Genomes and Genes
Affiliation: Frankfurt am Main
- Varicelloviruses avoid T cell recognition by UL49.5-mediated inactivation of the transporter associated with antigen processingDanijela Koppers-Lalic
Department of Medical Microbiology, Leiden University Medical Center, 2300 RC, Leiden, The Netherlands
Proc Natl Acad Sci U S A 102:5144-9. 2005..UL49.5 is degraded along with TAP via a reaction that requires the cytoplasmic tail of UL49.5. Thus, UL49.5 represents a unique immune evasion protein that inactivates TAP through a unique two-tiered process...
- The varicellovirus-encoded TAP inhibitor UL49.5 regulates the presentation of CTL epitopes by Qa-1b1Thorbald van Hall
Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Albinusdreef 2, Leiden, The Netherlands
J Immunol 178:657-62. 2007..Our data show that the varicellovirus UL49.5 protein is a universal TAP inhibitor that can be exploited for preclinical studies on CTL-based immune intervention...
- The C terminus of the Alb3 membrane insertase recruits cpSRP43 to the thylakoid membraneSebastian Falk
Heidelberg University Biochemistry Center BZH, INF 328, D 69120 Heidelberg, Germany
J Biol Chem 285:5954-62. 2010..Alb3 is adapted for the chloroplast-specific Alb3-cpSRP43 interaction in post-translational targeting by extending the spectrum of chromodomain interactions...
- Activating natural cytotoxicity receptors of natural killer cells in cancer and infectionJoachim Koch
Georg Speyer Haus, Institute for Biomedical Research, Paul Ehrlich Strasse 42 44, D 60596 Frankfurt am Main, Germany
Trends Immunol 34:182-91. 2013..Therefore, a molecular understanding of the function of the NCRs in immunosurveillance is instrumental to discovering novel access points to combat infections and cancer...
- The first N-terminal transmembrane helix of each subunit of the antigenic peptide transporter TAP is essential for independent tapasin bindingJoachim Koch
Institute of Biochemistry, Biocenter, Johann Wolfgang Goethe University Frankfurt, Marie Curie Strasse 9, D 69439 Frankfurt a M, Germany
FEBS Lett 580:4091-6. 2006..Moreover, tapasin binding is dependent on the first N-terminal transmembrane helix of TAP1 and TAP2, demonstrating that these two helices contribute independently to the recruitment of tapasin and associated factors...
- Human recombinant neutralizing antibodies against hantaan virus G2 proteinJoachim Koch
Hantavirus Forschungsstelle der Heidelberger Akademie der Wissenschaften, Im Neuenheimer Feld 230, D 69120 Heidelberg, Germany
Virology 308:64-73. 2003..The neutralizing antibodies to the most widely distributed hantaviruses causing HFRS might be promising candidates for the development of an agent for prevention and treatment of HFRS in patients...
- Membrane topology of the transporter associated with antigen processing (TAP1) within an assembled functional peptide-loading complexSusanne Schrodt
Institute of Biochemistry, Biocenter, Goethe University Frankfurt, Marie Curie Strasse 9, D 60439 Frankfurt Main, Germany
J Biol Chem 281:6455-62. 2006..This study provides a first map of the structural organization of the TAP machinery within the macromolecular MHCI peptide-loading complex...
- Functional dissection of the transmembrane domains of the transporter associated with antigen processing (TAP)Joachim Koch
Institute of Biochemistry, Biocenter, Johann Wolfgang Goethe University Frankfurt, Germany
J Biol Chem 279:10142-7. 2004..Moreover, we demonstrate that the N-terminal domains of TAP1 and TAP2 are essential for recruitment of tapasin, consequently mediating assembly of the macromolecular peptide-loading complex...
- Modulation of the antigenic peptide transporter TAP by recombinant antibodies binding to the last five residues of TAP1Gabriele Plewnia
Institute of Biochemistry, Biocenter, Johann Wolfgang Goethe University, Max von Laue Strasse 9, D 69438 Frankfurt a M, Germany
J Mol Biol 369:95-107. 2007..Based on our results we suggest that the C terminus of TAP1 modulates TAP function presumably as part of the dimer interface of the NBDs...
- Exploring the minimal functional unit of the transporter associated with antigen processingJoachim Koch
Institute of Biochemistry, Biocenter, Johann Wolfgang Goethe University Frankfurt, Marie Curie Strasse 9, D 60439 Frankfurt a M, Germany
FEBS Lett 579:4413-6. 2005..The TM1 of both, core-TAP1 and core-TAP2 are critical for heterodimerization of the complex...
- Epstein-Barr viral BNLF2a protein hijacks the tail-anchored protein insertion machinery to block antigen processing by the transport complex TAPAgnes I Wycisk
Institute of Biochemistry, Biocenter, Goethe University Frankfurt, Max von Laue Str 9, 60438 Frankfurt, Germany
J Biol Chem 286:41402-12. 2011..The inhibition mechanism of EBV BNLF2a is distinct and mutually exclusive of other viral TAP inhibitors...
