W Preiser

Summary

Affiliation: Frankfurt am Main
Country: Germany

Publications

  1. ncbi request reprint Acute retinal necrosis six years after herpes simplex encephalitis: an elusive immune deficit suggested by insufficient test sensitivity
    W Preiser
    Institute for Medical Virology, Johann Wolfgang Goethe University Hospital, Frankfurt am Main, Germany
    J Med Virol 73:250-5. 2004
  2. ncbi request reprint Evaluation of diagnostic methods for the detection of cytomegalovirus in recipients of allogeneic stem cell transplants
    W Preiser
    Institute for Medical Virology, Johann Wolfgang Goethe University Hospital, Frankfurt am Main 60054, Germany
    J Clin Virol 20:59-70. 2001
  3. ncbi request reprint Development and clinical application of a fully controlled quantitative PCR assay for cell-free cytomegalovirus in human plasma
    Wolfgang Preiser
    Department of Virology, Royal Free and University College School of Medicine and University College London Hospitals NHS Trust UCLH, Windeyer Building, 46 Cleveland Street, London W1P 6DB, UK
    J Clin Virol 26:49-59. 2003
  4. ncbi request reprint [New methods for the diagnosis of cytomegalovirus]
    W Preiser
    Institut fur Medizinische Virologie, Klinikum der Johann Wolfgang Goethe Universität Frankfurt am Main, Frankfurt am Main
    Dtsch Med Wochenschr 129:1509-12. 2004
  5. ncbi request reprint Primary cytomegalovirus infection in an outpatient setting--laboratory markers and clinical aspects
    G Just-Nübling
    Dept of Infectiology, Medical Clinic III, J W Goethe University Hospital, Theodor Stern Kai 7, D 60590, Frankfurt am Main, Germany
    Infection 31:318-23. 2003
  6. ncbi request reprint Enterovirus infections in Germany: comparative evaluation of different laboratory diagnostic methods
    S Buxbaum
    Institute for Medical Virology, JW Goethe University Frankfurt am Main, Germany
    Infection 29:138-42. 2001
  7. ncbi request reprint Infection of cultured intestinal epithelial cells with severe acute respiratory syndrome coronavirus
    J Cinatl
    Institute for Medical Virology, Johann Wolfgang Goethe University Hospital, Paul Ehrlich Str 40, 60596, Frankfurt am Main, Germany
    Cell Mol Life Sci 61:2100-12. 2004
  8. ncbi request reprint Kaposi's sarcoma-associated herpesvirus seroprevalence in selected german patients: evaluation by different test systems
    W Preiser
    Institute for Medical Virology, Johann Wolfgang Goethe University Hospital, Frankfurt am Main, Germany
    Med Microbiol Immunol 190:121-7. 2001
  9. ncbi request reprint Laboratory diagnosis of influenza--virology or serology?
    R Allwinn
    Institute for Medical Virology, Johann Wolfgang Goethe University, Paul Ehrlich Str 40, 60596 Frankfurt am Main, Germany
    Med Microbiol Immunol 191:157-60. 2002
  10. ncbi request reprint Performance characteristics of an automated PCR assay for the quantification of cytomegalovirus DNA in plasma
    W Preiser
    Institute for Medical Virology, Johann Wolfgang Goethe University Hospital, Paul Ehrlich Strasse 40, D 60596, Frankfurt am Main, Germany
    J Virol Methods 101:149-57. 2002

Collaborators

Detail Information

Publications47

  1. ncbi request reprint Acute retinal necrosis six years after herpes simplex encephalitis: an elusive immune deficit suggested by insufficient test sensitivity
    W Preiser
    Institute for Medical Virology, Johann Wolfgang Goethe University Hospital, Frankfurt am Main, Germany
    J Med Virol 73:250-5. 2004
    ..Unless this potential problem is recognised, serious consequences might possibly result. We therefore urge virologists and clinicians to exercise great care if highly specific antibody assays based on recombinant proteins are employed...
