B Hoffmann

Summary

Country: Germany

Publications

  1. pmc High resolution melting analysis: rapid and precise characterisation of recombinant influenza A genomes
    Donata Kalthoff
    Institute of Diagnostic Virology, Friedrich Loeffler Institut, Südufer 10, Greifswald Insel Riems, 17493, Germany
    Virol J 10:284. 2013
  2. pmc Species determination of Culicoides biting midges via peptide profiling using matrix-assisted laser desorption ionization mass spectrometry
    Katrin R Uhlmann
    Department of Proteomics, Helmholtz Centre for Environmental Research UFZ, 04318 Leipzig, Germany
    Parasit Vectors 7:392. 2014
  3. pmc Towards the PCR-based identification of Palaearctic Culicoides biting midges (Diptera: Ceratopogonidae): results from an international ring trial targeting four species of the subgenus Avaritia
    Claire Garros
    CIRAD, UMR15 CMAEE, 34398 Montpellier, France
    Parasit Vectors 7:223. 2014
  4. pmc Tick-borne encephalitis virus (TBEV) - findings on cross reactivity and longevity of TBEV antibodies in animal sera
    Christine Klaus
    Institute of Bacterial Infections and Zoonoses, Friedrich Loeffler Institut, Naumburger Str, 96a, D 07743 Jena, Germany
    BMC Vet Res 10:78. 2014
  5. pmc Development and validation of rapid magnetic particle based extraction protocols
    Andrea Aebischer
    Institute of Diagnostic Virology, Friedrich Loeffler Institut, Suedufer 10, 17493 Greifwald Insel Riems, Germany
    Virol J 11:137. 2014
  6. pmc A step forward in molecular diagnostics of lyssaviruses--results of a ring trial among European laboratories
    Melina Fischer
    Institute of Diagnostic Virology, Friedrich Loeffler Institut, Greifswald Insel Riems, Germany
    PLoS ONE 8:e58372. 2013
  7. pmc Development of a pan-Simbu real-time reverse transcriptase PCR for the detection of Simbu serogroup viruses and comparison with SBV diagnostic PCR systems
    Melina Fischer
    Institute of Diagnostic Virology, Friedrich Loeffler Institut, Südufer 10, D 17493, Greifswald Insel Riems, Germany
    Virol J 10:327. 2013
  8. pmc Schmallenberg virus challenge models in cattle: infectious serum or culture-grown virus?
    Kerstin Wernike
    Institute of Diagnostic Virology, Friedrich Loeffler Institut FLI, Suedufer 10, Greifswald, Insel Riems, 17493, Germany
    Vet Res 43:84. 2012
  9. pmc PCR identification of culicoid biting midges (Diptera, Ceratopogonidae) of the Obsoletus complex including putative vectors of bluetongue and Schmallenberg viruses
    Kathrin Lehmann
    Federal Research Institute for Animal Health, Greifswald Insel Riems, Germany
    Parasit Vectors 5:213. 2012
  10. doi request reprint Porcine reproductive and respiratory syndrome virus: interlaboratory ring trial to evaluate real-time reverse transcription polymerase chain reaction detection methods
    Kerstin Wernike
    Institute of Diagnostic Virology, Friedrich Loeffler Institut, Südufer 10, 17493 Greifswald Insel Riems, Germany
    J Vet Diagn Invest 24:855-66. 2012

Collaborators

Detail Information

Publications43

  1. pmc High resolution melting analysis: rapid and precise characterisation of recombinant influenza A genomes
    Donata Kalthoff
    Institute of Diagnostic Virology, Friedrich Loeffler Institut, Südufer 10, Greifswald Insel Riems, 17493, Germany
    Virol J 10:284. 2013
    ..Because the reverse genetics of segmented influenza A viruses allows the generation of numerous influenza A virus reassortants within a short time, methods for the rapid selection of the correct recombinants are very useful...
  2. pmc Species determination of Culicoides biting midges via peptide profiling using matrix-assisted laser desorption ionization mass spectrometry
    Katrin R Uhlmann
    Department of Proteomics, Helmholtz Centre for Environmental Research UFZ, 04318 Leipzig, Germany
    Parasit Vectors 7:392. 2014
    ..The aim of this proof-of-principle study was to develop a simple MALDI-MS based method to differentiate Culicoides from different species by peptide patterns with the additional option of identifying discriminating peptides...