- Signaling of a varicelloviral factor across the endoplasmic reticulum membrane induces destruction of the peptide-loading complex and immune evasionSandra Loch
Institute of Biochemistry, Biocenter, Goethe University Frankfurt, D 60438, Frankfurt Main, Germany
J Biol Chem 283:13428-36. 2008..5. Within this new immune evasion mechanism, we show for the first time that additive elements of a small viral factor and their signaling across the ER membrane are essential for targeted degradation of a multi-subunit membrane complex...
- Single residue within the antigen translocation complex TAP controls the epitope repertoire by stabilizing a receptive conformationChristoph Baldauf
Institute of Biochemistry, Center for Membrane Proteomics, and Cluster of Excellence Frankfurt Macromolecular Complexes, Goethe University Frankfurt, Max von Laue Strasse 9, D 60438 Frankfurt Main, Germany
Proc Natl Acad Sci U S A 107:9135-40. 2010..As part of an unexpected mechanism, this residue is crucial in complementing the binding pocket for a given subset of epitopes as well as in maintaining a substrate-receptive conformation of the translocation complex...
- Homo-oligomerization of the activating natural killer cell receptor NKp30 ectodomain increases its binding affinity for cellular ligandsJulia Herrmann
From the NK Cell Biology, Georg Speyer Haus, Institute for Tumor Biology and Experimental Therapy, D 60596 Frankfurt am Main, Germany and
J Biol Chem 289:765-77. 2014....
- A bispecific transmembrane antibody simultaneously targeting intra- and extracellular epitopes of the epidermal growth factor receptor inhibits receptor activation and tumor cell growthNina Muller
Georg Speyer Haus, Institute for Tumor Biology and Experimental Therapy, Frankfurt am Main, Germany
Int J Cancer 134:2547-59. 2014..Our data demonstrate functionality of this novel type of membrane-anchored intrabodies in tumor cells and suggest distinct modes of action of mono- and bispecific variants...
- Identification of a new epitope for HIV-neutralizing antibodies in the gp41 membrane proximal external region by an Env-tailored phage display libraryMingkui Zhou
Georg Speyer Haus, Institute for Biomedical Research, Frankfurt, Germany
Eur J Immunol 43:499-509. 2013..Thus, this new epitope represents a promising candidate for further analysis in view of HIV vaccine development...
- Regulatory properties and interaction of the C- and N-terminal domains of BetP, an osmoregulated betaine transporter from Corynebacterium glutamicumVera Ott
Institute of Biochemistry, University of Cologne, 50674 Cologne, Germany, Institute of Biochemistry, University of Frankfurt, 60438 Frankfurt, Germany
Biochemistry 47:12208-18. 2008..These results support a functional model of BetP activation that involves the C-terminal domain shifting from interaction with the membrane to interaction with intramolecular domains in response to osmotic stress...
- Peptide ligands selected with CD4-induced epitopes on native dualtropic HIV-1 envelope proteins mimic extracellular coreceptor domains and bind to HIV-1 gp120 independently of coreceptor usageXavier Dervillez
Georg Speyer Haus, Institute for Biomedical Research, Paul Ehrlich Str 42 44, 60596 Frankfurt am Main, Germany
J Virol 84:10131-8. 2010....
- Engineering ATPase activity in the isolated ABC cassette of human TAP1Robert Ernst
Institute of Biochemistry, Heinrich Heine University Duesseldorf, Universitaetsstrasse 1, 40225 Duesseldorf, Germany
J Biol Chem 281:27471-80. 2006..Based on our results a catalytic hierarchy of these two fundamental amino acids in ATP hydrolysis of the mutated TAP1 motor domain was deduced...
- Dimer-tetramer transition controls RUNX1/ETO leukemogenic activityChristian Wichmann
Institute for Biomedical Research, Georg Speyer Haus, Paul Ehrlich Strasse 42 44, Frankfurt, Germany
Blood 116:603-13. 2010..Our data reveal the existence of an essential structural motif (hot spot) at the NHR2 dimer-tetramer interface, suitable for a molecular intervention in t(8;21) leukemias...
- Crystal structure of the human Fe65-PTB1 domainJens Radzimanowski
Heidelberg University Biochemistry Center, INF328, D 69120 Heidelberg, Germany
J Biol Chem 283:23113-20. 2008..We suggest Fe65-PTB1 to interact with its target proteins involved in translocation and signaling of APP in a phosphorylation-dependent manner...
- Cytotoxicity and infiltration of human NK cells in in vivo-like tumor spheroidsAriane Giannattasio
NK Cell Biology, Georg Speyer Haus, Institute for Tumor Biology and Experimental Therapy, Frankfurt, Germany
BMC Cancer 15:351. 2015..However, cellular in vitro assays, which rely on monolayer cultures of mammalian cell lines, neglect the three-dimensional architecture of a tumor, thus limiting their validity for the in vivo situation...
