Jochen Ringe

Summary

Affiliation: Humboldt University
Country: Germany

Publications

  1. pmc A reliable protocol for the isolation of viable, chondrogenically differentiated human mesenchymal stem cells from high-density pellet cultures
    Mujib Ullah
    Tissue Engineering Laboratory and Berlin Brandenburg Center for Regenerative Therapies, Department of Rheumatology and Clinical Immunology, Charite Universitatsmedizin Berlin, Berlin, Germany
    Biores Open Access 1:297-305. 2012
  2. pmc Crosstalks between integrin alpha 5 and IGF2/IGFBP2 signalling trigger human bone marrow-derived mesenchymal stromal osteogenic differentiation
    Zahia Hamidouche
    Laboratory of Osteoblast Biology and Pathology, INSERM U606, Paris F 75475, France
    BMC Cell Biol 11:44. 2010
  3. ncbi request reprint Future of tissue engineering in rheumatic diseases
    Jochen Ringe
    Charite University Medicine Berlin, Tissue Engineering Laboratory and Berlin Brandenburg Center for Regenerative Therapies, Department of Rheumatology and Clinical Immunology, Tucholskystr 2, 10117 Berlin, Germany
    Expert Opin Biol Ther 7:283-7. 2007
  4. pmc Tissue engineering in the rheumatic diseases
    Jochen Ringe
    Tissue Engineering Laboratory and Berlin Brandenburg Center for Regenerative Therapies, Department of Rheumatology and Clinical Immunology, Charite Universitatsmedizin Berlin, Tucholskystr 2, 10117 Berlin, Germany
    Arthritis Res Ther 11:211. 2009
  5. ncbi request reprint [Mesenchymal stem cells for tissue engineering of bone and cartilage]
    Jochen Ringe
    Labor für Tissue Engineering, Medizinische Klinik mit Schwerpunkt für Rheumatologie und Klinische Immunologie, Universitatsklinikum Charite, Humboldt Uni versität Berlin
    Med Klin (Munich) 98:35-40. 2003
  6. ncbi request reprint Towards in situ tissue repair: human mesenchymal stem cells express chemokine receptors CXCR1, CXCR2 and CCR2, and migrate upon stimulation with CXCL8 but not CCL2
    Jochen Ringe
    Tissue Engineering Laboratory, Department of Rheumatology, Charite University Medicine Berlin, Berlin, Germany
    J Cell Biochem 101:135-46. 2007
  7. ncbi request reprint Stem cells for regenerative medicine: advances in the engineering of tissues and organs
    Jochen Ringe
    Department of Rheumatology, Medical Faculty Charité, Humboldt University of Berlin, Germany
    Naturwissenschaften 89:338-51. 2002
  8. ncbi request reprint Porcine mesenchymal stem cells. Induction of distinct mesenchymal cell lineages
    Jochen Ringe
    Department of Rheumatology, University Medical Center, Charite, The Humboldt University of Berlin, Germany
    Cell Tissue Res 307:321-7. 2002
  9. doi request reprint Human mastoid periosteum-derived stem cells: promising candidates for skeletal tissue engineering
    J Ringe
    Tissue Engineering Laboratory and Berlin Brandenburg Center for Regenerative Therapies, Department of Rheumatology and Clinical Immunology, Charite University Medicine Berlin, Berlin, Germany
    J Tissue Eng Regen Med 2:136-46. 2008
  10. doi request reprint Gene expression profiling of primary human articular chondrocytes in high-density micromasses reveals patterns of recovery, maintenance, re- and dedifferentiation
    Tilo Dehne
    Tissue Engineering Laboratory and Berlin Brandenburg Center for Regenerative Therapies, Department of Rheumatology and Clinical Immunology, Charite Universitatsmedizin Berlin, Tucholskystrasse 2, 10117 Berlin, Germany
    Gene 462:8-17. 2010

Collaborators

Detail Information

Publications64

  1. pmc A reliable protocol for the isolation of viable, chondrogenically differentiated human mesenchymal stem cells from high-density pellet cultures
    Mujib Ullah
    Tissue Engineering Laboratory and Berlin Brandenburg Center for Regenerative Therapies, Department of Rheumatology and Clinical Immunology, Charite Universitatsmedizin Berlin, Berlin, Germany
    Biores Open Access 1:297-305. 2012
    ..Such cells, as well as the ECM components, are of interest as research tools and for cartilage tissue engineering...