  2. ncbi request reprint Evaluation of diagnostic methods for the detection of cytomegalovirus in recipients of allogeneic stem cell transplants
    W Preiser
    Institute for Medical Virology, Johann Wolfgang Goethe University Hospital, Frankfurt am Main 60054, Germany
    J Clin Virol 20:59-70. 2001
    ..Although several diagnostic methods are available for the surveillance of patients at risk of human cytomegalovirus (CMV) infection and disease, little data is available on their comparative performances in the diagnostic setting...
  3. ncbi request reprint Development and clinical application of a fully controlled quantitative PCR assay for cell-free cytomegalovirus in human plasma
    Wolfgang Preiser
    Department of Virology, Royal Free and University College School of Medicine and University College London Hospitals NHS Trust UCLH, Windeyer Building, 46 Cleveland Street, London W1P 6DB, UK
    J Clin Virol 26:49-59. 2003
    ..The quantification of HCMV genome is clinically useful for the diagnosis of HCMV disease, for the virological surveillance of high-risk patients and for monitoring antiviral therapy...
  4. ncbi request reprint [New methods for the diagnosis of cytomegalovirus]
    W Preiser
    Institut fur Medizinische Virologie, Klinikum der Johann Wolfgang Goethe Universität Frankfurt am Main, Frankfurt am Main
    Dtsch Med Wochenschr 129:1509-12. 2004
    ..This review article deals with general and fundamental aspects...
  5. ncbi request reprint Primary cytomegalovirus infection in an outpatient setting--laboratory markers and clinical aspects
    G Just-Nübling
    Dept of Infectiology, Medical Clinic III, J W Goethe University Hospital, Theodor Stern Kai 7, D 60590, Frankfurt am Main, Germany
    Infection 31:318-23. 2003
    ..Occasionally, primary cytomegalovirus (CMV) infection may give rise to more or less severe clinical illness in immunocompetent adults. We retrospectively analyzed cases of acute CMV infection in medical outpatients...
  6. ncbi request reprint Enterovirus infections in Germany: comparative evaluation of different laboratory diagnostic methods
    S Buxbaum
    Institute for Medical Virology, JW Goethe University Frankfurt am Main, Germany
    Infection 29:138-42. 2001
    ..Serologically, a significant rise in antibody titer may be detected and different enteroviral types can be differentiated using the neutralization assay...
  7. ncbi request reprint Infection of cultured intestinal epithelial cells with severe acute respiratory syndrome coronavirus
    J Cinatl
    Institute for Medical Virology, Johann Wolfgang Goethe University Hospital, Paul Ehrlich Str 40, 60596, Frankfurt am Main, Germany
    Cell Mol Life Sci 61:2100-12. 2004
    ..In both cell lines, SARS-CoV infection deregulated expression of cellular genes which may be important for the intestinal pathogenesis of SARS...
  8. ncbi request reprint Kaposi's sarcoma-associated herpesvirus seroprevalence in selected german patients: evaluation by different test systems
    W Preiser
    Institute for Medical Virology, Johann Wolfgang Goethe University Hospital, Frankfurt am Main, Germany
    Med Microbiol Immunol 190:121-7. 2001
    ..Despite the lack of a "gold standard" for KSHV antibody detection, the fact that the results obtained overall agreed rather well indicates their suitability for conducting seroepidemiological studies...
  9. ncbi request reprint Laboratory diagnosis of influenza--virology or serology?
    R Allwinn
    Institute for Medical Virology, Johann Wolfgang Goethe University, Paul Ehrlich Str 40, 60596 Frankfurt am Main, Germany
    Med Microbiol Immunol 191:157-60. 2002
    ..In comparison, virus detection is clearly superior to antibody determination for diagnosis of influenza virus infections. However, antibody testing may be useful as a complementary tool to confirm the diagnosis retrospectively...
