Affiliation: European Molecular Biology Laboratory
- Reverse transfection on cell arrays for high content screening microscopyHolger Erfle
MitoCheck Project Group, EMBL, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
Nat Protoc 2:392-9. 2007..Different human cell lines that have been successfully transfected using the protocol are presented here. The present protocol has been applied to two genome-wide siRNA screens addressing mitosis and constitutive protein secretion...
- High-throughput RNAi screening by time-lapse imaging of live human cellsBeate Neumann
MitoCheck Project Group, European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
Nat Methods 3:385-90. 2006..This modular platform is scalable and makes the power of time-lapse microscopy available for genome-wide RNAi screens...
- Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genesBeate Neumann
MitoCheck Project Group, European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
Nature 464:721-7. 2010..As part of the Mitocheck consortium, this study provides an in-depth analysis of cell division phenotypes and makes the entire high-content data set available as a resource to the community...
- Work flow for multiplexing siRNA assays by solid-phase reverse transfection in multiwell platesHolger Erfle
Cell Biology Biophysics Unit, EMBL, Heidelberg, Germany
J Biomol Screen 13:575-80. 2008..We conclude that solid-phase reverse transfection in multiwell plates is a cost-efficient and flexible tool for multiplexing cellular assays...
- An RNAi screening platform to identify secretion machinery in mammalian cellsJeremy C Simpson
Cell Biology and Biophysics Unit, European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany
J Biotechnol 129:352-65. 2007..Correlating these findings with other screens and bioinformatic information makes these candidates highly likely to be novel membrane traffic machinery components...
- A microscope-based screening platform for large-scale functional protein analysis in intact cellsUrban Liebel
Cell Biology and Cell Biophysics Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
FEBS Lett 554:394-8. 2003..Two automated functional assays addressing protein secretion and the integrity of the Golgi complex were developed and tested. This shows the potential of the system in large-scale, cell-based functional proteomic projects...
- Robust RNAi enhancement via human Argonaute-2 overexpression from plasmids, viral vectors and cell linesKathleen Börner
Department of Infectious Diseases, Virology, Heidelberg University Hospital, Im Neuenheimer Feld 324, D 69120 Heidelberg, Germany, Cluster of Excellence CellNetworks, Heidelberg University, Im Neuenheimer Feld 267, D 69120 Heidelberg, Germany, Department of Medicine III, University Hospital Aachen, Pauwelstrasse 30, D 52074 Aachen, Germany, Department of Infectious Diseases, Molecular Virology, Heidelberg University, Im Neuenheimer Feld 345, D 69120 Heidelberg, Germany, Division Signaling and Functional Genomics, German Cancer Research Center, Im Neuenheimer Feld 580, D 69120 Heidelberg, Germany, BIOQUANT, Heidelberg University, Im Neuenheimer Feld 267, D 69120 Heidelberg, Germany and Department of Pediatric Oncology, Hematology, Immunology and Pulmonology, Heidelberg University Hospital, Im Neuenheimer Feld 350, D 69120 Heidelberg, Germany
Nucleic Acids Res 41:e199. 2013..Our customizable reagents and avenues should broadly improve future in vitro and in vivo RNAi experiments in mammalian systems. ..
- Arrays of transfected mammalian cells for high content screening microscopyHolger Erfle
Cell Biology and Cell Biophysics Program, European Molecular Biology Laboratory, Heidelberg, Germany
Methods Enzymol 404:1-8. 2005..The method is suitable for high content screening microscopy at a high spatial and temporal resolution allowing even time-lapse analysis of hundreds of samples in parallel...
- siRNA cell arrays for high-content screening microscopyHolger Erfle
European Molecular Biology Laboratory, Heidelberg, Germany
Biotechniques 37:454-8, 460, 462. 2004..We demonstrate such an siRNA cell array in a microscope-based functional assay in living cells to determine the effect of various siRNA oligonucleotides against endogenous targets on cellular secretion...
- Transfected cell microarrays: an efficient tool for high-throughput functional analysisVytaute Starkuviene
European Molecular Biology Laboratory, Cell Biology and Cell Biophysics Unit, Heidelberg, Germany
Expert Rev Proteomics 4:479-89. 2007..These advances, combined with an increasing number and diversity of gene perturbing systems, such as RNAi and ectopic gene expression, provide tools for expanding our understanding of biology at the systems level...
- Production of siRNA- and cDNA-transfected cell arrays on noncoated chambered coverglass for high-content screening microscopy in living cellsHolger Erfle
Cell Biology and Cell Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany
Methods Mol Biol 360:155-61. 2007..This method should facilitate drug target validation by small-interfering RNAs...
- High throughput screening of gene functions in mammalian cells using reversely transfected cell arrays: review and protocolMichael Sturzl
Division of Molecular and Experimental Surgery, Department of Surgery, University of Erlangen Nuremberg, Schwabachanlage 10, D 91054 Erlangen, Germany
Comb Chem High Throughput Screen 11:159-72. 2008..Several different cell types have been successfully used for this procedure. This review summarizes the presently available knowledge on this technique and provides a laboratory protocol...
- High-content screening microscopy identifies novel proteins with a putative role in secretory membrane trafficVytaute Starkuviene
Cell Biology and Biophysics Programme, European Molecular Biology Laboratory EMBL, 69117 Heidelberg, Germany
Genome Res 14:1948-56. 2004..It will also serve as an example for similar microscope-based screens addressing different biological questions...
- Automatic identification of subcellular phenotypes on human cell arraysChristian Conrad
Intelligent Bioinformatics Systems, German Cancer Research Center DKFZ, 69120 Heidelberg, Germany
Genome Res 14:1130-6. 2004..Our classification method can be adapted to virtually any microscopic assay based on cell morphology, opening a wide range of applications including large-scale RNAi screening in human cells...