Eric Cascales

Summary

Affiliation: Centre National de la Recherche Scientifique
Country: France

Publications

  1. doi request reprint The SciZ protein anchors the enteroaggregative Escherichia coli Type VI secretion system to the cell wall
    Marie Stéphanie Aschtgen
    Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Microbiologie de la Mediterranee, Universite de la Mediterranee Aix Marseille II, CNRS UPR 9027, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Mol Microbiol 75:886-99. 2010
  2. pmc Colicin biology
    Eric Cascales
    Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Biologie Structurale et Microbiologie, Centre National de la Recherche Scientifique, UPR 9027, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Microbiol Mol Biol Rev 71:158-229. 2007
  3. pmc The type VI secretion toolkit
    Eric Cascales
    Laboratoire d Ingenierie des Systemes Macromoleculaires, IBSM, Centre National de la Recherche Scientifique, Marseille, France
    EMBO Rep 9:735-41. 2008
  4. doi request reprint Architecture and assembly of the Type VI secretion system
    Abdelrahim Zoued
    Laboratoire d Ingenierie des Systemes Macromoleculaires, CNRS, Aix Marseille Universite, UMR 7255, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Biochim Biophys Acta 1843:1664-73. 2014
  5. doi request reprint Mapping the interactions between escherichia coli tol subunits: rotation of the TolR transmembrane helix
    Xiang Y Z Zhang
    Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Biologie Structurale et Microbiologie, Centre National de la Recherche Scientifique, UPR 9027, 13402 Marseille, France
    J Biol Chem 284:4275-82. 2009
  6. pmc Interaction of the colicin K bactericidal toxin with components of its import machinery in the periplasm of Escherichia coli
    Aurélie Barnéoud-Arnoulet
    Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Microbiologie de la Mediterranee, Aix Marseille Universite, CNRS UPR 9027, Marseille, France
    J Bacteriol 192:5934-42. 2010
  7. ncbi request reprint Movements of the TolR C-terminal domain depend on TolQR ionizable key residues and regulate activity of the Tol complex
    Emilie L Goemaere
    Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Biologie Structurale et Microbiologie, CNRS, UPR 9027, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Biol Chem 282:17749-57. 2007
  8. pmc Structural biology of type VI secretion systems
    Eric Cascales
    CNRS, Laboratoire d Ingenierie des Systemes Macromoleculaires, UMR 7255, Institut de Microbiologie de la Mediterranee, Aix Marseille Universite, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Philos Trans R Soc Lond B Biol Sci 367:1102-11. 2012
  9. pmc Mapping the interactions between Escherichia coli TolQ transmembrane segments
    Xiang Y Z Zhang
    Laboratoire d Ingénierie des Systèmes Macromoléculaires UPR9027, CNRS, Aix Marseille Universite, Marseille, France
    J Biol Chem 286:11756-64. 2011
  10. pmc TssK is a trimeric cytoplasmic protein interacting with components of both phage-like and membrane anchoring complexes of the type VI secretion system
    Abdelrahim Zoued
    From the Laboratoire d Ingéniérie des Systèmes Macromoléculaires, Institut de Microbiologie de la Mediterranee, CNRS, UMR 7255, Aix Marseille Universite, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France and
    J Biol Chem 288:27031-41. 2013

Collaborators

Detail Information

Publications40

  1. doi request reprint The SciZ protein anchors the enteroaggregative Escherichia coli Type VI secretion system to the cell wall
    Marie Stéphanie Aschtgen
    Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Microbiologie de la Mediterranee, Universite de la Mediterranee Aix Marseille II, CNRS UPR 9027, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Mol Microbiol 75:886-99. 2010
    ..Using in vivo and in vitro binding assays, we showed that this motif anchors the SciZ protein to the cell wall and is required for T6SS function...