  10. ncbi request reprint Performance characteristics of an automated PCR assay for the quantification of cytomegalovirus DNA in plasma
    W Preiser
    Institute for Medical Virology, Johann Wolfgang Goethe University Hospital, Paul Ehrlich Strasse 40, D 60596, Frankfurt am Main, Germany
    J Virol Methods 101:149-57. 2002
    ..992. On balance, the test has acceptable within-run and between-run reproducibility. It therefore allows the comparison of results obtained at different time-points as well as in different laboratories, e.g. in multi-centre studies...
  11. ncbi request reprint The role of viral load determination for the management of human immunodeficiency virus, hepatitis B virus and hepatitis C virus infection
    A Berger
    Institut fur Medizinische Virologie, Zentrum der Hygiene, Universitatskliniken, Paul Ehrlich Str 40, D 60596 Frankfurt am Main, Germany
    J Clin Virol 20:23-30. 2001
    ..However, viral load may predict the outcome of antiviral therapy. Genetic diversity is another challenge for HCV RNA quantification...
  12. ncbi request reprint Cytomegalovirus infection decreases expression of thrombospondin-1 and -2 in cultured human retinal glial cells: effects of antiviral agents
    J Cinatl
    Institut fur Medizinische Virologie, Zentrum der Hygiene, Klinikum der Johann Wolfgang Goethe Universitat, D 60596 Frankfurt am Main, Germany
    J Infect Dis 182:643-51. 2000
    ..The results showed that HCMV-induced down-modulation of TSP-1 and TSP-2 in retinal glial cells is prevented by inhibition of HCMV IE expression. These findings may be relevant to pathogenesis and treatment of HCMV retinitis...
  13. ncbi request reprint Travel-acquired dengue infection: clinical spectrum and diagnostic aspects
    C Stephan
    Clinic for Internal Medicine, Dept of Infectious Disease, Frankfurt am Main, Germany
    Infection 30:225-8. 2002
    ..Only few studies focus on clinical manifestations and serological findings in primarily nonimmune individuals...
  14. ncbi request reprint Seroprevalence of herpes simplex virus types 1 and type 2 in the Frankfurt am Main area, Germany
    H F Rabenau
    Institute for Medical Virology, Johann Wolfgang Goethe University Hospital, Frankfurt am Main, Germany
    Med Microbiol Immunol 190:153-60. 2002
    ..The same is true when investigating HSV-1 seroprevalence rates in HSV-2-seropositive or -negative individuals, retrospectively...
  15. ncbi request reprint Polyomavirus viruria in bone marrow transplant recipients: lack of correlation with clinical symptoms
    H F Rabenau
    Institute for Medical Virology, Johann Wolfgang Goethe University Clinic, Frankfurt am Main, Germany
    Infection 30:91-3. 2002
    ..Human polyomavirus (HPV) infection is controversially discussed as a factor influencing the outcome of bone marrow transplantation (BMT)...
  16. ncbi request reprint Laboratory diagnosis of norovirus: which method is the best?
    H F Rabenau
    Institute of Medical Virology, Johann Wolfgang Goethe University, Frankfurt, Germany
    Intervirology 46:232-8. 2003
    ..4%). All three methods tested (TEM, ELISA and PCR) are useful for epidemiological investigations in gastroenteritis outbreaks; however, to maximize diagnostic validity for individual cases, at least two of the methods should be combined...
  17. ncbi request reprint Cross-reactivity in flavivirus serology: new implications of an old finding?
    R Allwinn
    Institute for Medical Virology, Johann Wolfgang Goethe University Hospital, Frankfurt am Main, Germany
    Med Microbiol Immunol 190:199-202. 2002
    ....
  18. ncbi request reprint Stability and inactivation of SARS coronavirus
    H F Rabenau
    Institute for Medical Virology, Frankfurt am Main University Hospital Medical School, Germany
    Med Microbiol Immunol 194:1-6. 2005
    ..Nevertheless, the agents tenacity is considerably higher than that of HCoV-229E, and should SARS re-emerge, increased efforts need to be devoted to questions of environmental hygiene...
  19. ncbi request reprint Viral zoonoses - a threat under control?
