Harald Schwalbe

Summary

Affiliation: Frankfurt am Main
Country: Germany

Publications

  1. ncbi request reprint Residue specific ribose and nucleobase dynamics of the cUUCGg RNA tetraloop motif by MNMR 13C relaxation
    Elke Duchardt
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University Frankfurt, Marie Curie Str 11, D 60439, Frankfurt Main, Germany
    J Biomol NMR 32:295-308. 2005
  2. ncbi request reprint Kurt Wüthrich, the ETH Zürich, and the development of NMR spectroscopy for the investigation of structure, dynamics, and folding of proteins
    Harald Schwalbe
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University Frankfurt, Marie Curie Strasse 11, Germany
    Chembiochem 4:135-42. 2003
  3. doi request reprint Double take on Piwi protein/piRNA complex structure
    Harald Schwalbe
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University, Frankfurt a M, Max von Laue Straße 7, D 60438, Frankfurt, Germany
    Structure 19:141-2. 2011
  4. ncbi request reprint Structures of RNA switches: insight into molecular recognition and tertiary structure
    Harald Schwalbe
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University Frankfurt, 60438 Frankfurt am Main, Germany
    Angew Chem Int Ed Engl 46:1212-9. 2007
  5. ncbi request reprint How much NMR data is required to determine a protein-ligand complex structure?
    Ulrich Schieborr
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe Universitat Frankfurt, Marie Curie Strasse 11, 60439 Frankfurt am Main, Germany
    Chembiochem 6:1891-8. 2005
  6. pmc Dissecting the influence of Mg2+ on 3D architecture and ligand-binding of the guanine-sensing riboswitch aptamer domain
    Janina Buck
    Institute for Organic Chemistry and Chemical Biology, Johann Wolfgang Goethe University, Max von Laue Strasse 7 and 9, 60438 Frankfurt am Main, Germany
    Nucleic Acids Res 38:4143-53. 2010
  7. doi request reprint Mechanisms for differentiation between cognate and near-cognate ligands by purine riboswitches
    Anna Wacker
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University, Frankfurt am Main, Germany
    RNA Biol 9:672-80. 2012
  8. ncbi request reprint NMR-spectroscopic characterization of phosphodiester bond cleavage catalyzed by the minimal hammerhead ribozyme
    Boris Fürtig
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University, Frankfurt, Germany
    RNA Biol 5:41-8. 2008
  9. pmc Individual basepair stability of DNA and RNA studied by NMR-detected solvent exchange
    Hannah S Steinert
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University, Frankfurt Main, Germany
    Biophys J 102:2564-74. 2012
  10. doi request reprint Conserved folding pathways of alpha-lactalbumin and lysozyme revealed by kinetic CD, fluorescence, NMR, and interrupted refolding experiments
    Kai Schlepckow
    Center for Biomolecular Magnetic Resonance, Institute for Organic Chemistry and Chemical Biology, Johann Wolfgang Goethe University, Max von Laue Str 7, D 60438 Frankfurt Main, Germany
    J Mol Biol 378:686-98. 2008

Detail Information

Publications111 found, 100 shown here

  1. ncbi request reprint Residue specific ribose and nucleobase dynamics of the cUUCGg RNA tetraloop motif by MNMR 13C relaxation
    Elke Duchardt
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University Frankfurt, Marie Curie Str 11, D 60439, Frankfurt Main, Germany
    J Biomol NMR 32:295-308. 2005
    ..Nucleobase dynamics resulting from the analysis of 13C relaxation rates were found to be in agreement with 15N relaxation data derived dynamic information (Akke et al., 1997 RNA 3, 702-709)...
  2. ncbi request reprint Kurt Wüthrich, the ETH Zürich, and the development of NMR spectroscopy for the investigation of structure, dynamics, and folding of proteins
    Harald Schwalbe
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University Frankfurt, Marie Curie Strasse 11, Germany
    Chembiochem 4:135-42. 2003
  3. doi request reprint Double take on Piwi protein/piRNA complex structure
    Harald Schwalbe
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University, Frankfurt a M, Max von Laue Straße 7, D 60438, Frankfurt, Germany
    Structure 19:141-2. 2011
    ..2011) report the structures of complexes of Piwi PAZ domains with piRNA, utilizing two major methods in structural biology, NMR spectroscopy, and X-ray crystallography, and derive very similar structures with similar resolution...
  4. ncbi request reprint Structures of RNA switches: insight into molecular recognition and tertiary structure
    Harald Schwalbe
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University Frankfurt, 60438 Frankfurt am Main, Germany
    Angew Chem Int Ed Engl 46:1212-9. 2007
    ....
  5. ncbi request reprint How much NMR data is required to determine a protein-ligand complex structure?
    Ulrich Schieborr
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe Universitat Frankfurt, Marie Curie Strasse 11, 60439 Frankfurt am Main, Germany
    Chembiochem 6:1891-8. 2005
    ....
  6. pmc Dissecting the influence of Mg2+ on 3D architecture and ligand-binding of the guanine-sensing riboswitch aptamer domain
    Janina Buck
    Institute for Organic Chemistry and Chemical Biology, Johann Wolfgang Goethe University, Max von Laue Strasse 7 and 9, 60438 Frankfurt am Main, Germany
    Nucleic Acids Res 38:4143-53. 2010
    ..We propose that reduction of conformational dynamics in remote regions of the riboswitch aptamer domain is the minimal pre-requisite to pre-organize the core region for specific ligand binding...
  7. doi request reprint Mechanisms for differentiation between cognate and near-cognate ligands by purine riboswitches
    Anna Wacker
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University, Frankfurt am Main, Germany
    RNA Biol 9:672-80. 2012
    ....