  2. pmc Crosstalks between integrin alpha 5 and IGF2/IGFBP2 signalling trigger human bone marrow-derived mesenchymal stromal osteogenic differentiation
    Zahia Hamidouche
    Laboratory of Osteoblast Biology and Pathology, INSERM U606, Paris F 75475, France
    BMC Cell Biol 11:44. 2010
    ..In this study, we used microarray analysis to identify signalling molecules that promote osteogenic differentiation in human bone marrow stroma derived MSCs...
  3. ncbi request reprint Future of tissue engineering in rheumatic diseases
    Jochen Ringe
    Charite University Medicine Berlin, Tissue Engineering Laboratory and Berlin Brandenburg Center for Regenerative Therapies, Department of Rheumatology and Clinical Immunology, Tucholskystr 2, 10117 Berlin, Germany
    Expert Opin Biol Ther 7:283-7. 2007
    ..This review focuses on state-of-the-art and future in situ mesenchymal stem cell-based tissue engineering approaches for joint repair in patients with rheumatic diseases...
  4. pmc Tissue engineering in the rheumatic diseases
    Jochen Ringe
    Tissue Engineering Laboratory and Berlin Brandenburg Center for Regenerative Therapies, Department of Rheumatology and Clinical Immunology, Charite Universitatsmedizin Berlin, Tucholskystr 2, 10117 Berlin, Germany
    Arthritis Res Ther 11:211. 2009
    ..Tissue engineering also provides highly organized three-dimensional in vitro culture models of human cells and their extracellular matrix for arthritis research...
  5. ncbi request reprint [Mesenchymal stem cells for tissue engineering of bone and cartilage]
    Jochen Ringe
    Labor für Tissue Engineering, Medizinische Klinik mit Schwerpunkt für Rheumatologie und Klinische Immunologie, Universitatsklinikum Charite, Humboldt Uni versität Berlin
    Med Klin (Munich) 98:35-40. 2003
    ..In this article, important aspects of tissue engineering of bone and cartilage will be reviewed with emphasis on mesenchymal stem cells...
  6. ncbi request reprint Towards in situ tissue repair: human mesenchymal stem cells express chemokine receptors CXCR1, CXCR2 and CCR2, and migrate upon stimulation with CXCL8 but not CCL2
    Jochen Ringe
    Tissue Engineering Laboratory, Department of Rheumatology, Charite University Medicine Berlin, Berlin, Germany
    J Cell Biochem 101:135-46. 2007
    ..In combination with already known data on MSC recruitment and differentiation these are promising results towards in situ regenerative medicine approaches based on guiding of MSC to sites of degenerated tissues...
  7. ncbi request reprint Stem cells for regenerative medicine: advances in the engineering of tissues and organs
    Jochen Ringe
    Department of Rheumatology, Medical Faculty Charité, Humboldt University of Berlin, Germany
    Naturwissenschaften 89:338-51. 2002
    ..Furthermore, several issues of importance, especially with regard to the clinical application of mesenchymal stem cells, are discussed...
  8. ncbi request reprint Porcine mesenchymal stem cells. Induction of distinct mesenchymal cell lineages
    Jochen Ringe
    Department of Rheumatology, University Medical Center, Charite, The Humboldt University of Berlin, Germany
    Cell Tissue Res 307:321-7. 2002
    ..Therefore, porcine MSC may be introduced as a valuable model system with which to study the mesenchymal lineages for basic research and tissue engineering...
  9. doi request reprint Human mastoid periosteum-derived stem cells: promising candidates for skeletal tissue engineering
    J Ringe
    Tissue Engineering Laboratory and Berlin Brandenburg Center for Regenerative Therapies, Department of Rheumatology and Clinical Immunology, Charite University Medicine Berlin, Berlin, Germany
    J Tissue Eng Regen Med 2:136-46. 2008
    ..These results suggest MSC-like PCs from mastoid as candidates for therapy of complex skeletal defects...
  10. doi request reprint Gene expression profiling of primary human articular chondrocytes in high-density micromasses reveals patterns of recovery, maintenance, re- and dedifferentiation
    Tilo Dehne
    Tissue Engineering Laboratory and Berlin Brandenburg Center for Regenerative Therapies, Department of Rheumatology and Clinical Immunology, Charite Universitatsmedizin Berlin, Tucholskystrasse 2, 10117 Berlin, Germany
    Gene 462:8-17. 2010
    ..Cultivation longer than 6 weeks leads to progressing dedifferentiation of chondrocytes that should be considered on long-term evaluations...
