Lutz Wiehlmann

Summary

Affiliation: Hannover Medical School
Country: Germany

Publications

  1. doi request reprint Impact of sample processing on human airways microbial metagenomes
    Lutz Wiehlmann
    Clinical Research Group, OE 6711, Medizinische Hochschule Hannover, D 30625 Hannover, Germany Core Unit Next Generation Sequencing, Medizinische Hochschule Hannover, D 30625 Hannover, Germany Institute for Human Genetics, Medizinische Hochschule Hannover, D 30625 Hannover, Germany Electronic address
    J Biotechnol . 2017
  2. pmc THOC5, a member of the mRNA export complex, contributes to processing of a subset of wingless/integrated (Wnt) target mRNAs and integrity of the gut epithelial barrier
    Shashank Saran
    Institut fuer Biochemie, Medizinische Hochschule Hannover, OE4310 Carl Neuberg Str, 1, Hannover D 30623, Germany
    BMC Cell Biol 14:51. 2013
  3. pmc Molecular typing and epidemiological investigation of clinical populations of Pseudomonas aeruginosa using an oligonucleotide-microarray
    Annalisa Ballarini
    Centre for Integrative Biology, University of Trento, Trento, Italy
    BMC Microbiol 12:152. 2012
  4. pmc Population structure of Pseudomonas aeruginosa
    Lutz Wiehlmann
    Klinische Forschergruppe, OE 6710, Medizinische Hochschule Hannover, Carl Neuberg Strasse 1, D 30625 Hannover, Germany
    Proc Natl Acad Sci U S A 104:8101-6. 2007
  5. pmc Multiple roles of Pseudomonas aeruginosa TBCF10839 PilY1 in motility, transport and infection
    Yu Sing Tammy Bohn
    Klinische Forschergruppe, Medizinische Hochschule Hannover, Carl Neuberg Strasse, Hannover, Germany
    Mol Microbiol 71:730-47. 2009
  6. pmc Genome diversity of Pseudomonas aeruginosa PAO1 laboratory strains
    Jens Klockgether
    Klinische Forschergruppe, Medizinische Hochschule Hannover, Hannover, Germany
    J Bacteriol 192:1113-21. 2010
  7. doi request reprint Intraclonal diversity of the Pseudomonas aeruginosa cystic fibrosis airway isolates TBCF10839 and TBCF121838: distinct signatures of transcriptome, proteome, metabolome, adherence and pathogenicity despite an almost identical genome sequence
    Jens Klockgether
    Klinische Forschergruppe, Zentrum Biochemie und Zentrum Kinder und Jugendmedizin, OE 6710, D 30625 Hannover, Germany
    Environ Microbiol 15:191-210. 2013
  8. doi request reprint Transcript profiling of the Pseudomonas aeruginosa genomic islands PAGI-2 and pKLC102
    Jens Klockgether
    Klinische Forschergruppe, OE 6710, Medizinische Hochschule Hannover, D 30625 Hannover, Germany
    Microbiology 154:1599-604. 2008
  9. ncbi request reprint Functional genomics of Pseudomonas aeruginosa to identify habitat-specific determinants of pathogenicity
    Lutz Wiehlmann
    Klinische Forschergruppe, Abteilung Pädiatrische Pneumologie und Neonatologie, OE 6710, Medizinische Hochschule Hannover, Carl Neuberg Strass1, D 30625 Hannover, Germany
    Int J Med Microbiol 297:615-23. 2007
  10. doi request reprint Pseudomonas aeruginosa population biology in chronic obstructive pulmonary disease
    Elza Rakhimova
    Klinische Forschergruppe, Abteilung für Pädiatrische Pneumologie und Neonatologie, OE 6710, Medizinische Hochschule Hannover, D 30625 Hannover, Germany
    J Infect Dis 200:1928-35. 2009