  8. ncbi request reprint NMR-spectroscopic characterization of phosphodiester bond cleavage catalyzed by the minimal hammerhead ribozyme
    Boris Fürtig
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University, Frankfurt, Germany
    RNA Biol 5:41-8. 2008
    ..Cleavage from this ensemble cannot be described by mono-exponential kinetics but follows bi-exponential kinetics. A model is described to take into account these experimental data...
  9. pmc Individual basepair stability of DNA and RNA studied by NMR-detected solvent exchange
    Hannah S Steinert
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University, Frankfurt Main, Germany
    Biophys J 102:2564-74. 2012
    ..The impact of protium-deuterium exchange of the imino hydrogen on the free energy of RNA basepair opening is investigated, and it is found that two A·U basepairs show distinct fractionation factors...
  10. doi request reprint Conserved folding pathways of alpha-lactalbumin and lysozyme revealed by kinetic CD, fluorescence, NMR, and interrupted refolding experiments
    Kai Schlepckow
    Center for Biomolecular Magnetic Resonance, Institute for Organic Chemistry and Chemical Biology, Johann Wolfgang Goethe University, Max von Laue Str 7, D 60438 Frankfurt Main, Germany
    J Mol Biol 378:686-98. 2008
    ....
  11. pmc Time-resolved NMR methods resolving ligand-induced RNA folding at atomic resolution
    Janina Buck
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University, Max von Laue Strasse 7, 60438 Frankfurt am Main, Germany
    Proc Natl Acad Sci U S A 104:15699-704. 2007
    ..Incorporation of NMR data into experimentally restrained molecular dynamics simulations provided insight into the RNA structural ensembles involved during the conformational transition...
  12. ncbi request reprint NMR backbone assignment of the N-terminal domain of human HSP90
    Doris M Jacobs
    Institut für Organische Chemie und Chemische Biologie, Zentrum für Biomolekulare Magnetische Resonanz, Johann Wolfgang Goethe Universitat Frankfurt, Max von Laue Str 7, Building N160, Room 314, D 60439, Frankfurt am Main, Germany
    J Biomol NMR 36:52. 2006
  13. ncbi request reprint NMR characterization of kinase p38 dynamics in free and ligand-bound forms
    Martin Vogtherr
    Johann Wolfgang Goethe University Frankfurt, Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Germany
    Angew Chem Int Ed Engl 45:993-7. 2006
  14. doi request reprint Mapping the landscape of RNA dynamics with NMR spectroscopy
    Jörg Rinnenthal
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University, D 60438 Frankfurt Main, Germany
    Acc Chem Res 44:1292-301. 2011
    ..These conformational transitions can be characterized with NMR spectroscopy, for any given RNA sequence, in response to external stimuli...
  15. ncbi request reprint Heterologous expression of hen egg white lysozyme and resonance assignment of tryptophan side chains in its non-native states
    Christian Schlörb
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University Frankfurt, Marie Curie Strasse 11, D 60439, Frankfurt, Germany
    J Biomol NMR 33:95-104. 2005
    ....
  16. pmc Interplay of 'induced fit' and preorganization in the ligand induced folding of the aptamer domain of the guanine binding riboswitch
    Jonas Noeske
    Institute of Organic Chemistry and Chemical Biology, Center of Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University, 60438 Frankfurt M, Germany
    Nucleic Acids Res 35:572-83. 2007
    ..The tertiary interaction motif is stable outside the riboswitch context...
  17. pmc Structure and dynamics of the deoxyguanosine-sensing riboswitch studied by NMR-spectroscopy
    Anna Wacker
    Institute for Organic Chemistry and Chemical Biology, Max von Laue Strasse 7, Frankfurt am Main, Germany
    Nucleic Acids Res 39:6802-12. 2011
    ....
  18. pmc Modulation of the stability of the Salmonella fourU-type RNA thermometer
    Jörg Rinnenthal
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University, Max von Laue Strasse 7, D 60438 Frankfurt Main, Germany
    Nucleic Acids Res 39:8258-70. 2011
    ..Binding of the two Mg(2+) ions exhibits a positive cooperativity with a Hill coefficient of 1.47. Free energy values ΔG for Mg(2+) binding determined by NMR are in agreement with data determined from CD measurements...
  19. doi request reprint MOTOR: model assisted software for NMR structure determination
    Ulrich Schieborr
    Johann Wolfgang Goethe University Frankfurt, Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Max von Laue Str 7, 60438, Frankfurt am Main, Germany
    Proteins 81:2007-22. 2013
    ....
  20. ncbi request reprint Folding and activity of cAMP-dependent protein kinase mutants
    Thomas Langer
    Johann Wolfgang Goethe University Frankfurt, Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Marie Curie Strasse 11, D 60439 Frankfurt am Main, Germany
    FEBS Lett 579:4049-54. 2005
    ..These proteins can be expressed in high yields but have reduced activity compared to the wild-type. Proper folding of all proteins was analyzed by 2D 1H, 15N-TROSY NMR experiments...
  21. ncbi request reprint Motional properties of unfolded ubiquitin: a model for a random coil protein
    Julia Wirmer
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University Frankfurt, Marie Curie Str 11, D 60439, Frankfurt, Germany
    J Biomol NMR 35:175-86. 2006
    ....
  22. ncbi request reprint Time-resolved NMR studies of RNA folding
    Boris Fürtig
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University Frankfurt, Max von Laue Str 7, Frankfurt, Germany
    Biopolymers 86:360-83. 2007
    ..Such understanding will be of importance to understand the functions of coding and non-coding RNAs in cells...
