Hannu Myllykallio

Summary

Country: France

Publications

  1. ncbi request reprint An alternative flavin-dependent mechanism for thymidylate synthesis
    Hannu Myllykallio
    Institut de Genetique et Microbiologie, CNRS UMR 8621, Universite Paris Sud, Orsay, France
    Science 297:105-7. 2002
  2. ncbi request reprint Life without dihydrofolate reductase FolA
    Hannu Myllykallio
    Institut de Génétique et Microbiologie CNRS UMR8621, Universite de Paris Sud, Orsay, France
    Trends Microbiol 11:220-3. 2003
  3. pmc Functional evidence for active site location of tetrameric thymidylate synthase X at the interphase of three monomers
    Damien Leduc
    Universite Paris Sud, Institut de Genetique et Microbiologie, Centre National de la Recherche Scientifique Unité Mixte de Recherche 8621, F 91405 Orsay cedex, France
    Proc Natl Acad Sci U S A 101:7252-7. 2004
  4. pmc Genetic and physical mapping of DNA replication origins in Haloferax volcanii
    Cedric Norais
    Institut de Genetique et Microbiologie, Universite Paris Sud, Orsay, France
    PLoS Genet 3:e77. 2007
  5. pmc Targeting of Helicobacter pylori thymidylate synthase ThyX by non-mitotoxic hydroxy-naphthoquinones
    Stephane Skouloubris
    Laboratoire d Optique et Biosciences, CNRS UMR7645, INSERM U1182, Ecole Polytechnique, Palaiseau 91128, France Department of Biology, Universite Paris Sud, Orsay 91405, France
    Open Biol 5:150015. 2015
  6. pmc The structure of an archaeal homodimeric ligase which has RNA circularization activity
    Mark Adrian Brooks
    IBBMC CNRS, Universite de Paris Sud, CNRS UMR8619, IFR115, 91405 Orsay, France
    Protein Sci 17:1336-45. 2008
  7. pmc Identification of short 'eukaryotic' Okazaki fragments synthesized from a prokaryotic replication origin
    Fujihiko Matsunaga
    Universite Paris Sud, Orsay, France
    EMBO Rep 4:154-8. 2003
  8. pmc Identification of a novel gene encoding a flavin-dependent tRNA:m5U methyltransferase in bacteria--evolutionary implications
    Jaunius Urbonavicius
    CNRS, Enzymology and Structural Biochemistry Laboratory 1 avenue de la Terrasse, F 91198, Gif sur Yvette, France
    Nucleic Acids Res 33:3955-64. 2005
  9. pmc Flavin-dependent thymidylate synthase X limits chromosomal DNA replication
    Frédéric Escartin
    Laboratoire d Optique et Biosciences, Centre National de la Recherche Scientifique, Ecole Polytechnique, F 91128 Palaiseau, France
    Proc Natl Acad Sci U S A 105:9948-52. 2008
  10. ncbi request reprint Identification, purification, and characterization of an eukaryotic-like phosphopantetheine adenylyltransferase (coenzyme A biosynthetic pathway) in the hyperthermophilic archaeon Pyrococcus abyssi
    Jean Armengaud
    CEA VALRHO, DSV DIEP, SBTN, Service de Biochimie post génomique and Toxicologie Nucléaire, 30207 Bagnols sur Ceze, France
    J Biol Chem 278:31078-87. 2003

Collaborators

  • Patrick Forterre
  • Jonathan Filee
  • Hilde De Reuse
  • Herman van Tilbeurgh
  • Jonathan A Eisen
  • T Allers
  • A G Barbour
  • H Frederik Nijhout
  • Marc Graille
  • Jean Armengaud
  • Stephane Skouloubris
  • Ursula Liebl
  • Damien Leduc
  • Sébastien Graziani
  • Jaunius Urbonavicius
  • Frédéric Escartin
  • Cedric Norais
  • Fujihiko Matsunaga
  • F Esra Onen
  • Mark Adrian Brooks
  • Yap Boum
  • Jonathan E Ulmer
  • Henri Grosjean
  • Jianmin Zhong
  • Christophe Marchand
  • Edmond Jolivet
  • Karine Anger
  • Kamel Djaout
  • Julien Briffotaux
  • Jean Christophe Lambry
  • Isabelle Lamarre
  • Joelle Kühn
  • Carol Hopkins Sibley
  • Nada Jaber
  • Karine Blondeau
  • Claire Jacquement
  • Daniel Scherman
  • Laurence Meslet-Cladiere
  • Jean Herscovici
  • Christopher D Thouvenel
  • Maria Vittoria Spanedda
  • Amber L Hartman
  • Michael C Reed
  • Celine Brochier-Armanet
  • Michelle Hawkins
  • Julie Bernauer
  • Qiyuan Dai
  • Cong Zhao Zhou
  • Paulette Decottignies
  • James R Gurnon
  • Pierre Le Marechal
  • Gerard Lipowski
  • James L Van Etten
  • Yuannan Xia
  • Daniel Prieur
  • Yoshizumi Ishino

