiac function and reduces pathological hypertrophy
    Lennart Suckau
    Department of Cardiology and Pneumology, Charite University Medicine Berlin, Germany
    Circulation 119:1241-52. 2009
    ..Whereas gene therapy rests on recombinant protein expression as its basic principle, RNAi therapy uses regulatory RNAs to achieve its effect...
  22. pmc Kinetics and frequency of adeno-associated virus site-specific integration into human chromosome 19 monitored by quantitative real-time PCR
    Daniela Hüser
    Department of Virology, Institute of Infectious Diseases, Free University of Berlin, Berlin, Germany
    J Virol 76:7554-9. 2002
    ..Use of this quantitative real-time PCR will greatly facilitate the study of the early steps of wild-type and recombinant AAV vector integration...
  23. ncbi request reprint A bidirectional Tet-dependent promotor construct regulating the expression of E1A for tight control of oncolytic adenovirus replication
    Henry Fechner
    Department of Cardiology and Pulmology, Charite Universitatsmedizin Berlin, Campus Benjamin Franklin, Hindenburgdamm 30, 12200 Berlin, Germany
    J Biotechnol 127:560-74. 2007
    ..Future improvement of the Dox-dependent E1A regulation cassette should lead to the generation of an oAdV well suited to meet the demands for a highly regulated and efficient oncolytic virus for in vivo applications...
  24. doi request reprint Definition of herpes simplex virus helper functions for the replication of adeno-associated virus type 5
    Catrin Stutika
    Institute of Virology, Campus Benjamin Franklin, Charite Universitatsmedizin Berlin, Germany
    J Gen Virol 96:840-50. 2015
    ..Taken together, our findings show that productive AAV5 replication is promoted by the same combination of HSV helper functions as AAV2. ..
  25. pmc OneBac 2.0: Sf9 Cell Lines for Production of AAV5 Vectors with Enhanced Infectivity and Minimal Encapsidation of Foreign DNA
    Mario Mietzsch
    1 Institute of Virology, Campus Benjamin Franklin, Charite Medical School, Berlin, Germany
    Hum Gene Ther 26:688-97. 2015
    ....
  26. doi request reprint Interactions of cullin3/KCTD5 complexes with both cytoplasmic and nuclear proteins: Evidence for a role in protein stabilization
    Natalja Rutz
    Institute of Virology, Campus Benjamin Franklin, Charite University Medicine Berlin, Berlin, Germany
    Biochem Biophys Res Commun 464:922-8. 2015
    ..On the contrary, KCTD5 or Cullin3 overexpression increased ZNF711 protein stability. ..
  27. pmc OneBac: platform for scalable and high-titer production of adeno-associated virus serotype 1-12 vectors for gene therapy
    Mario Mietzsch
    1 Institute of Virology, Campus Benjamin Franklin, Charite Universitatsmedizin Berlin, 12203 Berlin, Germany
    Hum Gene Ther 25:212-22. 2014
    ..Thus, OneBac combines full AAV serotype options with the capacity for stable scale-up production, the current bottleneck for the transition of AAV from gene therapy trials to routine clinical treatment...
  28. doi request reprint Viral vectors for gene transfer: current status of gene therapeutics
    Regine Heilbronn
    Institute of Virology, Charite Universitatsmedizin Berlin, Hindenburgdamm 27, 12203 Berlin, Germany
    Handb Exp Pharmacol . 2010
    ..The focus is on production methods suitable for biopharmaceutical upscaling and for downstream processing. Quality control measures and biological safety considerations for the use of vectors in clinical trials are discussed...
  29. ncbi request reprint SUMOylation modulates transcriptional repression by TRPS1
    Frank J Kaiser
    Institut fur Humangenetik, Universitatsklinikum Essen, D 45122 Essen, Germany
    Biol Chem 388:381-90. 2007
    ..These results identify SUMOylation at the TRPS1-RD as a major mechanism that regulates the function of TRPS1...
  30. ncbi request reprint An improved Tet-On regulatable FasL-adenovirus vector system for lung cancer therapy
    Isaac Sipo
    Department of Cardiology and Pneumology, Charite Universitatsmedizin Berlin, Campus Benjamin Franklin, Hindenburgdamm 30, 12200 Berlin Germany
    J Mol Med (Berl) 84:215-25. 2006
    ..These data show that the novel TRE-Tight1 promoter has a high potential for closely controlled and efficient expression of cytotoxic genes in AdV-based anti-cancer approaches...