    B Ludwig
    Institute for Medical Virology, Johann Wolfgang Goethe University, Frankfurt am Main, Germany
    Intervirology 46:71-8. 2003
    ..In this review, we present the important examples of zoonotic viruses and discuss the factors playing a key role in the emergence and resurgence of these diseases...
  20. ncbi request reprint Variety of interpretation systems for human immunodeficiency virus type 1 genotyping: confirmatory information or additional confusion?
    M Sturmer
    Institute for Medical Virology, Johann Wolfgang Goethe University Hospital Frankfurt am Main, Germany
    Curr Drug Targets Infect Disord 3:373-82. 2003
    ..Whilst not recommending one particular system, the authors believe that the correlation of genotypic with clinical data is probably the best way to develop an optimal algorithm...
  21. ncbi request reprint Severe acute respiratory syndrome (SARS)--paradigm of an emerging viral infection
    A Berger
    Institute for Medical Virology, Johann Wolfgang Goethe University Hospital, Paul Ehrlich Street 40, D 60596 Frankfurt a M, Germany
    J Clin Virol 29:13-22. 2004
    ....
  22. ncbi request reprint No apparent effect of cidofovir in epidermodysplasia verruciformis
    W Preiser
    Department of Virology, Royal Free and University College Medical School, University College London, UK
    J Clin Virol 16:55-7. 2000
    ....
  23. pmc Molecular and biological characterization of human monoclonal antibodies binding to the spike and nucleocapsid proteins of severe acute respiratory syndrome coronavirus
    Edward N van den Brink
    Crucell Holland BV, PO Box 2048, 2301 CA Leiden, The Netherlands
    J Virol 79:1635-44. 2005
    ..The complete set of SARS-CoV MAbs described here may be useful for diagnosis, chemoprophylaxis, and therapy of SARS-CoV infection and disease...
  24. ncbi request reprint Comparative evaluation of the Cobas Amplicor HIV-1 Monitor Ultrasensitive Test, the new Cobas AmpliPrep/Cobas Amplicor HIV-1 Monitor Ultrasensitive Test and the Versant HIV RNA 3.0 assays for quantitation of HIV-1 RNA in plasma samples
    Annemarie Berger
    Institute for Medical Virology, J W Goethe University Hospital, Paul Ehrlich Street 40, D 60596 Frankfurt am Main, Germany
    J Clin Virol 33:43-51. 2005
    ..There are several commercially available assays for the quantitation of HIV RNA. A new automated specimen preparation system, the Cobas AmpliPrep, was developed to automate this last part of the PCR...
  25. ncbi request reprint HBV reactivation after kidney transplantation
    Annemarie Berger
    Institute for Medical Virology, Center of Internal Medicine, J W Goethe University Hospital, Paul Ehrlich Str 40, D 60596 Frankfurt a M, Germany
    J Clin Virol 32:162-5. 2005
    ..Periodic follow-up of HBV serology for early diagnosis of reactivation is highly recommended in transplant recipients...
  26. ncbi request reprint Development of antiviral therapy for severe acute respiratory syndrome
    Jindrich Cinatl
    Institut fur Medizinische Virologie, Klinikum der Johann Wolfgang Goethe Universitat, Paul Ehrlich Str 40, 60596 Frankfurt am Main, Germany
    Antiviral Res 66:81-97. 2005
    ..In this review, experimental and clinical data about potential anti-SARS drugs is summarised and discussed. Animal model studies will be needed to help to determine which interventions warrant controlled clinical testing...
  27. ncbi request reprint Cytomegalovirus-specific CD4 T-cell and glycoprotein B specific antibody response in recipients of allogenic stem cell transplantation
    Beatrice Ludwig
    Institute for Medical Virology, Johann Wolfgang Goethe University Frankfurt, Paul Ehrlich Str 40, D 60596 Frankfurt am Main, Germany
    J Clin Virol 35:160-6. 2006
    ..The human cytomegalovirus (HCMV) causes severe complications in immunosuppressed patients, resulting in increased morbidity and mortality. The immunological components important for the control of HCMV are still not completely understood...
