M C Marden

Summary

Country: France

Publications

  1. pmc Control of the allosteric equilibrium of hemoglobin by cross-linking agents
    Michael C Marden
    INSERM U473, Hopital de Bicetre, 94276 Le Kremlin Bicetre Cedex, France
    Protein Sci 11:1376-83. 2002
  2. pmc Neuroglobin and other hexacoordinated hemoglobins show a weak temperature dependence of oxygen binding
    Julien Uzan
    INSERM U473, 94276 Le Kremlin Bicetre, France
    Biophys J 87:1196-204. 2004
  3. ncbi request reprint Exploiting a list of protein sequences
    Michael C Marden
    Department of Biomedical Sciences, University of Antwerp, B 2610 Antwerp, Belgium
    Gene 398:35-41. 2007
  4. ncbi request reprint Modulation of the oxygen affinity of cobalt-porphyrin by globin
    M C Marden
    INSERM U473, 84 rue du General Leclerc, 94276, Le Kremlin Bicetre, France
    FEBS Lett 472:221-4. 2000
  5. ncbi request reprint Identifying the conformational state of bi-liganded haemoglobin
    M C Marden
    INSERM U473, Le Kremlin Bicetre, France
    Cell Mol Life Sci 54:1365-84. 1998
  6. ncbi request reprint Additive effects of beta chain mutations in low oxygen affinity hemoglobin betaF41Y,K66T
    V Baudin-Creuza
    INSERM, Unité 473, 84 rue du General Leclerc, 94276 Le Kremlin Bicetre Cedex, France
    J Biol Chem 274:25550-4. 1999
  7. ncbi request reprint Biochemical characterization and ligand binding properties of neuroglobin, a novel member of the globin family
    S DeWilde
    Department of Biochemistry, University of Antwerp, B 2610 Antwerp, Belgium
    J Biol Chem 276:38949-55. 2001
  8. ncbi request reprint Fitting abnormal oxygen equilibrium curves of hemoglobin
    M C Marden
    INSERM U299, Hopital de Bicetre, France
    Biophys Chem 37:363-9. 1990
  9. ncbi request reprint Functional properties of hemoglobin in human red cells: II. Determination of the Bohr effect
    J Kister
    INSERM U 299, Hopital de Bicetre, Le Kremlin Bicetre, France
    Respir Physiol 73:363-78. 1988
  10. ncbi request reprint The redox state of the cell regulates the ligand binding affinity of human neuroglobin and cytoglobin
    Djemel Hamdane
    INSERM U473, 94276 Le Kremlin Bicetre Cedex, France
    J Biol Chem 278:51713-21. 2003

Collaborators

Detail Information

Publications47

  1. pmc Control of the allosteric equilibrium of hemoglobin by cross-linking agents
    Michael C Marden
    INSERM U473, Hopital de Bicetre, 94276 Le Kremlin Bicetre Cedex, France
    Protein Sci 11:1376-83. 2002
    ....
  2. pmc Neuroglobin and other hexacoordinated hemoglobins show a weak temperature dependence of oxygen binding
    Julien Uzan
    INSERM U473, 94276 Le Kremlin Bicetre, France
    Biophys J 87:1196-204. 2004
    ..This effect could be of biological relevance for certain organisms, since it could provide a globin with an oxygen affinity that is nearly independent of temperature...
  3. ncbi request reprint Exploiting a list of protein sequences
    Michael C Marden
    Department of Biomedical Sciences, University of Antwerp, B 2610 Antwerp, Belgium
    Gene 398:35-41. 2007
    ..Examples are shown based on globin sequences from various species and on the abnormal human hemoglobins. The software should be of interest to protein engineers who need to know what variants are already known...
  4. ncbi request reprint Modulation of the oxygen affinity of cobalt-porphyrin by globin
    M C Marden
    INSERM U473, 84 rue du General Leclerc, 94276, Le Kremlin Bicetre, France
    FEBS Lett 472:221-4. 2000
    ..The time constants for autoxidation at 37 degrees C were 250 and 100 h for Pe and Gc, respectively...
  5. ncbi request reprint Identifying the conformational state of bi-liganded haemoglobin
    M C Marden
    INSERM U473, Le Kremlin Bicetre, France
    Cell Mol Life Sci 54:1365-84. 1998
    ..1992). Science 255: 54-63]. The implications of this model and the relevant experiments will be reviewed here...