Detail Information

Publications18

  1. ncbi request reprint An alternative flavin-dependent mechanism for thymidylate synthesis
    Hannu Myllykallio
    Institut de Genetique et Microbiologie, CNRS UMR 8621, Universite Paris Sud, Orsay, France
    Science 297:105-7. 2002
    ..Our findings reveal complexity in the evolution of thymidine in present-day DNA. Because ThyX proteins are found in many pathogenic microbes, they present a previously uncharacterized target for antimicrobial compounds...
  2. ncbi request reprint Life without dihydrofolate reductase FolA
    Hannu Myllykallio
    Institut de Génétique et Microbiologie CNRS UMR8621, Universite de Paris Sud, Orsay, France
    Trends Microbiol 11:220-3. 2003
    ....
  3. pmc Functional evidence for active site location of tetrameric thymidylate synthase X at the interphase of three monomers
    Damien Leduc
    Universite Paris Sud, Institut de Genetique et Microbiologie, Centre National de la Recherche Scientifique Unité Mixte de Recherche 8621, F 91405 Orsay cedex, France
    Proc Natl Acad Sci U S A 101:7252-7. 2004
    ....
  4. pmc Genetic and physical mapping of DNA replication origins in Haloferax volcanii
    Cedric Norais
    Institut de Genetique et Microbiologie, Universite Paris Sud, Orsay, France
    PLoS Genet 3:e77. 2007
    ..Our observations also suggest that H. volcanii is an ideal organism for studying how replication of four replicons is regulated in the context of the archaeal cell cycle...
  5. pmc Targeting of Helicobacter pylori thymidylate synthase ThyX by non-mitotoxic hydroxy-naphthoquinones
    Stephane Skouloubris
    Laboratoire d Optique et Biosciences, CNRS UMR7645, INSERM U1182, Ecole Polytechnique, Palaiseau 91128, France Department of Biology, Universite Paris Sud, Orsay 91405, France
    Open Biol 5:150015. 2015
    ..We also demonstrate that chemical reactivity of NQs does not prevent their exploitation as anti-microbial compounds, particularly when mitotoxicity screening is used to prioritize these compounds for further experimentation. ..
  6. pmc The structure of an archaeal homodimeric ligase which has RNA circularization activity
    Mark Adrian Brooks
    IBBMC CNRS, Universite de Paris Sud, CNRS UMR8619, IFR115, 91405 Orsay, France
    Protein Sci 17:1336-45. 2008
    ..Additionally, we show that the protein is indeed capable of circularizing RNA molecules in an ATP-dependent reaction. These structural and biochemical results provide an insight into the potential physiological roles of Pab1020...
  7. pmc Identification of short 'eukaryotic' Okazaki fragments synthesized from a prokaryotic replication origin
    Fujihiko Matsunaga
    Universite Paris Sud, Orsay, France
    EMBO Rep 4:154-8. 2003
    ..Short Okazaki fragments and a high replication speed imply a very efficient turnover of Okazaki fragments in Archaea. Archaea therefore have a unique replication system showing mechanistic similarities to both Bacteria and Eukarya...
  8. pmc Identification of a novel gene encoding a flavin-dependent tRNA:m5U methyltransferase in bacteria--evolutionary implications
    Jaunius Urbonavicius
    CNRS, Enzymology and Structural Biochemistry Laboratory 1 avenue de la Terrasse, F 91198, Gif sur Yvette, France
    Nucleic Acids Res 33:3955-64. 2005
    ....
  9. pmc Flavin-dependent thymidylate synthase X limits chromosomal DNA replication
    Frédéric Escartin
    Laboratoire d Optique et Biosciences, Centre National de la Recherche Scientifique, Ecole Polytechnique, F 91128 Palaiseau, France
    Proc Natl Acad Sci U S A 105:9948-52. 2008
    ..We also propose that the increased kinetic efficiency of thymidylate synthesis has contributed to extending the prokaryotic evolutionary potential...
  10. ncbi request reprint Identification, purification, and characterization of an eukaryotic-like phosphopantetheine adenylyltransferase (coenzyme A biosynthetic pathway) in the hyperthermophilic archaeon Pyrococcus abyssi
    Jean Armengaud
    CEA VALRHO, DSV DIEP, SBTN, Service de Biochimie post génomique and Toxicologie Nucléaire, 30207 Bagnols sur Ceze, France