  11. pmc Antirheumatic drug response signatures in human chondrocytes: potential molecular targets to stimulate cartilage regeneration
    Kristin Andreas
    Tissue Engineering Laboratory and Berlin Brandenburg Center for Regenerative Therapies, Department of Rheumatology, Charite Universitatsmedizin Berlin, Tucholskystrasse 2, 10117 Berlin, Germany
    Arthritis Res Ther 11:R15. 2009
    ..This study aimed to disclose major mechanisms of antirheumatic drug action on human chondrocytes and to reveal marker and pharmacological target genes that are involved in cartilage dysfunction and regeneration...
  12. doi request reprint Human periosteum-derived progenitor cells express distinct chemokine receptors and migrate upon stimulation with CCL2, CCL25, CXCL8, CXCL12, and CXCL13
    Stefan Stich
    Tissue Engineering Laboratory and Berlin Brandenburg Center for Regenerative Therapies, Department of Rheumatology and Clinical Immunology, Charite University Medicine Berlin, Berlin, Germany
    Eur J Cell Biol 87:365-76. 2008
    ..These results are promising towards in situ bone repair therapies based on guiding PCs to bone defects, and encourage further in vivo studies...
  13. ncbi request reprint BMP7 promotes adipogenic but not osteo-/chondrogenic differentiation of adult human bone marrow-derived stem cells in high-density micro-mass culture
    Katja Neumann
    TransTissue Technologies GmbH, Tucholskystrasse 2, 10117 Berlin, Germany
    J Cell Biochem 102:626-37. 2007
    ..These results suggest that BMP7 promotes the adipogenic and not the osteogenic or chondrogenic lineage development of human stem cells when assembled three-dimensionally in micro-masses...
  14. doi request reprint Migration potential and gene expression profile of human mesenchymal stem cells induced by CCL25
    Tabea Binger
    Tissue Engineering Laboratory and Berlin Brandenburg Center for Regenerative Therapies, Department of Rheumatology and Clinical Immunology, Charite Universitatsmedizin Berlin, CCM, Tucholskystr 2, 10117 Berlin, Germany
    Exp Cell Res 315:1468-79. 2009
    ..These results suggest CCL25 as a new potential candidate for further in situ regeneration approaches...
  15. ncbi request reprint BMP2 initiates chondrogenic lineage development of adult human mesenchymal stem cells in high-density culture
    Bernhard Schmitt
    Tissue Engineering Laboratory, Department of Rheumatology, Medical Faculty Charité, Humboldt University of Berlin, Tucholskystrasse 2, 10117 Berlin, Germany
    Differentiation 71:567-77. 2003
    ..These results suggest BMP2 as a promising candidate for tissue engineering approaches regenerating articular cartilage on the basis of mesenchymal progenitors from bone marrow...
  16. pmc A method to screen and evaluate tissue adhesives for joint repair applications
    Tilo Dehne
    Tissue Engineering Laboratory and Berlin Brandenburg Center for Regenerative Therapies, Department of Rheumatology and Clinical Immunology, Charite Universitatsmedizin Berlin, Föhrer Strasse 15, Berlin 13353, Germany
    BMC Musculoskelet Disord 13:175. 2012
    ..This study was conducted to develop an easy to use method to screen and evaluate tissue adhesives for tissue engineering applications...
  17. pmc Chondrogenic differentiation potential of osteoarthritic chondrocytes and their possible use in matrix-associated autologous chondrocyte transplantation
    Tilo Dehne
    Tissue Engineering Laboratory and Berlin Brandenburg Center for Regenerative Therapies, Department of Rheumatology and Clinical Immunology, Charite Universitatsmedizin Berlin, Tucholskystrasse 2, Berlin, 10117, Germany
    Arthritis Res Ther 11:R133. 2009
    ....
  18. doi request reprint Gene expression profiling of human mesenchymal stem cells chemotactically induced with CXCL12
    Stefan Stich
    Tissue Engineering Laboratory and Berlin Brandenburg Center for Regenerative Therapies, Department of Rheumatology and Clinical Immunology, Charite University Medicine Berlin, Berlin, Germany
    Cell Tissue Res 336:225-36. 2009
    ..The obtained data provide further insights into the molecular mechanisms involved in chemotactic processes in cell migration and designate CXCL12 as a promising candidate for in situ recruitment in regenerative therapies...
  19. doi request reprint Chondrogenic differentiation capacity of human mesenchymal progenitor cells derived from subchondral cortico-spongious bone
    Katja Neumann
    TransTissue Technologies GmbH, Tucholskystrasse 2, 10117 Berlin, Germany
    J Orthop Res 26:1449-56. 2008
    ..CSP cells from subchondral bone, as known from microfracture, are multipotent stem cell-like mesenchymal progenitors with a high chondrogenic differentiation potential...