  2. pmc Colicin biology
    Eric Cascales
    Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Biologie Structurale et Microbiologie, Centre National de la Recherche Scientifique, UPR 9027, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Microbiol Mol Biol Rev 71:158-229. 2007
    ..The mechanisms of inhibition by specific and cognate immunity proteins are presented. Finally, the use of colicins as laboratory or biotechnological tools and their mode of evolution are discussed...
  3. pmc The type VI secretion toolkit
    Eric Cascales
    Laboratoire d Ingenierie des Systemes Macromoleculaires, IBSM, Centre National de la Recherche Scientifique, Marseille, France
    EMBO Rep 9:735-41. 2008
    ..This review provides an overview of our current knowledge about type VI secretion, focusing on gene regulation, components of the secretion machine, substrate secretion and the cellular consequences for the host cell...
  4. doi request reprint Architecture and assembly of the Type VI secretion system
    Abdelrahim Zoued
    Laboratoire d Ingenierie des Systemes Macromoleculaires, CNRS, Aix Marseille Universite, UMR 7255, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Biochim Biophys Acta 1843:1664-73. 2014
    ..This article is part of a Special Issue entitled: Protein trafficking and secretion in bacteria. Guest Editors: Anastassios Economou and Ross Dalbey. ..
  5. doi request reprint Mapping the interactions between escherichia coli tol subunits: rotation of the TolR transmembrane helix
    Xiang Y Z Zhang
    Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Biologie Structurale et Microbiologie, Centre National de la Recherche Scientifique, UPR 9027, 13402 Marseille, France
    J Biol Chem 284:4275-82. 2009
    ..TolR anchors may form molecular gears that oscillate in response to proton motive force to regulate channel activity...
  6. pmc Interaction of the colicin K bactericidal toxin with components of its import machinery in the periplasm of Escherichia coli
    Aurélie Barnéoud-Arnoulet
    Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Microbiologie de la Mediterranee, Aix Marseille Universite, CNRS UPR 9027, Marseille, France
    J Bacteriol 192:5934-42. 2010
    ..For the first time, we also identified an interaction between the TolQ protein and a colicin translocation domain...
  7. ncbi request reprint Movements of the TolR C-terminal domain depend on TolQR ionizable key residues and regulate activity of the Tol complex
    Emilie L Goemaere
    Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Biologie Structurale et Microbiologie, CNRS, UPR 9027, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Biol Chem 282:17749-57. 2007
    ..We monitored these structural changes by cysteine accessibility experiments and showed that the conformation of this domain is responsive to the proton-motive force and on the presence of critical residues of the ion pathway...
  8. pmc Structural biology of type VI secretion systems
    Eric Cascales
    CNRS, Laboratoire d Ingenierie des Systemes Macromoleculaires, UMR 7255, Institut de Microbiologie de la Mediterranee, Aix Marseille Universite, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Philos Trans R Soc Lond B Biol Sci 367:1102-11. 2012
    ..In this review, we summarize recent structural information on individual T6SS components emphasizing the fact that T6SSs are composite systems, adapting subunits from various origins...
  9. pmc Mapping the interactions between Escherichia coli TolQ transmembrane segments
    Xiang Y Z Zhang
    Laboratoire d Ingénierie des Systèmes Macromoléculaires UPR9027, CNRS, Aix Marseille Universite, Marseille, France
    J Biol Chem 286:11756-64. 2011
    ....
  10. pmc TssK is a trimeric cytoplasmic protein interacting with components of both phage-like and membrane anchoring complexes of the type VI secretion system
    Abdelrahim Zoued
    From the Laboratoire d Ingéniérie des Systèmes Macromoléculaires, Institut de Microbiologie de la Mediterranee, CNRS, UMR 7255, Aix Marseille Universite, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France and
    J Biol Chem 288:27031-41. 2013
    ..Fluorescence microscopy experiments further demonstrate the requirement of TssK for sheath assembly. Our results suggest a central role for TssK by linking both complexes during T6SS assembly. ..