  23. doi request reprint Metal-induced folding of Diels-Alderase ribozymes studied by static and time-resolved NMR spectroscopy
    Vijayalaxmi Manoharan
    Center for Biomolecular Magnetic Resonance, Institute of Organic Chemistry and Chemical Biology, Johann Wolfgang Goethe University Frankfurt, Max von Laue Strasse 7, D 60438 Frankfurt Main, Germany
    J Am Chem Soc 131:6261-70. 2009
    ..Evidence is provided that the reactivity is modulated due to local dynamics around the catalytic pocket for the G27 MUT ribozyme...
  24. doi request reprint Direct ¹³C-detected NMR experiments for mapping and characterization of hydrogen bonds in RNA
    Boris Fürtig
    Center for Biomolecular Magnetic Resonance BMRZ, Institute of Organic Chemistry and Chemical Biology, Johann Wolfgang Goethe Universitat Frankfurt, Max von Laue Str 7, 60438, Frankfurt, Germany
    J Biomol NMR 64:207-21. 2016
    ..Such information makes the RNA secondary structure determination by NMR more accurate and helps to validate secondary structure predictions based on bioinformatics. ..
  25. pmc 13C-direct detected NMR experiments for the sequential J-based resonance assignment of RNA oligonucleotides
    Christian Richter
    Institute for Organic Chemistry and Chemical Biology, Johann Wolfgang Goethe University Frankfurt, Frankfurt M, Germany
    J Biomol NMR 47:259-69. 2010
    ..The developed methods can also be applied to large deuterated RNAs...
  26. pmc Influence of ground-state structure and Mg2+ binding on folding kinetics of the guanine-sensing riboswitch aptamer domain
    Janina Buck
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University, Max von Laue Strasse 7 and 9, 60438 Frankfurt am Main, Germany
    Nucleic Acids Res 39:9768-78. 2011
    ..We quantitatively describe the influence of distinct Mg(2+) concentrations on ligand-induced folding trajectories both by equilibrium and time-resolved NMR spectroscopy at single-residue resolution...
  27. doi request reprint RNA phosphodiester backbone dynamics of a perdeuterated cUUCGg tetraloop RNA from phosphorus-31 NMR relaxation analysis
    Jörg Rinnenthal
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University, Max von Laue Strasse 7, Frankfurt Main, Germany
    J Biomol NMR 45:143-55. 2009
    ..Nucleic Acids Res 36:1928-1940, 2008). Furthermore, the dynamics of the individual phosphate groups seem to be correlated to the 5' neighbouring nucleobases...
  28. pmc Characterization of the unfolded state of bovine alpha-lactalbumin and comparison with unfolded states of homologous proteins
    Julia Wirmer
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University Frankfurt, D 60439 Frankfurt, Germany
    Protein Sci 15:1397-407. 2006
    ..e., those between the alpha- and the beta-domain of lysozyme, may act as nucleation sites for protein folding, while this property of residual structure is replaced by the calcium-binding site between the domains in alpha-lactalbumin...
  29. doi request reprint The human Cdc37.Hsp90 complex studied by heteronuclear NMR spectroscopy
    Sridhar Sreeramulu
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University, Max von Laue Strasse 7, Frankfurt am Main D 60438, Germany
    J Biol Chem 284:3885-96. 2009
    ..NMR and mutagenesis experiments reveal Leu-205 in Cdc37 as a key residue enabling complex formation. These findings can be very useful in the development of small molecule inhibitors against cancer...
  30. doi request reprint The correlation of cathodic peak potentials of vitamin K(3) derivatives and their calculated electron affinities. The role of hydrogen bonding and conformational changes
    Hamid Reza Nasiri
    Institute of Organic Chemistry and Chemical Biology, Johann Wolfgang Goethe University Frankfurt, Max von Laue Str 7, D 60438 Frankfurt am Main, Germany
    Biochim Biophys Acta 1787:601-8. 2009
    ..This information can be used as a model to gain insight into enzyme-cofactor interactions, particularly for enzyme quinone binding modes and the electrochemical adjustment of the quinone motif...
  31. doi request reprint Structures of HIV TAR RNA-ligand complexes reveal higher binding stoichiometries
    Jan Ferner
    Institut für Organische Chemie und Chemische Biologie, Zentrum für Biomolekulare Magnetische Resonanz BMRZ, Johann Wolfgang Goethe Universität Frankfurt am Main, Max von Laue Strasse 7, 60438 Frankfurt am Main, Germany
    Chembiochem 10:1490-4. 2009
    ..The structural characterization of the RNA-ligand complex by NMR spectroscopy reveals two specific binding sites that are located at bulge residue U23 and around the pyrimidine-stretch U40-C41-U42 directly adjacent to the bulge...
  32. doi request reprint Quantitative 2D and 3D Gamma-HCP experiments for the determination of the angles alpha and zeta in the phosphodiester backbone of oligonucleotides
    Senada Nozinovic
    Institute for Organic Chemistry and Chemical Biology, Johann Wolfgang Goethe University Frankfurt, Max von Laue Strasse 7, 60438 Frankfurt am Main, Germany
    J Am Chem Soc 132:10318-29. 2010
    ..Nucleic Acids Res. 2010, 38, 683). Comparison with the X-ray structure of the cUUCGg tetraloop confirmed the high quality of the data, suggesting that the method can significantly improve the quality of RNA structure determination...
  33. doi request reprint Time-resolved NMR spectroscopy: ligand-induced refolding of riboswitches
    Janina Buck
    Institute of Organic Chemistry and Chemical Biology, Johann Wolfgang Goethe University, 60438 Frankfurt am Main, Germany
    Methods Mol Biol 540:161-71. 2009
    ..In this chapter, we discuss the application of a time-resolved NMR strategy to resolve the ligand-induced folding of the guanine-sensing riboswitch aptamer domain of the B. subtilis xpt-pbuX operon...
