Affiliation: Fraunhofer Institute of Toxicology and Experimental Medicine
- Age-related changes in human bone marrow-derived mesenchymal stem cells: consequences for cell therapiesA Stolzing
Kroto Research Institute, North Campus, University of Sheffield, UK
Mech Ageing Dev 129:163-73. 2008..Furthermore, there is an age-related decline in overall BM MSC "fitness" which might lead to problems when using autologous aged MSC for cell-based therapies...
- Influence of murine mesenchymal stem cells on proliferation, phenotype, vitality, and cytotoxicity of murine cytokine-induced killer cells in cocultureMartin Bach
Fraunhofer Institute for Cell Therapy and Immunology, Leipzig, Germany Universitätsklinik Leipzig, Department für Innere Medizin, Neurologie und Dermatologie, Sektion Rheumatologie, Leipzig, Germany
PLoS ONE 9:e88115. 2014..Further investigations with DiD-labelled MSCs revealed that the observed disappearance of MSCs appears not to be due to differentiation processes. ..
- Effect of age and diabetes on the response of mesenchymal progenitor cells to fibrin matricesA Stolzing
Department of Cell Therapy, Fraunhofer Institute for Cell Therapy, Perlickstra β e 1, 04103 Leipzig, Germany
Int J Biomater 2011:378034. 2011..MSC were expanded on these scaffolds and analysed. MSC showed an increased self-renewal on soft surfaces. Old and diabetic cells lost the ability to react to these signals and can no longer adapt to the changed environment...
- Suspension cultures of bone-marrow-derived mesenchymal stem cells: effects of donor age and glucose levelAlexandra Stolzing
Fraunhofer Institute for Cell Therapy and Immunology IZI, Department of Cell Therapy, Leipzig, Germany
Stem Cells Dev 21:2718-23. 2012..These data indicate that rat adult bone marrow harbors a population of pluripotent Na-BM-MSCs that appear to be relatively unaffected by ageing during in vitro expansion in suspension...
- Effect of different freezing rates during cryopreservation of rat mesenchymal stem cells using combinations of hydroxyethyl starch and dimethylsulfoxideYahaira Naaldijk
Fraunhofer Institute for Cell Therapy and Immunology, Perlickstrasse 1, Leipzig, 04103, Germany
BMC Biotechnol 12:49. 2012..Post-thaw viability, phenotype and osteogenic differentiation capacity of MSCs were analysed...
- Microglia differentiation using a culture system for the expansion of mice non-adherent bone marrow stem cellsArnd Hinze
Fraunhofer Institute for Cell Therapy and Immunology, Perlickstraße 1, 04103, Leipzig, Germany
J Inflamm (Lond) 9:12. 2012..abstract:..
- The cannabinoid receptors agonist WIN55212-2 inhibits macrophageal differentiation and alters expression and phosphorylation of cell cycle control proteinsKatrin Paulsen
Division of Cellbiology, Institute of Anatomy, Faculty of Medicine, University of Zurich, Switzerland
Cell Commun Signal 9:33. 2011....
- Hydroxyethylstarch in cryopreservation - mechanisms, benefits and problemsA Stolzing
Fraunhofer Institute for Cell Therapy and Immunology, Leipzig, Germany
Transfus Apher Sci 46:137-47. 2012..However, we suggest that the most common admixture ratio of HES and DMSO in cryoprotectant solutions has been established by historical happenstance and requires further investigation and optimization...
- Angiogenic properties of aged adipose derived mesenchymal stem cells after hypoxic conditioningAnastasia Efimenko
Department of Biological and Medical Chemistry, Faculty of Fundamental Medicine, Lomonosov Moscow State University, Moscow, Russia
J Transl Med 9:10. 2011..We therefore analysed the effects of age on age markers and angiogenic properties of ADSC. Hypoxic conditioning was investigated as a form of angiogenic stimulation...
- Differentiation of mouse bone marrow derived stem cells toward microglia-like cellsArnd Hinze
Fraunhofer Institute for Cell Therapy and Immunology IZI, Perlickstrasse 1, 04103, Leipzig, Germany
BMC Cell Biol 12:35. 2011..Furthermore, we looked at the effects of FMS-like tyrosine kinase 3 ligand (Flt3L), astrocyte-conditioned medium (ACM) and GM-CSF on the differentiation to microglia-like cells...