  3. pmc Towards the PCR-based identification of Palaearctic Culicoides biting midges (Diptera: Ceratopogonidae): results from an international ring trial targeting four species of the subgenus Avaritia
    Claire Garros
    CIRAD, UMR15 CMAEE, 34398 Montpellier, France
    Parasit Vectors 7:223. 2014
    ..Within the western Palaearctic region, the main suspected vector species, C. obsoletus, C. scoticus, C. dewulfi and C. chiopterus, have similar wing patterns, which makes it difficult to separate and identify them correctly...
  4. pmc Tick-borne encephalitis virus (TBEV) - findings on cross reactivity and longevity of TBEV antibodies in animal sera
    Christine Klaus
    Institute of Bacterial Infections and Zoonoses, Friedrich Loeffler Institut, Naumburger Str, 96a, D 07743 Jena, Germany
    BMC Vet Res 10:78. 2014
    ....
  5. pmc Development and validation of rapid magnetic particle based extraction protocols
    Andrea Aebischer
    Institute of Diagnostic Virology, Friedrich Loeffler Institut, Suedufer 10, 17493 Greifwald Insel Riems, Germany
    Virol J 11:137. 2014
    ..However, methods enabling rapid and simple nucleic acid extraction also under resource-limited settings are still scarce...
  6. pmc A step forward in molecular diagnostics of lyssaviruses--results of a ring trial among European laboratories
    Melina Fischer
    Institute of Diagnostic Virology, Friedrich Loeffler Institut, Greifswald Insel Riems, Germany
    PLoS ONE 8:e58372. 2013
    ..To improve the knowledge about the diagnostic performance proficiency testing at an international level is recommended before using lyssavirus molecular diagnostics e.g. for confirmatory testing...
  7. pmc Development of a pan-Simbu real-time reverse transcriptase PCR for the detection of Simbu serogroup viruses and comparison with SBV diagnostic PCR systems
    Melina Fischer
    Institute of Diagnostic Virology, Friedrich Loeffler Institut, Südufer 10, D 17493, Greifswald Insel Riems, Germany
    Virol J 10:327. 2013
    ..In view of the current situation and the possible emergence of further Simbu serogroup members, a pan-Simbu real-time reverse transcriptase (RT) PCR system for the reliable detection of Simbu serogroup viruses should be developed...
  8. pmc Schmallenberg virus challenge models in cattle: infectious serum or culture-grown virus?
    Kerstin Wernike
    Institute of Diagnostic Virology, Friedrich Loeffler Institut FLI, Suedufer 10, Greifswald, Insel Riems, 17493, Germany
    Vet Res 43:84. 2012
    ..83 infectious doses per mL. Hence, serum-borne virus that was only passaged in the natural host is a suitable option for a standardized SBV infection model...
  9. pmc PCR identification of culicoid biting midges (Diptera, Ceratopogonidae) of the Obsoletus complex including putative vectors of bluetongue and Schmallenberg viruses
    Kathrin Lehmann
    Federal Research Institute for Animal Health, Greifswald Insel Riems, Germany
    Parasit Vectors 5:213. 2012
    ..obsoletus, C. scoticus, C. chiopterus) plus the isomorphic species C. dewulfi...
  10. doi request reprint Porcine reproductive and respiratory syndrome virus: interlaboratory ring trial to evaluate real-time reverse transcription polymerase chain reaction detection methods
    Kerstin Wernike
    Institute of Diagnostic Virology, Friedrich Loeffler Institut, Südufer 10, 17493 Greifswald Insel Riems, Germany
    J Vet Diagn Invest 24:855-66. 2012
    ..In summary, a major problem in PRRSV diagnostics by RT-qPCR is false-negative results. To achieve maximum safety in the molecular diagnosis of PRRSV, the combined usage of different assays or kits is highly recommended...
  11. doi request reprint Fatalities in wild goats in Kurdistan associated with Peste des Petits Ruminants virus
    B Hoffmann
    Institute of Diagnostic Virology, Friedrich Loeffler Institut FLI, Federal Research Institute for Animal Health, Greifswald Insel Riems, Germany
    Transbound Emerg Dis 59:173-6. 2012
    ..It is therefore recommended to continue with the campaign. Conversely, there is no justification for the use of force to keep the endangered wild goats away from domestic flocks...
  12. pmc Avian influenza virus monitoring in wintering waterbirds in Iran, 2003-2007
    Sasan R Fereidouni
    Friedrich Loeffler Institut, Insel Riems, Germany
    Virol J 7:43. 2010
    ..Samples originated from 6 different provinces representative for the 15 most important wintering sites of migratory waterbirds in Iran...