  6. ncbi request reprint Additive effects of beta chain mutations in low oxygen affinity hemoglobin betaF41Y,K66T
    V Baudin-Creuza
    INSERM, Unité 473, 84 rue du General Leclerc, 94276 Le Kremlin Bicetre Cedex, France
    J Biol Chem 274:25550-4. 1999
    ..Despite the lower oxygen affinity, the single mutant betaF41Y and double mutant betaF41Y,K66T show only a moderate increase of 20% in the autoxidation rate. These mutations are thus of interest in developing a Hb-based blood substitute...
  7. ncbi request reprint Biochemical characterization and ligand binding properties of neuroglobin, a novel member of the globin family
    S DeWilde
    Department of Biochemistry, University of Antwerp, B 2610 Antwerp, Belgium
    J Biol Chem 276:38949-55. 2001
    ..The biochemical and kinetic characteristics are discussed in view of the possible functions of neuroglobin in the vertebrate brain...
  8. ncbi request reprint Fitting abnormal oxygen equilibrium curves of hemoglobin
    M C Marden
    INSERM U299, Hopital de Bicetre, France
    Biophys Chem 37:363-9. 1990
    ..In this way the effect on the equilibrium due to perturbations in energy of each substate can be studied as a diagnostic tool...
  9. ncbi request reprint Functional properties of hemoglobin in human red cells: II. Determination of the Bohr effect
    J Kister
    INSERM U 299, Hopital de Bicetre, Le Kremlin Bicetre, France
    Respir Physiol 73:363-78. 1988
    ..These results indicate that the hydration of the Hb molecules in the highly concentrated red cell milieu is not much different from that of nearly ideal solutions...
  10. ncbi request reprint The redox state of the cell regulates the ligand binding affinity of human neuroglobin and cytoglobin
    Djemel Hamdane
    INSERM U473, 94276 Le Kremlin Bicetre Cedex, France
    J Biol Chem 278:51713-21. 2003
    ..Hence the oxygen affinity would be directly linked to the redox state of the cell...
  11. ncbi request reprint Hyperthermal stability of neuroglobin and cytoglobin
    Djemel Hamdane
    INSERM U473, Le Kremlin Bicetre, France
    FEBS J 272:2076-84. 2005
    ....
  12. ncbi request reprint High pressure enhances hexacoordination in neuroglobin and other globins
    Djemel Hamdane
    INSERM U473, 78 rue du General Leclerc, 94275 Le Kremlin Bicetre, France
    J Biol Chem 280:36809-14. 2005
    ....
  13. doi request reprint Cytoglobin conformations and disulfide bond formation
    Christophe Lechauve
    INSERM U779, Universités Paris VI et XI, Le Kremlin Bicetre, France
    FEBS J 277:2696-704. 2010
    ..An internal disulfide bond may form which modifies the rate of dissociation of the distal histidine and apparently leads to different cytoglobin conformations, which may affect the observed oxygen affinity by an order of magnitude...
  14. ncbi request reprint Transfer of human alpha- to beta-hemoglobin via its chaperone protein: evidence for a new state
    Veronique Baudin-Creuza
    INSERM U473, 78 rue du General Leclerc, 94275 Le Kremlin Bicetre cedex, France
    J Biol Chem 279:36530-3. 2004
    ..The physiologically relevant replacement of the chaperone by beta-hemoglobin chains could be detected by both ligand binding kinetics and tryptophan fluorescence quenching...
  15. doi request reprint Structure and function evolution in the superfamily of globins
    Henri Wajcman
    INSERM U841, Equipe 11, CHU Henri Mondor, 94010 Creteil, France
    C R Biol 332:273-82. 2009
    ..All these aspects are discussed in this review...
  16. pmc Hemoglobin Porto Alegre forms a tetramer of tetramers superstructure
    Veronique Baudin-Creuza
    INSERM, Unité 473, 94276 Le Kremlin Bicetre Cedex, France
    Protein Sci 11:129-36. 2002
    ..None of the other variants investigated formed such a large oligomer...
  17. doi request reprint Artificial oxygen carrier based on polysaccharides-poly(alkylcyanoacrylates) nanoparticle templates
    Cedric Chauvierre
    Institut National de la Sante et de la Recherche Medicale, INSERM U779, Le Kremlin Bicetre, France
    Biomaterials 31:6069-74. 2010
    ..Such nanoparticles could be a suitable alternative to haemoglobin solutions in the development of a blood substitute...