  11. pmc An epigenetic switch involving overlapping fur and DNA methylation optimizes expression of a type VI secretion gene cluster
    Yannick R Brunet
    Laboratoire d Ingenierie des Systemes Macromoleculaires, CNRS UPR 9027, Institut de Microbiologie de la Mediterranee, Aix Marseille Universite, Marseille, France
    PLoS Genet 7:e1002205. 2011
    ..A model is proposed in which the sci1 promoter is regulated by iron availability, adenine methylation, and DNA replication...
  12. doi request reprint A phospholipase A1 antibacterial Type VI secretion effector interacts directly with the C-terminal domain of the VgrG spike protein for delivery
    Nicolas Flaugnatti
    Laboratoire d Ingenierie des Systemes Macromoleculaires, CNRS Aix Marseille Universite, UMR 7255, Institut de Microbiologie de la Mediterranee, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Mol Microbiol 99:1099-118. 2016
    ..Based on these results, we propose an additional mechanism of transport of T6SS effectors in which cognate effectors are selected by specific motifs located at the C-terminus of VgrG proteins. ..
  13. pmc Towards a structural comprehension of bacterial type VI secretion systems: characterization of the TssJ-TssM complex of an Escherichia coli pathovar
    Catarina Felisberto-Rodrigues
    Aix Marseille Universite, Architecture et Fonction des Macromolecules Biologiques, Campus de Luminy, Marseille, France
    PLoS Pathog 7:e1002386. 2011
    ..Collectively, our results provide an improved understanding of T6SS assembly and encourage structure-aided drug design of novel antimicrobials targeting T6SS...
  14. pmc Regulation of type VI secretion gene clusters by sigma54 and cognate enhancer binding proteins
    Christophe S Bernard
    Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Microbiologie de la Mediterranee, Aix Marseille Universite, CNRS UPR9027, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Bacteriol 193:2158-67. 2011
    ..Using in vitro binding experiments and in vivo reporter fusion assays, we showed that the expression of these clusters is dependent on both σ(54) and bEBPs...
  15. ncbi request reprint Anchoring the type VI secretion system to the peptidoglycan: TssL, TagL, TagP... what else?
    Marie Stéphanie Aschtgen
    Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Microbiologie de la Mediterranee, Centre National de la Recherche Scientifique CNRS, Aix Marseille Universite, Marseille, France
    Virulence 1:535-40. 2010
    ..Herein, we discuss these results, examine whether PG-binding motifs are found within other T6SS subunits and express hypotheses regarding the role of PG-binding motifs in type VI secretion...
  16. ncbi request reprint Deletion analyses of the peptidoglycan-associated lipoprotein Pal reveals three independent binding sequences including a TolA box
    Eric Cascales
    Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Biologie Structurale et de Microbiologie, CNRS, UPR 9027, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Mol Microbiol 51:873-85. 2004
    ..Furthermore, a conserved polypeptide sequence located downstream of the peptidoglycan binding motif of Pal was required for the TolA-Pal interaction and for the maintenance of outer membrane stability...
  17. pmc Tol-dependent macromolecule import through the Escherichia coli cell envelope requires the presence of an exposed TolA binding motif
    Stéphanie Pommier
    Institut de Biologie Structurale et de Microbiologie, CNRS, UPR 9027, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Bacteriol 187:7526-34. 2005
    ..Furthermore, in vivo and in vitro coprecipitation analyses demonstrated that colicin A and g3p N-terminal domains compete for binding to TolA...
  18. pmc SciN is an outer membrane lipoprotein required for type VI secretion in enteroaggregative Escherichia coli
    Marie Stéphanie Aschtgen
    Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Biologie Structurale et Microbiologie, CNRS, UPR 9027, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Bacteriol 190:7523-31. 2008
    ..Together, these results support a model in which SciN is an outer membrane lipoprotein exposed in the periplasm and essential for the Sci-1 apparatus function...