  28. ncbi request reprint Ultrasensitive monitoring of HIV-1 viral load by a low-cost real-time reverse transcription-PCR assay with internal control for the 5' long terminal repeat domain
    Christian Drosten
    Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    Clin Chem 52:1258-66. 2006
    ..In some countries, monitoring of antiretroviral therapy is now more expensive than treatment itself. In addition, some commercial assays have shown shortcomings in quantifying rare genotypes...
  29. pmc Human monoclonal antibody combination against SARS coronavirus: synergy and coverage of escape mutants
    Jan Ter Meulen
    Crucell Holland B V, Leiden, Netherlands
    PLoS Med 3:e237. 2006
    ..Combinations of virus-neutralizing, noncompeting mAbs may have these properties...
  30. pmc HIV-1 viral load assays for resource-limited settings: clades matter
    Wolfgang Preiser
    PLoS Med 3:e538; author reply e550. 2006
  31. ncbi request reprint Molecular analysis of HIV type 1 vif sequences from Cape Town, South Africa
    Graeme B Jacobs
    Division of Medical Virology, National Health Laboratory Service NHLS and University of Stellenbosch, Tygerberg 7505, Cape Town, South Africa
    AIDS Res Hum Retroviruses 24:991-4. 2008
    ..The previously identified RLRR (90-93) motif does not seem to be conserved among our newly analyzed sequences. Conserved motifs can be useful for developing new vaccine strategies or antiretroviral drugs...
  32. ncbi request reprint Phylogenetic diversity and low level antiretroviral resistance mutations in HIV type 1 treatment-naive patients from Cape Town, South Africa
    Graeme B Jacobs
    Division of Medical Virology, National Health Laboratory Service NHLS and University of Stellenbosch, Tygerberg 7505, Cape Town, South Africa
    AIDS Res Hum Retroviruses 24:1009-12. 2008
    ..6%) had resistance-associated mutations. These include three (2.1%) NNRTI mutations. With the progression of the national ART program, it is important to monitor the resistance profile of naive and treatment-experienced patients...
  33. ncbi request reprint Phylogenetic analysis of HIV-1 transmission: pol gene sequences are insufficient to clarify true relationships between patient isolates
    Martin Stürmer
    J W Goethe University Hospital, Institute for Medical Virology, Frankfurt, Germany
    AIDS 18:2109-13. 2004
  34. ncbi request reprint Viral genome quantification as a tool for improving patient management: the example of HIV, HBV, HCV and CMV
    Annemarie Berger
    Institute for Medical Virology, Klinikum der J W Goethe Universitat, Paul Ehrlich Strasse 40, D 60596 Frankfurt am Main, Germany
    J Antimicrob Chemother 49:713-21. 2002
    ....
  35. ncbi request reprint Evaluation of the Cobas AmpliPrep/Cobas Amplicor HIV-1 Monitor Ultrasensitive Test: comparison with the Cobas Amplicor HIV-1 Monitor test (manual specimen preparation)
    Annemarie Berger
    Institute for Medical Virology, J W Goethe University Hospital, Paul Ehrlich Street 40, D 60596 Frankfurt Main, Germany
    J Clin Virol 25:S103-7. 2002
    ..In comparison to the assay with manual extraction, no significant difference in HIV-1 RNA copy numbers was observed...
  36. ncbi request reprint Identification of a novel coronavirus in patients with severe acute respiratory syndrome
    Christian Drosten
    Bernhard Nocht Institute for Tropical Medicine, National Reference Center for Tropical Infectious Diseases, Hamburg, Germany
    N Engl J Med 348:1967-76. 2003
    ..The severe acute respiratory syndrome (SARS) has recently been identified as a new clinical entity. SARS is thought to be caused by an unknown infectious agent...
  37. pmc Evaluation of advanced reverse transcription-PCR assays and an alternative PCR target region for detection of severe acute respiratory syndrome-associated coronavirus
    Christian Drosten
    Department of Virology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    J Clin Microbiol 42:2043-7. 2004
    ..Methods for the routine sampling of sputum without infection risk are needed to improve SARS RT-PCR...