  18. doi request reprint Construction of a new polycistronic vector for over-expression and rapid purification of human hemoglobin
    Elisa Domingues
    INSERM U779, University of Paris VII and XI, Le Kremlin Bicetre, France
    Plasmid 61:71-7. 2009
    ..This polycistronic vector system, which offers the additional advantage of a very rapid purification, is especially well suited for the study of abnormal, unstable globins in order to better understand the associated pathology...
  19. pmc The hemoglobins of the trematodes Fasciola hepatica and Paramphistomum epiclitum: a molecular biological, physico-chemical, kinetic, and vaccination study
    Sylvia Dewilde
    Department of Biomedical Sciences, University of Antwerp, Universiteitsplein 1, B 2610 Antwerp, Belgium
    Protein Sci 17:1653-62. 2008
    ..he. HbF2 failed to promote protection against parasitic infection...
  20. ncbi request reprint Nitric oxide binding properties of neuroglobin. A characterization by EPR and flash photolysis
    Sabine Van Doorslaer
    Department of Physics, University of Antwerp, B 2610 Antwerp, Belgium
    J Biol Chem 278:4919-25. 2003
    ..Structural and kinetic characteristics of neuroglobin and its mutants are analyzed. NO production in rapidly growing E. coli cell cultures is discussed...
  21. pmc Heme as an optical probe of a conformational transition of ovine recPrP
    Christine Pato
    INSERM, U473, 84 rue du General Leclerc, 94276 Le Kremlin Bicetre Cedex, France
    Protein Sci 13:1100-7. 2004
    ..This would indicate that the peptide may alter or inhibit the polymer formation...
  22. pmc Structure and function of the globin and globin gene from the Antarctic mollusc Yoldia eightsi
    Sylvia Dewilde
    Department of Biochemistry, University of Antwerp, Universiteitsplein 1, 2610 Antwerp, Belgium
    Biochem J 370:245-53. 2003
    ..2 intron. Phylogenetic analyses reveal a gene tree where the Yoldia haemoglobin gene is separated from other mollusc globin genes, confirming the specific adaptation of the Yoldia haemoglobin...
  23. ncbi request reprint Hb Val de Marne [alpha 133(H16)Ser-->Arg]: a new hemoglobin variant with moderate increase in oxygen affinity
    H Wajcman
    INSERM U 299, Hopital de Bicetre, France
    Hemoglobin 17:407-17. 1993
    ..Hb Val de Marne, isolated by isoelectrofocusing, displayed a slightly higher auto-oxidation rate than Hb A. Its oxygen affinity was increased 1.7-fold in comparison to that of Hb A. The heterotropic effects were normal...
  24. ncbi request reprint The nerve hemoglobin of the bivalve mollusc Spisula solidissima: molecular cloning, ligand binding studies, and phylogenetic analysis
    Sylvia Dewilde
    Department of Biomedical Sciences, University of Antwerp, Campus Drie Eiken, Universiteitsplein 1, B 2610 Antwerp, Belgium
    J Biol Chem 281:5364-72. 2006
    ..They most likely fulfill a myoglobin-like function and enhance oxygen supply to the neurons...
  25. ncbi request reprint High-yield expression in Escherichia coli of soluble human alpha-hemoglobin complexed with its molecular chaperone
    Corinne Vasseur-Godbillon
    INSERM U473, Le Kremlin Bicetre, France
    Protein Eng Des Sel 19:91-7. 2006
    ....
  26. pmc Characterization of nonsymbiotic tomato hemoglobin
    A Iulia Ioanitescu
    Departments of Physics and Biomedical Sciences, University of Antwerp, Antwerp, Belgium
    Biophys J 89:2628-39. 2005
    ..The similarities and differences with other known nonsymbiotic plant hemoglobins will be highlighted...
  27. doi request reprint Neuroglobin and prion cellular localization: investigation of a potential interaction
    Christophe Lechauve
    INSERM U779, Universités Paris VI et XI, Hopital de Bicetre, Le Kremlin Bicetre, France
    J Mol Biol 388:968-77. 2009
    ....
  28. pmc Globin-like proteins in Caenorhabditis elegans: in vivo localization, ligand binding and structural properties
    Eva Geuens
    Department of Biomedical Sciences, University of Antwerp, Universiteitsplein 1, B 2610 Antwerp, Belgium
    BMC Biochem 11:17. 2010
    ..No information is available on the physicochemical properties and the in vivo expression...
