Nicolas Berthet

Summary

Affiliation: Institut Pasteur
Country: France

Publications

  1. pmc Phi29 polymerase based random amplification of viral RNA as an alternative to random RT-PCR
    Nicolas Berthet
    1Genotyping of Pathogens and Public Health Technological Platform, Institut Pasteur, Paris, France
    BMC Mol Biol 9:77. 2008
  2. pmc Application of high-density DNA resequencing microarray for detection and characterization of botulinum neurotoxin-producing clostridia
    Jessica Vanhomwegen
    Institut Pasteur, Laboratory for Urgent Responses to Biological Threats, Paris, France
    PLoS ONE 8:e67510. 2013
  3. pmc Evaluation of high-throughput sequencing for identifying known and unknown viruses in biological samples
    Justine Cheval
    Institut Pasteur, Genotyping of Pathogens and Public Health Platform, 28 rue du Docteur Roux, F 75015 Paris, France
    J Clin Microbiol 49:3268-75. 2011
  4. pmc Use of consensus sequences for the design of high density resequencing microarrays: the influenza virus paradigm
    India Leclercq
    Institut Pasteur, Laboratory for Urgent Response to Biological Threats CIBU, Paris, France
    BMC Genomics 11:586. 2010
  5. pmc Massively parallel pathogen identification using high-density microarrays
    Nicolas Berthet
    Genotyping of Pathogens and Public Health Technological Platform, Institut Pasteur, Paris, France
    Microb Biotechnol 1:79-86. 2008
  6. pmc Improved assembly procedure of viral RNA genomes amplified with Phi29 polymerase from new generation sequencing data
    Nicolas Berthet
    Institut Pasteur, Epidemiology and Physiopathology of Oncogenic Viruses, 25 rue du Docteur Roux, 75724, Paris, France
    Biol Res 49:39. 2016
  7. doi request reprint Complete Genome Characterization of the Arumowot Virus (Unclassified Phlebovirus) Isolated from Turdus libonyanus Birds in the Central African Republic
    Nicolas Berthet
    1 Institut Pasteur, Epidemiology and Physiopathology of Oncogenic Viruses, Paris, France
    Vector Borne Zoonotic Dis 16:139-43. 2016
  8. pmc Application of broad-spectrum resequencing microarray for genotyping rhabdoviruses
    Laurent Dacheux
    Institut Pasteur, Lyssavirus Dynamics and Host Adaptation Unit, Paris, France
    J Virol 84:9557-74. 2010
  9. pmc No evidence for viral sequences in five lepidic adenocarcinomas (former "BAC") by a high-throughput sequencing approach
    Nicolas Berthet
    Epidemiology and Physiopathology of Oncogenic Viruses Unit, Institut Pasteur, 28 rue du Docteur Roux, 75015, Paris, France
    BMC Res Notes 8:782. 2015
  10. pmc Recurrent Breast Abscesses due to Corynebacterium kroppenstedtii, a Human Pathogen Uncommon in Caucasian Women
    Anne Le Flèche-Matéos
    Institut Pasteur, Cellule d Intervention Biologique d Urgence CIBU, 75724 Paris, France
    Case Rep Infect Dis 2012:120968. 2012