Collaborators

Detail Information

Publications32

  1. doi request reprint Impact of sample processing on human airways microbial metagenomes
    Lutz Wiehlmann
    Clinical Research Group, OE 6711, Medizinische Hochschule Hannover, D 30625 Hannover, Germany Core Unit Next Generation Sequencing, Medizinische Hochschule Hannover, D 30625 Hannover, Germany Institute for Human Genetics, Medizinische Hochschule Hannover, D 30625 Hannover, Germany Electronic address
    J Biotechnol . 2017
    ..proliferated so that the total number of bacterial reads increased at the expense of human reads. We recommend that samples for metagenome analysis should be immediately processed or frozen at -80°C...
  2. pmc THOC5, a member of the mRNA export complex, contributes to processing of a subset of wingless/integrated (Wnt) target mRNAs and integrity of the gut epithelial barrier
    Shashank Saran
    Institut fuer Biochemie, Medizinische Hochschule Hannover, OE4310 Carl Neuberg Str, 1, Hannover D 30623, Germany
    BMC Cell Biol 14:51. 2013
    ..Here we interrogate THOC5 function in cell differentiation beyond the hematopoietic system and study pathological changes caused by THOC5 deficiency...
  3. pmc Molecular typing and epidemiological investigation of clinical populations of Pseudomonas aeruginosa using an oligonucleotide-microarray
    Annalisa Ballarini
    Centre for Integrative Biology, University of Trento, Trento, Italy
    BMC Microbiol 12:152. 2012
    ..aeruginosa genome. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were employed as reference genotyping techniques to estimate the ArrayTube resolution power...
  4. pmc Population structure of Pseudomonas aeruginosa
    Lutz Wiehlmann
    Klinische Forschergruppe, OE 6710, Medizinische Hochschule Hannover, Carl Neuberg Strasse 1, D 30625 Hannover, Germany
    Proc Natl Acad Sci U S A 104:8101-6. 2007
    ..Thus, the P. aeruginosa genome is made up of clone-typical segments in core and accessory genome and of blocks in the core with unrestricted gene flow in the population...
  5. pmc Multiple roles of Pseudomonas aeruginosa TBCF10839 PilY1 in motility, transport and infection
    Yu Sing Tammy Bohn
    Klinische Forschergruppe, Medizinische Hochschule Hannover, Carl Neuberg Strasse, Hannover, Germany
    Mol Microbiol 71:730-47. 2009
    ..P. aeruginosa PilY1 is not an adhesin on the pilus tip, but assists in pilus biogenesis, twitching motility, secretion of secondary metabolites and in the control of cell density in the bacterial population...
  6. pmc Genome diversity of Pseudomonas aeruginosa PAO1 laboratory strains
    Jens Klockgether
    Klinische Forschergruppe, Medizinische Hochschule Hannover, Hannover, Germany
    J Bacteriol 192:1113-21. 2010
    ..High-throughput genome resequencing will resolve more cases and could become a proper quality control for strain collections...
  7. doi request reprint Intraclonal diversity of the Pseudomonas aeruginosa cystic fibrosis airway isolates TBCF10839 and TBCF121838: distinct signatures of transcriptome, proteome, metabolome, adherence and pathogenicity despite an almost identical genome sequence
    Jens Klockgether
    Klinische Forschergruppe, Zentrum Biochemie und Zentrum Kinder und Jugendmedizin, OE 6710, D 30625 Hannover, Germany
    Environ Microbiol 15:191-210. 2013
    ..Microevolution in P. aeruginosa apparently can generate novel complex traits by few or even single mutations provided that predisposing mutational events had occurred before in the clonal lineage...
  8. doi request reprint Transcript profiling of the Pseudomonas aeruginosa genomic islands PAGI-2 and pKLC102
    Jens Klockgether
    Klinische Forschergruppe, OE 6710, Medizinische Hochschule Hannover, D 30625 Hannover, Germany
    Microbiology 154:1599-604. 2008
    ....
  9. ncbi request reprint Functional genomics of Pseudomonas aeruginosa to identify habitat-specific determinants of pathogenicity
    Lutz Wiehlmann
    Klinische Forschergruppe, Abteilung Pädiatrische Pneumologie und Neonatologie, OE 6710, Medizinische Hochschule Hannover, Carl Neuberg Strass1, D 30625 Hannover, Germany
    Int J Med Microbiol 297:615-23. 2007
    ....
  10. doi request reprint Pseudomonas aeruginosa population biology in chronic obstructive pulmonary disease
    Elza Rakhimova
    Klinische Forschergruppe, Abteilung für Pädiatrische Pneumologie und Neonatologie, OE 6710, Medizinische Hochschule Hannover, D 30625 Hannover, Germany
    J Infect Dis 200:1928-35. 2009
    ..We report on the molecular epidemiology and population biology of P. aeruginosa in COPD...
  11. pmc Molecular epidemiology of chronic Pseudomonas aeruginosa airway infections in cystic fibrosis
    Nina Cramer