  29. ncbi request reprint Enhancing the tolerance of poly(isobutylcyanoacrylate) nanoparticles with a modular surface design
    Cedric Chauvierre
    INSERM U779, Hopital de Bicetre, Secteur Broca, 78 rue du General Leclerc, 94275 Le Kremlin Bicetre, France
    Int J Pharm 338:327-32. 2007
    ..It was found that the tolerance of PIBCA nanoparticles by cells could be improved up to 100-fold by coating their surface with polysaccharides and haemoglobin...
  30. doi request reprint Expression, purification, and crystallization of neuro- and cytoglobin
    Sylvia Dewilde
    Department of Biomedical Sciences, University of Antwerp, Antwerp, Belgium
    Methods Enzymol 436:341-57. 2008
    ..coli and its preparative purification. These proteins are then used in crystallization experiments. Also an analytical purification strategy is discussed in detail...
  31. ncbi request reprint Beta7E-beta132K salt bridge and sickle haemoglobin stability and conformation
    Christophe Fablet
    Institut National de la Sante et de la Recherche Medicale, Unité 473, Le Kremlin Bicetre, France
    Br J Haematol 122:317-25. 2003
    ....
  32. ncbi request reprint Ligand binding dynamics to the heme domain of the oxygen sensor Dos from Escherichia coli
    Ursula Liebl
    Laboratory for Optical Biosciences, INSERM U451, CNRS UMR 7645, Ecole Polytechnique ENSTA, 91128 Palaiseau Cedex, France
    Biochemistry 42:6527-35. 2003
    ..Further remarkable observations include evidence for stable (>4 ns) photooxidation of six-coordinate ferrous heme, with a quantum yield of 4-8%...
  33. ncbi request reprint Synthesis and X-ray studies of chiral allosteric modifiers of hemoglobin
    Amal Mamdouh Youssef
    Department of Medicinal Chemistry, School of Pharmacy, Institute for Structural Biology and Drug Discovery, Virginia Commonwealth University, Richmond, Virginia 23298 0133, USA
    J Med Chem 45:1184-95. 2002
    ..The most potent effectors, in vitro, were compounds 15 and 19, D-isomers of leucine and phenylalanine, respectively. Compounds 21, 22, 30, and32 were more potent in vitro in Hb solutions than JP7...
  34. ncbi request reprint Coupling of the heme and an internal disulfide bond in human neuroglobin
    Djemel Hamdane
    INSERM U473, 84 rue du General Leclerc, 94276 Le Kremlin Bicetre, Cedex France
    Micron 35:59-62. 2004
    ..This suggests a novel mechanism for the oxygen binding; reduction of the disulfide bond would provoke the release of oxygen...
  35. ncbi request reprint Characterization of Drosophila hemoglobin. Evidence for hemoglobin-mediated respiration in insects
    Thomas Hankeln
    Institute of Molecular Genetics, Biosafety Research and Consulting, University of Mainz, D 55099 Mainz, Germany
    J Biol Chem 277:29012-7. 2002
    ..This suggests that oxygen supply in insects may be more complex than thought previously and may depend on hemoglobin-mediated oxygen transport and storage in addition to simple diffusion...
  36. ncbi request reprint Role of arginine 220 in the oxygen sensor FixL from Bradyrhizobium japonicum
    Veronique Balland
    Laboratoire de Biophysique du Stress Oxydant, SBE DBJC and CNRS URA 2096, CEA Saclay, 91191 Gif sur Yvette Cedex, France
    J Biol Chem 280:15279-88. 2005
    ....
  37. ncbi request reprint Reversible hexacoordination of alpha-hemoglobin-stabilizing protein (AHSP)/alpha-hemoglobin Versus pressure. Evidence for protection of the alpha-chains by their chaperone
    Djemel Hamdane
    INSERM U779, University of Paris XI, 94275 Le Kremlin Bicetre, France
    J Biol Chem 282:6398-404. 2007
    ..The reaction with hydrogen peroxide of ferric alpha-Hb within the complex also demonstrates a protection against aggregation...