  34. pmc NMR and MD studies of the temperature-dependent dynamics of RNA YNMG-tetraloops
    Jan Ferner
    Institut für Organische Chemie und Chemische Biologie, Center for Biomolecular Magnetic Resonance, Frankfurt M, Germany
    Nucleic Acids Res 36:1928-40. 2008
    ..While the global fold is still preserved, this initiation of unfolding is already observed at 317 K for the uCACGg hairpin but at a significantly higher temperature for the cUUCGg hairpin...
  35. doi request reprint Functional dynamics of RNA ribozymes studied by NMR spectroscopy
    Boris Fürtig
    Institute of Organic Chemistry and Chemical Biology, Johann Wolfgang Goethe University, Frankfurt am Main, Germany
    Methods Mol Biol 848:185-99. 2012
    ....
  36. doi request reprint Three-state mechanism couples ligand and temperature sensing in riboswitches
    Anke Reining
    Center for Biomolecular Magnetic Resonance, Institute of Organic Chemistry and Chemical Biology, Johann Wolfgang Goethe Universität Frankfurt am Main, Max von Laue Strasse 7, 60438 Frankfurt am Main, Germany
    Nature 499:355-9. 2013
    ..The translational adenine-sensing riboswitch represents the first example, to our knowledge, of a temperature-compensated regulatory RNA element. ..
  37. pmc The apo-structure of the low molecular weight protein-tyrosine phosphatase A (MptpA) from Mycobacterium tuberculosis allows for better target-specific drug development
    Tanja Stehle
    Institute for Organic Chemistry and Chemical Biology, ohann Wolfgang Goethe University, Max von Laue Strasse 7, D 60438 Frankfurt am Main, Germany
    J Biol Chem 287:34569-82. 2012
    ..PtkA, the kinase complementary to MptpA, phosphorylates these two tyrosine residues in MptpA. We characterized the MptpA-PtkA interaction by NMR spectroscopy to show that both the P- and D-loop form part of the binding interface...
  38. ncbi request reprint Combined solid state and solution NMR studies of alpha,epsilon-15N labeled bovine rhodopsin
    Karla Werner
    Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe Universitat Frankfurt, Max von Laue Str 7, 60438 Frankfurt Main, Germany
    J Biomol NMR 37:303-12. 2007
    ..The results suggest that the integrated solution and solid state NMR approach presented provides highly complementary information in the study of structure and dynamics of large membrane proteins like rhodopsin...
  39. ncbi request reprint Isotope labeling of mammalian GPCRs in HEK293 cells and characterization of the C-terminus of bovine rhodopsin by high resolution liquid NMR spectroscopy
    Karla Werner
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University, Max von Laue Strasse 7, Frankfurt Main, D 60438, Germany
    J Biomol NMR 40:49-53. 2008
    ..This resulted in the assignment and detailed study of the dynamic properties of the C-terminus of rhodopsin. The rhodopsin C-terminus is immobilized until Ala333, after which it becomes unstructured...
  40. doi request reprint Theoretical study of the human bradykinin-bradykinin B2 receptor complex
    Artur Gieldon
    Johann Wolfgang Goethe Universitat, Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Frankfurt Germany
    Chembiochem 9:2487-97. 2008
    ..Additionally, we compared and discussed our putative model in the context of the structural model of the partially activated rhodopsin (Rh*) and with the known biochemical and structural data...
  41. doi request reprint Probing mechanism and transition state of RNA refolding
    Boris Fürtig
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University, Frankfurt am Main, Germany
    ACS Chem Biol 5:753-65. 2010
    ....
  42. pmc Influence of heparin mimetics on assembly of the FGF.FGFR4 signaling complex
    Krishna Saxena
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University Frankfurt, Frankfurt, Germany
    J Biol Chem 285:26628-40. 2010
    ..Notably FGF2.HM(8) exhibits pronounced positive binding cooperativity. Based on our findings we propose a refined symmetric FGF.FGFR dimerization model, which incorporates the differential ability of HM to dimerize FGFs...
  43. doi request reprint Photoresponsive Formation of an Intermolecular Minimal G-Quadruplex Motif
    Julie Thevarpadam
    Goethe University Frankfurt, Institute for Organic Chemistry and Chemical Biology, Buchmann Institute for Molecular Life Sciences, Max von Laue Strasse 9, 60438, Frankfurt, Germany
    Angew Chem Int Ed Engl 55:2738-42. 2016
    ..However, only the para-para-substituted azobenzene derivative enables photoswitching between a nonpolymorphic, stacked, tetramolecular G-quadruplex and an unstructured state after E-Z isomerization...
  44. ncbi request reprint NMR spectroscopy of RNA
    Boris Fürtig
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University, Marie Curie Strasse 11, 60439 Frankfurt am Main, Germany
    Chembiochem 4:936-62. 2003
    ....
  45. pmc An intermolecular base triple as the basis of ligand specificity and affinity in the guanine- and adenine-sensing riboswitch RNAs
    Jonas Noeske
    Institute of Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe Universitat, Marie Curie Strasse 11, D 60439 Frankfurt am Main, Germany
    Proc Natl Acad Sci U S A 102:1372-7. 2005
    ....
  46. ncbi request reprint Quantitative gamma-HCNCH: determination of the glycosidic torsion angle chi in RNA oligonucleotides from the analysis of CH dipolar cross-correlated relaxation by solution NMR spectroscopy
    Jörg Rinnenthal
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University, Max von Laue Strasse 7, 60438 Frankfurt Main, Germany
    J Biomol NMR 39:17-29. 2007
    ....