    Clinical Research Group, Clinic for Pediatric Pneumology, Allergology and Neonatology, Hanover Medical School, Hanover, Germany
    PLoS ONE 7:e50731. 2012
    ....
  12. doi request reprint Effective prevention of Pseudomonas aeruginosa cross-infection at a cystic fibrosis centre - results of a 10-year prospective study
    Lutz Wiehlmann
    Klinische Forschergruppe, Medizinische Hochschule Hannover, Germany
    Int J Med Microbiol 302:69-77. 2012
    ..It can be concluded that cross-infection with P. aeruginosa was prevented with simple hygienic measures at a CF centre that had experienced local outbreaks of nosocomial spread among unrelated patients in the past...
  13. ncbi request reprint GeneChip expression analysis of the VqsR regulon of Pseudomonas aeruginosa TB
    Mario Juhas
    Clinical Research Group OE 6711, Hannover Medical School, Carl Neuberg Strasse 1, 30625 Hannover, Germany
    FEMS Microbiol Lett 242:287-95. 2005
    ..The expression of a large group of metabolic genes, ECF sigma factors as well as of many quorum-sensing genes previously not assigned to VqsR-regulon was found to be affected by the disruption of vqsR...
  14. pmc Intraclonal genome diversity of the major Pseudomonas aeruginosa clones C and PA14
    Sebastian Fischer
    Clinical Research Group Molecular Pathology of Cystic Fibrosis and Pseudomonas Genomics, OE 6710, Hannover Medical School, Hannover, Germany
    Environ Microbiol Rep 8:227-34. 2016
    ..Each strain carried a large cargo of unique genes. The two dominant worldwide distributed P. aeruginosa clones combine an almost invariant core with the flexible gain and loss of genetic elements that spread by horizontal transfer. ..
  15. pmc Fitness of isogenic colony morphology variants of Pseudomonas aeruginosa in murine airway infection
    Elza Rakhimova
    Clinical Research Group, OE6710, Hanover Medical School, Hanover, Germany
    PLoS ONE 3:e1685. 2008
    ....
  16. doi request reprint Multilocus amplicon sequencing of Pseudomonas aeruginosa cystic fibrosis airways isolates collected prior to and after early antipseudomonal chemotherapy
    Sebastian Fischer
    Clinical Research Group Molecular Pathology of Cystic Fibrosis, Clinic for Paediatric Pneumology, Allergology and Neonatology, OE 6710, Hannover Medical School, Hannover, Germany
    J Cyst Fibros . 2016
    ..Early antimicrobial chemotherapy can prevent or at least delay chronic cystic fibrosis (CF) airways infections with Pseudomonas aeruginosa...
  17. pmc The cystic fibrosis lower airways microbial metagenome
    Patricia Morán Losada
    Clinic for Paediatric Pneumology, Allergology and Neonatology, OE 6710, Hannover Medical School, Hannover, Germany
    ERJ Open Res 2:. 2016
    ..The rare clones constitute a low copy genetic resource that could rapidly expand as a response to habitat alterations, such as antimicrobial chemotherapy or invasion of novel microbes...
  18. pmc Molecular Epidemiology of Mutations in Antimicrobial Resistance Loci of Pseudomonas aeruginosa Isolates from Airways of Cystic Fibrosis Patients
    Leonie Greipel
    Clinical Research Group Molecular Pathology of Cystic Fibrosis, Clinic for Pediatric Pneumology, Allergology and Neonatology, Hannover Medical School, Hannover, Germany
    Antimicrob Agents Chemother 60:6726-6734. 2016
    ..Private and de novo mutations seem to play a pivotal role in the response of P. aeruginosa populations to the antimicrobial load and the individual CF host...
  19. pmc Diversity of the abundant pKLC102/PAGI-2 family of genomic islands in Pseudomonas aeruginosa
    Jens Klockgether
    Klinische Forschergruppe, OE 6710, Medizinische Hochschule Hannover, Carl Neuberg Str 1, D 30625 Hannover, Germany
    J Bacteriol 189:2443-59. 2007
    ..This evolutionarily ancient family of genomic islands retained its oligonucleotide signature during horizontal spread within and among taxa...
  20. doi request reprint Clonal epidemiology of Pseudomonas aeruginosa in cystic fibrosis
    Nina Cramer
    Klinik für Pädiatrische Pneumologie und Neonatologie, Medizinische Hochschule Hannover, Germany
    Int J Med Microbiol 300:526-33. 2010
    ..Highly transmissible epidemic strains, however, that emerge within a clonal lineage remain a major, still unresolved health threat for the CF community...
  21. ncbi request reprint Global regulation of quorum sensing and virulence by VqsR in Pseudomonas aeruginosa
    Mario Juhas
    Clinical Research Group, Hannover Medical School, Carl Neuberg Str 1, 30625 Hannover, Germany
    Microbiology 150:831-41. 2004
    ..The protein encoded by PA2591 regulates several traits of pathogenicity; hence, the name vqsR ('virulence and quorum-sensing regulator') was assigned to PA2591...