  13. ncbi request reprint Fish rhabdoviruses: molecular epidemiology and evolution
    B Hoffmann
    Friedrich Loeffler Institut, Federal Research Institute for Animal Health, 17493 Greifswald Insel Riems, Germany
    Curr Top Microbiol Immunol 292:81-117. 2005
    ....
  14. doi request reprint Classical swine fever virus detection: results of a real-time reverse transcription polymerase chain reaction ring trial conducted in the framework of the European network of excellence for epizootic disease diagnosis and control
    Bernd Hoffmann
    Friedrich Loeffler Institut, Institute of Diagnostic Virology, Suedufer 10, 17493 Greifswald, Riems Island, Germany
    J Vet Diagn Invest 23:999-1004. 2011
    ..In conclusion, the ring trial showed reliability of classical swine fever diagnosis on an international level and helped to optimize CSFV-specific RT-PCR diagnostics...
  15. ncbi request reprint Phylogenetic analyses of highly pathogenic avian influenza virus isolates from Germany in 2006 and 2007 suggest at least three separate introductions of H5N1 virus
    E Starick
    Institute of Diagnostic Virology, Friedrich Loeffler Institute, 17493 Greifswald Insel Riems, Germany
    Vet Microbiol 128:243-52. 2008
    ..The viruses detected in 2007 most likely represent another new incursion from an as yet unknown source...
  16. doi request reprint Comparative safety study of three inactivated BTV-8 vaccines in sheep and cattle under field conditions
    J Gethmann
    Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Seestrasse 55, 16868 Wusterhausen, Germany
    Vaccine 27:4118-26. 2009
    ..Among cattle, neither fever, nor a decrease in milk production and only temporary low-grade local reactions were observed. Overall, our results demonstrate a high level of safety of all vaccines tested...
  17. ncbi request reprint A universal heterologous internal control system for duplex real-time RT-PCR assays used in a detection system for pestiviruses
    B Hoffmann
    Institute of Diagnostic Virology, Friedrich Loeffler Institut, Boddenblick 5a, D 17493 Greifswald Insel Riems, Germany
    J Virol Methods 136:200-9. 2006
    ....
  18. doi request reprint Schmallenberg virus
    K Wernike
    Institute of Diagnostic Virology, Friedrich Loeffler Institut FLI, Greifswald Insel Riems, Germany
    Dev Biol (Basel) 135:175-82. 2013
    ..Diagnostic assays like real-time RT-PCR and antibody ELISA are now available; and substantial knowledge of this virus has been gathered quickly. However, further research and exchange of information are essential...
  19. ncbi request reprint Pathology of natural infections by H5N1 highly pathogenic avian influenza virus in mute (Cygnus olor) and whooper (Cygnus cygnus) swans
    J P Teifke
    Federal Research Institute for Animal Health, Boddenblick 5a, 17493 Greifswald Insel Riems, Germany
    Vet Pathol 44:137-43. 2007
    ..More investigations are necessary to clarify the mechanisms of the final circulatory failure, lung edema, and rapid death of the swans...
  20. doi request reprint Tick-borne encephalitis (TBE) virus prevalence and virus genome characterization in field-collected ticks (Ixodes ricinus) from risk, non-risk and former risk areas of TBE, and in ticks removed from humans in Germany
    C Klaus
    Institute of Bacterial Infections and Zoonoses, Friedrich Loeffler Institute, Jena, Germany
    Clin Microbiol Infect 16:238-44. 2010
    ..However, ticks were also collected from volunteers living in Bavaria. Three of 239 ticks from this collection were positive for TBEV genome and two genetically distinct TBEV strains were detected and characterized...
  21. ncbi request reprint Diagnostic evaluation of a real-time RT-PCR assay for routine diagnosis of classical swine fever in wild boar
    K Depner
    Federal Research Centre for Virus Diseases of Animals, Boddenblick 5a, 17498 Insel Riems Greifswald, Germany
    J Vet Med B Infect Dis Vet Public Health 53:317-20. 2006
    ..The use of real-time RT-PCR instead of antigen-ELISA and virus isolation as a routine tool for control and eradication of CSF in wild boar populations is recommended...
  22. doi request reprint High Prevalence of Highly Variable Atypical Porcine Pestiviruses Found in Germany
    M Beer
    Friedrich Loeffler Institut, Greifswald Insel Riems, Germany
    Transbound Emerg Dis . 2016
    ..Subsequent screening of the German pig sector showed a high prevalence of APPV with high variability among strains. First indication of a cell culture isolate is provided which will allow further investigations like pathogenesis studies...