  47. pmc Direct observation of the temperature-induced melting process of the Salmonella fourU RNA thermometer at base-pair resolution
    Jörg Rinnenthal
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University, Max von Laue Strasse 7, D 60438 Frankfurt Main, Germany
    Nucleic Acids Res 38:3834-47. 2010
    ..Furthermore, remote effects of the A8C mutation affecting the stability of nucleobase G14 could be identified. According to our analysis we deduce that this effect is most probably transduced via the hydration shell of the RNA...
  48. doi request reprint Design, synthesis, and biological testing of novel naphthoquinones as substrate-based inhibitors of the quinol/fumarate reductase from Wolinella succinogenes
    Hamid Reza Nasiri
    Institute of Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University Frankfurt, Max von Laue Straße 7, D 60438 Frankfurt am Main, Germany
    J Med Chem 56:9530-41. 2013
    ..Compounds 4a and 4b showed IC50 values in low micromolar range in the primary assay and no activity in the counter DT-diaphorase assay. ..
  49. pmc High-resolution NMR structure of an RNA model system: the 14-mer cUUCGg tetraloop hairpin RNA
    Senada Nozinovic
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University Frankfurt, Max von Laue Strasse 7, 60438 Frankfurt am Main, Germany
    Nucleic Acids Res 38:683-94. 2010
    ..Here, we discuss the impact of various NMR restraints on structure quality and discuss in detail the dynamics of this system as previously determined...
  50. doi request reprint Characterization of the simultaneous decay kinetics of metarhodopsin states II and III in rhodopsin by solution-state NMR spectroscopy
    Jochen Stehle
    Institute for Organic Chemistry and Chemical Biology, Center of Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University Frankfurt, Max von Laue Strasse 7, 60438 Frankfurt am Main Germany
    Angew Chem Int Ed Engl 53:2078-84. 2014
    ..Transient formation of the meta III state was confirmed by flash photolysis experiments. From analysis of the site-resolved kinetic data we propose the involvement of the E2 -loop in the formation of the meta III state...
  51. ncbi request reprint Photo-CIDNP reveals differences in compaction of non-native states of lysozyme
    Christian Schlörb
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University, Marie Curie Strasse 11, D 60439 Frankfurt M, Germany
    J Am Chem Soc 128:1802-3. 2006
    ..These data can be interpreted in the context of the hydrophobic clustering around the tryptophan residues and is supported by the application of this method to the cluster breaking W62G mutant of lysozyme...
  52. doi request reprint Characterization of the ground state dynamics of proteorhodopsin by NMR and optical spectroscopies
    Jochen Stehle
    Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt, Germany
    J Biomol NMR 54:401-13. 2012
    ....
  53. doi request reprint Disentangling the coil: modulation of conformational and dynamic properties by site-directed mutation in the non-native state of hen egg white lysozyme
    Friederike Sziegat
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance BMRZ, Johann Wolfgang Goethe University, Frankfurt a M, Max von Laue Straße 7, D 60438 Frankfurt, Germany
    Biochemistry 51:3361-72. 2012
    ....
  54. pmc Isotope labeling in insect cells
    Krishna Saxena
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University Frankfurt, Frankfurt am Main, Germany
    Methods Mol Biol 831:37-54. 2012
    ..Here, we provide protocols that have proven successful in such endeavors...
  55. ncbi request reprint Tripeptides from synthetic amino acids block the Tat-TAR association and slow down HIV spread in cell cultures
    Verena Ludwig
    Institute of Organic Chemistry and Chemical Biology, Johann Wolfgang Goethe Universitat Frankfurt, Max von Laue Strasse 7, 60438 Frankfurt am Main, Germany
    Chembiochem 8:1850-6. 2007
    ..Peptide 7, which contains a 2-pyrimidinyl-alkyl chain, also inhibited the spread of HIV-1 in cell cultures. NMR studies of 7 bound to HIV-2-TAR gave evidence for contacts in the bulge region...
  56. ncbi request reprint Conformational dynamics of bistable RNAs studied by time-resolved NMR spectroscopy
    Boris Fürtig
    Johann Wolfgang Goethe University, Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Max von Laue Str 7, 60438 Frankfurt, Germany
    J Am Chem Soc 129:16222-9. 2007
    ..Comparison of these results with bistable systems previously investigated leads to the prediction of activation enthalpies (DeltaH++) associated with global structural transitions in RNA...
  57. doi request reprint HNHC: a triple resonance experiment for correlating the H2, N1(N3) and C2 resonances in adenine nucleobases of 13C-, 15N-labeled RNA oligonucleotides
    Jörg Rinnenthal
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University, Max von Laue Strasse 7, 60438 Frankfurt Main, Germany
    J Biomol NMR 44:101-5. 2009
    ..in J Biomol NMR 20:173-176 2001) is provided...
  58. doi request reprint Optimizing the kinetics and thermodynamics of DNA i-motif folding
    Anna Lena Lieblein
    Institute for Organic Chemistry and Chemical Biology, Center of Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University Frankfurt Main, Max von Laue Straße 7, 60438 Frankfurt Germany
    Chembiochem 14:1226-30. 2013
    ..The stabilities of the i-motif structures were determined by CD melting, and structure and kinetics of folding were studied by static and time-resolved NMR experiments. ..
  59. ncbi request reprint Determination of the glycosidic bond angle chi in RNA from cross-correlated relaxation of CH dipolar coupling and N chemical shift anisotropy
    Elke Duchardt
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University Frankfurt, Marie Curie Strasse 11, D 60439 Frankfurt Main, Germany
    J Am Chem Soc 126:1962-70. 2004
    ..As shown for the 30-mer RNA, the derived torsion angle information can be incorporated as additional restraint, improving RNA structure calculations...