  38. ncbi request reprint NAT screening of blood donors for severe acute respiratory syndrome coronavirus can potentially prevent transfusion associated transmissions
    Michael Schmidt
    Institute of Transfusion Medicine and Immunohematology, German Red Cross, Johan Wolfgang Goethe University, Frankfurt, Germany
    Transfusion 44:470-5. 2004
    ..The severe acute respiratory syndrome (SARS) was first described in February 2003. Close contact with symptomatic patients appears to be the main route of transmission, whereas blood transfusion transmission could not be ruled out...
  39. ncbi request reprint HIV-1 genotyping: comparison of two commercially available assays
    Martin Stürmer
    Institute for Medical Virology, University Clinic Frankfurt, Paul Ehrlich Str 40, 60596 Frankfurt, Germany
    Expert Rev Mol Diagn 4:281-91. 2004
    ..A final decision on which system to use must be based on an individual's requirements. The future of this field may lie with the use of microarray systems...
  40. ncbi request reprint Comparison of nine resistance interpretation systems for HIV-1 genotyping
    Martin Stürmer
    Institut fur Medizinische Virologie, Johann Wolfgang Goethe Universität Frankfurt am Main, Germany
    Antivir Ther 8:239-44. 2003
    ..If this interpretation system was omitted, the overall discordance rate decreased by a statistically significant degree. There exists, therefore, an urgent need for standardization of interpretation algorithms for HIV-1 genotyping...
  41. pmc Isolation and characterization of human monoclonal antibodies from individuals infected with West Nile Virus
    Mark Throsby
    Crucell Holland B V, P O Box 2048, 2301 CA, Leiden, The Netherlands
    J Virol 80:6982-92. 2006
    ....
  42. pmc Role of China in the quest to define and control severe acute respiratory syndrome
    Robert F Breiman
    International Centre for Diarrheal Disease Research, Bangladesh Centre for Health and Population Research, Dhaka, Bangladesh
    Emerg Infect Dis 9:1037-41. 2003
    ..China will also be able to contribute key data about how the causative agent is transmitted and how it is evolving, as well as identifying pivotal factors influencing disease outcome...
  43. ncbi request reprint Anti-HIV, anti-poxvirus, and anti-SARS activity of a nontoxic, acidic plant extract from the Trifollium species Secomet-V/anti-vac suggests that it contains a novel broad-spectrum antiviral
    Girish J Kotwal
    Division of Medical Virology, IIDMM, Faculty of Health Sciences, University of Cape Town, Observatory, Cape Town 7925, South Africa
    Ann N Y Acad Sci 1056:293-302. 2005
    ..Secomet-V, therefore, is a broad-spectrum antiviral, which possibly works by neutralizing viral infectivity, resulting in the prevention of viral attachment...
  44. ncbi request reprint Response to 'Single phylogenetic reconstruction method is insufficient to clarify relationships between patient isolates in HIV-1 transmission case' by Jenwitheesuk and Liu
    Martin Stürmer
    AIDS 19:741-3; author reply 743-4. 2005
  45. ncbi request reprint Severe acute respiratory syndrome: identification of the etiological agent
    Christian Drosten
    Department of Virology, Bernhard Nocht Institute for Tropical Medicine, Bernhard Nocht Strasse 74, 20359 Hamburg, Germany
    Trends Mol Med 9:325-7. 2003
  46. ncbi request reprint Human monoclonal antibody as prophylaxis for SARS coronavirus infection in ferrets
    Jan Ter Meulen
    Crucell Holland BV, Leiden, Netherlands
    Lancet 363:2139-41. 2004
    ..The data generated in this animal model show that administration of a human monoclonal antibody might offer a feasible and effective prophylaxis for the control of human SARS coronavirus infection...
  47. ncbi request reprint Bioterrorism: is it a real threat?
    Rene Gottschalk
    Center of Competence for Highly Infectious Diseases, Hesse, Germany
    Med Microbiol Immunol 194:109-14. 2005
    ..Great efforts on the part of governments and public health authorities are necessary to counteract these threats...