  20. doi request reprint Chemokine profile of human serum from whole blood: migratory effects of CXCL-10 and CXCL-11 on human mesenchymal stem cells
    Gregor Kalwitz
    TransTissue Technologies GmbH and Tissue Engineering Laboratory, Department of Rheumatology, Charite Universitatsmedizin Berlin, Berlin, Germany
    Connect Tissue Res 51:113-22. 2010
    ..Therefore, human serum derived from whole blood contains chemokines that may contribute to serum-mediated recruitment of human mesenchymal progenitors from bone marrow...
  21. doi request reprint Chondrogenic differentiation of human mesenchymal stem cells in micro-masses is impaired by high doses of the chemokine CXCL7
    Gregor Kalwitz
    TransTissue Technologies GmbH, Tucholskystrasse 2, 10117 Berlin, Germany
    J Tissue Eng Regen Med 5:50-9. 2011
    ..These results suggest that low doses of CXCL7 do not impair the chondrogenic differentiation of bone marrow-derived stem cells and may suited for in situ cartilage tissue engineering...
  22. pmc Reverse differentiation as a gene filtering tool in genome expression profiling of adipogenesis for fat marker gene selection and their analysis
    Mujib Ullah
    Tissue Engineering Laboratory and Berlin Brandenburg Center for Regenerative Therapies, Department of Rheumatology and Clinical Immunology, Charite University Medicine Berlin, Berlin, Germany
    PLoS ONE 8:e69754. 2013
    ..We hypothesize that exclusively adipogenic-specific genes change their expression during adipogenesis, and reverse during dedifferentiation. Thus, MSC were adipogenic differentiated and dedifferentiated...
  23. doi request reprint Transdifferentiation of mesenchymal stem cells-derived adipogenic-differentiated cells into osteogenic- or chondrogenic-differentiated cells proceeds via dedifferentiation and have a correlation with cell cycle arresting and driving genes
    Mujib Ullah
    Tissue Engineering Laboratory and Berlin Brandenburg Center for Regenerative Therapies, Dept of Rheumatology and Clinical Immunology, Charite University Medicine Berlin, Chariteplatz 1, 10117 Berlin, Germany
    Differentiation 85:78-90. 2013
    ..Regarding clinical use, the knowledge of potency and underlying mechanisms are prerequisites...
  24. pmc Key regulatory molecules of cartilage destruction in rheumatoid arthritis: an in vitro study
    Kristin Andreas
    Tissue Engineering Laboratory and Berlin Brandenburg Center for Regenerative Therapies, Department of Rheumatology, Charite Universitatsmedizin Berlin, Tucholskystrasse 2, 10117 Berlin, Germany
    Arthritis Res Ther 10:R9. 2008
    ..Hence, the objective of this study was to establish a standardized in vitro model to profile the key regulatory molecules of RA-related destruction of cartilage that are expressed by human chondrocytes...
  25. doi request reprint Extracellular matrix of adipogenically differentiated mesenchymal stem cells reveals a network of collagen filaments, mostly interwoven by hexagonal structural units
    Mujib Ullah
    Tissue Engineering Laboratory and Berlin Brandenburg Center for Regenerative Therapies, Dept of Rheumatology and Clinical Immunology, Charite University Medicine Berlin, Chariteplatz 1, 10117 Berlin, Germany Electronic address
    Matrix Biol 32:452-65. 2013
    ..Knowledge of the interactive-language between cells and matrix could be beneficial for the artificial designing of biomaterials and bioscaffolds. ..
  26. doi request reprint Immune attributes of cardiac-derived adherent proliferating (CAP) cells in cardiac therapy
    Marion Haag
    Berlin Brandenburg Centre for Regenerative Therapies, Charite Universitatsmedizin Berlin, Germany Tissue Engineering Laboratory, Charite Universitatsmedizin Berlin, Germany
    J Tissue Eng Regen Med 7:362-70. 2013
    ..The results indicate that CAP cells have low immunogenicity and could be advantageous in cardiac repair by reducing inflammatory cytokines and inducing regulatory T cells...
  27. pmc Differential gene expression profiling of human bone marrow-derived mesenchymal stem cells during adipogenic development
    Adriane Menssen
    Tissue Engineering Laboratory, Clinic for Rheumatology and Clinical Immunology, Charite Universitatsmedizin Berlin, Charite Platz 1, 10117 Berlin, Germany
    BMC Genomics 12:461. 2011
    ..For data processing and selection of adipogenic candidate genes, we used the online database SiPaGene for Affymetrix microarray expression data...