  60. ncbi request reprint Phosphate-group recognition by the aptamer domain of the thiamine pyrophosphate sensing riboswitch
    Jonas Noeske
    Institute of Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University, 60438 Frankfurt am Main, Germany
    Chembiochem 7:1451-6. 2006
    ....
  61. pmc L11 domain rearrangement upon binding to RNA and thiostrepton studied by NMR spectroscopy
    Hendrik R A Jonker
    Johann Wolfgang Goethe University, Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Max von Laue Strasse 7, 60438 Frankfurt am Main, Germany
    Nucleic Acids Res 35:441-54. 2007
    ..The model is consistent with earlier findings and provides an explanation for the role of L11(ntd) in elongation factor binding...
  62. ncbi request reprint Structure and dynamics of the homologous series of alanine peptides: a joint molecular dynamics/NMR study
    Jürgen Graf
    Institute of Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University Frankfurt, Max von Laue Strasse 7, D 60438 Frankfurt Main, Germany
    J Am Chem Soc 129:1179-89. 2007
    ....
  63. doi request reprint ¹H, ¹³C and ¹⁵N assignment of D2 domain of human fibroblast growth factor receptor 4
    Bettina Elshorst
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University Frankfurt, Max von Laue Str 7, 60438 Frankfurt, Germany
    Biomol NMR Assign 7:179-82. 2013
    ..We report the sequential backbone and side chain resonance assignment of the D2 domain of human FGFR4. ..
  64. ncbi request reprint Structure and dynamics of an RNA tetraloop: a joint molecular dynamics and NMR study
    Jessica Koplin
    Institute of Physical and Theoretical Chemistry, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University, Frankfurt, Marie Curie Str 11, D 60439 Frankfurt Main, Germany
    Structure 13:1255-67. 2005
    ..No correlation between the motion of the sugar rings and the stacking dynamics of the loop bases is found...
  65. doi request reprint Modulation of structure and dynamics by disulfide bond formation in unfolded states
    Robert Silvers
    Institute for Organic Chemistry and Chemical Biology, Center of Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max von Laue Strasse 7, 60438 Frankfurt am Main, Germany
    J Am Chem Soc 134:6846-54. 2012
    ....
  66. doi request reprint NMR spectroscopic characterization of the adenine-dependent hairpin ribozyme
    Janina Buck
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University, 60438 Frankfurt am Main Germany
    Chembiochem 10:2100-10. 2009
    ..We show that conformational dynamics, but not the chemistry, constitute the rate-limiting step in catalysis of the adenine-dependent hairpin ribozyme...
  67. ncbi request reprint New NMR experiments for RNA nucleobase resonance assignment and chemical shift analysis of an RNA UUCG tetraloop
    Boris Fürtig
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University, Marie Curie Strasse 11, D 60439 Frankfurt Main, Germany
    J Biomol NMR 28:69-79. 2004
  68. ncbi request reprint Domain reorientation and induced fit upon RNA binding: solution structure and dynamics of ribosomal protein L11 from Thermotoga maritima
    Sergey Ilin
    Institute of Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe Universitat, Marie Curie Strasse 11, 60439 Frankfurt am Main, Germany
    Chembiochem 6:1611-8. 2005
    ..The domain orientation in free L11 shows limited similarity to that of ribosome-bound L11 in complex with elongation factor, EF-G...
  69. doi request reprint The unfolded state of the murine prion protein and properties of single-point mutants related to human prion diseases
    Christian Gerum
    Goethe University, Institute of Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Max von Laue Strabetae 7, D 60438 Frankfurt Main, Germany
    J Mol Biol 401:7-12. 2010
    ..These differences in aggregation behaviour are not correlated with the thermal stability of the mutants, indicating different mechanisms promoting the conformational conversion process...
  70. doi request reprint NMR studies of HAR1 RNA secondary structures reveal conformational dynamics in the human RNA
    Melanie Ziegeler
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max von Laue Strasse 7, 60438 Frankfurt am Main, Germany
    Chembiochem 13:2100-12. 2012
    ..h37 RNA folds into a slightly more stable and rigid structure than c37 RNA. Both NMR structures show structural heterogeneity of the residues corresponding to the GAA loop...
  71. doi request reprint Recombinant expression and purification of human TATA binding protein using a chimeric fusion
    Robert Silvers
    Institute for Organic Chemistry and Chemical Biology, Center of Biomolecular Magnetic Resonance, Goethe University Frankfurt, Frankfurt am Main, Germany
    Protein Expr Purif 85:142-7. 2012
    ..Here, we present a novel approach for high yield expression and purification of human TBP core (hTBPc) protein. Protein fold and activity are verified by nuclear magnetic resonance (NMR) spectroscopy and microscale thermophoresis (MST)...
  72. doi request reprint NMR spectroscopic investigations of the activated p38α mitogen-activated protein kinase
    Gerd Nielsen
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University, Max von Laue Strasse 7, 60438 Frankfurt am Main, Germany
    Chembiochem 12:2599-607. 2011
    ..Deuterium-exchange experiments additionally revealed differences in exchange behavior for two residues in an alanine-rich helix-loop motif that becomes more flexible upon binding of an ATP analogue and a substrate peptide...