  19. doi request reprint Biogenesis and structure of a type VI secretion membrane core complex
    Eric Durand
    1 Laboratoire d Ingénierie des Systèmes Macromoléculaires, Aix Marseille Université CNRS, UMR 7255, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France 2 Architecture et Fonction des Macromolécules Biologiques, CNRS, UMR 7257, Campus de Luminy, Case 932, 13288 Marseille Cedex 09, France 3 G5 Biologie structurale de la sécrétion bactérienne, Institut Pasteur, 25 28 Rue du Docteur Roux, 75015 Paris, France 4 UMR 3528, CNRS, Institut Pasteur, 25 28 Rue du Docteur Roux, 75015 Paris, France 5 AFMB, Aix Marseille Universite, IHU Méditerranée Infection, Campus de Luminy, Case 932, 13288 Marseille Cedex 09, France
    Nature 523:555-60. 2015
    ....
  20. pmc Dissection of the TssB-TssC interface during type VI secretion sheath complex formation
    Xiang Y Zhang
    Laboratoire d Ingénierie des Systèmes Macromoléculaires LISM, UMR 7255, Institut de Microbiologie de la Méditerranée IMM, Centre National de la Recherche Scientifique CNRS, Aix Marseille Universite, Marseille, France
    PLoS ONE 8:e81074. 2013
    ..Using site-directed mutagenesis coupled to phenotypic analyses, we demonstrate that an hydrophobic motif located in the N-terminal region of this helix is required for interaction with TssC, sheath assembly and T6SS function. ..
  21. ncbi request reprint Mutational analyses define helix organization and key residues of a bacterial membrane energy-transducing complex
    Emilie L Goemaere
    Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Biologie Structurale et Microbiologie, CNRS UPR 9027, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Mol Biol 366:1424-36. 2007
    ..We discuss a model for the function of related bacterial molecular motors...
  22. doi request reprint Structure-Function Analysis of the TssL Cytoplasmic Domain Reveals a New Interaction between the Type VI Secretion Baseplate and Membrane Complexes
    Abdelrahim Zoued
    Laboratoire d Ingénierie des Systèmes Macromoléculaires LISM, UMR 7255, Institut de Microbiologie de la Méditerranée IMM, Aix Marseille Univ Centre National de la Recherche Scientifique CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Mol Biol 428:4413-4423. 2016
    ..Finally, competition assays demonstrated that these interactions are physiologically important for T6SS function...
  23. doi request reprint Priming and polymerization of a bacterial contractile tail structure
    Abdelrahim Zoued
    Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Microbiologie de la Mediterranee, CNRS UMR7255, Aix Marseille Universite, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Nature 531:59-63. 2016
    ..TssA remains at the tip of the growing structure and incorporates new tube and sheath blocks. On the basis of these results, we propose that TssA primes and coordinates tail tube and sheath biogenesis. ..
  24. pmc Inhibition of type VI secretion by an anti-TssM llama nanobody
    Van Son Nguyen
    Architecture et Fonction des Macromolecules Biologiques, Centre National de la Recherche Scientifique CNRS UMR 7257, Marseille, France Architecture et Fonction des Macromolécules Biologiques, Aix Marseille Universite, Campus de Luminy, Case 932, Marseille, France
    PLoS ONE 10:e0122187. 2015
    ..Indeed, periplasmic production of the nanobodies displacing the TssM-TssJ interaction inhibits the T6SS function in vivo. This study illustrates the power of nanobodies to specifically target and inhibit bacterial secretion systems. ..
  25. pmc Crystal structure and self-interaction of the type VI secretion tail-tube protein from enteroaggregative Escherichia coli
    Badreddine Douzi
    Aix Marseille Universite, Architecture et Fonction des Macromolecules Biologiques, UMR 7257, Campus de Luminy, Case 932, Marseille, France Centre National de la Recherche Scientifique, Architecture et Fonction des Macromolecules Biologiques, UMR 7257, Campus de Luminy, Case 932, Marseille, France
    PLoS ONE 9:e86918. 2014
    ..These results, together with those available in the literature regarding TssB and TssC, suggest that assembly of the T6SS tube differs significantly from that of Sipho- or Myoviridae. ..