- Fusion and regenerative therapies: is immortality really recessive?Alexandra Stolzing
Fraunhofer Institute for Cell Therapy and Immunology, Deutscher Platz 53, Leipzig, Germany
Rejuvenation Res 10:571-86. 2007..The outlook is concluded with a discussion of the next research milestones and the potential hazards of fusion therapies...
- Allogeneic non-adherent bone marrow cells facilitate hematopoietic recovery but do not lead to allogeneic engraftmentStephan Fricke
Fraunhofer Institute for Cell Therapy and Immunology IZI, Leipzig, Germany
PLoS ONE 4:e6157. 2009..Therefore, the effects of murine bone marrow derived NA-BMCs were investigated with regard to engraftment capacities in allogeneic and syngeneic stem cell transplantation using transgenic, human CD4(+), murine CD4(-/-), HLA-DR3(+) mice...
- Stressed stem cells: Temperature response in aged mesenchymal stem cellsAlexandra Stolzing
Department of Engineering Material, Centre for Tissue Engineering and Biomaterials, University of Sheffield, Sheffield, UK
Stem Cells Dev 15:478-87. 2006..The results suggest that a 'hormesis' theory of stress response can be extended to MSCs, but that cooling cultivation temperature stress produces positive effects in young cells only...
- Age-related impairment of mesenchymal progenitor cell functionAlexandra Stolzing
Centre for Biomaterials and Tissue Engineering, Department of Engineering Materials, University of Sheffield, North Campus, Sheffield, S3 7HQ, UK
Aging Cell 5:213-24. 2006....
- Watch your notch: a link between aging and stem cell fate?Alexandra Stolzing
Centre for Biomaterials and Tissue Engineering, University of Sheffield, UK
Rejuvenation Res 7:9-11. 2004
- Aging of mesenchymal stem cellsSebastian Sethe
Sheffield Institute of Biotechnological Law and Ethics, University of Sheffield, UK
Ageing Res Rev 5:91-116. 2006..Thus, mesenchymal stem cells emerge as both subject to and key mediators of organismal aging...
- Glucose-induced replicative senescence in mesenchymal stem cellsAlexandra Stolzing
Kroto Research Institute, University of Sheffield, Sheffield, United Kingdom
Rejuvenation Res 9:31-5. 2006..Reduction in glucose concentrations led to decreased apoptosis and an increased rate of MSC proliferation and increased the number and size of fibroblastic colonies in the colony-forming unit assay...
- Effect of reduced culture temperature on antioxidant defences of mesenchymal stem cellsAlexandra Stolzing
Kroto Research Institute, Department of Engineering Materials, University of Sheffield, Sheffield S3 7HQ, UK
Free Radic Biol Med 41:326-38. 2006....
- Diabetes induced changes in rat mesenchymal stem cellsAlexandra Stolzing
Kroto Research Institute, Centre for Biomaterials and Tissue Engineering, North Campus, University of Sheffield, Sheffield, UK alexandra stolzing izi fraunhofer de
Cells Tissues Organs 191:453-65. 2010..Therefore, direct effects on MSCs may be responsible for some of the orthopaedic effects associated with diabetes...
- Immunoproteasome and LMP2 polymorphism in aged and Alzheimer's disease brainsMichele Mishto
Department of Experimental Pathology, University of Bologna, 40126 Bologna, Italy
Neurobiol Aging 27:54-66. 2006....
- Chronically active: activation of microglial proteolysis in ageing and neurodegenerationAlexandra Stolzing
Centre for Biomaterials and Tissue Engineering, Sheffield University, UK
Redox Rep 10:207-13. 2005..In ageing, proteolytic effectiveness is diminished, while microglial cells are chronically activated and lose the oxidative burst ability, possibly supporting a 'vicious circle' of macrophage-induced neurodegeneration...
- Antioxidants effectively prevent oxidation-induced protein damage in OLN 93 cellsAndrea Ernst
Neuroscience Research Center, Medical Faculty Charité, Humboldt University, Berlin, Germany
Arch Biochem Biophys 421:54-60. 2004..Therefore, we concluded that the excessive oxidative damage of oligodendrocytes and their protein pool can be prevented by the usage of antioxidants...
- The consequences of acute cold exposure on protein oxidation and proteasome activity in short-tailed field voles, microtus agrestisColin Selman
Aberdeen Centre for Energy Expenditure and Obesity ACERO, Department of Zoology, Aberdeen University, UK
Free Radic Biol Med 33:259-65. 2002..This protection may be a very important factor in sustaining uncoupled respiration, which underpins nonshivering thermogenesis at cold temperatures...