  28. doi request reprint Tissue-engineered cartilage of porcine and human origin as in vitro test system in arthritis research
    Axel R Göhring
    Tissue Engineering Laboratory and Berlin Brandenburg Center for Regenerative Therapies, Dept of Rheumatology and Clinical Immunology, Charite Universitatsmedizin Berlin, Tucholskystrasse 2, Berlin, Germany
    Biotechnol Prog 26:1116-25. 2010
    ..Therefore, we demonstrate that engineered cartilage of porcine and human origin represents a powerful in vitro model for cartilage in vivo...
  29. ncbi request reprint Tracheal remodeling: comparison of different composite cultures consisting of human respiratory epithelial cells and human chondrocytes
    Cosima Pfenninger
    Tissue Engineering Laboratory, Department of Rheumatology, Charite University Medicine Berlin, Campus Mitte, Tucholskystrasse 2, 10117 Berlin, Germany
    In Vitro Cell Dev Biol Anim 43:28-36. 2007
    ....
  30. ncbi request reprint Synovial fluid recruits human mesenchymal progenitors from subchondral spongious bone marrow
    Michaela Endres
    TransTissue Technologies GmbH, Tucholskystrasse 2, 10117 Berlin, Germany
    J Orthop Res 25:1299-307. 2007
    ..0001). The chemotactic activity of SF obtained from normal donors and from patients with degenerative joint diseases suggests that SF may be actively involved in the migration of progenitors in cartilage defects after microfracture...
  31. ncbi request reprint Gene expression profiling of rheumatoid arthritis synovial cells treated with antirheumatic drugs
    Thomas Häupl
    Tissue Engineering Laboratory, Department of Rheumatology, Charite Universitatsmedizin Berlin, Campus Mitte, Tucholskystrasse 2, 10117 Berlin, Germany
    J Biomol Screen 12:328-40. 2007
    ..Synovial fibroblasts reflect the disease-related pathophysiology and are useful tools for screening putative antirheumatic compounds...
  32. doi request reprint Gene expression profile of adult human bone marrow-derived mesenchymal stem cells stimulated by the chemokine CXCL7
    Gregor Kalwitz
    TransTissue Technologies GmbH, Tucholskystrasse 2, 10117 Berlin, Germany
    Int J Biochem Cell Biol 41:649-58. 2009
    ..Pathway analysis suggests that recruitment of human MSC by CXCL7 is supported by the induction of ligands of the interleukin 8 receptors, synergistically activating the respective signaling pathways...
  33. ncbi request reprint Gene expression profiling of human articular cartilage grafts generated by tissue engineering
    Christian Kaps
    Tissue Engineering Laboratory, Department of Rheumatology, Charite Universitatsmedizin Berlin, Campus Mitte, Tucholskystr 2, 10117 Berlin, Germany
    Biomaterials 27:3617-30. 2006
    ..Three-dimensional tissue culture of expanded articular chondrocytes initiates chondrocyte re-differentiation in vitro and leads to the maturation of cartilage grafts towards hyaline cartilage in vivo...
  34. pmc N-glycosylation profile of undifferentiated and adipogenically differentiated human bone marrow mesenchymal stem cells: towards a next generation of stem cell markers
    Houda Hamouda
    1 Institute of Laboratory Medicine, Clinical Chemistry and Pathobiochemistry, Charite Universitatsmedizin Berlin, Berlin, Germany
    Stem Cells Dev 22:3100-13. 2013
    ..These glycan structures are promising candidate markers to detect and distinguish MSCs and their adipogenic progeny. ..
  35. doi request reprint Highly efficient magnetic stem cell labeling with citrate-coated superparamagnetic iron oxide nanoparticles for MRI tracking
    Kristin Andreas
    Tissue Engineering Laboratory and Berlin Brandenburg Center for Regenerative Therapies, Department of Rheumatology and Clinical Immunology, Charite Universitatsmedizin Berlin, Chariteplatz 1, 10117 Berlin, Germany
    Biomaterials 33:4515-25. 2012
    ..Though a careful titration of SPION incorporation, cellular function and MRI visualization is essential, Citrate SPIONs are very efficient intracellular magnetic labels for in vivo stem cell tracking by MRI...
  36. doi request reprint Endomyocardial biopsy derived adherent proliferating cells - a potential cell source for cardiac tissue engineering
    Marion Haag
    Tissue Engineering Laboratory, Department of Rheumatology and Clinical Immunology, Charite Universitatsmedizin Berlin, Tucholskystr 2, 10117 Berlin, Germany
    J Cell Biochem 109:564-75. 2010
    ..Results indicate that these cells rather support angiogenesis than cardiomyocyte differentiation...