  26. ncbi request reprint Analysis of the Escherichia coli Tol-Pal and TonB systems by periplasmic production of Tol, TonB, colicin, or phage capsid soluble domains
    Emmanuelle Bouveret
    LISM, CNRS, 31, Chemin Joseph Aiguier, 13402, Marseille, Cedex 20, France
    Biochimie 84:413-21. 2002
    ..This technique can be used to detect new interactions, to characterize them biochemically and to map them or to induce tol or tonB phenotypes to study the functions of these two complexes...
  27. pmc HxcQ liposecretin is self-piloted to the outer membrane by its N-terminal lipid anchor
    Véronique Viarre
    Laboratoire d Ingénierie des Systèmes Macromoléculaires UPR 9027, CNRS, Institut de Microbiologie de la Mediterranee, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Biol Chem 284:33815-23. 2009
    ..Altogether, our results reveal an original and unprecedented pathway for secretin transport to the OM...
  28. doi request reprint Molecular Dissection of the Interface between the Type VI Secretion TssM Cytoplasmic Domain and the TssG Baseplate Component
    Laureen Logger
    Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Microbiologie de la Mediterranee, Aix Marseille Universite, CNRS UMR 7255, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Mol Biol 428:4424-4437. 2016
    ..Finally, site-directed mutagenesis coupled to functional assays reveals the contribution of these subdomains and conserved motifs to the interaction with T6SS partners and to the function of the secretion apparatus...
  29. pmc The Type VI Secretion TssEFGK-VgrG Phage-Like Baseplate Is Recruited to the TssJLM Membrane Complex via Multiple Contacts and Serves As Assembly Platform for Tail Tube/Sheath Polymerization
    Yannick R Brunet
    Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Microbiologie de la Mediterranee, Aix Marseille Universite, CNRS UMR 7255, Marseille, France
    PLoS Genet 11:e1005545. 2015
    ..Finally, we show that recruitment of the baseplate to the apparatus requires initial positioning of the membrane complex and contacts between TssG and the inner membrane TssM protein. ..
  30. pmc Pal lipoprotein of Escherichia coli plays a major role in outer membrane integrity
    Eric Cascales
    Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Biologie Structurale et de Microbiologie, CNRS, UPR 9027, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Bacteriol 184:754-9. 2002
    ..However, Pal was present in larger amounts compared to TolA and TolR proteins. The oligomeric state of Pal was determined and a new interaction between Pal and Lpp was demonstrated...
  31. doi request reprint VgrG, Tae, Tle, and beyond: the versatile arsenal of Type VI secretion effectors
    Eric Durand
    Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Microbiologie de la Mediterranee, CNRS UMR7255, Aix Marseille Universite, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France Architecture et Fonction des Macromolécules Biologiques, CNRS UMR7257, Aix Marseille Universite, Campus de Luminy, Case 932, 13288 Marseille Cedex 09, France
    Trends Microbiol 22:498-507. 2014
    ....
  32. pmc The C-tail anchored TssL subunit, an essential protein of the enteroaggregative Escherichia coli Sci-1 Type VI secretion system, is inserted by YidC
    Marie Stéphanie Aschtgen
    Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Microbiologie de la Mediterranee, Aix Marseille Université CNRS UMR 7255, 31 Chemin Joseph Aiguier, 13402, Marseille Cedex 20, France
    Microbiologyopen 1:71-82. 2012
    ..We further show that this transmembrane segment is essential for the function of the protein and its proper insertion in the inner membrane is dependent upon YidC and modulated by the Hsp70 homologue DnaK...
  33. pmc Nooks and crannies in type VI secretion regulation
    Christophe S Bernard
    Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Microbiologie de la Mediterranee, Aix Marseille Universite, CNRS UPR 9027, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Bacteriol 192:3850-60. 2010
    ....