Collaborators

Detail Information

Publications11

  1. pmc Phi29 polymerase based random amplification of viral RNA as an alternative to random RT-PCR
    Nicolas Berthet
    1Genotyping of Pathogens and Public Health Technological Platform, Institut Pasteur, Paris, France
    BMC Mol Biol 9:77. 2008
    ..We adapted the QuantiTect Whole Transcriptome Kit (Qiagen) to our purposes and designated the method as Whole Transcriptome Amplification (WTA)...
  2. pmc Application of high-density DNA resequencing microarray for detection and characterization of botulinum neurotoxin-producing clostridia
    Jessica Vanhomwegen
    Institut Pasteur, Laboratory for Urgent Responses to Biological Threats, Paris, France
    PLoS ONE 8:e67510. 2013
    ..The high diversity in nucleotide sequence and genetic organization of the gene cluster encoding the BoNT components poses a great challenge for the screening and characterization of BoNT-producing strains...
  3. pmc Evaluation of high-throughput sequencing for identifying known and unknown viruses in biological samples
    Justine Cheval
    Institut Pasteur, Genotyping of Pathogens and Public Health Platform, 28 rue du Docteur Roux, F 75015 Paris, France
    J Clin Microbiol 49:3268-75. 2011
    ..When the virus load was sufficiently high, de novo assembly permitted the generation of long contigs corresponding to nearly full-length genomes and thus should facilitate the identification of novel viruses...
  4. pmc Use of consensus sequences for the design of high density resequencing microarrays: the influenza virus paradigm
    India Leclercq
    Institut Pasteur, Laboratory for Urgent Response to Biological Threats CIBU, Paris, France
    BMC Genomics 11:586. 2010
    ..The part dedicated to influenza viruses was based on consensus sequences specific for one gene generated from global alignments of a large number of influenza virus sequences available in databanks...
  5. pmc Massively parallel pathogen identification using high-density microarrays
    Nicolas Berthet
    Genotyping of Pathogens and Public Health Technological Platform, Institut Pasteur, Paris, France
    Microb Biotechnol 1:79-86. 2008
    ..Our results suggest this technology could be applied in a clinical setting to test for numerous pathogens in a rapid, sensitive and unbiased manner...
  6. pmc Improved assembly procedure of viral RNA genomes amplified with Phi29 polymerase from new generation sequencing data
    Nicolas Berthet
    Institut Pasteur, Epidemiology and Physiopathology of Oncogenic Viruses, 25 rue du Docteur Roux, 75724, Paris, France
    Biol Res 49:39. 2016
    ..The pre-process method proposed in this study strongly limits the negative impact of chimeric reads in order to obtain the full-length of viral genomes...
  7. doi request reprint Complete Genome Characterization of the Arumowot Virus (Unclassified Phlebovirus) Isolated from Turdus libonyanus Birds in the Central African Republic
    Nicolas Berthet
    1 Institut Pasteur, Epidemiology and Physiopathology of Oncogenic Viruses, Paris, France
    Vector Borne Zoonotic Dis 16:139-43. 2016
    ....
  8. pmc Application of broad-spectrum resequencing microarray for genotyping rhabdoviruses
    Laurent Dacheux
    Institut Pasteur, Lyssavirus Dynamics and Host Adaptation Unit, Paris, France
    J Virol 84:9557-74. 2010
    ....
  9. pmc No evidence for viral sequences in five lepidic adenocarcinomas (former "BAC") by a high-throughput sequencing approach
    Nicolas Berthet
    Epidemiology and Physiopathology of Oncogenic Viruses Unit, Institut Pasteur, 28 rue du Docteur Roux, 75015, Paris, France
    BMC Res Notes 8:782. 2015
    ..Around 180 million single reads of 100 bases were generated for each BAC sample...
  10. pmc Recurrent Breast Abscesses due to Corynebacterium kroppenstedtii, a Human Pathogen Uncommon in Caucasian Women
    Anne Le Flèche-Matéos
    Institut Pasteur, Cellule d Intervention Biologique d Urgence CIBU, 75724 Paris, France
    Case Rep Infect Dis 2012:120968. 2012
    ..The current lack of such investigations, probably due to the difficulty to detect Ck, may cause the underestimation of such an aetiology...
  11. doi request reprint Genetic characterization of Chikungunya virus in the Central African Republic
    Marion Desdouits
    Virology Department, Institut Pasteur de Bangui, Bangui, Central African Republic Epidemiology and Physiopathology of Oncogenic Viruses Unit, Institut Pasteur, Paris, France Electronic address
    Infect Genet Evol 33:25-31. 2015
    ..albopictus, as previously observed in other Central African countries. Intense surveillance of the human and vector populations is necessary to prevent or anticipate the emergence of a massive CHIKF epidemic in the CAR...