  37. pmc Human Endomyocardial Biopsy Specimen-Derived Stromal Cells Modulate Angiotensin II-Induced Cardiac Remodeling
    Kapka Miteva
    Berlin Brandenburg Center for Regenerative Therapies, Charite, University Medicine Berlin, Campus Virchow, Berlin, Germany
    Stem Cells Transl Med 5:1707-1718. 2016
    ..We conclude that CardAPs improve Ang II-induced cardiac remodeling involving antifibrotic and antihypertrophic effects via paracrine actions and immunomodulatory properties...
  38. doi request reprint A P19 and P19CL6 cell-based complementary approach to determine paracrine effects in cardiac tissue engineering
    Tilo Dehne
    Berlin Brandenburg Center for Regenerative Therapies, Charite Universitatsmedizin Berlin, Berlin, Germany
    Cells Tissues Organs 199:24-36. 2014
    ..In a complementary approach, they could be beneficial for determining the stimulatory cardiomyogenic potential of candidate cardiac-repair cells in vitro...
  39. pmc Mesenchymal stromal cells but not cardiac fibroblasts exert beneficial systemic immunomodulatory effects in experimental myocarditis
    Konstantinos Savvatis
    Department of Cardiology and Pneumology, Campus Benjamin Franklin CBF, Charite Universitatsmedizin Berlin, Berlin, Germany
    PLoS ONE 7:e41047. 2012
    ..In conclusion, modulation of the immune response in extracardiac organs is associated with cardiobeneficial effects in experimental inflammatory cardiomyopathy after systemic application of MSCs...
  40. doi request reprint Regenerative medicine in rheumatic disease-progress in tissue engineering
    Jochen Ringe
    Tissue Engineering Laboratory and Berlin Brandenburg Center for Regenerative Therapies, Department of Rheumatology and Clinical Immunology, Charite Universitatsmedizin Berlin, Chariteplatz 1, 10117 Berlin, Germany
    Nat Rev Rheumatol 8:493-8. 2012
    ..Tissue engineering faces a crucial phase to translate products into clinical routine and the regulatory framework for cell-based products in particular is an important issue...
  41. pmc Human cardiac-derived adherent proliferating cells reduce murine acute Coxsackievirus B3-induced myocarditis
    Kapka Miteva
    Berlin Brandenburg Center for Regenerative Therapies, Charite, University Medicine Berlin, Campus Virchow, Berlin, Germany
    PLoS ONE 6:e28513. 2011
    ..We explored whether CAPs application could be a novel strategy to improve acute Coxsackievirus B3 (CVB3)-induced myocarditis...
  42. doi request reprint In vivo effect of bone marrow-derived mesenchymal stem cells in a rat kidney transplantation model with prolonged cold ischemia
    Yoshiaki Hara
    Institute of Medical Immunology, Charite Universitatsmedizin Berlin, Campus Virchow Klinikum, Berlin, Germany
    Transpl Int 24:1112-23. 2011
    ..In addition, fewer antigen-presenting cells were recruited into the renal allograft. In conclusion, rat MSCs ameliorate inflammation induced by prolonged cold ischemia in kidney transplantation...
  43. ncbi request reprint Changes in the gene expression pattern of cytokeratins in human respiratory epithelial cells during culture
    Michaela Endres
    Tissue Engineering Laboratory, University Medical Center Charite, Tucholskystr 2, 10117 Berlin, Germany
    Eur Arch Otorhinolaryngol 262:390-6. 2005
    ..Our results suggest that culture-expanded hREC have the potential to colonize collagen-coated biomaterials and to regenerate epithelial cell types for tracheal tissue engineering...
  44. doi request reprint Biodegradable insulin-loaded PLGA microspheres fabricated by three different emulsification techniques: investigation for cartilage tissue engineering
    Kristin Andreas
    Berlin Brandenburg Center for Regenerative Therapies, Department of Rheumatology and Clinical Immunology, Charite Universitatsmedizin Berlin, Berlin, Germany
    Acta Biomater 7:1485-95. 2011
    ..Our results should encourage further studies applying protein-loaded PLGA microspheres in combination with cell transplants or cell-free in situ tissue engineering implants to regenerate cartilage...
  45. ncbi request reprint Growth characterization of neo porcine cartilage pellets and their use in an interactive culture model
    Carsten Lübke
    VasoTissue Technologies GmbH, Department of Research and Development, Tucholskystrasse 2, 10117 Berlin, Germany
    Osteoarthritis Cartilage 13:478-87. 2005
    ....