  22. doi request reprint In vivo imaging of bioluminescent Pseudomonas aeruginosa in an acute murine airway infection model
    Antje Munder
    Clinical Research Group, Hannover Medical School, Clinic for Pediatric Pneumology, Allergology and Neonatology, Hannover, Germany
    Pathog Dis 72:74-7. 2014
    ....
  23. pmc Gene islands integrated into tRNA(Gly) genes confer genome diversity on a Pseudomonas aeruginosa clone
    Karen D Larbig
    Klinische Forschergruppe, Medizinische Hochschule Hannover, Hannover, Germany
    J Bacteriol 184:6665-80. 2002
    ....
  24. doi request reprint Habitat-associated skew of clone abundance in the Pseudomonas aeruginosa population
    Lutz Wiehlmann
    Clinical Research Group, Molecular Pathology of Cystic Fibrosis and Pseudomonas Genomics, Hannover Medical School, Hannover, Germany
    Environ Microbiol Rep 7:955-60. 2015
    ..The P. aeruginosa population consists of major clones that are just as versatile in their habitat and geographic origin as the whole species and of minor clones with preference for a peculiar niche. ..
  25. doi request reprint Visualization of Pseudomonas genomic structure by abundant 8-14mer oligonucleotides
    Colin F Davenport
    Klinische Forschergruppe, OE 6711, Medizinische Hochschule Hannover, Hanover, Germany
    Environ Microbiol 11:1092-104. 2009
    ..In the future of next-generation ultra-high throughput DNA sequencing, the composition of bacterial metagenomes may be quantified by scanning the primary sequence reads for these 8-14mer markers...
  26. ncbi request reprint Sequence diversity of the mucABD locus in Pseudomonas aeruginosa isolates from patients with cystic fibrosis
    Alessandra Bragonzi
    Institute of Medical Microbiology and Hygiene, Universitatsklinikum Tubingen, Tubingen, Germany
    Microbiology 152:3261-9. 2006
    ..The exceptions point to intragenic mosaicism and interclonal recombination as major forces for intraclonal evolution at the mucABD locus...
  27. ncbi request reprint Characterization of poxB, a chromosomal-encoded Pseudomonas aeruginosa oxacillinase
    Kok Fai Kong
    Department of Biological Sciences, Florida International University, University Park, Miami, FL 33199, USA
    Gene 358:82-92. 2005
    ..Our studies show that P. aeruginosa possesses two beta-lactamases, AmpC and PoxB, which contribute to its resistance against a wide spectrum of beta-lactam antibiotics...
  28. pmc The neglected intrinsic resistome of bacterial pathogens
    Alicia Fajardo
    Departamento de Biotecnologia Microbiana, Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas CSIC, Cantoblanco, Madrid, Spain
    PLoS ONE 3:e1619. 2008
    ..Our results demonstrate that antibiotic resistance genes are not merely protective shields and offer a more comprehensive view of the role of antibiotic resistance genes in the clinic and in nature...
  29. pmc Crystal structure of the electron transfer complex rubredoxin rubredoxin reductase of Pseudomonas aeruginosa
    Gregor Hagelueken
    Molecular Host Pathogen Interactions, Division of Structural Biology, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, D 38124 Braunschweig, Germany
    Proc Natl Acad Sci U S A 104:12276-81. 2007
    ..Only a small number of direct interactions govern mutual recognition of RdxR and Rdx, corroborating the transient nature of the complex. The shortest distance between the redox centers is observed to be 6.2 A...
  30. pmc The crystal structure of SdsA1, an alkylsulfatase from Pseudomonas aeruginosa, defines a third class of sulfatases
    Gregor Hagelueken
    Division of Structural Biology, German Research Centre for Biotechnology, Mascheroder Weg 1, D 38124 Braunschweig, Germany
    Proc Natl Acad Sci U S A 103:7631-6. 2006
    ..The enzyme SdsA1 thus represents a previously undescribed class of sulfatases that allows P. aeruginosa to survive and thrive under otherwise bacteriocidal conditions...
  31. pmc Structure of Pseudomonas aeruginosa populations analyzed by single nucleotide polymorphism and pulsed-field gel electrophoresis genotyping
    Gracia Morales
    Departamento de Biotecnologia Microbiana, Centro Nacional de Biotecnologia, CSIC, Campus de la UAM, Cantoblanco, 28049 Madrid, Spain
    J Bacteriol 186:4228-37. 2004
    ....
  32. ncbi request reprint Proteome analysis of intraclonal diversity of two Pseudomonas aeruginosa TB clone isolates
    Catalina Arevalo-Ferro
    Department of Microbiology, Technical University of Munich, Freising, Germany
    Proteomics 4:1241-6. 2004
    ..It might be speculated that the increased expression of these proteins contributes to pathogenic competence of TB10839...