  23. ncbi request reprint Validation of a real-time RT-PCR assay for sensitive and specific detection of classical swine fever
    B Hoffmann
    Institute of Diagnostic Virology, Friedrich Loeffler Institut, Boddenblick 5a, D 17493 Greifswald Insel Riems, Germany
    J Virol Methods 130:36-44. 2005
    ..The primer-probe combination selected was strictly CSFV-specific and no amplification was observed in all non-CSFV pestiviruses tested...
  24. pmc Improved safety for molecular diagnosis of classical rabies viruses by use of a TaqMan real-time reverse transcription-PCR "double check" strategy
    B Hoffmann
    Institute of Diagnostic Virology, Südufer 10, 17493 Greifswald Insel Riems, Germany
    J Clin Microbiol 48:3970-8. 2010
    ....
  25. doi request reprint Characterization of pseudorabies virus of wild boar origin from Europe
    T Muller
    Institute of Epidemiology, Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Wusterhausen, Germany
    Epidemiol Infect 138:1590-600. 2010
    ....
  26. ncbi request reprint Persistence of viral RNA in rabbits which overcome an experimental RHDV infection detected by a highly sensitive multiplex real-time RT-PCR
    A Gall
    Friedrich Loeffler Institut, Institute of Diagnostic Virology, Boddenblick 5a, 17493 Greifswald Insel Riems, Germany
    Vet Microbiol 120:17-32. 2007
    ..Therefore, further experiments are necessary to prove that the persistence of RNA is linked with the persistence of infectious virus particles...
  27. doi request reprint Schmallenberg Virus Infection Diagnosis: Results of a German Proficiency Trial
    K Wernike
    Institute of Diagnostic Virology, Friedrich Loeffler Institut, Greifswald Insel Riems, Germany
    Transbound Emerg Dis . 2016
    ..In conclusion, the here described German SBV proficiency test demonstrated that the available test systems allowed reliable SBV diagnostics in standard veterinary laboratories when recommended and approved assays are used...
  28. doi request reprint Twenty years of active bat rabies surveillance in Germany: a detailed analysis and future perspectives
    J Schatz
    Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Institute of Molecular Biology, WHO Collaborating Centre for Rabies Surveillance and Research, Greifswald Insel Riems, Germany
    Epidemiol Infect 142:1155-66. 2014
    ....
  29. doi request reprint Clinical course and pathology in rats (Rattus norvegicus) after experimental cowpox virus infection by percutaneous and intranasal application
    A Breithaupt
    Friedrich Loeffler Institut, Federal Research Institute for Animal Health, Südufer 10, 17493 Greifswald Insel, Riems, Germany
    Vet Pathol 49:941-9. 2012
    ..This is the first histomorphological and immunohistochemical analysis of CPXV in rats after experimental infection...
  30. doi request reprint Detection of a Novel Bovine Astrovirus in a Cow with Encephalitis
    K Schlottau
    Friedrich Loeffler Institut, Institute of Diagnostic Virology, Greifswald Insel Riems, Germany
    Transbound Emerg Dis 63:253-9. 2016
    ..In conclusion, while astroviruses so far were mainly found in relation to gastroenteritis in animals and humans, recently detected astrovirus infections were also related to encephalitis...
  31. doi request reprint Tick-borne encephalitis virus (TBEV) infection in horses: clinical and laboratory findings and epidemiological investigations
    C Klaus
    Institute of Bacterial Infections and Zoonoses, Friedrich Loeffler Institut, Naumburger Str 96a, D 07743 Jena, Germany
    Vet Microbiol 163:368-72. 2013
    ..The suitability of horses as sentinels for the detection of natural TBEV foci supported by subsequent, targeted tick collection as well as the clinical importance of TBEV infection in horses are discussed...
  32. ncbi request reprint Case report: the significance of genotyping for the epidemiological tracing of classical swine fever (CSF)
    K R Depner
    Friedrich Loeffler lnstitut, Insel Riems Greifswald, Germany
    Dtsch Tierarztl Wochenschr 113:159-62. 2006
    ..This case is decribed in detail. The epidemiological significance of different diagnostic methods is discussed, in particular the genetic typing of CSF virus isolates...
  33. ncbi request reprint [Social inequality and noise pollution by traffic in the living environment--an analysis by the German Federal Health Survey (Bundesgesundheitssurvey)]
    B Hoffmann
    Institut für Sozialmedizin und Gesundheitsökonomie, Medizinische Fakultat, Otto von Guericke Universitat Magdeburg
    Gesundheitswesen 65:393-401. 2003
    ....