  46. pmc Regenerative and immunogenic characteristics of cultured nucleus pulposus cells from human cervical intervertebral discs
    Stefan Stich
    Tissue Engineering Laboratory and Berlin Brandenburg Center for Regenerative Therapies, Department of Rheumatology and Clinical Immunology, Charite Universitatsmedizin Berlin, Berlin, Germany
    PLoS ONE 10:e0126954. 2015
    ....
  47. doi request reprint Suitability of porcine chondrocyte micromass culture to model osteoarthritis in vitro
    Niels Schlichting
    Tissue Engineering Laboratory and Berlin Brandenburg Center for Regenerative Therapies BCRT, Department of Rheumatology and Clinical Immunology, Charite Universitatsmedizin Berlin, 10117 Berlin, Germany
    Mol Pharm 11:2092-105. 2014
    ..In conclusion, the porcine micromass model represents an alternative tissue platform for the evaluation of innovative substances and techniques for the treatment of OA. ..
  48. ncbi request reprint Human polymer-based cartilage grafts for the regeneration of articular cartilage defects
    M Endres
    Charite University Medicine Berlin, Department of Rheumatology, Tissue Engineering Laboratories, Berlin, Germany
    Tissue Cell 39:293-301. 2007
    ..These results suggest that human polymer-based cartilage tissue engineering grafts made of human chondrocytes, human fibrin and PGA are clinically suited for the regeneration of articular cartilage defects...
  49. doi request reprint Effect of different ascorbate supplementations on in vitro cartilage formation in porcine high-density pellet cultures
    Y Ibold
    Tissue Engineering Laboratory and Berlin Brandenburg Center for Regenerative Therapies, Department of Rheumatology and Clinical Immunology, Charite Universitatsmedizin Berlin, Tucholskystrasse 2, 10117 Berlin, Germany
    Tissue Cell 41:249-56. 2009
    ..Besides that, we could not detect a pronounced difference between ascorbate and its derivates as well as between the different concentrations...
  50. ncbi request reprint [Tissue engineering: chances and challenges for application in rheumatic diseases]
    T Haupl
    Humboldt Universitat, Tucholskystr 2, 10117 Berlin, Germany
    Z Rheumatol 62:II46-9. 2003
    ..These together with the advances in biological therapies of rheumatic diseases are the basis of new concepts, which promise application of tissue engineering also in inflammatory joint diseases...
  51. pmc Cardiac migration of endogenous mesenchymal stromal cells in patients with inflammatory cardiomyopathy
    Caroline Schmidt-Lucke
    Department of Cardiology, Charite University Medicine Berlin, Campus Benjamin Franklin, 12200 Berlin, Germany Berlin Brandenburg Center of Regenerative Therapies BCRT, 13353 Berlin, Germany Medico Academic Consultings MEDIACC, 14193 Berlin, Germany
    Mediators Inflamm 2015:308185. 2015
    ..73, P < 0.005). SDF-1α was the strongest predictor for increased MSC in EMB (P < 0.005, multivariate analysis). Conclusions. Endogenous MSC continuously migrate to the heart in patients with CMi triggered by cardiac inflammation. ..
  52. ncbi request reprint In vitro expression of cartilage-specific markers by chondrocytes on a biocompatible hydrogel: implications for engineering cartilage tissue
    M Risbud
    Tissue Engineering Laboratory, University Medical Centre, , Humboldt University of Berlin, Germany
    Cell Transplant 10:755-63. 2001
    ..This hydrogel needs to be further evaluated for its ability to support chondrocyte-specific marker expression to explore the possibility of forming a tissue resembling natural cartilage in vitro...
  53. doi request reprint Toward in situ tissue engineering: chemokine-guided stem cell recruitment
    Kristin Andreas
    Tissue Engineering Laboratory and Berlin Brandenburg Center for Regenerative Therapies, Department of Rheumatology and Clinical Immunology, Charite Universitatsmedizin Berlin, Föhrer Strasse 15, 13353 Berlin, Germany Electronic address
    Trends Biotechnol 32:483-92. 2014
    ....
  54. doi request reprint Transdifferentiation of adipogenically differentiated cells into osteogenically or chondrogenically differentiated cells: phenotype switching via dedifferentiation
    Mujib Ullah
    Tissue Engineering Laboratory and Berlin Brandenburg Center for Regenerative Therapies, Department of Rheumatology and Clinical Immunology, Charite University Medicine Berlin, Chariteplatz 1, 10117 Berlin, Germany Electronic address
    Int J Biochem Cell Biol 46:124-37. 2014
    ..An obvious characterization of differentiated cells could be helpful to understand the process of transdifferentiation. ..