- Functional characterization of two scFv-Fc antibodies from an HIV controller selected on soluble HIV-1 Env complexes: a neutralizing V3- and a trimer-specific gp41 antibodyMaria Trott
Georg Speyer Haus, Institute for Tumor Biology and Experimental Therapy, Frankfurt, Germany
PLoS ONE 9:e97478. 2014..In conclusion, HIV controllers are a valuable source for the selection of functionally interesting antibodies that can be selected on soluble gp140 proteins with properties from the native envelope spike. ..
- Peptides as drugs: from screening to applicationUrsula Dietrich
Georg Speyer Haus, Institute of Biomedical Research, Paul Ehrlich Strasse 42 44, D 60596 Frankfurt am Main, Germany
Curr Pharm Biotechnol 14:501-12. 2013..This review will summarize the broad potential of peptides as drugs, with special emphasis on peptides which inhibit protein-protein interactions. ..
- The stalk domain and the glycosylation status of the activating natural killer cell receptor NKp30 are important for ligand bindingJessica Hartmann
Georg Speyer Haus, Institute of Biomedical Research, D 60596 Frankfurt am Main, Germany
J Biol Chem 287:31527-39. 2012....
- Mechanisms of tumor and viral immune escape from natural killer cell-mediated surveillanceAriane Groth
Institute of Biomedical Research, Georg Speyer Haus, Frankfurt am Main, Germany
J Innate Immun 3:344-54. 2011..Finally, the review discusses the future perspectives and hypotheses aiming to improve therapeutic NK cell strategies against tumor immune escape mechanisms...
- Natural killer cells in HIV-1 infection: a double-edged swordJessica Funke
Georg Speyer Haus, Institute of Biomedical Research, Frankfurt am Main, Germany
AIDS Rev 13:67-76. 2011..A detailed knowledge of these immune escape strategies might lead to the identification of access points for intervention in order to block infection and progression to AIDS...
- Inhibition of HIV-1 by a peptide ligand of the genomic RNA packaging signal PsiJulia Dietz
Georg Speyer Haus, Institute for Biomedical Research, Paul Ehrlich Str 42 44, 60596 Frankfurt, Germany
ChemMedChem 3:749-55. 2008..Thus, this peptide shows antiviral activity and could serve as a lead compound to develop new drugs targeting HIV-1...
- Varicellovirus UL 49.5 proteins differentially affect the function of the transporter associated with antigen processing, TAPDanijela Koppers-Lalic
Center of Infectious Diseases and Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands
PLoS Pathog 4:e1000080. 2008..5. Taken together, these results classify the UL 49.5 gene products of BHV-1, PRV, EHV-1, and EHV-4 as members of a novel family of viral immune evasion proteins, inhibiting TAP through a variety of mechanisms...
- Functional cysteine-less subunits of the transporter associated with antigen processing (TAP1 and TAP2) by de novo gene assemblySusanne Heintke
Institut fur Biochemie, Biozentrum, Johann Wolfgang Goethe Universitat Frankfurt, Marie Curie Str 9, D 60439, Frankfurt M, Germany
FEBS Lett 533:42-6. 2003..Cysteine-less TAP1 and TAP2 restore maturation and intracellular trafficking of MHC class I molecules to the cell surface...
- Generation of an HFRS patient-derived neutralizing recombinant antibody to Hantaan virus G1 protein and definition of the neutralizing domainMifang Liang
Institute of Virology, Chinese Academy of Preventive Medicine, Beijing, China
J Med Virol 69:99-107. 2003..These results are the first to define a neutralizing epitope on the G1 protein of HTNV using an antibody derived from an HFRS patient...
- Identification of two interaction sites in SecY that are important for the functional interaction with SecAEli O van der Sluis
Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9751 NN Haren, The Netherlands
J Mol Biol 361:839-49. 2006..Our data explain how conformational changes in SecA could be directly coupled to the previously proposed opening mechanism of the SecYEG channel...
- cTAGE: a cutaneous T cell lymphoma associated antigen family with tumor-specific splicingDirk Usener
Skin Cancer Unit, German Cancer Research Center, Heidelberg
J Invest Dermatol 121:198-206. 2003..We conclude that cTAGE-1 and cTAGE-5 are new cancer germline antigens and that tumor-specific splicing of cTAGE genes may lead to further candidate proteins for specific immunotherapy of cutaneous T cell lymphoma and other malignancies...
- Structure and dynamics of membrane-associated ICP47, a viral inhibitor of the MHC I antigen-processing machineryChristopher Aisenbrey
Institut Faculté de Chimie, Université Louis Pasteur CNRS LC3 Unité Mixte de Recherche 7177, 4 rue Blaise Pascal, Strasbourg 67070, France
J Biol Chem 281:30365-72. 2006..These novel insights into the structure of ICP47 represent an important step toward a molecular understanding of the immune evasion mechanism of herpes simplex virus and are instrumental for the design of new therapeutics...