  34. pmc Crystallization and preliminary X-ray analysis of the C-terminal fragment of PorM, a subunit of the Porphyromonas gingivalis type IX secretion system
    Julien Stathopulos
    Architecture et Fonction des Macromolécules Biologiques AFMB, UMR 7257 CNRS et Aix Marseille Université, Marseille, France
    Acta Crystallogr F Struct Biol Commun 71:71-4. 2015
    ..Crystals of this fragment belonged to the tetragonal space group P4(3)2(1)2. Native and MAD data sets were recorded to 2.85 and 3.1 Å resolution, respectively, using synchrotron radiation...
  35. pmc H-NS Silencing of the Salmonella Pathogenicity Island 6-Encoded Type VI Secretion System Limits Salmonella enterica Serovar Typhimurium Interbacterial Killing
    Yannick R Brunet
    Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Microbiologie de la Mediterranee, Aix Marseille Universite, CNRS UMR7255, Marseille, France
    Infect Immun 83:2738-50. 2015
    ..Our results suggest that relieving T6SS H-NS silencing may be used as a sense-and-kill mechanism that will help S. Typhimurium to homogenize and synchronize the microbial population to gain efficiency during infection. ..
  36. pmc Type VI secretion and bacteriophage tail tubes share a common assembly pathway
    Yannick R Brunet
    Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Microbiologie de la Méditerranée CNRS UMR 7255 Aix Marseille University, Marseille, France
    EMBO Rep 15:315-21. 2014
    ..Finally, we show that VgrG but not TssB/C controls the proper assembly of the Hcp tubular structure. These results highlight the conservation in the assembly mechanisms between the T6SS and the bacteriophage tail tube/sheath. ..
  37. pmc Promoter swapping unveils the role of the Citrobacter rodentium CTS1 type VI secretion system in interbacterial competition
    Erwan Gueguen
    Laboratoire d Ingénierie des Systèmes Macromoléculaires LISM, Institut de Microbiologie de la Mediterranee, CNRS UMR7255, Aix Marseille University, Marseille, France
    Appl Environ Microbiol 79:32-8. 2013
    ..Artificial induction of both promoters demonstrated that the CTS1 T6SS is functional as shown by the Hcp release assay and confers on C. rodentium a growth advantage in antibacterial competition experiments with Escherichia coli...
  38. pmc The PorX Response Regulator of the Porphyromonas gingivalis PorXY Two-Component System Does Not Directly Regulate the Type IX Secretion Genes but Binds the PorL Subunit
    Maxence S Vincent
    Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Microbiologie de la Mediterranee, Aix Marseille Université Centre National de la Recherche Scientifique UMR7255 Marseille, France
    Front Cell Infect Microbiol 6:96. 2016
    ..Finally, we show that PorX interacts with the cytoplasmic domain of PorL, a component of the T9SS membrane core complex and propose that the CheY-like PorX protein might be involved in the dynamics of the T9SS. ..
  39. pmc Transcriptional frameshifting rescues Citrobacter rodentium type VI secretion by the production of two length variants from the prematurely interrupted tssM gene
    Erwan Gueguen
    Laboratoire d Ingénierie des Systèmes Macromoléculaires LISM, Institut de Microbiologie de la Mediterranee, CNRS Aix Marseille Universite, UMR 7255, Marseille, France
    PLoS Genet 10:e1004869. 2014
    ..rodentium. Finally, we demonstrate that the tssM gene associated with the Yersinia pseudotuberculosis T6SS-3 gene cluster is also subjected to transcriptional frameshifting. ..
  40. doi request reprint Imaging type VI secretion-mediated bacterial killing
    Yannick R Brunet
    Laboratoire d Ingénierie des Systèmes Macromoléculaires LISM, UMR 7255
    Cell Rep 3:36-41. 2013
    ..The results support a model in which T6SS dynamics are responsible for T6SS effectors translocation into recipient cells...