  38. ncbi request reprint Impaired binding of AHSP to alpha chain variants: Hb Groene Hart illustrates a mechanism leading to unstable hemoglobins with alpha thalassemic like syndrome
    Corinne Vasseur-Godbillon
    INSERM Unité 779, 78, rue du General Leclerc, 94275 Le Kremlin Bicetre cedex, France
    Blood Cells Mol Dis 37:173-9. 2006
    ..The CO rebinding kinetics of the AHSP/alpha-Hb(42mutant) complexes were similar to those previously obtained with the AHSP/alpha-Hb(WT) complex, which shows a modified rate that is intermediate to the classical Hb allosteric states...
  39. pmc Stable octameric structure of recombinant hemoglobin alpha(2)beta(2)83 Gly-->Cys
    Christophe Fablet
    INSERM U 473, 94276 Le Kremlin Bicetre Cedex, France
    Protein Sci 12:690-5. 2003
    ..However, the size of oligomer betaG83C was not modified after incubation with fresh plasma...
  40. ncbi request reprint The homozygous state for Hb Crete [beta129 (H7) Ala-->Pro] is associated with a complex phenotype including erythrocytosis and functional anemia
    Ioannis Papassotiriou
    Department of Clinical Biochemistry, Aghia Sophia Children s Hospital, 115 27 Athens, Greece
    Blood Cells Mol Dis 34:229-34. 2005
    ..The structural change in Hb Crete only partly explains all the pathological manifestations of this variant, and other mechanisms are discussed...
  41. ncbi request reprint Recombinant hemoglobin betaG83C-F41Y
    Corinne Vasseur-Godbillon
    INSERM Unité 473, Le Kremlin Bicetre, France
    FEBS J 273:230-41. 2006
    ..In addition, the octameric rHb betaG83C-F41Y was resistant to potential reducing agents present in fresh plasma...
  42. ncbi request reprint Hemoglobin I from Lucina pectinata: a model for distal heme-ligand control
    Ruth Pietri
    Department of Chemistry, University of Puerto Rico, Mayaguez Campus, PO Box 9019, Mayagüez 00681 9019, Puerto Rico
    Biochim Biophys Acta 1764:758-65. 2006
    ..Here, a mechanism in which the flexibility of the distal protein matrix coupled with hemeporphyrin movement toward a different configuration is suggested as an important process in the H2S transport and delivery in hemoglobin I...
  43. ncbi request reprint Zebrafish reveals different and conserved features of vertebrate neuroglobin gene structure, expression pattern, and ligand binding
    Christine Fuchs
    Institute of Molecular Genetics, Johannes Gutenberg University of Mainz, D 55099 Mainz, Germany
    J Biol Chem 279:24116-22. 2004
    ..Neuroglobin appears to be associated with mitochondria-rich cell types and thus oxygen consumption rates, suggesting a myoglobin-like function of this protein in facilitated oxygen diffusion...
  44. ncbi request reprint Ex vivo generation of fully mature human red blood cells from hematopoietic stem cells
    Marie Catherine Giarratana
    Laboratoire d Hématologie EA1638, Universite Paris VI, CHU Saint Antoine, 27 rue de Chaligny 75571 Paris Cedex 12, France
    Nat Biotechnol 23:69-74. 2005
    ..Further development of large-scale production of cultured RBCs will have implications for gene therapy, blood transfusion and tropical medicine...
  45. ncbi request reprint Human erythroid cells produced ex vivo at large scale differentiate into red blood cells in vivo
    Thi My Anh Neildez-Nguyen
    Institut National de la Santé et de la Recherche Médicale U417, Hopital Saint Antoine, Paris, France
    Nat Biotechnol 20:467-72. 2002
    ..The approach may eventually be useful in clinical transfusion applications...
  46. doi request reprint Ultrafast heme-residue bond formation in six-coordinate heme proteins: implications for functional ligand exchange
    Marten H Vos
    Laboratoire d Optique et Biosciences, CNRS, Ecole Polytechnique, F 91128 Palaiseau, France
    Biochemistry 47:5718-23. 2008
    ..to functionally replace the internal residue after thermal dissociation of the heme-residue bond...
  47. ncbi request reprint Neuroglobin and cytoglobin in search of their role in the vertebrate globin family
    Thomas Hankeln
    Institute of Molecular Genetics, Johannes Gutenberg University of Mainz, D 55099 Mainz, Germany
    J Inorg Biochem 99:110-9. 2005
    ..Cytoglobin is predominantly expressed in fibroblasts and related cell types, but also in distinct nerve cell populations. Much less is known about its function, although in fibroblasts it might be involved in collagen synthesis...