  34. ncbi request reprint Experimental chronic arthritis and granulomatous inflammation induced by bifidobacterium cell walls
    X Zhang
    Department of Medical Microbiology, Turku Immunology Centre, University of Turku, Turku, Finland
    Scand J Immunol 54:171-9. 2001
    ..All these in comparison to the other strain used. Such characteristics are associated with the capacity to induce chronic arthritis, but it remains open how crucial they are for the granuloma formation...
  35. ncbi request reprint Estrogen receptor beta (ERbeta) is expressed differently from ERalpha in bovine placentomes
    G Schuler
    Klinik für Geburtshilfe, Gynäkologie und Andrologie der Gross und Kleintiere mit Tierärztlicher Ambulanz, Justus Liebig Universitat, Giessen, Germany
    Exp Clin Endocrinol Diabetes 113:107-14. 2005
    ....
  36. ncbi request reprint Expression of oestrogen receptors alpha and beta and of aromatase in the testis of immature and mature boars
    H M Mutembei
    Clinic for Obstetrics, Gynaecology and Andrology of Large and Small Animals, Justus Liebig University, Giessen, Germany
    Reprod Domest Anim 40:228-36. 2005
    ..P450 aromatase immunoreaction was restricted to the cytoplasm of Leydig cells. These findings suggest that testicular ER may be important factors contributing to onset and maintenance of spermatogenesis in the boar...
  37. ncbi request reprint Fibroblast growth factor (FGF)-1, FGF2, FGF7 and FGF receptors are uniformly expressed in trophoblast giant cells during restricted trophoblast invasion in cows
    C Pfarrer
    Institute for Veterinary Anatomy, Histology and Embryology, Justus Liebig University, D 35392 Giessen, Germany
    Placenta 27:758-70. 2006
    ....
  38. ncbi request reprint Expression of cyclooxygenase-II (COX-II) and 20alpha-hydroxysteroid dehydrogenase (20alpha-HSD)/prostaglandin F-synthase (PGFS) in bovine placentomes: implications for the initiation of parturition in cattle
    G Schuler
    Clinic for Obstetrics, Gynecology and Andrology of Large and Small Animals, Faculty of Veterinary Medicine, Justus Liebig University, D 35392 Giessen, Germany
    Placenta 27:1022-9. 2006
    ....
  39. ncbi request reprint A virological survey in migrating waders and other waterfowl in one of the most important resting sites of Germany
    A Hlinak
    State Laboratory Brandenburg, Frankfurt Oder, D 15236 Frankfurt Oder, Germany
    J Vet Med B Infect Dis Vet Public Health 53:105-10. 2006
    ..These results confirm that the restricted resting sites in Western Europe must be considered as important locations for the intra- and interspecies transmission of avian viruses...
  40. ncbi request reprint [Ultrasound education by simulator training high acceptance by ultrasound trainees of all qualification levels]
    C Terkamp
    Abteilung Gastroenterologie, Hepatologie und Endokrinologie, Zentrum Innere Medizin, Medizinische Hochschule Hannover
    Praxis (Bern 1994) 95:809-13. 2006
    ..98.5% whished to have further ultrasound simulator courses to be developed and 95.3% of the participants opted for the integration of simulator training into the conventional ultrasound education...
  41. ncbi request reprint Foot-and-mouth disease virus: a first inter-laboratory comparison trial to evaluate virus isolation and RT-PCR detection methods
    N P Ferris
    Institute for Animal Health, Pirbright Laboratory, Ash Road, Pirbright, Woking, Surrey, UK
    Vet Microbiol 117:130-40. 2006
    ....
  42. ncbi request reprint Age-dependent expression and localization of the progesterone receptor in the boar testis
    C Kohler
    Clinic for Obstetrics, Gynaecology and Andrology of Large and Small Animals, Justus Liebig University, Giessen, Germany
    Reprod Domest Anim 42:1-5. 2007
    ..In both groups also few peritubular myoid cells stained positive. It is hypothesized that Leydig cell-derived progesterone plays a functional role in spermatogoniogenesis in a synergistic manner with Leydig cell-derived oestrogens...
  43. ncbi request reprint Residential exposure to traffic is associated with coronary atherosclerosis
    B Hoffmann
    Institute for Medical Informatics, Biometry and Epidemiology, University Hospital Essen, Hufelandstrasse 55, 45122 Essen, Germany
    Circulation 116:489-96. 2007
    ..5) air pollution may accelerate the development and progression of atherosclerosis. We investigated the associations of long-term residential exposure to traffic and fine particulate matter with the degree of coronary atherosclerosis...