  73. ncbi request reprint 1H, 13C and 15N backbone resonance assignment of the integrin alpha2 I-domain
    Bettina Elshorst
    Johann Wolfgang Goethe University Frankfurt, Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Marie Curie Strasse 11, D 60439 Frankfurt am Main, Germany
    J Biomol NMR 27:191-2. 2003
  74. ncbi request reprint NMR backbone assignment of a protein kinase catalytic domain by a combination of several approaches: application to the catalytic subunit of cAMP-dependent protein kinase
    Thomas Langer
    Johann Wolfgang Goethe Universitat Frankfurt, Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Marie Curie Strasse 11, 60439 Frankfurt am Main, Germany
    Chembiochem 5:1508-16. 2004
    ..Furthermore, structural conformational changes were observed by comparison of backbone amide shifts obtained by 2D (1)H,(15)N TROSY of an inactive Thr197Ala mutant with the wild-type enzyme...
  75. ncbi request reprint Characterisation of disulfide-bond dynamics in non-native states of lysozyme and its disulfide deletion mutants by NMR
    Emily S Collins
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University, Marie Curie Strasse 11, 60439 Frankfurt am Main, Germany
    Chembiochem 6:1619-27. 2005
    ..However, the interactions between hydrophobic clusters within and across the domains remains intact...
  76. ncbi request reprint Biomolecular NMR: a chaperone to drug discovery
    Marco Betz
    Johann Wolfgang Goethe University Frankfurt, Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Marie Curie Str 11, D 60439 Frankfurt am Main, Germany
    Curr Opin Chem Biol 10:219-25. 2006
    ..An overview of different steps in recent drug discovery is presented to illuminate the links with the most recent advances in NMR methodology...
  77. ncbi request reprint Theoretical framework for NMR residual dipolar couplings in unfolded proteins
    O I Obolensky
    Frankfurt Institute for Advanced Studies, Max von Laue Str 1, 60438 Frankfurt am Main, Germany
    J Biomol NMR 39:1-16. 2007
    ..Various improvements and generalizations are possible within the suggested approach...
  78. doi request reprint The high resolution NMR structure of parvulustat (Z-2685) from Streptomyces parvulus FH-1641: comparison with tendamistat from Streptomyces tendae 4158
    Stephan Rehm
    Institute of Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University, Max von Laue Strasse 7, Frankfurt am Main, Germany
    Chembiochem 10:119-27. 2009
    ..These structural and dynamical differences indicate that for parvulustat an induced-fit mechanism for binding to alpha-amylase might take place, since the structure of tendamistat does not change upon binding to alpha-amylase...
  79. ncbi request reprint Structure induction of the T-cell receptor zeta-chain upon lipid binding investigated by NMR spectroscopy
    Elke Duchardt
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University Frankfurt, 60439 Frankfurt, Germany
    Chembiochem 8:820-7. 2007
    ..Regions around the ITAM2 and ITAM3 sequences are involved in a highly dynamic binding of the free zeta-chain to a detergent micelle formed by the acidic lipid LMPG...
  80. pmc Starting structure dependence of NMR order parameters derived from MD simulations: implications for judging force-field quality
    Alrun N Koller
    Department of Biological Sciences, Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Goethe University, Frankfurt am Main, Germany
    Biophys J 95:L04-6. 2008
    ..We show that adequately sampling flexible regions ( approximately 100 ns) and only calculating S(2) parameters averaged over short time windows proved necessary to obtain consistent results irrespective of the starting structure...
  81. doi request reprint The HN(COCA)HAHB NMR experiment for the stereospecific assignment of Hbeta-protons in non-native states of proteins
    Martin J Hähnke
    Center for Biomolecular Magnetic Resonance, Institute of Organic Chemistry and Chemical Biology, Johann Wolfgang Goethe University Frankfurt, Max von Laue Strasse 7, D 60438 Frankfurt Main, Germany
    J Am Chem Soc 132:918-9. 2010
    ....
  82. pmc Engineering encodable lanthanide-binding tags into loop regions of proteins
    Katja Barthelmes
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University of Frankfurt, Max von Laue Strasse 7, 60438 Frankfurt, Germany
    J Am Chem Soc 133:808-19. 2011
    ..The current data provide support that encodable LBTs serve as versatile biophysical tags when inserted into loop regions of proteins of known structure or predicted via homology modeling...
  83. ncbi request reprint Triple resonance experiments for the simultaneous correlation of H6/H5 and exchangeable protons of pyrimidine nucleotides in 13C,15N-labeled RNA applicable to larger RNA molecules
    Jens Wöhnert
    Institut für Organische Chemie und Chemische Biologie, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe Universitat Frankfurt, Marie Curie Str 11, 60439 Frankfurt M, Germany
    J Biomol NMR 26:79-83. 2003
    ..9 mM at temperatures of 10 degrees C and 25 degrees C. This indicates the general applicability of the experiments and the possibility to obtain correlations for imino resonances in non-canonical regions of the target RNA...
  84. ncbi request reprint 1H, 13C and 15N backbone resonance assignment of the VASP EVH1 domain
    Martin Vogtherr
    Institut für Organische Chemie und Chemische Biologie, Zentrum für Biomolekulare Magnetische Resonanz, Johann Wolfgang Goethe Universitat, Marie Curie Strasse 11, D 60439 Frankfurt am Main, Germany
    J Biomol NMR 27:189-90. 2003
  85. ncbi request reprint 2-Hydroxy-3-(3-oxobutyl)naphthalene-1,4-dione
    Hamid Reza Nasiri
    Institute of Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, J W Goethe Universität Frankfurt, Max von Laue Strasse 7, 60438 Frankfurt Main, Germany
    Acta Crystallogr C 62:o671-3. 2006
    ..O hydrogen bond between the hydroxy group and the adjacent carbonyl O atom. The crystal structure is stabilized by O-H...O hydrogen bonds connecting the molecules into zigzag chains running along the b axis...