  55. doi request reprint Microencapsulation and chondrogenic differentiation of human mesenchymal progenitor cells from subchondral bone marrow in Ca-alginate for cell injection
    M Endres
    TransTissue Technologies GmbH, Berlin, Germany
    Acta Biomater 6:436-44. 2010
    ..Therefore, from the cell biology point of view, Ca-alginate, an established hydrogel scaffold material is suited for regenerative therapies of cartilage defects based on the injection of progenitor cells...
  56. ncbi request reprint Biocompatible hydrogel supports the growth of respiratory epithelial cells: possibilities in tracheal tissue engineering
    M Risbud
    Tissue Engineering Laboratory, University Medical Centre, , Humboldt University of Berlin, Tucholkystrasse-2, 10117 Berlin, Germany
    J Biomed Mater Res 56:120-7. 2001
    ..This hydrogel is suitable as a culture substratum for respiratory epithelial cells and could be used as a potential candidate for coating tracheal prostheses...
  57. ncbi request reprint Hyaluronic acid and autologous synovial fluid induce chondrogenic differentiation of equine mesenchymal stem cells: a preliminary study
    A A Hegewald
    Tissue Engineering Laboratory, Department of Rheumatology, Charite University Medicine Berlin, Campus Mitte, Berlin, Germany
    Tissue Cell 36:431-8. 2004
    ....
  58. ncbi request reprint Development of a high-throughput screening assay based on the 3-dimensional pannus model for rheumatoid arthritis
    Yvonne Ibold
    Department of Bioprocess Engineering, Institute of Biotechnology, Technical University of Berlin, Germany
    J Biomol Screen 12:956-65. 2007
    ..In addition, automated cell seeding and media exchange increased the efficiency due to a reduction of labor intensity and handling time...
  59. pmc Osteogenic potential of human periosteum-derived progenitor cells in PLGA scaffold using allogeneic serum
    Yi xiong Zheng
    Department of Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China
    J Zhejiang Univ Sci B 7:817-24. 2006
    ..The results of this study support the concept that this tissue engineering method presents a promising method for creation of new bone in vivo...
  60. ncbi request reprint Specific plasma membrane protein phenotype of culture-amplified and native human bone marrow mesenchymal stem cells
    Bruno Delorme
    INSERM, Equipe ESPRI EA 3855, Faculty of Medicine, University Francois Rabelais, 10 boulevard Tonnelle, Tours, France
    Blood 111:2631-5. 2008
    ..In addition, the expression of CD73, CD146, and CD200 was down-regulated in differentiated cells. The new marker CD200, because of its specificity and immunomodulatory properties, deserves further in-depth studies...
  61. ncbi request reprint A microcarrier-based cultivation system for expansion of primary mesenchymal stem cells
    Simone Frauenschuh
    Department of Bioengineering, Institute for Biotechnology, Technical University of Berlin, Seestrasse 13 Sek GG 2, 13353 Berlin, Germany
    Biotechnol Prog 23:187-93. 2007
    ..Totaling the results, we have shown that the three-dimensional expansion of MSC on microcarriers represents a beneficial alternative to the conventional two-dimensional monolayer cultivation method...
  62. ncbi request reprint Emulsion-based synthesis of PLGA-microspheres for the in vitro expansion of porcine chondrocytes
    Franziska Gabler
    Technical University Berlin, Institute of Biotechnology, Ackerstrasse 71 76, Berlin 13355, Germany
    Biomol Eng 24:515-20. 2007
    ..The degradation of the microspheres was determined in a long-term experiment in culture medium for 3 months. Adherent cells were characterized after 3 and 5 days by FDA+EB vital staining and in SEM...
  63. ncbi request reprint Treatment of articular cartilage defects in horses with polymer-based cartilage tissue engineering grafts
    Dirk Barnewitz
    Research Center of Medical Technology and Biotechnology, Bad Langensalza, Germany
    Biomaterials 27:2882-9. 2006
    ....
  64. ncbi request reprint Regenerative treatment strategies in spinal surgery
    Aldemar Andres Hegewald
    Department of Neurosurgery, University Hospital Mannheim, University of Heidelberg, Theodor Kutzer Ufer 1 3, 68167 Mannheim, Germany
    Front Biosci 13:1507-25. 2008
    ..Here, additional regenerative interventions would have a preventive intention, whereas interventions for painful degenerative disc disease as an alternative to spinal fusion or disc arthroplasty would be a curative approach...