    J Biol Chem 278:31078-87. 2003
    ..The existence of operational proteins highly conserved between Archaea and Eukarya involved in a central metabolic pathway challenge evolutionary scenarios in which eukaryal operational proteins are strictly of bacterial origin...
  11. ncbi request reprint Functional analysis of FAD-dependent thymidylate synthase ThyX from Paramecium bursaria Chlorella virus-1
    Sébastien Graziani
    Laboratory of Optics and Biosciences, INSERM U451 CNRS UMR 7645, Ecole Polytechnique, 91128 Palaiseau Cedex, France
    J Biol Chem 279:54340-7. 2004
    ..This oxidation activity is directly linked to FAD reduction. Our results indicate that ThyX-specific inhibitors can be designed that do not affect ThyA enzymes. Finally, a model is proposed for the early stages of ThyX catalysis...
  12. pmc Flavin-dependent thymidylate synthase ThyX activity: implications for the folate cycle in bacteria
    Damien Leduc
    Inserm Avenir Group, Institut de Génétique et de Microbiologie, CNRS UMR8621, F 91405 Orsay, France
    J Bacteriol 189:8537-45. 2007
    ..In addition, our observations suggest that the presence of flavin-dependent thymidylate synthase X provides growth benefits under conditions in which the level of reduced folate derivatives is compromised...
  13. pmc Functional analysis of the Mycobacterium tuberculosis FAD-dependent thymidylate synthase, ThyX, reveals new amino acid residues contributing to an extended ThyX motif
    Jonathan E Ulmer
    Department of Genome Sciences, University of Washington, Seattle, WA 98195 5065, USA
    J Bacteriol 190:2056-64. 2008
    ..ThyX is found in a variety of bacterial pathogens but is absent in humans, which depend upon an unrelated thymidylate synthase, ThyA. Therefore, ThyX is a potential target for development of antibacterial drugs...
  14. ncbi request reprint Catalytic mechanism and structure of viral flavin-dependent thymidylate synthase ThyX
    Sébastien Graziani
    CNRS, UMR 7645, Laboratory of Optics and Biosciences, Ecole Polytechnique, 91128 Palaiseau, France
    J Biol Chem 281:24048-57. 2006
    ..Steady-state kinetic analyses support a reaction mechanism where ThyX catalysis proceeds via formation of distinct ternary complexes without formation of a methyl enzyme intermediate...
  15. doi request reprint Design, synthesis and evaluation of potent thymidylate synthase X inhibitors
    F Esra Onen
    Unite de Pharmacologie Chimique et Genetique, Ecole Nationale Superieure de Chimie de Paris, Paris, France
    Bioorg Med Chem Lett 18:3628-31. 2008
    ....
  16. ncbi request reprint In vitro detection of the enzymatic activity of folate-dependent tRNA (Uracil-54,-C5)-methyltransferase: evolutionary implications
    Jaunius Urbonavicius
    Laboratoire d Enzymologie et Biochimie Structurales, Gif sur Yvette, France
    Methods Enzymol 425:103-19. 2007
    ..The phylogenetic analysis of TrmFO sequences suggests an ancient origin of this enzyme in bacteria...
  17. pmc Function and evolution of plasmid-borne genes for pyrimidine biosynthesis in Borrelia spp
    Jianmin Zhong
    Departments of Microbiology and of Molecular Genetics and Medicine, University of California Irvine, Irvine, CA 92697 4028, USA
    J Bacteriol 188:909-18. 2006
    ..These findings indicate that Borrelia spp. causing RF have a greater capability for de novo pyrimidine synthesis than those causing LB, thus providing a basis for some of the biological differences between the two groups of pathogens...
  18. pmc Physiological responses of the hyperthermophilic archaeon "Pyrococcus abyssi" to DNA damage caused by ionizing radiation
    Edmond Jolivet
    Institut Universitaire Europeen de la Mer, Universite de Bretagne Occidentale, CNRS UMR 6539, Technopole Brest Iroise, 29 280 Plouzane, France
    J Bacteriol 185:3958-61. 2003
    ..Our cell fractionation assays further suggest that "P. abyssi" contains a highly efficient DNA repair system which is continuously ready to repair the DNA damage caused by high temperature and/or ionizing radiation...