  86. ncbi request reprint Modulation of compactness and long-range interactions of unfolded lysozyme by single point mutations
    Julia Wirmer
    Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe University Frankfurt, Marie Curie Strasse 11, 60439 Frankfurt, Germany
    Angew Chem Int Ed Engl 43:5780-5. 2004
  87. ncbi request reprint Protein alignment by a coexpressed lanthanide-binding tag for the measurement of residual dipolar couplings
    Jens Wöhnert
    Institut für Organische Chemie und Chemische Biologie, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe Universitat Frankfurt, Marie Curie Strasse 11, 60439 Frankfurt M, Germany
    J Am Chem Soc 125:13338-9. 2003
    ..6 to 6.1 Hz for Dy3+, while an opposite alignment tensor is observed for Tm3+ (4.5 to -2.9 Hz) at 800 MHz. Experimental RDCs are in excellent agreement with those predicted on the basis of the X-ray structure of the protein...
  88. doi request reprint Evaluation of parameters critical for observing nucleic acids inside living Xenopus laevis oocytes by in-cell NMR spectroscopy
    Robert Hänsel
    Institute of Biophysical Chemistry, Goethe University, Max von Laue Str 9, 60438 Frankfurt am Main, Germany
    J Am Chem Soc 131:15761-8. 2009
    ..Finally, we show that the imino region of the NMR spectrum is devoid of any oocyte background signals enabling the detection even of isotopically nonlabeled molecules...
  89. ncbi request reprint Long-range interactions within a nonnative protein
    Judith Klein-Seetharaman
    Massachusetts Institute of Technology, Department of Chemistry, Francis Bitter Magnet Laboratory, 170 Albany Street, Cambridge, MA 02139, USA
    Science 295:1719-22. 2002
    ..Thus, nativelike structure in the denatured protein is stabilized by the involvement of Trp62 in nonnative and long-range interactions...
  90. ncbi request reprint Angular dependence of 1J(Ni,Calphai) and 2J(Ni,Calpha(i-1)) coupling constants measured in J-modulated HSQCs
    Julia Wirmer
    Department of Chemistry and MIT Harvard Center for Magnetic Resonance at the Francis Bitter Magnet Laboratory, Massachusetts Institute of Technology, Cambridge 02139, USA
    J Biomol NMR 23:47-55. 2002
    ..Furthermore it was shown that the observed coupling constants were in good agreement with predicted coupling constants using a simple model for the random coil...
  91. ncbi request reprint Dissecting G-protein-coupled receptors: structure, function, and ligand interaction
    Harald Schwalbe
    Chembiochem 3:915-9. 2002
  92. pmc Differential dynamics in the G protein-coupled receptor rhodopsin revealed by solution NMR
    Judith Klein-Seetharaman
    Department of Pharmacology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    Proc Natl Acad Sci U S A 101:3409-13. 2004
    ..In contrast, indole side-chain (15)N groups of tryptophans showed well resolved, equally intense NMR signals, suggesting restriction to a single specific conformation...
  93. ncbi request reprint Kinetics of photoinduced RNA refolding by real-time NMR spectroscopy
    Philipp Wenter
    Ecole Polytechnique Federale de Lausanne, Laboratory of Nucleic Acid Chemistry, EPFL BCH, 1015 Lausanne, Switzerland
    Angew Chem Int Ed Engl 44:2600-3. 2005
  94. ncbi request reprint NMR backbone assignment of the mitogen-activated protein (MAP) kinase p38
    Martin Vogtherr
    J Biomol NMR 32:175. 2005
  95. ncbi request reprint 1H, 13C and 15N backbone resonance assignment of the Hsp90 binding domain of human Cdc37
    Sridhar Sreeramulu
    J Biomol NMR 32:262. 2005
  96. ncbi request reprint Backbone NMR assignment of the C-terminal haemopexin-like domain (HPLD) of human matrix metalloproteinase MMP-13
    Doris M Jacobs
    J Biomol NMR 32:337. 2005
  97. ncbi request reprint Backbone NMR assignment of the human E2 ubiquitin conjugating enzyme UbcH5alpha (F72K,F82S) double mutant
    Krishna Saxena
    J Biomol NMR 32:338. 2005
  98. ncbi request reprint Glutamic acid-rich proteins of rod photoreceptors are natively unfolded
    Renu Batra-Safferling
    Institut für Biologische Informationsverarbeitung 1, Forschungszentrum Julich, Germany
    J Biol Chem 281:1449-60. 2006
    ..GARP proteins could then provide the environment needed for protein interactions in the rim region of discs...
  99. pmc Experimental support for the "E pathway hypothesis" of coupled transmembrane e- and H+ transfer in dihemic quinol:fumarate reductase
    C Roy D Lancaster
    Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max von Laue Strasse 3, D 60438 Frankfurt am Main, Germany
    Proc Natl Acad Sci U S A 102:18860-5. 2005
    ..The results provide experimental evidence for the validity of the E pathway hypothesis and for a crucial functional role of Glu-C180...
  100. ncbi request reprint A caged uridine for the selective preparation of an RNA fold and determination of its refolding kinetics by real-time NMR
    Philipp Wenter
    Laboratory of Nucleic Acid Chemistry, Ecole Polytechnique Federale de Lausanne EPFL, 1015 Lausanne, Switzerland
    Chembiochem 7:417-20. 2006
  101. ncbi request reprint NMR analysis of a Tau phosphorylation pattern
    Isabelle Landrieu
    CNRS UMR 8525, Institut Pasteur de Lille, 59019 Lille Cedex, France
    J Am Chem Soc 128:3575-83. 2006
    ..We finally demonstrate that the NMR approach can equally be used to evaluate potential kinase inhibitors in a straightforward manner...